MD09G1210000.v1.1

Late embryogenesis abundant protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr09
OG0002766
Physical Location & Seq
Reverse (-)
20226709 .. 20228762
2054 bp
Loading structure...
UTR
Exon/CDS
Intron
MD09G1210000.v1.1.491

Sequence Viewer

Length: 942 bp
ATGATGCACTCAACAAAATCTGAGTCTGATATCACAAGCTTAGCTGCATCATCACCATCAAGGTCCCCAAAGGCACCTGTGTATTATGTGCAGAGCCCTTCAAGGGACTCACACGATGGGGACAAGTCCTCGTCAATGCATGCCACTCCAATGTACAACAACAGCCCCATGGAGTCACCCTCACACCCCTCTTTCGGCCGCCACTCGAGGAACTCCTCCGCGAGCCGATTTTCTGGGATTTTCAGGTCATCTTCCGGTAGAAAAGGCGGTAGGAAGCCGAGGAATGACAAGGGGTGGCCGGAGTGTAAGGTGATTATGGAGGAAGGGGGTTATGATGATTTCAACGATAAGTCGCTTTCGCGCCGTGTGCAGGTCTTGATTGCTCTTCTTAGCTTTGTGGTGTTGTTCACTGTCTTCTGCTTGATCATATGGGGTGCAAGCAGGCCTTACAAACCAGAAGTTGTAGTTAAGAGCTTGGATATCAGTAACTTCTACATTGGTGAGGGTTCAGACTTCAGTGGAGTTCCAACAAAGCTGGTGACGGTGAATGGCTCATTAAGGCTCAGTGTACGCAACTCTGCTTCAATTTTCGGCATTCACGTTACCTCCTCACCTATCGGTCTCCTCTATTCAGAGATTGCGGTAGCAATTGGTCAGTTGAAGAAATATTATCAGCCAAGAAAGAGCCTCCGAACCATATCAGTAAACTTGCAAGGAACCAAGGTTCCTCTATACGGGGCTGGATCAAGTTTGAAGTACTCAAAGACTGGTGCATTGGTTCCAATTCCGTTGACCTTAAAATTTGAAATCCGGTCTCGAGCGTACGTGGTAGGGAAGCTAGTGAAAACGAAGCGTCGAAGGCGAATCTCTTGTCCTTTGGTTTTGGATCCCACTGTCACCAAACCCATCAAGTTCAATAAGACTTCATGCGCTTATGACTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00260 ko00630 ko01100 ko01110 ko01130 ko01200 map00260 map00630 map01100 map01110 map01130 map01200
Pfam Domains
Protein Families
LEA_2

Protein Analysis

314

Amino Acids

34.44

Weight (kDa)

10.07

Isoelectric Point (pI)

61.74

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 361
AccB1I GGYRCC 1 cut(s) 73
AccII CGCG 2 cut(s) 221, 361
AciI CCGC 4 cut(s) 199, 219, 267, 641
AclWI GGATC 3 cut(s) 751, 881, 894
AcoI YGGCCR 2 cut(s) 196, 296
AcsI RAATTY 1 cut(s) 800
AcuI CTGAAG 1 cut(s) 499
AfaI GTAC 4 cut(s) 155, 570, 758, 824
AfiI CCNNNNNNNGG 4 cut(s) 103, 194, 370, 734
AgsI TTSAA 7 cut(s) 102, 343, 585, 661, 754, 806, 916
AloI GAACNNNNNNTCC 4 cut(s) 708, 709, 740, 741
AluBI AGCT 6 cut(s) 39, 44, 393, 474, 535, 838
AluI AGCT 6 cut(s) 39, 44, 393, 474, 535, 838
Alw26I GTCTC 2 cut(s) 626, 819
AlwI GGATC 3 cut(s) 751, 881, 894
Ama87I CYCGRG 2 cut(s) 205, 816
AoxI GGCC 3 cut(s) 196, 296, 443
ApeKI GCWGC 1 cut(s) 44
ApoI RAATTY 1 cut(s) 800
ArsI GACNNNNNNTTYG 4 cut(s) 8, 40, 856, 888
AspLEI GCGC 2 cut(s) 363, 932
AspS9I GGNCC 1 cut(s) 63
AsuHPI GGTGA 8 cut(s) 45, 168, 322, 512, 550, 556, 603, 889
AvaI CYCGRG 2 cut(s) 205, 816
AvaII GGWCC 1 cut(s) 63
BamHI GGATCC 1 cut(s) 886
BanI GGYRCC 1 cut(s) 73
BanII GRGCYC 1 cut(s) 98
BbsI GAAGAC 1 cut(s) 406
BbvI GCAGC 1 cut(s) 31
BccI CCATC 3 cut(s) 64, 110, 914
BceAI ACGGC 1 cut(s) 348
BclI TGATCA 1 cut(s) 423
BcoDI GTCTC 2 cut(s) 626, 819
BfaI CTAG 1 cut(s) 839
BfuAI ACCTGC 1 cut(s) 361
BisI GCNGC 2 cut(s) 45, 199
BlpI GCTNAGC 1 cut(s) 40
BlsI GCNGC 2 cut(s) 46, 200
BmcAI AGTACT 1 cut(s) 758
Bme18I GGWCC 1 cut(s) 63
BmeT110I CYCGRG 2 cut(s) 205, 816
BmgT120I GGNCC 1 cut(s) 63
BmiI GGNNCC 6 cut(s) 65, 75, 718, 726, 780, 888
BmsI GCATC 1 cut(s) 56
BpiI GAAGAC 1 cut(s) 406
BplI GAGNNNNNCTC 2 cut(s) 164, 196
Bpu1102I GCTNAGC 1 cut(s) 40
BsaAI YACGTR 1 cut(s) 826
BsaI GGTCTC 2 cut(s) 626, 819
BsaJI CCNNGG 3 cut(s) 168, 278, 720
BsaWI WCCGGW 2 cut(s) 254, 810
BsaXI ACNNNNNCTCC 2 cut(s) 590, 620
Bsc4I CCNNNNNNNGG 4 cut(s) 103, 194, 370, 734
Bse1I ACTGG 1 cut(s) 772
BseDI CCNNGG 3 cut(s) 168, 278, 720
BseLI CCNNNNNNNGG 4 cut(s) 103, 194, 370, 734
BseMII CTCAG 2 cut(s) 12, 577
BseNI ACTGG 1 cut(s) 772
BseRI GAGGAG 3 cut(s) 205, 598, 614
BseX3I CGGCCG 1 cut(s) 196
BseXI GCAGC 1 cut(s) 31
BsgI GTGCAG 2 cut(s) 110, 389
Bsh1236I CGCG 2 cut(s) 221, 361
Bsh1285I CGRYCG 1 cut(s) 199
BshFI GGCC 3 cut(s) 198, 298, 445
BshNI GGYRCC 1 cut(s) 73
BsiEI CGRYCG 1 cut(s) 199
BsiHKCI CYCGRG 2 cut(s) 205, 816
BsiSI CCGG 3 cut(s) 255, 299, 811
BsiWI CGTACG 1 cut(s) 822
BslFI GGGAC 3 cut(s) 49, 119, 134
BslI CCNNNNNNNGG 4 cut(s) 103, 194, 370, 734
BsmAI GTCTC 2 cut(s) 626, 819
BsmFI GGGAC 3 cut(s) 49, 119, 134
BsmI GAATGC 1 cut(s) 594
BsnI GGCC 3 cut(s) 198, 298, 445
Bso31I GGTCTC 2 cut(s) 626, 819
BsoBI CYCGRG 2 cut(s) 205, 816
Bsp1286I GDGCHC 1 cut(s) 98
Bsp1407I TGTACA 1 cut(s) 153
Bsp143I GATC 3 cut(s) 423, 743, 886
Bsp1720I GCTNAGC 1 cut(s) 40
Bsp19I CCATGG 1 cut(s) 168
BspACI CCGC 4 cut(s) 199, 219, 267, 641
BspANI GGCC 3 cut(s) 198, 298, 445
BspCNI CTCAG 2 cut(s) 13, 576
BspFNI CGCG 2 cut(s) 221, 361
BspLI GGNNCC 6 cut(s) 65, 75, 718, 726, 780, 888
BspMI ACCTGC 1 cut(s) 361
BspPI GGATC 3 cut(s) 751, 881, 894
BspQI GCTCTTC 1 cut(s) 390
BspT107I GGYRCC 1 cut(s) 73
BspTNI GGTCTC 2 cut(s) 626, 819
BsrGI TGTACA 1 cut(s) 153
BsrI ACTGG 1 cut(s) 772
BssECI CCNNGG 3 cut(s) 168, 278, 720
BssMI GATC 3 cut(s) 423, 743, 886
BssT1I CCWWGG 2 cut(s) 168, 720
Bst4CI ACNGT 3 cut(s) 412, 544, 895
Bst6I CTCTTC 1 cut(s) 390
BstAUI TGTACA 1 cut(s) 153
BstBAI YACGTR 1 cut(s) 826
BstC8I GCNNGC 4 cut(s) 141, 223, 439, 443
BstDEI CTNAG 4 cut(s) 21, 40, 389, 563
BstDSI CCRYGG 1 cut(s) 168
BstFNI CGCG 2 cut(s) 221, 361
BstHHI GCGC 2 cut(s) 363, 932
BstKTI GATC 3 cut(s) 426, 746, 889
BstMAI GTCTC 2 cut(s) 626, 819
BstMBI GATC 3 cut(s) 423, 743, 886
BstMCI CGRYCG 1 cut(s) 199
BstMWI GCNNNNNNNGC 2 cut(s) 367, 859
BstNSI RCATGY 1 cut(s) 143
BstUI CGCG 2 cut(s) 221, 361
BstV1I GCAGC 1 cut(s) 31
BstV2I GAAGAC 1 cut(s) 406
BstX2I RGATCY 1 cut(s) 886
BstYI RGATCY 1 cut(s) 886
BstZI CGGCCG 1 cut(s) 196
BsuRI GGCC 3 cut(s) 198, 298, 445
BtgI CCRYGG 1 cut(s) 168
BtsIMutI CAGTG 4 cut(s) 408, 523, 571, 891
BveI ACCTGC 1 cut(s) 361
Cac8I GCNNGC 4 cut(s) 141, 223, 439, 443
CfoI GCGC 2 cut(s) 363, 932
Cfr13I GGNCC 1 cut(s) 63
CseI GACGC 1 cut(s) 842
Csp6I GTAC 4 cut(s) 154, 569, 757, 823
CviAII CATG 3 cut(s) 140, 169, 927
CviQI GTAC 4 cut(s) 154, 569, 757, 823
DdeI CTNAG 4 cut(s) 21, 40, 389, 563
DpnI GATC 3 cut(s) 425, 745, 888
DpnII GATC 3 cut(s) 423, 743, 886
EaeI YGGCCR 2 cut(s) 196, 296
EagI CGGCCG 1 cut(s) 196
Eam1104I CTCTTC 1 cut(s) 390
EarI CTCTTC 1 cut(s) 390
EclXI CGGCCG 1 cut(s) 196
Eco130I CCWWGG 2 cut(s) 168, 720
Eco147I AGGCCT 1 cut(s) 445
Eco24I GRGCYC 1 cut(s) 98
Eco31I GGTCTC 2 cut(s) 626, 819
Eco32I GATATC 2 cut(s) 31, 481
Eco47I GGWCC 1 cut(s) 63
Eco52I CGGCCG 1 cut(s) 196
Eco57I CTGAAG 1 cut(s) 499
Eco88I CYCGRG 2 cut(s) 205, 816
EcoO109I RGGNCCY 1 cut(s) 63
EcoRV GATATC 2 cut(s) 31, 481
EcoT14I CCWWGG 2 cut(s) 168, 720
EcoT22I ATGCAT 1 cut(s) 141
EcoT38I GRGCYC 1 cut(s) 98
ErhI CCWWGG 2 cut(s) 168, 720
FaeI CATG 3 cut(s) 143, 172, 930
FalI AAGNNNNNCTT 2 cut(s) 430, 462
FaqI GGGAC 3 cut(s) 49, 119, 134
FatI CATG 3 cut(s) 139, 168, 926
FauNDI CATATG 1 cut(s) 428
FbaI TGATCA 1 cut(s) 423
Fnu4HI GCNGC 2 cut(s) 45, 199
FriOI GRGCYC 1 cut(s) 98
Fsp4HI GCNGC 2 cut(s) 45, 199
FspBI CTAG 1 cut(s) 839
GlaI GCGC 2 cut(s) 362, 931
GluI GCNGC 2 cut(s) 45, 199
HaeIII GGCC 3 cut(s) 198, 298, 445
HapII CCGG 3 cut(s) 255, 299, 811
HgaI GACGC 1 cut(s) 842
HhaI GCGC 2 cut(s) 363, 932
Hin1II CATG 3 cut(s) 143, 172, 930
Hin6I GCGC 2 cut(s) 361, 930
HinP1I GCGC 2 cut(s) 361, 930
HincII GTYRAC 1 cut(s) 792
HindII GTYRAC 1 cut(s) 792
HindIII AAGCTT 1 cut(s) 37
HinfI GANTC 4 cut(s) 23, 107, 173, 864
HpaII CCGG 3 cut(s) 255, 299, 811
HphI GGTGA 8 cut(s) 45, 168, 322, 512, 550, 556, 603, 889
Hpy166II GTNNAC 4 cut(s) 408, 569, 706, 792
Hpy188I TCNGA 5 cut(s) 22, 28, 511, 634, 692
Hpy188III TCNNGA 2 cut(s) 376, 816
Hpy8I GTNNAC 4 cut(s) 408, 569, 706, 792
Hpy99I CGWCG 1 cut(s) 858
HpyAV CCTTC 3 cut(s) 108, 317, 852
HpyCH4III ACNGT 3 cut(s) 412, 544, 895
HpyCH4IV ACGT 2 cut(s) 600, 825
HpyCH4V TGCA 8 cut(s) 7, 47, 91, 139, 370, 437, 712, 773
HpyF10VI GCNNNNNNNGC 2 cut(s) 367, 859
HpyF3I CTNAG 4 cut(s) 21, 40, 389, 563
HpySE526I ACGT 2 cut(s) 600, 825
Hsp92II CATG 3 cut(s) 143, 172, 930
HspAI GCGC 2 cut(s) 361, 930
Ksp22I TGATCA 1 cut(s) 423
Kzo9I GATC 3 cut(s) 423, 743, 886
LguI GCTCTTC 1 cut(s) 390
Lsp1109I GCAGC 1 cut(s) 31
LweI GCATC 1 cut(s) 56
MaeI CTAG 1 cut(s) 839
MaeII ACGT 2 cut(s) 600, 825
MaeIII GTNAC 5 cut(s) 174, 485, 538, 601, 895
MalI GATC 3 cut(s) 425, 745, 888
MboI GATC 3 cut(s) 423, 743, 886
MboII GAAGA 4 cut(s) 243, 377, 406, 673
MfeI CAATTG 1 cut(s) 648
MflI RGATCY 1 cut(s) 886
MhlI GDGCHC 1 cut(s) 98
MluCI AATT 4 cut(s) 585, 648, 783, 800
MlyI GAGTC 3 cut(s) 32, 101, 182
MmeI TCCRAC 1 cut(s) 551
Mph1103I ATGCAT 1 cut(s) 141
MseI TTAA 3 cut(s) 468, 557, 797
MslI CAYNNNNRTG 1 cut(s) 149
MspI CCGG 3 cut(s) 255, 299, 811
MunI CAATTG 1 cut(s) 648
Mva1269I GAATGC 1 cut(s) 594
MvnI CGCG 2 cut(s) 221, 361
MwoI GCNNNNNNNGC 2 cut(s) 367, 859
NcoI CCATGG 1 cut(s) 168
NdeI CATATG 1 cut(s) 428
NdeII GATC 3 cut(s) 423, 743, 886
NlaIII CATG 3 cut(s) 143, 172, 930
NlaIV GGNNCC 6 cut(s) 65, 75, 718, 726, 780, 888
NmeAIII GCCGAG 1 cut(s) 303
NmuCI GTSAC 3 cut(s) 174, 538, 895
NsiI ATGCAT 1 cut(s) 141
NspI RCATGY 1 cut(s) 143
PaeI GCATGC 1 cut(s) 143
PaeR7I CTCGAG 2 cut(s) 205, 816
PceI AGGCCT 1 cut(s) 445
PciSI GCTCTTC 1 cut(s) 390
PcsI WCGNNNNNNNCGW 1 cut(s) 597
PctI GAATGC 1 cut(s) 594
PfeI GAWTC 1 cut(s) 864
Pfl23II CGTACG 1 cut(s) 822
PkrI GCNGC 2 cut(s) 46, 200
PleI GAGTC 3 cut(s) 31, 101, 181
PpsI GAGTC 3 cut(s) 31, 101, 181
Ppu21I YACGTR 1 cut(s) 826
PpuMI RGGWCCY 1 cut(s) 63
Psp5II RGGWCCY 1 cut(s) 63
PspLI CGTACG 1 cut(s) 822
PspN4I GGNNCC 6 cut(s) 65, 75, 718, 726, 780, 888
PspPI GGNCC 1 cut(s) 63
PspPPI RGGWCCY 1 cut(s) 63
PspXI VCTCGAGB 1 cut(s) 205
PsuI RGATCY 1 cut(s) 886
RsaI GTAC 4 cut(s) 155, 570, 758, 824
RsaNI GTAC 4 cut(s) 154, 569, 757, 823
RseI CAYNNNNRTG 1 cut(s) 149
SapI GCTCTTC 1 cut(s) 390
SaqAI TTAA 3 cut(s) 468, 557, 797
SatI GCNGC 2 cut(s) 45, 199
Sau3AI GATC 3 cut(s) 423, 743, 886
Sau96I GGNCC 1 cut(s) 63
ScaI AGTACT 1 cut(s) 758
SchI GAGTC 3 cut(s) 32, 101, 182
SduI GDGCHC 1 cut(s) 98
SfaNI GCATC 1 cut(s) 56
Sfr274I CTCGAG 2 cut(s) 205, 816
SinI GGWCC 1 cut(s) 63
SlaI CTCGAG 2 cut(s) 205, 816
SmiMI CAYNNNNRTG 1 cut(s) 149
SmlI CTYRAG 2 cut(s) 205, 816
SmoI CTYRAG 2 cut(s) 205, 816
SphI GCATGC 1 cut(s) 143
Sse9I AATT 4 cut(s) 585, 648, 783, 800
SseBI AGGCCT 1 cut(s) 445
SsiI CCGC 4 cut(s) 199, 219, 267, 641
SspI AATATT 1 cut(s) 668
SspMI CTAG 1 cut(s) 839
StuI AGGCCT 1 cut(s) 445
StyI CCWWGG 2 cut(s) 168, 720
TaaI ACNGT 3 cut(s) 412, 544, 895
TaiI ACGT 2 cut(s) 603, 828
TaqI TCGA 3 cut(s) 206, 817, 856
TaqII GACCGA 1 cut(s) 608
TasI AATT 4 cut(s) 585, 648, 783, 800
TatI WGTACW 2 cut(s) 153, 756
TauI GCSGC 1 cut(s) 201
TfiI GAWTC 1 cut(s) 864
Tru1I TTAA 3 cut(s) 468, 557, 797
Tru9I TTAA 3 cut(s) 468, 557, 797
TscAI CASTG 4 cut(s) 415, 523, 571, 898
TseFI GTSAC 3 cut(s) 174, 538, 895
TseI GCWGC 1 cut(s) 44
Tsp45I GTSAC 3 cut(s) 174, 538, 895
TspDTI ATGAA 1 cut(s) 915
TspGWI ACGGA 1 cut(s) 777
TspRI CASTG 4 cut(s) 415, 523, 571, 898
VpaK11BI GGWCC 1 cut(s) 63
XapI RAATTY 1 cut(s) 800
XceI RCATGY 1 cut(s) 143
XhoI CTCGAG 2 cut(s) 205, 816
XspI CTAG 1 cut(s) 839
ZrmI AGTACT 1 cut(s) 758
Zsp2I ATGCAT 1 cut(s) 141
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.