Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr09

Orthogroup ID

OG0002964

Physical Location & Seq

Genomic Coordinates Forward (+)

32054232 .. 32058147

Gene Structure 3916 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

MD09G1250400.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1392 bp

ATGAGAAAGCAGGATCTTATTGCATCTCTTCTTTCTTTGTACATAGATGAGTCATGCACTTTACTGCCTAATATGGTGTTGGATCGAACAGGTGTTTGTGTTAGGATATCCTCAAAAGCGGAGGCCCTCATCTGGCGTGCTGAGAGACTTTTCTTCCTGAATGGAGAACAGGATTTGTCAGCATTTCTACTAGTGGATTTGGGAATAGTTAAGTATCCAACTTACAAATGCACAGTATCACAACAAATTTTCTCAGGTCGAGATGGCTTGCTTGCTTATGAAGAGGCCATTGAAGTGGCACAGATAATTGATCAAGCTCTTGATGGAAGCAACAATGGAATTGTGTTGAAATGCATTAATATAGCTGATGCTCGTATGTCTAGTGAAGTAACTCAATTCTTAACTTCTGAATCAATGGCTCCATTTCTGTCCTGTTTCTCTGCTCCGCATGTCTACTCAAAAGTGGTTTTACTAGGAATCTCCTTTCTAGAGCGTGAGCATAGGTATAGTGATGCAATATATTTACTGAAGCGGCTGCTAAAGTGCTTCACTTGTGATGGTAGAAGAGGATACTGGACACTAAGGTTATCAATTGACTTAGAGCAAATGGGCTATCTGAATGAGAGCCTTTCAGTTGCCGAAAATGGGTTACTAGATCAATGGGTACGTGCTGGTTCAAGAGTGGCACTTCAAAGGAGAGTCCTTCGCTTAGGAAAACCACCAAGGCGATGGAAAACACCTAGTTTTGCAGAGTCTGTCAAGAGGAAGATTGTCGAGGTTCATGTACAAGGGAGACCTCTAAATTCGGTGATTGGTATGAAGAGCAGGTTTTATGGAGAAGATGGAGAGCAATGTGGGGTAGAGCAGCTAGCATTGCAGCATTATGCTGGGGAAGGGTGGCAGGGTGTTCATTCAGAAAGTGGCATTTGGTTGACTATTTTTTGGGCTTCTTATATTGCTCCCTTGGATTTGCAAACTGATAGCTTTTATCTGGTGAGAAAGAGCCATATGGAATCTCACTTACAGAAAATTCATGATGGCATGGCTGAGGAGATCCTCATCACTTCATGGGAGTTACACGAAGGAACATCATGCAGAGGAGTTAATTGGGACCGTCATTCCCTGTCCGAGCTTCGAGCAGCTGTTACGTGCATTGGTGGCCCTTGTTTGGCCTCATTCTGTCGACATCTAGCACAAGATTATCGCAGCTGGTCAAGCGGAATGCCAGATTTACTGCTGTGGCGCTTCCATGGGAAATACAAAGGTGAAGCCAAGCTTGTCGAAGTTAAAGGCCCCAGGGATAGACTCTCTGAACAGCAGCGAGCATGGCTGCTGCTTTTCATGGACTGTGGGTTCAACGCTGAGGTCTGTAAAGTGATCCCTGTCTTGTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000003 GO:0000280 GO:0003674 GO:0003676 GO:0003677 GO:0003824 GO:0004518 GO:0004519 GO:0004520 GO:0004527 GO:0004536 GO:0005488 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0006139 GO:0006259 GO:0006281 GO:0006725 GO:0006807 GO:0006950 GO:0006974 GO:0006996 GO:0007049 GO:0007059 GO:0007127 GO:0007129 GO:0008150 GO:0008152 GO:0008409 GO:0009987 GO:0016043 GO:0016787 GO:0016788 GO:0016888 GO:0016893 GO:0017108 GO:0022402 GO:0022414 GO:0033554 GO:0034641 GO:0036297 GO:0043170 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044237 GO:0044238 GO:0044260 GO:0044424 GO:0044464 GO:0045132 GO:0045143 GO:0046483 GO:0048256 GO:0048285 GO:0050896 GO:0051276 GO:0051321 GO:0051716 GO:0061982 GO:0070192 GO:0070336 GO:0071704 GO:0071840 GO:0090304 GO:0090305 GO:0097159 GO:0098813 GO:0140013 GO:0140097 GO:1901360 GO:1901363 GO:1903046

KEGG Pathways

Metabolic & Signaling

ko03460 map03460

Pfam Domains

Protein Families

HIRAN VRR_NUC

Protein Analysis

464

Amino Acids

52.51

Weight (kDa)

7.15

Isoelectric Point (pI)

42.08

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
FAN1_TPR	PF21170	129 - 237	2.4e-13	Fanconi-associated nuclease 1, TPR domain
VRR_NUC	PF08774	346 - 459	9e-37	VRR-NUC domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

Style Settings

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0002964)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT1G48360 AT1G48360 AT1G48360 AT1G48360 AT1G48360 AT1G48360
fragaria_vesca	FvH4_4g12370 FvH4_4g12370 FvH4_4g12370 FvH4_4g12370 FvH4_4g12370 FvH4_4g12370 FvH4_4g12370 FvH4_4g12370
malus_domestica	MD00G1163900.v1.1 MD09G1250200.v1.1 MD09G1250400.v1.1
prunus_persica	Prupe.1G176700_v2.0.a1 Prupe.1G176700_v2.0.a1 Prupe.1G176700_v2.0.a1 Prupe.1G176700_v2.0.a1 Prupe.1G176700_v2.0.a1
pyrus_communis	pycom04g02280 pycom13g29380
rosa_chinensis	RchiOBHm_Chr4g0410481
rosa_laevigata	RLG00000008424
rosa_multiflora	Rmu_sc0001969.1_g000008 Rmu_sc0016181.1_g000004
rosa_roxburghii	Rroxscaffold_5G00355290
rosa_rugosa	Rorug04G0114400
rosa_samantha	Rh4AG160900 Rh4BG160300 Rh4CG173100 Rh4DG156400
rosa_wichuraiana	Rw0G014390 Rw0G014400 Rw4G013420 Rw4G037760

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
Acc36I	ACCTGC	1 cut(s)	816
AccI	GTMKAC	2 cut(s)	453, 1183
AciI	CCGC	4 cut(s)	119, 446, 532, 1218
AclWI	GGATC	4 cut(s)	21, 90, 1048, 1372
AcsI	RAATTY	3 cut(s)	246, 802, 1029
AcuI	CTGAAG	1 cut(s)	548
AfaI	GTAC	3 cut(s)	41, 666, 786
AfiI	CCNNNNNNNGG	3 cut(s)	132, 645, 1168
AgsI	TTSAA	5 cut(s)	293, 349, 678, 692, 1357
AhlI	ACTAGT	1 cut(s)	190
AjnI	CCWGG	1 cut(s)	1295
AloI	GAACNNNNNNTCC	2 cut(s)	1337, 1369
AluBI	AGCT	8 cut(s)	317, 365, 868, 984, 1132, 1142, 1209, 1276
AluI	AGCT	8 cut(s)	317, 365, 868, 984, 1132, 1142, 1209, 1276
Alw26I	GTCTC	2 cut(s)	139, 787
AlwI	GGATC	4 cut(s)	21, 90, 1048, 1372
AlwNI	CAGNNNCTG	1 cut(s)	755
AoxI	GGCC	5 cut(s)	123, 285, 1159, 1170, 1291
ApeKI	GCWGC	8 cut(s)	535, 865, 877, 1139, 1206, 1318, 1330, 1333
ApoI	RAATTY	3 cut(s)	246, 802, 1029
AseI	ATTAAT	1 cut(s)	357
AspLEI	GCGC	1 cut(s)	1245
AspS9I	GGNCC	4 cut(s)	124, 1111, 1160, 1292
AsuHPI	GGTGA	3 cut(s)	820, 1006, 1277
AsuNHI	GCTAGC	1 cut(s)	868
AvaII	GGWCC	1 cut(s)	1111
BbvCI	CCTCAGC	2 cut(s)	1047, 1362
BbvI	GCAGC	8 cut(s)	522, 877, 889, 1151, 1218, 1317, 1320, 1330
BccI	CCATC	6 cut(s)	257, 317, 551, 723, 836, 1031
BciT130I	CCWGG	1 cut(s)	1297
BciVI	GTATCC	2 cut(s)	225, 563
BclI	TGATCA	1 cut(s)	310
BcoDI	GTCTC	2 cut(s)	139, 787
BcuI	ACTAGT	1 cut(s)	190
BfaI	CTAG	8 cut(s)	191, 381, 473, 488, 653, 741, 869, 1190
BfoI	RGCGCY	1 cut(s)	1246
BfuAI	ACCTGC	1 cut(s)	816
BfuI	GTATCC	2 cut(s)	225, 563
BisI	GCNGC	9 cut(s)	533, 536, 866, 878, 1140, 1207, 1319, 1331, 1334
BlsI	GCNGC	9 cut(s)	534, 537, 867, 879, 1141, 1208, 1320, 1332, 1335
Bme1390I	CCNGG	1 cut(s)	1297
Bme18I	GGWCC	1 cut(s)	1111
BmgT120I	GGNCC	4 cut(s)	124, 1111, 1160, 1292
BmiI	GGNNCC	3 cut(s)	420, 1112, 1294
BmrFI	CCNGG	1 cut(s)	1297
BmsI	GCATC	3 cut(s)	32, 358, 502
BmtI	GCTAGC	1 cut(s)	872
Bpu10I	CCTNAGC	3 cut(s)	709, 1047, 1362
BsaAI	YACGTR	2 cut(s)	668, 1149
BsaBI	GATNNNNATC	1 cut(s)	1058
BsaI	GGTCTC	1 cut(s)	787
BsaJI	CCNNGG	5 cut(s)	722, 963, 1249, 1295, 1296
Bsc4I	CCNNNNNNNGG	3 cut(s)	132, 645, 1168
Bse1I	ACTGG	1 cut(s)	578
Bse3DI	GCAATG	2 cut(s)	857, 872
Bse8I	GATNNNNATC	1 cut(s)	1058
BseBI	CCWGG	1 cut(s)	1297
BseDI	CCNNGG	5 cut(s)	722, 963, 1249, 1295, 1296
BseJI	GATNNNNATC	1 cut(s)	1058
BseLI	CCNNNNNNNGG	3 cut(s)	132, 645, 1168
BseMI	GCAATG	2 cut(s)	857, 872
BseMII	CTCAG	4 cut(s)	132, 267, 1038, 1353
BseNI	ACTGG	1 cut(s)	578
BseRI	GAGGAG	2 cut(s)	1064, 1113
BseXI	GCAGC	8 cut(s)	522, 877, 889, 1151, 1218, 1317, 1320, 1330
BseYI	CCCAGC	1 cut(s)	887
BshFI	GGCC	5 cut(s)	125, 287, 1161, 1172, 1293
BslFI	GGGAC	1 cut(s)	1124
BslI	CCNNNNNNNGG	3 cut(s)	132, 645, 1168
BsmAI	GTCTC	2 cut(s)	139, 787
BsmFI	GGGAC	1 cut(s)	1124
BsmI	GAATGC	1 cut(s)	1227
BsnI	GGCC	5 cut(s)	125, 287, 1161, 1172, 1293
Bso31I	GGTCTC	1 cut(s)	787
Bsp1407I	TGTACA	2 cut(s)	39, 784
Bsp143I	GATC	6 cut(s)	13, 82, 310, 655, 1053, 1377
Bsp19I	CCATGG	1 cut(s)	1249
BspACI	CCGC	4 cut(s)	119, 446, 532, 1218
BspANI	GGCC	5 cut(s)	125, 287, 1161, 1172, 1293
BspCNI	CTCAG	4 cut(s)	133, 266, 1039, 1354
BspHI	TCATGA	1 cut(s)	1033
BspLI	GGNNCC	3 cut(s)	420, 1112, 1294
BspMI	ACCTGC	1 cut(s)	816
BspOI	GCTAGC	1 cut(s)	872
BspPI	GGATC	4 cut(s)	21, 90, 1048, 1372
BspQI	GCTCTTC	1 cut(s)	815
BspTNI	GGTCTC	1 cut(s)	787
BsrDI	GCAATG	2 cut(s)	857, 872
BsrGI	TGTACA	2 cut(s)	39, 784
BsrI	ACTGG	1 cut(s)	578
BssECI	CCNNGG	5 cut(s)	722, 963, 1249, 1295, 1296
BssMI	GATC	6 cut(s)	13, 82, 310, 655, 1053, 1377
BssT1I	CCWWGG	3 cut(s)	722, 963, 1249
Bst2UI	CCWGG	1 cut(s)	1297
Bst4CI	ACNGT	3 cut(s)	235, 1115, 1349
Bst6I	CTCTTC	4 cut(s)	33, 276, 559, 815
BstAUI	TGTACA	2 cut(s)	39, 784
BstBAI	YACGTR	2 cut(s)	668, 1149
BstC8I	GCNNGC	5 cut(s)	138, 269, 273, 870, 1323
BstDEI	CTNAG	7 cut(s)	141, 253, 581, 598, 709, 1047, 1362
BstDSI	CCRYGG	1 cut(s)	1249
BstH2I	RGCGCY	1 cut(s)	1246
BstHHI	GCGC	1 cut(s)	1245
BstKTI	GATC	6 cut(s)	16, 85, 313, 658, 1056, 1380
BstMAI	GTCTC	2 cut(s)	139, 787
BstMBI	GATC	6 cut(s)	13, 82, 310, 655, 1053, 1377
BstMWI	GCNNNNNNNGC	4 cut(s)	874, 1158, 1215, 1327
BstNI	CCWGG	1 cut(s)	1297
BstNSI	RCATGY	1 cut(s)	452
BstSCI	CCNGG	1 cut(s)	1295
BstV1I	GCAGC	8 cut(s)	522, 877, 889, 1151, 1218, 1317, 1320, 1330
BstX2I	RGATCY	2 cut(s)	13, 1053
BstXI	CCANNNNNNTGG	2 cut(s)	295, 729
BstYI	RGATCY	2 cut(s)	13, 1053
BsuI	GTATCC	2 cut(s)	225, 563
BsuRI	GGCC	5 cut(s)	125, 287, 1161, 1172, 1293
BtgI	CCRYGG	1 cut(s)	1249
BtgZI	GCGATG	1 cut(s)	742
BveI	ACCTGC	1 cut(s)	816
Cac8I	GCNNGC	5 cut(s)	138, 269, 273, 870, 1323
CaiI	CAGNNNCTG	1 cut(s)	755
CciI	TCATGA	1 cut(s)	1033
CfoI	GCGC	1 cut(s)	1245
Cfr13I	GGNCC	4 cut(s)	124, 1111, 1160, 1292
Csp6I	GTAC	3 cut(s)	40, 665, 785
CviQI	GTAC	3 cut(s)	40, 665, 785
DdeI	CTNAG	7 cut(s)	141, 253, 581, 598, 709, 1047, 1362
DpnI	GATC	6 cut(s)	15, 84, 312, 657, 1055, 1379
DpnII	GATC	6 cut(s)	13, 82, 310, 655, 1053, 1377
Eam1104I	CTCTTC	4 cut(s)	33, 276, 559, 815
EarI	CTCTTC	4 cut(s)	33, 276, 559, 815
Eco130I	CCWWGG	3 cut(s)	722, 963, 1249
Eco31I	GGTCTC	1 cut(s)	787
Eco32I	GATATC	1 cut(s)	108
Eco47I	GGWCC	1 cut(s)	1111
Eco57I	CTGAAG	1 cut(s)	548
EcoO109I	RGGNCCY	2 cut(s)	124, 1292
EcoRII	CCWGG	1 cut(s)	1295
EcoRV	GATATC	1 cut(s)	108
EcoT14I	CCWWGG	3 cut(s)	722, 963, 1249
EcoT22I	ATGCAT	1 cut(s)	356
ErhI	CCWWGG	3 cut(s)	722, 963, 1249
FalI	AAGNNNNNCTT	2 cut(s)	1260, 1292
FaqI	GGGAC	1 cut(s)	1124
FauNDI	CATATG	1 cut(s)	1008
FbaI	TGATCA	1 cut(s)	310
FblI	GTMKAC	2 cut(s)	453, 1183
Fnu4HI	GCNGC	9 cut(s)	533, 536, 866, 878, 1140, 1207, 1319, 1331, 1334
Fsp4HI	GCNGC	9 cut(s)	533, 536, 866, 878, 1140, 1207, 1319, 1331, 1334
FspBI	CTAG	8 cut(s)	191, 381, 473, 488, 653, 741, 869, 1190
GlaI	GCGC	1 cut(s)	1244
GluI	GCNGC	9 cut(s)	533, 536, 866, 878, 1140, 1207, 1319, 1331, 1334
GsaI	CCCAGC	1 cut(s)	891
HaeII	RGCGCY	1 cut(s)	1246
HaeIII	GGCC	5 cut(s)	125, 287, 1161, 1172, 1293
HhaI	GCGC	1 cut(s)	1245
Hin6I	GCGC	1 cut(s)	1243
HinP1I	GCGC	1 cut(s)	1243
HincII	GTYRAC	2 cut(s)	933, 1184
HindII	GTYRAC	2 cut(s)	933, 1184
HindIII	AAGCTT	1 cut(s)	1274
HinfI	GANTC	7 cut(s)	50, 410, 477, 699, 752, 1013, 1305
HphI	GGTGA	3 cut(s)	820, 1006, 1277
Hpy166II	GTNNAC	3 cut(s)	454, 933, 1184
Hpy188I	TCNGA	5 cut(s)	409, 618, 916, 1129, 1312
Hpy188III	TCNNGA	7 cut(s)	157, 260, 320, 488, 678, 760, 1034
Hpy8I	GTNNAC	3 cut(s)	454, 933, 1184
HpyAV	CCTTC	3 cut(s)	713, 887, 1076
HpyCH4III	ACNGT	3 cut(s)	235, 1115, 1349
HpyCH4IV	ACGT	2 cut(s)	667, 1148
HpyF10VI	GCNNNNNNNGC	4 cut(s)	874, 1158, 1215, 1327
HpyF3I	CTNAG	7 cut(s)	141, 253, 581, 598, 709, 1047, 1362
HpySE526I	ACGT	2 cut(s)	667, 1148
HspAI	GCGC	1 cut(s)	1243
Ksp22I	TGATCA	1 cut(s)	310
Kzo9I	GATC	6 cut(s)	13, 82, 310, 655, 1053, 1377
LguI	GCTCTTC	1 cut(s)	815
LmnI	GCTCC	3 cut(s)	424, 448, 964
Lsp1109I	GCAGC	8 cut(s)	522, 877, 889, 1151, 1218, 1317, 1320, 1330
LweI	GCATC	3 cut(s)	32, 358, 502
MaeI	CTAG	8 cut(s)	191, 381, 473, 488, 653, 741, 869, 1190
MaeII	ACGT	2 cut(s)	667, 1148
MaeIII	GTNAC	4 cut(s)	388, 648, 1074, 1144
MalI	GATC	6 cut(s)	15, 84, 312, 657, 1055, 1379
MboI	GATC	6 cut(s)	13, 82, 310, 655, 1053, 1377
MboII	GAAGA	7 cut(s)	20, 145, 293, 576, 778, 832, 851
MfeI	CAATTG	1 cut(s)	591
MflI	RGATCY	2 cut(s)	13, 1053
MluCI	AATT	8 cut(s)	246, 306, 339, 395, 591, 802, 1029, 1105
MlyI	GAGTC	4 cut(s)	59, 708, 761, 1299
MmeI	TCCRAC	2 cut(s)	60, 242
Mph1103I	ATGCAT	1 cut(s)	356
MseI	TTAA	5 cut(s)	210, 357, 401, 1104, 1287
MslI	CAYNNNNRTG	1 cut(s)	293
MspA1I	CMGCKG	2 cut(s)	1142, 1209
MspR9I	CCNGG	1 cut(s)	1297
MunI	CAATTG	1 cut(s)	591
Mva1269I	GAATGC	1 cut(s)	1227
MvaI	CCWGG	1 cut(s)	1297
MwoI	GCNNNNNNNGC	4 cut(s)	874, 1158, 1215, 1327
NcoI	CCATGG	1 cut(s)	1249
NdeI	CATATG	1 cut(s)	1008
NdeII	GATC	6 cut(s)	13, 82, 310, 655, 1053, 1377
NheI	GCTAGC	1 cut(s)	868
NlaIV	GGNNCC	3 cut(s)	420, 1112, 1294
NsiI	ATGCAT	1 cut(s)	356
NspI	RCATGY	1 cut(s)	452
PagI	TCATGA	1 cut(s)	1033
PasI	CCCWGGG	1 cut(s)	1296
PciSI	GCTCTTC	1 cut(s)	815
PctI	GAATGC	1 cut(s)	1227
PfeI	GAWTC	3 cut(s)	410, 477, 1013
PkrI	GCNGC	9 cut(s)	534, 537, 867, 879, 1141, 1208, 1320, 1332, 1335
PleI	GAGTC	4 cut(s)	58, 707, 760, 1299
PpsI	GAGTC	4 cut(s)	58, 707, 760, 1299
Ppu21I	YACGTR	2 cut(s)	668, 1149
PshBI	ATTAAT	1 cut(s)	357
Psp6I	CCWGG	1 cut(s)	1295
PspFI	CCCAGC	1 cut(s)	887
PspGI	CCWGG	1 cut(s)	1295
PspN4I	GGNNCC	3 cut(s)	420, 1112, 1294
PspPI	GGNCC	4 cut(s)	124, 1111, 1160, 1292
PstNI	CAGNNNCTG	1 cut(s)	755
PsuI	RGATCY	2 cut(s)	13, 1053
PvuII	CAGCTG	2 cut(s)	1142, 1209
RsaI	GTAC	3 cut(s)	41, 666, 786
RsaNI	GTAC	3 cut(s)	40, 665, 785
RseI	CAYNNNNRTG	1 cut(s)	293
SalI	GTCGAC	1 cut(s)	1182
SapI	GCTCTTC	1 cut(s)	815
SaqAI	TTAA	5 cut(s)	210, 357, 401, 1104, 1287
SatI	GCNGC	9 cut(s)	533, 536, 866, 878, 1140, 1207, 1319, 1331, 1334
Sau3AI	GATC	6 cut(s)	13, 82, 310, 655, 1053, 1377
Sau96I	GGNCC	4 cut(s)	124, 1111, 1160, 1292
SchI	GAGTC	4 cut(s)	59, 708, 761, 1299
ScrFI	CCNGG	1 cut(s)	1297
SfaNI	GCATC	3 cut(s)	32, 358, 502
SinI	GGWCC	1 cut(s)	1111
SmiMI	CAYNNNNRTG	1 cut(s)	293
SpeI	ACTAGT	1 cut(s)	190
Sse9I	AATT	8 cut(s)	246, 306, 339, 395, 591, 802, 1029, 1105
SsiI	CCGC	4 cut(s)	119, 446, 532, 1218
SspMI	CTAG	8 cut(s)	191, 381, 473, 488, 653, 741, 869, 1190
StyD4I	CCNGG	1 cut(s)	1295
StyI	CCWWGG	3 cut(s)	722, 963, 1249
TaaI	ACNGT	3 cut(s)	235, 1115, 1349
TaiI	ACGT	2 cut(s)	670, 1151
TaqI	TCGA	6 cut(s)	85, 259, 774, 1135, 1183, 1281
TasI	AATT	8 cut(s)	246, 306, 339, 395, 591, 802, 1029, 1105
TatI	WGTACW	2 cut(s)	39, 784
TauI	GCSGC	1 cut(s)	535
TfiI	GAWTC	3 cut(s)	410, 477, 1013
Tru1I	TTAA	5 cut(s)	210, 357, 401, 1104, 1287
Tru9I	TTAA	5 cut(s)	210, 357, 401, 1104, 1287
TseI	GCWGC	8 cut(s)	535, 865, 877, 1139, 1206, 1318, 1330, 1333
TspDTI	ATGAA	7 cut(s)	294, 770, 833, 899, 1022, 1056, 1330
VpaK11BI	GGWCC	1 cut(s)	1111
VspI	ATTAAT	1 cut(s)	357
XapI	RAATTY	3 cut(s)	246, 802, 1029
XbaI	TCTAGA	1 cut(s)	487
XceI	RCATGY	1 cut(s)	452
XcmI	CCANNNNNNNNNTGG	1 cut(s)	726
XmiI	GTMKAC	2 cut(s)	453, 1183
XspI	CTAG	8 cut(s)	191, 381, 473, 488, 653, 741, 869, 1190
Zsp2I	ATGCAT	1 cut(s)	356

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.