Basic Information

Type: gene

Biological Identity

Species

rosa_samantha

Chromosome

Chr4D

Orthogroup ID

OG0002964

Physical Location & Seq

Genomic Coordinates Reverse (-)

31289758 .. 31298886

Gene Structure 9129 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

Rh4DG156400.1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2301 bp

ATGATTTATTTCATGATGAGAGTCATGTACAAGATGCGTCGTGGGGGTTGTCTAACTCGAGGGCTGTTGACGATAGTCAAAATCAGGTTGACAGTCCATCCAGAGAGCCAATCAATAAGGACGAGATCAGTGAGGTTTGGTGATCAAACTGATTTATGTCTTGGTGCAAGCATTTATCTCTCAAGAGATCCTGATAATGTTAAGGATCCTAATGCTATCAAGGTTGTTTATTCAGATTCGGGATGCCTTAAAATGCTTGGCTTTCTCCCTCGCAAGCTAGCTGAGTATTTGTCTCCTCTGATAGACCAGTATAGTCTAAATTTTGAGGGATGCGTTACCTCTGTTCCTAAGCGTTCTATTGATGTTGTCCCAATACAGATAGTGTGTCACAAGACCAGTGATAATAAGGACTTTAATGATGACAAGGTTTTCAAATGTTTATGGAGAAATGCTCTGCAAGTAATTGAATCTACAAAGTCCTGTCCGCCCAGTGCAATAAAATATCAGGATAACTTTTGTGTTTTCATACGTGAGGTTTTGAGAAACAGTTCTCACCTCTTGACAAATGATGAAAAGAATTTCATCAAGTCATTTACTTCGATGTCAGATGATAGTCAGAGGCTATTTGTTCGACTTTATACACGGAAAGGCCCATGGTTTCGGCTGTCTGCTATTTCATACCCAGAAGTACTGGATCCCCAACAAGCAGTGAAAGAGCTATCTGAAACAGGGTATGCATCTTGCTTTGAAATTACAAATAAACTATCTGATGATTGTATTAAGGAGATTTTGGATTTGCTCACTGTATCTGAGCTACGTGAAATCTTGTGCATCCTTAAGCAGAGTTGCAAACGTGGCCTAAGAAAGCAGGATCTCATTGCATCTCTTATATCTTCTTACGATGAAGATTTGTGCCCATTACTACCTAGTATGGTTTTGCACAGAACAGGATCCTGTGTTAGGATATCTTCAAATGCGGAGTCCCTTATCTGGCGTGCTGAGAGGCTTTTCTTCCTGAATGGTGAACAGGATCTCTCGGCATTTCTACTGGTGGATTTGGGAATAGTCAAGTATCCAACTTACAAGTGCATAGTCTCACAACAAATTTTCTCAGCTCGAGATGATTTACTTGGCTATGAAGAGGCTATTGAAGTGGCACAAATAGTTGATGAAGCTCTTGATGGAAGCAACACTGGATTGGTCTTAAAGTGCATAAAGATAGCTGATTCCCATTTATCCAATCCGGTTCAATCCTTGACCTCTGAATCAATGGCTCCATTCCTTTCCCACTTTTCAGCTACATTTGTCTACTCAAAAGTGGTTTTACTAGGAATTTCTTTTCTTGAGCGTGAGCACAGGTACAATGATGCTATCTATTTACTGAAAAGGCTGCTGAATTGTTTCGCTCGTGATGGTAGAAGAGGATACTGGACACTACGGTTATCAATTGATTTGGAGCACATGGGCTATCTGAATGAGAGCCTTTTGGTTGCTGAAAATGGGTTACTAGATGAATGGGTGCGTGCTGGTTCAAGAGTGGCACTTCAAAGGAGAGTCCTTCGCTTGGGAAAACCACCGAGGCGATGGAAAACTCCTAGTTTTGCAGAGTCTGTCAAGAGGAAGATCATGGTGGTAAATGTTCCAGGGAGACCTTTAAATTGTGCAATTGGCACAAAGAGCAGGTTTTATGGAGAAGATGGGGAGCAATGCGGTGTAGAGCAGCTAGCTCTGCAATATTATGCCGAGGAAGGTGGATGGCACGGTGTTCATACAGAGAGTGGCATTTGGTTGACTATTTTTGGACTTCTTATGTGGGATATTATATTTTCCAATGTACCAAATGTCTTCCGCACCAGATTTCAGACCGCTCCCTTGGATTTAGAGACTGATAGCTTTTATCTAGTGAGAAAGAACCATATCGAGTCTCACCTACAGAAAATTCTTGATGGCATGGCTGAAGAGATTCTCATCACTTCATGGGAGTTACATGAGGGAACAGCATGTCGAGGAGTTAACTGGGGGCGACATTCCCTCTCTGAGCTTCGGGCAGCTGTTACATGCATCGGTGGCCCATGTGTGGCTGCATTTTGTCGACATCTTGCACAAGATTATCGTAGTTGGTCAAGTGGAATGCCAGATTTACTTCTGTGGCGCTTCCATGGGAAATACAGAGGTGAAGCGAAGCTTGTTGAAGTTAAAGGCCCCAGGGATAGACTCTCTGAACAGCAGCGAGCATGGCTATTGCTTTTAATGGATTGTGGGTTCAATGCCGAGGTCTGTAAAGTGAGCCCTATTGTCTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000003 GO:0000280 GO:0003674 GO:0003676 GO:0003677 GO:0003824 GO:0004518 GO:0004519 GO:0004520 GO:0004527 GO:0004536 GO:0005488 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0006139 GO:0006259 GO:0006281 GO:0006725 GO:0006807 GO:0006950 GO:0006974 GO:0006996 GO:0007049 GO:0007059 GO:0007127 GO:0007129 GO:0008150 GO:0008152 GO:0008409 GO:0009987 GO:0016043 GO:0016787 GO:0016788 GO:0016888 GO:0016893 GO:0017108 GO:0022402 GO:0022414 GO:0033554 GO:0034641 GO:0036297 GO:0043170 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044237 GO:0044238 GO:0044260 GO:0044424 GO:0044464 GO:0045132 GO:0045143 GO:0046483 GO:0048256 GO:0048285 GO:0050896 GO:0051276 GO:0051321 GO:0051716 GO:0061982 GO:0070192 GO:0070336 GO:0071704 GO:0071840 GO:0090304 GO:0090305 GO:0097159 GO:0098813 GO:0140013 GO:0140097 GO:1901360 GO:1901363 GO:1903046

KEGG Pathways

Metabolic & Signaling

ko03460 map03460

Pfam Domains

Protein Families

HIRAN VRR_NUC

Protein Analysis

766

Amino Acids

87.21

Weight (kDa)

8.27

Isoelectric Point (pI)

44.3

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
HIRAN	PF08797	49 - 118	8e-13	HIRAN domain
FAN1_HTH	PF21315	168 - 246	1.2e-17	FAN1, HTH domain
FAN1_TPR	PF21170	375 - 522	2.1e-13	Fanconi-associated nuclease 1, TPR domain
VRR_NUC	PF08774	650 - 763	2.4e-36	VRR-NUC domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0002964)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT1G48360 AT1G48360 AT1G48360 AT1G48360 AT1G48360 AT1G48360
fragaria_vesca	FvH4_4g12370 FvH4_4g12370 FvH4_4g12370 FvH4_4g12370 FvH4_4g12370 FvH4_4g12370 FvH4_4g12370 FvH4_4g12370
malus_domestica	MD00G1163900.v1.1 MD09G1250200.v1.1 MD09G1250400.v1.1
prunus_persica	Prupe.1G176700_v2.0.a1 Prupe.1G176700_v2.0.a1 Prupe.1G176700_v2.0.a1 Prupe.1G176700_v2.0.a1 Prupe.1G176700_v2.0.a1
pyrus_communis	pycom04g02280 pycom13g29380
rosa_chinensis	RchiOBHm_Chr4g0410481
rosa_laevigata	RLG00000008424
rosa_multiflora	Rmu_sc0001969.1_g000008 Rmu_sc0016181.1_g000004
rosa_roxburghii	Rroxscaffold_5G00355290
rosa_rugosa	Rorug04G0114400
rosa_samantha	Rh4AG160900 Rh4BG160300 Rh4CG173100 Rh4DG156400
rosa_wichuraiana	Rw0G014390 Rw0G014400 Rw4G013420 Rw4G037760

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
Acc36I	ACCTGC	1 cut(s)	1671
AccBSI	CCGCTC	1 cut(s)	1868
AccI	GTMKAC	2 cut(s)	1308, 2092
AciI	CCGC	5 cut(s)	485, 977, 1710, 1849, 1866
AclWI	GGATC	9 cut(s)	182, 200, 213, 689, 702, 879, 945, 958, 1038
AcsI	RAATTY	5 cut(s)	319, 577, 1104, 1332, 1938
AcuI	CTGAAG	1 cut(s)	1977
AfaI	GTAC	4 cut(s)	29, 690, 1361, 1836
AfiI	CCNNNNNNNGG	4 cut(s)	960, 990, 1564, 2077
AflII	CTTAAG	1 cut(s)	836
AjnI	CCWGG	2 cut(s)	1642, 2204
AloI	GAACNNNNNNTCC	2 cut(s)	2246, 2278
Alw21I	GWGCWC	2 cut(s)	1356, 1461
Alw26I	GTCTC	5 cut(s)	297, 1099, 1642, 1877, 1929
AlwI	GGATC	9 cut(s)	182, 200, 213, 689, 702, 879, 945, 958, 1038
AlwNI	CAGNNNCTG	1 cut(s)	1610
Ama87I	CYCGRG	2 cut(s)	57, 1116
AoxI	GGCC	4 cut(s)	649, 856, 2068, 2200
ApeKI	GCWGC	5 cut(s)	1390, 1720, 2048, 2081, 2227
ApoI	RAATTY	5 cut(s)	319, 577, 1104, 1332, 1938
ArsI	GACNNNNNNTTYG	2 cut(s)	965, 997
Asp700I	GAANNNNTTC	3 cut(s)	547, 1400, 1962
AspLEI	GCGC	1 cut(s)	2154
AspS9I	GGNCC	3 cut(s)	650, 2069, 2201
AsuHPI	GGTGA	5 cut(s)	152, 545, 1034, 1919, 2186
AsuNHI	GCTAGC	2 cut(s)	277, 1723
AvaI	CYCGRG	2 cut(s)	57, 1116
BaeGI	GKGCMC	1 cut(s)	917
BamHI	GGATCC	3 cut(s)	205, 694, 950
BanII	GRGCYC	1 cut(s)	2291
BauI	CACGAG	1 cut(s)	1407
BbsI	GAAGAC	1 cut(s)	1837
Bbv12I	GWGCWC	2 cut(s)	1356, 1461
BbvI	GCAGC	5 cut(s)	1377, 1732, 2060, 2068, 2239
BccI	CCATC	7 cut(s)	105, 1175, 1406, 1578, 1691, 1749, 1940
BciT130I	CCWGG	2 cut(s)	1644, 2206
BciVI	GTATCC	2 cut(s)	1083, 1418
BclI	TGATCA	1 cut(s)	142
BcoDI	GTCTC	5 cut(s)	297, 1099, 1642, 1877, 1929
BfaI	CTAG	7 cut(s)	278, 927, 1328, 1508, 1596, 1724, 1901
BfmI	CTRYAG	1 cut(s)	1931
BfoI	RGCGCY	1 cut(s)	2155
BfrI	CTTAAG	1 cut(s)	836
BfuAI	ACCTGC	1 cut(s)	1671
BfuI	GTATCC	2 cut(s)	1083, 1418
BisI	GCNGC	5 cut(s)	1391, 1721, 2049, 2082, 2228
BlsI	GCNGC	5 cut(s)	1392, 1722, 2050, 2083, 2229
BmcAI	AGTACT	1 cut(s)	690
Bme1390I	CCNGG	2 cut(s)	1644, 2206
BmeT110I	CYCGRG	2 cut(s)	57, 1116
BmgT120I	GGNCC	3 cut(s)	650, 2069, 2201
BmiI	GGNNCC	5 cut(s)	207, 696, 952, 1275, 2203
BmrFI	CCNGG	2 cut(s)	1644, 2206
BmrI	ACTGGG	2 cut(s)	483, 2026
BmsI	GCATC	8 cut(s)	24, 233, 320, 746, 840, 890, 1357, 2070
BmtI	GCTAGC	2 cut(s)	281, 1727
BmuI	ACTGGG	2 cut(s)	483, 2026
BoxI	GACNNNNGTC	1 cut(s)	74
BpiI	GAAGAC	1 cut(s)	1837
BplI	GAGNNNNNCTC	2 cut(s)	436, 468
Bpu10I	CCTNAGC	1 cut(s)	348
BpuEI	CTTGAG	2 cut(s)	166, 1364
BsaAI	YACGTR	2 cut(s)	530, 818
BsaBI	GATNNNNATC	1 cut(s)	1967
BsaI	GGTCTC	1 cut(s)	1642
BsaJI	CCNNGG	9 cut(s)	653, 1577, 1643, 1743, 1872, 2158, 2204, 2205, 2271
BsaWI	WCCGGW	1 cut(s)	1243
Bsc4I	CCNNNNNNNGG	4 cut(s)	960, 990, 1564, 2077
Bse1I	ACTGG	8 cut(s)	307, 396, 489, 696, 1053, 1198, 1433, 2021
Bse3DI	GCAATG	2 cut(s)	876, 1712
Bse8I	GATNNNNATC	1 cut(s)	1967
BseBI	CCWGG	2 cut(s)	1644, 2206
BseDI	CCNNGG	9 cut(s)	653, 1577, 1643, 1743, 1872, 2158, 2204, 2205, 2271
BseGI	GGATG	5 cut(s)	97, 248, 335, 831, 1760
BseJI	GATNNNNATC	1 cut(s)	1967
BseLI	CCNNNNNNNGG	4 cut(s)	960, 990, 1564, 2077
BseMI	GCAATG	2 cut(s)	876, 1712
BseMII	CTCAG	5 cut(s)	273, 801, 990, 1125, 2028
BseNI	ACTGG	8 cut(s)	307, 396, 489, 696, 1053, 1198, 1433, 2021
BseRI	GAGGAG	2 cut(s)	285, 2022
BseSI	GKGCMC	1 cut(s)	917
BseXI	GCAGC	5 cut(s)	1377, 1732, 2060, 2068, 2239
BshFI	GGCC	4 cut(s)	651, 858, 2070, 2202
BsiHKAI	GWGCWC	2 cut(s)	1356, 1461
BsiHKCI	CYCGRG	2 cut(s)	57, 1116
BsiSI	CCGG	1 cut(s)	1244
BslFI	GGGAC	2 cut(s)	353, 967
BslI	CCNNNNNNNGG	4 cut(s)	960, 990, 1564, 2077
BsmAI	GTCTC	5 cut(s)	297, 1099, 1642, 1877, 1929
BsmFI	GGGAC	2 cut(s)	353, 967
BsmI	GAATGC	1 cut(s)	2136
BsnI	GGCC	4 cut(s)	651, 858, 2070, 2202
Bso31I	GGTCTC	1 cut(s)	1642
BsoBI	CYCGRG	2 cut(s)	57, 1116
Bsp1286I	GDGCHC	4 cut(s)	917, 1356, 1461, 2291
Bsp1407I	TGTACA	1 cut(s)	27
Bsp143I	GATC	9 cut(s)	125, 142, 187, 205, 694, 871, 950, 1030, 1623
Bsp19I	CCATGG	2 cut(s)	653, 2158
BspACI	CCGC	5 cut(s)	485, 977, 1710, 1849, 1866
BspANI	GGCC	4 cut(s)	651, 858, 2070, 2202
BspCNI	CTCAG	5 cut(s)	274, 802, 991, 1124, 2029
BspHI	TCATGA	1 cut(s)	12
BspLI	GGNNCC	5 cut(s)	207, 696, 952, 1275, 2203
BspMI	ACCTGC	1 cut(s)	1671
BspOI	GCTAGC	2 cut(s)	281, 1727
BspPI	GGATC	9 cut(s)	182, 200, 213, 689, 702, 879, 945, 958, 1038
BspTI	CTTAAG	1 cut(s)	836
BspTNI	GGTCTC	1 cut(s)	1642
BsrBI	CCGCTC	1 cut(s)	1868
BsrDI	GCAATG	2 cut(s)	876, 1712
BsrGI	TGTACA	1 cut(s)	27
BsrI	ACTGG	8 cut(s)	307, 396, 489, 696, 1053, 1198, 1433, 2021
BssECI	CCNNGG	9 cut(s)	653, 1577, 1643, 1743, 1872, 2158, 2204, 2205, 2271
BssMI	GATC	9 cut(s)	125, 142, 187, 205, 694, 871, 950, 1030, 1623
BssSI	CACGAG	1 cut(s)	1407
BssT1I	CCWWGG	3 cut(s)	653, 1872, 2158
Bst2BI	CACGAG	1 cut(s)	1407
Bst2UI	CCWGG	2 cut(s)	1644, 2206
Bst4CI	ACNGT	5 cut(s)	94, 548, 805, 1440, 1763
Bst6I	CTCTTC	3 cut(s)	1134, 1414, 1953
BstAFI	CTTAAG	1 cut(s)	836
BstAUI	TGTACA	1 cut(s)	27
BstBAI	YACGTR	2 cut(s)	530, 818
BstC8I	GCNNGC	7 cut(s)	169, 275, 279, 996, 1524, 1725, 2232
BstDEI	CTNAG	7 cut(s)	282, 348, 810, 860, 999, 1111, 2037
BstDSI	CCRYGG	2 cut(s)	653, 2158
BstENI	CCTNNNNNAGG	1 cut(s)	958
BstF5I	GGATG	5 cut(s)	97, 248, 335, 831, 1760
BstH2I	RGCGCY	1 cut(s)	2155
BstHHI	GCGC	1 cut(s)	2154
BstKTI	GATC	9 cut(s)	128, 145, 190, 208, 697, 874, 953, 1033, 1626
BstMAI	GTCTC	5 cut(s)	297, 1099, 1642, 1877, 1929
BstMBI	GATC	9 cut(s)	125, 142, 187, 205, 694, 871, 950, 1030, 1623
BstMWI	GCNNNNNNNGC	5 cut(s)	855, 1677, 1729, 2067, 2236
BstNI	CCWGG	2 cut(s)	1644, 2206
BstNSI	RCATGY	2 cut(s)	2004, 2061
BstPAI	GACNNNNGTC	1 cut(s)	74
BstSCI	CCNGG	2 cut(s)	1642, 2204
BstSFI	CTRYAG	1 cut(s)	1931
BstSLI	GKGCMC	1 cut(s)	917
BstV1I	GCAGC	5 cut(s)	1377, 1732, 2060, 2068, 2239
BstV2I	GAAGAC	1 cut(s)	1837
BstX2I	RGATCY	6 cut(s)	187, 205, 694, 871, 950, 1030
BstYI	RGATCY	6 cut(s)	187, 205, 694, 871, 950, 1030
BsuI	GTATCC	2 cut(s)	1083, 1418
BsuRI	GGCC	4 cut(s)	651, 858, 2070, 2202
BtgI	CCRYGG	2 cut(s)	653, 2158
BtgZI	GCGATG	1 cut(s)	1597
BtsCI	GGATG	5 cut(s)	97, 248, 335, 831, 1760
BtsI	GCAGTG	1 cut(s)	714
BtsIMutI	CAGTG	6 cut(s)	135, 403, 496, 714, 801, 1191
BveI	ACCTGC	1 cut(s)	1671
Cac8I	GCNNGC	7 cut(s)	169, 275, 279, 996, 1524, 1725, 2232
CaiI	CAGNNNCTG	1 cut(s)	1610
CciI	TCATGA	1 cut(s)	12
CfoI	GCGC	1 cut(s)	2154
Cfr13I	GGNCC	3 cut(s)	650, 2069, 2201
CseI	GACGC	1 cut(s)	26
Csp6I	GTAC	4 cut(s)	28, 689, 1360, 1835
CviQI	GTAC	4 cut(s)	28, 689, 1360, 1835
DdeI	CTNAG	7 cut(s)	282, 348, 810, 860, 999, 1111, 2037
DpnI	GATC	9 cut(s)	127, 144, 189, 207, 696, 873, 952, 1032, 1625
DpnII	GATC	9 cut(s)	125, 142, 187, 205, 694, 871, 950, 1030, 1623
DraI	TTTAAA	1 cut(s)	1656
Eam1104I	CTCTTC	3 cut(s)	1134, 1414, 1953
EarI	CTCTTC	3 cut(s)	1134, 1414, 1953
EciI	GGCGGA	1 cut(s)	474
Eco130I	CCWWGG	3 cut(s)	653, 1872, 2158
Eco24I	GRGCYC	1 cut(s)	2291
Eco31I	GGTCTC	1 cut(s)	1642
Eco32I	GATATC	1 cut(s)	966
Eco57I	CTGAAG	1 cut(s)	1977
Eco88I	CYCGRG	2 cut(s)	57, 1116
EcoNI	CCTNNNNNAGG	1 cut(s)	958
EcoO109I	RGGNCCY	1 cut(s)	2201
EcoRII	CCWGG	2 cut(s)	1642, 2204
EcoRV	GATATC	1 cut(s)	966
EcoT14I	CCWWGG	3 cut(s)	653, 1872, 2158
EcoT22I	ATGCAT	2 cut(s)	739, 2063
EcoT38I	GRGCYC	1 cut(s)	2291
ErhI	CCWWGG	3 cut(s)	653, 1872, 2158
FalI	AAGNNNNNCTT	2 cut(s)	2169, 2201
FaqI	GGGAC	2 cut(s)	353, 967
FbaI	TGATCA	1 cut(s)	142
FblI	GTMKAC	2 cut(s)	1308, 2092
Fnu4HI	GCNGC	5 cut(s)	1391, 1721, 2049, 2082, 2228
FokI	GGATG	5 cut(s)	84, 255, 342, 818, 1767
FriOI	GRGCYC	1 cut(s)	2291
Fsp4HI	GCNGC	5 cut(s)	1391, 1721, 2049, 2082, 2228
FspBI	CTAG	7 cut(s)	278, 927, 1328, 1508, 1596, 1724, 1901
GlaI	GCGC	1 cut(s)	2153
GluI	GCNGC	5 cut(s)	1391, 1721, 2049, 2082, 2228
HaeII	RGCGCY	1 cut(s)	2155
HaeIII	GGCC	4 cut(s)	651, 858, 2070, 2202
HapII	CCGG	1 cut(s)	1244
HgaI	GACGC	1 cut(s)	26
HhaI	GCGC	1 cut(s)	2154
Hin6I	GCGC	1 cut(s)	2152
HinP1I	GCGC	1 cut(s)	2152
HincII	GTYRAC	5 cut(s)	69, 90, 1791, 2014, 2093
HindII	GTYRAC	5 cut(s)	69, 90, 1791, 2014, 2093
HindIII	AAGCTT	1 cut(s)	2183
HpaI	GTTAAC	1 cut(s)	2014
HpaII	CCGG	1 cut(s)	1244
HphI	GGTGA	5 cut(s)	152, 545, 1034, 1919, 2186
Hpy166II	GTNNAC	7 cut(s)	69, 90, 1025, 1309, 1791, 2014, 2093
Hpy8I	GTNNAC	7 cut(s)	69, 90, 1025, 1309, 1791, 2014, 2093
Hpy99I	CGWCG	1 cut(s)	42
HpyAV	CCTTC	2 cut(s)	1568, 1742
HpyCH4III	ACNGT	5 cut(s)	94, 548, 805, 1440, 1763
HpyCH4IV	ACGT	3 cut(s)	529, 817, 853
HpyF10VI	GCNNNNNNNGC	5 cut(s)	855, 1677, 1729, 2067, 2236
HpyF3I	CTNAG	7 cut(s)	282, 348, 810, 860, 999, 1111, 2037
HpySE526I	ACGT	3 cut(s)	529, 817, 853
HspAI	GCGC	1 cut(s)	2152
Ksp22I	TGATCA	1 cut(s)	142
KspAI	GTTAAC	1 cut(s)	2014
Kzo9I	GATC	9 cut(s)	125, 142, 187, 205, 694, 871, 950, 1030, 1623
LmnI	GCTCC	4 cut(s)	1279, 1456, 1702, 1873
Lsp1109I	GCAGC	5 cut(s)	1377, 1732, 2060, 2068, 2239
LweI	GCATC	8 cut(s)	24, 233, 320, 746, 840, 890, 1357, 2070
MaeI	CTAG	7 cut(s)	278, 927, 1328, 1508, 1596, 1724, 1901
MaeII	ACGT	3 cut(s)	529, 817, 853
MaeIII	GTNAC	5 cut(s)	334, 386, 1503, 1983, 2053
MalI	GATC	9 cut(s)	127, 144, 189, 207, 696, 873, 952, 1032, 1625
MbiI	CCGCTC	1 cut(s)	1868
MboI	GATC	9 cut(s)	125, 142, 187, 205, 694, 871, 950, 1030, 1623
MfeI	CAATTG	2 cut(s)	1446, 1665
MflI	RGATCY	6 cut(s)	187, 205, 694, 871, 950, 1030
MhlI	GDGCHC	4 cut(s)	917, 1356, 1461, 2291
MlyI	GAGTC	6 cut(s)	30, 989, 1563, 1616, 1931, 2208
MmeI	TCCRAC	1 cut(s)	1100
Mph1103I	ATGCAT	2 cut(s)	739, 2063
MroXI	GAANNNNTTC	3 cut(s)	547, 1400, 1962
MspA1I	CMGCKG	1 cut(s)	2051
MspCI	CTTAAG	1 cut(s)	836
MspI	CCGG	1 cut(s)	1244
MspR9I	CCNGG	2 cut(s)	1644, 2206
MunI	CAATTG	2 cut(s)	1446, 1665
Mva1269I	GAATGC	1 cut(s)	2136
MvaI	CCWGG	2 cut(s)	1644, 2206
MwoI	GCNNNNNNNGC	5 cut(s)	855, 1677, 1729, 2067, 2236
NcoI	CCATGG	2 cut(s)	653, 2158
NdeII	GATC	9 cut(s)	125, 142, 187, 205, 694, 871, 950, 1030, 1623
NheI	GCTAGC	2 cut(s)	277, 1723
NlaIV	GGNNCC	5 cut(s)	207, 696, 952, 1275, 2203
NmeAIII	GCCGAG	3 cut(s)	1016, 1768, 2296
NmuCI	GTSAC	1 cut(s)	386
NsiI	ATGCAT	2 cut(s)	739, 2063
NspI	RCATGY	2 cut(s)	2004, 2061
PaeR7I	CTCGAG	2 cut(s)	57, 1116
PagI	TCATGA	1 cut(s)	12
PasI	CCCWGGG	1 cut(s)	2205
PctI	GAATGC	1 cut(s)	2136
PdmI	GAANNNNTTC	3 cut(s)	547, 1400, 1962
PfeI	GAWTC	5 cut(s)	236, 467, 1226, 1265, 1963
PkrI	GCNGC	5 cut(s)	1392, 1722, 2050, 2083, 2229
PleI	GAGTC	6 cut(s)	29, 988, 1562, 1615, 1930, 2208
PpsI	GAGTC	6 cut(s)	29, 988, 1562, 1615, 1930, 2208
Ppu21I	YACGTR	2 cut(s)	530, 818
PshAI	GACNNNNGTC	1 cut(s)	74
Psp6I	CCWGG	2 cut(s)	1642, 2204
PspGI	CCWGG	2 cut(s)	1642, 2204
PspN4I	GGNNCC	5 cut(s)	207, 696, 952, 1275, 2203
PspPI	GGNCC	3 cut(s)	650, 2069, 2201
PspXI	VCTCGAGB	1 cut(s)	57
PstNI	CAGNNNCTG	1 cut(s)	1610
PsuI	RGATCY	6 cut(s)	187, 205, 694, 871, 950, 1030
PvuII	CAGCTG	1 cut(s)	2051
RsaI	GTAC	4 cut(s)	29, 690, 1361, 1836
RsaNI	GTAC	4 cut(s)	28, 689, 1360, 1835
SalI	GTCGAC	1 cut(s)	2091
SatI	GCNGC	5 cut(s)	1391, 1721, 2049, 2082, 2228
Sau3AI	GATC	9 cut(s)	125, 142, 187, 205, 694, 871, 950, 1030, 1623
Sau96I	GGNCC	3 cut(s)	650, 2069, 2201
ScaI	AGTACT	1 cut(s)	690
SchI	GAGTC	6 cut(s)	30, 989, 1563, 1616, 1931, 2208
ScrFI	CCNGG	2 cut(s)	1644, 2206
SduI	GDGCHC	4 cut(s)	917, 1356, 1461, 2291
SfaNI	GCATC	8 cut(s)	24, 233, 320, 746, 840, 890, 1357, 2070
SfcI	CTRYAG	1 cut(s)	1931
Sfr274I	CTCGAG	2 cut(s)	57, 1116
SlaI	CTCGAG	2 cut(s)	57, 1116
SmlI	CTYRAG	5 cut(s)	57, 181, 836, 1116, 1343
SmoI	CTYRAG	5 cut(s)	57, 181, 836, 1116, 1343
SsiI	CCGC	5 cut(s)	485, 977, 1710, 1849, 1866
SspI	AATATT	1 cut(s)	1736
SspMI	CTAG	7 cut(s)	278, 927, 1328, 1508, 1596, 1724, 1901
StyD4I	CCNGG	2 cut(s)	1642, 2204
StyI	CCWWGG	3 cut(s)	653, 1872, 2158
TaaI	ACNGT	5 cut(s)	94, 548, 805, 1440, 1763
TaiI	ACGT	3 cut(s)	532, 820, 856
TaqI	TCGA	7 cut(s)	58, 599, 631, 1117, 1920, 2005, 2092
TatI	WGTACW	2 cut(s)	27, 688
TfiI	GAWTC	5 cut(s)	236, 467, 1226, 1265, 1963
TscAI	CASTG	6 cut(s)	135, 403, 496, 714, 808, 1198
TseFI	GTSAC	1 cut(s)	386
TseI	GCWGC	5 cut(s)	1390, 1720, 2048, 2081, 2227
Tsp45I	GTSAC	1 cut(s)	386
TspGWI	ACGGA	1 cut(s)	658
TspRI	CASTG	6 cut(s)	135, 403, 496, 714, 808, 1198
Vha464I	CTTAAG	1 cut(s)	836
XagI	CCTNNNNNAGG	1 cut(s)	958
XapI	RAATTY	5 cut(s)	319, 577, 1104, 1332, 1938
XceI	RCATGY	2 cut(s)	2004, 2061
XcmI	CCANNNNNNNNNTGG	1 cut(s)	1581
XhoI	CTCGAG	2 cut(s)	57, 1116
XmiI	GTMKAC	2 cut(s)	1308, 2092
XmnI	GAANNNNTTC	3 cut(s)	547, 1400, 1962
XspI	CTAG	7 cut(s)	278, 927, 1328, 1508, 1596, 1724, 1901
ZrmI	AGTACT	1 cut(s)	690
Zsp2I	ATGCAT	2 cut(s)	739, 2063

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.