MD10G1128300.v1.1

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr10
OG0005359
Physical Location & Seq
Forward (+)
20902181 .. 20902708
528 bp
Loading structure...
UTR
Exon/CDS
Intron
MD10G1128300.v1.1.491

Sequence Viewer

Length: 528 bp
ATGGAATCCATTTCCAAGGAGATCTCCGCCGTTAAAAACCCTAAGCCCAATTCGCATTCCCATTCCCATTCCCACTCCCATTCCCCGACGTTTCTCCTCCACAACGGCTTCAGCGACGAGGTCGATGTCTCTGACATCGAGATGATCACGATCCAGACCGTCACCTACACCAGCCTCAGGGACCTCCTCCCGGCGTCGCCGCAGCCATCGATCATGTCACCGATTCACAATTCCAGCTGGCACGACGAGATTCCGATCAAGAACCCGCTCGTCAAGCATGCCGCGCTGGCGTACCTCCAGCCGATGTCCTCGCCGCCAGAGGTCGGCGATAAGGGATTATTCCGGATGATCCGGGAGAAGTGCAACTGTGAGTTCGGGTGCCTCGGTTGGTTCGGCGACGTCGTTTTGAAGACAGTTAGGGACGTGTTTGGTGGCGTTTGTGGTTGTACGAATAGTTTTGGCGAGATTGGGGATGAGGAAGACGAGGAGGGGGAGGAGGAGGAGGAGGAGTGTTTGAAGGTTGACTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

176

Amino Acids

19.38

Weight (kDa)

4.75

Isoelectric Point (pI)

62.87

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 402
AccB1I GGYRCC 1 cut(s) 378
AccBSI CCGCTC 1 cut(s) 268
AccII CGCG 1 cut(s) 284
AccIII TCCGGA 1 cut(s) 342
AciI CCGC 5 cut(s) 27, 200, 266, 282, 314
AclWI GGATC 2 cut(s) 145, 343
AcuI CTGAAG 1 cut(s) 94
AcyI GRCGYC 2 cut(s) 194, 399
AfaI GTAC 2 cut(s) 293, 448
AfiI CCNNNNNNNGG 3 cut(s) 177, 190, 323
AflIII ACRYGT 1 cut(s) 423
AgsI TTSAA 2 cut(s) 409, 517
AjiI CACGTC 1 cut(s) 424
AluBI AGCT 1 cut(s) 237
AluI AGCT 1 cut(s) 237
Alw26I GTCTC 1 cut(s) 133
AlwI GGATC 2 cut(s) 145, 343
Aor13HI TCCGGA 1 cut(s) 342
ApeKI GCWGC 1 cut(s) 202
AspLEI GCGC 1 cut(s) 286
AspS9I GGNCC 1 cut(s) 181
AsuC2I CCSGG 2 cut(s) 191, 353
AsuHPI GGTGA 2 cut(s) 154, 210
AvaII GGWCC 1 cut(s) 181
AxyI CCTNAGG 1 cut(s) 176
BanI GGYRCC 1 cut(s) 378
BbsI GAAGAC 2 cut(s) 416, 486
BbvI GCAGC 1 cut(s) 214
BccI CCATC 1 cut(s) 214
BceAI ACGGC 2 cut(s) 14, 121
BclI TGATCA 1 cut(s) 144
BcnI CCSGG 2 cut(s) 191, 353
BcoDI GTCTC 1 cut(s) 133
BglI GCCNNNNNGGC 1 cut(s) 287
BglII AGATCT 1 cut(s) 21
BisI GCNGC 4 cut(s) 200, 203, 282, 314
BlsI GCNGC 4 cut(s) 201, 204, 283, 315
Bme1390I CCNGG 2 cut(s) 191, 353
Bme18I GGWCC 1 cut(s) 181
BmgBI CACGTC 1 cut(s) 424
BmgT120I GGNCC 1 cut(s) 181
BmiI GGNNCC 2 cut(s) 182, 380
BmrFI CCNGG 2 cut(s) 191, 353
BpiI GAAGAC 2 cut(s) 416, 486
BpmI CTGGAG 1 cut(s) 281
Bpu10I CCTNAGC 1 cut(s) 42
BpuMI CCSGG 2 cut(s) 191, 353
Bsa29I ATCGAT 1 cut(s) 209
BsaBI GATNNNNATC 2 cut(s) 149, 254
BsaHI GRCGYC 2 cut(s) 194, 399
BsaJI CCNNGG 2 cut(s) 15, 382
BsaWI WCCGGW 1 cut(s) 342
BsaXI ACNNNNNCTCC 2 cut(s) 494, 524
Bsc4I CCNNNNNNNGG 3 cut(s) 177, 190, 323
Bse21I CCTNAGG 1 cut(s) 176
Bse8I GATNNNNATC 2 cut(s) 149, 254
BseAI TCCGGA 1 cut(s) 342
BseCI ATCGAT 1 cut(s) 209
BseDI CCNNGG 2 cut(s) 15, 382
BseGI GGATG 2 cut(s) 351, 478
BseJI GATNNNNATC 2 cut(s) 149, 254
BseLI CCNNNNNNNGG 3 cut(s) 177, 190, 323
BseMII CTCAG 1 cut(s) 190
BseRI GAGGAG 8 cut(s) 86, 176, 500, 509, 512, 515, 518, 521
BseXI GCAGC 1 cut(s) 214
Bsh1236I CGCG 1 cut(s) 284
BshNI GGYRCC 1 cut(s) 378
BshVI ATCGAT 1 cut(s) 209
BsiSI CCGG 3 cut(s) 191, 343, 352
BslFI GGGAC 2 cut(s) 194, 434
BslI CCNNNNNNNGG 3 cut(s) 177, 190, 323
BsmAI GTCTC 1 cut(s) 133
BsmFI GGGAC 2 cut(s) 194, 434
BsmI GAATGC 1 cut(s) 55
Bsp13I TCCGGA 1 cut(s) 342
Bsp143I GATC 6 cut(s) 21, 144, 150, 210, 255, 348
BspACI CCGC 5 cut(s) 27, 200, 266, 282, 314
BspCNI CTCAG 1 cut(s) 189
BspDI ATCGAT 1 cut(s) 209
BspEI TCCGGA 1 cut(s) 342
BspFNI CGCG 1 cut(s) 284
BspLI GGNNCC 2 cut(s) 182, 380
BspPI GGATC 2 cut(s) 145, 343
BspT107I GGYRCC 1 cut(s) 378
BsrBI CCGCTC 1 cut(s) 268
BssECI CCNNGG 2 cut(s) 15, 382
BssMI GATC 6 cut(s) 21, 144, 150, 210, 255, 348
BssNI GRCGYC 2 cut(s) 194, 399
BssT1I CCWWGG 1 cut(s) 15
Bst4CI ACNGT 3 cut(s) 160, 368, 415
BstACI GRCGYC 2 cut(s) 194, 399
BstC8I GCNNGC 3 cut(s) 239, 279, 288
BstDEI CTNAG 2 cut(s) 42, 176
BstF5I GGATG 2 cut(s) 351, 478
BstFNI CGCG 1 cut(s) 284
BstHHI GCGC 1 cut(s) 286
BstKTI GATC 6 cut(s) 24, 147, 153, 213, 258, 351
BstMAI GTCTC 1 cut(s) 133
BstMBI GATC 6 cut(s) 21, 144, 150, 210, 255, 348
BstMWI GCNNNNNNNGC 4 cut(s) 52, 274, 283, 287
BstNSI RCATGY 1 cut(s) 281
BstSCI CCNGG 2 cut(s) 189, 351
BstUI CGCG 1 cut(s) 284
BstV1I GCAGC 1 cut(s) 214
BstV2I GAAGAC 2 cut(s) 416, 486
BstX2I RGATCY 1 cut(s) 21
BstYI RGATCY 1 cut(s) 21
Bsu15I ATCGAT 1 cut(s) 209
Bsu36I CCTNAGG 1 cut(s) 176
BsuTUI ATCGAT 1 cut(s) 209
BtrI CACGTC 1 cut(s) 424
BtsCI GGATG 2 cut(s) 351, 478
Cac8I GCNNGC 3 cut(s) 239, 279, 288
CfoI GCGC 1 cut(s) 286
Cfr13I GGNCC 1 cut(s) 181
ClaI ATCGAT 1 cut(s) 209
CseI GACGC 1 cut(s) 183
Csp6I GTAC 2 cut(s) 292, 447
CviAII CATG 2 cut(s) 214, 278
CviJI RGCY 6 cut(s) 46, 108, 174, 205, 237, 301
CviKI_1 RGCY 6 cut(s) 46, 108, 174, 205, 237, 301
CviQI GTAC 2 cut(s) 292, 447
DdeI CTNAG 2 cut(s) 42, 176
DpnI GATC 6 cut(s) 23, 146, 152, 212, 257, 350
DpnII GATC 6 cut(s) 21, 144, 150, 210, 255, 348
EciI GGCGGA 1 cut(s) 16
Eco130I CCWWGG 1 cut(s) 15
Eco47I GGWCC 1 cut(s) 181
Eco57I CTGAAG 1 cut(s) 94
Eco81I CCTNAGG 1 cut(s) 176
EcoO109I RGGNCCY 1 cut(s) 181
EcoT14I CCWWGG 1 cut(s) 15
ErhI CCWWGG 1 cut(s) 15
FaeI CATG 2 cut(s) 217, 281
FaiI YATR 2 cut(s) 215, 279
FaqI GGGAC 2 cut(s) 194, 434
FatI CATG 2 cut(s) 213, 277
FauI CCCGC 1 cut(s) 273
FbaI TGATCA 1 cut(s) 144
Fnu4HI GCNGC 4 cut(s) 200, 203, 282, 314
FokI GGATG 2 cut(s) 358, 485
Fsp4HI GCNGC 4 cut(s) 200, 203, 282, 314
GlaI GCGC 1 cut(s) 285
GluI GCNGC 4 cut(s) 200, 203, 282, 314
GsuI CTGGAG 1 cut(s) 281
HapII CCGG 3 cut(s) 191, 343, 352
HgaI GACGC 1 cut(s) 183
HhaI GCGC 1 cut(s) 286
Hin1I GRCGYC 2 cut(s) 194, 399
Hin1II CATG 2 cut(s) 217, 281
Hin6I GCGC 1 cut(s) 284
HinP1I GCGC 1 cut(s) 284
HincII GTYRAC 1 cut(s) 523
HindII GTYRAC 1 cut(s) 523
HinfI GANTC 3 cut(s) 5, 223, 250
HpaII CCGG 3 cut(s) 191, 343, 352
HphI GGTGA 2 cut(s) 154, 210
Hpy166II GTNNAC 1 cut(s) 523
Hpy188I TCNGA 2 cut(s) 133, 255
Hpy188III TCNNGA 5 cut(s) 139, 148, 154, 259, 343
Hpy8I GTNNAC 1 cut(s) 523
Hpy99I CGWCG 6 cut(s) 91, 119, 199, 248, 401, 404
HpyAV CCTTC 1 cut(s) 511
HpyCH4III ACNGT 3 cut(s) 160, 368, 415
HpyCH4IV ACGT 3 cut(s) 89, 399, 423
HpyCH4V TGCA 1 cut(s) 363
HpyF10VI GCNNNNNNNGC 4 cut(s) 52, 274, 283, 287
HpyF3I CTNAG 2 cut(s) 42, 176
HpySE526I ACGT 3 cut(s) 89, 399, 423
Hsp92I GRCGYC 2 cut(s) 194, 399
Hsp92II CATG 2 cut(s) 217, 281
HspAI GCGC 1 cut(s) 284
Kpn2I TCCGGA 1 cut(s) 342
Ksp22I TGATCA 1 cut(s) 144
Kzo9I GATC 6 cut(s) 21, 144, 150, 210, 255, 348
Lsp1109I GCAGC 1 cut(s) 214
MaeII ACGT 3 cut(s) 89, 399, 423
MaeIII GTNAC 2 cut(s) 160, 216
MalI GATC 6 cut(s) 23, 146, 152, 212, 257, 350
MbiI CCGCTC 1 cut(s) 268
MboI GATC 6 cut(s) 21, 144, 150, 210, 255, 348
MboII GAAGA 2 cut(s) 421, 491
MflI RGATCY 1 cut(s) 21
MluCI AATT 2 cut(s) 49, 229
MroI TCCGGA 1 cut(s) 342
MseI TTAA 1 cut(s) 33
MslI CAYNNNNRTG 1 cut(s) 140
MspA1I CMGCKG 1 cut(s) 237
MspI CCGG 3 cut(s) 191, 343, 352
MspR9I CCNGG 2 cut(s) 191, 353
Mva1269I GAATGC 1 cut(s) 55
MvnI CGCG 1 cut(s) 284
MwoI GCNNNNNNNGC 4 cut(s) 52, 274, 283, 287
NciI CCSGG 2 cut(s) 191, 353
NdeII GATC 6 cut(s) 21, 144, 150, 210, 255, 348
NlaIII CATG 2 cut(s) 217, 281
NlaIV GGNNCC 2 cut(s) 182, 380
NmuCI GTSAC 2 cut(s) 160, 216
NspI RCATGY 1 cut(s) 281
PaeI GCATGC 1 cut(s) 281
PcsI WCGNNNNNNNCGW 2 cut(s) 111, 399
PctI GAATGC 1 cut(s) 55
PfeI GAWTC 3 cut(s) 5, 223, 250
PflFI GACNNNGTC 1 cut(s) 119
PfoI TCCNGGA 1 cut(s) 351
PkrI GCNGC 4 cut(s) 201, 204, 283, 315
PpuMI RGGWCCY 1 cut(s) 181
Psp5II RGGWCCY 1 cut(s) 181
PspN4I GGNNCC 2 cut(s) 182, 380
PspPI GGNCC 1 cut(s) 181
PspPPI RGGWCCY 1 cut(s) 181
PsuI RGATCY 1 cut(s) 21
PsyI GACNNNGTC 1 cut(s) 119
PvuII CAGCTG 1 cut(s) 237
RsaI GTAC 2 cut(s) 293, 448
RsaNI GTAC 2 cut(s) 292, 447
RseI CAYNNNNRTG 1 cut(s) 140
SaqAI TTAA 1 cut(s) 33
SatI GCNGC 4 cut(s) 200, 203, 282, 314
Sau3AI GATC 6 cut(s) 21, 144, 150, 210, 255, 348
Sau96I GGNCC 1 cut(s) 181
ScrFI CCNGG 2 cut(s) 191, 353
SinI GGWCC 1 cut(s) 181
SmiMI CAYNNNNRTG 1 cut(s) 140
SphI GCATGC 1 cut(s) 281
Sse9I AATT 2 cut(s) 49, 229
SsiI CCGC 5 cut(s) 27, 200, 266, 282, 314
StyD4I CCNGG 2 cut(s) 189, 351
StyI CCWWGG 1 cut(s) 15
TaaI ACNGT 3 cut(s) 160, 368, 415
TaiI ACGT 3 cut(s) 92, 402, 426
TaqI TCGA 3 cut(s) 123, 138, 209
TasI AATT 2 cut(s) 49, 229
TauI GCSGC 3 cut(s) 202, 284, 316
TfiI GAWTC 3 cut(s) 5, 223, 250
Tru1I TTAA 1 cut(s) 33
Tru9I TTAA 1 cut(s) 33
TseFI GTSAC 2 cut(s) 160, 216
TseI GCWGC 1 cut(s) 202
Tsp45I GTSAC 2 cut(s) 160, 216
Tth111I GACNNNGTC 1 cut(s) 119
VpaK11BI GGWCC 1 cut(s) 181
XceI RCATGY 1 cut(s) 281
ZraI GACGTC 1 cut(s) 400
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.