Prupe.8G169400_v2.0.a1

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
prunus_persica
Pp08
OG0005359
Physical Location & Seq
Forward (+)
17427256 .. 17428512
1257 bp
Loading structure...
UTR
Exon/CDS
Intron
Prupe.8G169400.1

Sequence Viewer

Length: 501 bp
ATGGAGATCTCCGCGATTAAAAACCCTAACCCTAACCCCAATTCCAATCACCATTCCCATTCTCCGAGCTTTCTCCTGCCCAACGGCTTCGGCGAAATCGAGATCTCCGATATCGAGATGATAACGATACAGACCGTGACCTACACCAGCCTCAAGGACCTGTTGCCGGCGTCGCCTCCGACGATCATGTCGCCGACGCAGAATTCCAGCTGGCACGAGATTCCGATAAAGAACCCGCTCGTAAAGCACGCGGCTTTGGCTTACCTGCAGCCGATGTCCACGCCGCCCGAGGTCGGGGACAAGGGGCTGTTGCGGATGCTCAGGGAGAAGTGCCTCTGCGAAGGCACGAACGGGGTCGGGTGCTTCTCGTGGCTCGGCGACGTCGTTTTGAGTGGAGTTAGGGATGTGTTTGGGGGCGTTTGTAGGAATGGGTGCGAGATTGGGGATGAGGAGGATGAGGATGATGAAGAGGACGACGACGAGTACGTGAAGGTTGACTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

167

Amino Acids

18.21

Weight (kDa)

4.43

Isoelectric Point (pI)

48.38

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 187
AatII GACGTC 1 cut(s) 384
Acc36I ACCTGC 1 cut(s) 273
AccBSI CCGCTC 1 cut(s) 238
AccII CGCG 2 cut(s) 14, 251
AciI CCGC 5 cut(s) 12, 236, 251, 284, 313
AcsI RAATTY 1 cut(s) 202
AcyI GRCGYC 2 cut(s) 170, 381
AfaI GTAC 1 cut(s) 485
AfiI CCNNNNNNNGG 3 cut(s) 166, 293, 294
AluBI AGCT 2 cut(s) 69, 210
AluI AGCT 2 cut(s) 69, 210
Ama87I CYCGRG 1 cut(s) 287
ApeKI GCWGC 1 cut(s) 268
ApoI RAATTY 1 cut(s) 202
AspS9I GGNCC 1 cut(s) 157
AsuHPI GGTGA 1 cut(s) 41
AvaI CYCGRG 1 cut(s) 287
AvaII GGWCC 1 cut(s) 157
BauI CACGAG 2 cut(s) 215, 367
BbvI GCAGC 1 cut(s) 280
BceAI ACGGC 1 cut(s) 100
BfmI CTRYAG 1 cut(s) 266
BfuAI ACCTGC 1 cut(s) 273
BglII AGATCT 2 cut(s) 6, 102
BisI GCNGC 3 cut(s) 252, 269, 284
BlsI GCNGC 3 cut(s) 253, 270, 285
Bme18I GGWCC 1 cut(s) 157
BmeT110I CYCGRG 1 cut(s) 287
BmgT120I GGNCC 1 cut(s) 157
BmsI GCATC 1 cut(s) 306
Bpu10I CCTNAGC 1 cut(s) 320
BpuEI CTTGAG 1 cut(s) 137
BsaAI YACGTR 1 cut(s) 487
BsaHI GRCGYC 2 cut(s) 170, 381
BsaJI CCNNGG 1 cut(s) 288
Bsc4I CCNNNNNNNGG 3 cut(s) 166, 293, 294
Bse118I RCCGGY 1 cut(s) 166
BseDI CCNNGG 1 cut(s) 288
BseGI GGATG 5 cut(s) 321, 409, 451, 460, 466
BseLI CCNNNNNNNGG 3 cut(s) 166, 293, 294
BseMII CTCAG 1 cut(s) 334
BseRI GAGGAG 1 cut(s) 464
BseXI GCAGC 1 cut(s) 280
Bsh1236I CGCG 2 cut(s) 14, 251
BsiHKCI CYCGRG 1 cut(s) 287
BsiSI CCGG 1 cut(s) 167
BslFI GGGAC 1 cut(s) 311
BslI CCNNNNNNNGG 3 cut(s) 166, 293, 294
BsmFI GGGAC 1 cut(s) 311
BsoBI CYCGRG 1 cut(s) 287
Bsp143I GATC 3 cut(s) 6, 102, 183
BspACI CCGC 5 cut(s) 12, 236, 251, 284, 313
BspCNI CTCAG 1 cut(s) 333
BspFNI CGCG 2 cut(s) 14, 251
BspMAI CTGCAG 1 cut(s) 270
BspMI ACCTGC 1 cut(s) 273
BsrBI CCGCTC 1 cut(s) 238
BsrFI RCCGGY 1 cut(s) 166
BssAI RCCGGY 1 cut(s) 166
BssECI CCNNGG 1 cut(s) 288
BssMI GATC 3 cut(s) 6, 102, 183
BssNI GRCGYC 2 cut(s) 170, 381
BssSI CACGAG 2 cut(s) 215, 367
Bst2BI CACGAG 2 cut(s) 215, 367
Bst4CI ACNGT 1 cut(s) 136
Bst6I CTCTTC 1 cut(s) 462
BstACI GRCGYC 2 cut(s) 170, 381
BstBAI YACGTR 1 cut(s) 487
BstC8I GCNNGC 3 cut(s) 168, 212, 249
BstDEI CTNAG 1 cut(s) 320
BstF5I GGATG 5 cut(s) 321, 409, 451, 460, 466
BstFNI CGCG 2 cut(s) 14, 251
BstKTI GATC 3 cut(s) 9, 105, 186
BstMBI GATC 3 cut(s) 6, 102, 183
BstMWI GCNNNNNNNGC 3 cut(s) 172, 244, 257
BstSFI CTRYAG 1 cut(s) 266
BstUI CGCG 2 cut(s) 14, 251
BstV1I GCAGC 1 cut(s) 280
BstX2I RGATCY 2 cut(s) 6, 102
BstYI RGATCY 2 cut(s) 6, 102
BtsCI GGATG 5 cut(s) 321, 409, 451, 460, 466
BveI ACCTGC 1 cut(s) 273
Cac8I GCNNGC 3 cut(s) 168, 212, 249
Cfr10I RCCGGY 1 cut(s) 166
Cfr13I GGNCC 1 cut(s) 157
CseI GACGC 2 cut(s) 159, 205
Csp6I GTAC 1 cut(s) 484
CviAII CATG 1 cut(s) 187
CviJI RGCY 9 cut(s) 69, 87, 150, 210, 254, 260, 271, 307, 373
CviKI_1 RGCY 9 cut(s) 69, 87, 150, 210, 254, 260, 271, 307, 373
CviQI GTAC 1 cut(s) 484
DdeI CTNAG 1 cut(s) 320
DpnI GATC 3 cut(s) 8, 104, 185
DpnII GATC 3 cut(s) 6, 102, 183
DrdI GACNNNNNNGTC 1 cut(s) 187
DseDI GACNNNNNNGTC 1 cut(s) 187
Eam1104I CTCTTC 1 cut(s) 462
EarI CTCTTC 1 cut(s) 462
Eco32I GATATC 1 cut(s) 112
Eco47I GGWCC 1 cut(s) 157
Eco88I CYCGRG 1 cut(s) 287
EcoO109I RGGNCCY 1 cut(s) 157
EcoRI GAATTC 1 cut(s) 202
EcoRV GATATC 1 cut(s) 112
FaeI CATG 1 cut(s) 190
FaiI YATR 1 cut(s) 188
FaqI GGGAC 1 cut(s) 311
FatI CATG 1 cut(s) 186
FauI CCCGC 1 cut(s) 243
Fnu4HI GCNGC 3 cut(s) 252, 269, 284
FokI GGATG 5 cut(s) 328, 416, 458, 467, 473
Fsp4HI GCNGC 3 cut(s) 252, 269, 284
GluI GCNGC 3 cut(s) 252, 269, 284
HapII CCGG 1 cut(s) 167
HgaI GACGC 2 cut(s) 159, 205
Hin1I GRCGYC 2 cut(s) 170, 381
Hin1II CATG 1 cut(s) 190
HincII GTYRAC 1 cut(s) 496
HindII GTYRAC 1 cut(s) 496
HinfI GANTC 1 cut(s) 220
HpaII CCGG 1 cut(s) 167
HphI GGTGA 1 cut(s) 41
Hpy166II GTNNAC 2 cut(s) 279, 496
Hpy188I TCNGA 4 cut(s) 66, 109, 180, 225
Hpy188III TCNNGA 2 cut(s) 100, 115
Hpy8I GTNNAC 2 cut(s) 279, 496
Hpy99I CGWCG 7 cut(s) 175, 184, 199, 383, 386, 479, 482
HpyAV CCTTC 2 cut(s) 335, 484
HpyCH4III ACNGT 1 cut(s) 136
HpyCH4IV ACGT 2 cut(s) 381, 486
HpyCH4V TGCA 1 cut(s) 268
HpyF10VI GCNNNNNNNGC 3 cut(s) 172, 244, 257
HpyF3I CTNAG 1 cut(s) 320
HpySE526I ACGT 2 cut(s) 381, 486
Hsp92I GRCGYC 2 cut(s) 170, 381
Hsp92II CATG 1 cut(s) 190
KroI GCCGGC 1 cut(s) 166
KroNI GCCGGC 1 cut(s) 168
Kzo9I GATC 3 cut(s) 6, 102, 183
LpnPI CCDG 8 cut(s) 89, 160, 173, 180, 196, 220, 278, 307
Lsp1109I GCAGC 1 cut(s) 280
LweI GCATC 1 cut(s) 306
MaeII ACGT 2 cut(s) 381, 486
MaeIII GTNAC 1 cut(s) 136
MalI GATC 3 cut(s) 8, 104, 185
MbiI CCGCTC 1 cut(s) 238
MboI GATC 3 cut(s) 6, 102, 183
MboII GAAGA 1 cut(s) 479
MflI RGATCY 2 cut(s) 6, 102
MluCI AATT 2 cut(s) 40, 202
MmeI TCCRAC 1 cut(s) 203
MnlI CCTC 8 cut(s) 161, 186, 283, 344, 442, 445, 451, 463
MroNI GCCGGC 1 cut(s) 166
MseI TTAA 1 cut(s) 18
MspA1I CMGCKG 1 cut(s) 210
MspI CCGG 1 cut(s) 167
MvnI CGCG 2 cut(s) 14, 251
MwoI GCNNNNNNNGC 3 cut(s) 172, 244, 257
NaeI GCCGGC 1 cut(s) 168
NdeII GATC 3 cut(s) 6, 102, 183
NgoMIV GCCGGC 1 cut(s) 166
NlaIII CATG 1 cut(s) 190
NmeAIII GCCGAG 1 cut(s) 354
NmuCI GTSAC 1 cut(s) 136
PcsI WCGNNNNNNNCGW 6 cut(s) 90, 96, 105, 179, 381, 483
PdiI GCCGGC 1 cut(s) 168
PfeI GAWTC 1 cut(s) 220
PkrI GCNGC 3 cut(s) 253, 270, 285
Ppu21I YACGTR 1 cut(s) 487
PpuMI RGGWCCY 1 cut(s) 157
Psp5II RGGWCCY 1 cut(s) 157
PspPI GGNCC 1 cut(s) 157
PspPPI RGGWCCY 1 cut(s) 157
PstI CTGCAG 1 cut(s) 270
PsuI RGATCY 2 cut(s) 6, 102
PvuII CAGCTG 1 cut(s) 210
RsaI GTAC 1 cut(s) 485
RsaNI GTAC 1 cut(s) 484
SaqAI TTAA 1 cut(s) 18
SatI GCNGC 3 cut(s) 252, 269, 284
Sau3AI GATC 3 cut(s) 6, 102, 183
Sau96I GGNCC 1 cut(s) 157
SetI ASST 9 cut(s) 71, 143, 162, 212, 267, 294, 384, 489, 495
SfaNI GCATC 1 cut(s) 306
SfcI CTRYAG 1 cut(s) 266
SinI GGWCC 1 cut(s) 157
SmlI CTYRAG 1 cut(s) 152
SmoI CTYRAG 1 cut(s) 152
Sse9I AATT 2 cut(s) 40, 202
SsiI CCGC 5 cut(s) 12, 236, 251, 284, 313
TaaI ACNGT 1 cut(s) 136
TaiI ACGT 2 cut(s) 384, 489
TaqI TCGA 2 cut(s) 99, 114
TasI AATT 2 cut(s) 40, 202
TauI GCSGC 2 cut(s) 254, 286
TfiI GAWTC 1 cut(s) 220
Tru1I TTAA 1 cut(s) 18
Tru9I TTAA 1 cut(s) 18
TseFI GTSAC 1 cut(s) 136
TseI GCWGC 1 cut(s) 268
Tsp45I GTSAC 1 cut(s) 136
TspDTI ATGAA 1 cut(s) 480
VpaK11BI GGWCC 1 cut(s) 157
XapI RAATTY 1 cut(s) 202
ZraI GACGTC 1 cut(s) 382
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.