pycom05g11960

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr5
OG0005359
Physical Location & Seq
Forward (+)
15464035 .. 15464670
636 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom05g11960.1

Sequence Viewer

Length: 636 bp
ATGGATCCAGCGCCCCCAAATGCGCGTACAGCGCGTGTGCCATCCCTTCTCCCGCTTCACAAAAACTCCAAACGCAACACAAAAACCCTCCCGAATCAGTCTCCCACTCCTTCTCTAATCGGAATCGGCGACGGAAAACATTCCATGGAGACCTCCGCCGTTAAAAACCCTACCCTCAATTCCCATTCCCATTCCCATTCCCCGACGTTTCTCCTCCACAACGGCTTCGGCGACGAGATCGAGATCTCCGACATCGAGATGGTCACGATCCAGACCGTCTCCTACACCAGCCTCAAGGACCTCCTCCCGGCATCGCCGCCGCTGTCAATCATGTCGCCTATCCACAATTCCAGCTGGCACGACGAGATTCCGATCAAGAACCCACTCGTCAAGCACGCCGCACTGGCGTATCTCCAGCCGATGTCCACGCCGCCGGAGGTCGGTGATAAAGGATTACTCCGGATGCTCGGGGAGAAGTGCCACTGTGAGTTGGGGTGCCTCGCGTGGGTTGGCGACGTCGTTTTGAAGACCGTTAGGGACGTGTTTGGTGGCGTTTGTGATCGTACGAATCGTTATGGCGAGATTGTAGATGAGGAGGGTGAGGATGACGACGAGGAGTTTGTGAAAGTTGACTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

212

Amino Acids

23.09

Weight (kDa)

5.41

Isoelectric Point (pI)

51.55

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 519
AccB1I GGYRCC 1 cut(s) 495
AccII CGCG 3 cut(s) 25, 34, 503
AccIII TCCGGA 1 cut(s) 459
AciI CCGC 6 cut(s) 53, 156, 317, 320, 399, 431
AclWI GGATC 2 cut(s) 12, 262
AcyI GRCGYC 1 cut(s) 516
AfaI GTAC 2 cut(s) 28, 565
AfiI CCNNNNNNNGG 3 cut(s) 307, 440, 505
AflIII ACRYGT 1 cut(s) 540
AgsI TTSAA 1 cut(s) 526
AjiI CACGTC 1 cut(s) 541
AluBI AGCT 1 cut(s) 354
AluI AGCT 1 cut(s) 354
Alw26I GTCTC 3 cut(s) 105, 143, 283
AlwI GGATC 2 cut(s) 12, 262
Ama87I CYCGRG 1 cut(s) 467
Aor13HI TCCGGA 1 cut(s) 459
ArsI GACNNNNNNTTYG 2 cut(s) 602, 634
Asp700I GAANNNNTTC 1 cut(s) 139
AspLEI GCGC 3 cut(s) 13, 25, 34
AspS9I GGNCC 1 cut(s) 298
AsuC2I CCSGG 1 cut(s) 308
AsuHPI GGTGA 2 cut(s) 455, 611
AvaI CYCGRG 1 cut(s) 467
AvaII GGWCC 1 cut(s) 298
BaeI ACNNNNGTAYC 2 cut(s) 392, 425
BamHI GGATCC 1 cut(s) 4
BanI GGYRCC 1 cut(s) 495
BbsI GAAGAC 1 cut(s) 533
BccI CCATC 2 cut(s) 49, 253
BceAI ACGGC 2 cut(s) 143, 238
BcnI CCSGG 1 cut(s) 308
BcoDI GTCTC 3 cut(s) 105, 143, 283
BfoI RGCGCY 1 cut(s) 14
BglI GCCNNNNNGGC 1 cut(s) 404
BglII AGATCT 1 cut(s) 243
BisI GCNGC 4 cut(s) 317, 320, 399, 431
BlsI GCNGC 4 cut(s) 318, 321, 400, 432
Bme1390I CCNGG 1 cut(s) 308
Bme18I GGWCC 1 cut(s) 298
BmeT110I CYCGRG 1 cut(s) 467
BmgBI CACGTC 1 cut(s) 541
BmgT120I GGNCC 1 cut(s) 298
BmiI GGNNCC 2 cut(s) 6, 497
BmrFI CCNGG 1 cut(s) 308
BmsI GCATC 2 cut(s) 320, 453
BpiI GAAGAC 1 cut(s) 533
BpmI CTGGAG 1 cut(s) 398
BpuEI CTTGAG 1 cut(s) 278
BpuMI CCSGG 1 cut(s) 308
BsaBI GATNNNNATC 2 cut(s) 242, 371
BsaHI GRCGYC 1 cut(s) 516
BsaI GGTCTC 1 cut(s) 143
BsaJI CCNNGG 1 cut(s) 144
BsaWI WCCGGW 1 cut(s) 459
BsaXI ACNNNNNCTCC 2 cut(s) 50, 80
Bsc4I CCNNNNNNNGG 3 cut(s) 307, 440, 505
Bse1I ACTGG 1 cut(s) 408
Bse8I GATNNNNATC 2 cut(s) 242, 371
BseAI TCCGGA 1 cut(s) 459
BseDI CCNNGG 1 cut(s) 144
BseGI GGATG 3 cut(s) 41, 468, 610
BseJI GATNNNNATC 2 cut(s) 242, 371
BseLI CCNNNNNNNGG 3 cut(s) 307, 440, 505
BseNI ACTGG 1 cut(s) 408
BseRI GAGGAG 4 cut(s) 203, 293, 608, 629
Bsh1236I CGCG 3 cut(s) 25, 34, 503
BshNI GGYRCC 1 cut(s) 495
BsiHKCI CYCGRG 1 cut(s) 467
BsiSI CCGG 3 cut(s) 308, 434, 460
BsiWI CGTACG 1 cut(s) 563
BslFI GGGAC 1 cut(s) 551
BslI CCNNNNNNNGG 3 cut(s) 307, 440, 505
BsmAI GTCTC 3 cut(s) 105, 143, 283
BsmBI CGTCTC 1 cut(s) 283
BsmFI GGGAC 1 cut(s) 551
Bso31I GGTCTC 1 cut(s) 143
BsoBI CYCGRG 1 cut(s) 467
Bsp13I TCCGGA 1 cut(s) 459
Bsp143I GATC 6 cut(s) 4, 237, 243, 267, 372, 559
Bsp19I CCATGG 1 cut(s) 144
BspACI CCGC 6 cut(s) 53, 156, 317, 320, 399, 431
BspEI TCCGGA 1 cut(s) 459
BspFNI CGCG 3 cut(s) 25, 34, 503
BspLI GGNNCC 2 cut(s) 6, 497
BspPI GGATC 2 cut(s) 12, 262
BspT107I GGYRCC 1 cut(s) 495
BspTNI GGTCTC 1 cut(s) 143
BsrI ACTGG 1 cut(s) 408
BssECI CCNNGG 1 cut(s) 144
BssMI GATC 6 cut(s) 4, 237, 243, 267, 372, 559
BssNI GRCGYC 1 cut(s) 516
BssT1I CCWWGG 1 cut(s) 144
Bst4CI ACNGT 3 cut(s) 277, 485, 532
BstACI GRCGYC 1 cut(s) 516
BstC8I GCNNGC 2 cut(s) 356, 396
BstDSI CCRYGG 1 cut(s) 144
BstF5I GGATG 3 cut(s) 41, 468, 610
BstFNI CGCG 3 cut(s) 25, 34, 503
BstH2I RGCGCY 1 cut(s) 14
BstHHI GCGC 3 cut(s) 13, 25, 34
BstKTI GATC 6 cut(s) 7, 240, 246, 270, 375, 562
BstMAI GTCTC 3 cut(s) 105, 143, 283
BstMBI GATC 6 cut(s) 4, 237, 243, 267, 372, 559
BstMWI GCNNNNNNNGC 3 cut(s) 29, 31, 404
BstSCI CCNGG 1 cut(s) 306
BstUI CGCG 3 cut(s) 25, 34, 503
BstV2I GAAGAC 1 cut(s) 533
BstX2I RGATCY 2 cut(s) 4, 243
BstYI RGATCY 2 cut(s) 4, 243
BtgI CCRYGG 1 cut(s) 144
BtgZI GCGATG 1 cut(s) 297
BtrI CACGTC 1 cut(s) 541
BtsCI GGATG 3 cut(s) 41, 468, 610
BtsIMutI CAGTG 2 cut(s) 401, 481
Cac8I GCNNGC 2 cut(s) 356, 396
CfoI GCGC 3 cut(s) 13, 25, 34
Cfr13I GGNCC 1 cut(s) 298
Csp6I GTAC 2 cut(s) 27, 564
CviAII CATG 2 cut(s) 145, 331
CviJI RGCY 4 cut(s) 225, 291, 354, 418
CviKI_1 RGCY 4 cut(s) 225, 291, 354, 418
CviQI GTAC 2 cut(s) 27, 564
DpnI GATC 6 cut(s) 6, 239, 245, 269, 374, 561
DpnII GATC 6 cut(s) 4, 237, 243, 267, 372, 559
EciI GGCGGA 1 cut(s) 145
Eco130I CCWWGG 1 cut(s) 144
Eco31I GGTCTC 1 cut(s) 143
Eco47I GGWCC 1 cut(s) 298
Eco88I CYCGRG 1 cut(s) 467
EcoO109I RGGNCCY 1 cut(s) 298
EcoT14I CCWWGG 1 cut(s) 144
ErhI CCWWGG 1 cut(s) 144
Esp3I CGTCTC 1 cut(s) 283
FaeI CATG 2 cut(s) 148, 334
FaiI YATR 3 cut(s) 146, 332, 576
FaqI GGGAC 1 cut(s) 551
FatI CATG 2 cut(s) 144, 330
FauI CCCGC 1 cut(s) 60
Fnu4HI GCNGC 4 cut(s) 317, 320, 399, 431
FokI GGATG 3 cut(s) 28, 475, 617
Fsp4HI GCNGC 4 cut(s) 317, 320, 399, 431
GlaI GCGC 3 cut(s) 12, 24, 33
GluI GCNGC 4 cut(s) 317, 320, 399, 431
GsuI CTGGAG 1 cut(s) 398
HaeII RGCGCY 1 cut(s) 14
HapII CCGG 3 cut(s) 308, 434, 460
HhaI GCGC 3 cut(s) 13, 25, 34
Hin1I GRCGYC 1 cut(s) 516
Hin1II CATG 2 cut(s) 148, 334
Hin6I GCGC 3 cut(s) 11, 23, 32
HinP1I GCGC 3 cut(s) 11, 23, 32
HincII GTYRAC 1 cut(s) 631
HindII GTYRAC 1 cut(s) 631
HinfI GANTC 4 cut(s) 94, 123, 367, 568
HpaII CCGG 3 cut(s) 308, 434, 460
HphI GGTGA 2 cut(s) 455, 611
Hpy166II GTNNAC 2 cut(s) 426, 631
Hpy188I TCNGA 3 cut(s) 122, 250, 372
Hpy188III TCNNGA 7 cut(s) 91, 241, 256, 265, 271, 376, 460
Hpy8I GTNNAC 2 cut(s) 426, 631
Hpy99I CGWCG 7 cut(s) 134, 208, 236, 365, 518, 521, 614
HpyAV CCTTC 2 cut(s) 56, 120
HpyCH4III ACNGT 3 cut(s) 277, 485, 532
HpyCH4IV ACGT 3 cut(s) 206, 516, 540
HpyF10VI GCNNNNNNNGC 3 cut(s) 29, 31, 404
HpySE526I ACGT 3 cut(s) 206, 516, 540
Hsp92I GRCGYC 1 cut(s) 516
Hsp92II CATG 2 cut(s) 148, 334
HspAI GCGC 3 cut(s) 11, 23, 32
Kpn2I TCCGGA 1 cut(s) 459
Kzo9I GATC 6 cut(s) 4, 237, 243, 267, 372, 559
LweI GCATC 2 cut(s) 320, 453
MaeII ACGT 3 cut(s) 206, 516, 540
MaeIII GTNAC 1 cut(s) 262
MalI GATC 6 cut(s) 6, 239, 245, 269, 374, 561
MboI GATC 6 cut(s) 4, 237, 243, 267, 372, 559
MboII GAAGA 1 cut(s) 538
MflI RGATCY 2 cut(s) 4, 243
MluCI AATT 2 cut(s) 178, 346
MmeI TCCRAC 1 cut(s) 273
MroI TCCGGA 1 cut(s) 459
MroXI GAANNNNTTC 1 cut(s) 139
MseI TTAA 1 cut(s) 162
MslI CAYNNNNRTG 1 cut(s) 257
MspA1I CMGCKG 2 cut(s) 322, 354
MspI CCGG 3 cut(s) 308, 434, 460
MspR9I CCNGG 1 cut(s) 308
MvnI CGCG 3 cut(s) 25, 34, 503
MwoI GCNNNNNNNGC 3 cut(s) 29, 31, 404
NciI CCSGG 1 cut(s) 308
NcoI CCATGG 1 cut(s) 144
NdeII GATC 6 cut(s) 4, 237, 243, 267, 372, 559
NlaIII CATG 2 cut(s) 148, 334
NlaIV GGNNCC 2 cut(s) 6, 497
NmuCI GTSAC 1 cut(s) 262
PcsI WCGNNNNNNNCGW 4 cut(s) 126, 228, 246, 568
PdmI GAANNNNTTC 1 cut(s) 139
PfeI GAWTC 4 cut(s) 94, 123, 367, 568
Pfl23II CGTACG 1 cut(s) 563
PkrI GCNGC 4 cut(s) 318, 321, 400, 432
PpuMI RGGWCCY 1 cut(s) 298
Psp5II RGGWCCY 1 cut(s) 298
PspLI CGTACG 1 cut(s) 563
PspN4I GGNNCC 2 cut(s) 6, 497
PspPI GGNCC 1 cut(s) 298
PspPPI RGGWCCY 1 cut(s) 298
PsuI RGATCY 2 cut(s) 4, 243
PvuII CAGCTG 1 cut(s) 354
RsaI GTAC 2 cut(s) 28, 565
RsaNI GTAC 2 cut(s) 27, 564
RseI CAYNNNNRTG 1 cut(s) 257
SaqAI TTAA 1 cut(s) 162
SatI GCNGC 4 cut(s) 317, 320, 399, 431
Sau3AI GATC 6 cut(s) 4, 237, 243, 267, 372, 559
Sau96I GGNCC 1 cut(s) 298
ScrFI CCNGG 1 cut(s) 308
SetI ASST 7 cut(s) 155, 209, 303, 356, 441, 519, 543
SfaNI GCATC 2 cut(s) 320, 453
SinI GGWCC 1 cut(s) 298
SmiMI CAYNNNNRTG 1 cut(s) 257
SmlI CTYRAG 1 cut(s) 293
SmoI CTYRAG 1 cut(s) 293
Sse9I AATT 2 cut(s) 178, 346
SsiI CCGC 6 cut(s) 53, 156, 317, 320, 399, 431
StyD4I CCNGG 1 cut(s) 306
StyI CCWWGG 1 cut(s) 144
TaaI ACNGT 3 cut(s) 277, 485, 532
TaiI ACGT 3 cut(s) 209, 519, 543
TaqI TCGA 2 cut(s) 240, 255
TasI AATT 2 cut(s) 178, 346
TauI GCSGC 4 cut(s) 319, 322, 401, 433
TfiI GAWTC 4 cut(s) 94, 123, 367, 568
Tru1I TTAA 1 cut(s) 162
Tru9I TTAA 1 cut(s) 162
TscAI CASTG 2 cut(s) 408, 488
TseFI GTSAC 1 cut(s) 262
Tsp45I GTSAC 1 cut(s) 262
TspGWI ACGGA 1 cut(s) 147
TspRI CASTG 2 cut(s) 408, 488
VpaK11BI GGWCC 1 cut(s) 298
XmnI GAANNNNTTC 1 cut(s) 139
ZraI GACGTC 1 cut(s) 517
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.