Rmu_sc0003852.1_g000009

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_multiflora
Rmu_sc0003852.1
OG0005359
Physical Location & Seq
Reverse (-)
22157 .. 22606
450 bp
Loading structure...
UTR
Exon/CDS
Intron
Rmu_sc0003852.1_g000009.1.cds

Sequence Viewer

Length: 450 bp
atgacctcgagtttatccactcgatccacctccgtcgtcgtccccactaagatcagccacctctcctcctcctcatcctccaccactcccaccaataagcctcaccagaactgcagtttcctaaccgaaaaaggcgctgtgacgtcacgccatcggccgccggagctcgacctggagcttctgtccctcaaaagctcgagtcctacacctcactcaaggacctcatcctcctcttccttcgtcacggtccagtccccgatgcccaacttaaccaccaactccggctacgagatatccatccgtagctgcctcgtcaagcgagtggcctgggcctatctccaatccgtgtccttcacttgcggcccacacttgctccgccgcctctggctcaagttcaccccctgcctctccttcatctcccactgccatcattttggaagtttcatttag
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

149

Amino Acids

16.38

Weight (kDa)

9.89

Isoelectric Point (pI)

67.19

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 146
AciI CCGC 4 cut(s) 158, 360, 376, 379
AclWI GGATC 1 cut(s) 18
AcoI YGGCCR 1 cut(s) 155
AcyI GRCGYC 1 cut(s) 143
AjnI CCWGG 2 cut(s) 171, 326
AluBI AGCT 4 cut(s) 166, 178, 195, 306
AluI AGCT 4 cut(s) 166, 178, 195, 306
Alw21I GWGCWC 1 cut(s) 168
AlwI GGATC 1 cut(s) 18
Ama87I CYCGRG 2 cut(s) 7, 196
AoxI GGCC 4 cut(s) 155, 324, 330, 361
ApeKI GCWGC 1 cut(s) 306
AspLEI GCGC 1 cut(s) 137
AspS9I GGNCC 4 cut(s) 219, 247, 330, 362
AsuHPI GGTGA 2 cut(s) 95, 388
AvaI CYCGRG 2 cut(s) 7, 196
AvaII GGWCC 2 cut(s) 219, 247
BanII GRGCYC 1 cut(s) 168
Bbv12I GWGCWC 1 cut(s) 168
BbvI GCAGC 1 cut(s) 293
BccI CCATC 3 cut(s) 159, 305, 435
BciT130I CCWGG 2 cut(s) 173, 328
BfmI CTRYAG 1 cut(s) 112
BfoI RGCGCY 1 cut(s) 138
BisI GCNGC 4 cut(s) 158, 307, 361, 379
BlsI GCNGC 4 cut(s) 159, 308, 362, 380
Bme1390I CCNGG 2 cut(s) 173, 328
Bme18I GGWCC 2 cut(s) 219, 247
BmeT110I CYCGRG 2 cut(s) 7, 196
BmgT120I GGNCC 4 cut(s) 219, 247, 330, 362
BmrFI CCNGG 2 cut(s) 173, 328
BmsI GCATC 1 cut(s) 249
BpmI CTGGAG 1 cut(s) 194
BpuEI CTTGAG 2 cut(s) 199, 374
BsaBI GATNNNNATC 1 cut(s) 296
BsaHI GRCGYC 1 cut(s) 143
BsaJI CCNNGG 1 cut(s) 327
BsaXI ACNNNNNCTCC 6 cut(s) 50, 80, 167, 197, 357, 387
Bse1I ACTGG 1 cut(s) 250
Bse8I GATNNNNATC 1 cut(s) 296
BseBI CCWGG 2 cut(s) 173, 328
BseDI CCNNGG 1 cut(s) 327
BseGI GGATG 3 cut(s) 74, 224, 297
BseJI GATNNNNATC 1 cut(s) 296
BseNI ACTGG 1 cut(s) 250
BseRI GAGGAG 4 cut(s) 55, 58, 61, 220
BseX3I CGGCCG 1 cut(s) 155
BseXI GCAGC 1 cut(s) 293
Bsh1285I CGRYCG 1 cut(s) 158
BshFI GGCC 4 cut(s) 157, 326, 332, 363
BsiEI CGRYCG 1 cut(s) 158
BsiHKAI GWGCWC 1 cut(s) 168
BsiHKCI CYCGRG 2 cut(s) 7, 196
BsiSI CCGG 2 cut(s) 161, 282
BslFI GGGAC 3 cut(s) 26, 169, 238
BsmFI GGGAC 3 cut(s) 26, 169, 238
BsnI GGCC 4 cut(s) 157, 326, 332, 363
BsoBI CYCGRG 2 cut(s) 7, 196
Bsp1286I GDGCHC 1 cut(s) 168
Bsp143I GATC 2 cut(s) 23, 51
BspACI CCGC 4 cut(s) 158, 360, 376, 379
BspANI GGCC 4 cut(s) 157, 326, 332, 363
BspMAI CTGCAG 1 cut(s) 116
BspPI GGATC 1 cut(s) 18
BsrI ACTGG 1 cut(s) 250
BssECI CCNNGG 1 cut(s) 327
BssMI GATC 2 cut(s) 23, 51
BssNI GRCGYC 1 cut(s) 143
Bst2UI CCWGG 2 cut(s) 173, 328
Bst4CI ACNGT 1 cut(s) 247
Bst6I CTCTTC 1 cut(s) 238
BstACI GRCGYC 1 cut(s) 143
BstDEI CTNAG 1 cut(s) 48
BstF5I GGATG 3 cut(s) 74, 224, 297
BstH2I RGCGCY 1 cut(s) 138
BstHHI GCGC 1 cut(s) 137
BstKTI GATC 2 cut(s) 26, 54
BstMBI GATC 2 cut(s) 23, 51
BstMCI CGRYCG 1 cut(s) 158
BstMWI GCNNNNNNNGC 1 cut(s) 163
BstNI CCWGG 2 cut(s) 173, 328
BstSCI CCNGG 2 cut(s) 171, 326
BstSFI CTRYAG 1 cut(s) 112
BstV1I GCAGC 1 cut(s) 293
BstXI CCANNNNNNTGG 1 cut(s) 434
BstZI CGGCCG 1 cut(s) 155
BsuRI GGCC 4 cut(s) 157, 326, 332, 363
BtsCI GGATG 3 cut(s) 74, 224, 297
BtsI GCAGTG 1 cut(s) 421
BtsIMutI CAGTG 1 cut(s) 421
CfoI GCGC 1 cut(s) 137
Cfr13I GGNCC 4 cut(s) 219, 247, 330, 362
DdeI CTNAG 1 cut(s) 48
DpnI GATC 2 cut(s) 25, 53
DpnII GATC 2 cut(s) 23, 51
EaeI YGGCCR 1 cut(s) 155
EagI CGGCCG 1 cut(s) 155
Eam1104I CTCTTC 1 cut(s) 238
EarI CTCTTC 1 cut(s) 238
EciI GGCGGA 1 cut(s) 365
Ecl136II GAGCTC 1 cut(s) 166
EclXI CGGCCG 1 cut(s) 155
Eco24I GRGCYC 1 cut(s) 168
Eco32I GATATC 1 cut(s) 294
Eco47I GGWCC 2 cut(s) 219, 247
Eco52I CGGCCG 1 cut(s) 155
Eco53kI GAGCTC 1 cut(s) 166
Eco88I CYCGRG 2 cut(s) 7, 196
EcoICRI GAGCTC 1 cut(s) 166
EcoO109I RGGNCCY 1 cut(s) 219
EcoRII CCWGG 2 cut(s) 171, 326
EcoRV GATATC 1 cut(s) 294
EcoT38I GRGCYC 1 cut(s) 168
FaqI GGGAC 3 cut(s) 26, 169, 238
Fnu4HI GCNGC 4 cut(s) 158, 307, 361, 379
FokI GGATG 3 cut(s) 61, 211, 284
FriOI GRGCYC 1 cut(s) 168
Fsp4HI GCNGC 4 cut(s) 158, 307, 361, 379
GlaI GCGC 1 cut(s) 136
GluI GCNGC 4 cut(s) 158, 307, 361, 379
GsuI CTGGAG 1 cut(s) 194
HaeII RGCGCY 1 cut(s) 138
HaeIII GGCC 4 cut(s) 157, 326, 332, 363
HapII CCGG 2 cut(s) 161, 282
HhaI GCGC 1 cut(s) 137
Hin1I GRCGYC 1 cut(s) 143
Hin6I GCGC 1 cut(s) 135
HinP1I GCGC 1 cut(s) 135
HinfI GANTC 1 cut(s) 199
HpaII CCGG 2 cut(s) 161, 282
HphI GGTGA 2 cut(s) 95, 388
Hpy166II GTNNAC 1 cut(s) 396
Hpy8I GTNNAC 1 cut(s) 396
Hpy99I CGWCG 2 cut(s) 38, 41
HpyAV CCTTC 3 cut(s) 247, 361, 421
HpyCH4III ACNGT 1 cut(s) 247
HpyCH4IV ACGT 1 cut(s) 143
HpyCH4V TGCA 1 cut(s) 114
HpyF10VI GCNNNNNNNGC 1 cut(s) 163
HpyF3I CTNAG 1 cut(s) 48
HpySE526I ACGT 1 cut(s) 143
Hsp92I GRCGYC 1 cut(s) 143
HspAI GCGC 1 cut(s) 135
Kzo9I GATC 2 cut(s) 23, 51
LmnI GCTCC 3 cut(s) 163, 175, 378
Lsp1109I GCAGC 1 cut(s) 293
LweI GCATC 1 cut(s) 249
MaeII ACGT 1 cut(s) 143
MaeIII GTNAC 3 cut(s) 139, 144, 241
MalI GATC 2 cut(s) 25, 53
MboI GATC 2 cut(s) 23, 51
MboII GAAGA 1 cut(s) 225
MhlI GDGCHC 1 cut(s) 168
MlyI GAGTC 1 cut(s) 208
MseI TTAA 1 cut(s) 269
MspI CCGG 2 cut(s) 161, 282
MspR9I CCNGG 2 cut(s) 173, 328
MvaI CCWGG 2 cut(s) 173, 328
MwoI GCNNNNNNNGC 1 cut(s) 163
NdeII GATC 2 cut(s) 23, 51
NmuCI GTSAC 3 cut(s) 139, 144, 241
PaeR7I CTCGAG 2 cut(s) 7, 196
PkrI GCNGC 4 cut(s) 159, 308, 362, 380
PleI GAGTC 1 cut(s) 207
PpsI GAGTC 1 cut(s) 207
PpuMI RGGWCCY 1 cut(s) 219
Psp124BI GAGCTC 1 cut(s) 168
Psp5II RGGWCCY 1 cut(s) 219
Psp6I CCWGG 2 cut(s) 171, 326
PspGI CCWGG 2 cut(s) 171, 326
PspPI GGNCC 4 cut(s) 219, 247, 330, 362
PspPPI RGGWCCY 1 cut(s) 219
PspXI VCTCGAGB 2 cut(s) 7, 196
PstI CTGCAG 1 cut(s) 116
SacI GAGCTC 1 cut(s) 168
SaqAI TTAA 1 cut(s) 269
SatI GCNGC 4 cut(s) 158, 307, 361, 379
Sau3AI GATC 2 cut(s) 23, 51
Sau96I GGNCC 4 cut(s) 219, 247, 330, 362
SchI GAGTC 1 cut(s) 208
ScrFI CCNGG 2 cut(s) 173, 328
SduI GDGCHC 1 cut(s) 168
SfaNI GCATC 1 cut(s) 249
SfcI CTRYAG 1 cut(s) 112
Sfr274I CTCGAG 2 cut(s) 7, 196
SinI GGWCC 2 cut(s) 219, 247
SlaI CTCGAG 2 cut(s) 7, 196
SmlI CTYRAG 4 cut(s) 7, 196, 214, 389
SmoI CTYRAG 4 cut(s) 7, 196, 214, 389
SsiI CCGC 4 cut(s) 158, 360, 376, 379
SstI GAGCTC 1 cut(s) 168
StyD4I CCNGG 2 cut(s) 171, 326
TaaI ACNGT 1 cut(s) 247
TaiI ACGT 1 cut(s) 146
TaqI TCGA 4 cut(s) 8, 22, 168, 197
TauI GCSGC 3 cut(s) 160, 363, 381
Tru1I TTAA 1 cut(s) 269
Tru9I TTAA 1 cut(s) 269
TscAI CASTG 1 cut(s) 428
TseFI GTSAC 3 cut(s) 139, 144, 241
TseI GCWGC 1 cut(s) 306
Tsp45I GTSAC 3 cut(s) 139, 144, 241
TspDTI ATGAA 2 cut(s) 403, 433
TspGWI ACGGA 3 cut(s) 22, 290, 334
TspRI CASTG 1 cut(s) 428
VpaK11BI GGWCC 2 cut(s) 219, 247
XhoI CTCGAG 2 cut(s) 7, 196
ZraI GACGTC 1 cut(s) 144
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.