MD10G1129100.v1.1

Hippocampus abundant

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr10
OG0001419
Physical Location & Seq
Forward (+)
20967075 .. 20975405
8331 bp
Loading structure...
UTR
Exon/CDS
Intron
MD10G1129100.v1.1.491

Sequence Viewer

Length: 1368 bp
ATGGAGATGGAGGGCATTGGAGGACTGAGGCATCTGTTTATGACAGTGTTTTTGCATGGGTTTGCAAGCTTCATGGTGATTCCAGCCATAATTGATATCACTATGCTGGCTCTTTGCCCTGGTCGAGATGAGTGCTCCCTTGCCATTTACCTCTCTGGTTTTCAACAAGCGATGATAGGTTTGGGAACGGTGGTGATGACTCCACTAATTGGGAATCTGTCGGATGTGTATGGGAGGAAGTCTCTTCTCACGATCCCAATGACACTCTCCATTGTCCCACTAGCGATATTGGCGTATAGCAGGAAGACAAGCTTCTTTTATGCCTACTATGTGCTGAGGACAGTAACTGCCTTGGTAGGTGAAGGTAGCATCAACTGCCTCACCCTTGCTTATGTGGCCGACAATGTTTCAGAGAGAGAACGAATATCTGCATTTGGAATTCTTGCTGGATTTACCTCAGCAGCATTTGTATGTGGAACCTTAGCAGCTCGTTTCCTCTCCACTGCTTCAACATTTCAGGTTGCTGCCTTTGCATCAATGCTTTCAACTATCTACATGAGAATTTTTCTCAAGGAGAGCTTGCCGGGCGATGATAGTACTTTGAGGCAGCCTATCTTGAAAGGAGGAGTACCAGAAGTTGTTGATGAAGGTGATGTCTACTCGCCGAAAACAATACAAGTATTTAAGAAAATTCCATCTCCAGGAGATTTGATTTCCCTACTAAGGAGTAGTAAGATTCTGTCACAAGCAGCAGTTGTTTCCTTCTTTCATAGCCTGGCAGAGGGTGGCATGCAAGCTTCATTAATGTATTTCTTAAAGGCTCGTTTCCACTTCAACAAGAATCAGTTTGCTGATCTAATGCTGATTGTTGGGATATCAGGGACCATATCACAGGCTAGCAATACCCAAACCTTCTGCTGCTACAGTCTGCTTTTCATGCCTATGTTGGCAACTGCTATAGGAGAGGAAAAGTTGCTTGCAATTGGGCTCATAATGGGCAGTATGAATATGTTTCTTAACAGCATATCATGGGCAGTTTGGGTTCCTTATGCTGCGACTCTGTTTTCTGTTTTTGGATTTTTAGTGCAGCCAAGTATACGCAGCATCGCATCAAAACAAGTTGGACCCAATGATCAGGGAAAGGCTCAAGGGTGCATTTCAGGCATAAGCTCCTTTGCCAACATTGTTTCTCCATTAATTTTCAGTCCTCTAACAGCTTTATTCCTGTCTGAAGAAGCACCATTTTATTTCCCTGGCTTCAGTATTCTGTGCATTGGCCTTGCAACGATTATTGCCTTCGTTCAGAGCCTAATGATAAGGGCTTCTCCTCACAAAACCAGCACCAACATCTGCGTGGAATCTCCCTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
MFS_1

Protein Analysis

456

Amino Acids

49.13

Weight (kDa)

8.02

Isoelectric Point (pI)

36.8

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
MFS_1 PF07690 12 - 298 2.4e-22 Major Facilitator Superfamily
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 1 cut(s) 209
AccI GTMKAC 2 cut(s) 657, 1096
AclWI GGATC 1 cut(s) 247
AcoI YGGCCR 1 cut(s) 396
AcsI RAATTY 3 cut(s) 438, 561, 690
AcuI CTGAAG 2 cut(s) 1243, 1251
AfaI GTAC 2 cut(s) 598, 630
AfiI CCNNNNNNNGG 4 cut(s) 209, 701, 723, 781
AgsI TTSAA 5 cut(s) 164, 510, 546, 619, 835
AjnI CCWGG 4 cut(s) 118, 700, 774, 1252
AluBI AGCT 7 cut(s) 69, 312, 488, 579, 797, 1170, 1217
AluI AGCT 7 cut(s) 69, 312, 488, 579, 797, 1170, 1217
Alw21I GWGCWC 1 cut(s) 137
Alw26I GTCTC 1 cut(s) 246
AlwI GGATC 1 cut(s) 247
AlwNI CAGNNNCTG 1 cut(s) 347
AoxI GGCC 2 cut(s) 396, 1276
ApeKI GCWGC 9 cut(s) 461, 485, 524, 607, 749, 918, 1052, 1087, 1101
ApoI RAATTY 3 cut(s) 438, 561, 690
ArsI GACNNNNNNTTYG 2 cut(s) 34, 66
AseI ATTAAT 2 cut(s) 803, 1196
AspS9I GGNCC 2 cut(s) 882, 1124
AsuC2I CCSGG 1 cut(s) 585
AsuHPI GGTGA 5 cut(s) 88, 205, 371, 373, 662
AsuNHI GCTAGC 1 cut(s) 896
AvaII GGWCC 2 cut(s) 882, 1124
BanII GRGCYC 1 cut(s) 990
BbsI GAAGAC 1 cut(s) 311
Bbv12I GWGCWC 1 cut(s) 137
BbvCI CCTCAGC 2 cut(s) 335, 457
BbvI GCAGC 9 cut(s) 473, 497, 511, 619, 761, 905, 1039, 1099, 1113
BccI CCATC 1 cut(s) 703
BcgI CGANNNNNNTGC 2 cut(s) 114, 148
BciT130I CCWGG 4 cut(s) 120, 702, 776, 1254
BclI TGATCA 1 cut(s) 1132
BcnI CCSGG 1 cut(s) 585
BcoDI GTCTC 1 cut(s) 246
BfaI CTAG 3 cut(s) 281, 897, 1366
BfmI CTRYAG 2 cut(s) 922, 957
BisI GCNGC 9 cut(s) 462, 486, 525, 608, 750, 919, 1053, 1088, 1102
BlsI GCNGC 9 cut(s) 463, 487, 526, 609, 751, 920, 1054, 1089, 1103
BmcAI AGTACT 1 cut(s) 598
Bme1390I CCNGG 5 cut(s) 120, 585, 702, 776, 1254
Bme18I GGWCC 2 cut(s) 882, 1124
BmgT120I GGNCC 2 cut(s) 882, 1124
BmiI GGNNCC 4 cut(s) 478, 883, 1044, 1126
BmrFI CCNGG 5 cut(s) 120, 585, 702, 776, 1254
BmsI GCATC 5 cut(s) 40, 378, 542, 1113, 1118
BmtI GCTAGC 1 cut(s) 900
BpiI GAAGAC 1 cut(s) 311
BplI GAGNNNNNCTC 2 cut(s) 226, 258
BpmI CTGGAG 1 cut(s) 684
Bpu10I CCTNAGC 3 cut(s) 335, 457, 481
BpuEI CTTGAG 2 cut(s) 554, 1131
BpuMI CCSGG 1 cut(s) 585
BsaJI CCNNGG 3 cut(s) 118, 351, 1252
Bsc4I CCNNNNNNNGG 4 cut(s) 209, 701, 723, 781
BseBI CCWGG 4 cut(s) 120, 702, 776, 1254
BseDI CCNNGG 3 cut(s) 118, 351, 1252
BseGI GGATG 1 cut(s) 229
BseLI CCNNNNNNNGG 4 cut(s) 209, 701, 723, 781
BseMII CTCAG 3 cut(s) 17, 326, 471
BseRI GAGGAG 2 cut(s) 639, 1317
BseXI GCAGC 9 cut(s) 473, 497, 511, 619, 761, 905, 1039, 1099, 1113
BsgI GTGCAG 1 cut(s) 1106
BshFI GGCC 2 cut(s) 398, 1278
BsiHKAI GWGCWC 1 cut(s) 137
BsiSI CCGG 1 cut(s) 584
BslFI GGGAC 2 cut(s) 260, 895
BslI CCNNNNNNNGG 4 cut(s) 209, 701, 723, 781
BsmAI GTCTC 1 cut(s) 246
BsmFI GGGAC 2 cut(s) 260, 895
BsnI GGCC 2 cut(s) 398, 1278
Bsp1286I GDGCHC 2 cut(s) 137, 990
Bsp143I GATC 3 cut(s) 252, 853, 1132
BspANI GGCC 2 cut(s) 398, 1278
BspCNI CTCAG 3 cut(s) 18, 327, 470
BspLI GGNNCC 4 cut(s) 478, 883, 1044, 1126
BspOI GCTAGC 1 cut(s) 900
BspPI GGATC 1 cut(s) 247
BssECI CCNNGG 3 cut(s) 118, 351, 1252
BssMI GATC 3 cut(s) 252, 853, 1132
BssNAI GTATAC 1 cut(s) 1097
BssT1I CCWWGG 1 cut(s) 351
Bst1107I GTATAC 1 cut(s) 1097
Bst2UI CCWGG 4 cut(s) 120, 702, 776, 1254
Bst4CI ACNGT 4 cut(s) 46, 190, 343, 926
Bst6I CTCTTC 1 cut(s) 249
BstAPI GCANNNNNTGC 1 cut(s) 375
BstC8I GCNNGC 7 cut(s) 67, 108, 581, 791, 795, 898, 978
BstDEI CTNAG 5 cut(s) 26, 335, 457, 481, 722
BstENI CCTNNNNNAGG 1 cut(s) 779
BstF5I GGATG 1 cut(s) 229
BstKTI GATC 3 cut(s) 255, 856, 1135
BstMAI GTCTC 1 cut(s) 246
BstMBI GATC 3 cut(s) 252, 853, 1132
BstMWI GCNNNNNNNGC 7 cut(s) 290, 375, 395, 530, 585, 937, 1161
BstNI CCWGG 4 cut(s) 120, 702, 776, 1254
BstNSI RCATGY 1 cut(s) 793
BstSCI CCNGG 5 cut(s) 118, 583, 700, 774, 1252
BstSFI CTRYAG 2 cut(s) 922, 957
BstV1I GCAGC 9 cut(s) 473, 497, 511, 619, 761, 905, 1039, 1099, 1113
BstV2I GAAGAC 1 cut(s) 311
BstZ17I GTATAC 1 cut(s) 1097
BsuRI GGCC 2 cut(s) 398, 1278
BtgZI GCGATG 3 cut(s) 185, 603, 1090
BtsCI GGATG 1 cut(s) 229
BtsI GCAGTG 1 cut(s) 501
BtsIMutI CAGTG 2 cut(s) 51, 501
Cac8I GCNNGC 7 cut(s) 67, 108, 581, 791, 795, 898, 978
CaiI CAGNNNCTG 1 cut(s) 347
Cfr13I GGNCC 2 cut(s) 882, 1124
Csp6I GTAC 2 cut(s) 597, 629
CviAII CATG 6 cut(s) 56, 73, 556, 790, 937, 1029
CviQI GTAC 2 cut(s) 597, 629
DdeI CTNAG 5 cut(s) 26, 335, 457, 481, 722
DpnI GATC 3 cut(s) 254, 855, 1134
DpnII GATC 3 cut(s) 252, 853, 1132
EaeI YGGCCR 1 cut(s) 396
Eam1104I CTCTTC 1 cut(s) 249
EarI CTCTTC 1 cut(s) 249
Eco130I CCWWGG 1 cut(s) 351
Eco24I GRGCYC 1 cut(s) 990
Eco32I GATATC 2 cut(s) 97, 876
Eco47I GGWCC 2 cut(s) 882, 1124
Eco57I CTGAAG 2 cut(s) 1243, 1251
EcoNI CCTNNNNNAGG 1 cut(s) 779
EcoRI GAATTC 1 cut(s) 438
EcoRII CCWGG 4 cut(s) 118, 700, 774, 1252
EcoRV GATATC 2 cut(s) 97, 876
EcoT14I CCWWGG 1 cut(s) 351
EcoT38I GRGCYC 1 cut(s) 990
ErhI CCWWGG 1 cut(s) 351
FaeI CATG 6 cut(s) 59, 76, 559, 793, 940, 1032
FalI AAGNNNNNCTT 4 cut(s) 296, 328, 563, 595
FaqI GGGAC 2 cut(s) 260, 895
FatI CATG 6 cut(s) 55, 72, 555, 789, 936, 1028
FbaI TGATCA 1 cut(s) 1132
FblI GTMKAC 2 cut(s) 657, 1096
Fnu4HI GCNGC 9 cut(s) 462, 486, 525, 608, 750, 919, 1053, 1088, 1102
FokI GGATG 1 cut(s) 236
FriOI GRGCYC 1 cut(s) 990
Fsp4HI GCNGC 9 cut(s) 462, 486, 525, 608, 750, 919, 1053, 1088, 1102
FspBI CTAG 3 cut(s) 281, 897, 1366
GluI GCNGC 9 cut(s) 462, 486, 525, 608, 750, 919, 1053, 1088, 1102
GsuI CTGGAG 1 cut(s) 684
HaeIII GGCC 2 cut(s) 398, 1278
HapII CCGG 1 cut(s) 584
Hin1II CATG 6 cut(s) 59, 76, 559, 793, 940, 1032
HindIII AAGCTT 3 cut(s) 67, 310, 795
HinfI GANTC 7 cut(s) 79, 199, 214, 736, 841, 1057, 1358
HpaII CCGG 1 cut(s) 584
HphI GGTGA 5 cut(s) 88, 205, 371, 373, 662
Hpy166II GTNNAC 2 cut(s) 658, 1097
Hpy188I TCNGA 4 cut(s) 223, 412, 1231, 1305
Hpy188III TCNNGA 3 cut(s) 125, 250, 616
Hpy8I GTNNAC 2 cut(s) 658, 1097
HpyAV CCTTC 5 cut(s) 356, 641, 772, 922, 1306
HpyCH4III ACNGT 4 cut(s) 46, 190, 343, 926
HpyF10VI GCNNNNNNNGC 7 cut(s) 290, 375, 395, 530, 585, 937, 1161
HpyF3I CTNAG 5 cut(s) 26, 335, 457, 481, 722
Hsp92II CATG 6 cut(s) 59, 76, 559, 793, 940, 1032
Ksp22I TGATCA 1 cut(s) 1132
Kzo9I GATC 3 cut(s) 252, 853, 1132
LmnI GCTCC 2 cut(s) 140, 1175
Lsp1109I GCAGC 9 cut(s) 473, 497, 511, 619, 761, 905, 1039, 1099, 1113
LweI GCATC 5 cut(s) 40, 378, 542, 1113, 1118
MaeI CTAG 3 cut(s) 281, 897, 1366
MaeIII GTNAC 2 cut(s) 343, 741
MalI GATC 3 cut(s) 254, 855, 1134
MboI GATC 3 cut(s) 252, 853, 1132
MboII GAAGA 3 cut(s) 236, 316, 1244
MfeI CAATTG 1 cut(s) 981
MhlI GDGCHC 2 cut(s) 137, 990
MluCI AATT 7 cut(s) 90, 207, 438, 561, 690, 981, 1197
MlyI GAGTC 2 cut(s) 193, 1051
MmeI TCCRAC 2 cut(s) 201, 1102
MseI TTAA 5 cut(s) 684, 803, 815, 1017, 1196
MslI CAYNNNNRTG 3 cut(s) 469, 941, 1352
MspI CCGG 1 cut(s) 584
MspR9I CCNGG 5 cut(s) 120, 585, 702, 776, 1254
MunI CAATTG 1 cut(s) 981
MvaI CCWGG 4 cut(s) 120, 702, 776, 1254
MwoI GCNNNNNNNGC 7 cut(s) 290, 375, 395, 530, 585, 937, 1161
NciI CCSGG 1 cut(s) 585
NdeII GATC 3 cut(s) 252, 853, 1132
NheI GCTAGC 1 cut(s) 896
NlaIII CATG 6 cut(s) 59, 76, 559, 793, 940, 1032
NlaIV GGNNCC 4 cut(s) 478, 883, 1044, 1126
NmuCI GTSAC 1 cut(s) 741
NspI RCATGY 1 cut(s) 793
PaeI GCATGC 1 cut(s) 793
PfeI GAWTC 5 cut(s) 79, 214, 736, 841, 1358
PflMI CCANNNNNTGG 1 cut(s) 209
PfoI TCCNGGA 1 cut(s) 700
PkrI GCNGC 9 cut(s) 463, 487, 526, 609, 751, 920, 1054, 1089, 1103
PleI GAGTC 2 cut(s) 193, 1051
PpsI GAGTC 2 cut(s) 193, 1051
PshBI ATTAAT 2 cut(s) 803, 1196
Psp6I CCWGG 4 cut(s) 118, 700, 774, 1252
PspGI CCWGG 4 cut(s) 118, 700, 774, 1252
PspN4I GGNNCC 4 cut(s) 478, 883, 1044, 1126
PspPI GGNCC 2 cut(s) 882, 1124
PstNI CAGNNNCTG 1 cut(s) 347
RsaI GTAC 2 cut(s) 598, 630
RsaNI GTAC 2 cut(s) 597, 629
RseI CAYNNNNRTG 3 cut(s) 469, 941, 1352
SaqAI TTAA 5 cut(s) 684, 803, 815, 1017, 1196
SatI GCNGC 9 cut(s) 462, 486, 525, 608, 750, 919, 1053, 1088, 1102
Sau3AI GATC 3 cut(s) 252, 853, 1132
Sau96I GGNCC 2 cut(s) 882, 1124
ScaI AGTACT 1 cut(s) 598
SchI GAGTC 2 cut(s) 193, 1051
ScrFI CCNGG 5 cut(s) 120, 585, 702, 776, 1254
SduI GDGCHC 2 cut(s) 137, 990
SfaNI GCATC 5 cut(s) 40, 378, 542, 1113, 1118
SfcI CTRYAG 2 cut(s) 922, 957
SinI GGWCC 2 cut(s) 882, 1124
SmiMI CAYNNNNRTG 3 cut(s) 469, 941, 1352
SmlI CTYRAG 2 cut(s) 569, 1146
SmoI CTYRAG 2 cut(s) 569, 1146
SphI GCATGC 1 cut(s) 793
Sse9I AATT 7 cut(s) 90, 207, 438, 561, 690, 981, 1197
SspMI CTAG 3 cut(s) 281, 897, 1366
StyD4I CCNGG 5 cut(s) 118, 583, 700, 774, 1252
StyI CCWWGG 1 cut(s) 351
TaaI ACNGT 4 cut(s) 46, 190, 343, 926
TaqI TCGA 1 cut(s) 124
TasI AATT 7 cut(s) 90, 207, 438, 561, 690, 981, 1197
TatI WGTACW 1 cut(s) 596
TfiI GAWTC 5 cut(s) 79, 214, 736, 841, 1358
Tru1I TTAA 5 cut(s) 684, 803, 815, 1017, 1196
Tru9I TTAA 5 cut(s) 684, 803, 815, 1017, 1196
TscAI CASTG 2 cut(s) 51, 508
TseFI GTSAC 1 cut(s) 741
TseI GCWGC 9 cut(s) 461, 485, 524, 607, 749, 918, 1052, 1087, 1101
Tsp45I GTSAC 1 cut(s) 741
TspDTI ATGAA 6 cut(s) 61, 660, 758, 789, 925, 1019
TspRI CASTG 2 cut(s) 51, 508
Van91I CCANNNNNTGG 1 cut(s) 209
VpaK11BI GGWCC 2 cut(s) 882, 1124
VspI ATTAAT 2 cut(s) 803, 1196
XagI CCTNNNNNAGG 1 cut(s) 779
XapI RAATTY 3 cut(s) 438, 561, 690
XceI RCATGY 1 cut(s) 793
XcmI CCANNNNNNNNNTGG 1 cut(s) 1351
XmiI GTMKAC 2 cut(s) 657, 1096
XspI CTAG 3 cut(s) 281, 897, 1366
ZrmI AGTACT 1 cut(s) 598
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.