MD10G1168200.v1.1

PB1 domain

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr10
OG0004019
Physical Location & Seq
Reverse (-)
26012565 .. 26013288
724 bp
Loading structure...
UTR
Exon/CDS
Intron
MD10G1168200.v1.1.491

Sequence Viewer

Length: 366 bp
ATGATCTGGTACATATGGAACTGTTTATCATGGAAAATGGCGGGGAACAGATGTTGCTATAAAGCGAATAAAAAAGAGCTGTTTTGCAGGAAGATCATCAGAGCAAGACCGACTGGTGGGTTTCCTGAACAAATTTGTTTTACAAAGGACTTCTGGAGAGAGGCGCAAATCCTTTCAGCTCTCCATCATCCAAATGTGGTTGCATTATATGGGGTAGTACCTGATGGAGCTGGAGGAACGTTGGCAACTGTAACTGAGTACATGGTGAACGGATCACTTAGGCATGCCCTACTTAAAAAGAATAGATCACTTGACCGTCGTAGAAAGCTTATAATTGCCATGGATGCTGCTTTTGGCATGGAATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0001666 GO:0003674 GO:0003824 GO:0004672 GO:0004674 GO:0004712 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005783 GO:0005789 GO:0006082 GO:0006464 GO:0006468 GO:0006629 GO:0006720 GO:0006721 GO:0006793 GO:0006796 GO:0006807 GO:0006950 GO:0007154 GO:0007165 GO:0008150 GO:0008152 GO:0008299 GO:0008610 GO:0009058 GO:0009628 GO:0009685 GO:0009686 GO:0009719 GO:0009723 GO:0009725 GO:0009743 GO:0009744 GO:0009746 GO:0009750 GO:0009756 GO:0009966 GO:0009968 GO:0009987 GO:0010033 GO:0010035 GO:0010038 GO:0010039 GO:0010104 GO:0010105 GO:0010182 GO:0010646 GO:0010648 GO:0010817 GO:0012505 GO:0016020 GO:0016053 GO:0016101 GO:0016102 GO:0016114 GO:0016301 GO:0016310 GO:0016740 GO:0016772 GO:0016773 GO:0019538 GO:0019752 GO:0023051 GO:0023052 GO:0023057 GO:0031984 GO:0034284 GO:0034285 GO:0036211 GO:0036293 GO:0040034 GO:0042175 GO:0042221 GO:0042445 GO:0042446 GO:0043170 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043412 GO:0043436 GO:0044237 GO:0044238 GO:0044249 GO:0044255 GO:0044260 GO:0044267 GO:0044281 GO:0044283 GO:0044422 GO:0044424 GO:0044425 GO:0044432 GO:0044444 GO:0044446 GO:0044464 GO:0046394 GO:0046777 GO:0048506 GO:0048509 GO:0048510 GO:0048519 GO:0048523 GO:0048580 GO:0048583 GO:0048585 GO:0050789 GO:0050793 GO:0050794 GO:0050896 GO:0051239 GO:0051302 GO:0051716 GO:0065007 GO:0065008 GO:0070297 GO:0070298 GO:0070482 GO:0070887 GO:0071241 GO:0071248 GO:0071281 GO:0071310 GO:0071322 GO:0071704 GO:0098827 GO:0140096 GO:1901564 GO:1901576 GO:1901700 GO:1901701 GO:1902531 GO:1902532 GO:2000026 GO:2000035 GO:2000069 GO:2000280
KEGG Pathways
Metabolic & Signaling
ko04010 map04010
Pfam Domains
Protein Families
PB1 Pkinase_Tyr

Protein Analysis

122

Amino Acids

13.85

Weight (kDa)

9.75

Isoelectric Point (pI)

41.24

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Pkinase PF00069 19 - 102 9.6e-08 Protein kinase domain
PK_Tyr_Ser-Thr PF07714 48 - 121 6.3e-13 Protein tyrosine and serine/threonine kinase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 332
AciI CCGC 1 cut(s) 41
AclI AACGTT 1 cut(s) 239
AclWI GGATC 1 cut(s) 280
AcsI RAATTY 1 cut(s) 132
AfaI GTAC 3 cut(s) 11, 219, 260
AfiI CCNNNNNNNGG 1 cut(s) 116
AluBI AGCT 4 cut(s) 79, 179, 230, 328
AluI AGCT 4 cut(s) 79, 179, 230, 328
AlwI GGATC 1 cut(s) 280
ApeKI GCWGC 1 cut(s) 347
ApoI RAATTY 1 cut(s) 132
AspLEI GCGC 1 cut(s) 166
AsuHPI GGTGA 1 cut(s) 277
BbvI GCAGC 1 cut(s) 334
BccI CCATC 2 cut(s) 192, 218
BisI GCNGC 1 cut(s) 348
BlsI GCNGC 1 cut(s) 349
BmsI GCATC 1 cut(s) 334
BpmI CTGGAG 2 cut(s) 175, 252
BsaJI CCNNGG 1 cut(s) 339
Bsc4I CCNNNNNNNGG 1 cut(s) 116
Bse1I ACTGG 1 cut(s) 118
BseDI CCNNGG 1 cut(s) 339
BseGI GGATG 2 cut(s) 187, 349
BseLI CCNNNNNNNGG 1 cut(s) 116
BseMII CTCAG 1 cut(s) 246
BseNI ACTGG 1 cut(s) 118
BseXI GCAGC 1 cut(s) 334
BslI CCNNNNNNNGG 1 cut(s) 116
Bsp143I GATC 4 cut(s) 3, 93, 272, 305
Bsp19I CCATGG 1 cut(s) 339
BspACI CCGC 1 cut(s) 41
BspCNI CTCAG 1 cut(s) 247
BspPI GGATC 1 cut(s) 280
BsrI ACTGG 1 cut(s) 118
BssECI CCNNGG 1 cut(s) 339
BssMI GATC 4 cut(s) 3, 93, 272, 305
BssT1I CCWWGG 1 cut(s) 339
Bst4CI ACNGT 3 cut(s) 23, 250, 317
BstC8I GCNNGC 1 cut(s) 285
BstDEI CTNAG 2 cut(s) 255, 278
BstDSI CCRYGG 1 cut(s) 339
BstF5I GGATG 2 cut(s) 187, 349
BstHHI GCGC 1 cut(s) 166
BstKTI GATC 4 cut(s) 6, 96, 275, 308
BstMBI GATC 4 cut(s) 3, 93, 272, 305
BstMWI GCNNNNNNNGC 1 cut(s) 344
BstNSI RCATGY 1 cut(s) 287
BstV1I GCAGC 1 cut(s) 334
BtgI CCRYGG 1 cut(s) 339
BtsCI GGATG 2 cut(s) 187, 349
Cac8I GCNNGC 1 cut(s) 285
CfoI GCGC 1 cut(s) 166
Csp6I GTAC 3 cut(s) 10, 218, 259
CviAII CATG 5 cut(s) 30, 262, 284, 340, 358
CviJI RGCY 4 cut(s) 79, 179, 230, 328
CviKI_1 RGCY 4 cut(s) 79, 179, 230, 328
CviQI GTAC 3 cut(s) 10, 218, 259
DdeI CTNAG 2 cut(s) 255, 278
DpnI GATC 4 cut(s) 5, 95, 274, 307
DpnII GATC 4 cut(s) 3, 93, 272, 305
Eco130I CCWWGG 1 cut(s) 339
EcoT14I CCWWGG 1 cut(s) 339
ErhI CCWWGG 1 cut(s) 339
FaeI CATG 5 cut(s) 33, 265, 287, 343, 361
FatI CATG 5 cut(s) 29, 261, 283, 339, 357
FauI CCCGC 1 cut(s) 34
FauNDI CATATG 1 cut(s) 14
Fnu4HI GCNGC 1 cut(s) 348
FokI GGATG 2 cut(s) 174, 356
Fsp4HI GCNGC 1 cut(s) 348
GlaI GCGC 1 cut(s) 165
GluI GCNGC 1 cut(s) 348
GsuI CTGGAG 2 cut(s) 175, 252
HhaI GCGC 1 cut(s) 166
Hin1II CATG 5 cut(s) 33, 265, 287, 343, 361
Hin6I GCGC 1 cut(s) 164
HinP1I GCGC 1 cut(s) 164
HindIII AAGCTT 1 cut(s) 326
HphI GGTGA 1 cut(s) 277
Hpy166II GTNNAC 1 cut(s) 268
Hpy188I TCNGA 1 cut(s) 101
Hpy188III TCNNGA 2 cut(s) 125, 154
Hpy8I GTNNAC 1 cut(s) 268
Hpy99I CGWCG 1 cut(s) 321
HpyCH4III ACNGT 3 cut(s) 23, 250, 317
HpyCH4IV ACGT 1 cut(s) 239
HpyCH4V TGCA 2 cut(s) 87, 203
HpyF10VI GCNNNNNNNGC 1 cut(s) 344
HpyF3I CTNAG 2 cut(s) 255, 278
HpySE526I ACGT 1 cut(s) 239
Hsp92II CATG 5 cut(s) 33, 265, 287, 343, 361
HspAI GCGC 1 cut(s) 164
Kzo9I GATC 4 cut(s) 3, 93, 272, 305
LmnI GCTCC 1 cut(s) 227
LpnPI CCDG 6 cut(s) 73, 99, 138, 139, 216, 234
Lsp1109I GCAGC 1 cut(s) 334
LweI GCATC 1 cut(s) 334
MaeII ACGT 1 cut(s) 239
MaeIII GTNAC 1 cut(s) 250
MalI GATC 4 cut(s) 5, 95, 274, 307
MboI GATC 4 cut(s) 3, 93, 272, 305
MboII GAAGA 1 cut(s) 103
MluCI AATT 2 cut(s) 132, 333
MnlI CCTC 2 cut(s) 154, 227
MseI TTAA 1 cut(s) 294
MslI CAYNNNNRTG 1 cut(s) 192
MwoI GCNNNNNNNGC 1 cut(s) 344
NcoI CCATGG 1 cut(s) 339
NdeI CATATG 1 cut(s) 14
NdeII GATC 4 cut(s) 3, 93, 272, 305
NlaIII CATG 5 cut(s) 33, 265, 287, 343, 361
NspI RCATGY 1 cut(s) 287
PaeI GCATGC 1 cut(s) 287
PkrI GCNGC 1 cut(s) 349
PsiI TTATAA 1 cut(s) 332
Psp1406I AACGTT 1 cut(s) 239
RsaI GTAC 3 cut(s) 11, 219, 260
RsaNI GTAC 3 cut(s) 10, 218, 259
RseI CAYNNNNRTG 1 cut(s) 192
SaqAI TTAA 1 cut(s) 294
SatI GCNGC 1 cut(s) 348
Sau3AI GATC 4 cut(s) 3, 93, 272, 305
SetI ASST 6 cut(s) 81, 181, 223, 232, 242, 330
SfaNI GCATC 1 cut(s) 334
SmiMI CAYNNNNRTG 1 cut(s) 192
SphI GCATGC 1 cut(s) 287
Sse9I AATT 2 cut(s) 132, 333
SsiI CCGC 1 cut(s) 41
StyI CCWWGG 1 cut(s) 339
TaaI ACNGT 3 cut(s) 23, 250, 317
TaiI ACGT 1 cut(s) 242
TaqII GACCGA 1 cut(s) 124
TasI AATT 2 cut(s) 132, 333
TatI WGTACW 1 cut(s) 258
Tru1I TTAA 1 cut(s) 294
Tru9I TTAA 1 cut(s) 294
TseI GCWGC 1 cut(s) 347
TspGWI ACGGA 1 cut(s) 285
XapI RAATTY 1 cut(s) 132
XceI RCATGY 1 cut(s) 287
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.