pycom10g19340

PB1 domain

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr10
OG0004019
Physical Location & Seq
Forward (+)
22462001 .. 22464577
2577 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom10g19340.1

Sequence Viewer

Length: 2577 bp
ATGCCCACGTGTCATATTTACTGTTTTTTTGCTTGGATGTTTATCCATGTGAATTTAGACTGCATGGATAGAAATCTGGGTAAAGGTACAATGGATCGACATAAAAACTATGAGCGTGTACGCTACAATAATGTTGAAGCTAGAAACGACGGAAATGGGTCATCCAATCAAAGGTACTTCCAGGATCCATCAACTAATGCGAATACTAACATGCGACCTCCAGATTACAGTGTTGCTGTTGGAGCCAGGCCTGTGTTAAATTACTCCATTCAGACTGGTGAGGAATTTGCTCTTGAATTCATGAGGGAAAGGGTGAACCCAAGGCAGCATTTAGCTTCTGGGGATCCTAATAGTGCGCCTAGCTATATGGATCTAAAAGGCATACTGGGAATTAGCCATACAGGTTCAGAAAGTGGGTCTGATACTTCGTTGCTGAACTCGGTAGATAAAGGGCGTGCCCAGGAATCCGAAAGAAAGGCATCTTATGCACATGAAGATAAGAGTTACTATGATTCGGTACAATTACCACAAACCTCATCAAGAAATGACAGCAATCGAGGACTTCATTATGCTTCTTCAGGAATGTCTGATAGCTCAGTGAGGAAAGTGAAGTTTCTCTGCAGCTTTGGTGGTAAAATTTTGCCGCGTCCGAGTGATGGAAGGCTTAGATACGTTGGAGGTGAAACACGTATTATACGGTTAAACAGGGATATCTTTTGGCAGGATCGGATCCAGAAAATGTTGACAATTTATGATGAAACTCGTGCAATAAAGTATCAGCTTCCTGGTGAGGATCTTGATGCACTTGTTTCTGTTTCATGTGATGAAGATCTGCAAAACATGATGGATGAATGCAATGTGCTACAAGACGGGGGATCACAGAAACCTAGGATGTTCCTGTTTTCTCATGTTGATTTGGAGGACTCTCAGTATGGTGTAGAAAGCATGGACGGTGATTCTGAGGTTCAGTATGTAGTTGCTGTAAATGGTATTGACTTACGTTCAAGAAAGAATTCTATTGCTTTGGCAAGTTCTTCAGGCAACAACTTGGAAGAGTTGCTCAGTCTAAATGTTGTAAGGGAGAGCACTCGAGCAGTGCCTGATACAGCTAGTGCTGGCGCAGCACCCTCAGCACCAAATGTGCCTTCCGCTAATCAATCTTCTCAATCAGTACTTCCAGGTTCTTCAAGGGCTTATGAATCCAATTCACAACCATACCAGGGGCAGAAGGTGCATAGTGTGGAAGCCAGACAGCATCCAGTGTCAACTTTCCACCCTGTCCCTGGGAAAGATGGTCTGACTCATGTGCCTTCATCGGTTCCATTGCAATATGATTCTGGTTCTCACCCTTCTCACTATGCAACACCTGGAGGAAATATAGATTCAATGCCTGTATACGGTCAATCCACTCAACAGGGGGGATTGATTGAAGAGCAGCTATGTGGTGGCATGCATGGGCAAGGTTCAGAATTGCCCATAAAGGAGATGAAATTGAAAAGAAACAGCTCTGCACAAAAGATAAATGAACCTGAGAAAATTCGATCCTCAGAGAAGGAAGCTCCACCAAAGGAAGCAAGAATGAAGAGAGAGAGCTCTCTCCAGAAGATAAATGAAAGCAATAAGCCTAGGAATTTAGTAAATGACAACACTGTTTCTCTACCTCCATCTGACAGTTCAGTTCCCAATCATATTTCTAGGGATGAAGTTTCAGTTGCCAATTCAGCTGCAGAGACAGGGTCACCTTTGTTGGCTACAAGAAGCAGCAAAAAGCTCCAGGAACCTCGACAAAATCCAATGACCTCTGAAGATGTAAATGATGGGAAAAAGATCAATGAAGATGACCGATTTGACACAGCCAGTGGACTGTCTAATCCTGGATACGGTGGCTCTGAGGTTGATTCTAGATATGGTGGCTCTGAGGTTGATTCTATGGACTTTAGCTACCTTGACCCGCCTGTGGCTCCTCAGCGTGTCTATCATTCTGAGCGGATCGTCAGGGAACAAGCAGAATTGAACCGTTTATCAAAATCTGGTGATTCTTATGGTTCCCAGTTTATGGTAACTCAAGCACAGTCCGATCACTCACTACCAATCACGGACTCTTTGGATAAATTGCATGGTGAGAATGTCCCCTTGCAGCCTGAGCAGTCTGTGCAGCCTGGCTTACCTTCAAAACTATTGCACGTTGAAGATGGACTGGCACAATTTGGAAAGTATAAAGAATTTGCTGAAAATATTAGTAAAATGAGTTCTGATGCTTATCATGAGCGGCTTGAGTCAAAGGTACAAAAATCGGATCAGGAAATGGGCCGGCCAAAAGATAACTACAAAGACCCAAGTAACAAAGATAAAGAAGCGGCAGTGCTGACTCAACAAACTGCTGATCCGGAAACATTTGGGAAGCTTACACATGATTCTGCTTCAGTTCCACCTGAATTTAAATGGTCTGAGATTGCTGGCAGTAAGGACAATGAGAATCATGCCAAGGGTCATGGACAACCATTGGCTAGGGCAGAGAACCCCAGAGGTGTTGCTCATGGGGAGTCTGCTGCTGGTGCTGGCACTCCTGAGCAGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
PB1 Pkinase_Tyr

Protein Analysis

859

Amino Acids

94.34

Weight (kDa)

5.57

Isoelectric Point (pI)

53.11

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PB1 PF00564 219 - 301 1.4e-14 PB1 domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 1 cut(s) 2056
AccBSI CCGCTC 2 cut(s) 1987, 2269
AccI GTMKAC 1 cut(s) 1395
AccII CGCG 1 cut(s) 646
AccIII TCCGGA 1 cut(s) 2386
AciI CCGC 6 cut(s) 644, 1149, 1952, 1987, 2269, 2357
AcoI YGGCCR 1 cut(s) 2312
AcsI RAATTY 9 cut(s) 52, 284, 296, 636, 1012, 1536, 1630, 2222, 2435
AcuI CTGAAG 4 cut(s) 561, 1020, 1824, 2406
AcvI CACGTG 1 cut(s) 9
AfaI GTAC 6 cut(s) 88, 120, 176, 519, 1173, 2286
AfiI CCNNNNNNNGG 8 cut(s) 171, 656, 1220, 1283, 1397, 1880, 1957, 2056
AflIII ACRYGT 2 cut(s) 8, 686
AjuI GAANNNNNNNTTGG 2 cut(s) 1129, 1161
Alw21I GWGCWC 2 cut(s) 1088, 1595
Alw26I GTCTC 1 cut(s) 1724
AlwNI CAGNNNCTG 1 cut(s) 1100
Ama87I CYCGRG 1 cut(s) 1089
Aor13HI TCCGGA 1 cut(s) 2386
AoxI GGCC 3 cut(s) 248, 2308, 2312
ApeKI GCWGC 9 cut(s) 325, 621, 1121, 1435, 1724, 1761, 2137, 2155, 2549
ApoI RAATTY 9 cut(s) 52, 284, 296, 636, 1012, 1536, 1630, 2222, 2435
Asp700I GAANNNNTTC 1 cut(s) 1012
AspA2I CCTAGG 2 cut(s) 887, 1625
AspLEI GCGC 2 cut(s) 358, 1121
AspS9I GGNCC 1 cut(s) 2308
AsuHPI GGTGA 9 cut(s) 290, 325, 692, 800, 965, 1337, 1731, 2045, 2132
AvaI CYCGRG 1 cut(s) 1089
AvrII CCTAGG 2 cut(s) 887, 1625
BaeGI GKGCMC 1 cut(s) 460
BamHI GGATCC 3 cut(s) 184, 343, 729
BanII GRGCYC 1 cut(s) 1595
BauI CACGAG 1 cut(s) 762
BbrPI CACGTG 1 cut(s) 9
Bbv12I GWGCWC 2 cut(s) 1088, 1595
BbvCI CCTCAGC 2 cut(s) 1129, 1965
BbvI GCAGC 9 cut(s) 337, 633, 1133, 1447, 1711, 1773, 2149, 2167, 2536
BccI CCATC 7 cut(s) 196, 650, 838, 1286, 1672, 1811, 2186
BciVI GTATCC 1 cut(s) 1871
BcoDI GTCTC 1 cut(s) 1724
BfaI CTAG 8 cut(s) 141, 360, 888, 1110, 1626, 1695, 1902, 2508
BfmI CTRYAG 2 cut(s) 619, 1725
BfuI GTATCC 1 cut(s) 1871
BglII AGATCT 1 cut(s) 829
BlnI CCTAGG 2 cut(s) 887, 1625
BmcAI AGTACT 1 cut(s) 1173
BmeT110I CYCGRG 1 cut(s) 1089
BmgT120I GGNCC 1 cut(s) 2308
BmiI GGNNCC 8 cut(s) 186, 244, 345, 731, 1320, 1779, 1962, 2047
BmrI ACTGGG 2 cut(s) 395, 2044
BmsI GCATC 4 cut(s) 488, 790, 1264, 2245
BmuI ACTGGG 2 cut(s) 395, 2044
BpmI CTGGAG 4 cut(s) 204, 1389, 1583, 1757
Bpu10I CCTNAGC 4 cut(s) 1129, 1965, 2142, 2568
BpuEI CTTGAG 2 cut(s) 2049, 2294
BsaAI YACGTR 2 cut(s) 9, 689
BsaBI GATNNNNATC 4 cut(s) 41, 72, 828, 2259
BsaJI CCNNGG 8 cut(s) 320, 459, 887, 1219, 1282, 1283, 1625, 2484
BsaWI WCCGGW 1 cut(s) 2386
Bsc4I CCNNNNNNNGG 8 cut(s) 171, 656, 1220, 1283, 1397, 1880, 1957, 2056
Bse118I RCCGGY 1 cut(s) 2310
Bse1I ACTGG 6 cut(s) 280, 390, 1259, 1857, 2050, 2202
Bse3DI GCAATG 2 cut(s) 862, 1322
Bse8I GATNNNNATC 4 cut(s) 41, 72, 828, 2259
BseAI TCCGGA 1 cut(s) 2386
BseDI CCNNGG 8 cut(s) 320, 459, 887, 1219, 1282, 1283, 1625, 2484
BseGI GGATG 6 cut(s) 42, 161, 853, 897, 1255, 1705
BseJI GATNNNNATC 4 cut(s) 41, 72, 828, 2259
BseLI CCNNNNNNNGG 8 cut(s) 171, 656, 1220, 1283, 1397, 1880, 1957, 2056
BseMI GCAATG 2 cut(s) 862, 1322
BseNI ACTGG 6 cut(s) 280, 390, 1259, 1857, 2050, 2202
BseRI GAGGAG 1 cut(s) 1953
BseSI GKGCMC 1 cut(s) 460
BseXI GCAGC 9 cut(s) 337, 633, 1133, 1447, 1711, 1773, 2149, 2167, 2536
BsgI GTGCAG 2 cut(s) 1494, 2174
Bsh1236I CGCG 1 cut(s) 646
BshFI GGCC 3 cut(s) 250, 2310, 2314
BsiHKAI GWGCWC 2 cut(s) 1088, 1595
BsiHKCI CYCGRG 1 cut(s) 1089
BsiSI CCGG 2 cut(s) 2311, 2387
BslFI GGGAC 2 cut(s) 1265, 2114
BslI CCNNNNNNNGG 8 cut(s) 171, 656, 1220, 1283, 1397, 1880, 1957, 2056
BsmAI GTCTC 1 cut(s) 1724
BsmFI GGGAC 2 cut(s) 1265, 2114
BsmI GAATGC 1 cut(s) 857
BsnI GGCC 3 cut(s) 250, 2310, 2314
BsoBI CYCGRG 1 cut(s) 1089
Bsp1286I GDGCHC 3 cut(s) 460, 1088, 1595
Bsp13I TCCGGA 1 cut(s) 2386
BspACI CCGC 6 cut(s) 644, 1149, 1952, 1987, 2269, 2357
BspANI GGCC 3 cut(s) 250, 2310, 2314
BspEI TCCGGA 1 cut(s) 2386
BspFNI CGCG 1 cut(s) 646
BspHI TCATGA 2 cut(s) 300, 2263
BspLI GGNNCC 8 cut(s) 186, 244, 345, 731, 1320, 1779, 1962, 2047
BspMAI CTGCAG 2 cut(s) 623, 1729
BspQI GCTCTTC 1 cut(s) 1425
BsrBI CCGCTC 2 cut(s) 1987, 2269
BsrDI GCAATG 2 cut(s) 862, 1322
BsrFI RCCGGY 1 cut(s) 2310
BsrI ACTGG 6 cut(s) 280, 390, 1259, 1857, 2050, 2202
BssAI RCCGGY 1 cut(s) 2310
BssECI CCNNGG 8 cut(s) 320, 459, 887, 1219, 1282, 1283, 1625, 2484
BssNAI GTATAC 1 cut(s) 1396
BssSI CACGAG 1 cut(s) 762
BssT1I CCWWGG 4 cut(s) 320, 887, 1625, 2484
Bst1107I GTATAC 1 cut(s) 1396
Bst2BI CACGAG 1 cut(s) 762
Bst6I CTCTTC 3 cut(s) 1047, 1425, 1577
BstAPI GCANNNNNTGC 3 cut(s) 485, 1231, 2152
BstBAI YACGTR 2 cut(s) 9, 689
BstC8I GCNNGC 6 cut(s) 456, 1117, 1451, 2312, 2458, 2560
BstEII GGTNACC 1 cut(s) 1737
BstF5I GGATG 6 cut(s) 42, 161, 853, 897, 1255, 1705
BstFNI CGCG 1 cut(s) 646
BstHHI GCGC 2 cut(s) 358, 1121
BstMAI GTCTC 1 cut(s) 1724
BstMWI GCNNNNNNNGC 9 cut(s) 242, 485, 1121, 1130, 1231, 1721, 2143, 2152, 2555
BstNSI RCATGY 2 cut(s) 214, 1453
BstPI GGTNACC 1 cut(s) 1737
BstSFI CTRYAG 2 cut(s) 619, 1725
BstSLI GKGCMC 1 cut(s) 460
BstUI CGCG 1 cut(s) 646
BstV1I GCAGC 9 cut(s) 337, 633, 1133, 1447, 1711, 1773, 2149, 2167, 2536
BstX2I RGATCY 6 cut(s) 184, 343, 370, 729, 793, 829
BstYI RGATCY 6 cut(s) 184, 343, 370, 729, 793, 829
BstZ17I GTATAC 1 cut(s) 1396
BsuI GTATCC 1 cut(s) 1871
BsuRI GGCC 3 cut(s) 250, 2310, 2314
BtsCI GGATG 6 cut(s) 42, 161, 853, 897, 1255, 1705
BtsI GCAGTG 2 cut(s) 1101, 2367
BtsIMutI CAGTG 7 cut(s) 235, 603, 1101, 1266, 1647, 1864, 2367
Cac8I GCNNGC 6 cut(s) 456, 1117, 1451, 2312, 2458, 2560
CaiI CAGNNNCTG 1 cut(s) 1100
CciI TCATGA 2 cut(s) 300, 2263
CfoI GCGC 2 cut(s) 358, 1121
Cfr10I RCCGGY 1 cut(s) 2310
Cfr13I GGNCC 1 cut(s) 2308
CseI GACGC 1 cut(s) 635
Csp6I GTAC 6 cut(s) 87, 119, 175, 518, 1172, 2285
CviQI GTAC 6 cut(s) 87, 119, 175, 518, 1172, 2285
DraI TTTAAA 1 cut(s) 2440
EaeI YGGCCR 1 cut(s) 2312
Eam1104I CTCTTC 3 cut(s) 1047, 1425, 1577
EarI CTCTTC 3 cut(s) 1047, 1425, 1577
Ecl136II GAGCTC 1 cut(s) 1593
Eco130I CCWWGG 4 cut(s) 320, 887, 1625, 2484
Eco147I AGGCCT 1 cut(s) 250
Eco24I GRGCYC 1 cut(s) 1595
Eco32I GATATC 1 cut(s) 712
Eco53kI GAGCTC 1 cut(s) 1593
Eco57I CTGAAG 4 cut(s) 561, 1020, 1824, 2406
Eco72I CACGTG 1 cut(s) 9
Eco88I CYCGRG 1 cut(s) 1089
Eco91I GGTNACC 1 cut(s) 1737
EcoICRI GAGCTC 1 cut(s) 1593
EcoO65I GGTNACC 1 cut(s) 1737
EcoRI GAATTC 2 cut(s) 296, 1012
EcoRV GATATC 1 cut(s) 712
EcoT14I CCWWGG 4 cut(s) 320, 887, 1625, 2484
EcoT22I ATGCAT 1 cut(s) 1455
EcoT38I GRGCYC 1 cut(s) 1595
ErhI CCWWGG 4 cut(s) 320, 887, 1625, 2484
FaqI GGGAC 2 cut(s) 1265, 2114
FauI CCCGC 1 cut(s) 1959
FblI GTMKAC 1 cut(s) 1395
FokI GGATG 6 cut(s) 49, 148, 860, 904, 1242, 1712
FriOI GRGCYC 1 cut(s) 1595
FseI GGCCGGCC 1 cut(s) 2314
FspBI CTAG 8 cut(s) 141, 360, 888, 1110, 1626, 1695, 1902, 2508
GlaI GCGC 2 cut(s) 357, 1120
GsuI CTGGAG 4 cut(s) 204, 1389, 1583, 1757
HaeIII GGCC 3 cut(s) 250, 2310, 2314
HapII CCGG 2 cut(s) 2311, 2387
HgaI GACGC 1 cut(s) 635
HhaI GCGC 2 cut(s) 358, 1121
Hin6I GCGC 2 cut(s) 356, 1119
HinP1I GCGC 2 cut(s) 356, 1119
HincII GTYRAC 2 cut(s) 744, 1266
HindII GTYRAC 2 cut(s) 744, 1266
HindIII AAGCTT 1 cut(s) 2402
HpaII CCGG 2 cut(s) 2311, 2387
HphI GGTGA 9 cut(s) 290, 325, 692, 800, 965, 1337, 1731, 2045, 2132
Hpy166II GTNNAC 6 cut(s) 119, 316, 744, 1266, 1396, 1862
Hpy8I GTNNAC 6 cut(s) 119, 316, 744, 1266, 1396, 1862
Hpy99I CGWCG 1 cut(s) 152
HpyAV CCTTC 7 cut(s) 654, 1155, 1222, 1320, 1359, 1546, 2178
HpyCH4IV ACGT 5 cut(s) 8, 672, 688, 1000, 2184
HpyF10VI GCNNNNNNNGC 9 cut(s) 242, 485, 1121, 1130, 1231, 1721, 2143, 2152, 2555
HpySE526I ACGT 5 cut(s) 8, 672, 688, 1000, 2184
HspAI GCGC 2 cut(s) 356, 1119
Kpn2I TCCGGA 1 cut(s) 2386
KroI GCCGGC 1 cut(s) 2310
KroNI GCCGGC 1 cut(s) 2312
LguI GCTCTTC 1 cut(s) 1425
LmnI GCTCC 4 cut(s) 242, 1564, 1776, 1966
Lsp1109I GCAGC 9 cut(s) 337, 633, 1133, 1447, 1711, 1773, 2149, 2167, 2536
LweI GCATC 4 cut(s) 488, 790, 1264, 2245
MaeI CTAG 8 cut(s) 141, 360, 888, 1110, 1626, 1695, 1902, 2508
MaeII ACGT 5 cut(s) 8, 672, 688, 1000, 2184
MaeIII GTNAC 4 cut(s) 503, 1737, 2059, 2339
MbiI CCGCTC 2 cut(s) 1987, 2269
MflI RGATCY 6 cut(s) 184, 343, 370, 729, 793, 829
MhlI GDGCHC 3 cut(s) 460, 1088, 1595
MlyI GAGTC 6 cut(s) 917, 1294, 2093, 2285, 2362, 2552
MmeI TCCRAC 2 cut(s) 220, 655
Mph1103I ATGCAT 1 cut(s) 1455
MroI TCCGGA 1 cut(s) 2386
MroNI GCCGGC 1 cut(s) 2310
MroXI GAANNNNTTC 1 cut(s) 1012
MseI TTAA 3 cut(s) 257, 701, 2439
MspA1I CMGCKG 1 cut(s) 1724
MspI CCGG 2 cut(s) 2311, 2387
Mva1269I GAATGC 1 cut(s) 857
MvnI CGCG 1 cut(s) 646
MwoI GCNNNNNNNGC 9 cut(s) 242, 485, 1121, 1130, 1231, 1721, 2143, 2152, 2555
NaeI GCCGGC 1 cut(s) 2312
NgoMIV GCCGGC 1 cut(s) 2310
NlaIV GGNNCC 8 cut(s) 186, 244, 345, 731, 1320, 1779, 1962, 2047
NmuCI GTSAC 1 cut(s) 1737
NsiI ATGCAT 1 cut(s) 1455
NspI RCATGY 2 cut(s) 214, 1453
PaeI GCATGC 1 cut(s) 1453
PaeR7I CTCGAG 1 cut(s) 1089
PagI TCATGA 2 cut(s) 300, 2263
PasI CCCWGGG 1 cut(s) 1283
PceI AGGCCT 1 cut(s) 250
PciSI GCTCTTC 1 cut(s) 1425
PctI GAATGC 1 cut(s) 857
PdiI GCCGGC 1 cut(s) 2312
PdmI GAANNNNTTC 1 cut(s) 1012
PflFI GACNNNGTC 1 cut(s) 1735
PflMI CCANNNNNTGG 1 cut(s) 2056
PfoI TCCNGGA 3 cut(s) 180, 1773, 1873
PleI GAGTC 6 cut(s) 917, 1294, 2093, 2284, 2362, 2551
PmaCI CACGTG 1 cut(s) 9
PmlI CACGTG 1 cut(s) 9
PpsI GAGTC 6 cut(s) 917, 1294, 2093, 2284, 2362, 2551
Ppu21I YACGTR 2 cut(s) 9, 689
Psp124BI GAGCTC 1 cut(s) 1595
PspCI CACGTG 1 cut(s) 9
PspEI GGTNACC 1 cut(s) 1737
PspN4I GGNNCC 8 cut(s) 186, 244, 345, 731, 1320, 1779, 1962, 2047
PspPI GGNCC 1 cut(s) 2308
PspXI VCTCGAGB 1 cut(s) 1089
PsrI GAACNNNNNNTAC 2 cut(s) 2233, 2265
PstI CTGCAG 2 cut(s) 623, 1729
PstNI CAGNNNCTG 1 cut(s) 1100
PsuI RGATCY 6 cut(s) 184, 343, 370, 729, 793, 829
PsyI GACNNNGTC 1 cut(s) 1735
PvuII CAGCTG 1 cut(s) 1724
RigI GGCCGGCC 1 cut(s) 2314
RsaI GTAC 6 cut(s) 88, 120, 176, 519, 1173, 2286
RsaNI GTAC 6 cut(s) 87, 119, 175, 518, 1172, 2285
SacI GAGCTC 1 cut(s) 1595
SapI GCTCTTC 1 cut(s) 1425
SaqAI TTAA 3 cut(s) 257, 701, 2439
Sau96I GGNCC 1 cut(s) 2308
ScaI AGTACT 1 cut(s) 1173
SchI GAGTC 6 cut(s) 917, 1294, 2093, 2285, 2362, 2552
SduI GDGCHC 3 cut(s) 460, 1088, 1595
SfaNI GCATC 4 cut(s) 488, 790, 1264, 2245
SfcI CTRYAG 2 cut(s) 619, 1725
Sfr274I CTCGAG 1 cut(s) 1089
SlaI CTCGAG 1 cut(s) 1089
SmiI ATTTAAAT 1 cut(s) 2440
SmlI CTYRAG 3 cut(s) 1089, 2064, 2273
SmoI CTYRAG 3 cut(s) 1089, 2064, 2273
SphI GCATGC 1 cut(s) 1453
SseBI AGGCCT 1 cut(s) 250
SsiI CCGC 6 cut(s) 644, 1149, 1952, 1987, 2269, 2357
SspI AATATT 1 cut(s) 2236
SspMI CTAG 8 cut(s) 141, 360, 888, 1110, 1626, 1695, 1902, 2508
SstI GAGCTC 1 cut(s) 1595
StuI AGGCCT 1 cut(s) 250
StyI CCWWGG 4 cut(s) 320, 887, 1625, 2484
SwaI ATTTAAAT 1 cut(s) 2440
TaiI ACGT 5 cut(s) 11, 675, 691, 1003, 2187
TaqI TCGA 5 cut(s) 97, 556, 1090, 1540, 1783
TaqII GACCGA 1 cut(s) 1857
TatI WGTACW 1 cut(s) 1171
TauI GCSGC 3 cut(s) 646, 2272, 2360
Tru1I TTAA 3 cut(s) 257, 701, 2439
Tru9I TTAA 3 cut(s) 257, 701, 2439
TscAI CASTG 7 cut(s) 235, 603, 1101, 1266, 1654, 1864, 2367
TseFI GTSAC 1 cut(s) 1737
TseI GCWGC 9 cut(s) 325, 621, 1121, 1435, 1724, 1761, 2137, 2155, 2549
Tsp45I GTSAC 1 cut(s) 1737
TspGWI ACGGA 2 cut(s) 165, 2111
TspRI CASTG 7 cut(s) 235, 603, 1101, 1266, 1654, 1864, 2367
Tth111I GACNNNGTC 1 cut(s) 1735
Van91I CCANNNNNTGG 1 cut(s) 2056
XapI RAATTY 9 cut(s) 52, 284, 296, 636, 1012, 1536, 1630, 2222, 2435
XbaI TCTAGA 1 cut(s) 1901
XceI RCATGY 2 cut(s) 214, 1453
XcmI CCANNNNNNNNNTGG 1 cut(s) 1280
XhoI CTCGAG 1 cut(s) 1089
XmaJI CCTAGG 2 cut(s) 887, 1625
XmiI GTMKAC 1 cut(s) 1395
XmnI GAANNNNTTC 1 cut(s) 1012
XspI CTAG 8 cut(s) 141, 360, 888, 1110, 1626, 1695, 1902, 2508
ZrmI AGTACT 1 cut(s) 1173
Zsp2I ATGCAT 1 cut(s) 1455
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.