Rorug05G0060600

PB1 domain

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000005
OG0004019
Physical Location & Seq
Forward (+)
5270331 .. 5276257
5927 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug05G0060600.1

Sequence Viewer

Length: 567 bp
ATGATAGGCAGGAAAATCCCAGGCGCCGGAGACAGCAAATCTGCTAAGGCGGAGGAGAGCGATAGCGAGGAGATCGAGGATATTTTTGAAGAACTCAAAATATGGGCATCGAACACGAGGAGTGCAAAGAGAGCAAGGAGAGCAAAAAGAGCAAGAAGAGCACGACGCGGCCGGAGATTTGCTAGCAAGGTCAAGTTCTGGAAAGTAAACGCACCTTCTATAATTCTACCCGTGGCAATAAAACCAGAGACCGTCACTATTCCTGGCAAAAATGGTGCTGATCCTGTTACAATCTCCGTGTTCGATTGTTCAGTCCCATATAAGCCAAAGATCCGCAGAGGCCCCTGGCCCGAAATACCCCTTTCTCATGATGTGAATGGTATTGATTCCGTGTTGAAGTTCATTCTTGGAGGTTGTGATCCAAGTGGTAGCAAGGTCACCGAGGTGAGGAAAGCAGAGCGTTCACCTGCTGGGCCTTCACATGGTAGCAAGGTCATGGCCGGGAAAGCGAAATGTGCACCTGCTGGGCCTTCACTTGATTCGGACAGTCCCCTCCTAAGGGCTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
PB1 Pkinase_Tyr

Protein Analysis

188

Amino Acids

20.29

Weight (kDa)

10.09

Isoelectric Point (pI)

62.56

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 2 cut(s) 475, 529
Acc36I ACCTGC 2 cut(s) 475, 529
AccB1I GGYRCC 1 cut(s) 23
AccII CGCG 1 cut(s) 168
AciI CCGC 3 cut(s) 50, 168, 334
AclWI GGATC 3 cut(s) 275, 325, 413
AcoI YGGCCR 2 cut(s) 169, 498
AcyI GRCGYC 1 cut(s) 24
AfiI CCNNNNNNNGG 5 cut(s) 26, 447, 482, 558, 559
AgsI TTSAA 2 cut(s) 89, 397
AjnI CCWGG 3 cut(s) 19, 262, 344
AleI CACNNNNGTG 1 cut(s) 443
Alw21I GWGCWC 2 cut(s) 163, 520
Alw26I GTCTC 2 cut(s) 24, 242
Alw44I GTGCAC 1 cut(s) 516
AlwI GGATC 3 cut(s) 275, 325, 413
AoxI GGCC 6 cut(s) 169, 340, 347, 473, 498, 527
ApaLI GTGCAC 1 cut(s) 516
AspLEI GCGC 1 cut(s) 26
AspS9I GGNCC 4 cut(s) 341, 348, 473, 527
AsuC2I CCSGG 1 cut(s) 502
AsuHPI GGTGA 3 cut(s) 430, 456, 457
AsuNHI GCTAGC 1 cut(s) 182
AxyI CCTNAGG 1 cut(s) 557
BaeGI GKGCMC 1 cut(s) 520
BanI GGYRCC 1 cut(s) 23
BauI CACGAG 1 cut(s) 115
Bbv12I GWGCWC 2 cut(s) 163, 520
BciT130I CCWGG 3 cut(s) 21, 264, 346
BcnI CCSGG 1 cut(s) 502
BcoDI GTCTC 2 cut(s) 24, 242
BfaI CTAG 1 cut(s) 183
BfoI RGCGCY 1 cut(s) 27
BfuAI ACCTGC 2 cut(s) 475, 529
BisI GCNGC 1 cut(s) 169
BlsI GCNGC 1 cut(s) 170
Bme1390I CCNGG 4 cut(s) 21, 264, 346, 502
BmgT120I GGNCC 4 cut(s) 341, 348, 473, 527
BmiI GGNNCC 2 cut(s) 25, 343
BmrFI CCNGG 4 cut(s) 21, 264, 346, 502
BmsI GCATC 1 cut(s) 116
BmtI GCTAGC 1 cut(s) 186
Bpu10I CCTNAGC 1 cut(s) 45
BpuMI CCSGG 1 cut(s) 502
BsaHI GRCGYC 1 cut(s) 24
BsaI GGTCTC 1 cut(s) 242
BsaJI CCNNGG 4 cut(s) 19, 231, 344, 441
Bsc4I CCNNNNNNNGG 5 cut(s) 26, 447, 482, 558, 559
Bse21I CCTNAGG 1 cut(s) 557
BseBI CCWGG 3 cut(s) 21, 264, 346
BseDI CCNNGG 4 cut(s) 19, 231, 344, 441
BseLI CCNNNNNNNGG 5 cut(s) 26, 447, 482, 558, 559
BseRI GAGGAG 3 cut(s) 68, 83, 133
BseSI GKGCMC 1 cut(s) 520
BseX3I CGGCCG 1 cut(s) 169
BseYI CCCAGC 2 cut(s) 470, 524
Bsh1236I CGCG 1 cut(s) 168
Bsh1285I CGRYCG 1 cut(s) 172
BshFI GGCC 6 cut(s) 171, 342, 349, 475, 500, 529
BshNI GGYRCC 1 cut(s) 23
BsiEI CGRYCG 1 cut(s) 172
BsiHKAI GWGCWC 2 cut(s) 163, 520
BsiSI CCGG 3 cut(s) 27, 172, 501
BslFI GGGAC 2 cut(s) 299, 534
BslI CCNNNNNNNGG 5 cut(s) 26, 447, 482, 558, 559
BsmAI GTCTC 2 cut(s) 24, 242
BsmFI GGGAC 2 cut(s) 299, 534
BsnI GGCC 6 cut(s) 171, 342, 349, 475, 500, 529
Bso31I GGTCTC 1 cut(s) 242
Bsp1286I GDGCHC 2 cut(s) 163, 520
Bsp143I GATC 4 cut(s) 72, 280, 330, 418
BspACI CCGC 3 cut(s) 50, 168, 334
BspANI GGCC 6 cut(s) 171, 342, 349, 475, 500, 529
BspFNI CGCG 1 cut(s) 168
BspHI TCATGA 1 cut(s) 367
BspLI GGNNCC 2 cut(s) 25, 343
BspMI ACCTGC 2 cut(s) 475, 529
BspOI GCTAGC 1 cut(s) 186
BspPI GGATC 3 cut(s) 275, 325, 413
BspQI GCTCTTC 1 cut(s) 151
BspT107I GGYRCC 1 cut(s) 23
BspTNI GGTCTC 1 cut(s) 242
BssECI CCNNGG 4 cut(s) 19, 231, 344, 441
BssMI GATC 4 cut(s) 72, 280, 330, 418
BssNI GRCGYC 1 cut(s) 24
BssSI CACGAG 1 cut(s) 115
Bst2BI CACGAG 1 cut(s) 115
Bst2UI CCWGG 3 cut(s) 21, 264, 346
Bst4CI ACNGT 2 cut(s) 253, 548
Bst6I CTCTTC 1 cut(s) 151
BstACI GRCGYC 1 cut(s) 24
BstC8I GCNNGC 1 cut(s) 184
BstDEI CTNAG 2 cut(s) 45, 557
BstDSI CCRYGG 1 cut(s) 231
BstEII GGTNACC 1 cut(s) 436
BstFNI CGCG 1 cut(s) 168
BstH2I RGCGCY 1 cut(s) 27
BstHHI GCGC 1 cut(s) 26
BstKTI GATC 4 cut(s) 75, 283, 333, 421
BstMAI GTCTC 2 cut(s) 24, 242
BstMBI GATC 4 cut(s) 72, 280, 330, 418
BstMCI CGRYCG 1 cut(s) 172
BstMWI GCNNNNNNNGC 6 cut(s) 131, 140, 149, 158, 506, 515
BstNI CCWGG 3 cut(s) 21, 264, 346
BstPI GGTNACC 1 cut(s) 436
BstSCI CCNGG 4 cut(s) 19, 262, 344, 500
BstSLI GKGCMC 1 cut(s) 520
BstUI CGCG 1 cut(s) 168
BstX2I RGATCY 1 cut(s) 330
BstYI RGATCY 1 cut(s) 330
BstZI CGGCCG 1 cut(s) 169
Bsu36I CCTNAGG 1 cut(s) 557
BsuRI GGCC 6 cut(s) 171, 342, 349, 475, 500, 529
BtgI CCRYGG 1 cut(s) 231
BveI ACCTGC 2 cut(s) 475, 529
Cac8I GCNNGC 1 cut(s) 184
CciI TCATGA 1 cut(s) 367
CfoI GCGC 1 cut(s) 26
Cfr13I GGNCC 4 cut(s) 341, 348, 473, 527
CseI GACGC 1 cut(s) 174
CviAII CATG 3 cut(s) 368, 482, 496
CviJI RGCY 8 cut(s) 171, 325, 342, 349, 475, 500, 529, 563
CviKI_1 RGCY 8 cut(s) 171, 325, 342, 349, 475, 500, 529, 563
DdeI CTNAG 2 cut(s) 45, 557
DinI GGCGCC 1 cut(s) 25
DpnI GATC 4 cut(s) 74, 282, 332, 420
DpnII GATC 4 cut(s) 72, 280, 330, 418
EaeI YGGCCR 2 cut(s) 169, 498
EagI CGGCCG 1 cut(s) 169
Eam1104I CTCTTC 1 cut(s) 151
EarI CTCTTC 1 cut(s) 151
EciI GGCGGA 1 cut(s) 65
EclXI CGGCCG 1 cut(s) 169
Eco31I GGTCTC 1 cut(s) 242
Eco52I CGGCCG 1 cut(s) 169
Eco81I CCTNAGG 1 cut(s) 557
Eco91I GGTNACC 1 cut(s) 436
EcoO109I RGGNCCY 1 cut(s) 341
EcoO65I GGTNACC 1 cut(s) 436
EcoRII CCWGG 3 cut(s) 19, 262, 344
EgeI GGCGCC 1 cut(s) 25
EheI GGCGCC 1 cut(s) 25
FaeI CATG 3 cut(s) 371, 485, 499
FaiI YATR 7 cut(s) 103, 221, 319, 321, 369, 483, 497
FaqI GGGAC 2 cut(s) 299, 534
FatI CATG 3 cut(s) 367, 481, 495
Fnu4HI GCNGC 1 cut(s) 169
Fsp4HI GCNGC 1 cut(s) 169
FspBI CTAG 1 cut(s) 183
GlaI GCGC 1 cut(s) 25
GluI GCNGC 1 cut(s) 169
GsaI CCCAGC 2 cut(s) 474, 528
HaeII RGCGCY 1 cut(s) 27
HaeIII GGCC 6 cut(s) 171, 342, 349, 475, 500, 529
HapII CCGG 3 cut(s) 27, 172, 501
HgaI GACGC 1 cut(s) 174
HhaI GCGC 1 cut(s) 26
Hin1I GRCGYC 1 cut(s) 24
Hin1II CATG 3 cut(s) 371, 485, 499
Hin6I GCGC 1 cut(s) 24
HinP1I GCGC 1 cut(s) 24
HinfI GANTC 2 cut(s) 386, 539
HpaII CCGG 3 cut(s) 27, 172, 501
HphI GGTGA 3 cut(s) 430, 456, 457
Hpy166II GTNNAC 3 cut(s) 208, 464, 518
Hpy188I TCNGA 1 cut(s) 544
Hpy188III TCNNGA 2 cut(s) 199, 368
Hpy8I GTNNAC 3 cut(s) 208, 464, 518
Hpy99I CGWCG 1 cut(s) 168
HpyAV CCTTC 3 cut(s) 225, 486, 540
HpyCH4III ACNGT 2 cut(s) 253, 548
HpyCH4V TGCA 2 cut(s) 125, 518
HpyF10VI GCNNNNNNNGC 6 cut(s) 131, 140, 149, 158, 506, 515
HpyF3I CTNAG 2 cut(s) 45, 557
Hsp92I GRCGYC 1 cut(s) 24
Hsp92II CATG 3 cut(s) 371, 485, 499
HspAI GCGC 1 cut(s) 24
KasI GGCGCC 1 cut(s) 23
Kzo9I GATC 4 cut(s) 72, 280, 330, 418
LguI GCTCTTC 1 cut(s) 151
LweI GCATC 1 cut(s) 116
MaeI CTAG 1 cut(s) 183
MaeIII GTNAC 3 cut(s) 253, 286, 436
MalI GATC 4 cut(s) 74, 282, 332, 420
MboI GATC 4 cut(s) 72, 280, 330, 418
MboII GAAGA 2 cut(s) 101, 168
MflI RGATCY 1 cut(s) 330
MhlI GDGCHC 2 cut(s) 163, 520
MluCI AATT 1 cut(s) 222
Mly113I GGCGCC 1 cut(s) 24
MnlI CCTC 9 cut(s) 46, 61, 70, 111, 332, 404, 436, 441, 563
MseI TTAA 1 cut(s) 565
MslI CAYNNNNRTG 1 cut(s) 443
MspI CCGG 3 cut(s) 27, 172, 501
MspR9I CCNGG 4 cut(s) 21, 264, 346, 502
MvaI CCWGG 3 cut(s) 21, 264, 346
MvnI CGCG 1 cut(s) 168
MwoI GCNNNNNNNGC 6 cut(s) 131, 140, 149, 158, 506, 515
NarI GGCGCC 1 cut(s) 24
NciI CCSGG 1 cut(s) 502
NdeII GATC 4 cut(s) 72, 280, 330, 418
NheI GCTAGC 1 cut(s) 182
NlaIII CATG 3 cut(s) 371, 485, 499
NlaIV GGNNCC 2 cut(s) 25, 343
NmuCI GTSAC 2 cut(s) 253, 436
OliI CACNNNNGTG 1 cut(s) 443
PagI TCATGA 1 cut(s) 367
PaqCI CACCTGC 2 cut(s) 475, 529
PciSI GCTCTTC 1 cut(s) 151
PfeI GAWTC 2 cut(s) 386, 539
PkrI GCNGC 1 cut(s) 170
PluTI GGCGCC 1 cut(s) 27
Psp6I CCWGG 3 cut(s) 19, 262, 344
PspEI GGTNACC 1 cut(s) 436
PspFI CCCAGC 2 cut(s) 470, 524
PspGI CCWGG 3 cut(s) 19, 262, 344
PspN4I GGNNCC 2 cut(s) 25, 343
PspPI GGNCC 4 cut(s) 341, 348, 473, 527
PsuI RGATCY 1 cut(s) 330
RseI CAYNNNNRTG 1 cut(s) 443
SapI GCTCTTC 1 cut(s) 151
SaqAI TTAA 1 cut(s) 565
SatI GCNGC 1 cut(s) 169
Sau3AI GATC 4 cut(s) 72, 280, 330, 418
Sau96I GGNCC 4 cut(s) 341, 348, 473, 527
ScrFI CCNGG 4 cut(s) 21, 264, 346, 502
SduI GDGCHC 2 cut(s) 163, 520
SetI ASST 8 cut(s) 192, 217, 415, 438, 447, 469, 495, 523
SfaNI GCATC 1 cut(s) 116
SfoI GGCGCC 1 cut(s) 25
SmiMI CAYNNNNRTG 1 cut(s) 443
Sse9I AATT 1 cut(s) 222
SsiI CCGC 3 cut(s) 50, 168, 334
SspDI GGCGCC 1 cut(s) 23
SspMI CTAG 1 cut(s) 183
StyD4I CCNGG 4 cut(s) 19, 262, 344, 500
TaaI ACNGT 2 cut(s) 253, 548
TaqI TCGA 3 cut(s) 75, 110, 303
TasI AATT 1 cut(s) 222
TauI GCSGC 1 cut(s) 171
TfiI GAWTC 2 cut(s) 386, 539
Tru1I TTAA 1 cut(s) 565
Tru9I TTAA 1 cut(s) 565
TseFI GTSAC 2 cut(s) 253, 436
Tsp45I GTSAC 2 cut(s) 253, 436
TspDTI ATGAA 1 cut(s) 391
TspGWI ACGGA 2 cut(s) 286, 379
VneI GTGCAC 1 cut(s) 516
XspI CTAG 1 cut(s) 183
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.