RLG00000032538

PB1 domain

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr7
OG0004019
Physical Location & Seq
Reverse (-)
16237114 .. 16250745
13632 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000032538

Sequence Viewer

Length: 6144 bp
ATGATCATCTCCAAAGCTTCAAACTTTACTGCCCACGGAGCTCTGTCCCCTCTCTACACCCCCAAATGGGCCCTCAAAAGCCCACACCCTCACGTCTGCATGGTCTTCTCGTCCTCCGCCGACGGACCCAGATGGAACCAGTGCCACCACTTCAACGGCAACAAAAACGGCGCCGTTTACAAGAAACCCAGAAACGCAGAAGAGGCAGATGAAGAAGGAGGAGAGAGAAGGGTGAGCTGTGAAGTGGATATGATTTCGTGGCGGGAGCGCCGAGTCAAGGCCGAGATTTCGGTCAATGCCGACGTCGATTCCGTCTGGAACGCTCTCACGGATTACGAGCGTCTCGCTGACTTCATCCCCAATCTTGTTTCCAGTGGGAGGATTCCATGTCCACATCCTGGGCGTATTTGGCTGGAGCAGAGAGGCTTGCAGAGAGCACTGTATTGGCACATCGAAGCTCGAGTTGTTTTGGACCTTCAGGAGTTTCCCAATTTGAGTGGTAATGATCGTGAGCTTCACTTTTCCATGGTTGATGGGGACTTCAAAAAGTTTGAAGGCAAATGGTCTGTGAAATGCGGGACAAGGTCATCATCAACAATTTTATCATATGAAGTAAATGTAATACCGAGATTCAACTTCCCTGCCATTTTCTTGGAGAGGATTATCAGATCAGATCTCCCTGTCAATCTTCGGGCCTTGGCTTGTAGGTCTGAAAAGGCTTTTGAAGGGGGAAAAAAGGTAGCAATAGAAGAAAGCTCCAAGCCTAGCATGACTAATGGTGTCACTAGTTCACTCCAAAAAGACATGGATGGTTCTTTGTGTGAAAAGGATTATACACTTAACGAGTTTAAAGAGAATTTACCTGGCCCTAGTGCAAAATCTTTGCCCCCAGCTTCTTCTGAGTTGAATAGTAATTGGGGTGTTTTTGGAAAAGTCTGCAGACTTGATAGGCCTTGCTTGGTGGATGAGGTTCATCTTCGGAGATTCGATGGTCTATTGGAAAATGGAGGCGTCCATCGTGGTGTTGTTGCTAGCATAACGGTAAAAGCTCCTGTTCGTGAAGTATGGGGTGTTTTGACTGCTTACGAAAGTCTTCCCGAGATAGTCCCGAATTTGGCAATCAGTCGGATATTGTCAAGAGAAAACAACAAAGTCCGAATTCTTCAGGAAGGATGTAAGGGCTTACTTTATATGGTACTTCATGCACGTGTTGTCCTAGACTTGTGCGAACAGCTTGAACAGGAGATAAGTTTTGAACAAGTTGAAGGGGACTTCGACTCATTTAGGGGTAAATGGGTTCTTGAGCAGCTAGGAAGTCATCACACGCTGTTGAAGTACACTGTTGAATCGAAAATGCGCAAGGATACTTTTCTCTCTGAAGCTATCATGGAAGAGGTTATATACGAAGATCTCCCTTCAAATTTATGTACAATACGAGACTACATAGAAAAGAGGGGGGTAGCACAGTCATCGAAAGCATGTGATGAAAAAATAAGCTTGGAGGAACAAACTGCTTCATCCAGTACAGACCATAATGAGGATGGTTGTATAACAGTTGATCAATTGTCTGATTCTGATGATCAGAGTTCATCTAGTCAAAGGCCAAGGGTCCCAGGATTGCAGAGGGATATTGAAGTTTTGAAAGCCGAGCTCCTGAAATTTATTGCAGAGCATGGGCAGGAAGGATTTATGCCCATGAGAAAGCAACTTCGTTTACGGGGAAGAGTAGATATCGAGAAAGCAATCACACGCATGGGTGGATTCAGAAGGATTGCAACACTGATGAATCTTTCTCTTGCTTATAAGCATCGAAAACCAAAGGGTTACTGGGATAATCTTGAGAATTTGCAGGAAGAGATTAGTCGATTTCAGAGGAGCTGGGGAATGGACCCTTCATACATGCCCAGCAGGAAGTCATTCGAGCGTGCAGGGCGGTATGACATTGCGCGTGCATTAGAAAAGTGGGGCGGCCTTCACGAAGTTTCGCGTCTTCTGTCACTCAATGTGAGGCACCCCAATAGACGGCTGAACATTGCCAGGGATGTAAAAAGTGATCGTGTAGTATCAACTAACGCAGAGGGTGCAAAAGATGCACCATCCAATCCCTACAGAAAAGTTGAGCACACAAAAACACAGAGACTTGCAGATGATCCACCACAGTTGATTTCAAGGCTCCTCTTCAAAGACTGCTGGTTTTACCTTATGGATAGAAATCTGGGTCAAGGTGCAATGGATCAACCAAAAAACTACGATCAAGTTCGATACAGCACTGTTGAAACTAGAAACGACGGATATGGGTCTGCCAATCAACGGTTTTTCCCCGATCCATCAAGTAATATTAGCACTAATATGCGACCTCCAGAGTATAATGTTTCTGGTGGAACCAGACCTGTATTAAATTACTCTATTCAGACTGGCGAGGAATTTGCTCTTGAATTTATGCGGGAAAGGGTGAACTCGAGGCAGCATTTATTAGTCCCACATGCATCTGGGGATCCTAATAGTGCTTCTAGATATATGGGTCTAAAAGGCATACTTGGAATCAGCCATACAGGCTCAGAGAGTGGGTCCGATGTTTCTATGCTGAATTTGGTAGAGAAAGATCATGTCCCGGAAAATGAAACAAAGGCATCTTCTCCTCTTGAAAATAAAAGTTACTATGATTCAGTACGGTTACCACAACCCTCATCAAGAAATGACATCAATCGAGGACTTTCTGGTTATTCCTCTTCAGGAGTGTCTGATAGCTCATCGAAAAAAGTGAAGTTCCTTTGCAGCTTTGGTGGTAAAATCTTGCCTCGTCCTAGTGACGGAAAGCTTAGATACGTTGGAGGTGAAACACGTATTATCCGGATAACCAAGGATATCGTTTGGCAGGATCTTATGCAGAAACTGTTGGCAGTTTATGACCAAACTCATACAATAAAGTATCAGCTTCCGGGCGAGGATCTTGATGCATTAGTTTCGGTTTCTTCTGATGAGGATCTGCAAAATATGATGGAGGAATGCACTATTCTACAAGATGGAGGATCACAGAGGCCTAGGATGTTCCTGTTTTCTAGTCTTGATTTAGAGGAATCTCAATTTGGCCCTGAAAGCGTGGAGGGTGATTCTGAGATTCAATATGTTGTTGCTGTAAACGGGATGGACCTAGGTTCAAAAAAGAATTCTATTGCTTTGGCAAGTTCTTCAGGCAACAATTTGGAAGAGTTACTCAGTCTAAATGTTACAAGGGCCAGCACTCACACAGTGCCTGATGCAGCTCCTGCTGGCACTGTTCCTTCAGCGGTAGATTTGCCTTCCTCAGTTAATCAAGCTTCTCATTCAGCGGTTCCTGGTTCTTCAAGTGCTTATGAATCCAACTCACAGCTTTATCAGGGACAAAAAATGCACAGTGGAGACACCCGACAGTATCCATCATCAGCTTTGAACCCTGTGGAGAGCTTCCCTGCAAAAGATGAACAGACTACAGTGCTCTCATCTGTTCCAGTGCAATATGATTTCGGTTCTCAACCTCCTAATTACGCAATTGGAGAAAATATAGGTTCTATGCCTATCTATGGACAGCCGATTCAACAAGGGGGTCTGATTGAAGATCAGCTATATGCTGGAATACATGGGCAAGATTCAGAATTACCCATAAAGGAGATGAAACTGAAAAGAGATAGCTCGGCCCAAAAGATAAATGAAGCTGAGAAAGTTCAATCTTTAGAGAAGGATACTCCACAGAAGGAAGCAAGAATGACAAGAGAGAGCTCTCTCCAGAAGCTAAATGAAACTGATAAAGTTCGAAGTTTAGAAAATGAGAAGACTGTTTCTGTAAATCCATATGATGGTTCTGTTCCAAATTATATTGCTAGGGATGAAGTTTCAGTTGCCAATTCAGTAGCAGAGACAGGTTCACCCTTATTGGCTACAAGAAGCAACAAAAAGCTCCAGGAACCTCGACAGAATCCAACAACCTCTGAAGATGTAAATGATGGGAAAAAGAACAATGAAGATGATCGTTTCCACACAGCAACCAGCGGACTGTCTATTCCTGGATATGCTGGCTCAGAAGTTGACTCTAGATATGCTGGCTCTGAGGTTGATTCTATGGATTTTAGCTATCTTGAACAACCTGTGGTTCCTCCGCGTGTCTATCATTCAGAACGAATCCCCAGGGAGCAATCAGAACTGAACCGTCTATCAAAATCTGGGGACTCTTTTGGTTCGCCGTTTATGGTAGCTCAGGCACGTCCTGATCACAAACAACCAATCATGGAATCTGTTGAGAAATTGCATGATGAGAATGTCACTTTGCAGTCTGAGCAGTCTGTCTTACCTTCAAAACTACTGTACAAAATTCCTCAGACAGTTGAAGAGGGGCTTGAGCGAAAGGTACAAAAACCTGACTCAATACATCCAGTTGGTAATTCTGGTGATGGCCGTGAAACGGGCAGGCTAAAGGATAACTACGGAGACCGAAGTATCAGTGATAAACAAGCAGCGCTGACTCAACTAAGAGCTGGTCAAGAAACGTCTCTCAAGCCTACAGATGATTCTGCTTCAGTTCCACCAGAATTTGAATGGACCGAGATAACTGCCAGTAAGGACTACGGAAATAATGGCAAGGTTTATGTAAATCTTGTGGCTCGGAAGGAGAACCCAATTACAGGTGGTGGTAATGGCGAGTCTGCTGTTGGTGTTGCCACTACAGAGCACGGGGATATTCTTATTGATATTAATGATCGTTTTCCCCGTGATTTTCTGTCTGATATATTTTCCAAAGCTAGGATAGACCAGTCTGGTGTCAGTCCACTTCCTGGGGATGGAACTGGCTTGAGCTTGAACATGGAGAATCATGAACCTATGCATTGGTCATACTTCCGAAATTTGGCTCAGAATGAATTTGTAAGAAAAGATGTCTCCCTTATGGATCAGGACCATCTGGGTTTCTCATCCCCACTGACAGGTATTGGAGAAGGAGCTCCAGTTGATTACAGCTATCCACCTTTGAAGTCTGCTGGAGTTGTTTTTGGTCACACTGAATCACATATCAGTTTTGATGAGGATATTCGGCAAGATTTGTCATCTATAACTGGACCCACCACCATAAACGTAAATTCAGATTATAATCCTTCTCTACCTGAGGGAATTGAAAGTGAGCAGGTGGATGGGGTAAATCATAGAGTACGAGAATCAGAATATGAGGATGACAAGCTAGAGAACAAGAACATAGCTGGACCACCTGGTGATCTTCCTCTGGAAGATTTTGATATTACTACCTTGCAGATCATAAAAAATGAAGATCTTGAAGAGCTTAGGGAATTGGGTTCTGGCACGTTTGGGACTGTTTATCATGGAAAGTGGAGGGGAACAGATGTTGCCATTAAGCGAATAAAAAAGAGCTGTTTCACAGGGCGCTCATCAGAGCAAGAAAGATTGACGGTTGAGTTCTGGCGTGAAGCTGAAATTCTTTCAAAGCTTCACCATCCAAATGTGGTGGCATTTTATGGGGTCGTGCAGGACGGACCAGGGGCAACGCTAGCCACTGTGACCGAGTTCATGGTGAATGGCTCTCTGAGGCATGTTTTACTTAGCAAGGAGAGGCATCTTGATCGTCGTAAAAGGCTCATTATTGCTATGGATGCAGCATTTGGAATGGAGTATCTGCATTCCAAAAATATCGTGCACTTTGATTTGAAATGTGACAATTTGCTTGTGAACTTGAAAGACCCTTTACGACCCATTTGCAAGGTAGGAGATTTTGGGCTGTCTAAAATCAAAAGAAATACCTTAGTTACGGGTGGTGTGAGGGGAACCCTTCCATGGATGGCTCCTGAGCTTCTTAATGGTAGCAGTAGTAAGGTTTCAGAAAAGGTTGATGTCTTTTCCTTTGGCATCGTCTTATGGGAGATTCTTACTTGTGAGGAGCCTTATGCCAATATGCATTACGGAGCAATCATAGGAGGCATTGTCAATAACACATTGAGGCCATCTGTACCCAACTATTGTGATGCTGAATGGAAGTTGCTGATGGAACAATGTTGGGCCCCTGATCCCACTGTCCGCCCATCCTTCACGGAAATTGCCAGACGGTTGCGTGTGATGTCTGCAGCTTGCCAAACTAAGCCACAACAGCAGAACCAGGTAGCCAAGTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
PB1 Pkinase_Tyr

Protein Analysis

2048

Amino Acids

228.31

Weight (kDa)

5.63

Isoelectric Point (pI)

48.75

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Polyketide_cyc PF03364 98 - 233 1.1e-16 Polyketide cyclase / dehydrase and lipid transport
Polyketide_cyc PF03364 348 - 475 1.3e-17 Polyketide cyclase / dehydrase and lipid transport
PB1 PF00564 937 - 1020 5.1e-19 PB1 domain
Pkinase PF00069 1766 - 2025 4.6e-47 Protein kinase domain
PK_Tyr_Ser-Thr PF07714 1767 - 2028 1.9e-62 Protein tyrosine and serine/threonine kinase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 2 cut(s) 1803, 5115
AarI CACCTGC 1 cut(s) 5140
AatII GACGTC 1 cut(s) 306
Acc16I TGCGCA 1 cut(s) 1358
Acc36I ACCTGC 1 cut(s) 5140
AccB1I GGYRCC 2 cut(s) 170, 2010
AccB7I CCANNNNNTGG 3 cut(s) 398, 3833, 4805
AccII CGCG 3 cut(s) 1948, 1987, 4135
AccIII TCCGGA 1 cut(s) 2850
AcoI YGGCCR 1 cut(s) 4426
AcuI CTGAAG 8 cut(s) 461, 1148, 1398, 2715, 3174, 3267, 3987, 4533
AcvI CACGTG 1 cut(s) 1208
AcyI GRCGYC 3 cut(s) 171, 303, 1011
AdeI CACNNNGTG 2 cut(s) 3250, 5234
AfaI GTAC 9 cut(s) 1197, 1337, 1429, 1525, 2670, 4340, 4383, 5175, 5985
AfeI AGCGCT 1 cut(s) 4491
AflIII ACRYGT 2 cut(s) 1207, 2840
AhlI ACTAGT 1 cut(s) 785
AjiI CACGTC 2 cut(s) 94, 4238
AjuI GAANNNNNNNTTGG 2 cut(s) 3069, 3101
Alw21I GWGCWC 9 cut(s) 43, 439, 1653, 2124, 3477, 3758, 4704, 4972, 5676
Alw26I GTCTC 8 cut(s) 347, 1431, 2131, 3393, 3887, 4455, 4527, 4912
Alw44I GTGCAC 1 cut(s) 5672
AlwNI CAGNNNCTG 3 cut(s) 2893, 3254, 3266
Ama87I CYCGRG 3 cut(s) 459, 1097, 2458
Aor13HI TCCGGA 1 cut(s) 2850
Aor51HI AGCGCT 1 cut(s) 4491
ApaI GGGCCC 2 cut(s) 73, 6037
ApaLI GTGCAC 1 cut(s) 5672
ApeKI GCWGC 7 cut(s) 1306, 2464, 2775, 3260, 4487, 5633, 6098
AseI ATTAAT 1 cut(s) 4725
Asp700I GAANNNNTTC 2 cut(s) 1092, 1916
AspA2I CCTAGG 2 cut(s) 3041, 3151
AspLEI GCGC 6 cut(s) 173, 270, 1359, 1948, 4492, 5406
AsuC2I CCSGG 2 cut(s) 2612, 2940
AsuHPI GGTGA 9 cut(s) 244, 2464, 2846, 3119, 3894, 4433, 5246, 5462, 5563
AsuII TTCGAA 1 cut(s) 3790
AsuNHI GCTAGC 2 cut(s) 1031, 5527
AvaI CYCGRG 3 cut(s) 459, 1097, 2458
AvrII CCTAGG 2 cut(s) 3041, 3151
AxyI CCTNAGG 1 cut(s) 5130
BaeGI GKGCMC 3 cut(s) 73, 5676, 6037
BaeI ACNNNNGTAYC 2 cut(s) 4453, 4486
BamHI GGATCC 1 cut(s) 2494
BanI GGYRCC 2 cut(s) 170, 2010
BanII GRGCYC 6 cut(s) 43, 73, 1653, 3758, 4972, 6037
BbrPI CACGTG 1 cut(s) 1208
BbsI GAAGAC 4 cut(s) 97, 1085, 1982, 3815
Bbv12I GWGCWC 9 cut(s) 43, 439, 1653, 2124, 3477, 3758, 4704, 4972, 5676
BbvI GCAGC 7 cut(s) 1318, 2476, 2787, 3272, 4499, 5645, 6110
BceAI ACGGC 6 cut(s) 158, 172, 184, 2039, 4201, 4413
BciVI GTATCC 3 cut(s) 1357, 3423, 3712
BclI TGATCA 4 cut(s) 3, 1558, 1579, 4243
BcnI CCSGG 2 cut(s) 2612, 2940
BcoDI GTCTC 8 cut(s) 347, 1431, 2131, 3393, 3887, 4455, 4527, 4912
BcuI ACTAGT 1 cut(s) 785
BfmI CTRYAG 6 cut(s) 937, 2107, 3468, 4533, 4695, 6096
BfoI RGCGCY 4 cut(s) 174, 271, 4493, 5407
BfuAI ACCTGC 1 cut(s) 5140
BfuI GTATCC 3 cut(s) 1357, 3423, 3712
BglI GCCNNNNNGGC 1 cut(s) 2553
BglII AGATCT 3 cut(s) 673, 1408, 5290
BisI GCNGC 8 cut(s) 1307, 1969, 2465, 2776, 3261, 4488, 5634, 6099
BlnI CCTAGG 2 cut(s) 3041, 3151
BlsI GCNGC 8 cut(s) 1308, 1970, 2466, 2777, 3262, 4489, 5635, 6100
BmeT110I CYCGRG 3 cut(s) 459, 1097, 2458
BmgBI CACGTC 2 cut(s) 94, 4238
BmrI ACTGGG 1 cut(s) 1837
BmtI GCTAGC 2 cut(s) 1035, 5531
BmuI ACTGGG 1 cut(s) 1837
BpiI GAAGAC 4 cut(s) 97, 1085, 1982, 3815
BpmI CTGGAG 6 cut(s) 434, 2343, 3746, 3920, 4956, 5028
Bpu10I CCTNAGC 3 cut(s) 4230, 5303, 5823
Bpu14I TTCGAA 1 cut(s) 3790
BpuEI CTTGAG 5 cut(s) 1322, 1859, 4391, 4511, 4843
BpuMI CCSGG 2 cut(s) 2612, 2940
BsaAI YACGTR 2 cut(s) 1208, 2843
BsaBI GATNNNNATC 3 cut(s) 2211, 2982, 5115
BsaHI GRCGYC 3 cut(s) 171, 303, 1011
BsaI GGTCTC 1 cut(s) 4455
BsaWI WCCGGW 1 cut(s) 2850
BsaXI ACNNNNNCTCC 4 cut(s) 4959, 4989, 5943, 5973
Bse21I CCTNAGG 1 cut(s) 5130
Bse3DI GCAATG 3 cut(s) 1941, 2031, 2235
Bse8I GATNNNNATC 3 cut(s) 2211, 2982, 5115
BseAI TCCGGA 1 cut(s) 2850
BseJI GATNNNNATC 3 cut(s) 2211, 2982, 5115
BseMI GCAATG 3 cut(s) 1941, 2031, 2235
BseRI GAGGAG 5 cut(s) 234, 1888, 2165, 2628, 5927
BseSI GKGCMC 3 cut(s) 73, 5676, 6037
BseXI GCAGC 7 cut(s) 1318, 2476, 2787, 3272, 4499, 5645, 6110
BseYI CCCAGC 3 cut(s) 889, 1878, 1904
BsgI GTGCAG 2 cut(s) 1947, 5525
Bsh1236I CGCG 3 cut(s) 1948, 1987, 4135
BshNI GGYRCC 2 cut(s) 170, 2010
BsiHKAI GWGCWC 9 cut(s) 43, 439, 1653, 2124, 3477, 3758, 4704, 4972, 5676
BsiHKCI CYCGRG 3 cut(s) 459, 1097, 2458
BsiSI CCGG 3 cut(s) 2612, 2851, 2939
BsmAI GTCTC 8 cut(s) 347, 1431, 2131, 3393, 3887, 4455, 4527, 4912
BsmBI CGTCTC 2 cut(s) 347, 4527
BsmI GAATGC 2 cut(s) 3011, 5656
Bso31I GGTCTC 1 cut(s) 4455
BsoBI CYCGRG 3 cut(s) 459, 1097, 2458
Bsp119I TTCGAA 1 cut(s) 3790
Bsp120I GGGCCC 2 cut(s) 69, 6033
Bsp13I TCCGGA 1 cut(s) 2850
Bsp1407I TGTACA 2 cut(s) 1427, 4338
Bsp19I CCATGG 2 cut(s) 525, 5810
BspEI TCCGGA 1 cut(s) 2850
BspFNI CGCG 3 cut(s) 1948, 1987, 4135
BspHI TCATGA 1 cut(s) 4843
BspMAI CTGCAG 2 cut(s) 941, 6100
BspMI ACCTGC 1 cut(s) 5140
BspOI GCTAGC 2 cut(s) 1035, 5531
BspQI GCTCTTC 1 cut(s) 5292
BspT104I TTCGAA 1 cut(s) 3790
BspT107I GGYRCC 2 cut(s) 170, 2010
BspTNI GGTCTC 1 cut(s) 4455
BsrDI GCAATG 3 cut(s) 1941, 2031, 2235
BsrGI TGTACA 2 cut(s) 1427, 4338
BssNI GRCGYC 3 cut(s) 171, 303, 1011
BssT1I CCWWGG 7 cut(s) 525, 696, 1604, 2859, 3041, 3151, 5810
Bst6I CTCTTC 9 cut(s) 195, 1386, 1717, 1848, 2183, 2734, 3201, 4356, 5292
BstACI GRCGYC 3 cut(s) 171, 303, 1011
BstAPI GCANNNNNTGC 3 cut(s) 2081, 2090, 3266
BstAUI TGTACA 2 cut(s) 1427, 4338
BstBAI YACGTR 2 cut(s) 1208, 2843
BstBI TTCGAA 1 cut(s) 3790
BstDSI CCRYGG 3 cut(s) 34, 525, 5810
BstEII GGTNACC 1 cut(s) 2673
BstFNI CGCG 3 cut(s) 1948, 1987, 4135
BstH2I RGCGCY 4 cut(s) 174, 271, 4493, 5407
BstHHI GCGC 6 cut(s) 173, 270, 1359, 1948, 4492, 5406
BstMAI GTCTC 8 cut(s) 347, 1431, 2131, 3393, 3887, 4455, 4527, 4912
BstNSI RCATGY 4 cut(s) 1482, 1903, 2486, 5573
BstPI GGTNACC 1 cut(s) 2673
BstSFI CTRYAG 6 cut(s) 937, 2107, 3468, 4533, 4695, 6096
BstSLI GKGCMC 3 cut(s) 73, 5676, 6037
BstUI CGCG 3 cut(s) 1948, 1987, 4135
BstV1I GCAGC 7 cut(s) 1318, 2476, 2787, 3272, 4499, 5645, 6110
BstV2I GAAGAC 4 cut(s) 97, 1085, 1982, 3815
BstX2I RGATCY 7 cut(s) 673, 1408, 2494, 2878, 2947, 2983, 5290
BstXI CCANNNNNNTGG 1 cut(s) 652
BstYI RGATCY 7 cut(s) 673, 1408, 2494, 2878, 2947, 2983, 5290
Bsu36I CCTNAGG 1 cut(s) 5130
BsuI GTATCC 3 cut(s) 1357, 3423, 3712
BtgI CCRYGG 3 cut(s) 34, 525, 5810
BtrI CACGTC 2 cut(s) 94, 4238
BveI ACCTGC 1 cut(s) 5140
CaiI CAGNNNCTG 3 cut(s) 2893, 3254, 3266
CciI TCATGA 1 cut(s) 4843
CfoI GCGC 6 cut(s) 173, 270, 1359, 1948, 4492, 5406
CseI GACGC 3 cut(s) 329, 1000, 1976
CsiI ACCWGGT 2 cut(s) 5230, 6129
Csp6I GTAC 9 cut(s) 1196, 1336, 1428, 1524, 2669, 4339, 4382, 5174, 5984
CspCI CAANNNNNGTGG 6 cut(s) 478, 513, 2142, 2177, 5466, 5501
CviQI GTAC 9 cut(s) 1196, 1336, 1428, 1524, 2669, 4339, 4382, 5174, 5984
DinI GGCGCC 1 cut(s) 172
DraI TTTAAA 1 cut(s) 850
DraIII CACNNNGTG 2 cut(s) 3250, 5234
EaeI YGGCCR 1 cut(s) 4426
Eam1104I CTCTTC 9 cut(s) 195, 1386, 1717, 1848, 2183, 2734, 3201, 4356, 5292
EarI CTCTTC 9 cut(s) 195, 1386, 1717, 1848, 2183, 2734, 3201, 4356, 5292
EciI GGCGGA 2 cut(s) 106, 6041
Ecl136II GAGCTC 4 cut(s) 41, 1651, 3756, 4970
Eco130I CCWWGG 7 cut(s) 525, 696, 1604, 2859, 3041, 3151, 5810
Eco147I AGGCCT 2 cut(s) 952, 3040
Eco24I GRGCYC 6 cut(s) 43, 73, 1653, 3758, 4972, 6037
Eco31I GGTCTC 1 cut(s) 4455
Eco32I GATATC 2 cut(s) 1732, 2866
Eco47III AGCGCT 1 cut(s) 4491
Eco53kI GAGCTC 4 cut(s) 41, 1651, 3756, 4970
Eco57I CTGAAG 8 cut(s) 461, 1148, 1398, 2715, 3174, 3267, 3987, 4533
Eco72I CACGTG 1 cut(s) 1208
Eco81I CCTNAGG 1 cut(s) 5130
Eco88I CYCGRG 3 cut(s) 459, 1097, 2458
Eco91I GGTNACC 1 cut(s) 2673
EcoICRI GAGCTC 4 cut(s) 41, 1651, 3756, 4970
EcoO109I RGGNCCY 3 cut(s) 70, 1609, 6034
EcoO65I GGTNACC 1 cut(s) 2673
EcoRI GAATTC 2 cut(s) 1158, 3166
EcoRV GATATC 2 cut(s) 1732, 2866
EcoT14I CCWWGG 7 cut(s) 525, 696, 1604, 2859, 3041, 3151, 5810
EcoT22I ATGCAT 4 cut(s) 2488, 2959, 4857, 5934
EcoT38I GRGCYC 6 cut(s) 43, 73, 1653, 3758, 4972, 6037
EgeI GGCGCC 1 cut(s) 172
EheI GGCGCC 1 cut(s) 172
ErhI CCWWGG 7 cut(s) 525, 696, 1604, 2859, 3041, 3151, 5810
Esp3I CGTCTC 2 cut(s) 347, 4527
FalI AAGNNNNNCTT 4 cut(s) 2520, 2552, 4353, 4385
FauI CCCGC 3 cut(s) 255, 569, 2436
FauNDI CATATG 2 cut(s) 607, 3829
FbaI TGATCA 4 cut(s) 3, 1558, 1579, 4243
Fnu4HI GCNGC 8 cut(s) 1307, 1969, 2465, 2776, 3261, 4488, 5634, 6099
FriOI GRGCYC 6 cut(s) 43, 73, 1653, 3758, 4972, 6037
Fsp4HI GCNGC 8 cut(s) 1307, 1969, 2465, 2776, 3261, 4488, 5634, 6099
FspI TGCGCA 1 cut(s) 1358
GlaI GCGC 6 cut(s) 172, 269, 1358, 1947, 4491, 5405
GluI GCNGC 8 cut(s) 1307, 1969, 2465, 2776, 3261, 4488, 5634, 6099
GsaI CCCAGC 3 cut(s) 893, 1882, 1908
GsuI CTGGAG 6 cut(s) 434, 2343, 3746, 3920, 4956, 5028
HaeII RGCGCY 4 cut(s) 174, 271, 4493, 5407
HapII CCGG 3 cut(s) 2612, 2851, 2939
HgaI GACGC 3 cut(s) 329, 1000, 1976
HhaI GCGC 6 cut(s) 173, 270, 1359, 1948, 4492, 5406
Hin1I GRCGYC 3 cut(s) 171, 303, 1011
Hin6I GCGC 6 cut(s) 171, 268, 1357, 1946, 4490, 5404
HinP1I GCGC 6 cut(s) 171, 268, 1357, 1946, 4490, 5404
HincII GTYRAC 1 cut(s) 4063
HindII GTYRAC 1 cut(s) 4063
HindIII AAGCTT 5 cut(s) 15, 1495, 2816, 3315, 5465
HpaII CCGG 3 cut(s) 2612, 2851, 2939
HphI GGTGA 9 cut(s) 244, 2464, 2846, 3119, 3894, 4433, 5246, 5462, 5563
Hpy99I CGWCG 5 cut(s) 125, 305, 308, 2291, 5607
HpyCH4IV ACGT 9 cut(s) 93, 303, 1207, 2826, 2842, 4237, 4520, 5100, 5324
HpySE526I ACGT 9 cut(s) 93, 303, 1207, 2826, 2842, 4237, 4520, 5100, 5324
Hsp92I GRCGYC 3 cut(s) 171, 303, 1011
HspAI GCGC 6 cut(s) 171, 268, 1357, 1946, 4490, 5404
KasI GGCGCC 1 cut(s) 170
KflI GGGWCCC 1 cut(s) 1609
Kpn2I TCCGGA 1 cut(s) 2850
Ksp22I TGATCA 4 cut(s) 3, 1558, 1579, 4243
LguI GCTCTTC 1 cut(s) 5292
Lsp1109I GCAGC 7 cut(s) 1318, 2476, 2787, 3272, 4499, 5645, 6110
MabI ACCWGGT 2 cut(s) 5230, 6129
MaeII ACGT 9 cut(s) 93, 303, 1207, 2826, 2842, 4237, 4520, 5100, 5324
MfeI CAATTG 2 cut(s) 1562, 3528
MflI RGATCY 7 cut(s) 673, 1408, 2494, 2878, 2947, 2983, 5290
Mly113I GGCGCC 1 cut(s) 171
MlyI GAGTC 7 cut(s) 282, 1271, 4058, 4196, 4388, 4489, 4682
MmeI TCCRAC 4 cut(s) 1106, 2809, 3384, 3980
Mph1103I ATGCAT 4 cut(s) 2488, 2959, 4857, 5934
MroI TCCGGA 1 cut(s) 2850
MroXI GAANNNNTTC 2 cut(s) 1092, 1916
MseI TTAA 7 cut(s) 840, 849, 2396, 3309, 4725, 5373, 5832
MslI CAYNNNNRTG 5 cut(s) 1020, 1206, 1751, 2348, 5478
MspA1I CMGCKG 3 cut(s) 3287, 3329, 4026
MspI CCGG 3 cut(s) 2612, 2851, 2939
MunI CAATTG 2 cut(s) 1562, 3528
Mva1269I GAATGC 2 cut(s) 3011, 5656
MvnI CGCG 3 cut(s) 1948, 1987, 4135
NarI GGCGCC 1 cut(s) 171
NciI CCSGG 2 cut(s) 2612, 2940
NcoI CCATGG 2 cut(s) 525, 5810
NdeI CATATG 2 cut(s) 607, 3829
NheI GCTAGC 2 cut(s) 1031, 5527
NmeAIII GCCGAG 4 cut(s) 296, 307, 1672, 3650
NmuCI GTSAC 7 cut(s) 781, 1995, 2807, 4294, 5021, 5536, 5690
NsbI TGCGCA 1 cut(s) 1358
NsiI ATGCAT 4 cut(s) 2488, 2959, 4857, 5934
NspI RCATGY 4 cut(s) 1482, 1903, 2486, 5573
NspV TTCGAA 1 cut(s) 3790
PaeR7I CTCGAG 2 cut(s) 459, 2458
PagI TCATGA 1 cut(s) 4843
PaqCI CACCTGC 1 cut(s) 5140
PasI CCCWGGG 1 cut(s) 4161
PceI AGGCCT 2 cut(s) 952, 3040
PciSI GCTCTTC 1 cut(s) 5292
PcsI WCGNNNNNNNCGW 2 cut(s) 309, 4738
PctI GAATGC 2 cut(s) 3011, 5656
PdmI GAANNNNTTC 2 cut(s) 1092, 1916
PflFI GACNNNGTC 1 cut(s) 583
PflMI CCANNNNNTGG 3 cut(s) 398, 3833, 4805
PfoI TCCNGGA 3 cut(s) 2610, 3936, 4039
PkrI GCNGC 8 cut(s) 1308, 1970, 2466, 2777, 3262, 4489, 5635, 6100
PleI GAGTC 7 cut(s) 281, 1271, 4058, 4196, 4388, 4489, 4681
PluTI GGCGCC 1 cut(s) 174
PmaCI CACGTG 1 cut(s) 1208
PmlI CACGTG 1 cut(s) 1208
PpsI GAGTC 7 cut(s) 281, 1271, 4058, 4196, 4388, 4489, 4681
Ppu21I YACGTR 2 cut(s) 1208, 2843
PpuMI RGGWCCY 1 cut(s) 1609
PshBI ATTAAT 1 cut(s) 4725
PsiI TTATAA 2 cut(s) 1803, 5115
Psp124BI GAGCTC 4 cut(s) 43, 1653, 3758, 4972
Psp5II RGGWCCY 1 cut(s) 1609
PspCI CACGTG 1 cut(s) 1208
PspEI GGTNACC 1 cut(s) 2673
PspFI CCCAGC 3 cut(s) 889, 1878, 1904
PspOMI GGGCCC 2 cut(s) 69, 6033
PspPPI RGGWCCY 1 cut(s) 1609
PspXI VCTCGAGB 2 cut(s) 459, 2458
PstI CTGCAG 2 cut(s) 941, 6100
PstNI CAGNNNCTG 3 cut(s) 2893, 3254, 3266
PsuI RGATCY 7 cut(s) 673, 1408, 2494, 2878, 2947, 2983, 5290
PsyI GACNNNGTC 1 cut(s) 583
RsaI GTAC 9 cut(s) 1197, 1337, 1429, 1525, 2670, 4340, 4383, 5175, 5985
RsaNI GTAC 9 cut(s) 1196, 1336, 1428, 1524, 2669, 4339, 4382, 5174, 5984
RseI CAYNNNNRTG 5 cut(s) 1020, 1206, 1751, 2348, 5478
SacI GAGCTC 4 cut(s) 43, 1653, 3758, 4972
SapI GCTCTTC 1 cut(s) 5292
SaqAI TTAA 7 cut(s) 840, 849, 2396, 3309, 4725, 5373, 5832
SatI GCNGC 8 cut(s) 1307, 1969, 2465, 2776, 3261, 4488, 5634, 6099
SchI GAGTC 7 cut(s) 282, 1271, 4058, 4196, 4388, 4489, 4682
SexAI ACCWGGT 2 cut(s) 5230, 6129
SfcI CTRYAG 6 cut(s) 937, 2107, 3468, 4533, 4695, 6096
SfoI GGCGCC 1 cut(s) 172
Sfr274I CTCGAG 2 cut(s) 459, 2458
SfuI TTCGAA 1 cut(s) 3790
SlaI CTCGAG 2 cut(s) 459, 2458
SmiMI CAYNNNNRTG 5 cut(s) 1020, 1206, 1751, 2348, 5478
SmlI CTYRAG 7 cut(s) 459, 1301, 1838, 2458, 4370, 4526, 4822
SmoI CTYRAG 7 cut(s) 459, 1301, 1838, 2458, 4370, 4526, 4822
SpeI ACTAGT 1 cut(s) 785
SseBI AGGCCT 2 cut(s) 952, 3040
SspDI GGCGCC 1 cut(s) 170
SspI AATATT 1 cut(s) 2338
SstI GAGCTC 4 cut(s) 43, 1653, 3758, 4972
StuI AGGCCT 2 cut(s) 952, 3040
StyI CCWWGG 7 cut(s) 525, 696, 1604, 2859, 3041, 3151, 5810
TaiI ACGT 9 cut(s) 96, 306, 1210, 2829, 2845, 4240, 4523, 5103, 5327
TaqII GACCGA 4 cut(s) 280, 4479, 4589, 5555
TatI WGTACW 4 cut(s) 1335, 1427, 1523, 4338
TauI GCSGC 1 cut(s) 1971
Tru1I TTAA 7 cut(s) 840, 849, 2396, 3309, 4725, 5373, 5832
Tru9I TTAA 7 cut(s) 840, 849, 2396, 3309, 4725, 5373, 5832
TseFI GTSAC 7 cut(s) 781, 1995, 2807, 4294, 5021, 5536, 5690
TseI GCWGC 7 cut(s) 1306, 2464, 2775, 3260, 4487, 5633, 6098
Tsp45I GTSAC 7 cut(s) 781, 1995, 2807, 4294, 5021, 5536, 5690
Tth111I GACNNNGTC 1 cut(s) 583
Van91I CCANNNNNTGG 3 cut(s) 398, 3833, 4805
VneI GTGCAC 1 cut(s) 5672
VspI ATTAAT 1 cut(s) 4725
XbaI TCTAGA 2 cut(s) 2510, 4067
XceI RCATGY 4 cut(s) 1482, 1903, 2486, 5573
XcmI CCANNNNNNNNNTGG 3 cut(s) 1538, 1824, 4566
XhoI CTCGAG 2 cut(s) 459, 2458
XmaJI CCTAGG 2 cut(s) 3041, 3151
XmnI GAANNNNTTC 2 cut(s) 1092, 1916
ZraI GACGTC 1 cut(s) 304
Zsp2I ATGCAT 4 cut(s) 2488, 2959, 4857, 5934
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.