Rh5CG162000

PB1 domain

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr5C
OG0004019
Physical Location & Seq
Reverse (-)
14945302 .. 14949511
4210 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh5CG162000.1

Sequence Viewer

Length: 3384 bp
ATGCTCATGTACCATTTTTACTGCGTTTTGTATTGGTTGTTTGTCCTGGTGAAGTTTTACCTTATGGATAGAAATCTGGGTAAAGGTGCAATGGATCAACCAAAAAACTACGATCAAGTTCGATACAGCACTGTTGAAACTAGAAACGACGGATATGGGTCTGCCAATCAACGGTTTTTCCTGGATCCATCAAGTAATATTAGCACTAATATGCGACCTCCAGAGTATAATGTTTCTGGTGGAACCAGACCTGTATTAAATTACTCTATTCAGACTGGCGAGGAATTTGCTCTTGAATTTATGCGGGAAAGGGTGAACTCGAGGCAGCATTTATTAGTCCCACATGCATCTGGGGATCCTAACAGTGCTTCTAGATATATGGGTCTAAAAGGCATACTTGGAATCAGCCATACAGGCTCAGAGAGTGGGTCCGATGTTTCTATGCTGAATTTGGTAGAGAAAGATCGTGTCCCGGAAAATGAAACAAAGGCATCTTCTCCTCTTGAAAATAAAAGTTACTATGATTCAGTACGGTTACCACAACCCTCATCAAGAAATGACATCAATCGAGGACTTTCTGGTTATTCCTCTGCAGGAGTGTCTGATAGCTCATCGAAAAAAGTGAAGTTCCTTTGCAGCTTTGGTGGTAAAATCTTGCCTCGTCCTAGTGACGGAAAGCTTAGATATGTTGGAGGTGAAACACGTATTATCCGGATAACCAAGGATATCGTTTGGCAGGATCTTATGCAGAAACTGTTGGCAGTTTATGACCAAACTCATACAATAAAGTATCAGCTTCCGGGCGAGGATCTTGATGCATTAGTTTCGGTTTCTTCTGATGAGGATCTGCAAAATATGATGGAGGAATGCACTATTCTACAGGATGGAGGATCACAGAGGCCTAGGATGTTCCTGTTTTCTAGTCTTGATTTAGAGGAATCTCAATTTGGCCCTGAAAGCGTGGAGGGGGATTCTGAGATTCAATATGTTGTTGCTGTAAACGGGATGGACCTAGGTTCAAAAAAGAATTCTATTGCTTTGGCAAGTTCTTCAGGCAACAATTTGGAAGAGTTACTCAGTCTAAATGTTACAAGGGGCAGCACTCACACAGTGCTTGATGCAGCTCCTGCTGGCACTGTTCCTTCAGCGGTAGATGTGCCTTCCTCGGTTAATCAAGCTTCTCATTCAGCGGTTCCTGGTTCTTCAAGTGCTTATGAATCCAACTCACAGCTTTATCAGGGACAAAAAATGCATAGTGGAGACACCCGACAGTATCCATCGTCAGCTTTGAACCCTGTGGAGAGCTTCCCTGCAAAAGATGAACAGACTACAGTGCTCTCATCTGTTCCAGTGCAATATGATTTTGGTTCTCAACCTCCTAATTACGCAATTGGAGAAAATATAGGTTCTATGCCTATCTATGGACAGCCGATTCAACAAGGGGGTCTGATTGAAGATAAGCTATATGCTGGAATACATGGGCAAGATTCAGAATTACCCATAAAGGAGATGAAACTGAAAAGAGATAGCTCGGCCCAAAAGATAAATGAAGCTGAGAAAGTTCAATCTTTAGAGAAGGATACTCCACAGAAGGAAGCAAGAATGACAAGAGAGAGCTCTCTCCAGAAGCTAAATGAAACTGATAAAGTTCGAAGTTTAGAAAATGAGAAGACTGTTTCTGTAAATCCATATGATGGTTCTGTTCCAAATTATATTTCTAGGGATGAAGTTTCAGTTGCCAATTCAGTAGCAGAGACAGGTTCACCCTTATTGGCTACAAGAAGCAACAAAAAGCTCCAGGAACCTCGACAGAATCCAACAACCTCTGAAGATGTAAATGATGGGAAAAAGAACAATGAAGATGACCGTTTCCACACAACAACCAGCGGACTGTCTATTCCTGGATATGCTGGCTCAGAAGCTGACTCTAGATATGCTGGCTCTGAAGTTGACTCTAGATATGCTGGCTCTGAGGTTGATTCTATGGATTTTAGCTATCTTGAACAACCTGTGGTTCCTCCGCGTGTCTATCATTCAGAACGAATCCCCAGGGAGCAATCAGAACTGAACCGTCTATCAAAATCTGGGGACTCTTTTGGTTCGCCGTTTATGGTAGCTCAGGCACGTCCTGATCACAAACAACCAATCATGGAATCTGTTGAGAAATTGCATGATGAGAATGTCACTTTGCAGTCTGAGCAGTCTGTCTTACCTCCAAAACTACTGTACAAAATTCCTCAGACAGTTGAAGAGGGGCTTGAGCGAAAGGTACAAAAACCTGACTCAATACATCCAGTTGGTAATTCTGGTGATGGCCGTGAAACGGGCAGGCTAAAGGATAACTACGGAGACCGAAGTATCAGTGATAAACAAGCAGCGCTGACTCAACTAAGAGCTGGTCAAGAAACGTCTCTCAAGCCTACAGATGATTCTGCTTCAGTTCCACCAGAATTTGAATGGACTGAGATAACTGCCAGTAAGGACTACGGGAATAATGGCAAGGTTTATGTAAATCTTGTGGCTCGGAAAGAGAACCCAATTACAGGTGGTGGTAATGGCGAGTCTACTGTTGGTGTTGGCACTACAGAGCACGGGGATATTCTTATTGATATTAATGATCGTTTTCCCCGTGATTTTCTGTCTGATATATTTTCCAAAGCTAGGATAGACCAGTCTGGTGTCAGTCCACTTCCTGGGGATGGAACTGGCTTGAGCTTGAACATGGAGAATCATGAACCTATGCATTGGTCATACTTCCGAAATTTGGCTCAGAATGAATTTGTAAGAAAAGATGTCTCCCTTATGGATCAGGACCATCTGGGTTTCTCATCCCCACTGACAGGTATTGGAGAAGGAGCTCCAGTTGATTACAGCTATCCACCTTTGAAGTCTGCTGGAGTTGTTTTTGGTCACACTGAATCACATATCAGTTTTGATGAGGATATTCGGCAAGATTTGTCATCTATAACTGGACCCACCACCATAAATGTAAAGTCAGATTATAATCCTTCTCTACCTGAGGGAATTGAAAGTGAGCAGGTGGATGGGGTAAATCATAGAGTACGAGAATCAGAATATGAGGATGACAAGCTAGAGAACAAGAACACAGCTGGACCACCTGGTGATCTTCCTCTGGAAGATTTTGATATCACTACCTTGCAGATCATAAAAAATGAAGATCTTGAAGAGCTTAGGGAATTGGGTTCTGGCACGTTTGGGACTGTTTATCATGGAAAGTGGAGGGGAACAGATGTTGCCATTAAGCGAATAAAAAAGAGCTGTTTCACAGGGCGCTCATCAGAGCAAGAAAGATTGGTGAGTCGGAGCATATTGTTTTCTTATTCAATAACAACTAAGCACGTAGAGTCAGCTTCAGTCATGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
PB1 Pkinase_Tyr

Protein Analysis

1127

Amino Acids

124.3

Weight (kDa)

5.01

Isoelectric Point (pI)

48.32

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PB1 PF00564 224 - 307 2.5e-19 PB1 domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 3003
AarI CACCTGC 1 cut(s) 3028
Acc36I ACCTGC 1 cut(s) 3028
AccB7I CCANNNNNTGG 2 cut(s) 1694, 2693
AccI GTMKAC 1 cut(s) 2564
AccII CGCG 1 cut(s) 2023
AccIII TCCGGA 1 cut(s) 711
AciI CCGC 5 cut(s) 304, 1148, 1190, 1887, 2021
AcoI YGGCCR 1 cut(s) 2314
AcsI RAATTY 8 cut(s) 284, 296, 448, 1027, 2232, 2450, 2761, 2777
AcuI CTGAAG 6 cut(s) 1035, 1128, 1848, 1965, 2421, 3357
AdeI CACNNNGTG 2 cut(s) 1111, 3122
AfaI GTAC 5 cut(s) 11, 531, 2228, 2271, 3063
AfeI AGCGCT 1 cut(s) 2379
AflIII ACRYGT 1 cut(s) 701
AjiI CACGTC 1 cut(s) 2126
AjnI CCWGG 8 cut(s) 45, 180, 1195, 1797, 1900, 2048, 2692, 3118
AjuI GAANNNNNNNTTGG 4 cut(s) 930, 962, 1699, 1731
Alw21I GWGCWC 4 cut(s) 1338, 1619, 2592, 2860
Alw26I GTCTC 5 cut(s) 1254, 1748, 2343, 2415, 2800
AlwNI CAGNNNCTG 3 cut(s) 754, 1127, 1922
Ama87I CYCGRG 1 cut(s) 319
Aor13HI TCCGGA 1 cut(s) 711
Aor51HI AGCGCT 1 cut(s) 2379
AoxI GGCC 4 cut(s) 899, 949, 1533, 2314
ApeKI GCWGC 5 cut(s) 325, 636, 1098, 1121, 2375
ApoI RAATTY 8 cut(s) 284, 296, 448, 1027, 2232, 2450, 2761, 2777
AseI ATTAAT 1 cut(s) 2613
AspA2I CCTAGG 2 cut(s) 902, 1012
AspLEI GCGC 2 cut(s) 2380, 3294
AspS9I GGNCC 7 cut(s) 429, 950, 1009, 1534, 2812, 2972, 3113
AsuC2I CCSGG 2 cut(s) 473, 801
AsuHPI GGTGA 7 cut(s) 61, 325, 707, 1755, 2321, 3134, 3328
AsuII TTCGAA 1 cut(s) 1651
AvaI CYCGRG 1 cut(s) 319
AvaII GGWCC 5 cut(s) 429, 1009, 2812, 2972, 3113
AvrII CCTAGG 2 cut(s) 902, 1012
AxyI CCTNAGG 1 cut(s) 3018
BaeI ACNNNNGTAYC 2 cut(s) 2341, 2374
BamHI GGATCC 2 cut(s) 184, 355
BanII GRGCYC 2 cut(s) 1619, 2860
BbsI GAAGAC 1 cut(s) 1676
Bbv12I GWGCWC 4 cut(s) 1338, 1619, 2592, 2860
BbvI GCAGC 5 cut(s) 337, 648, 1110, 1133, 2387
BceAI ACGGC 2 cut(s) 2089, 2301
BcgI CGANNNNNNTGC 4 cut(s) 269, 303, 807, 841
BciT130I CCWGG 8 cut(s) 47, 182, 1197, 1799, 1902, 2050, 2694, 3120
BciVI GTATCC 2 cut(s) 1284, 1573
BclI TGATCA 1 cut(s) 2131
BcnI CCSGG 2 cut(s) 473, 801
BcoDI GTCTC 5 cut(s) 1254, 1748, 2343, 2415, 2800
BfmI CTRYAG 5 cut(s) 591, 878, 1329, 2421, 2583
BfoI RGCGCY 2 cut(s) 2381, 3295
BfuAI ACCTGC 1 cut(s) 3028
BfuI GTATCC 2 cut(s) 1284, 1573
BglI GCCNNNNNGGC 1 cut(s) 414
BglII AGATCT 1 cut(s) 3178
BisI GCNGC 5 cut(s) 326, 637, 1099, 1122, 2376
BlnI CCTAGG 2 cut(s) 902, 1012
BlsI GCNGC 5 cut(s) 327, 638, 1100, 1123, 2377
Bme18I GGWCC 5 cut(s) 429, 1009, 2812, 2972, 3113
BmeT110I CYCGRG 1 cut(s) 319
BmgBI CACGTC 1 cut(s) 2126
BmgT120I GGNCC 7 cut(s) 429, 950, 1009, 1534, 2812, 2972, 3113
BmiI GGNNCC 8 cut(s) 186, 244, 357, 430, 1194, 1803, 2016, 2974
BmsI GCATC 4 cut(s) 356, 500, 805, 1108
BpiI GAAGAC 1 cut(s) 1676
BpmI CTGGAG 5 cut(s) 204, 1607, 1781, 2844, 2916
Bpu10I CCTNAGC 2 cut(s) 2118, 3191
Bpu14I TTCGAA 1 cut(s) 1651
BpuEI CTTGAG 3 cut(s) 2279, 2399, 2731
BpuMI CCSGG 2 cut(s) 473, 801
BsaAI YACGTR 2 cut(s) 704, 3361
BsaBI GATNNNNATC 3 cut(s) 72, 843, 3003
BsaI GGTCTC 1 cut(s) 2343
BsaJI CCNNGG 7 cut(s) 720, 902, 1012, 1164, 2048, 2049, 2693
BsaWI WCCGGW 1 cut(s) 711
BsaXI ACNNNNNCTCC 2 cut(s) 2847, 2877
Bse1I ACTGG 8 cut(s) 280, 1349, 2294, 2475, 2671, 2710, 2861, 2974
Bse21I CCTNAGG 1 cut(s) 3018
Bse3DI GCAATG 1 cut(s) 96
Bse8I GATNNNNATC 3 cut(s) 72, 843, 3003
BseAI TCCGGA 1 cut(s) 711
BseBI CCWGG 8 cut(s) 47, 182, 1197, 1799, 1902, 2050, 2694, 3120
BseDI CCNNGG 7 cut(s) 720, 902, 1012, 1164, 2048, 2049, 2693
BseGI GGATG 9 cut(s) 889, 912, 1011, 1729, 2290, 2704, 2828, 3049, 3088
BseJI GATNNNNATC 3 cut(s) 72, 843, 3003
BseMI GCAATG 1 cut(s) 96
BseNI ACTGG 8 cut(s) 280, 1349, 2294, 2475, 2671, 2710, 2861, 2974
BseRI GAGGAG 1 cut(s) 489
BseXI GCAGC 5 cut(s) 337, 648, 1110, 1133, 2387
Bsh1236I CGCG 1 cut(s) 2023
BshFI GGCC 4 cut(s) 901, 951, 1535, 2316
BsiHKAI GWGCWC 4 cut(s) 1338, 1619, 2592, 2860
BsiHKCI CYCGRG 1 cut(s) 319
BsiSI CCGG 3 cut(s) 473, 712, 800
BslFI GGGAC 5 cut(s) 323, 455, 1254, 2102, 3232
BsmAI GTCTC 5 cut(s) 1254, 1748, 2343, 2415, 2800
BsmBI CGTCTC 1 cut(s) 2415
BsmFI GGGAC 5 cut(s) 323, 455, 1254, 2102, 3232
BsmI GAATGC 1 cut(s) 872
BsnI GGCC 4 cut(s) 901, 951, 1535, 2316
Bso31I GGTCTC 1 cut(s) 2343
BsoBI CYCGRG 1 cut(s) 319
Bsp119I TTCGAA 1 cut(s) 1651
Bsp1286I GDGCHC 4 cut(s) 1338, 1619, 2592, 2860
Bsp13I TCCGGA 1 cut(s) 711
Bsp1407I TGTACA 1 cut(s) 2226
BspACI CCGC 5 cut(s) 304, 1148, 1190, 1887, 2021
BspANI GGCC 4 cut(s) 901, 951, 1535, 2316
BspEI TCCGGA 1 cut(s) 711
BspFNI CGCG 1 cut(s) 2023
BspHI TCATGA 1 cut(s) 2731
BspLI GGNNCC 8 cut(s) 186, 244, 357, 430, 1194, 1803, 2016, 2974
BspMAI CTGCAG 1 cut(s) 595
BspMI ACCTGC 1 cut(s) 3028
BspQI GCTCTTC 1 cut(s) 3180
BspT104I TTCGAA 1 cut(s) 1651
BspTNI GGTCTC 1 cut(s) 2343
BsrDI GCAATG 1 cut(s) 96
BsrGI TGTACA 1 cut(s) 2226
BsrI ACTGG 8 cut(s) 280, 1349, 2294, 2475, 2671, 2710, 2861, 2974
BssECI CCNNGG 7 cut(s) 720, 902, 1012, 1164, 2048, 2049, 2693
BssT1I CCWWGG 3 cut(s) 720, 902, 1012
Bst2UI CCWGG 8 cut(s) 47, 182, 1197, 1799, 1902, 2050, 2694, 3120
Bst6I CTCTTC 3 cut(s) 1062, 2244, 3180
BstAPI GCANNNNNTGC 1 cut(s) 1127
BstAUI TGTACA 1 cut(s) 2226
BstBAI YACGTR 2 cut(s) 704, 3361
BstBI TTCGAA 1 cut(s) 1651
BstC8I GCNNGC 5 cut(s) 1132, 1912, 1939, 1966, 2330
BstEII GGTNACC 1 cut(s) 534
BstF5I GGATG 9 cut(s) 889, 912, 1011, 1729, 2290, 2704, 2828, 3049, 3088
BstFNI CGCG 1 cut(s) 2023
BstH2I RGCGCY 2 cut(s) 2381, 3295
BstHHI GCGC 2 cut(s) 2380, 3294
BstMAI GTCTC 5 cut(s) 1254, 1748, 2343, 2415, 2800
BstMWI GCNNNNNNNGC 4 cut(s) 414, 957, 1127, 2197
BstNI CCWGG 8 cut(s) 47, 182, 1197, 1799, 1902, 2050, 2694, 3120
BstNSI RCATGY 1 cut(s) 347
BstPI GGTNACC 1 cut(s) 534
BstSFI CTRYAG 5 cut(s) 591, 878, 1329, 2421, 2583
BstUI CGCG 1 cut(s) 2023
BstV1I GCAGC 5 cut(s) 337, 648, 1110, 1133, 2387
BstV2I GAAGAC 1 cut(s) 1676
BstX2I RGATCY 6 cut(s) 184, 355, 739, 808, 844, 3178
BstYI RGATCY 6 cut(s) 184, 355, 739, 808, 844, 3178
Bsu36I CCTNAGG 1 cut(s) 3018
BsuI GTATCC 2 cut(s) 1284, 1573
BsuRI GGCC 4 cut(s) 901, 951, 1535, 2316
BtrI CACGTC 1 cut(s) 2126
BtsCI GGATG 9 cut(s) 889, 912, 1011, 1729, 2290, 2704, 2828, 3049, 3088
BtsIMutI CAGTG 9 cut(s) 129, 370, 1116, 1134, 1338, 1356, 2368, 2834, 2913
BveI ACCTGC 1 cut(s) 3028
Cac8I GCNNGC 5 cut(s) 1132, 1912, 1939, 1966, 2330
CaiI CAGNNNCTG 3 cut(s) 754, 1127, 1922
CciI TCATGA 1 cut(s) 2731
CfoI GCGC 2 cut(s) 2380, 3294
Cfr13I GGNCC 7 cut(s) 429, 950, 1009, 1534, 2812, 2972, 3113
CsiI ACCWGGT 1 cut(s) 3118
Csp6I GTAC 5 cut(s) 10, 530, 2227, 2270, 3062
CviAII CATG 9 cut(s) 7, 344, 1478, 2149, 2171, 2722, 2732, 3230, 3379
CviQI GTAC 5 cut(s) 10, 530, 2227, 2270, 3062
DraIII CACNNNGTG 2 cut(s) 1111, 3122
EaeI YGGCCR 1 cut(s) 2314
Eam1104I CTCTTC 3 cut(s) 1062, 2244, 3180
EarI CTCTTC 3 cut(s) 1062, 2244, 3180
Ecl136II GAGCTC 2 cut(s) 1617, 2858
Eco130I CCWWGG 3 cut(s) 720, 902, 1012
Eco147I AGGCCT 1 cut(s) 901
Eco24I GRGCYC 2 cut(s) 1619, 2860
Eco31I GGTCTC 1 cut(s) 2343
Eco32I GATATC 2 cut(s) 727, 3148
Eco47I GGWCC 5 cut(s) 429, 1009, 2812, 2972, 3113
Eco47III AGCGCT 1 cut(s) 2379
Eco53kI GAGCTC 2 cut(s) 1617, 2858
Eco57I CTGAAG 6 cut(s) 1035, 1128, 1848, 1965, 2421, 3357
Eco81I CCTNAGG 1 cut(s) 3018
Eco88I CYCGRG 1 cut(s) 319
Eco91I GGTNACC 1 cut(s) 534
EcoICRI GAGCTC 2 cut(s) 1617, 2858
EcoO65I GGTNACC 1 cut(s) 534
EcoRI GAATTC 1 cut(s) 1027
EcoRII CCWGG 8 cut(s) 45, 180, 1195, 1797, 1900, 2048, 2692, 3118
EcoRV GATATC 2 cut(s) 727, 3148
EcoT14I CCWWGG 3 cut(s) 720, 902, 1012
EcoT22I ATGCAT 4 cut(s) 349, 820, 1254, 2745
EcoT38I GRGCYC 2 cut(s) 1619, 2860
ErhI CCWWGG 3 cut(s) 720, 902, 1012
Esp3I CGTCTC 1 cut(s) 2415
FaeI CATG 9 cut(s) 10, 347, 1481, 2152, 2174, 2725, 2735, 3233, 3382
FalI AAGNNNNNCTT 4 cut(s) 381, 413, 2241, 2273
FaqI GGGAC 5 cut(s) 323, 455, 1254, 2102, 3232
FatI CATG 9 cut(s) 6, 343, 1477, 2148, 2170, 2721, 2731, 3229, 3378
FauI CCCGC 1 cut(s) 297
FauNDI CATATG 1 cut(s) 1690
FbaI TGATCA 1 cut(s) 2131
FblI GTMKAC 1 cut(s) 2564
Fnu4HI GCNGC 5 cut(s) 326, 637, 1099, 1122, 2376
FokI GGATG 9 cut(s) 896, 919, 1018, 1736, 2277, 2711, 2815, 3056, 3095
FriOI GRGCYC 2 cut(s) 1619, 2860
Fsp4HI GCNGC 5 cut(s) 326, 637, 1099, 1122, 2376
GlaI GCGC 2 cut(s) 2379, 3293
GluI GCNGC 5 cut(s) 326, 637, 1099, 1122, 2376
GsuI CTGGAG 5 cut(s) 204, 1607, 1781, 2844, 2916
HaeII RGCGCY 2 cut(s) 2381, 3295
HaeIII GGCC 4 cut(s) 901, 951, 1535, 2316
HapII CCGG 3 cut(s) 473, 712, 800
HhaI GCGC 2 cut(s) 2380, 3294
Hin1II CATG 9 cut(s) 10, 347, 1481, 2152, 2174, 2725, 2735, 3233, 3382
Hin6I GCGC 2 cut(s) 2378, 3292
HinP1I GCGC 2 cut(s) 2378, 3292
HincII GTYRAC 1 cut(s) 1951
HindII GTYRAC 1 cut(s) 1951
HindIII AAGCTT 2 cut(s) 677, 1176
HpaII CCGG 3 cut(s) 473, 712, 800
HphI GGTGA 7 cut(s) 61, 325, 707, 1755, 2321, 3134, 3328
Hpy166II GTNNAC 6 cut(s) 316, 1000, 1763, 1951, 2565, 2687
Hpy8I GTNNAC 6 cut(s) 316, 1000, 1763, 1951, 2565, 2687
Hpy99I CGWCG 1 cut(s) 152
HpyAV CCTTC 6 cut(s) 1152, 1170, 1570, 1585, 2846, 3018
HpyCH4IV ACGT 5 cut(s) 703, 2125, 2408, 3212, 3360
HpyF10VI GCNNNNNNNGC 4 cut(s) 414, 957, 1127, 2197
HpySE526I ACGT 5 cut(s) 703, 2125, 2408, 3212, 3360
Hsp92II CATG 9 cut(s) 10, 347, 1481, 2152, 2174, 2725, 2735, 3233, 3382
HspAI GCGC 2 cut(s) 2378, 3292
Kpn2I TCCGGA 1 cut(s) 711
Ksp22I TGATCA 1 cut(s) 2131
LguI GCTCTTC 1 cut(s) 3180
LmnI GCTCC 6 cut(s) 1129, 1800, 2053, 2855, 2863, 3324
Lsp1109I GCAGC 5 cut(s) 337, 648, 1110, 1133, 2387
LweI GCATC 4 cut(s) 356, 500, 805, 1108
MabI ACCWGGT 1 cut(s) 3118
MaeII ACGT 5 cut(s) 703, 2125, 2408, 3212, 3360
MaeIII GTNAC 7 cut(s) 515, 534, 668, 1071, 1087, 2182, 2909
MfeI CAATTG 1 cut(s) 1389
MflI RGATCY 6 cut(s) 184, 355, 739, 808, 844, 3178
MhlI GDGCHC 4 cut(s) 1338, 1619, 2592, 2860
MlyI GAGTC 8 cut(s) 1919, 1946, 2084, 2276, 2377, 2570, 3328, 3374
MmeI TCCRAC 4 cut(s) 670, 1245, 1841, 3302
Mph1103I ATGCAT 4 cut(s) 349, 820, 1254, 2745
MroI TCCGGA 1 cut(s) 711
MseI TTAA 4 cut(s) 257, 1170, 2613, 3261
MslI CAYNNNNRTG 1 cut(s) 209
MspA1I CMGCKG 4 cut(s) 1148, 1190, 1887, 3110
MspI CCGG 3 cut(s) 473, 712, 800
MunI CAATTG 1 cut(s) 1389
Mva1269I GAATGC 1 cut(s) 872
MvaI CCWGG 8 cut(s) 47, 182, 1197, 1799, 1902, 2050, 2694, 3120
MvnI CGCG 1 cut(s) 2023
MwoI GCNNNNNNNGC 4 cut(s) 414, 957, 1127, 2197
NciI CCSGG 2 cut(s) 473, 801
NdeI CATATG 1 cut(s) 1690
NlaIII CATG 9 cut(s) 10, 347, 1481, 2152, 2174, 2725, 2735, 3233, 3382
NlaIV GGNNCC 8 cut(s) 186, 244, 357, 430, 1194, 1803, 2016, 2974
NmeAIII GCCGAG 1 cut(s) 1511
NmuCI GTSAC 3 cut(s) 668, 2182, 2909
NsiI ATGCAT 4 cut(s) 349, 820, 1254, 2745
NspI RCATGY 1 cut(s) 347
NspV TTCGAA 1 cut(s) 1651
PaeR7I CTCGAG 1 cut(s) 319
PagI TCATGA 1 cut(s) 2731
PaqCI CACCTGC 1 cut(s) 3028
PasI CCCWGGG 1 cut(s) 2049
PceI AGGCCT 1 cut(s) 901
PciSI GCTCTTC 1 cut(s) 3180
PcsI WCGNNNNNNNCGW 1 cut(s) 2626
PctI GAATGC 1 cut(s) 872
PflMI CCANNNNNTGG 2 cut(s) 1694, 2693
PfoI TCCNGGA 4 cut(s) 180, 471, 1797, 1900
PkrI GCNGC 5 cut(s) 327, 638, 1100, 1123, 2377
PleI GAGTC 8 cut(s) 1919, 1946, 2084, 2276, 2377, 2569, 3327, 3373
PpsI GAGTC 8 cut(s) 1919, 1946, 2084, 2276, 2377, 2569, 3327, 3373
Ppu21I YACGTR 2 cut(s) 704, 3361
PshBI ATTAAT 1 cut(s) 2613
PsiI TTATAA 1 cut(s) 3003
Psp124BI GAGCTC 2 cut(s) 1619, 2860
Psp6I CCWGG 8 cut(s) 45, 180, 1195, 1797, 1900, 2048, 2692, 3118
PspEI GGTNACC 1 cut(s) 534
PspGI CCWGG 8 cut(s) 45, 180, 1195, 1797, 1900, 2048, 2692, 3118
PspN4I GGNNCC 8 cut(s) 186, 244, 357, 430, 1194, 1803, 2016, 2974
PspPI GGNCC 7 cut(s) 429, 950, 1009, 1534, 2812, 2972, 3113
PspXI VCTCGAGB 1 cut(s) 319
PstI CTGCAG 1 cut(s) 595
PstNI CAGNNNCTG 3 cut(s) 754, 1127, 1922
PsuI RGATCY 6 cut(s) 184, 355, 739, 808, 844, 3178
PvuII CAGCTG 1 cut(s) 3110
RsaI GTAC 5 cut(s) 11, 531, 2228, 2271, 3063
RsaNI GTAC 5 cut(s) 10, 530, 2227, 2270, 3062
RseI CAYNNNNRTG 1 cut(s) 209
SacI GAGCTC 2 cut(s) 1619, 2860
SapI GCTCTTC 1 cut(s) 3180
SaqAI TTAA 4 cut(s) 257, 1170, 2613, 3261
SatI GCNGC 5 cut(s) 326, 637, 1099, 1122, 2376
Sau96I GGNCC 7 cut(s) 429, 950, 1009, 1534, 2812, 2972, 3113
SchI GAGTC 8 cut(s) 1919, 1946, 2084, 2276, 2377, 2570, 3328, 3374
SduI GDGCHC 4 cut(s) 1338, 1619, 2592, 2860
SexAI ACCWGGT 1 cut(s) 3118
SfaNI GCATC 4 cut(s) 356, 500, 805, 1108
SfcI CTRYAG 5 cut(s) 591, 878, 1329, 2421, 2583
Sfr274I CTCGAG 1 cut(s) 319
SfuI TTCGAA 1 cut(s) 1651
SinI GGWCC 5 cut(s) 429, 1009, 2812, 2972, 3113
SlaI CTCGAG 1 cut(s) 319
SmiMI CAYNNNNRTG 1 cut(s) 209
SmlI CTYRAG 4 cut(s) 319, 2258, 2414, 2710
SmoI CTYRAG 4 cut(s) 319, 2258, 2414, 2710
SseBI AGGCCT 1 cut(s) 901
SsiI CCGC 5 cut(s) 304, 1148, 1190, 1887, 2021
SspI AATATT 1 cut(s) 199
SstI GAGCTC 2 cut(s) 1619, 2860
StuI AGGCCT 1 cut(s) 901
StyI CCWWGG 3 cut(s) 720, 902, 1012
TaiI ACGT 5 cut(s) 706, 2128, 2411, 3215, 3363
TaqI TCGA 6 cut(s) 121, 320, 568, 614, 1651, 1807
TaqII GACCGA 1 cut(s) 2367
TatI WGTACW 1 cut(s) 2226
Tru1I TTAA 4 cut(s) 257, 1170, 2613, 3261
Tru9I TTAA 4 cut(s) 257, 1170, 2613, 3261
TscAI CASTG 9 cut(s) 136, 370, 1116, 1141, 1338, 1356, 2368, 2841, 2920
TseFI GTSAC 3 cut(s) 668, 2182, 2909
TseI GCWGC 5 cut(s) 325, 636, 1098, 1121, 2375
Tsp45I GTSAC 3 cut(s) 668, 2182, 2909
TspGWI ACGGA 3 cut(s) 165, 687, 2361
TspRI CASTG 9 cut(s) 136, 370, 1116, 1141, 1338, 1356, 2368, 2841, 2920
Van91I CCANNNNNTGG 2 cut(s) 1694, 2693
VpaK11BI GGWCC 5 cut(s) 429, 1009, 2812, 2972, 3113
VspI ATTAAT 1 cut(s) 2613
XapI RAATTY 8 cut(s) 284, 296, 448, 1027, 2232, 2450, 2761, 2777
XbaI TCTAGA 3 cut(s) 371, 1928, 1955
XceI RCATGY 1 cut(s) 347
XcmI CCANNNNNNNNNTGG 1 cut(s) 2454
XhoI CTCGAG 1 cut(s) 319
XmaJI CCTAGG 2 cut(s) 902, 1012
XmiI GTMKAC 1 cut(s) 2564
Zsp2I ATGCAT 4 cut(s) 349, 820, 1254, 2745
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.