pycom05g22660

PB1 domain

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr5
OG0004019
Physical Location & Seq
Forward (+)
24912044 .. 24914041
1998 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom05g22660.1

Sequence Viewer

Length: 1998 bp
ATGCTTACGTGTCATATTTACTGCTTGTTTGCTTGGATGTTTATCCATGTGACTTTAGACAGCATGGATAGAAATATGGGTAGAGGTGCAATGGATCAACACAAAAACTATGAGCACGTTCGCTACAATAATGTTGAAGCTAGAAACGACGGAAATGGGTCATCCAATCAAAGGTACTTCCAGGATCCATCAAGTAATGCTAGTACTAACATGAGACCTCCAGATTACAATGTTGCTGTTGGAGCCAGGCCTGTGTTGAATTACTCCATTCAGACCGGTGAGGAATTTGCTCTTGAATTCATGAGGGAAAGAGTGAACCCAAGGCAACATTTAGCTTCTGGGGATCCTAATAGTGCTCCTAACTATATGGATCTAAAGGGCTTACTGGGAATTAGCCATACAGGTTCAGAAAGTGGGTCTGATACTTCGTTACCAAACATGGTAGATAAAGGGCGTGTCCAGGAATTTGAAAGAAAGGCATCTTATGCACACGAAGATAAGAGTTACTTTGATTCGGTACGATTACCACAAACCTCATCAAGGAATGACAGCAATCGAGGACTTTATTATGCTTCTTCAGGAATGTCTGATAGCTCAGTGAGGAAAGTGAAGTTTCTCTGCAGCTTTGGTGGTAAAATTTTACCTCGTCCGAGTGATGGAAGGCTTAGATATGTTGGAGGCGAAACACGTATTATACGGTTAAACAGGGATGTCTTTTGGCAGGATCTGATGCAGAAAATGTTGACAATTTATGACCAAACTCGTGCAATAAAGTATCAGCTTCCCGGTGAGGATCTTGATGCACTTGTTTCTGTTTCATGTGATGAAGATCTGCAAAACATGATGGATGAGTTCAATGGGCTACAAGATGGGGGATCACAGAAACCTAGGGTGTTCCTGTTTTCTCATGTTGATTTGGAGGATTCTCAGTATGGTGTAGAAAGCGTGGATGGTGATTCTGAGATTCAGTATGTAGTTGCTGTAAATGGTATTGACTTAGGTTCAAGAAAGAATTCTATTGCTTTGGCAAGTTCTCCAGGCAACAACTTGGAAGAGTTGCTCAGTCTAAACGTTGCAAGGGAGAGCACTCAAGCAGTGCCTGATACAGTTGGTGCTGGCACAGCGCCTTCGGCACCAAATGTGCCTATCTCAACTGACCTTTCTTCTCAATCAGGATTTCCAGGTTCTTCAAGGGCTAATGAATCCAATTCACAACCATGCCAGGGGCAGATGATGCATAGTGGAGAAGCCAGACAACATCCAGTGTCAACTTTCCACTCTGTCCCCGGGAAAGATGGTCAGACTGCTGTGCCTTCATCGGTTCCATTACAATATGATTCTAGTTCTCTCACTTCACATCAAGCAACACCTGGAGGAAATATTGATTCAATGCCAATACACGGCCAATCCCCTCAACAGGGGGGATTGATTGAAGAGCAGCTATATGGTGGCATGCGTGGGCAAGGTTCAGAATTGCCCATAAAGGAGACAAAATTGAAAAAAAACAGCTCAGCAAAAAAGATAAGTGAACCTGAGAAAATTCAATCTTTAGAGAAGGAAGTTTCACCAAAGGAAGCAAGAATGAAGAGAGAGAGTTCTCTCCACAAGATAAATGAAAGCGATAAACCGAGGAATTTAGAAAATGAAAACACTGTTTCTCTCCCTTCATCTGACGGTTTAGTTCCCAATCATATTTCTAGGGATCAAGTTTCAGTTGCCAATTCAGCAGCAGAGACAGGATCACCTTTGTTGGCTTCAAGAAGCAGCAAAAAGCTCCAGGAACCTCGACAAAAACCAATCGCCTCTGAAGATGTAAATGATGGGAAAAAGATCAATGAAGATGACCAATTCCACACAACCAGTGGACAGTCTACTCCTGGATATGGTGGCTCTGAGGTTGATTCAATGGACTTTAGCTACCTTGAACCACCTGTGGCTCCTCAGCGTGTCTATCATTCTGAGCGGATCGATCAAGTCCCAGGGAACAAGCAGAATTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
PB1 Pkinase_Tyr

Protein Analysis

666

Amino Acids

73.03

Weight (kDa)

5.48

Isoelectric Point (pI)

51.03

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PB1 PF00564 219 - 301 2e-16 PB1 domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 1132
AccBSI CCGCTC 1 cut(s) 1963
AccI GTMKAC 1 cut(s) 1871
AciI CCGC 1 cut(s) 1963
AclI AACGTT 1 cut(s) 1071
AcoI YGGCCR 1 cut(s) 1402
AcsI RAATTY 7 cut(s) 284, 296, 464, 636, 1012, 1539, 1633
AcuI CTGAAG 2 cut(s) 561, 1827
AfaI GTAC 3 cut(s) 176, 205, 519
AfiI CCNNNNNNNGG 8 cut(s) 171, 540, 656, 1223, 1400, 1417, 1418, 1883
AflIII ACRYGT 2 cut(s) 8, 686
AgeI ACCGGT 1 cut(s) 275
AluBI AGCT 9 cut(s) 140, 335, 594, 624, 781, 1441, 1509, 1774, 1917
AluI AGCT 9 cut(s) 140, 335, 594, 624, 781, 1441, 1509, 1774, 1917
Alw21I GWGCWC 3 cut(s) 117, 358, 1088
Alw26I GTCTC 3 cut(s) 208, 1481, 1727
AlwNI CAGNNNCTG 2 cut(s) 727, 1100
Ama87I CYCGRG 1 cut(s) 1286
AoxI GGCC 2 cut(s) 248, 1402
ApeKI GCWGC 4 cut(s) 621, 1438, 1727, 1764
ApoI RAATTY 7 cut(s) 284, 296, 464, 636, 1012, 1539, 1633
AsiGI ACCGGT 1 cut(s) 275
Asp700I GAANNNNTTC 1 cut(s) 1012
AspA2I CCTAGG 1 cut(s) 887
AspLEI GCGC 1 cut(s) 1126
AsuC2I CCSGG 3 cut(s) 786, 1287, 1288
AsuHPI GGTGA 5 cut(s) 290, 800, 965, 1557, 1734
AvaI CYCGRG 1 cut(s) 1286
AvrII CCTAGG 1 cut(s) 887
BamHI GGATCC 2 cut(s) 184, 343
BanI GGYRCC 1 cut(s) 1132
BauI CACGAG 1 cut(s) 762
Bbv12I GWGCWC 3 cut(s) 117, 358, 1088
BbvCI CCTCAGC 1 cut(s) 1941
BbvI GCAGC 4 cut(s) 633, 1450, 1739, 1776
BccI CCATC 7 cut(s) 196, 650, 838, 863, 944, 1289, 1814
BceAI ACGGC 1 cut(s) 1417
BcnI CCSGG 3 cut(s) 786, 1287, 1288
BcoDI GTCTC 3 cut(s) 208, 1481, 1727
BfaI CTAG 5 cut(s) 141, 201, 888, 1341, 1698
BfmI CTRYAG 1 cut(s) 619
BfoI RGCGCY 1 cut(s) 1127
BglII AGATCT 1 cut(s) 829
BisI GCNGC 4 cut(s) 622, 1439, 1728, 1765
BlnI CCTAGG 1 cut(s) 887
BlpI GCTNAGC 1 cut(s) 1510
BlsI GCNGC 4 cut(s) 623, 1440, 1729, 1766
BmcAI AGTACT 1 cut(s) 205
BmeT110I CYCGRG 1 cut(s) 1286
BmiI GGNNCC 7 cut(s) 186, 244, 345, 1134, 1323, 1782, 1938
BmrI ACTGGG 1 cut(s) 395
BmsI GCATC 4 cut(s) 488, 720, 790, 1224
BmuI ACTGGG 1 cut(s) 395
BpmI CTGGAG 4 cut(s) 204, 1020, 1392, 1760
Bpu10I CCTNAGC 1 cut(s) 1941
Bpu1102I GCTNAGC 1 cut(s) 1510
BpuEI CTTGAG 1 cut(s) 1074
BpuMI CCSGG 3 cut(s) 786, 1287, 1288
Bsa29I ATCGAT 1 cut(s) 1968
BsaAI YACGTR 2 cut(s) 9, 689
BsaBI GATNNNNATC 2 cut(s) 41, 828
BsaI GGTCTC 1 cut(s) 208
BsaJI CCNNGG 8 cut(s) 320, 887, 1222, 1285, 1286, 1628, 1978, 1979
BsaWI WCCGGW 1 cut(s) 275
Bsc4I CCNNNNNNNGG 8 cut(s) 171, 540, 656, 1223, 1400, 1417, 1418, 1883
Bse118I RCCGGY 1 cut(s) 275
Bse1I ACTGG 3 cut(s) 390, 1262, 1860
Bse3DI GCAATG 1 cut(s) 96
Bse8I GATNNNNATC 2 cut(s) 41, 828
BseCI ATCGAT 1 cut(s) 1968
BseDI CCNNGG 8 cut(s) 320, 887, 1222, 1285, 1286, 1628, 1978, 1979
BseGI GGATG 6 cut(s) 42, 161, 715, 853, 955, 1258
BseJI GATNNNNATC 2 cut(s) 41, 828
BseLI CCNNNNNNNGG 8 cut(s) 171, 540, 656, 1223, 1400, 1417, 1418, 1883
BseMI GCAATG 1 cut(s) 96
BseMII CTCAG 9 cut(s) 609, 941, 951, 1075, 1524, 1524, 1884, 1950, 1955
BseNI ACTGG 3 cut(s) 390, 1262, 1860
BseRI GAGGAG 1 cut(s) 1929
BseXI GCAGC 4 cut(s) 633, 1450, 1739, 1776
BshFI GGCC 2 cut(s) 250, 1404
BshNI GGYRCC 1 cut(s) 1132
BshTI ACCGGT 1 cut(s) 275
BshVI ATCGAT 1 cut(s) 1968
BsiHKAI GWGCWC 3 cut(s) 117, 358, 1088
BsiHKCI CYCGRG 1 cut(s) 1286
BsiSI CCGG 3 cut(s) 276, 786, 1287
BslFI GGGAC 2 cut(s) 1268, 1961
BslI CCNNNNNNNGG 8 cut(s) 171, 540, 656, 1223, 1400, 1417, 1418, 1883
BsmAI GTCTC 3 cut(s) 208, 1481, 1727
BsmFI GGGAC 2 cut(s) 1268, 1961
BsnI GGCC 2 cut(s) 250, 1404
Bso31I GGTCTC 1 cut(s) 208
BsoBI CYCGRG 1 cut(s) 1286
Bsp1286I GDGCHC 3 cut(s) 117, 358, 1088
Bsp1720I GCTNAGC 1 cut(s) 1510
BspACI CCGC 1 cut(s) 1963
BspANI GGCC 2 cut(s) 250, 1404
BspCNI CTCAG 9 cut(s) 608, 940, 952, 1074, 1523, 1525, 1885, 1951, 1954
BspDI ATCGAT 1 cut(s) 1968
BspHI TCATGA 1 cut(s) 300
BspLI GGNNCC 7 cut(s) 186, 244, 345, 1134, 1323, 1782, 1938
BspMAI CTGCAG 1 cut(s) 623
BspQI GCTCTTC 1 cut(s) 1428
BspT107I GGYRCC 1 cut(s) 1132
BspTNI GGTCTC 1 cut(s) 208
BsrBI CCGCTC 1 cut(s) 1963
BsrDI GCAATG 1 cut(s) 96
BsrFI RCCGGY 1 cut(s) 275
BsrI ACTGG 3 cut(s) 390, 1262, 1860
BssAI RCCGGY 1 cut(s) 275
BssECI CCNNGG 8 cut(s) 320, 887, 1222, 1285, 1286, 1628, 1978, 1979
BssSI CACGAG 1 cut(s) 762
BssT1I CCWWGG 2 cut(s) 320, 887
Bst2BI CACGAG 1 cut(s) 762
Bst4CI ACNGT 5 cut(s) 699, 1108, 1654, 1676, 1869
Bst6I CTCTTC 3 cut(s) 1047, 1428, 1580
BstAPI GCANNNNNTGC 2 cut(s) 485, 1234
BstBAI YACGTR 2 cut(s) 9, 689
BstC8I GCNNGC 2 cut(s) 1117, 1454
BstENI CCTNNNNNAGG 1 cut(s) 538
BstF5I GGATG 6 cut(s) 42, 161, 715, 853, 955, 1258
BstH2I RGCGCY 1 cut(s) 1127
BstHHI GCGC 1 cut(s) 1126
BstMAI GTCTC 3 cut(s) 208, 1481, 1727
BstMWI GCNNNNNNNGC 6 cut(s) 242, 485, 1121, 1130, 1234, 1724
BstNSI RCATGY 1 cut(s) 1456
BstSFI CTRYAG 1 cut(s) 619
BstV1I GCAGC 4 cut(s) 633, 1450, 1739, 1776
BstX2I RGATCY 6 cut(s) 184, 343, 370, 724, 793, 829
BstYI RGATCY 6 cut(s) 184, 343, 370, 724, 793, 829
Bsu15I ATCGAT 1 cut(s) 1968
BsuRI GGCC 2 cut(s) 250, 1404
BsuTUI ATCGAT 1 cut(s) 1968
BtsCI GGATG 6 cut(s) 42, 161, 715, 853, 955, 1258
BtsI GCAGTG 1 cut(s) 1101
BtsIMutI CAGTG 5 cut(s) 603, 1101, 1269, 1650, 1867
Cac8I GCNNGC 2 cut(s) 1117, 1454
CaiI CAGNNNCTG 2 cut(s) 727, 1100
CciI TCATGA 1 cut(s) 300
CfoI GCGC 1 cut(s) 1126
Cfr10I RCCGGY 1 cut(s) 275
Cfr9I CCCGGG 1 cut(s) 1286
ClaI ATCGAT 1 cut(s) 1968
Csp6I GTAC 3 cut(s) 175, 204, 518
CspAI ACCGGT 1 cut(s) 275
CviQI GTAC 3 cut(s) 175, 204, 518
EaeI YGGCCR 1 cut(s) 1402
Eam1104I CTCTTC 3 cut(s) 1047, 1428, 1580
EarI CTCTTC 3 cut(s) 1047, 1428, 1580
Eco130I CCWWGG 2 cut(s) 320, 887
Eco147I AGGCCT 1 cut(s) 250
Eco31I GGTCTC 1 cut(s) 208
Eco57I CTGAAG 2 cut(s) 561, 1827
Eco88I CYCGRG 1 cut(s) 1286
EcoNI CCTNNNNNAGG 1 cut(s) 538
EcoRI GAATTC 2 cut(s) 296, 1012
EcoT14I CCWWGG 2 cut(s) 320, 887
EcoT22I ATGCAT 1 cut(s) 1239
ErhI CCWWGG 2 cut(s) 320, 887
FalI AAGNNNNNCTT 2 cut(s) 491, 523
FaqI GGGAC 2 cut(s) 1268, 1961
FblI GTMKAC 1 cut(s) 1871
Fnu4HI GCNGC 4 cut(s) 622, 1439, 1728, 1765
FokI GGATG 6 cut(s) 49, 148, 722, 860, 962, 1245
Fsp4HI GCNGC 4 cut(s) 622, 1439, 1728, 1765
FspBI CTAG 5 cut(s) 141, 201, 888, 1341, 1698
GlaI GCGC 1 cut(s) 1125
GluI GCNGC 4 cut(s) 622, 1439, 1728, 1765
GsuI CTGGAG 4 cut(s) 204, 1020, 1392, 1760
HaeII RGCGCY 1 cut(s) 1127
HaeIII GGCC 2 cut(s) 250, 1404
HapII CCGG 3 cut(s) 276, 786, 1287
HhaI GCGC 1 cut(s) 1126
Hin6I GCGC 1 cut(s) 1124
HinP1I GCGC 1 cut(s) 1124
HincII GTYRAC 2 cut(s) 744, 1269
HindII GTYRAC 2 cut(s) 744, 1269
HinfI GANTC 8 cut(s) 512, 923, 956, 964, 1202, 1337, 1385, 1901
HpaII CCGG 3 cut(s) 276, 786, 1287
HphI GGTGA 5 cut(s) 290, 800, 965, 1557, 1734
Hpy166II GTNNAC 6 cut(s) 316, 744, 1269, 1529, 1865, 1872
Hpy188III TCNNGA 8 cut(s) 221, 293, 301, 579, 797, 1005, 1173, 1758
Hpy8I GTNNAC 6 cut(s) 316, 744, 1269, 1529, 1865, 1872
Hpy99I CGWCG 1 cut(s) 152
HpyAV CCTTC 5 cut(s) 654, 1137, 1323, 1549, 1674
HpyCH4III ACNGT 5 cut(s) 699, 1108, 1654, 1676, 1869
HpyCH4IV ACGT 4 cut(s) 8, 117, 688, 1071
HpyCH4V TGCA 9 cut(s) 89, 488, 621, 733, 767, 803, 835, 1076, 1237
HpyF10VI GCNNNNNNNGC 6 cut(s) 242, 485, 1121, 1130, 1234, 1724
HpySE526I ACGT 4 cut(s) 8, 117, 688, 1071
HspAI GCGC 1 cut(s) 1124
LguI GCTCTTC 1 cut(s) 1428
LmnI GCTCC 4 cut(s) 242, 361, 1779, 1942
Lsp1109I GCAGC 4 cut(s) 633, 1450, 1739, 1776
LweI GCATC 4 cut(s) 488, 720, 790, 1224
MaeI CTAG 5 cut(s) 141, 201, 888, 1341, 1698
MaeII ACGT 4 cut(s) 8, 117, 688, 1071
MaeIII GTNAC 3 cut(s) 49, 429, 503
MbiI CCGCTC 1 cut(s) 1963
MflI RGATCY 6 cut(s) 184, 343, 370, 724, 793, 829
MhlI GDGCHC 3 cut(s) 117, 358, 1088
MmeI TCCRAC 2 cut(s) 220, 655
Mph1103I ATGCAT 1 cut(s) 1239
MroXI GAANNNNTTC 1 cut(s) 1012
MseI TTAA 1 cut(s) 701
MslI CAYNNNNRTG 1 cut(s) 1216
MspI CCGG 3 cut(s) 276, 786, 1287
MwoI GCNNNNNNNGC 6 cut(s) 242, 485, 1121, 1130, 1234, 1724
NciI CCSGG 3 cut(s) 786, 1287, 1288
NlaIV GGNNCC 7 cut(s) 186, 244, 345, 1134, 1323, 1782, 1938
NmuCI GTSAC 1 cut(s) 49
NsiI ATGCAT 1 cut(s) 1239
NspI RCATGY 1 cut(s) 1456
PaeI GCATGC 1 cut(s) 1456
PagI TCATGA 1 cut(s) 300
PasI CCCWGGG 1 cut(s) 1979
PceI AGGCCT 1 cut(s) 250
PciSI GCTCTTC 1 cut(s) 1428
PdmI GAANNNNTTC 1 cut(s) 1012
PfeI GAWTC 8 cut(s) 512, 923, 956, 964, 1202, 1337, 1385, 1901
PfoI TCCNGGA 4 cut(s) 180, 459, 1776, 1876
PinAI ACCGGT 1 cut(s) 275
PkrI GCNGC 4 cut(s) 623, 1440, 1729, 1766
Ppu21I YACGTR 2 cut(s) 9, 689
Psp1406I AACGTT 1 cut(s) 1071
PspN4I GGNNCC 7 cut(s) 186, 244, 345, 1134, 1323, 1782, 1938
PstI CTGCAG 1 cut(s) 623
PstNI CAGNNNCTG 2 cut(s) 727, 1100
PsuI RGATCY 6 cut(s) 184, 343, 370, 724, 793, 829
RsaI GTAC 3 cut(s) 176, 205, 519
RsaNI GTAC 3 cut(s) 175, 204, 518
RseI CAYNNNNRTG 1 cut(s) 1216
SapI GCTCTTC 1 cut(s) 1428
SaqAI TTAA 1 cut(s) 701
SatI GCNGC 4 cut(s) 622, 1439, 1728, 1765
ScaI AGTACT 1 cut(s) 205
SduI GDGCHC 3 cut(s) 117, 358, 1088
SfaNI GCATC 4 cut(s) 488, 720, 790, 1224
SfcI CTRYAG 1 cut(s) 619
SmaI CCCGGG 1 cut(s) 1288
SmiMI CAYNNNNRTG 1 cut(s) 1216
SmlI CTYRAG 1 cut(s) 1089
SmoI CTYRAG 1 cut(s) 1089
SphI GCATGC 1 cut(s) 1456
SseBI AGGCCT 1 cut(s) 250
SsiI CCGC 1 cut(s) 1963
SspI AATATT 1 cut(s) 1381
SspMI CTAG 5 cut(s) 141, 201, 888, 1341, 1698
StuI AGGCCT 1 cut(s) 250
StyI CCWWGG 2 cut(s) 320, 887
TaaI ACNGT 5 cut(s) 699, 1108, 1654, 1676, 1869
TaiI ACGT 4 cut(s) 11, 120, 691, 1074
TaqI TCGA 3 cut(s) 556, 1786, 1968
TatI WGTACW 1 cut(s) 203
TfiI GAWTC 8 cut(s) 512, 923, 956, 964, 1202, 1337, 1385, 1901
Tru1I TTAA 1 cut(s) 701
Tru9I TTAA 1 cut(s) 701
TscAI CASTG 5 cut(s) 603, 1101, 1269, 1657, 1867
TseFI GTSAC 1 cut(s) 49
TseI GCWGC 4 cut(s) 621, 1438, 1727, 1764
Tsp45I GTSAC 1 cut(s) 49
TspGWI ACGGA 1 cut(s) 165
TspMI CCCGGG 1 cut(s) 1286
TspRI CASTG 5 cut(s) 603, 1101, 1269, 1657, 1867
XagI CCTNNNNNAGG 1 cut(s) 538
XapI RAATTY 7 cut(s) 284, 296, 464, 636, 1012, 1539, 1633
XceI RCATGY 1 cut(s) 1456
XcmI CCANNNNNNNNNTGG 1 cut(s) 1859
XmaI CCCGGG 1 cut(s) 1286
XmaJI CCTAGG 1 cut(s) 887
XmiI GTMKAC 1 cut(s) 1871
XmnI GAANNNNTTC 1 cut(s) 1012
XspI CTAG 5 cut(s) 141, 201, 888, 1341, 1698
ZrmI AGTACT 1 cut(s) 205
Zsp2I ATGCAT 1 cut(s) 1239
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.