Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr10

Orthogroup ID

OG0005652

Physical Location & Seq

Genomic Coordinates Forward (+)

29417489 .. 29424411

Gene Structure 6923 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

MD10G1195900.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2307 bp

ATGGGCCTGCAATTCCAATTGCAGATGAGTTGGCAGCCGAGTCTACTGAGTCAGAAGCGCAAGAATGGCCTGCCGCCTCTAGGGTTACGCAACCTCGGCAACTCTTGCTACCTCAACAGCGTCCTCCAGTGCCTCACCTACACCCCTCCTCTCGCCAATTTCTGCCTCCGCAAACTCCACACCTCTCTCTGTGACTCTTCGGATGCGGAGCGGAAGCGCGACTGTCCCTTTTGCATTCTGGAGAAGCGGATAGTGCGCTCGCTGAGTCTGGATCTCAGTCTGGATGCGCCGCAGAAGATACAGAGCTGTCTTGGGATTTTTGCCGAACACTTTCGGGGCGGTCGACAGGAGGACGCTCATGAGTTCCTGCGCTACGTCATTGATGCTTGCCACAACACCTGCCTTCGGCTCATGAAGCTGCGGCGCAATGCCACTGCCAGCGGAAAGGCTGCCAACGGCAATGTCGCCAACCGCATAGGCAATGGAGAGGCGGCAACATCGACGGTGGTGAAGGAGATATTTGGTGGAGCATTGCAGAGCCAGGTGAAATGTCTATCCTGTGGCACAGAATCCAATAAGGTTGATGAGATAATGGATATTAGTCTTGATGTTTTGCATAGTAATTCGCTTAAAGACGCATTGCAAAGGTTCTTTCAGCCTGAGATTTTGGATGGCCAGAACAAGTACAAATGCGAAAGTTGTAACAAATTGGTCGCAGCACGGAAGCAAATGTCTTTTCTTCAAGCACCCAATGTTCTCGTTATCCAACTTAAGAGATTTGAGGGTATACTTGGCGGGAAGATTGATAAATCCATTTCGTTTGAGGAGGTTTTGGTGCTTTCGGGCTTCATGTGCAAAACAAGCAAGGATCCGCGGCCAGAGTATAAGCTTTTTGGTACTATTGTGCATTCAGGCTTCTCACCAGAATCTGGTCATTATTATGCATATATTAAGGATGCCATAGGTCGGTGGTATTGCTGCAACGATTCATTTGTCTCAATTTCAACCTTGCAAAAGGTCTTATCTGAAAAGGCTTACATCCTTTTCTTCTCTCGTACCAACCAAAGACCAGCATTGGCTAGCATTGCTTCCAATGGGGTGAGCTCTCATGTTTGTAATGGTCGTGAAACGTCTAAAATCCCGAAGTCCGCTCTTCCACCAAAAGGAATGCAAACGAAACCTTATGAGCAGTCTTCTTGGAAGGATGTCTCAGCCGTATCTAAAGTTGATAAAGTGCCTTATTGCCCACGGATGAAGTTTGATCTCAGTGGAAACTCTAGCTCCAAAAGAGCTCCCACAACTGATAATGGAAAAACTGTTTACAAGAATCAATCCTTAGGATCAAACGGGGAAAGATCTACTTCAACTGCTAATGGCAAAACTGTTTGCAAGAATCAATCATTAGGATCAAACGGGGAAAGTTCTACTTCGACTGCTAATGGCAAAAGTGTTAACAAGAGTCAATTCTTAGGTTCAAATGAGGAAAGAGTTCCGGCAACTGATAATGGAAAAATTGCTTACAGAAATGAATCCTTTGGATCAAATGGGGAAGGAGCTCCTGCAAATGATAATGGAAAAATTGCTGACAAGAATCAATCCCTACGATCAAATGGTGAAAGGGCACCTGGAACTGATAATGGAAAAATTATTTACAAGAATCAATCCATAGGATTAAATGTGGATTCAAAGGATCCTGTTATAGTTGACGGTAAAAGTAGCAAAGCATCTTTGTTATCACGGGAAAATGGTGGTGCTGGTTCCATAAAAACTGAACCCTGTGAAGGTATTGATACAAGAGACAGGTTGGCAGCTGGGAAAGTGCCTAACAGTAGCCAAGCATCTTTGTTATCCCGAGAAAATGGTGGAACTGGTTCAGTAAAAACTGAACCCTGTGAAGGTATAGGTACAAGAGACAGGTTGACAGCTAGTATAGTGCCTGATCCGAGTGAATTGGAAAAAAGTGGTCTTCATGGCCGCTCTAAGGTCTCTAGGTCGAAAAGAAAATCTCTGGATTCGTGCATTCTGTTTGCACCTGATGCTCAATCCCGGGCAGAGGTTGAAGGACTAAAGGAAATTCTCGAGAAAGAGGCTTCTTCTGTTTTGCGATCATGTGGCTGGTTGGATGAAGTTTACAGTTTTATGCGTTCAAGGAAAAGATTATGTTCAGAGCAAGTTGAAACCACTCCAAACTACGATGAAGTGAAGAAGATGTTGATCATGGATGCCAAATCAAGTTTTCTTTCACAGATACCCTCGTCTTTAAGAGGGGACTTAGTCAAGCGCATTCAATCATTTAGCAAAAAATAG

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005829 GO:0005840 GO:0022626 GO:0032991 GO:0043226 GO:0043228 GO:0043229 GO:0043232 GO:0044424 GO:0044444 GO:0044445 GO:0044464 GO:1990904

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

UCH

Protein Analysis

769

Amino Acids

83.93

Weight (kDa)

9.26

Isoelectric Point (pI)

48.31

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
UCH	PF00443	28 - 350	6.4e-56	Ubiquitin carboxyl-terminal hydrolase
UCH_1	PF13423	28 - 332	3.5e-17	Ubiquitin carboxyl-terminal hydrolase

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0005652)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT3G14400
fragaria_vesca	FvH4_3g16490 FvH4_3g16490 FvH4_3g16490 FvH4_3g16490
malus_domestica	MD05G1209600.v1.1 MD10G1195900.v1.1
prunus_persica	Prupe.4G147900_v2.0.a1
pyrus_communis	pycom05g19560 pycom10g16950
rosa_chinensis	RchiOBHm_Chr5g0027541
rosa_laevigata	RLG00000033025
rosa_multiflora	Rmu_co8326735.1_g000001 Rmu_co8467017.1_g000001 Rmu_sc0004493.1_g000008 Rmu_sc0007802.1_g000039 Rmu_sc0011056.1_g000009
rosa_roxburghii	Rroxscaffold_1G00052000 Rroxscaffold_1G00052020
rosa_rugosa	Rorug05G0099400 Rorug05G0099500 Rorug05G0099600
rosa_samantha	Rh5AG193700 Rh5BG191700 Rh5CG211900 Rh5DG193400 Rh5DG193600
rosa_wichuraiana	Rw5G017660

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AarI	CACCTGC	1 cut(s)	407
Acc36I	ACCTGC	1 cut(s)	407
AccB1I	GGYRCC	1 cut(s)	1621
AccB7I	CCANNNNNTGG	1 cut(s)	929
AccBSI	CCGCTC	3 cut(s)	211, 1151, 1977
AccI	GTMKAC	3 cut(s)	43, 343, 787
AccII	CGCG	2 cut(s)	219, 874
AclWI	GGATC	9 cut(s)	279, 863, 876, 1348, 1414, 1546, 1685, 1698, 1934
AcoI	YGGCCR	3 cut(s)	673, 875, 1972
AcsI	RAATTY	1 cut(s)	2073
AfaI	GTAC	4 cut(s)	686, 898, 1057, 1906
AfiI	CCNNNNNNNGG	9 cut(s)	80, 405, 929, 966, 1163, 1781, 1895, 1981, 2053
AflII	CTTAAG	1 cut(s)	770
AgsI	TTSAA	9 cut(s)	743, 1005, 1365, 1476, 1686, 2060, 2148, 2177, 2288
AjnI	CCWGG	2 cut(s)	540, 1624
AluBI	AGCT	9 cut(s)	306, 418, 889, 1104, 1281, 1292, 1556, 1811, 1925
AluI	AGCT	9 cut(s)	306, 418, 889, 1104, 1281, 1292, 1556, 1811, 1925
Alw21I	GWGCWC	3 cut(s)	1106, 1294, 1558
Alw26I	GTCTC	5 cut(s)	1000, 1213, 1791, 1905, 1990
AlwI	GGATC	9 cut(s)	279, 863, 876, 1348, 1414, 1546, 1685, 1698, 1934
AlwNI	CAGNNNCTG	1 cut(s)	929
Ama87I	CYCGRG	3 cut(s)	1851, 2046, 2078
AoxI	GGCC	5 cut(s)	4, 67, 673, 875, 1972
ApeKI	GCWGC	6 cut(s)	34, 418, 449, 716, 978, 1808
ApoI	RAATTY	1 cut(s)	2073
Asp700I	GAANNNNTTC	3 cut(s)	330, 1488, 1870
AspLEI	GCGC	7 cut(s)	60, 219, 258, 289, 372, 426, 2283
AspS9I	GGNCC	1 cut(s)	4
AsuC2I	CCSGG	2 cut(s)	2047, 2048
AsuHPI	GGTGA	6 cut(s)	127, 520, 556, 912, 1111, 1625
AsuNHI	GCTAGC	1 cut(s)	1079
AvaI	CYCGRG	3 cut(s)	1851, 2046, 2078
AxyI	CCTNAGG	1 cut(s)	1336
BaeGI	GKGCMC	1 cut(s)	1624
BaeI	ACNNNNGTAYC	4 cut(s)	888, 921, 2240, 2273
BalI	TGGCCA	1 cut(s)	675
BamHI	GGATCC	2 cut(s)	868, 1690
BanI	GGYRCC	1 cut(s)	1621
BanII	GRGCYC	3 cut(s)	1106, 1294, 1558
BbsI	GAAGAC	2 cut(s)	1185, 1958
Bbv12I	GWGCWC	3 cut(s)	1106, 1294, 1558
BbvI	GCAGC	6 cut(s)	46, 405, 436, 728, 965, 1820
BccI	CCATC	1 cut(s)	665
BceAI	ACGGC	2 cut(s)	472, 1199
BciT130I	CCWGG	2 cut(s)	542, 1626
BclI	TGATCA	1 cut(s)	2214
BcnI	CCSGG	2 cut(s)	2047, 2048
BcoDI	GTCTC	5 cut(s)	1000, 1213, 1791, 1905, 1990
BfaI	CTAG	5 cut(s)	80, 1080, 1278, 1926, 1989
BfrI	CTTAAG	1 cut(s)	770
BfuAI	ACCTGC	1 cut(s)	407
BglII	AGATCT	1 cut(s)	1355
Bme1390I	CCNGG	4 cut(s)	542, 1626, 2047, 2048
BmeT110I	CYCGRG	3 cut(s)	1851, 2046, 2078
BmgT120I	GGNCC	1 cut(s)	4
BmiI	GGNNCC	4 cut(s)	870, 1623, 1692, 1759
BmrFI	CCNGG	4 cut(s)	542, 1626, 2047, 2048
BmsI	GCATC	8 cut(s)	193, 274, 373, 946, 1733, 1847, 2026, 2212
BmtI	GCTAGC	1 cut(s)	1083
BpiI	GAAGAC	2 cut(s)	1185, 1958
BpmI	CTGGAG	2 cut(s)	110, 260
BpuMI	CCSGG	2 cut(s)	2047, 2048
BsaBI	GATNNNNATC	1 cut(s)	2213
BsaI	GGTCTC	1 cut(s)	1990
BsaJI	CCNNGG	4 cut(s)	94, 872, 1247, 2046
BsaXI	ACNNNNNCTCC	2 cut(s)	1265, 1295
Bsc4I	CCNNNNNNNGG	9 cut(s)	80, 405, 929, 966, 1163, 1781, 1895, 1981, 2053
Bse1I	ACTGG	2 cut(s)	127, 1873
Bse21I	CCTNAGG	1 cut(s)	1336
Bse3DI	GCAATG	6 cut(s)	433, 466, 487, 530, 638, 1083
Bse8I	GATNNNNATC	1 cut(s)	2213
BseBI	CCWGG	2 cut(s)	542, 1626
BseDI	CCNNGG	4 cut(s)	94, 872, 1247, 2046
BseGI	GGATG	9 cut(s)	208, 289, 676, 961, 1038, 1210, 1257, 2128, 2227
BseJI	GATNNNNATC	1 cut(s)	2213
BseLI	CCNNNNNNNGG	9 cut(s)	80, 405, 929, 966, 1163, 1781, 1895, 1981, 2053
BseMI	GCAATG	6 cut(s)	433, 466, 487, 530, 638, 1083
BseMII	CTCAG	6 cut(s)	38, 254, 289, 651, 1224, 1279
BseNI	ACTGG	2 cut(s)	127, 1873
BseRI	GAGGAG	2 cut(s)	138, 839
BseSI	GKGCMC	1 cut(s)	1624
BseXI	GCAGC	6 cut(s)	46, 405, 436, 728, 965, 1820
BseYI	CCCAGC	1 cut(s)	1811
Bsh1236I	CGCG	2 cut(s)	219, 874
Bsh1285I	CGRYCG	1 cut(s)	343
BshFI	GGCC	5 cut(s)	6, 69, 675, 877, 1974
BshNI	GGYRCC	1 cut(s)	1621
BsiEI	CGRYCG	1 cut(s)	343
BsiHKAI	GWGCWC	3 cut(s)	1106, 1294, 1558
BsiHKCI	CYCGRG	3 cut(s)	1851, 2046, 2078
BsiSI	CCGG	2 cut(s)	1493, 2047
BslFI	GGGAC	2 cut(s)	210, 2282
BslI	CCNNNNNNNGG	9 cut(s)	80, 405, 929, 966, 1163, 1781, 1895, 1981, 2053
BsmAI	GTCTC	5 cut(s)	1000, 1213, 1791, 1905, 1990
BsmFI	GGGAC	2 cut(s)	210, 2282
BsmI	GAATGC	5 cut(s)	234, 907, 1173, 2019, 2283
BsnI	GGCC	5 cut(s)	6, 69, 675, 877, 1974
Bso31I	GGTCTC	1 cut(s)	1990
BsoBI	CYCGRG	3 cut(s)	1851, 2046, 2078
Bsp1286I	GDGCHC	4 cut(s)	1106, 1294, 1558, 1624
BspANI	GGCC	5 cut(s)	6, 69, 675, 877, 1974
BspCNI	CTCAG	6 cut(s)	39, 255, 288, 652, 1223, 1278
BspFNI	CGCG	2 cut(s)	219, 874
BspHI	TCATGA	2 cut(s)	358, 411
BspLI	GGNNCC	4 cut(s)	870, 1623, 1692, 1759
BspMI	ACCTGC	1 cut(s)	407
BspOI	GCTAGC	1 cut(s)	1083
BspPI	GGATC	9 cut(s)	279, 863, 876, 1348, 1414, 1546, 1685, 1698, 1934
BspQI	GCTCTTC	1 cut(s)	1158
BspT107I	GGYRCC	1 cut(s)	1621
BspTI	CTTAAG	1 cut(s)	770
BspTNI	GGTCTC	1 cut(s)	1990
BsrBI	CCGCTC	3 cut(s)	211, 1151, 1977
BsrDI	GCAATG	6 cut(s)	433, 466, 487, 530, 638, 1083
BsrI	ACTGG	2 cut(s)	127, 1873
BssECI	CCNNGG	4 cut(s)	94, 872, 1247, 2046
BssNAI	GTATAC	1 cut(s)	788
Bst1107I	GTATAC	1 cut(s)	788
Bst2UI	CCWGG	2 cut(s)	542, 1626
Bst4CI	ACNGT	7 cut(s)	224, 505, 1318, 1384, 1709, 1829, 2135
Bst6I	CTCTTC	2 cut(s)	202, 1158
BstAFI	CTTAAG	1 cut(s)	770
BstAPI	GCANNNNNTGC	2 cut(s)	105, 2036
BstC8I	GCNNGC	6 cut(s)	8, 71, 260, 388, 439, 1081
BstDSI	CCRYGG	2 cut(s)	872, 1247
BstF5I	GGATG	9 cut(s)	208, 289, 676, 961, 1038, 1210, 1257, 2128, 2227
BstFNI	CGCG	2 cut(s)	219, 874
BstHHI	GCGC	7 cut(s)	60, 219, 258, 289, 372, 426, 2283
BstMAI	GTCTC	5 cut(s)	1000, 1213, 1791, 1905, 1990
BstMCI	CGRYCG	1 cut(s)	343
BstMWI	GCNNNNNNNGC	9 cut(s)	66, 96, 105, 253, 415, 852, 861, 1085, 2036
BstNI	CCWGG	2 cut(s)	542, 1626
BstSCI	CCNGG	4 cut(s)	540, 1624, 2045, 2046
BstSLI	GKGCMC	1 cut(s)	1624
BstUI	CGCG	2 cut(s)	219, 874
BstV1I	GCAGC	6 cut(s)	46, 405, 436, 728, 965, 1820
BstV2I	GAAGAC	2 cut(s)	1185, 1958
BstX2I	RGATCY	4 cut(s)	271, 868, 1355, 1690
BstYI	RGATCY	4 cut(s)	271, 868, 1355, 1690
BstZ17I	GTATAC	1 cut(s)	788
Bsu36I	CCTNAGG	1 cut(s)	1336
BsuRI	GGCC	5 cut(s)	6, 69, 675, 877, 1974
BtgI	CCRYGG	2 cut(s)	872, 1247
BtsCI	GGATG	9 cut(s)	208, 289, 676, 961, 1038, 1210, 1257, 2128, 2227
BtsI	GCAGTG	1 cut(s)	432
BtsIMutI	CAGTG	3 cut(s)	134, 432, 1273
BveI	ACCTGC	1 cut(s)	407
Cac8I	GCNNGC	6 cut(s)	8, 71, 260, 388, 439, 1081
CaiI	CAGNNNCTG	1 cut(s)	929
CciI	TCATGA	2 cut(s)	358, 411
CfoI	GCGC	7 cut(s)	60, 219, 258, 289, 372, 426, 2283
Cfr13I	GGNCC	1 cut(s)	4
Cfr42I	CCGCGG	1 cut(s)	875
Cfr9I	CCCGGG	1 cut(s)	2046
CseI	GACGC	3 cut(s)	109, 362, 644
Csp6I	GTAC	4 cut(s)	685, 897, 1056, 1905
CviAII	CATG	7 cut(s)	359, 412, 850, 1109, 1970, 2109, 2218
CviQI	GTAC	4 cut(s)	685, 897, 1056, 1905
EaeI	YGGCCR	3 cut(s)	673, 875, 1972
Eam1104I	CTCTTC	2 cut(s)	202, 1158
EarI	CTCTTC	2 cut(s)	202, 1158
Ecl136II	GAGCTC	3 cut(s)	1104, 1292, 1556
Eco24I	GRGCYC	3 cut(s)	1106, 1294, 1558
Eco31I	GGTCTC	1 cut(s)	1990
Eco53kI	GAGCTC	3 cut(s)	1104, 1292, 1556
Eco81I	CCTNAGG	1 cut(s)	1336
Eco88I	CYCGRG	3 cut(s)	1851, 2046, 2078
EcoICRI	GAGCTC	3 cut(s)	1104, 1292, 1556
EcoRII	CCWGG	2 cut(s)	540, 1624
EcoT22I	ATGCAT	1 cut(s)	946
EcoT38I	GRGCYC	3 cut(s)	1106, 1294, 1558
FaeI	CATG	7 cut(s)	362, 415, 853, 1112, 1973, 2112, 2221
FalI	AAGNNNNNCTT	4 cut(s)	1345, 1377, 1411, 1443
FaqI	GGGAC	2 cut(s)	210, 2282
FatI	CATG	7 cut(s)	358, 411, 849, 1108, 1969, 2108, 2217
FauI	CCCGC	1 cut(s)	788
FbaI	TGATCA	1 cut(s)	2214
FblI	GTMKAC	3 cut(s)	43, 343, 787
FokI	GGATG	9 cut(s)	215, 296, 683, 968, 1025, 1217, 1264, 2135, 2234
FriOI	GRGCYC	3 cut(s)	1106, 1294, 1558
FspBI	CTAG	5 cut(s)	80, 1080, 1278, 1926, 1989
GlaI	GCGC	7 cut(s)	59, 218, 257, 288, 371, 425, 2282
GsaI	CCCAGC	1 cut(s)	1815
GsuI	CTGGAG	2 cut(s)	110, 260
HaeIII	GGCC	5 cut(s)	6, 69, 675, 877, 1974
HapII	CCGG	2 cut(s)	1493, 2047
HgaI	GACGC	3 cut(s)	109, 362, 644
HhaI	GCGC	7 cut(s)	60, 219, 258, 289, 372, 426, 2283
Hin1II	CATG	7 cut(s)	362, 415, 853, 1112, 1973, 2112, 2221
Hin6I	GCGC	7 cut(s)	58, 217, 256, 287, 370, 424, 2281
HinP1I	GCGC	7 cut(s)	58, 217, 256, 287, 370, 424, 2281
HincII	GTYRAC	4 cut(s)	344, 1453, 1705, 1920
HindII	GTYRAC	4 cut(s)	344, 1453, 1705, 1920
HindIII	AAGCTT	1 cut(s)	887
HpaI	GTTAAC	1 cut(s)	1453
HpaII	CCGG	2 cut(s)	1493, 2047
HphI	GGTGA	6 cut(s)	127, 520, 556, 912, 1111, 1625
Hpy166II	GTNNAC	8 cut(s)	44, 344, 788, 1321, 1453, 1705, 1920, 2131
Hpy188I	TCNGA	5 cut(s)	54, 202, 1027, 1944, 2167
Hpy8I	GTNNAC	8 cut(s)	44, 344, 788, 1321, 1453, 1705, 1920, 2131
Hpy99I	CGWCG	1 cut(s)	505
HpyAV	CCTTC	7 cut(s)	413, 505, 1195, 1544, 1775, 1889, 2054
HpyCH4III	ACNGT	7 cut(s)	224, 505, 1318, 1384, 1709, 1829, 2135
HpyCH4IV	ACGT	2 cut(s)	375, 1130
HpyF10VI	GCNNNNNNNGC	9 cut(s)	66, 96, 105, 253, 415, 852, 861, 1085, 2036
HpySE526I	ACGT	2 cut(s)	375, 1130
Hsp92II	CATG	7 cut(s)	362, 415, 853, 1112, 1973, 2112, 2221
HspAI	GCGC	7 cut(s)	58, 217, 256, 287, 370, 424, 2281
Ksp22I	TGATCA	1 cut(s)	2214
KspAI	GTTAAC	1 cut(s)	1453
KspI	CCGCGG	1 cut(s)	875
LguI	GCTCTTC	1 cut(s)	1158
LmnI	GCTCC	6 cut(s)	208, 527, 1286, 1297, 1553, 1561
Lsp1109I	GCAGC	6 cut(s)	46, 405, 436, 728, 965, 1820
LweI	GCATC	8 cut(s)	193, 274, 373, 946, 1733, 1847, 2026, 2212
MaeI	CTAG	5 cut(s)	80, 1080, 1278, 1926, 1989
MaeII	ACGT	2 cut(s)	375, 1130
MaeIII	GTNAC	3 cut(s)	84, 191, 701
MbiI	CCGCTC	3 cut(s)	211, 1151, 1977
MfeI	CAATTG	1 cut(s)	17
MflI	RGATCY	4 cut(s)	271, 868, 1355, 1690
MhlI	GDGCHC	4 cut(s)	1106, 1294, 1558, 1624
MlsI	TGGCCA	1 cut(s)	675
MluNI	TGGCCA	1 cut(s)	675
MlyI	GAGTC	5 cut(s)	49, 58, 188, 274, 1468
MmeI	TCCRAC	2 cut(s)	790, 2100
Mox20I	TGGCCA	1 cut(s)	675
Mph1103I	ATGCAT	1 cut(s)	946
MroXI	GAANNNNTTC	3 cut(s)	330, 1488, 1870
MscI	TGGCCA	1 cut(s)	675
MseI	TTAA	6 cut(s)	630, 771, 951, 1452, 1673, 2261
MslI	CAYNNNNRTG	1 cut(s)	939
Msp20I	TGGCCA	1 cut(s)	675
MspA1I	CMGCKG	3 cut(s)	441, 874, 1811
MspCI	CTTAAG	1 cut(s)	770
MspI	CCGG	2 cut(s)	1493, 2047
MspR9I	CCNGG	4 cut(s)	542, 1626, 2047, 2048
MunI	CAATTG	1 cut(s)	17
Mva1269I	GAATGC	5 cut(s)	234, 907, 1173, 2019, 2283
MvaI	CCWGG	2 cut(s)	542, 1626
MvnI	CGCG	2 cut(s)	219, 874
MwoI	GCNNNNNNNGC	9 cut(s)	66, 96, 105, 253, 415, 852, 861, 1085, 2036
NciI	CCSGG	2 cut(s)	2047, 2048
NheI	GCTAGC	1 cut(s)	1079
NlaIII	CATG	7 cut(s)	362, 415, 853, 1112, 1973, 2112, 2221
NlaIV	GGNNCC	4 cut(s)	870, 1623, 1692, 1759
NmeAIII	GCCGAG	2 cut(s)	63, 75
NmuCI	GTSAC	1 cut(s)	191
NsiI	ATGCAT	1 cut(s)	946
PaeR7I	CTCGAG	1 cut(s)	2078
PagI	TCATGA	2 cut(s)	358, 411
PaqCI	CACCTGC	1 cut(s)	407
PciSI	GCTCTTC	1 cut(s)	1158
PcsI	WCGNNNNNNNCGW	1 cut(s)	340
PctI	GAATGC	5 cut(s)	234, 907, 1173, 2019, 2283
PdmI	GAANNNNTTC	3 cut(s)	330, 1488, 1870
PflFI	GACNNNGTC	1 cut(s)	2273
PflMI	CCANNNNNTGG	1 cut(s)	929
PleI	GAGTC	5 cut(s)	48, 57, 188, 273, 1467
PpsI	GAGTC	5 cut(s)	48, 57, 188, 273, 1467
Psp124BI	GAGCTC	3 cut(s)	1106, 1294, 1558
Psp6I	CCWGG	2 cut(s)	540, 1624
PspFI	CCCAGC	1 cut(s)	1811
PspGI	CCWGG	2 cut(s)	540, 1624
PspN4I	GGNNCC	4 cut(s)	870, 1623, 1692, 1759
PspPI	GGNCC	1 cut(s)	4
PstNI	CAGNNNCTG	1 cut(s)	929
PsuI	RGATCY	4 cut(s)	271, 868, 1355, 1690
PsyI	GACNNNGTC	1 cut(s)	2273
PvuII	CAGCTG	1 cut(s)	1811
RsaI	GTAC	4 cut(s)	686, 898, 1057, 1906
RsaNI	GTAC	4 cut(s)	685, 897, 1056, 1905
RseI	CAYNNNNRTG	1 cut(s)	939
SacI	GAGCTC	3 cut(s)	1106, 1294, 1558
SacII	CCGCGG	1 cut(s)	875
SalI	GTCGAC	1 cut(s)	342
SapI	GCTCTTC	1 cut(s)	1158
SaqAI	TTAA	6 cut(s)	630, 771, 951, 1452, 1673, 2261
Sau96I	GGNCC	1 cut(s)	4
SchI	GAGTC	5 cut(s)	49, 58, 188, 274, 1468
ScrFI	CCNGG	4 cut(s)	542, 1626, 2047, 2048
SduI	GDGCHC	4 cut(s)	1106, 1294, 1558, 1624
SfaNI	GCATC	8 cut(s)	193, 274, 373, 946, 1733, 1847, 2026, 2212
Sfr274I	CTCGAG	1 cut(s)	2078
Sfr303I	CCGCGG	1 cut(s)	875
SgrBI	CCGCGG	1 cut(s)	875
SlaI	CTCGAG	1 cut(s)	2078
SmaI	CCCGGG	1 cut(s)	2048
SmiMI	CAYNNNNRTG	1 cut(s)	939
SmlI	CTYRAG	2 cut(s)	770, 2078
SmoI	CTYRAG	2 cut(s)	770, 2078
SspMI	CTAG	5 cut(s)	80, 1080, 1278, 1926, 1989
SstI	GAGCTC	3 cut(s)	1106, 1294, 1558
StyD4I	CCNGG	4 cut(s)	540, 1624, 2045, 2046
TaaI	ACNGT	7 cut(s)	224, 505, 1318, 1384, 1709, 1829, 2135
TaiI	ACGT	2 cut(s)	378, 1133
TaqI	TCGA	5 cut(s)	343, 500, 1430, 1994, 2079
TatI	WGTACW	1 cut(s)	684
TauI	GCSGC	6 cut(s)	76, 292, 424, 494, 877, 1977
Tru1I	TTAA	6 cut(s)	630, 771, 951, 1452, 1673, 2261
Tru9I	TTAA	6 cut(s)	630, 771, 951, 1452, 1673, 2261
TscAI	CASTG	3 cut(s)	134, 439, 1273
TseFI	GTSAC	1 cut(s)	191
TseI	GCWGC	6 cut(s)	34, 418, 449, 716, 978, 1808
Tsp45I	GTSAC	1 cut(s)	191
TspDTI	ATGAA	8 cut(s)	428, 838, 978, 1268, 1542, 1958, 2139, 2211
TspGWI	ACGGA	2 cut(s)	736, 1264
TspMI	CCCGGG	1 cut(s)	2046
TspRI	CASTG	3 cut(s)	134, 439, 1273
Tth111I	GACNNNGTC	1 cut(s)	2273
Van91I	CCANNNNNTGG	1 cut(s)	929
Vha464I	CTTAAG	1 cut(s)	770
XapI	RAATTY	1 cut(s)	2073
XhoI	CTCGAG	1 cut(s)	2078
XmaI	CCCGGG	1 cut(s)	2046
XmiI	GTMKAC	3 cut(s)	43, 343, 787
XmnI	GAANNNNTTC	3 cut(s)	330, 1488, 1870
XspI	CTAG	5 cut(s)	80, 1080, 1278, 1926, 1989
Zsp2I	ATGCAT	1 cut(s)	946

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.