Rorug05G0099500

Ubiquitin carboxyl-terminal hydrolase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000005
OG0005652
Physical Location & Seq
Forward (+)
8804432 .. 8805093
662 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug05G0099500.1

Sequence Viewer

Length: 369 bp
ATGGCTCAATCAAGGAGTGCTTCATCTCTTTCCATTCTGTTCCTTCTGCTCTTGTTCATCCACTTTCCTGGAGGGCCTATAAAGGTTGTTAGTGAGGATGAGAATAAGACCTGGTGTGTTGCTAAGCCTTCAGCAACTGATTCAGAGCTCTCTGCTAACATTCAGTTTGCCTGCAACCAATTGAAGGATTGCAAGGTGATACAAGAAGGAGGCTCATGCTATAACCCCAACTCACTCATTAACCATGCCTCAGTGGTGATGAATCTTTACTATAAAGCGGTAGGCCAAAACAGCTGGAATTGTGACTTCAGAGGATCTGCTCTCATCACTCAGACTGATCCAAGTTATGGTAAGTGCGAGTATGCATAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005829 GO:0005840 GO:0022626 GO:0032991 GO:0043226 GO:0043228 GO:0043229 GO:0043232 GO:0044424 GO:0044444 GO:0044445 GO:0044464 GO:1990904
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
UCH

Protein Analysis

122

Amino Acids

13.36

Weight (kDa)

6.04

Isoelectric Point (pI)

44.06

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
X8 PF07983 37 - 107 5.5e-22 X8 domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 1 cut(s) 347
AciI CCGC 1 cut(s) 278
AclWI GGATC 2 cut(s) 322, 332
AcuI CTGAAG 2 cut(s) 114, 292
AfiI CCNNNNNNNGG 2 cut(s) 184, 347
AgsI TTSAA 1 cut(s) 184
AjnI CCWGG 2 cut(s) 67, 110
AluBI AGCT 2 cut(s) 148, 294
AluI AGCT 2 cut(s) 148, 294
Alw21I GWGCWC 1 cut(s) 150
AlwI GGATC 2 cut(s) 322, 332
AlwNI CAGNNNCTG 1 cut(s) 137
AoxI GGCC 2 cut(s) 74, 283
AspS9I GGNCC 1 cut(s) 74
AsuHPI GGTGA 2 cut(s) 208, 268
BanII GRGCYC 1 cut(s) 150
Bbv12I GWGCWC 1 cut(s) 150
BciT130I CCWGG 2 cut(s) 69, 112
BlpI GCTNAGC 1 cut(s) 123
Bme1390I CCNGG 2 cut(s) 69, 112
BmgT120I GGNCC 1 cut(s) 74
BmrFI CCNGG 2 cut(s) 69, 112
BpmI CTGGAG 1 cut(s) 90
Bpu1102I GCTNAGC 1 cut(s) 123
Bsc4I CCNNNNNNNGG 2 cut(s) 184, 347
BseBI CCWGG 2 cut(s) 69, 112
BseGI GGATG 2 cut(s) 57, 103
BseLI CCNNNNNNNGG 2 cut(s) 184, 347
BseMII CTCAG 2 cut(s) 264, 344
BshFI GGCC 2 cut(s) 76, 285
BsiHKAI GWGCWC 1 cut(s) 150
BslI CCNNNNNNNGG 2 cut(s) 184, 347
BsnI GGCC 2 cut(s) 76, 285
Bsp1286I GDGCHC 1 cut(s) 150
Bsp143I GATC 2 cut(s) 314, 337
Bsp1720I GCTNAGC 1 cut(s) 123
BspACI CCGC 1 cut(s) 278
BspANI GGCC 2 cut(s) 76, 285
BspCNI CTCAG 2 cut(s) 263, 343
BspPI GGATC 2 cut(s) 322, 332
BssMI GATC 2 cut(s) 314, 337
Bst2UI CCWGG 2 cut(s) 69, 112
BstC8I GCNNGC 1 cut(s) 172
BstDEI CTNAG 3 cut(s) 123, 250, 330
BstF5I GGATG 2 cut(s) 57, 103
BstKTI GATC 2 cut(s) 317, 340
BstMBI GATC 2 cut(s) 314, 337
BstMWI GCNNNNNNNGC 1 cut(s) 291
BstNI CCWGG 2 cut(s) 69, 112
BstSCI CCNGG 2 cut(s) 67, 110
BstX2I RGATCY 1 cut(s) 314
BstXI CCANNNNNNTGG 1 cut(s) 68
BstYI RGATCY 1 cut(s) 314
BsuRI GGCC 2 cut(s) 76, 285
BtsCI GGATG 2 cut(s) 57, 103
BtsIMutI CAGTG 1 cut(s) 258
Cac8I GCNNGC 1 cut(s) 172
CaiI CAGNNNCTG 1 cut(s) 137
Cfr13I GGNCC 1 cut(s) 74
CsiI ACCWGGT 1 cut(s) 110
CviAII CATG 2 cut(s) 216, 245
CviJI RGCY 7 cut(s) 5, 76, 127, 148, 213, 285, 294
CviKI_1 RGCY 7 cut(s) 5, 76, 127, 148, 213, 285, 294
DdeI CTNAG 3 cut(s) 123, 250, 330
DpnI GATC 2 cut(s) 316, 339
DpnII GATC 2 cut(s) 314, 337
Ecl136II GAGCTC 1 cut(s) 148
Eco24I GRGCYC 1 cut(s) 150
Eco53kI GAGCTC 1 cut(s) 148
Eco57I CTGAAG 2 cut(s) 114, 292
EcoICRI GAGCTC 1 cut(s) 148
EcoO109I RGGNCCY 1 cut(s) 74
EcoRII CCWGG 2 cut(s) 67, 110
EcoT22I ATGCAT 1 cut(s) 367
EcoT38I GRGCYC 1 cut(s) 150
FaeI CATG 2 cut(s) 219, 248
FaiI YATR 8 cut(s) 80, 217, 222, 246, 273, 348, 363, 367
FalI AAGNNNNNCTT 1 cut(s) 36
FatI CATG 2 cut(s) 215, 244
FokI GGATG 2 cut(s) 44, 110
FriOI GRGCYC 1 cut(s) 150
GsuI CTGGAG 1 cut(s) 90
HaeIII GGCC 2 cut(s) 76, 285
Hin1II CATG 2 cut(s) 219, 248
HinfI GANTC 2 cut(s) 140, 262
HphI GGTGA 2 cut(s) 208, 268
Hpy188I TCNGA 3 cut(s) 145, 311, 333
HpyAV CCTTC 4 cut(s) 53, 138, 178, 200
HpyCH4V TGCA 3 cut(s) 174, 192, 365
HpyF10VI GCNNNNNNNGC 1 cut(s) 291
HpyF3I CTNAG 3 cut(s) 123, 250, 330
Hsp92II CATG 2 cut(s) 219, 248
Kzo9I GATC 2 cut(s) 314, 337
LpnPI CCDG 6 cut(s) 54, 81, 97, 124, 184, 280
MabI ACCWGGT 1 cut(s) 110
MaeIII GTNAC 1 cut(s) 302
MalI GATC 2 cut(s) 316, 339
MboI GATC 2 cut(s) 314, 337
MfeI CAATTG 1 cut(s) 179
MflI RGATCY 1 cut(s) 314
MhlI GDGCHC 1 cut(s) 150
MluCI AATT 2 cut(s) 179, 298
MnlI CCTC 5 cut(s) 65, 88, 203, 259, 305
Mph1103I ATGCAT 1 cut(s) 367
MseI TTAA 1 cut(s) 240
MspA1I CMGCKG 1 cut(s) 294
MspR9I CCNGG 2 cut(s) 69, 112
MunI CAATTG 1 cut(s) 179
MvaI CCWGG 2 cut(s) 69, 112
MwoI GCNNNNNNNGC 1 cut(s) 291
NdeII GATC 2 cut(s) 314, 337
NlaIII CATG 2 cut(s) 219, 248
NmuCI GTSAC 1 cut(s) 302
NsiI ATGCAT 1 cut(s) 367
PfeI GAWTC 2 cut(s) 140, 262
PflMI CCANNNNNTGG 1 cut(s) 347
PfoI TCCNGGA 1 cut(s) 67
Psp124BI GAGCTC 1 cut(s) 150
Psp6I CCWGG 2 cut(s) 67, 110
PspGI CCWGG 2 cut(s) 67, 110
PspPI GGNCC 1 cut(s) 74
PstNI CAGNNNCTG 1 cut(s) 137
PsuI RGATCY 1 cut(s) 314
PvuII CAGCTG 1 cut(s) 294
SacI GAGCTC 1 cut(s) 150
SaqAI TTAA 1 cut(s) 240
Sau3AI GATC 2 cut(s) 314, 337
Sau96I GGNCC 1 cut(s) 74
ScrFI CCNGG 2 cut(s) 69, 112
SduI GDGCHC 1 cut(s) 150
SetI ASST 5 cut(s) 87, 113, 150, 198, 296
SexAI ACCWGGT 1 cut(s) 110
Sse9I AATT 2 cut(s) 179, 298
SsiI CCGC 1 cut(s) 278
SstI GAGCTC 1 cut(s) 150
StyD4I CCNGG 2 cut(s) 67, 110
TasI AATT 2 cut(s) 179, 298
TfiI GAWTC 2 cut(s) 140, 262
Tru1I TTAA 1 cut(s) 240
Tru9I TTAA 1 cut(s) 240
TscAI CASTG 1 cut(s) 258
TseFI GTSAC 1 cut(s) 302
Tsp45I GTSAC 1 cut(s) 302
TspDTI ATGAA 3 cut(s) 12, 46, 275
TspRI CASTG 1 cut(s) 258
Van91I CCANNNNNTGG 1 cut(s) 347
Zsp2I ATGCAT 1 cut(s) 367
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.