MD11G1075100.v1.1
ERF Family

Belongs to the ABC transporter superfamily. ABCG family. PDR (TC 3.A.1.205) subfamily

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr11
OG0007618
Physical Location & Seq
Reverse (-)
6356201 .. 6365580
9380 bp
Loading structure...
UTR
Exon/CDS
Intron
MD11G1075100.v1.1.491

Sequence Viewer

Length: 4278 bp
ATGGCGGCGGCGTCGAACGGGAGCGAGTACTTCGAGATAGAGGCGGGGAACGAGTCGTTCGCTCGGCCTTCGAATGCCGAGTCCGTGGCGGAGGACGAGGAGGAGCTGATGTGGGCGGCGATCGGGAGGTTGCCGTCGCAGAGGCGGACGAACACGGCGCTGGTGAGGAAGAGGGAGTCCGATGGAAGAAGCGGTGGCGAAAAGGTGGAGACGGTCGACGTGAGGAAGCTGGATCGGTTCAATCGGGAGCTCGTCGTCAAAAAAGCGTTGGCCACTAACGATCAAGATAACTATCTCTTGCTCTCCGCTGTTAAAGAGCGTCTCGATAAAGTGGGATTGGAGGTCGCAAAGGTTGAAGTACGGTATGATAATCTGAAAGTGGTGGCAAACGTTCAGACTGGATCAAGAGCCTTGCCTACTCTAATCAACTATAGTCGTAATGCTTTAGAGGGTTTGTTAACTGGTTTGAGGATATTTCGACCTAAGAGACATTCTTTAACCATTTTGAACAACATTAGAGGCGTTGTTAAACCCGGAAGGATGACATTGCTTTTAGGTCCTCCAGGTTCTGGAAAATCCACTTTGCTTTTGGCTCTTGCAGGAAAACTTGACCCCAACTTAAAGAAAAGCGGTACCATTACCTACAATGGCCATAAGCTCAACGAGTTTTGCGTTCAAAGAACTGCAGCTTACATCAGCCAAACAGATAATCATATTGGAGAACTGACCGTAAGAGAAACGTTGGATTTTGCAGCTAGATGTCAAGGTGCAAGCGAAGGCTTTGCAGCATACATGAACGATTTGGTACGGTTAGAGAGGGAAAGGAACATAAGGCCGGATCCAGAAATTGATGCATATATGAAGGCATCATCTGTTGGCGGTAAAAAGCACAGTGTTTCAACAGATTATGTGTTGAAAGTGCTTGGTCTTGATGTATGTTCAGAGACAATTGTTGGAAACGAAATGGTAAGAGGCGTATCAGGTGGACAGAGGAAAAGGGTCACCACAGGAGAAATGGCTGTTGGCCCAAGAAAAGCCCTATTTATGGATGAAATTTCTACTGGACTAGATAGTTCTACTACATTCCAAATTGTAAAATGCACTCGGAACTTTGTTCATCTAATGGATGCGACAATACTAATGGCTCTTCTTCAGCCTGCGCCCGAGACATTTGATCTGTTTGATGACCTGGTGCTGTTATCAGAAGGGCATGTGGTTTATCATGGTCCTCGTGCCCAAGTATTGGAATTCTTCGAGTCATTAGGCTTTCGTCTACCCCCACGCAAGGGAGTTGCAGATTTTCTTCAAGAGGTGACTTCTAAAAAGGACCAATCTCAGTATTGGGCTGATAAATCGAAACCATATGTGTACCTTTCTGTTCCACAAATTGCCGAAGCCTTCAAAAATTCCAAATTTGGAAGGACTCTGGAGTCTGAGCTTTCGGATCCATATGATAAGTCTAATACTCATCCTGCAGCATTGGCAGAATCAAAATATGCAGTCTCAAGATGGGAACTTTGTAAAGCATGCTTTTCGCGAGAAATCCTGTTGATCAGCAGGCATAGGTTTCTTTATATATTTAGGACGTTCCAGGTTGCATTTGTTGGATTTGTTACATGCACAATGTTTCTGCGAACAAGACTACACCCGACAGATGAGGGACATGGAGAACTTTATCTTTCTTGCTTGTTTTTTGGGCTGGTGCACATGATGTTTAATGGATTTTCTGAGCTGCCTCTTATGATATCTCGGCTCCCAGTCTTTTACAAGCAAAGGGACAATTTCTTTCATCCTGCGTGGGCATGGTCTGTCGTGAGTTGGCTTCTGCGTATTCCTTACTCCGTTATTGAAGCTGTTGTATGGTCTTGTGTCGTATACTACACTGTTGGTTTTGCTCCTGCTGCTGGAAGGTTTTTCCGTTTCATGCTTTTACTGTTCTCAGTGCACCAAATGGCTTTGGGTCTCTTTCGGTTCATGGCTTCTATTACGCGAGATATGGTCATTGCCAATACATTTGGATCAGCTGCGCTACTAATTATATTCTTGCTGGGTGGATTCATCATTCCGAAAGCAAGCATTAAGTCATGGTGGGTTTGGGCCTTTTGGGTATCCCCACTATCTTATGGGCAACGAGCTATGTCCGTAAACGAATTTGCTGCTACAAGGTGGATGAAGAAATCTGCTGTTAATGATGACACAATCGGGCACAATATTCTTCACTCACACAGCTTACCAACTGGTGATCATTGGTATTGGATTGGAGTTGGTGCCTTATTGCTTTATGCCATACTTTTCAATGGCCTTGTGACTATGGCCTTGCTCTACCTAAATCCACTTAGGAAGAACCAGACAGTGGTTCCAGTTGACAACACAGAAGGGAGCCCTGCTGCAGATGTGGCTGAACCAAACCCAAAATCGACTGAAACTAGCAGCCCCAAAAAGGGAATGATCTTACCGTTTCAGCCATTGACAATGACTTTCCATAATGTTAATTACTTTGTTGACATGCCAAAGGAAATGAAGTCGCAAGGAATACCAGAAAATAAGTTGCAACTCTTGGCGAATGTGAGCGGAGTATTTTCACCAGGCGTTCTTACTGCTTTAGTTGGGTCTAGTGGAGCTGGTAAGACCACTTTGATGGATGTCCTTGCTGGTAGGAAAACTGGGGGATACATTGAAGGAGATATCAGAATATCTGGTTACCCAAAAGTGCAACGTACTTTTGCCAGAATATCGGGATATGTTGAGCAAAATGACATACATTCTCCTCAAGTTACAGTTGAGGAATCCCTGCTGTTTTCTTCTTCTCTTCGTCTGCCAAAGGAAGTCAGCAAAGAGAAAAGACTCGAATTTGTTGAAGAAGTAATGAGATTAGTAGAGCTTGATCCTCTAAGGCATGCTTTGGTCGGTTTGCCTGGCAGTTCAGGCTTATCAACAGAGCAAAGAAAACGCCTGACAATCGCTGTGGAGCTTGTTGCAAATCCTTCAATTATCTTTATGGATGAACCTACATCAGGACTCGATGCAAGGGCAGCAGCCATTGTGATGCGGACTGTTCGTAATACTGTTGATACAGGAAGAACGGTTGTCTGTACTATACATCAACCAAGTATTGATATATTTGAGGCATTTGATGAGCTTCTTCTTATGAAACGAGGGGGGCAAGTTATATATGGAGGAAAACTTGGTTTGCACTCGCAGACAATGATAAATTATTTTCAGGGAATCAACGGAATCAGTCCAATTCCCAGCGGGTACAATCCAGCAACCTGGATGCTTGAGGTTACTACACCTGCTTGTGAAGAGAGAATTGGTAAAGACTTCGCAAACGTTTACAGAAATTCAGACCAATACAGGGAGGTAGAAGAGTCCATCAAACAATTTAGTATTCCTCCATCTGGTTCAGAACCATTGAAGTTTGCATCGACATATTCTCAAACTACACTGTCGCAGTTTTTGATCTGCTTATGGAAACAAAATCTTGTATATTGGAGAAGTCCACATTATAATGCCATGAGGTTGATTTTTACAACGATCAGTGCACTGGTATTTGGTTCGGCATTTTGGAATGTTGGTACCAAAAGGGACTCACCTCAAGCACTAATGATGGTTATGGGAGCTCTTTATGCCGCTTGCTTGTTTCTTGGGGTCAATAACGCTTCTTCAGTGCAGCCAATTGTTTCAATTGAGAGGACAGTATTCTATCGAGAGAAAGCAGCCGGGATGTATTCTCCATTAGCTTATGCAGCAGCACAGGGCCTTGTCGAGATCCCATACATTGCTGTGCAGACAATAGTATACGGTGTAATCACATACTTCATGGTTCATTTTGAAAGGACGCTTAGAAAGTTTTTGCTCTATATTGTGTTCATGTTCCTCACATTCACCTACTTTACCTTCTATGGTATGGCGGCTGTTGGTCTCACATCTTCTCAACATCTTGCGGCTGTCGTCTCCTCTGCTTTCTACTCTTTGTGGAATCTCCTCTCGGGTTTTCTTGTCCCAAAGCCACATATCCCGGGATGGTGGATGTGGTTTTACTACATCTGTCCAGTGGCATGGACCCTTCGAGGTATTATCACCTCGCAACTTGGTGATGTGGAGACCACGATGAAAGGACCTACGTTTCAGGGCACCGTGAAAGAGTACTTGGAGGTCAATCTTGGTTATGGCCCTGGGATGATTGGAGTTTCAGTCGCCGTGCTTGTTTGCTTTTGCCTCCTTTTCTTCAGTGTCTTTGCGCTCTCAGTGAAGTTCCTCAACTTTCAGAAAAGATGA

Protein Analysis

1426

Amino Acids

159.24

Weight (kDa)

8.94

Isoelectric Point (pI)

38.69

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
ABC_tran PF00005 169 - 351 1.6e-18 ABC transporter
ABC2_membrane_7 PF19055 385 - 436 2.1e-06 ABC-2 type transporter
ABC2_membrane PF01061 506 - 718 3.6e-47 ABC-2 type transporter
PDR_assoc PF08370 723 - 786 4.8e-24 Plant PDR ABC transporter associated
ABC_tran PF00005 853 - 1005 2.1e-20 ABC transporter
ABC2_membrane PF01061 1151 - 1363 6.9e-56 ABC-2 type transporter
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 3507
AarI CACCTGC 1 cut(s) 3301
AasI GACNNNNNNGTC 1 cut(s) 1429
Acc36I ACCTGC 1 cut(s) 3301
Acc65I GGTACC 2 cut(s) 632, 3575
AccB1I GGYRCC 4 cut(s) 632, 2267, 3575, 4133
AccB7I CCANNNNNTGG 3 cut(s) 569, 1243, 2353
AccBSI CCGCTC 1 cut(s) 2571
AccI GTMKAC 4 cut(s) 216, 1273, 1875, 3798
AccII CGCG 2 cut(s) 1537, 1990
AclI AACGTT 3 cut(s) 390, 740, 3330
AclWI GGATC 9 cut(s) 240, 409, 833, 846, 1439, 1452, 2026, 2879, 3763
AcoI YGGCCR 2 cut(s) 270, 649
AcsI RAATTY 7 cut(s) 1053, 1247, 1405, 1412, 2150, 2849, 3340
AcuI CTGAAG 3 cut(s) 1136, 3648, 4213
AcyI GRCGYC 1 cut(s) 11
AjiI CACGTC 1 cut(s) 220
AjnI CCWGG 7 cut(s) 562, 1188, 1590, 2584, 2914, 3269, 4174
AjuI GAANNNNNNNTTGG 6 cut(s) 1005, 1037, 3294, 3326, 4133, 4165
Alw21I GWGCWC 5 cut(s) 252, 1707, 1947, 3544, 3622
Alw44I GTGCAC 3 cut(s) 1703, 1943, 3540
AlwI GGATC 9 cut(s) 240, 409, 833, 846, 1439, 1452, 2026, 2879, 3763
AlwNI CAGNNNCTG 1 cut(s) 569
Ama87I CYCGRG 3 cut(s) 1163, 3988, 4018
ApaLI GTGCAC 3 cut(s) 1703, 1943, 3540
ApoI RAATTY 7 cut(s) 1053, 1247, 1405, 1412, 2150, 2849, 3340
ArsI GACNNNNNNTTYG 2 cut(s) 65, 97
Asp718I GGTACC 2 cut(s) 632, 3575
AspLEI GCGC 4 cut(s) 160, 1162, 2029, 4243
AspS9I GGNCC 9 cut(s) 557, 1025, 1226, 1327, 2097, 3757, 4062, 4118, 4172
AsuC2I CCSGG 4 cut(s) 534, 3721, 4019, 4020
AsuHPI GGTGA 9 cut(s) 175, 994, 1324, 2252, 2574, 3582, 3877, 4072, 4106
AsuII TTCGAA 1 cut(s) 71
AvaI CYCGRG 3 cut(s) 1163, 3988, 4018
AvaII GGWCC 5 cut(s) 557, 1226, 1327, 4062, 4118
BaeGI GKGCMC 6 cut(s) 1237, 1707, 1947, 2208, 3544, 4136
BalI TGGCCA 2 cut(s) 272, 651
BamHI GGATCC 2 cut(s) 838, 1444
BanI GGYRCC 4 cut(s) 632, 2267, 3575, 4133
BanII GRGCYC 3 cut(s) 252, 2384, 3622
BauI CACGAG 1 cut(s) 1230
Bbv12I GWGCWC 5 cut(s) 252, 1707, 1947, 3544, 3622
BccI CCATC 7 cut(s) 176, 1503, 2632, 3380, 3403, 3601, 4017
BceAI ACGGC 3 cut(s) 118, 171, 4184
BcgI CGANNNNNNTGC 6 cut(s) 764, 798, 1515, 1549, 2138, 2172
BciT130I CCWGG 7 cut(s) 564, 1190, 1592, 2586, 2916, 3271, 4176
BciVI GTATCC 2 cut(s) 2119, 2663
BclI TGATCA 2 cut(s) 1551, 2242
BcnI CCSGG 4 cut(s) 534, 3721, 4019, 4020
BfaI CTAG 4 cut(s) 756, 1067, 2427, 2613
BfmI CTRYAG 4 cut(s) 430, 684, 1473, 2388
BfoI RGCGCY 1 cut(s) 161
BfuAI ACCTGC 1 cut(s) 3301
BfuI GTATCC 2 cut(s) 2119, 2663
BmcAI AGTACT 2 cut(s) 29, 4148
Bme18I GGWCC 5 cut(s) 557, 1226, 1327, 4062, 4118
BmeT110I CYCGRG 3 cut(s) 1163, 3988, 4018
BmgBI CACGTC 1 cut(s) 220
BmgT120I GGNCC 9 cut(s) 557, 1025, 1226, 1327, 2097, 3757, 4062, 4118, 4172
BmrI ACTGGG 2 cut(s) 1751, 2673
BmsI GCATC 7 cut(s) 841, 875, 1117, 3013, 3036, 3264, 3431
BmuI ACTGGG 2 cut(s) 1751, 2673
BpmI CTGGAG 2 cut(s) 546, 1448
Bpu14I TTCGAA 1 cut(s) 71
BpuEI CTTGAG 4 cut(s) 1489, 2754, 3299, 3579
BpuMI CCSGG 4 cut(s) 534, 3721, 4019, 4020
BsaBI GATNNNNATC 1 cut(s) 291
BsaHI GRCGYC 1 cut(s) 11
BsaI GGTCTC 3 cut(s) 1967, 3926, 4097
BsaJI CCNNGG 4 cut(s) 84, 4018, 4174, 4175
BsaXI ACNNNNNCTCC 4 cut(s) 95, 125, 4179, 4209
Bse1I ACTGG 9 cut(s) 403, 466, 1066, 1757, 2242, 2360, 2668, 3549, 4052
Bse3DI GCAATG 3 cut(s) 545, 2001, 3777
Bse8I GATNNNNATC 1 cut(s) 291
BseBI CCWGG 7 cut(s) 564, 1190, 1592, 2586, 2916, 3271, 4176
BseDI CCNNGG 4 cut(s) 84, 4018, 4174, 4175
BseJI GATNNNNATC 1 cut(s) 291
BseMI GCAATG 3 cut(s) 545, 2001, 3777
BseMII CTCAG 5 cut(s) 1349, 1425, 1719, 1953, 4260
BseNI ACTGG 9 cut(s) 403, 466, 1066, 1757, 2242, 2360, 2668, 3549, 4052
BseRI GAGGAG 5 cut(s) 113, 116, 2757, 3946, 3974
BseSI GKGCMC 6 cut(s) 1237, 1707, 1947, 2208, 3544, 4136
BseYI CCCAGC 2 cut(s) 2047, 3248
BsgI GTGCAG 2 cut(s) 3689, 3806
Bsh1236I CGCG 2 cut(s) 1537, 1990
Bsh1285I CGRYCG 2 cut(s) 123, 216
BshNI GGYRCC 4 cut(s) 632, 2267, 3575, 4133
BsiEI CGRYCG 2 cut(s) 123, 216
BsiHKAI GWGCWC 5 cut(s) 252, 1707, 1947, 3544, 3622
BsiHKCI CYCGRG 3 cut(s) 1163, 3988, 4018
BsiSI CCGG 4 cut(s) 534, 836, 3720, 4019
BslFI GGGAC 4 cut(s) 1674, 1790, 3599, 3986
BsmBI CGTCTC 3 cut(s) 203, 326, 3958
BsmFI GGGAC 4 cut(s) 1674, 1790, 3599, 3986
BsmI GAATGC 1 cut(s) 79
Bso31I GGTCTC 3 cut(s) 1967, 3926, 4097
BsoBI CYCGRG 3 cut(s) 1163, 3988, 4018
Bsp119I TTCGAA 1 cut(s) 71
Bsp1286I GDGCHC 9 cut(s) 252, 1237, 1707, 1947, 2208, 2384, 3544, 3622, 4136
Bsp68I TCGCGA 1 cut(s) 1537
BspCNI CTCAG 5 cut(s) 1348, 1426, 1720, 1952, 4259
BspFNI CGCG 2 cut(s) 1537, 1990
BspMAI CTGCAG 3 cut(s) 688, 1477, 2392
BspMI ACCTGC 1 cut(s) 3301
BspPI GGATC 9 cut(s) 240, 409, 833, 846, 1439, 1452, 2026, 2879, 3763
BspQI GCTCTTC 1 cut(s) 1152
BspT104I TTCGAA 1 cut(s) 71
BspT107I GGYRCC 4 cut(s) 632, 2267, 3575, 4133
BspTNI GGTCTC 3 cut(s) 1967, 3926, 4097
BsrBI CCGCTC 1 cut(s) 2571
BsrDI GCAATG 3 cut(s) 545, 2001, 3777
BsrI ACTGG 9 cut(s) 403, 466, 1066, 1757, 2242, 2360, 2668, 3549, 4052
BssECI CCNNGG 4 cut(s) 84, 4018, 4174, 4175
BssNAI GTATAC 2 cut(s) 1876, 3799
BssNI GRCGYC 1 cut(s) 11
BssSI CACGAG 1 cut(s) 1230
Bst1107I GTATAC 2 cut(s) 1876, 3799
Bst2BI CACGAG 1 cut(s) 1230
Bst2UI CCWGG 7 cut(s) 564, 1190, 1592, 2586, 2916, 3271, 4176
Bst6I CTCTTC 5 cut(s) 164, 1152, 2814, 3297, 3360
BstACI GRCGYC 1 cut(s) 11
BstBI TTCGAA 1 cut(s) 71
BstC8I GCNNGC 7 cut(s) 772, 1158, 1528, 1559, 2074, 2898, 3634
BstDEI CTNAG 9 cut(s) 483, 1335, 1434, 1728, 1939, 2336, 2891, 3842, 4246
BstDSI CCRYGG 1 cut(s) 84
BstEII GGTNACC 2 cut(s) 1000, 2699
BstENI CCTNNNNNAGG 1 cut(s) 3012
BstFNI CGCG 2 cut(s) 1537, 1990
BstH2I RGCGCY 1 cut(s) 161
BstHHI GCGC 4 cut(s) 160, 1162, 2029, 4243
BstMCI CGRYCG 2 cut(s) 123, 216
BstMWI GCNNNNNNNGC 7 cut(s) 1481, 1901, 2396, 2925, 3032, 3626, 3746
BstNI CCWGG 7 cut(s) 564, 1190, 1592, 2586, 2916, 3271, 4176
BstNSI RCATGY 5 cut(s) 1214, 1530, 1620, 2509, 2900
BstPI GGTNACC 2 cut(s) 1000, 2699
BstSFI CTRYAG 4 cut(s) 430, 684, 1473, 2388
BstSLI GKGCMC 6 cut(s) 1237, 1707, 1947, 2208, 3544, 4136
BstUI CGCG 2 cut(s) 1537, 1990
BstX2I RGATCY 3 cut(s) 838, 1444, 3768
BstXI CCANNNNNNTGG 3 cut(s) 2638, 3270, 4059
BstYI RGATCY 3 cut(s) 838, 1444, 3768
BstZ17I GTATAC 2 cut(s) 1876, 3799
BsuI GTATCC 2 cut(s) 2119, 2663
BtgI CCRYGG 1 cut(s) 84
BtrI CACGTC 1 cut(s) 220
BtuMI TCGCGA 1 cut(s) 1537
BveI ACCTGC 1 cut(s) 3301
Cac8I GCNNGC 7 cut(s) 772, 1158, 1528, 1559, 2074, 2898, 3634
CaiI CAGNNNCTG 1 cut(s) 569
CfoI GCGC 4 cut(s) 160, 1162, 2029, 4243
Cfr13I GGNCC 9 cut(s) 557, 1025, 1226, 1327, 2097, 3757, 4062, 4118, 4172
Cfr9I CCCGGG 1 cut(s) 4018
CseI GACGC 2 cut(s) 308, 3847
CsiI ACCWGGT 1 cut(s) 1188
CspCI CAANNNNNGTGG 2 cut(s) 2946, 2981
DdeI CTNAG 9 cut(s) 483, 1335, 1434, 1728, 1939, 2336, 2891, 3842, 4246
DrdI GACNNNNNNGTC 1 cut(s) 1429
DseDI GACNNNNNNGTC 1 cut(s) 1429
EaeI YGGCCR 2 cut(s) 270, 649
Eam1104I CTCTTC 5 cut(s) 164, 1152, 2814, 3297, 3360
EarI CTCTTC 5 cut(s) 164, 1152, 2814, 3297, 3360
EciI GGCGGA 2 cut(s) 104, 160
Ecl136II GAGCTC 2 cut(s) 250, 3620
Eco24I GRGCYC 3 cut(s) 252, 2384, 3622
Eco31I GGTCTC 3 cut(s) 1967, 3926, 4097
Eco32I GATATC 2 cut(s) 1746, 2686
Eco47I GGWCC 5 cut(s) 557, 1226, 1327, 4062, 4118
Eco53kI GAGCTC 2 cut(s) 250, 3620
Eco57I CTGAAG 3 cut(s) 1136, 3648, 4213
Eco88I CYCGRG 3 cut(s) 1163, 3988, 4018
Eco91I GGTNACC 2 cut(s) 1000, 2699
EcoICRI GAGCTC 2 cut(s) 250, 3620
EcoNI CCTNNNNNAGG 1 cut(s) 3012
EcoO109I RGGNCCY 3 cut(s) 557, 3757, 4118
EcoO65I GGTNACC 2 cut(s) 1000, 2699
EcoRI GAATTC 1 cut(s) 1247
EcoRII CCWGG 7 cut(s) 562, 1188, 1590, 2584, 2914, 3269, 4174
EcoRV GATATC 2 cut(s) 1746, 2686
EcoT22I ATGCAT 1 cut(s) 856
EcoT38I GRGCYC 3 cut(s) 252, 2384, 3622
Esp3I CGTCTC 3 cut(s) 203, 326, 3958
FalI AAGNNNNNCTT 2 cut(s) 2884, 2916
FaqI GGGAC 4 cut(s) 1674, 1790, 3599, 3986
FauI CCCGC 2 cut(s) 37, 3245
FauNDI CATATG 2 cut(s) 1363, 1450
FbaI TGATCA 2 cut(s) 1551, 2242
FblI GTMKAC 4 cut(s) 216, 1273, 1875, 3798
FriOI GRGCYC 3 cut(s) 252, 2384, 3622
FspBI CTAG 4 cut(s) 756, 1067, 2427, 2613
GlaI GCGC 4 cut(s) 159, 1161, 2028, 4242
GsaI CCCAGC 2 cut(s) 2051, 3252
GsuI CTGGAG 2 cut(s) 546, 1448
HaeII RGCGCY 1 cut(s) 161
HapII CCGG 4 cut(s) 534, 836, 3720, 4019
HgaI GACGC 2 cut(s) 308, 3847
HhaI GCGC 4 cut(s) 160, 1162, 2029, 4243
Hin1I GRCGYC 1 cut(s) 11
Hin6I GCGC 4 cut(s) 158, 1160, 2027, 4241
HinP1I GCGC 4 cut(s) 158, 1160, 2027, 4241
HincII GTYRAC 4 cut(s) 217, 459, 2365, 2503
HindII GTYRAC 4 cut(s) 217, 459, 2365, 2503
HpaI GTTAAC 1 cut(s) 459
HpaII CCGG 4 cut(s) 534, 836, 3720, 4019
HphI GGTGA 9 cut(s) 175, 994, 1324, 2252, 2574, 3582, 3877, 4072, 4106
Hpy99I CGWCG 4 cut(s) 16, 139, 221, 257
HpyCH4IV ACGT 7 cut(s) 219, 390, 740, 1586, 2716, 3330, 4124
HpyF10VI GCNNNNNNNGC 7 cut(s) 1481, 1901, 2396, 2925, 3032, 3626, 3746
HpyF3I CTNAG 9 cut(s) 483, 1335, 1434, 1728, 1939, 2336, 2891, 3842, 4246
HpySE526I ACGT 7 cut(s) 219, 390, 740, 1586, 2716, 3330, 4124
Hsp92I GRCGYC 1 cut(s) 11
HspAI GCGC 4 cut(s) 158, 1160, 2027, 4241
KpnI GGTACC 2 cut(s) 636, 3579
Ksp22I TGATCA 2 cut(s) 1551, 2242
KspAI GTTAAC 1 cut(s) 459
LguI GCTCTTC 1 cut(s) 1152
LmnI GCTCC 9 cut(s) 21, 103, 247, 1758, 1900, 2379, 2618, 2968, 3617
LweI GCATC 7 cut(s) 841, 875, 1117, 3013, 3036, 3264, 3431
MabI ACCWGGT 1 cut(s) 1188
MaeI CTAG 4 cut(s) 756, 1067, 2427, 2613
MaeII ACGT 7 cut(s) 219, 390, 740, 1586, 2716, 3330, 4124
MaeIII GTNAC 7 cut(s) 1000, 1312, 1612, 2305, 2699, 2773, 3283
MbiI CCGCTC 1 cut(s) 2571
MfeI CAATTG 3 cut(s) 948, 3675, 3684
MflI RGATCY 3 cut(s) 838, 1444, 3768
MhlI GDGCHC 9 cut(s) 252, 1237, 1707, 1947, 2208, 2384, 3544, 3622, 4136
MlsI TGGCCA 2 cut(s) 272, 651
MluNI TGGCCA 2 cut(s) 272, 651
MmeI TCCRAC 3 cut(s) 723, 934, 1585
Mox20I TGGCCA 2 cut(s) 272, 651
Mph1103I ATGCAT 1 cut(s) 856
MscI TGGCCA 2 cut(s) 272, 651
MseI TTAA 9 cut(s) 312, 458, 497, 528, 620, 1716, 2079, 2187, 2490
MslI CAYNNNNRTG 4 cut(s) 2636, 3044, 3507, 3782
Msp20I TGGCCA 2 cut(s) 272, 651
MspA1I CMGCKG 3 cut(s) 308, 2024, 3252
MspI CCGG 4 cut(s) 534, 836, 3720, 4019
MunI CAATTG 3 cut(s) 948, 3675, 3684
Mva1269I GAATGC 1 cut(s) 79
MvaI CCWGG 7 cut(s) 564, 1190, 1592, 2586, 2916, 3271, 4176
MvnI CGCG 2 cut(s) 1537, 1990
MwoI GCNNNNNNNGC 7 cut(s) 1481, 1901, 2396, 2925, 3032, 3626, 3746
NciI CCSGG 4 cut(s) 534, 3721, 4019, 4020
NdeI CATATG 2 cut(s) 1363, 1450
NmeAIII GCCGAG 3 cut(s) 43, 103, 1729
NmuCI GTSAC 3 cut(s) 1000, 1312, 2305
NruI TCGCGA 1 cut(s) 1537
NsiI ATGCAT 1 cut(s) 856
NspI RCATGY 5 cut(s) 1214, 1530, 1620, 2509, 2900
NspV TTCGAA 1 cut(s) 71
PaeI GCATGC 2 cut(s) 1530, 2900
PaqCI CACCTGC 1 cut(s) 3301
PasI CCCWGGG 1 cut(s) 4175
PciSI GCTCTTC 1 cut(s) 1152
PcsI WCGNNNNNNNCGW 1 cut(s) 669
PctI GAATGC 1 cut(s) 79
PfeI GAWTC 6 cut(s) 1487, 2055, 2786, 3225, 3234, 3979
PflMI CCANNNNNTGG 3 cut(s) 569, 1243, 2353
Ple19I CGATCG 1 cut(s) 123
PpuMI RGGWCCY 2 cut(s) 557, 4118
PsiI TTATAA 1 cut(s) 3507
Psp124BI GAGCTC 2 cut(s) 252, 3622
Psp1406I AACGTT 3 cut(s) 390, 740, 3330
Psp5II RGGWCCY 2 cut(s) 557, 4118
Psp6I CCWGG 7 cut(s) 562, 1188, 1590, 2584, 2914, 3269, 4174
PspEI GGTNACC 2 cut(s) 1000, 2699
PspFI CCCAGC 2 cut(s) 2047, 3248
PspGI CCWGG 7 cut(s) 562, 1188, 1590, 2584, 2914, 3269, 4174
PspPI GGNCC 9 cut(s) 557, 1025, 1226, 1327, 2097, 3757, 4062, 4118, 4172
PspPPI RGGWCCY 2 cut(s) 557, 4118
PstI CTGCAG 3 cut(s) 688, 1477, 2392
PstNI CAGNNNCTG 1 cut(s) 569
PsuI RGATCY 3 cut(s) 838, 1444, 3768
PvuI CGATCG 1 cut(s) 123
PvuII CAGCTG 1 cut(s) 2024
RruI TCGCGA 1 cut(s) 1537
RseI CAYNNNNRTG 4 cut(s) 2636, 3044, 3507, 3782
SacI GAGCTC 2 cut(s) 252, 3622
SalI GTCGAC 1 cut(s) 215
SapI GCTCTTC 1 cut(s) 1152
SaqAI TTAA 9 cut(s) 312, 458, 497, 528, 620, 1716, 2079, 2187, 2490
Sau96I GGNCC 9 cut(s) 557, 1025, 1226, 1327, 2097, 3757, 4062, 4118, 4172
ScaI AGTACT 2 cut(s) 29, 4148
SduI GDGCHC 9 cut(s) 252, 1237, 1707, 1947, 2208, 2384, 3544, 3622, 4136
SexAI ACCWGGT 1 cut(s) 1188
SfaNI GCATC 7 cut(s) 841, 875, 1117, 3013, 3036, 3264, 3431
SfcI CTRYAG 4 cut(s) 430, 684, 1473, 2388
SfuI TTCGAA 1 cut(s) 71
SinI GGWCC 5 cut(s) 557, 1226, 1327, 4062, 4118
SmaI CCCGGG 1 cut(s) 4020
SmiMI CAYNNNNRTG 4 cut(s) 2636, 3044, 3507, 3782
SmlI CTYRAG 4 cut(s) 1504, 2769, 3278, 3594
SmoI CTYRAG 4 cut(s) 1504, 2769, 3278, 3594
SphI GCATGC 2 cut(s) 1530, 2900
SspI AATATT 1 cut(s) 2212
SspMI CTAG 4 cut(s) 756, 1067, 2427, 2613
SstI GAGCTC 2 cut(s) 252, 3622
TaiI ACGT 7 cut(s) 222, 393, 743, 1589, 2719, 3333, 4127
TatI WGTACW 3 cut(s) 27, 3092, 4146
TauI GCSGC 6 cut(s) 8, 11, 119, 3632, 3914, 3947
TfiI GAWTC 6 cut(s) 1487, 2055, 2786, 3225, 3234, 3979
Tru1I TTAA 9 cut(s) 312, 458, 497, 528, 620, 1716, 2079, 2187, 2490
Tru9I TTAA 9 cut(s) 312, 458, 497, 528, 620, 1716, 2079, 2187, 2490
TseFI GTSAC 3 cut(s) 1000, 1312, 2305
Tsp45I GTSAC 3 cut(s) 1000, 1312, 2305
TspGWI ACGGA 5 cut(s) 73, 1831, 1907, 2131, 3246
TspMI CCCGGG 1 cut(s) 4018
Van91I CCANNNNNTGG 3 cut(s) 569, 1243, 2353
VneI GTGCAC 3 cut(s) 1703, 1943, 3540
VpaK11BI GGWCC 5 cut(s) 557, 1226, 1327, 4062, 4118
XagI CCTNNNNNAGG 1 cut(s) 3012
XapI RAATTY 7 cut(s) 1053, 1247, 1405, 1412, 2150, 2849, 3340
XceI RCATGY 5 cut(s) 1214, 1530, 1620, 2509, 2900
XcmI CCANNNNNNNNNTGG 2 cut(s) 586, 1012
XmaI CCCGGG 1 cut(s) 4018
XmiI GTMKAC 4 cut(s) 216, 1273, 1875, 3798
XspI CTAG 4 cut(s) 756, 1067, 2427, 2613
ZrmI AGTACT 2 cut(s) 29, 4148
Zsp2I ATGCAT 1 cut(s) 856
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.