Rw4G032920
ERF Family

Belongs to the ABC transporter superfamily. ABCG family. PDR (TC 3.A.1.205) subfamily

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr4
OG0007618
Physical Location & Seq
Reverse (-)
59977408 .. 59984892
7485 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw4G032920.1

Sequence Viewer

Length: 3894 bp
ATGGCGGCGTCGCAGACCATCGACGTCCGGAATCTCAATCGCCATAACCGCGAGCTCGTCCTCAAGAAAGCCCTCGCCACTGACGATCAAGACAACCAGCACCTCCTCGCCGGAATAAAAGAACGCCTCGACAGAGTGGGATTGGAGGTTCCGACAGTAGAAGTACGATTTGAGAACCTGAATATCGTGGCAGACGTTCAAACAGGATCAAGAGCTTTGCCTACTCTCATCAACTATATTCGTGATGGTGTAGAGGGTATTTTAACTAGTTTCAGGATTGTTAAACCCAAGAGACGGTCTTTAACGATTTTGAACAACATCAGCGGCCTTATCAAACCCGGAAGAATGACTTTGCTTTTAGGACCCCCAAGTTCTGGCAAATCCACTTTGCTCATGAATCTTGCAGGAAAACTAGACCGCAACTTAAAGAATAGTGGCACCATCACATATAATGGCCATAAGCTTGATGAGTTCTGCGTTCAAAGAACGTCTGCTTACATAAGCCAAACAGATAATCACATTGCAGAACTCACCCAGGAAAGACAAATAAGGCCGGATCCAAATATTGCTGCCTTTATGAAGGCATCATCTGTTGGTAAAATTTCAACAGATTATGTTCTTAAAGTGCTCGGTCTTGATGTCTGCTCAGATACAATTGTCGGAAACAACATGATAAGAGGGGTTTCTGGGGGACAGAGGAAAAGGGTTACTACAGGTGAAATGACAGTTGGACCAAGAAAAGCCCTGTTTATGGATGAAATATCTACTGGACTGGATAGTTCTACTACATACCAAATTGTAAAATGTATTGGGAATTTTGTTCATCAGATGGAAGCTACAGTACTAATGGCTCTGCTTCAACCTGCACCTGAGACATTTGAATTGTTTGATGATCTAGTGCTCCTATCCGAAGGTCACGTGGTTTATCAAGGCCCTCGAGCAGAAGTGTTGGAGTTCTTCGAGTCATTAGGATTCAAACTACCCCCACGAAAGGGTATTGCGGATTTTCTTCAAGAGGTAACTTCTAAAAAGGATCAAGCTCAGTACTGGGCTGATTGTTCGAAACCATACACATACCTTTCTGTTTCAGAAATTGCAGAAGCCTTTAAGGAGTCCAGGTTTGGAAGGTCTCTGGATGACACTCTCTCTGCTCCATATCATAAATCTAGCTGTCTTCCTTCGGCTTTGCCAAAAACTAAATATGCAACCTCAAAATGGGAGCTTTGTAAAGCATGCTTTTCGCGAGAACTACTGTTGATCAGCAGGCATAGGTTTCTTTACACATTTAGGACCTGCCAGGTTGCATTTGTTGGATTTGTGACATGCACAACATTTCCTAGATGGCAATTACACCCCACAGATGAAATAAATGGCAACCTTTATCTTTCTTGCTTGTTCTTTGGGTTAGTGCACATGATGTTCAATGGGCATTCCGAGCTACCTCTTATGATATCTCAGCTCCCAGTCTTTTACAAGCAAAGGGATAATTACTTTCATCCTGCGTGGGCATGGTCTGGTGTTAATTTGCTTCTGCTTGGTTTTGCCCCTGGTGCTGGGAAATTTTTCCGTTTCATGTTTTTGCTATTTTCTGTGCACCAAATGGCTTTGGGTCTCTTCCGGACATTGGCATCTCTTGCAAGAGATATGGTCATTGCCAATACATTTGGATCAGCTGCATTACTTGTTATATTTTTGTTGGGTGGATTCGTTATTCCCAAAGATAGCATTAAGCCATGGTGGCAATGGGCCTTTTGGGTATCACCACTATCATATGGACAACGCGCTCTTTCTGTCAATGAATTTACTGCTGTAAGGTGGATCAAGATAGCTCCTTTAACAAACAGAACAATTGGGCAAAATGTCCTCCAAGAACACAACTTACCAAATGGTGATTATTGGTATTGGATTGGAGTTGGTGGTCTTTTAGCTTATGCATTGCTTTTCAATTGCACAGTGACTCTGGCCTTGACGTACCTAAATCCACTAAGGAAGTACCAGACAGTGCTTCCACTTGATGACACAGAAGGGACTCCTGCTGCAGATGGGGGTAAAAATAAGAGTGTGTCTAGCCCAAAATCAGCTGGAGGTAACAGCCCAGTAAAGGGAATGATCTTACCATTTCAGCCATTAACAATGACTTTCCACAATGTTACCTACTCTGTTCACAAGCCAAAGGAGATGAAGTCACAAGGTATAGCAGAATCGAAGTTGCCACTCTTGTCGAAGGTGAGCGGAGTATTCTCACCAGGTATTCTTACAGCTTTAGTTGGGTCTAGTGGAGCTGGAAAGACCACTTTGATGGACGTCCTTGCTGGTAGGAAAACTGGTGGATATATAGAAGGAGATATTAAGATATCTGGTTACCCGAGAGAGCAACGTACTTTTGCTAGAGTAGCAGGATATGTTGAGCAAAATGACATACATTCTCCTCAAGTTACAGTTGAGGAGAGTCTATGGTTTTCTTCTGCTCTTCGCCTTCCAAAGGAAGTCAGCACAGAGACAAGACTCGTGTTTGTCGAAGAAGTAATGGAACTAGTAGAGCTTGATACTCTAAGGCATGCTTTGGTTGGTGTGCCAGGCAGTTCAGGCTTATCAACAGAGCAAAGAAAACGCTTAACAATCGCTGTTGAGCTTGTTGCGAACCCTTCAATTATCTTTATGGATGAACCCACATCTGGACTGGACGCAAGGGCAGCAGCCATTGTGATGAGGACTGTTCGTAATACTGTTGATACAGGAAGAACGGTGGTCTGCACTATACACCAACCAAGTATCGACATTTTTGAGGCATTTGACGAGCTTCTTCTTATGAAACGAGGGGGGCAAGTTATATATGGAGGAAAGCTAGGCCAGCACTCCCAGACAATGATAAACTACTTTCAGGAGATTAATGGAATCACTCCGATTCCAAGCGGGTACAATCCAGCAACCTGGATGCTGGAGGTAACCACACCGTCTTTTGAACAGAGAATTGGAGAAGACTTCGCTACAATATACAGGAATTCAGAGCAGTACAGGGAGGTGGAAGAGTCCATCAAACAGTTGAGTACTCCTCCAACGGACTCAAAACCATTAAAGTTTGCTTCCACATATTCACAGAACACATTGGCTCAATTTAGGAACTGCTTATGGAAACAGAATCTCGTGTATTGGAGAAGTCCACAATATAATGCGATGAGGTTAATCTTTACTATCCTTGCTGCATTGATACTGGGCTCGGCATTTTGGGATGTCGCTCTTTATTCTGCTTGTTTGTTTCTTGGGGTCAATAACGCTTCTTCAGTTCAGCCGATTGTTTCGATTGAGAGGACGGTATTTTATCGAGAGAAAGCAGCTGGAATGTATTCTCCTTTGGCTTATGCAGCGGCCCAGGGCCTTATAGAGATCCCATACATTGCTGTGCAGACAATAGTATACGGGGTGATCACATACTTCATGATCAATTTTGAAAGGACAATCACAAAATTCTTGCTCTATATTCTGATCATGTTCCTCACTTTCGTATATTTCACTCTTTTCGGCATGGCGGCTGTTGCTGTCACAGCTTCTCAACCACTGGCAGCTGCCATCTCTGCTGCATTTTACTCATTGTGGAATCTATTCTCTGGTTTCCTCGTCCCCAAACCAAACATCCCGGTATGGTGGCTTTGGTTTTACTACATCTCCCCAGTTGCATGGACACTCAGAGGTATTATCACTTCCCAACTTGGTGATGTGGAGACCATTCTTGTCGGACCTGCGTTTAAGGGCACCTTGAAAGAATACTTGGAAGTCAGCCTTGGCTATGGCCCTGGGATGATTGGAGTTTCAGTAGCTGTTCTTCTGGGCTTCAACATCCTTTTGTTCAGTATCTTTGCCTTCTCCATCAAATTTCTCAACTTCCAGAGAAGATAG

Protein Analysis

1297

Amino Acids

144.57

Weight (kDa)

8.94

Isoelectric Point (pI)

36.12

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
ABC_trans_N PF14510 28 - 78 4.2e-06 ABC-transporter N-terminal
ABC_tran PF00005 104 - 253 6.1e-16 ABC transporter
ABC2_membrane_7 PF19055 287 - 337 5.2e-06 ABC-2 type transporter
ABC2_membrane PF01061 408 - 504 2e-11 ABC-2 type transporter
ABC2_membrane PF01061 507 - 601 5e-24 ABC-2 type transporter
PDR_assoc PF08370 606 - 668 2.4e-22 Plant PDR ABC transporter associated
ABC_tran PF00005 741 - 892 1.3e-20 ABC transporter
ABC2_membrane PF01061 1038 - 1235 5.1e-52 ABC-2 type transporter
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 2 cut(s) 27, 2307
Acc36I ACCTGC 3 cut(s) 871, 1301, 3745
AccB1I GGYRCC 2 cut(s) 437, 3749
AccB7I CCANNNNNTGG 1 cut(s) 374
AccBSI CCGCTC 1 cut(s) 2232
AccI GTMKAC 1 cut(s) 3414
AccII CGCG 3 cut(s) 51, 1243, 1782
AccIII TCCGGA 2 cut(s) 27, 1617
AciI CCGC 9 cut(s) 5, 49, 324, 418, 1001, 2232, 2913, 3365, 3527
AclWI GGATC 7 cut(s) 214, 551, 564, 1041, 1675, 1826, 3379
AcoI YGGCCR 1 cut(s) 454
AcsI RAATTY 7 cut(s) 600, 814, 1559, 1799, 3001, 3464, 3869
AcuI CTGAAG 1 cut(s) 3264
AcvI CACGTG 1 cut(s) 919
AcyI GRCGYC 3 cut(s) 8, 24, 2304
AfaI GTAC 9 cut(s) 165, 843, 1046, 1973, 1994, 2380, 2918, 3014, 3049
AhlI ACTAGT 2 cut(s) 266, 2533
AjnI CCWGG 9 cut(s) 534, 1115, 1296, 1546, 2245, 2575, 2930, 3369, 3790
AjuI GAANNNNNNNTTGG 8 cut(s) 711, 743, 2955, 2987, 3749, 3762, 3781, 3794
AloI GAACNNNNNNTCC 2 cut(s) 132, 164
Alw21I GWGCWC 5 cut(s) 57, 630, 903, 1413, 1596
Alw26I GTCTC 6 cut(s) 286, 866, 1134, 1616, 2492, 3713
Alw44I GTGCAC 2 cut(s) 1409, 1592
AlwI GGATC 7 cut(s) 214, 551, 564, 1041, 1675, 1826, 3379
AlwNI CAGNNNCTG 1 cut(s) 3815
Ama87I CYCGRG 2 cut(s) 936, 2365
Aor13HI TCCGGA 2 cut(s) 27, 1617
ApaLI GTGCAC 2 cut(s) 1409, 1592
ApoI RAATTY 7 cut(s) 600, 814, 1559, 1799, 3001, 3464, 3869
AseI ATTAAT 1 cut(s) 2889
Asp700I GAANNNNTTC 3 cut(s) 1562, 3343, 3599
AspLEI GCGC 1 cut(s) 1784
AspS9I GGNCC 9 cut(s) 362, 731, 932, 1290, 1746, 3367, 3373, 3734, 3788
AsuC2I CCSGG 2 cut(s) 339, 3635
AsuHPI GGTGA 8 cut(s) 523, 728, 1752, 1901, 2235, 2239, 3433, 3722
AsuII TTCGAA 1 cut(s) 1061
AvaI CYCGRG 2 cut(s) 936, 2365
AvaII GGWCC 4 cut(s) 362, 731, 1290, 3734
BaeGI GKGCMC 3 cut(s) 1413, 1596, 3752
BaeI ACNNNNGTAYC 2 cut(s) 642, 675
BalI TGGCCA 1 cut(s) 456
BamHI GGATCC 1 cut(s) 556
BanI GGYRCC 2 cut(s) 437, 3749
BanII GRGCYC 2 cut(s) 57, 3218
BarI GAAGNNNNNNTAC 6 cut(s) 825, 857, 3682, 3714, 3804, 3836
BauI CACGAG 2 cut(s) 2507, 3143
BbrPI CACGTG 1 cut(s) 919
BbsI GAAGAC 2 cut(s) 1166, 2985
Bbv12I GWGCWC 5 cut(s) 57, 630, 903, 1413, 1596
BcgI CGANNNNNNTGC 2 cut(s) 1221, 1255
BciT130I CCWGG 9 cut(s) 536, 1117, 1298, 1548, 2247, 2577, 2932, 3371, 3792
BclI TGATCA 4 cut(s) 1257, 3423, 3438, 3483
BcnI CCSGG 2 cut(s) 339, 3635
BcoDI GTCTC 6 cut(s) 286, 866, 1134, 1616, 2492, 3713
BcuI ACTAGT 2 cut(s) 266, 2533
BfmI CTRYAG 3 cut(s) 711, 837, 2037
BfuAI ACCTGC 3 cut(s) 871, 1301, 3745
BglI GCCNNNNNGGC 1 cut(s) 1738
BmcAI AGTACT 3 cut(s) 843, 1046, 3049
Bme18I GGWCC 4 cut(s) 362, 731, 1290, 3734
BmeT110I CYCGRG 2 cut(s) 936, 2365
BmgT120I GGNCC 9 cut(s) 362, 731, 932, 1290, 1746, 3367, 3373, 3734, 3788
BmiI GGNNCC 5 cut(s) 150, 364, 439, 558, 3751
BmrI ACTGGG 5 cut(s) 1057, 1457, 2090, 3221, 3662
BmsI GCATC 3 cut(s) 593, 1637, 2925
BmuI ACTGGG 5 cut(s) 1057, 1457, 2090, 3221, 3662
BpiI GAAGAC 2 cut(s) 1166, 2985
BplI GAGNNNNNCTC 2 cut(s) 3037, 3069
BpmI CTGGAG 2 cut(s) 2103, 2960
Bpu14I TTCGAA 1 cut(s) 1061
BpuEI CTTGAG 2 cut(s) 47, 2415
BpuMI CCSGG 2 cut(s) 339, 3635
BsaAI YACGTR 1 cut(s) 919
BsaHI GRCGYC 3 cut(s) 8, 24, 2304
BsaI GGTCTC 3 cut(s) 1134, 1616, 3713
BsaJI CCNNGG 8 cut(s) 534, 1546, 1733, 3369, 3370, 3778, 3790, 3791
BsaWI WCCGGW 2 cut(s) 27, 1617
BsaXI ACNNNNNCTCC 8 cut(s) 1103, 1133, 2168, 2198, 3647, 3677, 3795, 3825
Bse3DI GCAATG 5 cut(s) 519, 1650, 1748, 1934, 3393
BseAI TCCGGA 2 cut(s) 27, 1617
BseBI CCWGG 9 cut(s) 536, 1117, 1298, 1548, 2247, 2577, 2932, 3371, 3792
BseDI CCNNGG 8 cut(s) 534, 1546, 1733, 3369, 3370, 3778, 3790, 3791
BseGI GGATG 9 cut(s) 760, 1141, 1495, 2668, 2940, 3235, 3630, 3801, 3834
BseMI GCAATG 5 cut(s) 519, 1650, 1748, 1934, 3393
BseMII CTCAG 5 cut(s) 660, 861, 1055, 1469, 3697
BseRI GAGGAG 4 cut(s) 95, 2418, 2459, 3042
BseSI GKGCMC 3 cut(s) 1413, 1596, 3752
BseYI CCCAGC 1 cut(s) 1553
BsgI GTGCAG 3 cut(s) 849, 2737, 3422
Bsh1236I CGCG 3 cut(s) 51, 1243, 1782
BshNI GGYRCC 2 cut(s) 437, 3749
BsiHKAI GWGCWC 5 cut(s) 57, 630, 903, 1413, 1596
BsiHKCI CYCGRG 2 cut(s) 936, 2365
BsiSI CCGG 6 cut(s) 28, 111, 339, 554, 1618, 3635
BslFI GGGAC 3 cut(s) 705, 2041, 3602
BsmAI GTCTC 6 cut(s) 286, 866, 1134, 1616, 2492, 3713
BsmBI CGTCTC 1 cut(s) 286
BsmFI GGGAC 3 cut(s) 705, 2041, 3602
BsmI GAATGC 1 cut(s) 1429
Bso31I GGTCTC 3 cut(s) 1134, 1616, 3713
BsoBI CYCGRG 2 cut(s) 936, 2365
Bsp119I TTCGAA 1 cut(s) 1061
Bsp1286I GDGCHC 7 cut(s) 57, 630, 903, 1413, 1596, 3218, 3752
Bsp13I TCCGGA 2 cut(s) 27, 1617
Bsp19I CCATGG 1 cut(s) 1733
Bsp68I TCGCGA 1 cut(s) 1243
BspACI CCGC 9 cut(s) 5, 49, 324, 418, 1001, 2232, 2913, 3365, 3527
BspCNI CTCAG 5 cut(s) 659, 862, 1054, 1468, 3696
BspEI TCCGGA 2 cut(s) 27, 1617
BspFNI CGCG 3 cut(s) 51, 1243, 1782
BspHI TCATGA 2 cut(s) 393, 3435
BspLI GGNNCC 5 cut(s) 150, 364, 439, 558, 3751
BspMAI CTGCAG 1 cut(s) 2041
BspMI ACCTGC 3 cut(s) 871, 1301, 3745
BspPI GGATC 7 cut(s) 214, 551, 564, 1041, 1675, 1826, 3379
BspQI GCTCTTC 1 cut(s) 2475
BspT104I TTCGAA 1 cut(s) 1061
BspT107I GGYRCC 2 cut(s) 437, 3749
BspTNI GGTCTC 3 cut(s) 1134, 1616, 3713
BsrBI CCGCTC 1 cut(s) 2232
BsrDI GCAATG 5 cut(s) 519, 1650, 1748, 1934, 3393
BssECI CCNNGG 8 cut(s) 534, 1546, 1733, 3369, 3370, 3778, 3790, 3791
BssNAI GTATAC 1 cut(s) 3415
BssNI GRCGYC 3 cut(s) 8, 24, 2304
BssSI CACGAG 2 cut(s) 2507, 3143
BssT1I CCWWGG 2 cut(s) 1733, 3778
Bst1107I GTATAC 1 cut(s) 3415
Bst2BI CACGAG 2 cut(s) 2507, 3143
Bst2UI CCWGG 9 cut(s) 536, 1117, 1298, 1548, 2247, 2577, 2932, 3371, 3792
Bst6I CTCTTC 3 cut(s) 1619, 2475, 3021
BstACI GRCGYC 3 cut(s) 8, 24, 2304
BstAPI GCANNNNNTGC 1 cut(s) 1634
BstBAI YACGTR 1 cut(s) 919
BstBI TTCGAA 1 cut(s) 1061
BstC8I GCNNGC 5 cut(s) 53, 1234, 1265, 2559, 2852
BstDEI CTNAG 7 cut(s) 646, 870, 1041, 1455, 1985, 2552, 3683
BstDSI CCRYGG 1 cut(s) 1733
BstEII GGTNACC 2 cut(s) 2360, 2944
BstENI CCTNNNNNAGG 1 cut(s) 2480
BstF5I GGATG 9 cut(s) 760, 1141, 1495, 2668, 2940, 3235, 3630, 3801, 3834
BstFNI CGCG 3 cut(s) 51, 1243, 1782
BstHHI GCGC 1 cut(s) 1784
BstMAI GTCTC 6 cut(s) 286, 866, 1134, 1616, 2492, 3713
BstNI CCWGG 9 cut(s) 536, 1117, 1298, 1548, 2247, 2577, 2932, 3371, 3792
BstNSI RCATGY 3 cut(s) 1236, 1326, 2561
BstPI GGTNACC 2 cut(s) 2360, 2944
BstSFI CTRYAG 3 cut(s) 711, 837, 2037
BstSLI GKGCMC 3 cut(s) 1413, 1596, 3752
BstUI CGCG 3 cut(s) 51, 1243, 1782
BstV2I GAAGAC 2 cut(s) 1166, 2985
BstX2I RGATCY 2 cut(s) 556, 3384
BstXI CCANNNNNNTGG 3 cut(s) 2299, 2931, 3675
BstYI RGATCY 2 cut(s) 556, 3384
BstZ17I GTATAC 1 cut(s) 3415
BtgI CCRYGG 1 cut(s) 1733
BtgZI GCGATG 1 cut(s) 3188
BtsCI GGATG 9 cut(s) 760, 1141, 1495, 2668, 2940, 3235, 3630, 3801, 3834
BtsIMutI CAGTG 4 cut(s) 78, 1959, 2007, 3554
BtuMI TCGCGA 1 cut(s) 1243
BveI ACCTGC 3 cut(s) 871, 1301, 3745
Cac8I GCNNGC 5 cut(s) 53, 1234, 1265, 2559, 2852
CaiI CAGNNNCTG 1 cut(s) 3815
CciI TCATGA 2 cut(s) 393, 3435
CfoI GCGC 1 cut(s) 1784
Cfr13I GGNCC 9 cut(s) 362, 731, 932, 1290, 1746, 3367, 3373, 3734, 3788
CseI GACGC 1 cut(s) 2693
CsiI ACCWGGT 1 cut(s) 2245
Csp6I GTAC 9 cut(s) 164, 842, 1045, 1972, 1993, 2379, 2917, 3013, 3048
CviQI GTAC 9 cut(s) 164, 842, 1045, 1972, 1993, 2379, 2917, 3013, 3048
DdeI CTNAG 7 cut(s) 646, 870, 1041, 1455, 1985, 2552, 3683
EaeI YGGCCR 1 cut(s) 454
Eam1104I CTCTTC 3 cut(s) 1619, 2475, 3021
EarI CTCTTC 3 cut(s) 1619, 2475, 3021
Ecl136II GAGCTC 1 cut(s) 55
Eco130I CCWWGG 2 cut(s) 1733, 3778
Eco24I GRGCYC 2 cut(s) 57, 3218
Eco31I GGTCTC 3 cut(s) 1134, 1616, 3713
Eco32I GATATC 2 cut(s) 1452, 2355
Eco47I GGWCC 4 cut(s) 362, 731, 1290, 3734
Eco53kI GAGCTC 1 cut(s) 55
Eco57I CTGAAG 1 cut(s) 3264
Eco72I CACGTG 1 cut(s) 919
Eco88I CYCGRG 2 cut(s) 936, 2365
Eco91I GGTNACC 2 cut(s) 2360, 2944
EcoICRI GAGCTC 1 cut(s) 55
EcoNI CCTNNNNNAGG 1 cut(s) 2480
EcoO109I RGGNCCY 4 cut(s) 362, 932, 1290, 3373
EcoO65I GGTNACC 2 cut(s) 2360, 2944
EcoRI GAATTC 1 cut(s) 3001
EcoRII CCWGG 9 cut(s) 534, 1115, 1296, 1546, 2245, 2575, 2930, 3369, 3790
EcoRV GATATC 2 cut(s) 1452, 2355
EcoT14I CCWWGG 2 cut(s) 1733, 3778
EcoT22I ATGCAT 1 cut(s) 1936
EcoT38I GRGCYC 2 cut(s) 57, 3218
ErhI CCWWGG 2 cut(s) 1733, 3778
Esp3I CGTCTC 1 cut(s) 286
FalI AAGNNNNNCTT 8 cut(s) 334, 366, 2545, 2577, 3737, 3769, 3762, 3794
FaqI GGGAC 3 cut(s) 705, 2041, 3602
FauI CCCGC 1 cut(s) 2906
FauNDI CATATG 1 cut(s) 1771
FbaI TGATCA 4 cut(s) 1257, 3423, 3438, 3483
FblI GTMKAC 1 cut(s) 3414
FokI GGATG 9 cut(s) 767, 1148, 1482, 2675, 2947, 3242, 3617, 3808, 3821
FriOI GRGCYC 2 cut(s) 57, 3218
GlaI GCGC 1 cut(s) 1783
GsaI CCCAGC 1 cut(s) 1557
GsuI CTGGAG 2 cut(s) 2103, 2960
HapII CCGG 6 cut(s) 28, 111, 339, 554, 1618, 3635
HgaI GACGC 1 cut(s) 2693
HhaI GCGC 1 cut(s) 1784
Hin1I GRCGYC 3 cut(s) 8, 24, 2304
Hin6I GCGC 1 cut(s) 1782
HinP1I GCGC 1 cut(s) 1782
HindIII AAGCTT 1 cut(s) 461
HpaII CCGG 6 cut(s) 28, 111, 339, 554, 1618, 3635
HphI GGTGA 8 cut(s) 523, 728, 1752, 1901, 2235, 2239, 3433, 3722
Hpy166II GTNNAC 5 cut(s) 1411, 1594, 2164, 3161, 3415
Hpy8I GTNNAC 5 cut(s) 1411, 1594, 2164, 3161, 3415
Hpy99I CGWCG 2 cut(s) 13, 26
HpyCH4IV ACGT 7 cut(s) 24, 195, 488, 918, 1970, 2304, 2377
HpyF3I CTNAG 7 cut(s) 646, 870, 1041, 1455, 1985, 2552, 3683
HpySE526I ACGT 7 cut(s) 24, 195, 488, 918, 1970, 2304, 2377
Hsp92I GRCGYC 3 cut(s) 8, 24, 2304
HspAI GCGC 1 cut(s) 1782
Kpn2I TCCGGA 2 cut(s) 27, 1617
Ksp22I TGATCA 4 cut(s) 1257, 3423, 3438, 3483
LguI GCTCTTC 1 cut(s) 2475
LmnI GCTCC 6 cut(s) 906, 1156, 1219, 1464, 1834, 2279
LweI GCATC 3 cut(s) 593, 1637, 2925
MabI ACCWGGT 1 cut(s) 2245
MaeII ACGT 7 cut(s) 24, 195, 488, 918, 1970, 2304, 2377
MbiI CCGCTC 1 cut(s) 2232
MfeI CAATTG 3 cut(s) 654, 1848, 1945
MflI RGATCY 2 cut(s) 556, 3384
MhlI GDGCHC 7 cut(s) 57, 630, 903, 1413, 1596, 3218, 3752
MlsI TGGCCA 1 cut(s) 456
MluNI TGGCCA 1 cut(s) 456
MlyI GAGTC 8 cut(s) 971, 1121, 1951, 2023, 2458, 2499, 3038, 3056
MmeI TCCRAC 7 cut(s) 176, 640, 709, 930, 1291, 3080, 3712
Mox20I TGGCCA 1 cut(s) 456
Mph1103I ATGCAT 1 cut(s) 1936
MroI TCCGGA 2 cut(s) 27, 1617
MroXI GAANNNNTTC 3 cut(s) 1562, 3343, 3599
MscI TGGCCA 1 cut(s) 456
MslI CAYNNNNRTG 3 cut(s) 2297, 2705, 3398
Msp20I TGGCCA 1 cut(s) 456
MspA1I CMGCKG 6 cut(s) 324, 1673, 2081, 3335, 3365, 3563
MspI CCGG 6 cut(s) 28, 111, 339, 554, 1618, 3635
MunI CAATTG 3 cut(s) 654, 1848, 1945
Mva1269I GAATGC 1 cut(s) 1429
MvaI CCWGG 9 cut(s) 536, 1117, 1298, 1548, 2247, 2577, 2932, 3371, 3792
MvnI CGCG 3 cut(s) 51, 1243, 1782
NciI CCSGG 2 cut(s) 339, 3635
NcoI CCATGG 1 cut(s) 1733
NdeI CATATG 1 cut(s) 1771
NlaIV GGNNCC 5 cut(s) 150, 364, 439, 558, 3751
NmeAIII GCCGAG 1 cut(s) 3197
NmuCI GTSAC 5 cut(s) 914, 1318, 1954, 2184, 3538
NruI TCGCGA 1 cut(s) 1243
NsiI ATGCAT 1 cut(s) 1936
NspI RCATGY 3 cut(s) 1236, 1326, 2561
NspV TTCGAA 1 cut(s) 1061
PaeI GCATGC 2 cut(s) 1236, 2561
PaeR7I CTCGAG 1 cut(s) 936
PagI TCATGA 2 cut(s) 393, 3435
PasI CCCWGGG 2 cut(s) 3370, 3791
PciSI GCTCTTC 1 cut(s) 2475
PcsI WCGNNNNNNNCGW 3 cut(s) 81, 192, 2514
PctI GAATGC 1 cut(s) 1429
PdmI GAANNNNTTC 3 cut(s) 1562, 3343, 3599
PfeI GAWTC 9 cut(s) 31, 397, 972, 1704, 2201, 2895, 2905, 3139, 3595
PflMI CCANNNNNTGG 1 cut(s) 374
PleI GAGTC 8 cut(s) 970, 1120, 1951, 2023, 2457, 2499, 3037, 3056
PmaCI CACGTG 1 cut(s) 919
PmlI CACGTG 1 cut(s) 919
PpsI GAGTC 8 cut(s) 970, 1120, 1951, 2023, 2457, 2499, 3037, 3056
Ppu21I YACGTR 1 cut(s) 919
PpuMI RGGWCCY 2 cut(s) 362, 1290
PshBI ATTAAT 1 cut(s) 2889
Psp124BI GAGCTC 1 cut(s) 57
Psp5II RGGWCCY 2 cut(s) 362, 1290
Psp6I CCWGG 9 cut(s) 534, 1115, 1296, 1546, 2245, 2575, 2930, 3369, 3790
PspCI CACGTG 1 cut(s) 919
PspEI GGTNACC 2 cut(s) 2360, 2944
PspFI CCCAGC 1 cut(s) 1553
PspGI CCWGG 9 cut(s) 534, 1115, 1296, 1546, 2245, 2575, 2930, 3369, 3790
PspN4I GGNNCC 5 cut(s) 150, 364, 439, 558, 3751
PspPI GGNCC 9 cut(s) 362, 731, 932, 1290, 1746, 3367, 3373, 3734, 3788
PspPPI RGGWCCY 2 cut(s) 362, 1290
PspXI VCTCGAGB 1 cut(s) 936
PsrI GAACNNNNNNTAC 2 cut(s) 1863, 1895
PstI CTGCAG 1 cut(s) 2041
PstNI CAGNNNCTG 1 cut(s) 3815
PsuI RGATCY 2 cut(s) 556, 3384
PvuII CAGCTG 4 cut(s) 1673, 2081, 3335, 3563
RruI TCGCGA 1 cut(s) 1243
RsaI GTAC 9 cut(s) 165, 843, 1046, 1973, 1994, 2380, 2918, 3014, 3049
RsaNI GTAC 9 cut(s) 164, 842, 1045, 1972, 1993, 2379, 2917, 3013, 3048
RseI CAYNNNNRTG 3 cut(s) 2297, 2705, 3398
SacI GAGCTC 1 cut(s) 57
SapI GCTCTTC 1 cut(s) 2475
Sau96I GGNCC 9 cut(s) 362, 731, 932, 1290, 1746, 3367, 3373, 3734, 3788
ScaI AGTACT 3 cut(s) 843, 1046, 3049
SchI GAGTC 8 cut(s) 971, 1121, 1951, 2023, 2458, 2499, 3038, 3056
SduI GDGCHC 7 cut(s) 57, 630, 903, 1413, 1596, 3218, 3752
SexAI ACCWGGT 1 cut(s) 2245
SfaNI GCATC 3 cut(s) 593, 1637, 2925
SfcI CTRYAG 3 cut(s) 711, 837, 2037
Sfr274I CTCGAG 1 cut(s) 936
SfuI TTCGAA 1 cut(s) 1061
SinI GGWCC 4 cut(s) 362, 731, 1290, 3734
SlaI CTCGAG 1 cut(s) 936
SmiMI CAYNNNNRTG 3 cut(s) 2297, 2705, 3398
SmlI CTYRAG 3 cut(s) 62, 936, 2430
SmoI CTYRAG 3 cut(s) 62, 936, 2430
SpeI ACTAGT 2 cut(s) 266, 2533
SphI GCATGC 2 cut(s) 1236, 2561
SsiI CCGC 9 cut(s) 5, 49, 324, 418, 1001, 2232, 2913, 3365, 3527
SspI AATATT 1 cut(s) 565
SstI GAGCTC 1 cut(s) 57
StyI CCWWGG 2 cut(s) 1733, 3778
TaiI ACGT 7 cut(s) 27, 198, 491, 921, 1973, 2307, 2380
TaqII GACCGA 1 cut(s) 620
TatI WGTACW 4 cut(s) 841, 1044, 3012, 3047
TauI GCSGC 4 cut(s) 8, 327, 3368, 3530
TfiI GAWTC 9 cut(s) 31, 397, 972, 1704, 2201, 2895, 2905, 3139, 3595
TscAI CASTG 4 cut(s) 85, 1959, 2007, 3561
TseFI GTSAC 5 cut(s) 914, 1318, 1954, 2184, 3538
Tsp45I GTSAC 5 cut(s) 914, 1318, 1954, 2184, 3538
TspGWI ACGGA 2 cut(s) 1556, 3074
TspRI CASTG 4 cut(s) 85, 1959, 2007, 3561
Van91I CCANNNNNTGG 1 cut(s) 374
VneI GTGCAC 2 cut(s) 1409, 1592
VpaK11BI GGWCC 4 cut(s) 362, 731, 1290, 3734
VspI ATTAAT 1 cut(s) 2889
XagI CCTNNNNNAGG 1 cut(s) 2480
XapI RAATTY 7 cut(s) 600, 814, 1559, 1799, 3001, 3464, 3869
XceI RCATGY 3 cut(s) 1236, 1326, 2561
XcmI CCANNNNNNNNNTGG 2 cut(s) 1740, 2677
XhoI CTCGAG 1 cut(s) 936
XmiI GTMKAC 1 cut(s) 3414
XmnI GAANNNNTTC 3 cut(s) 1562, 3343, 3599
ZraI GACGTC 2 cut(s) 25, 2305
ZrmI AGTACT 3 cut(s) 843, 1046, 3049
Zsp2I ATGCAT 1 cut(s) 1936
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.