Rw5G042400
ERF Family

Belongs to the ABC transporter superfamily. ABCG family. PDR (TC 3.A.1.205) subfamily

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr5
OG0007618
Physical Location & Seq
Forward (+)
74304984 .. 74312937
7954 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw5G042400.1

Sequence Viewer

Length: 4182 bp
ATGGCGGCATCGAACGGGAGCGAGTACTTCGAATTGGGAATCGAGGGGCCGAACGAGACTTTCGCTCGGCCGTCGAACGCCGAGTCGGTGGCGGAGGACGAGGAGGAGCTGATGTGGGCGGCGATCAAGCGGCTGCCGTCGCAGAAGCAGTCGAGCACCGCCATTCTCAAGTCCGACAAGTCGGAGACCATCGACGTCCGGAAACTCGATCGCCACAACCGCGAGCTCGTCGTCAGCAAAGCCCTCGCCACTAACGATCAGGACAACCACCGTCTCCTCTGCGCCATTAAAGAACGCCTTGACAGAGTGGGATTGGAGGTTCGAAAAGTGGAAGTGCGGTACGAGAACCTGAATATCGTGGCAGACGTTCAAACAGGATCAAGAGCTTTGCCTACTCTCATTAACTATACTCGTGATGGTATAGAGGGTTTTTTAACTAGTTGTAGGATTTTTAAACCCAAGAGACGGTCTTTAAAGATTTTGAACAACGTCAGCGGTGTTATCAAACCCGGAAGGATGACTTTGCTTTTAGGACCCCCAAGTTCTGGCAAAACCACTTTGCTCACAACTCTTGCAGGAAAACTGGACTCCAGCTTAAAGAAAAGTGGTAGCATTACCTACAATGGGCATAAGCTTGATGAGTTCTGCGTTCAAAGAACTGCTGCCTACATAAGCCAAACAGATAATCACATTGCAGAACTCACCGTGAGAGAAACCTTAGACTTTGCAGCTAGATGTCAAGGTGCAAGCGAAGGTTTTGCAGCGTACATGAAAAATCTGGTCAAGTTAGAGAAGGAAAAAGACATAAGGCCAGATCCAGTAATTGATGCATTCATGAAGGCATCATCTGTTGGTGGTAAAAAGCACAGTATTACAACAGATTATGTTCTCAAAGTGCTTGGTCTTGATGTTTGTTCAGAGACAATTGTTGGAAACGACATGTTAAGAGGGGTTTCTGGGGGACAAAGGAAAAGGGTTACTACAGGTGAAATGGCAGTTGGACCAAGAAAAACCCTGTTTATGGATGAAATATCTACTGGATTGGATAGTTCTACTACATACCAAATTGTAAAATGTATTGGGAATTTTGTTCATCACATGGAAGCTACAGTACTAATGGCTCTGCTTCAGCCTGCACCTGAGACATTTGAATTGTTTGATGATTTAGTGCTACTATCTGAAGGCTACGTGGTTTATCAAGGCCCTCGAGCAGAAGTGTTGGAGTTCTTCGAGTCATTAGGCTTCAAACTACCACCACGAAAGGGTGTTGCAGATTTTCTTCAAGAGGTAACGTCTAAAAAAGATCAAGCTCAGTACTGGGCTGATCAATTGAAACCGTACGCATACATTTCTGTTTCAGAAATTGCAGAAGCCTTTAAGAAGTCCAGGTTTGGAAGGTCTGTGGATGACACTCTCTCTGCTCCATATGATAAATCTAGCAGTCTTCCTGCGGCTTTGCCAAAAACTAAATATGCAACCTCAAAATGGGAGCTTTGTAAAGCATGCTTTTCACGAGAACTACTGTTGATCAGCAGGCATAGGTTTCTTTACACATTTAGGACCTGCCAGGTTGCATTTGTTGGGGCTGTGACTTGTACAATGTTTTCTAGATTGAAATTACACCCGACAGATGAAATAAATGGCAACCTTTATCTTTCTTGCTTGTTCTTTGGGTTGGTGCACATGATGTTTAATGGGTTTTCCGAGCTACCTCTTATGATATCTCGGCTTCCAGTCTTTTACAAGCAAAGAGACAATCACTTTCATCCTGCATGGGCATGGTCTGTTGTTAGTTTGCTTCTATTTTTCCGTTTCATGTTTTTGCTGTTCTCTGTGCACCAAATGGCTTTGGGTCTCTTCCGGACAATGGCATCTCTTGCAAGAGATATGGTCATTGCCAATACATTTGGATCAGCTGCACTAATTATTATATTTTCTTTTGGTGGATTCATCATTCCCAAAGCTAGCATTAAGCCATGGTGGGAATGGGCCTTTTGGATATCACCACTATCATATGGACAACGTGCTCTTTCTGTCAATGAATTTACGGCTGTAAGGTGGATCAAGATATCTCCTCTAACTAACAGAACAATTGGGCAAAGTGTTCTCCAAGAACACAACTTACCAAATGGTGATTACTGGTATTGGATTGGAGTTGGTGCTCTGTTAGCTTATGCATTGCTTTTCAATTGCATAGTGACTCTGACCTTGATGTACCTAAATCCACTAAGGAAAAATCAGACAGTGCTTCCACTTGATGACACACAAGAGAGTCCTGCTGCAGATGGGGGTAAATATAAGACCGAGTCTATCCCAAAATCAACTGGAGATAACGGCCCAAGAAAGGGAATGATCTTACCATTTCAGCCATTAACAATGACTTTCCACAATGTTAACTACTCTGTTGACATGCCAAAGGAAATGAAGTCACAAGGTATACCAGAATCGAAGTTGAAACTCTTGTCGGAGGTGAGTGGAGTGTTCTCACCAGGTGTTCTTACAGCTTTAGTTGGGTCTAGTGGAGCTGGAAAGACCACTTTGATGGACGTCCTTGCTGGTAGGAAAACTGGTGGATACATAGAAGGAGATATCAAGATATCTGGTTACCCGAAAGAGCAACGTACTTTTGCAAGAATAGCAGGATATGTTGAGCAAAATGACATACATTCTCCTCAACTTACAGTTGAAGAGTCTCTATGGTTTTCTTCTGCTCTTCGCCTTCCGAAGGAAGTCAGCACAGAGAAAAGACTTGAGTTTGTCGAAGAAGTAATGAAACTAGTAGAGCTTGATACTCTAAGGCATGCTTTGGTTGGTTTGCCAGGCAGTTCAGGCTTATCAACAGAGCAAAGAAAACGCTTAACAATTTCTGTTGAGCTTGTTGCCAACCCTTCAATTATCTTTATGGATGAACCCACATCTGGACTTGATGCAAGGGCAGCAGCCATTGTGATGAGGACTGTTCGTAATACAGTTGATACAGGAAGAACGGTGGTCTGCACTATACATCAACCAAGTATTGACATATTTGAGGCATTTGATGAGCTTCTTCTTATGAAACGAGGGGGGCAAGTTATTTATGGAGGAAACCTAGGCCAGCACTCCCAGACAATGATAAACTACTTTCAGGAGATCAATGGAATCACTCCGATTCCAAGCGGGTACAATCCAGCAACCTGGATGCTGGAGGTAACCACACCGGCTTGTGAACAGAGAATTGGGGAAGACTTTGCTAGCATATACAGAAAATCAGAGCAGTACAGGGAGGTGGAAGAGTCCATCAAGCAGTTTAGCACTCCTCCAGCTGACTCAAAACCATTAGAGTTTGCTTCCACGTATTCACAAAACACATTGGCTCAATTTTGGACCTGCTTCTGGAAACAAAATCTTGTGTATTGGAGAAGTCCACAATATAATGCCATGAGGTTAATCTTTACTACGCTTGCTGCATTGATATTCGGTTCAGCATTTTGGGATGTTGGTACAAAAAGGGACTCAATTCAATCATTGATGATGGTTATGGGAGCTCTTTATTCTGCTTGCCTGTTTCTTGGGGTCAATAACGCTTCTTCAGTTCAGCCAATTGTTTCAATTGAGAGGACAGTATTTTATCGAGAGAAAGCAGCTGGAATGTATTCTCCTTTCGCTTATGCAGCGGCCCAGGGCCTTATAGAGATCCCATACATTGCTGTGCAGACAATAGTATACGGGGTAATCACATACTTCATGGTCAATTTTGAAAGGACAATCACAAAATTCTTGCTCTATATTCTGTTCATGTTCCTCACATTCACCTACTTCACCCTTTATGGCATGGCTGCTGTTGGTCTCACTCCCTCTCAACACCTGGCAGCTGTCATCTCTTCTGCATTTTACTCATTGTGGAATCTCCTCGCCGGTTTCCTCATCCCCAAACCAAACATCCCGCCATGGTGGATATGGTTTTACTACATCACCCCAGTTGCATGGACACTTAGAGGTATTATCACTTCCCAACTTGGTGATGTGGAGACCATTCTTGTCGGACCGGCGTTTAAGGGCACTGTGAAAGAATACTTGGAAGTCAGCCTTGGCTATGGCCCTGGAATGATTGGAGTTTCAGTAGCTGTGCTACTGGGTTTCAACGTCCTTTTCTTCAGTATCTTTGCCTTCTCCATCAAATTTCTTAACTTCCAGAGAAGATAG

Protein Analysis

1393

Amino Acids

155.85

Weight (kDa)

8.64

Isoelectric Point (pI)

34.98

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
ABC_trans_N PF14510 85 - 135 2.2e-06 ABC-transporter N-terminal
ABC_tran PF00005 161 - 343 2.4e-16 ABC transporter
ABC2_membrane_7 PF19055 377 - 427 4.2e-06 ABC-2 type transporter
ABC2_membrane PF01061 498 - 601 1.3e-13 ABC-2 type transporter
ABC2_membrane PF01061 602 - 682 2.4e-18 ABC-2 type transporter
PDR_assoc PF08370 687 - 749 9.6e-23 Plant PDR ABC transporter associated
ABC_tran PF00005 822 - 973 6.3e-20 ABC transporter
ABC2_membrane PF01061 1119 - 1331 2.3e-57 ABC-2 type transporter
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 2 cut(s) 198, 2550
Acc36I ACCTGC 2 cut(s) 1571, 3374
AccB7I CCANNNNNTGG 1 cut(s) 545
AccI GTMKAC 2 cut(s) 2437, 3702
AccII CGCG 1 cut(s) 222
AccIII TCCGGA 2 cut(s) 198, 1860
AclWI GGATC 5 cut(s) 385, 809, 1918, 2069, 3667
AcoI YGGCCR 1 cut(s) 68
AcsI RAATTY 4 cut(s) 1084, 2042, 3752, 4157
AcuI CTGAAG 4 cut(s) 1112, 1200, 3552, 4117
AcyI GRCGYC 2 cut(s) 195, 2547
AdeI CACNNNGTG 1 cut(s) 2492
AflIII ACRYGT 1 cut(s) 939
AhlI ACTAGT 2 cut(s) 437, 2776
AjnI CCWGG 8 cut(s) 1385, 1566, 2488, 2818, 3173, 3657, 3843, 4078
AjuI GAANNNNNNNTTGG 8 cut(s) 981, 1013, 3198, 3230, 4037, 4050, 4069, 4082
AloI GAACNNNNNNTCC 2 cut(s) 303, 335
Alw21I GWGCWC 7 cut(s) 158, 228, 1683, 1839, 2029, 2164, 3526
Alw44I GTGCAC 2 cut(s) 1679, 1835
AlwI GGATC 5 cut(s) 385, 809, 1918, 2069, 3667
AlwNI CAGNNNCTG 1 cut(s) 4103
Ama87I CYCGRG 1 cut(s) 1206
Aor13HI TCCGGA 2 cut(s) 198, 1860
ApaLI GTGCAC 2 cut(s) 1679, 1835
ApoI RAATTY 4 cut(s) 1084, 2042, 3752, 4157
ArsI GACNNNNNNTTYG 2 cut(s) 68, 100
Asp700I GAANNNNTTC 1 cut(s) 3631
AspA2I CCTAGG 1 cut(s) 3088
AspLEI GCGC 1 cut(s) 284
AsuC2I CCSGG 1 cut(s) 510
AsuII TTCGAA 2 cut(s) 30, 322
AsuNHI GCTAGC 2 cut(s) 1964, 3230
AvaI CYCGRG 1 cut(s) 1206
AvaII GGWCC 5 cut(s) 533, 1001, 1560, 3363, 4022
AvrII CCTAGG 1 cut(s) 3088
BaeGI GKGCMC 3 cut(s) 1683, 1839, 4040
BanII GRGCYC 2 cut(s) 228, 3526
BarI GAAGNNNNNNTAC 4 cut(s) 1095, 1127, 3970, 4002
BauI CACGAG 2 cut(s) 411, 1512
BbsI GAAGAC 2 cut(s) 1436, 3228
Bbv12I GWGCWC 7 cut(s) 158, 228, 1683, 1839, 2029, 2164, 3526
BccI CCATC 7 cut(s) 197, 410, 2279, 2536, 3284, 3505, 4160
BceAI ACGGC 4 cut(s) 55, 121, 2064, 2350
BcgI CGANNNNNNTGC 4 cut(s) 226, 260, 740, 774
BciT130I CCWGG 8 cut(s) 1387, 1568, 2490, 2820, 3175, 3659, 3845, 4080
BciVI GTATCC 1 cut(s) 2567
BclI TGATCA 2 cut(s) 1324, 1527
BcnI CCSGG 1 cut(s) 510
BcuI ACTAGT 2 cut(s) 437, 2776
BfaI CTAG 9 cut(s) 438, 732, 1437, 1608, 1965, 2517, 2777, 3089, 3231
BfmI CTRYAG 3 cut(s) 981, 1107, 2280
BfuAI ACCTGC 2 cut(s) 1571, 3374
BfuI GTATCC 1 cut(s) 2567
BlnI CCTAGG 1 cut(s) 3088
BmcAI AGTACT 3 cut(s) 26, 1113, 1316
Bme1390I CCNGG 9 cut(s) 510, 1387, 1568, 2490, 2820, 3175, 3659, 3845, 4080
Bme18I GGWCC 5 cut(s) 533, 1001, 1560, 3363, 4022
BmeT110I CYCGRG 1 cut(s) 1206
BmiI GGNNCC 2 cut(s) 48, 535
BmrFI CCNGG 9 cut(s) 510, 1387, 1568, 2490, 2820, 3175, 3659, 3845, 4080
BmrI ACTGGG 3 cut(s) 1327, 3950, 4121
BmsI GCATC 6 cut(s) 17, 817, 851, 1880, 2917, 3168
BmtI GCTAGC 2 cut(s) 1968, 3234
BmuI ACTGGG 3 cut(s) 1327, 3950, 4121
BpiI GAAGAC 2 cut(s) 1436, 3228
BpmI CTGGAG 4 cut(s) 574, 2346, 3203, 3282
Bpu14I TTCGAA 2 cut(s) 30, 322
BpuEI CTTGAG 2 cut(s) 152, 2771
BpuMI CCSGG 1 cut(s) 510
BsaAI YACGTR 2 cut(s) 1189, 3333
BsaHI GRCGYC 2 cut(s) 195, 2547
BsaI GGTCTC 4 cut(s) 179, 1859, 3830, 4001
BsaJI CCNNGG 7 cut(s) 1976, 3088, 3657, 3658, 3926, 4066, 4078
BsaWI WCCGGW 2 cut(s) 198, 1860
BsaXI ACNNNNNCTCC 4 cut(s) 98, 128, 4083, 4113
Bse118I RCCGGY 3 cut(s) 3196, 3893, 4024
Bse3DI GCAATG 4 cut(s) 690, 1893, 2177, 3681
BseAI TCCGGA 2 cut(s) 198, 1860
BseBI CCWGG 8 cut(s) 1387, 1568, 2490, 2820, 3175, 3659, 3845, 4080
BseDI CCNNGG 7 cut(s) 1976, 3088, 3657, 3658, 3926, 4066, 4078
BseGI GGATG 9 cut(s) 522, 1030, 1411, 1765, 2911, 3183, 3478, 3903, 3918
BseMI GCAATG 4 cut(s) 690, 1893, 2177, 3681
BseMII CTCAG 2 cut(s) 1131, 1325
BseRI GAGGAG 7 cut(s) 116, 119, 266, 2064, 2661, 3285, 3878
BseSI GKGCMC 3 cut(s) 1683, 1839, 4040
BseX3I CGGCCG 1 cut(s) 68
BsgI GTGCAG 4 cut(s) 1119, 1902, 2980, 3710
Bsh1236I CGCG 1 cut(s) 222
Bsh1285I CGRYCG 2 cut(s) 71, 211
BsiEI CGRYCG 2 cut(s) 71, 211
BsiHKAI GWGCWC 7 cut(s) 158, 228, 1683, 1839, 2029, 2164, 3526
BsiHKCI CYCGRG 1 cut(s) 1206
BsiSI CCGG 6 cut(s) 199, 510, 1861, 3197, 3894, 4025
BsiWI CGTACG 1 cut(s) 1338
BslFI GGGAC 2 cut(s) 975, 3503
BsmBI CGTCTC 2 cut(s) 278, 457
BsmFI GGGAC 2 cut(s) 975, 3503
BsmI GAATGC 1 cut(s) 830
Bso31I GGTCTC 4 cut(s) 179, 1859, 3830, 4001
BsoBI CYCGRG 1 cut(s) 1206
Bsp119I TTCGAA 2 cut(s) 30, 322
Bsp1286I GDGCHC 8 cut(s) 158, 228, 1683, 1839, 2029, 2164, 3526, 4040
Bsp13I TCCGGA 2 cut(s) 198, 1860
Bsp1407I TGTACA 1 cut(s) 1595
Bsp19I CCATGG 2 cut(s) 1976, 3926
BspCNI CTCAG 2 cut(s) 1132, 1324
BspEI TCCGGA 2 cut(s) 198, 1860
BspFNI CGCG 1 cut(s) 222
BspHI TCATGA 1 cut(s) 834
BspLI GGNNCC 2 cut(s) 48, 535
BspMAI CTGCAG 1 cut(s) 2284
BspMI ACCTGC 2 cut(s) 1571, 3374
BspOI GCTAGC 2 cut(s) 1968, 3234
BspPI GGATC 5 cut(s) 385, 809, 1918, 2069, 3667
BspQI GCTCTTC 1 cut(s) 2718
BspT104I TTCGAA 2 cut(s) 30, 322
BspTNI GGTCTC 4 cut(s) 179, 1859, 3830, 4001
BsrDI GCAATG 4 cut(s) 690, 1893, 2177, 3681
BsrFI RCCGGY 3 cut(s) 3196, 3893, 4024
BsrGI TGTACA 1 cut(s) 1595
BssAI RCCGGY 3 cut(s) 3196, 3893, 4024
BssECI CCNNGG 7 cut(s) 1976, 3088, 3657, 3658, 3926, 4066, 4078
BssNAI GTATAC 2 cut(s) 2438, 3703
BssNI GRCGYC 2 cut(s) 195, 2547
BssSI CACGAG 2 cut(s) 411, 1512
BssT1I CCWWGG 4 cut(s) 1976, 3088, 3926, 4066
Bst1107I GTATAC 2 cut(s) 2438, 3703
Bst2BI CACGAG 2 cut(s) 411, 1512
Bst2UI CCWGG 8 cut(s) 1387, 1568, 2490, 2820, 3175, 3659, 3845, 4080
Bst6I CTCTTC 5 cut(s) 1862, 2682, 2718, 3264, 3865
BstACI GRCGYC 2 cut(s) 195, 2547
BstAPI GCANNNNNTGC 1 cut(s) 1877
BstAUI TGTACA 1 cut(s) 1595
BstBAI YACGTR 2 cut(s) 1189, 3333
BstBI TTCGAA 2 cut(s) 30, 322
BstDEI CTNAG 6 cut(s) 718, 1140, 1311, 2228, 2795, 3971
BstDSI CCRYGG 2 cut(s) 1976, 3926
BstEII GGTNACC 2 cut(s) 2603, 3187
BstENI CCTNNNNNAGG 1 cut(s) 2723
BstF5I GGATG 9 cut(s) 522, 1030, 1411, 1765, 2911, 3183, 3478, 3903, 3918
BstFNI CGCG 1 cut(s) 222
BstHHI GCGC 1 cut(s) 284
BstMCI CGRYCG 2 cut(s) 71, 211
BstMWI GCNNNNNNNGC 8 cut(s) 139, 219, 1877, 2168, 2636, 2829, 2936, 3650
BstNI CCWGG 8 cut(s) 1387, 1568, 2490, 2820, 3175, 3659, 3845, 4080
BstNSI RCATGY 4 cut(s) 943, 1506, 2413, 2804
BstPI GGTNACC 2 cut(s) 2603, 3187
BstSCI CCNGG 9 cut(s) 508, 1385, 1566, 2488, 2818, 3173, 3657, 3843, 4078
BstSFI CTRYAG 3 cut(s) 981, 1107, 2280
BstSLI GKGCMC 3 cut(s) 1683, 1839, 4040
BstUI CGCG 1 cut(s) 222
BstV2I GAAGAC 2 cut(s) 1436, 3228
BstX2I RGATCY 2 cut(s) 814, 3672
BstXI CCANNNNNNTGG 3 cut(s) 2542, 3174, 3963
BstYI RGATCY 2 cut(s) 814, 3672
BstZ17I GTATAC 2 cut(s) 2438, 3703
BstZI CGGCCG 1 cut(s) 68
BsuI GTATCC 1 cut(s) 2567
BtgI CCRYGG 2 cut(s) 1976, 3926
BtsCI GGATG 9 cut(s) 522, 1030, 1411, 1765, 2911, 3183, 3478, 3903, 3918
BtsIMutI CAGTG 2 cut(s) 2250, 4038
BveI ACCTGC 2 cut(s) 1571, 3374
CaiI CAGNNNCTG 1 cut(s) 4103
CciI TCATGA 1 cut(s) 834
CfoI GCGC 1 cut(s) 284
Cfr10I RCCGGY 3 cut(s) 3196, 3893, 4024
CpoI CGGWCCG 1 cut(s) 4022
CsiI ACCWGGT 1 cut(s) 2488
CspI CGGWCCG 1 cut(s) 4022
DdeI CTNAG 6 cut(s) 718, 1140, 1311, 2228, 2795, 3971
DraI TTTAAA 2 cut(s) 454, 474
DraIII CACNNNGTG 1 cut(s) 2492
EaeI YGGCCR 1 cut(s) 68
EagI CGGCCG 1 cut(s) 68
Eam1104I CTCTTC 5 cut(s) 1862, 2682, 2718, 3264, 3865
EarI CTCTTC 5 cut(s) 1862, 2682, 2718, 3264, 3865
EciI GGCGGA 1 cut(s) 107
Ecl136II GAGCTC 2 cut(s) 226, 3524
EclXI CGGCCG 1 cut(s) 68
Eco130I CCWWGG 4 cut(s) 1976, 3088, 3926, 4066
Eco24I GRGCYC 2 cut(s) 228, 3526
Eco31I GGTCTC 4 cut(s) 179, 1859, 3830, 4001
Eco32I GATATC 5 cut(s) 1722, 2001, 2070, 2590, 2598
Eco47I GGWCC 5 cut(s) 533, 1001, 1560, 3363, 4022
Eco52I CGGCCG 1 cut(s) 68
Eco53kI GAGCTC 2 cut(s) 226, 3524
Eco57I CTGAAG 4 cut(s) 1112, 1200, 3552, 4117
Eco88I CYCGRG 1 cut(s) 1206
Eco91I GGTNACC 2 cut(s) 2603, 3187
EcoICRI GAGCTC 2 cut(s) 226, 3524
EcoNI CCTNNNNNAGG 1 cut(s) 2723
EcoO109I RGGNCCY 4 cut(s) 533, 1202, 1560, 3661
EcoO65I GGTNACC 2 cut(s) 2603, 3187
EcoRII CCWGG 8 cut(s) 1385, 1566, 2488, 2818, 3173, 3657, 3843, 4078
EcoRV GATATC 5 cut(s) 1722, 2001, 2070, 2590, 2598
EcoT14I CCWWGG 4 cut(s) 1976, 3088, 3926, 4066
EcoT22I ATGCAT 2 cut(s) 832, 2179
EcoT38I GRGCYC 2 cut(s) 228, 3526
ErhI CCWWGG 4 cut(s) 1976, 3088, 3926, 4066
Esp3I CGTCTC 2 cut(s) 278, 457
FalI AAGNNNNNCTT 8 cut(s) 282, 314, 505, 537, 2788, 2820, 4050, 4082
FaqI GGGAC 2 cut(s) 975, 3503
FauI CCCGC 2 cut(s) 3149, 3930
FauNDI CATATG 2 cut(s) 1426, 2014
FbaI TGATCA 2 cut(s) 1324, 1527
FblI GTMKAC 2 cut(s) 2437, 3702
FokI GGATG 9 cut(s) 529, 1037, 1418, 1752, 2918, 3190, 3485, 3890, 3905
FriOI GRGCYC 2 cut(s) 228, 3526
FspBI CTAG 9 cut(s) 438, 732, 1437, 1608, 1965, 2517, 2777, 3089, 3231
GlaI GCGC 1 cut(s) 283
GsuI CTGGAG 4 cut(s) 574, 2346, 3203, 3282
HapII CCGG 6 cut(s) 199, 510, 1861, 3197, 3894, 4025
HhaI GCGC 1 cut(s) 284
Hin1I GRCGYC 2 cut(s) 195, 2547
Hin6I GCGC 1 cut(s) 282
HinP1I GCGC 1 cut(s) 282
HincII GTYRAC 2 cut(s) 2395, 2407
HindII GTYRAC 2 cut(s) 2395, 2407
HindIII AAGCTT 1 cut(s) 632
HpaI GTTAAC 1 cut(s) 2395
HpaII CCGG 6 cut(s) 199, 510, 1861, 3197, 3894, 4025
Hpy166II GTNNAC 8 cut(s) 1681, 1837, 2395, 2407, 2438, 3206, 3404, 3703
Hpy8I GTNNAC 8 cut(s) 1681, 1837, 2395, 2407, 2438, 3206, 3404, 3703
Hpy99I CGWCG 4 cut(s) 76, 142, 197, 233
HpyF10VI GCNNNNNNNGC 8 cut(s) 139, 219, 1877, 2168, 2636, 2829, 2936, 3650
HpyF3I CTNAG 6 cut(s) 718, 1140, 1311, 2228, 2795, 3971
Hsp92I GRCGYC 2 cut(s) 195, 2547
HspAI GCGC 1 cut(s) 282
Kpn2I TCCGGA 2 cut(s) 198, 1860
Ksp22I TGATCA 2 cut(s) 1324, 1527
KspAI GTTAAC 1 cut(s) 2395
LguI GCTCTTC 1 cut(s) 2718
LmnI GCTCC 6 cut(s) 18, 106, 1426, 1489, 2522, 3521
LweI GCATC 6 cut(s) 17, 817, 851, 1880, 2917, 3168
MabI ACCWGGT 1 cut(s) 2488
MaeI CTAG 9 cut(s) 438, 732, 1437, 1608, 1965, 2517, 2777, 3089, 3231
MaeIII GTNAC 7 cut(s) 976, 1288, 1588, 2197, 2427, 2603, 3187
MfeI CAATTG 6 cut(s) 924, 1328, 2091, 2188, 3579, 3588
MflI RGATCY 2 cut(s) 814, 3672
MhlI GDGCHC 8 cut(s) 158, 228, 1683, 1839, 2029, 2164, 3526, 4040
MmeI TCCRAC 7 cut(s) 162, 198, 910, 979, 1200, 2445, 4000
Mph1103I ATGCAT 2 cut(s) 832, 2179
MroI TCCGGA 2 cut(s) 198, 1860
MroXI GAANNNNTTC 1 cut(s) 3631
MslI CAYNNNNRTG 4 cut(s) 1777, 2540, 2948, 3686
MspA1I CMGCKG 6 cut(s) 495, 1916, 3302, 3623, 3653, 3851
MspI CCGG 6 cut(s) 199, 510, 1861, 3197, 3894, 4025
MspR9I CCNGG 9 cut(s) 510, 1387, 1568, 2490, 2820, 3175, 3659, 3845, 4080
MunI CAATTG 6 cut(s) 924, 1328, 2091, 2188, 3579, 3588
Mva1269I GAATGC 1 cut(s) 830
MvaI CCWGG 8 cut(s) 1387, 1568, 2490, 2820, 3175, 3659, 3845, 4080
MvnI CGCG 1 cut(s) 222
MwoI GCNNNNNNNGC 8 cut(s) 139, 219, 1877, 2168, 2636, 2829, 2936, 3650
NciI CCSGG 1 cut(s) 510
NcoI CCATGG 2 cut(s) 1976, 3926
NdeI CATATG 2 cut(s) 1426, 2014
NheI GCTAGC 2 cut(s) 1964, 3230
NlaIV GGNNCC 2 cut(s) 48, 535
NmeAIII GCCGAG 3 cut(s) 46, 106, 1705
NmuCI GTSAC 3 cut(s) 1588, 2197, 2427
NsiI ATGCAT 2 cut(s) 832, 2179
NspI RCATGY 4 cut(s) 943, 1506, 2413, 2804
NspV TTCGAA 2 cut(s) 30, 322
PaeI GCATGC 2 cut(s) 1506, 2804
PaeR7I CTCGAG 1 cut(s) 1206
PagI TCATGA 1 cut(s) 834
PasI CCCWGGG 1 cut(s) 3658
PciI ACATGT 1 cut(s) 939
PciSI GCTCTTC 1 cut(s) 2718
PcsI WCGNNNNNNNCGW 2 cut(s) 252, 363
PctI GAATGC 1 cut(s) 830
PdmI GAANNNNTTC 1 cut(s) 3631
PfeI GAWTC 6 cut(s) 39, 1947, 2444, 3138, 3148, 3883
Pfl23II CGTACG 1 cut(s) 1338
PflFI GACNNNGTC 1 cut(s) 2305
PflMI CCANNNNNTGG 1 cut(s) 545
Ple19I CGATCG 1 cut(s) 211
Ppu21I YACGTR 2 cut(s) 1189, 3333
PpuMI RGGWCCY 2 cut(s) 533, 1560
PscI ACATGT 1 cut(s) 939
Psp124BI GAGCTC 2 cut(s) 228, 3526
Psp5II RGGWCCY 2 cut(s) 533, 1560
Psp6I CCWGG 8 cut(s) 1385, 1566, 2488, 2818, 3173, 3657, 3843, 4078
PspEI GGTNACC 2 cut(s) 2603, 3187
PspGI CCWGG 8 cut(s) 1385, 1566, 2488, 2818, 3173, 3657, 3843, 4078
PspLI CGTACG 1 cut(s) 1338
PspN4I GGNNCC 2 cut(s) 48, 535
PspPPI RGGWCCY 2 cut(s) 533, 1560
PspXI VCTCGAGB 1 cut(s) 1206
PsrI GAACNNNNNNTAC 2 cut(s) 2106, 2138
PstI CTGCAG 1 cut(s) 2284
PstNI CAGNNNCTG 1 cut(s) 4103
PsuI RGATCY 2 cut(s) 814, 3672
PsyI GACNNNGTC 1 cut(s) 2305
PvuI CGATCG 1 cut(s) 211
PvuII CAGCTG 4 cut(s) 1916, 3302, 3623, 3851
RseI CAYNNNNRTG 4 cut(s) 1777, 2540, 2948, 3686
Rsr2I CGGWCCG 1 cut(s) 4022
RsrII CGGWCCG 1 cut(s) 4022
SacI GAGCTC 2 cut(s) 228, 3526
SapI GCTCTTC 1 cut(s) 2718
ScaI AGTACT 3 cut(s) 26, 1113, 1316
ScrFI CCNGG 9 cut(s) 510, 1387, 1568, 2490, 2820, 3175, 3659, 3845, 4080
SduI GDGCHC 8 cut(s) 158, 228, 1683, 1839, 2029, 2164, 3526, 4040
SexAI ACCWGGT 1 cut(s) 2488
SfaNI GCATC 6 cut(s) 17, 817, 851, 1880, 2917, 3168
SfcI CTRYAG 3 cut(s) 981, 1107, 2280
Sfr274I CTCGAG 1 cut(s) 1206
SfuI TTCGAA 2 cut(s) 30, 322
SinI GGWCC 5 cut(s) 533, 1001, 1560, 3363, 4022
SlaI CTCGAG 1 cut(s) 1206
SmiMI CAYNNNNRTG 4 cut(s) 1777, 2540, 2948, 3686
SmlI CTYRAG 3 cut(s) 167, 1206, 2750
SmoI CTYRAG 3 cut(s) 167, 1206, 2750
SpeI ACTAGT 2 cut(s) 437, 2776
SphI GCATGC 2 cut(s) 1506, 2804
SspMI CTAG 9 cut(s) 438, 732, 1437, 1608, 1965, 2517, 2777, 3089, 3231
SstI GAGCTC 2 cut(s) 228, 3526
StyD4I CCNGG 9 cut(s) 508, 1385, 1566, 2488, 2818, 3173, 3657, 3843, 4078
StyI CCWWGG 4 cut(s) 1976, 3088, 3926, 4066
TaqII GACCGA 1 cut(s) 2318
TatI WGTACW 5 cut(s) 24, 1111, 1314, 1595, 3255
TauI GCSGC 5 cut(s) 8, 122, 133, 1454, 3656
TfiI GAWTC 6 cut(s) 39, 1947, 2444, 3138, 3148, 3883
TscAI CASTG 2 cut(s) 2250, 4045
TseFI GTSAC 3 cut(s) 1588, 2197, 2427
Tsp45I GTSAC 3 cut(s) 1588, 2197, 2427
TspGWI ACGGA 1 cut(s) 1799
TspRI CASTG 2 cut(s) 2250, 4045
Tth111I GACNNNGTC 1 cut(s) 2305
Van91I CCANNNNNTGG 1 cut(s) 545
VneI GTGCAC 2 cut(s) 1679, 1835
VpaK11BI GGWCC 5 cut(s) 533, 1001, 1560, 3363, 4022
XagI CCTNNNNNAGG 1 cut(s) 2723
XapI RAATTY 4 cut(s) 1084, 2042, 3752, 4157
XbaI TCTAGA 1 cut(s) 1607
XceI RCATGY 4 cut(s) 943, 1506, 2413, 2804
XcmI CCANNNNNNNNNTGG 2 cut(s) 1983, 3933
XhoI CTCGAG 1 cut(s) 1206
XmaJI CCTAGG 1 cut(s) 3088
XmiI GTMKAC 2 cut(s) 2437, 3702
XmnI GAANNNNTTC 1 cut(s) 3631
XspI CTAG 9 cut(s) 438, 732, 1437, 1608, 1965, 2517, 2777, 3089, 3231
ZraI GACGTC 2 cut(s) 196, 2548
ZrmI AGTACT 3 cut(s) 26, 1113, 1316
Zsp2I ATGCAT 2 cut(s) 832, 2179
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.