Rh5DG485400
ERF Family

Belongs to the ABC transporter superfamily. ABCG family. PDR (TC 3.A.1.205) subfamily

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr5D
OG0007618
Physical Location & Seq
Forward (+)
76311304 .. 76319361
8058 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh5DG485400.1

Sequence Viewer

Length: 3750 bp
ATGACTTTGCTTTTAGGACCCCCAAGTTCTGGCAAATCCACTTTGCTCACGACTCTTGCAGGAAAACTGGACTCCAGCTTAAAGAAAAGTGGTAGCATTACCTACAATGGGCATAAGCTTGATGAGTTCTGCGTTCAAAGAACTGCTGCCTACATAAGCCAAACAGATAATCATATTGCAGAACTCACCGTGAGAGAAACCTTAGACTTTGCAGCTAGATGTCAAGGTGCAAGCGAAGGTTTTGCAGCGTACATGAAAAATCTGGTCAAGTTAGAGAAGGAAAAAGACATAAGGCCAGATCCAGTAATTGATGCATTCATGAAGGCATCATCTGTTGGTGGTAAAAAGCACAGTATTACAACAGATTATGTTCTCAAAGTGCTTGGTCTTGATGTTTGTTCAGAGACAATTGTTGGAAACGACATGTTAAGAGGGGTTTCTGGGGGACAAAGGAAAAGGGTTACTACAGGTGAAATGGCAGTTGGACCAAGAAAAACCCTGTTTATGGATGAAATATCTACTGGATTGGATAGTTCTACTACATACCAAATTGTAAAATGTATTGGGAATTTTGTTCATCACATGGAAGCTACAGTACTAATGGCTCTGCTTCAGCCTGCACCTGAGACATTTGAATTGTTTGATGATTTAGTGCTACTATCTGAAGGCTACGTGGTTTATCAAGGTCCTCGAGCAGAAGTGTTGGAGTTCTTCGAGTCATTAGGCTTCAAACTACCACCACGAAAGGGTGTTGCAGATTTTCTTCAAGAGGTAACGTCTAAAAAAGATCAAGCTCAGTACTGGGCTGATCGTTCGAAACCATACGCATACATTTCTGTTTCAGAAATTGCAGAAGCCTTTAAGAAGTCCAGGTTTGGAAGGTCTGTGGATGACACTCTCTCTGCTCCATATGATAAATCTAGCAGTCTTCCTGCGGCTTTGCCAAAAACTAAATATGCAACCTCAAAATGGGAGCTTTGTAAAGCATGCTTTTCACGAGAACTACTGTTGATCAGCAGGCATAGGTTTCTTTACACATTTAGGACCTGCCAGGTTGCATTTGTTGGGGCTGTGACATGTACAATGTTTTCTAGATTGAAATTACACCCGACAGATGAAATAAATGGCAACCTTTATCTTTCTTGCTTGTTCTTTGGGTTGGTGCACATGATGTTTAATGGGTTTTCCGAGCTACCTCTTATGATATCTCGGCTTCCAGTCTTTTACAAGCAAAGAGACAATCACTTTCATCCTGCATGGGCATGGTCTGTTGTTAGTTTGCTTCTGCGCATACCTTACTCTATTATCGAAGCTGTTGTATGGTCTTGTGTCGTATACTACTCGGTTGGATTTGCCCCTGGTGCTGGGAGATTTTTCCGTTTCATGTTTTTGCTGTTCTCTGTGCACCAAATGGCTTTGGGTCTCTTCCGGACAATGGCATCTCTTGCAAGAGATATGGTCATTGCCAATACATTTGGATCAGCTGCACTAATTATTATATTTTCTTTTGGTGGATTCATCATTCCCAAAGAGAGCATTAAGCCATGGTGGGAATGGGCCTTTTGGATATCACCACTATCATATGGACAACGTGCTCTTTCTGTCAATGAATTTACGGCTGTAAGGTGGATCAAGATATCTCCTCTAACTAACAGAACAATTGGGCAAAGTGTTCTCCAAGAACACAACTTACCAAATGGTGATTACTGGTATTGGATTGGAGTTGGTGCTCTGTTAGCTTATGCATTGCTTTTCAATTGCATAGTGACTCTGACCTTGATGTACCTAAATCCACTAAGGAAAAATCAGACAGTGCTTCCACTTGATGACACACAAGAGAGTCCTGCTGCAGATGGGGGTAAATATAAGACCGAGTCTATCCCAAAATCAACTGGAGATAACGGCCCAAGAAAGGGAATGATCTTACCATTTCAGCCATTAACAATGACTTTCCACAATGTTAACTACTCTGTTGACATGCCAAAGGAAATGAAGTCACAAGGTATACCAGAATCGAAGTTGAAACTCTTGTCGGAGGTGAGTGGAGTATTCTCACCAGGTGTTCTTACAGCTTTAGTTGGGTCTAGTGGAGCTGGAAAGACCACTTTGATGGACGTCCTTGCTGGTAGGAAAACTGGTGGATACATAGAAGGAGATATCAAGATATCTGGTTACCCGAAAGAGCAACGTACTTTTGCAAGAATAGCAGGATATGTTGAGCAAAATGACATACATTCTCCTCAACTTACAGTTGAAGAGTCTCTATGGTTTTCTTCTGCTCTTCGCCTTCCGAAGGAAGTCAGCACAGAGAAAAGACTTGAGTTTGTCGAAGAAGTAATGAAACTAGTAGAGCTTGATACTCTAAGGCATGCTTTGGTTGGTTTGCCAGGCAGTTCAGGCTTATCAACAGAGCAAAGAAAACGCTTAACAATTTCTGTTGAGCTTGTTGCCAACCCTTCAATTATCTTTATGGATGAACCCACATCTGGACTTGATGCAAGGGCAGCAGCCATTGTGATGAGGACTGTTCGTAATACAGTTGATACAGGAAGAACGGTGGTCTGCACTATACATCAACCAAGTATTGACATATTTGAGGCATTTGATGAGCTTCTTCTTATGAAACGAGGGGGGCAAGTTATTTATGGAGGAAACCTAGGCCAGCACTCCCAGACAATGATAAACTACTTTCAGGAGATCAATGGAATCACTCCAATTCCAAGCGGGTACAATCCAGCAACCTGGATGCTGGAGGTAACCACACCGGCTTGTGAACAGAGAATTGGGGAAGACTTCGCTAGCATATACAGAAAATCAGAGCAGTACAGGGAGGTGGAAGAGTCCATCAAGCAGTTTAGCACTCCTCCAGCTGACTCAAAACCATTAGAGTTTGCTTCCACGTATTCACAAAACACATTGGCTCAATTTTGGACCTGCTTATGGAAACAAAATCTTGTGTATTGGAGAAGTCCACAATATAATGCCATGAGGTTAATCTTTACTACGCTTGCTGCATTGATATTCGGTTCAGCATTTTGGGATGTTGGTACAAAAAGGGACTCAATTCAATCATTGATGATGGTTATGGGAGCTCTTTATTCTGCTTGCCTGTTTCTTGGGGTCAATAACGCTTCTTCAGTTCAGCCAATTGTTTCAATTGAGAGGACAGTATTTTATCGAGAGAAAGCAGCTGGAATGTATTCTCCTTTCGCTTATGCAGCGGCCCAGGGCCTTATAGAGATCCCATACATTGCTGTACAGACAATAGTATACGGGGTGATCACATACTTCATGGTCAATTTTGAAAGGACAATCACAAAATTCTTGCTCTATATTCTGTTCATGTTCCTCACATTCACCTACTTCACCCTTTATGGCATGGCGGCTGTTGGTCTCACTCCCTCTCAACACCTGGCAGCTGTCATCTCTTCTGCATTTTACTCATTGTGGAATCTCCTCTCCGGTTTCCTCATCCCCAAACCAAACATCCCGCCATGGTGGATATGGTTTTACTACATCACCCCAGTTGCATGGACACTTAGAGGTATTATCACTTCCCAACTTGGTGATGTGGAGACCATTCTTGTCGGACCTGCGTTTAAGGGCACTGTGAAAGAATACTTGGAAGTCAGCCTTGGCTATGGCCCTGGAATGATTGGAGTTTCAGTAGCTGTGCTACTGGGTTTCAACGTCCTTTTCTTCAGTATCTTTGCCTTCTCCATCAAATTTCTTAACTTCCAGAGAAGATAG

Protein Analysis

1249

Amino Acids

139.6

Weight (kDa)

8.57

Isoelectric Point (pI)

34.83

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
ABC_tran PF00005 2 - 171 4.2e-13 ABC transporter
ABC2_membrane_7 PF19055 205 - 255 4.1e-06 ABC-2 type transporter
ABC2_membrane PF01061 326 - 538 3.1e-49 ABC-2 type transporter
PDR_assoc PF08370 543 - 605 8.4e-23 Plant PDR ABC transporter associated
ABC_tran PF00005 678 - 829 5.4e-20 ABC transporter
ABC2_membrane PF01061 975 - 1187 2.1e-58 ABC-2 type transporter
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 2118
Acc16I TGCGCA 1 cut(s) 1289
Acc36I ACCTGC 3 cut(s) 1055, 2942, 3601
AccB7I CCANNNNNTGG 1 cut(s) 29
AccI GTMKAC 3 cut(s) 1335, 2005, 3270
AccIII TCCGGA 1 cut(s) 1428
AciI CCGC 5 cut(s) 935, 2724, 3221, 3383, 3491
AclWI GGATC 4 cut(s) 293, 1486, 1637, 3235
AcsI RAATTY 4 cut(s) 568, 1610, 3320, 3725
AcuI CTGAAG 4 cut(s) 596, 684, 3120, 3685
AcyI GRCGYC 1 cut(s) 2115
AdeI CACNNNGTG 1 cut(s) 2060
AflIII ACRYGT 2 cut(s) 423, 1076
AhlI ACTAGT 1 cut(s) 2344
AjnI CCWGG 9 cut(s) 869, 1050, 1357, 2056, 2386, 2741, 3225, 3411, 3646
AjuI GAANNNNNNNTTGG 8 cut(s) 465, 497, 2766, 2798, 3605, 3618, 3637, 3650
Alw21I GWGCWC 5 cut(s) 1167, 1407, 1597, 1732, 3094
Alw26I GTCTC 7 cut(s) 398, 620, 1230, 1427, 2265, 3398, 3569
Alw44I GTGCAC 2 cut(s) 1163, 1403
AlwI GGATC 4 cut(s) 293, 1486, 1637, 3235
AlwNI CAGNNNCTG 1 cut(s) 3671
Ama87I CYCGRG 1 cut(s) 690
Aor13HI TCCGGA 1 cut(s) 1428
AoxI GGCC 7 cut(s) 293, 1557, 1903, 2659, 3222, 3229, 3643
ApaLI GTGCAC 2 cut(s) 1163, 1403
ApoI RAATTY 4 cut(s) 568, 1610, 3320, 3725
Asp700I GAANNNNTTC 1 cut(s) 3199
AspA2I CCTAGG 1 cut(s) 2656
AspLEI GCGC 1 cut(s) 1290
AsuII TTCGAA 1 cut(s) 815
AsuNHI GCTAGC 1 cut(s) 2798
AvaI CYCGRG 1 cut(s) 690
AvaII GGWCC 6 cut(s) 17, 485, 686, 1044, 2931, 3590
AvrII CCTAGG 1 cut(s) 2656
BaeGI GKGCMC 3 cut(s) 1167, 1407, 3608
BanII GRGCYC 1 cut(s) 3094
BarI GAAGNNNNNNTAC 4 cut(s) 579, 611, 3538, 3570
BauI CACGAG 1 cut(s) 996
BbsI GAAGAC 2 cut(s) 920, 2796
Bbv12I GWGCWC 5 cut(s) 1167, 1407, 1597, 1732, 3094
BccI CCATC 5 cut(s) 1847, 2104, 2852, 3073, 3728
BceAI ACGGC 2 cut(s) 1632, 1918
BcgI CGANNNNNNTGC 2 cut(s) 224, 258
BciT130I CCWGG 9 cut(s) 871, 1052, 1359, 2058, 2388, 2743, 3227, 3413, 3648
BciVI GTATCC 1 cut(s) 2135
BclI TGATCA 2 cut(s) 1011, 3279
BcoDI GTCTC 7 cut(s) 398, 620, 1230, 1427, 2265, 3398, 3569
BcuI ACTAGT 1 cut(s) 2344
BfaI CTAG 7 cut(s) 216, 921, 1092, 2085, 2345, 2657, 2799
BfmI CTRYAG 3 cut(s) 465, 591, 1848
BfuAI ACCTGC 3 cut(s) 1055, 2942, 3601
BfuI GTATCC 1 cut(s) 2135
BlnI CCTAGG 1 cut(s) 2656
BmcAI AGTACT 2 cut(s) 597, 800
Bme1390I CCNGG 9 cut(s) 871, 1052, 1359, 2058, 2388, 2743, 3227, 3413, 3648
Bme18I GGWCC 6 cut(s) 17, 485, 686, 1044, 2931, 3590
BmeT110I CYCGRG 1 cut(s) 690
BmiI GGNNCC 1 cut(s) 19
BmrFI CCNGG 9 cut(s) 871, 1052, 1359, 2058, 2388, 2743, 3227, 3413, 3648
BmrI ACTGGG 3 cut(s) 811, 3518, 3689
BmsI GCATC 5 cut(s) 301, 335, 1448, 2485, 2736
BmtI GCTAGC 1 cut(s) 2802
BmuI ACTGGG 3 cut(s) 811, 3518, 3689
BpiI GAAGAC 2 cut(s) 920, 2796
BpmI CTGGAG 4 cut(s) 58, 1914, 2771, 2850
Bpu14I TTCGAA 1 cut(s) 815
BpuEI CTTGAG 1 cut(s) 2339
BsaAI YACGTR 2 cut(s) 673, 2901
BsaHI GRCGYC 1 cut(s) 2115
BsaI GGTCTC 3 cut(s) 1427, 3398, 3569
BsaJI CCNNGG 8 cut(s) 1357, 1544, 2656, 3225, 3226, 3494, 3634, 3646
BsaWI WCCGGW 2 cut(s) 1428, 3461
BsaXI ACNNNNNCTCC 2 cut(s) 3651, 3681
Bse118I RCCGGY 1 cut(s) 2764
Bse3DI GCAATG 3 cut(s) 1461, 1745, 3249
BseAI TCCGGA 1 cut(s) 1428
BseBI CCWGG 9 cut(s) 871, 1052, 1359, 2058, 2388, 2743, 3227, 3413, 3648
BseDI CCNNGG 8 cut(s) 1357, 1544, 2656, 3225, 3226, 3494, 3634, 3646
BseGI GGATG 8 cut(s) 514, 895, 1249, 2479, 2751, 3046, 3471, 3486
BseMI GCAATG 3 cut(s) 1461, 1745, 3249
BseMII CTCAG 2 cut(s) 615, 809
BseRI GAGGAG 4 cut(s) 1632, 2229, 2853, 3446
BseSI GKGCMC 3 cut(s) 1167, 1407, 3608
BseYI CCCAGC 1 cut(s) 1364
BsgI GTGCAG 3 cut(s) 603, 1470, 2548
BshFI GGCC 7 cut(s) 295, 1559, 1905, 2661, 3224, 3231, 3645
BsiHKAI GWGCWC 5 cut(s) 1167, 1407, 1597, 1732, 3094
BsiHKCI CYCGRG 1 cut(s) 690
BsiSI CCGG 3 cut(s) 1429, 2765, 3462
BslFI GGGAC 2 cut(s) 459, 3071
BsmAI GTCTC 7 cut(s) 398, 620, 1230, 1427, 2265, 3398, 3569
BsmFI GGGAC 2 cut(s) 459, 3071
BsmI GAATGC 1 cut(s) 314
BsnI GGCC 7 cut(s) 295, 1559, 1905, 2661, 3224, 3231, 3645
Bso31I GGTCTC 3 cut(s) 1427, 3398, 3569
BsoBI CYCGRG 1 cut(s) 690
Bsp119I TTCGAA 1 cut(s) 815
Bsp1286I GDGCHC 6 cut(s) 1167, 1407, 1597, 1732, 3094, 3608
Bsp13I TCCGGA 1 cut(s) 1428
Bsp1407I TGTACA 2 cut(s) 1079, 3256
Bsp19I CCATGG 2 cut(s) 1544, 3494
BspACI CCGC 5 cut(s) 935, 2724, 3221, 3383, 3491
BspANI GGCC 7 cut(s) 295, 1559, 1905, 2661, 3224, 3231, 3645
BspCNI CTCAG 2 cut(s) 616, 808
BspEI TCCGGA 1 cut(s) 1428
BspHI TCATGA 1 cut(s) 318
BspLI GGNNCC 1 cut(s) 19
BspMAI CTGCAG 1 cut(s) 1852
BspMI ACCTGC 3 cut(s) 1055, 2942, 3601
BspOI GCTAGC 1 cut(s) 2802
BspPI GGATC 4 cut(s) 293, 1486, 1637, 3235
BspQI GCTCTTC 1 cut(s) 2286
BspT104I TTCGAA 1 cut(s) 815
BspTNI GGTCTC 3 cut(s) 1427, 3398, 3569
BsrDI GCAATG 3 cut(s) 1461, 1745, 3249
BsrFI RCCGGY 1 cut(s) 2764
BsrGI TGTACA 2 cut(s) 1079, 3256
BssAI RCCGGY 1 cut(s) 2764
BssECI CCNNGG 8 cut(s) 1357, 1544, 2656, 3225, 3226, 3494, 3634, 3646
BssNAI GTATAC 3 cut(s) 1336, 2006, 3271
BssNI GRCGYC 1 cut(s) 2115
BssSI CACGAG 1 cut(s) 996
BssT1I CCWWGG 4 cut(s) 1544, 2656, 3494, 3634
Bst1107I GTATAC 3 cut(s) 1336, 2006, 3271
Bst2BI CACGAG 1 cut(s) 996
Bst2UI CCWGG 9 cut(s) 871, 1052, 1359, 2058, 2388, 2743, 3227, 3413, 3648
Bst6I CTCTTC 5 cut(s) 1430, 2250, 2286, 2832, 3433
BstACI GRCGYC 1 cut(s) 2115
BstAPI GCANNNNNTGC 1 cut(s) 1445
BstAUI TGTACA 2 cut(s) 1079, 3256
BstBAI YACGTR 2 cut(s) 673, 2901
BstBI TTCGAA 1 cut(s) 815
BstC8I GCNNGC 9 cut(s) 232, 618, 988, 1019, 2370, 2663, 2800, 3009, 3106
BstDEI CTNAG 6 cut(s) 202, 624, 795, 1796, 2363, 3539
BstDSI CCRYGG 2 cut(s) 1544, 3494
BstEII GGTNACC 2 cut(s) 2171, 2755
BstENI CCTNNNNNAGG 1 cut(s) 2291
BstF5I GGATG 8 cut(s) 514, 895, 1249, 2479, 2751, 3046, 3471, 3486
BstHHI GCGC 1 cut(s) 1290
BstMAI GTCTC 7 cut(s) 398, 620, 1230, 1427, 2265, 3398, 3569
BstMWI GCNNNNNNNGC 7 cut(s) 1361, 1445, 1736, 2204, 2397, 2504, 3218
BstNI CCWGG 9 cut(s) 871, 1052, 1359, 2058, 2388, 2743, 3227, 3413, 3648
BstNSI RCATGY 5 cut(s) 427, 990, 1080, 1981, 2372
BstPI GGTNACC 2 cut(s) 2171, 2755
BstSCI CCNGG 9 cut(s) 869, 1050, 1357, 2056, 2386, 2741, 3225, 3411, 3646
BstSFI CTRYAG 3 cut(s) 465, 591, 1848
BstSLI GKGCMC 3 cut(s) 1167, 1407, 3608
BstV2I GAAGAC 2 cut(s) 920, 2796
BstX2I RGATCY 2 cut(s) 298, 3240
BstXI CCANNNNNNTGG 3 cut(s) 2110, 2742, 3531
BstYI RGATCY 2 cut(s) 298, 3240
BstZ17I GTATAC 3 cut(s) 1336, 2006, 3271
BsuI GTATCC 1 cut(s) 2135
BsuRI GGCC 7 cut(s) 295, 1559, 1905, 2661, 3224, 3231, 3645
BtgI CCRYGG 2 cut(s) 1544, 3494
BtsCI GGATG 8 cut(s) 514, 895, 1249, 2479, 2751, 3046, 3471, 3486
BtsIMutI CAGTG 2 cut(s) 1818, 3606
BveI ACCTGC 3 cut(s) 1055, 2942, 3601
Cac8I GCNNGC 9 cut(s) 232, 618, 988, 1019, 2370, 2663, 2800, 3009, 3106
CaiI CAGNNNCTG 1 cut(s) 3671
CciI TCATGA 1 cut(s) 318
CfoI GCGC 1 cut(s) 1290
Cfr10I RCCGGY 1 cut(s) 2764
CsiI ACCWGGT 1 cut(s) 2056
DdeI CTNAG 6 cut(s) 202, 624, 795, 1796, 2363, 3539
DraIII CACNNNGTG 1 cut(s) 2060
Eam1104I CTCTTC 5 cut(s) 1430, 2250, 2286, 2832, 3433
EarI CTCTTC 5 cut(s) 1430, 2250, 2286, 2832, 3433
Ecl136II GAGCTC 1 cut(s) 3092
Eco130I CCWWGG 4 cut(s) 1544, 2656, 3494, 3634
Eco24I GRGCYC 1 cut(s) 3094
Eco31I GGTCTC 3 cut(s) 1427, 3398, 3569
Eco32I GATATC 5 cut(s) 1206, 1569, 1638, 2158, 2166
Eco47I GGWCC 6 cut(s) 17, 485, 686, 1044, 2931, 3590
Eco53kI GAGCTC 1 cut(s) 3092
Eco57I CTGAAG 4 cut(s) 596, 684, 3120, 3685
Eco88I CYCGRG 1 cut(s) 690
Eco91I GGTNACC 2 cut(s) 2171, 2755
EcoICRI GAGCTC 1 cut(s) 3092
EcoNI CCTNNNNNAGG 1 cut(s) 2291
EcoO109I RGGNCCY 4 cut(s) 17, 686, 1044, 3229
EcoO65I GGTNACC 2 cut(s) 2171, 2755
EcoRII CCWGG 9 cut(s) 869, 1050, 1357, 2056, 2386, 2741, 3225, 3411, 3646
EcoRV GATATC 5 cut(s) 1206, 1569, 1638, 2158, 2166
EcoT14I CCWWGG 4 cut(s) 1544, 2656, 3494, 3634
EcoT22I ATGCAT 2 cut(s) 316, 1747
EcoT38I GRGCYC 1 cut(s) 3094
ErhI CCWWGG 4 cut(s) 1544, 2656, 3494, 3634
FalI AAGNNNNNCTT 4 cut(s) 2356, 2388, 3618, 3650
FaqI GGGAC 2 cut(s) 459, 3071
FauI CCCGC 2 cut(s) 2717, 3498
FauNDI CATATG 2 cut(s) 910, 1582
FbaI TGATCA 2 cut(s) 1011, 3279
FblI GTMKAC 3 cut(s) 1335, 2005, 3270
FokI GGATG 8 cut(s) 521, 902, 1236, 2486, 2758, 3053, 3458, 3473
FriOI GRGCYC 1 cut(s) 3094
FspBI CTAG 7 cut(s) 216, 921, 1092, 2085, 2345, 2657, 2799
FspI TGCGCA 1 cut(s) 1289
GlaI GCGC 1 cut(s) 1289
GsaI CCCAGC 1 cut(s) 1368
GsuI CTGGAG 4 cut(s) 58, 1914, 2771, 2850
HaeIII GGCC 7 cut(s) 295, 1559, 1905, 2661, 3224, 3231, 3645
HapII CCGG 3 cut(s) 1429, 2765, 3462
HhaI GCGC 1 cut(s) 1290
Hin1I GRCGYC 1 cut(s) 2115
Hin6I GCGC 1 cut(s) 1288
HinP1I GCGC 1 cut(s) 1288
HincII GTYRAC 2 cut(s) 1963, 1975
HindII GTYRAC 2 cut(s) 1963, 1975
HindIII AAGCTT 1 cut(s) 116
HpaI GTTAAC 1 cut(s) 1963
HpaII CCGG 3 cut(s) 1429, 2765, 3462
Hpy166II GTNNAC 9 cut(s) 1165, 1336, 1405, 1963, 1975, 2006, 2774, 2972, 3271
Hpy8I GTNNAC 9 cut(s) 1165, 1336, 1405, 1963, 1975, 2006, 2774, 2972, 3271
HpyCH4IV ACGT 7 cut(s) 672, 776, 1591, 2115, 2188, 2900, 3690
HpyF10VI GCNNNNNNNGC 7 cut(s) 1361, 1445, 1736, 2204, 2397, 2504, 3218
HpyF3I CTNAG 6 cut(s) 202, 624, 795, 1796, 2363, 3539
HpySE526I ACGT 7 cut(s) 672, 776, 1591, 2115, 2188, 2900, 3690
Hsp92I GRCGYC 1 cut(s) 2115
HspAI GCGC 1 cut(s) 1288
Kpn2I TCCGGA 1 cut(s) 1428
Ksp22I TGATCA 2 cut(s) 1011, 3279
KspAI GTTAAC 1 cut(s) 1963
LguI GCTCTTC 1 cut(s) 2286
LmnI GCTCC 4 cut(s) 910, 973, 2090, 3089
LweI GCATC 5 cut(s) 301, 335, 1448, 2485, 2736
MabI ACCWGGT 1 cut(s) 2056
MaeI CTAG 7 cut(s) 216, 921, 1092, 2085, 2345, 2657, 2799
MaeII ACGT 7 cut(s) 672, 776, 1591, 2115, 2188, 2900, 3690
MaeIII GTNAC 7 cut(s) 460, 772, 1072, 1765, 1995, 2171, 2755
MfeI CAATTG 5 cut(s) 408, 1659, 1756, 3147, 3156
MflI RGATCY 2 cut(s) 298, 3240
MhlI GDGCHC 6 cut(s) 1167, 1407, 1597, 1732, 3094, 3608
MmeI TCCRAC 6 cut(s) 394, 463, 684, 1327, 2013, 3568
Mph1103I ATGCAT 2 cut(s) 316, 1747
MroI TCCGGA 1 cut(s) 1428
MroXI GAANNNNTTC 1 cut(s) 3199
MslI CAYNNNNRTG 3 cut(s) 1261, 2108, 2516
MspA1I CMGCKG 5 cut(s) 1484, 2870, 3191, 3221, 3419
MspI CCGG 3 cut(s) 1429, 2765, 3462
MspR9I CCNGG 9 cut(s) 871, 1052, 1359, 2058, 2388, 2743, 3227, 3413, 3648
MunI CAATTG 5 cut(s) 408, 1659, 1756, 3147, 3156
Mva1269I GAATGC 1 cut(s) 314
MvaI CCWGG 9 cut(s) 871, 1052, 1359, 2058, 2388, 2743, 3227, 3413, 3648
MwoI GCNNNNNNNGC 7 cut(s) 1361, 1445, 1736, 2204, 2397, 2504, 3218
NcoI CCATGG 2 cut(s) 1544, 3494
NdeI CATATG 2 cut(s) 910, 1582
NheI GCTAGC 1 cut(s) 2798
NlaIV GGNNCC 1 cut(s) 19
NmeAIII GCCGAG 1 cut(s) 1189
NmuCI GTSAC 3 cut(s) 1072, 1765, 1995
NsbI TGCGCA 1 cut(s) 1289
NsiI ATGCAT 2 cut(s) 316, 1747
NspI RCATGY 5 cut(s) 427, 990, 1080, 1981, 2372
NspV TTCGAA 1 cut(s) 815
PaeI GCATGC 2 cut(s) 990, 2372
PaeR7I CTCGAG 1 cut(s) 690
PagI TCATGA 1 cut(s) 318
PasI CCCWGGG 1 cut(s) 3226
PciI ACATGT 2 cut(s) 423, 1076
PciSI GCTCTTC 1 cut(s) 2286
PctI GAATGC 1 cut(s) 314
PdmI GAANNNNTTC 1 cut(s) 3199
PfeI GAWTC 4 cut(s) 1515, 2012, 2706, 3451
PflFI GACNNNGTC 1 cut(s) 1873
PflMI CCANNNNNTGG 1 cut(s) 29
Ppu21I YACGTR 2 cut(s) 673, 2901
PpuMI RGGWCCY 3 cut(s) 17, 686, 1044
PscI ACATGT 2 cut(s) 423, 1076
Psp124BI GAGCTC 1 cut(s) 3094
Psp5II RGGWCCY 3 cut(s) 17, 686, 1044
Psp6I CCWGG 9 cut(s) 869, 1050, 1357, 2056, 2386, 2741, 3225, 3411, 3646
PspEI GGTNACC 2 cut(s) 2171, 2755
PspFI CCCAGC 1 cut(s) 1364
PspGI CCWGG 9 cut(s) 869, 1050, 1357, 2056, 2386, 2741, 3225, 3411, 3646
PspN4I GGNNCC 1 cut(s) 19
PspPPI RGGWCCY 3 cut(s) 17, 686, 1044
PspXI VCTCGAGB 1 cut(s) 690
PsrI GAACNNNNNNTAC 2 cut(s) 1674, 1706
PstI CTGCAG 1 cut(s) 1852
PstNI CAGNNNCTG 1 cut(s) 3671
PsuI RGATCY 2 cut(s) 298, 3240
PsyI GACNNNGTC 1 cut(s) 1873
PvuII CAGCTG 4 cut(s) 1484, 2870, 3191, 3419
RseI CAYNNNNRTG 3 cut(s) 1261, 2108, 2516
SacI GAGCTC 1 cut(s) 3094
SapI GCTCTTC 1 cut(s) 2286
ScaI AGTACT 2 cut(s) 597, 800
ScrFI CCNGG 9 cut(s) 871, 1052, 1359, 2058, 2388, 2743, 3227, 3413, 3648
SduI GDGCHC 6 cut(s) 1167, 1407, 1597, 1732, 3094, 3608
SexAI ACCWGGT 1 cut(s) 2056
SfaNI GCATC 5 cut(s) 301, 335, 1448, 2485, 2736
SfcI CTRYAG 3 cut(s) 465, 591, 1848
Sfr274I CTCGAG 1 cut(s) 690
SfuI TTCGAA 1 cut(s) 815
SinI GGWCC 6 cut(s) 17, 485, 686, 1044, 2931, 3590
SlaI CTCGAG 1 cut(s) 690
SmiMI CAYNNNNRTG 3 cut(s) 1261, 2108, 2516
SmlI CTYRAG 2 cut(s) 690, 2318
SmoI CTYRAG 2 cut(s) 690, 2318
SpeI ACTAGT 1 cut(s) 2344
SphI GCATGC 2 cut(s) 990, 2372
SsiI CCGC 5 cut(s) 935, 2724, 3221, 3383, 3491
SspMI CTAG 7 cut(s) 216, 921, 1092, 2085, 2345, 2657, 2799
SstI GAGCTC 1 cut(s) 3094
StyD4I CCNGG 9 cut(s) 869, 1050, 1357, 2056, 2386, 2741, 3225, 3411, 3646
StyI CCWWGG 4 cut(s) 1544, 2656, 3494, 3634
TaiI ACGT 7 cut(s) 675, 779, 1594, 2118, 2191, 2903, 3693
TaqI TCGA 7 cut(s) 691, 714, 815, 1308, 2015, 2328, 3178
TaqII GACCGA 1 cut(s) 1886
TatI WGTACW 5 cut(s) 595, 798, 1079, 2823, 3256
TauI GCSGC 3 cut(s) 938, 3224, 3386
TfiI GAWTC 4 cut(s) 1515, 2012, 2706, 3451
TscAI CASTG 2 cut(s) 1818, 3613
TseFI GTSAC 3 cut(s) 1072, 1765, 1995
Tsp45I GTSAC 3 cut(s) 1072, 1765, 1995
TspGWI ACGGA 1 cut(s) 1367
TspRI CASTG 2 cut(s) 1818, 3613
Tth111I GACNNNGTC 1 cut(s) 1873
Van91I CCANNNNNTGG 1 cut(s) 29
VneI GTGCAC 2 cut(s) 1163, 1403
VpaK11BI GGWCC 6 cut(s) 17, 485, 686, 1044, 2931, 3590
XagI CCTNNNNNAGG 1 cut(s) 2291
XapI RAATTY 4 cut(s) 568, 1610, 3320, 3725
XbaI TCTAGA 1 cut(s) 1091
XceI RCATGY 5 cut(s) 427, 990, 1080, 1981, 2372
XcmI CCANNNNNNNNNTGG 2 cut(s) 1551, 3501
XhoI CTCGAG 1 cut(s) 690
XmaJI CCTAGG 1 cut(s) 2656
XmiI GTMKAC 3 cut(s) 1335, 2005, 3270
XmnI GAANNNNTTC 1 cut(s) 3199
XspI CTAG 7 cut(s) 216, 921, 1092, 2085, 2345, 2657, 2799
ZraI GACGTC 1 cut(s) 2116
ZrmI AGTACT 2 cut(s) 597, 800
Zsp2I ATGCAT 2 cut(s) 316, 1747
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.