RchiOBHm_Chr5g0069411
ERF Family

Belongs to the ABC transporter superfamily. ABCG family. PDR (TC 3.A.1.205) subfamily

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
5
OG0007618
Physical Location & Seq
Forward (+)
75279723 .. 75287973
8251 bp
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UTR
Exon/CDS
Intron
PRQ34480

Sequence Viewer

Length: 4266 bp
ATGGCGGCCTCGAACGGGAGCGAGTACTTCGAATTGGGAATCGAGGGGCCGAACGAGACGTTCGCTCGGCCGTCGAACGCCGAGTCGGTGGCGGAGGACGAGGAGGAGCTGATGTGGGCGGCGATCAAGCGGCTGCCGTCGCAGAAGCAGTCGAGCACCGCCATTCTCAAGTCCGACAAGTCGGAGACCATCGACGTCCGGAAACTCGATCGCCACAACCGCGAGCTCGTCGTCAGCAAAGCCCTCGCCACTAACGATCAGGACAACCACCGCCTCCTCTGCGCCATTAAAGAACGCCTCGACAGAGTGGGATTGGAGGTTCGAAAAGTGGAAGTGCGGTACGAGAACCTGAATATCGTGGCAGACGTTCAAACAGGATCAAGAGCTTTGCCTACTCTCATCAACTATACTCGGGATGGTATAGAGGGTTTTTTAACTAGTTGTAGGATTTTTAAACCCAAGAGACGGTCTTTAAAGATTTTGAACAACGTCAGCGGTGTTATCAAACCCGGAAGGATGACTTTGCTTTTAGGACCCCCAAGTTCTGGCAAATCCACTTTGCTCACGACTCTTGCAGGAAAACTGGACTCCAGCTTAAAGAAAAGTGGTAGCATCACCTACAATGGGCATAATCTTGATGAGTTCTGCGTTCAAAGAACTGCTGCTTACATAAGCCAAACAGATAATCATATTGCAGAACTCACCGTGAGAGAAACCTTAGACTTTGCAGCTAGATGTCAAGGTGCAAGCGAAGGTTTTGCAGCGTACATGAAAAATCTGGCCAAGTTAGAGAAGGAAAAAAACATAAGGCCAGATCCAGTAATTGATGCATTCATGAAGGCATCATCTGTTGGTGGTAAAAAGCACAGTATTACAACAGATTATGTTCTCAAATTGCTTGGTCTTGATGTTTGTTCAGATACAATTGTTGGAAACGACATGTTAAGAGGGGTTTCTGGGGGACAAAGGAAAAGGGTTACTACAGGTGAAATGGCAGTTGGACCAAGAAAAACCCTGTTTATGGATGAAATATCTACTGGATTGGATAGTTCTACTACATACCAAATTGTAAAATGTATTGGGAATTTTGTTCATCACATGGAAGCTACAGTACTAATGGCTCTGCTTCAGCCTGCACCTGAGACATTTGAATTGTTTGATGATTTAGTGCTACTATCTGAAGGCTACGTGGTTTATCAAGGCCCTCGAGCAGAAGTGTTGGAGTTCTTCGAGTCATTAGGCTTCAAACTACCACCACGAAAGGGTGTTGCAGATTTTCTTCAAGAGGTAACGTCTAAAAAAGATCAAGCTCAGTACTGGGCTGATCATTCGAAACCGTACGCATACATTTCTGTTTCAGAAATTGCAGAAGCCTTTAAGAAGTCCAGGTTTGGAAGGTCTGTGGATGACACTCTCTCTGCTCCATATGATAAATCTAGCAGTCTTCCTGCGGCTTTGCCAAAAACTAAATATGCAACCTCAAAATGGGAGCTTTGTAAAGCATGCTTTTCACGAGAACTACTGTTGATCAGCAGGCATAGGTTTCTTTACACATTTAGGACCTGCCAGGTTGCATTTGTTGGGGCTGTGACATGTACAATGTTTTCTAGATTGAAATTACACCCGACAGATGAAATAAATGGCAACCTTTATCTTTCTTGCTTGTTCTTTGGGTTGGTGCACATGATGTTTAATGGGTTTTCCGAGCTACCTCTTATGATATCTCGGCTTCCAGTCTTTTACAAGCAAAGAGACAATCACTTTCATCCTGCATGGGCATGGTCTGTTGTTAGTTTGCTTCTGCGCATACCTTACTCTATTATCGAAGCTGTTGTATGGTCTTGTGTCGTATACTACTCGGTTGGATTTGCCCCTGGTGCTGGGAGATTTTTCCGCTTCATGTTTTTGCTGTTCTCTGTGCACCAAATGGCTTTGGGTCTCTTCCGGACAATGGCATCTCTTGCAAGAGATATGGTCATTGCCAATACATTTGGATCAGCTGCACTAATTATTATATTTTCTTTTGGTGGATTCATCATTCCCAAAGCTAGCATTAAGCCATGGTGGGAATGGGCCTTTTGGATATCACCACTATCATATGGACAACGTGCTCTTTCTGTCAATGAATTTACGGCTGTAAGGTGGATCAAGATATCTCCTCTAACTAACAGAACAATTGGGCAAAGTGTTCTCCAAGAACACAACTTACCAAATGGCGATTACTGGTATTGGATTGGAGTTGGTGCTCTGTTAGCTTATGCATTGCTTTTCAATTGCATAGTGACTCTGACCTTGATGTACCTAAATCCACTAAGGAAAAATCAGACAGTGCTTCCACTTGATGACACACAAGAGAGTCCTGCTGCAGATGGGGGTAAATATAAGACCGAATCTATCCCAAAATCAACTGGAGATAACGGCCCAAGAAAGGGAATGATCTTACCATTTCAGCCATTAACAATGACTTTCCACAATGTTAACTACTCTGTTGACATGCCAAAGGAAATGAAGTCACAAGGTATACCAGAATCGAAGTTGAAACTCTTGTCGGAGGTGAGTGGAGTATTCTCACCAGGTGTTCTTACAGCTTTAGTTGGGTCTAGTGGAGCTGGAAAGACCACTTTGATGGACGTCCTTGCTGGTAGGAAAACTGGTGGATACATAGAAGGAGATATCAAGATATCTGGTTACCCGAAAGAGCAACGTACTTTTGCAAGAATAGCAGGATATGTTGAGCAAAATGACATACATTCTCCTCAACTTACAGTTGAAGAGTCTCTATGGTTTTCTTCTGCTCTTCGCCTTCCGAAGGAAGTCAGCACAGAGAAAAGACTTGAGTTTGTCGAAGAAGTAATGAAACTAGTAGAGCTTGATACTCTAAGGCATGCTTTGGTTGGTTTGCCAGGCAGTTCAGGCTTATCAACAGAGCAAAGAAAACGCTTAACAATTTCTGTTGAGCTTGTTGCCAACCCTTCAATTATCTTTATGGATGAACCCACATCTGGACTTGATGCAAGGGCAGCAGCCATTGTGATGAGGACTGTTCGTAATACAGTTGATACAGGAAGAACGGTGGTCTGCACTATACATCAACCAAGTATTGACATATTTGAGGCATTTGATGAGCTTCTTCTTATGAAACGAGGGGGGCAAGTTATTTATGGAGGAAACCTAGGCCAGCACTCCCAGACAATGATAAACTACTTTCAGGAGATCAATGGAATCACTCCGATTCCAAGCGGGTACAATCCAGCAACCTGGATGCTGGAGGTAACCACACCGGCTTGTGAACAGAGAATTGGGGAAGACTTCGCTAGCATATACAGAAAATCAGAGCAGTACAGGGAGGTGGAAGAGTCCATCAAGCAGTTTAGCACTCCTCCAGCTGACTCAAAACCATTAGAGTTTGCTTCCACGTATTCACAAAACACATTGGCTCAATTTTGGACCTGCTTATGGAAACAAAATCTTGTGTATTGGAGAAGTCCACAATATAATGCCATGAGGTTAATCTTTACTACGCTTGCTGCATTGATATTCGGTTCAGCATTTTGGGATGTTGGTACAAAAAGGGACTCAATTCAATCATTGATGATGGTTATGGGAGCTCTTTATTCTGCTTGCCTGTTTCTTGGGGTCAATAACGCTTCTTCAGTTCAGCCAATTGTTTCAATTGAGAGGACAGTATTTTATCGAGAGAAAGCAGCTGGAATGTATTCTCCTTTCGCTTATGCAGCGGCCCAGGGCCTTATAGAGATCCCATACATTGCTGTACAGACAATAGTATACGGGGTGATCACATACTTCATGGTCAATTTTGAAAGGACAATCACAAAATTCTTGCTCTATATTCTGTTCATGTTCCTCACATTCACCTACTTCACCCTTTATGGCATGGCGGCTGTTGGTCTCACTCCCTCTCAACACCTGGCAGCTGTCATCTCTTCTGCATTTTACTCATTGTGGAATCTCCTCGCCGGTTTCCTCATCCCCAAACCAAACATCCCGCCATGGTGGATATGGTTTTACTACATCACCCCAGTTGCATGGACACTTAGAGGTATTATCACTTCCCAACTTGGTGATGTGGAGACCATTCTTGTCGGACCGGCGTTTAAGGGCACTGTGAAAGAATACTTGGAAGTCAGCCTTGGCTATGGCCCTGGAATGATTGGAGTTTCAGTAGCTGTGCTACTGGGTTTCAACGTCCTTTTCTTCAGTATCTTTGCCTTCTCCATCAAATTTCTTAACTTCCAGAGAAGATAG

Protein Analysis

1421

Amino Acids

158.78

Weight (kDa)

8.67

Isoelectric Point (pI)

35.65

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
ABC_trans_N PF14510 85 - 135 2.2e-06 ABC-transporter N-terminal
ABC_tran PF00005 161 - 343 4.1e-17 ABC transporter
ABC2_membrane_7 PF19055 377 - 427 4.7e-06 ABC-2 type transporter
ABC2_membrane PF01061 498 - 710 1.7e-48 ABC-2 type transporter
PDR_assoc PF08370 715 - 777 9.8e-23 Plant PDR ABC transporter associated
ABC_tran PF00005 850 - 1001 6.5e-20 ABC transporter
ABC2_membrane PF01061 1147 - 1359 1.2e-57 ABC-2 type transporter
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 2 cut(s) 198, 2634
Acc16I TGCGCA 1 cut(s) 1805
Acc36I ACCTGC 2 cut(s) 1571, 3458
AccB7I CCANNNNNTGG 1 cut(s) 545
AccI GTMKAC 3 cut(s) 1851, 2521, 3786
AccII CGCG 1 cut(s) 222
AccIII TCCGGA 2 cut(s) 198, 1944
AclWI GGATC 5 cut(s) 385, 809, 2002, 2153, 3751
AcoI YGGCCR 2 cut(s) 68, 780
AcsI RAATTY 4 cut(s) 1084, 2126, 3836, 4241
AcuI CTGAAG 4 cut(s) 1112, 1200, 3636, 4201
AcyI GRCGYC 2 cut(s) 195, 2631
AdeI CACNNNGTG 1 cut(s) 2576
AflIII ACRYGT 2 cut(s) 939, 1592
AhlI ACTAGT 2 cut(s) 437, 2860
AjnI CCWGG 9 cut(s) 1385, 1566, 1873, 2572, 2902, 3257, 3741, 3927, 4162
AjuI GAANNNNNNNTTGG 8 cut(s) 981, 1013, 3282, 3314, 4121, 4134, 4153, 4166
AloI GAACNNNNNNTCC 2 cut(s) 303, 335
Alw21I GWGCWC 7 cut(s) 158, 228, 1683, 1923, 2113, 2248, 3610
Alw26I GTCTC 9 cut(s) 50, 179, 457, 1136, 1746, 1943, 2781, 3914, 4085
Alw44I GTGCAC 2 cut(s) 1679, 1919
AlwI GGATC 5 cut(s) 385, 809, 2002, 2153, 3751
AlwNI CAGNNNCTG 1 cut(s) 4187
Ama87I CYCGRG 2 cut(s) 411, 1206
Aor13HI TCCGGA 2 cut(s) 198, 1944
ApaLI GTGCAC 2 cut(s) 1679, 1919
ApoI RAATTY 4 cut(s) 1084, 2126, 3836, 4241
ArsI GACNNNNNNTTYG 2 cut(s) 68, 100
Asp700I GAANNNNTTC 1 cut(s) 3715
AspA2I CCTAGG 1 cut(s) 3172
AspLEI GCGC 2 cut(s) 284, 1806
AsuC2I CCSGG 1 cut(s) 510
AsuII TTCGAA 3 cut(s) 30, 322, 1331
AsuNHI GCTAGC 2 cut(s) 2048, 3314
AvaI CYCGRG 2 cut(s) 411, 1206
AvaII GGWCC 5 cut(s) 533, 1001, 1560, 3447, 4106
AvrII CCTAGG 1 cut(s) 3172
BaeGI GKGCMC 3 cut(s) 1683, 1923, 4124
BaeI ACNNNNGTAYC 2 cut(s) 912, 945
BalI TGGCCA 1 cut(s) 782
BanII GRGCYC 2 cut(s) 228, 3610
BarI GAAGNNNNNNTAC 4 cut(s) 1095, 1127, 4054, 4086
BauI CACGAG 1 cut(s) 1512
BbsI GAAGAC 2 cut(s) 1436, 3312
Bbv12I GWGCWC 7 cut(s) 158, 228, 1683, 1923, 2113, 2248, 3610
BccI CCATC 7 cut(s) 197, 410, 2363, 2620, 3368, 3589, 4244
BceAI ACGGC 4 cut(s) 55, 121, 2148, 2434
BcgI CGANNNNNNTGC 4 cut(s) 226, 260, 740, 774
BciT130I CCWGG 9 cut(s) 1387, 1568, 1875, 2574, 2904, 3259, 3743, 3929, 4164
BciVI GTATCC 1 cut(s) 2651
BclI TGATCA 3 cut(s) 1324, 1527, 3795
BcnI CCSGG 1 cut(s) 510
BcoDI GTCTC 9 cut(s) 50, 179, 457, 1136, 1746, 1943, 2781, 3914, 4085
BcuI ACTAGT 2 cut(s) 437, 2860
BfaI CTAG 9 cut(s) 438, 732, 1437, 1608, 2049, 2601, 2861, 3173, 3315
BfmI CTRYAG 3 cut(s) 981, 1107, 2364
BfuAI ACCTGC 2 cut(s) 1571, 3458
BfuI GTATCC 1 cut(s) 2651
BlnI CCTAGG 1 cut(s) 3172
BmcAI AGTACT 3 cut(s) 26, 1113, 1316
Bme18I GGWCC 5 cut(s) 533, 1001, 1560, 3447, 4106
BmeT110I CYCGRG 2 cut(s) 411, 1206
BmiI GGNNCC 2 cut(s) 48, 535
BmrI ACTGGG 3 cut(s) 1327, 4034, 4205
BmsI GCATC 6 cut(s) 621, 817, 851, 1964, 3001, 3252
BmtI GCTAGC 2 cut(s) 2052, 3318
BmuI ACTGGG 3 cut(s) 1327, 4034, 4205
BpiI GAAGAC 2 cut(s) 1436, 3312
BpmI CTGGAG 4 cut(s) 574, 2430, 3287, 3366
Bpu14I TTCGAA 3 cut(s) 30, 322, 1331
BpuEI CTTGAG 2 cut(s) 152, 2855
BpuMI CCSGG 1 cut(s) 510
BsaAI YACGTR 2 cut(s) 1189, 3417
BsaHI GRCGYC 2 cut(s) 195, 2631
BsaI GGTCTC 4 cut(s) 179, 1943, 3914, 4085
BsaJI CCNNGG 8 cut(s) 1873, 2060, 3172, 3741, 3742, 4010, 4150, 4162
BsaWI WCCGGW 2 cut(s) 198, 1944
BsaXI ACNNNNNCTCC 4 cut(s) 98, 128, 4167, 4197
Bse118I RCCGGY 3 cut(s) 3280, 3977, 4108
Bse3DI GCAATG 3 cut(s) 1977, 2261, 3765
BseAI TCCGGA 2 cut(s) 198, 1944
BseBI CCWGG 9 cut(s) 1387, 1568, 1875, 2574, 2904, 3259, 3743, 3929, 4164
BseDI CCNNGG 8 cut(s) 1873, 2060, 3172, 3741, 3742, 4010, 4150, 4162
BseMI GCAATG 3 cut(s) 1977, 2261, 3765
BseMII CTCAG 2 cut(s) 1131, 1325
BseRI GAGGAG 7 cut(s) 116, 119, 266, 2148, 2745, 3369, 3962
BseSI GKGCMC 3 cut(s) 1683, 1923, 4124
BseX3I CGGCCG 1 cut(s) 68
BseYI CCCAGC 1 cut(s) 1880
BsgI GTGCAG 3 cut(s) 1119, 1986, 3064
Bsh1236I CGCG 1 cut(s) 222
Bsh1285I CGRYCG 2 cut(s) 71, 211
BsiEI CGRYCG 2 cut(s) 71, 211
BsiHKAI GWGCWC 7 cut(s) 158, 228, 1683, 1923, 2113, 2248, 3610
BsiHKCI CYCGRG 2 cut(s) 411, 1206
BsiSI CCGG 6 cut(s) 199, 510, 1945, 3281, 3978, 4109
BsiWI CGTACG 1 cut(s) 1338
BslFI GGGAC 2 cut(s) 975, 3587
BsmAI GTCTC 9 cut(s) 50, 179, 457, 1136, 1746, 1943, 2781, 3914, 4085
BsmBI CGTCTC 2 cut(s) 50, 457
BsmFI GGGAC 2 cut(s) 975, 3587
BsmI GAATGC 1 cut(s) 830
Bso31I GGTCTC 4 cut(s) 179, 1943, 3914, 4085
BsoBI CYCGRG 2 cut(s) 411, 1206
Bsp119I TTCGAA 3 cut(s) 30, 322, 1331
Bsp1286I GDGCHC 8 cut(s) 158, 228, 1683, 1923, 2113, 2248, 3610, 4124
Bsp13I TCCGGA 2 cut(s) 198, 1944
Bsp1407I TGTACA 2 cut(s) 1595, 3772
Bsp19I CCATGG 2 cut(s) 2060, 4010
BspCNI CTCAG 2 cut(s) 1132, 1324
BspEI TCCGGA 2 cut(s) 198, 1944
BspFNI CGCG 1 cut(s) 222
BspHI TCATGA 1 cut(s) 834
BspLI GGNNCC 2 cut(s) 48, 535
BspMAI CTGCAG 1 cut(s) 2368
BspMI ACCTGC 2 cut(s) 1571, 3458
BspOI GCTAGC 2 cut(s) 2052, 3318
BspPI GGATC 5 cut(s) 385, 809, 2002, 2153, 3751
BspQI GCTCTTC 1 cut(s) 2802
BspT104I TTCGAA 3 cut(s) 30, 322, 1331
BspTNI GGTCTC 4 cut(s) 179, 1943, 3914, 4085
BsrDI GCAATG 3 cut(s) 1977, 2261, 3765
BsrFI RCCGGY 3 cut(s) 3280, 3977, 4108
BsrGI TGTACA 2 cut(s) 1595, 3772
BssAI RCCGGY 3 cut(s) 3280, 3977, 4108
BssECI CCNNGG 8 cut(s) 1873, 2060, 3172, 3741, 3742, 4010, 4150, 4162
BssNAI GTATAC 3 cut(s) 1852, 2522, 3787
BssNI GRCGYC 2 cut(s) 195, 2631
BssSI CACGAG 1 cut(s) 1512
BssT1I CCWWGG 4 cut(s) 2060, 3172, 4010, 4150
Bst1107I GTATAC 3 cut(s) 1852, 2522, 3787
Bst2BI CACGAG 1 cut(s) 1512
Bst2UI CCWGG 9 cut(s) 1387, 1568, 1875, 2574, 2904, 3259, 3743, 3929, 4164
Bst6I CTCTTC 5 cut(s) 1946, 2766, 2802, 3348, 3949
BstACI GRCGYC 2 cut(s) 195, 2631
BstAPI GCANNNNNTGC 1 cut(s) 1961
BstAUI TGTACA 2 cut(s) 1595, 3772
BstBAI YACGTR 2 cut(s) 1189, 3417
BstBI TTCGAA 3 cut(s) 30, 322, 1331
BstDEI CTNAG 6 cut(s) 718, 1140, 1311, 2312, 2879, 4055
BstDSI CCRYGG 2 cut(s) 2060, 4010
BstEII GGTNACC 2 cut(s) 2687, 3271
BstENI CCTNNNNNAGG 1 cut(s) 2807
BstFNI CGCG 1 cut(s) 222
BstHHI GCGC 2 cut(s) 284, 1806
BstMAI GTCTC 9 cut(s) 50, 179, 457, 1136, 1746, 1943, 2781, 3914, 4085
BstMCI CGRYCG 2 cut(s) 71, 211
BstNI CCWGG 9 cut(s) 1387, 1568, 1875, 2574, 2904, 3259, 3743, 3929, 4164
BstNSI RCATGY 5 cut(s) 943, 1506, 1596, 2497, 2888
BstPI GGTNACC 2 cut(s) 2687, 3271
BstSFI CTRYAG 3 cut(s) 981, 1107, 2364
BstSLI GKGCMC 3 cut(s) 1683, 1923, 4124
BstUI CGCG 1 cut(s) 222
BstV2I GAAGAC 2 cut(s) 1436, 3312
BstX2I RGATCY 2 cut(s) 814, 3756
BstXI CCANNNNNNTGG 3 cut(s) 2626, 3258, 4047
BstYI RGATCY 2 cut(s) 814, 3756
BstZ17I GTATAC 3 cut(s) 1852, 2522, 3787
BstZI CGGCCG 1 cut(s) 68
BsuI GTATCC 1 cut(s) 2651
BtgI CCRYGG 2 cut(s) 2060, 4010
BtsIMutI CAGTG 2 cut(s) 2334, 4122
BveI ACCTGC 2 cut(s) 1571, 3458
CaiI CAGNNNCTG 1 cut(s) 4187
CciI TCATGA 1 cut(s) 834
CfoI GCGC 2 cut(s) 284, 1806
Cfr10I RCCGGY 3 cut(s) 3280, 3977, 4108
CpoI CGGWCCG 1 cut(s) 4106
CsiI ACCWGGT 1 cut(s) 2572
CspI CGGWCCG 1 cut(s) 4106
DdeI CTNAG 6 cut(s) 718, 1140, 1311, 2312, 2879, 4055
DraI TTTAAA 2 cut(s) 454, 474
DraIII CACNNNGTG 1 cut(s) 2576
EaeI YGGCCR 2 cut(s) 68, 780
EagI CGGCCG 1 cut(s) 68
Eam1104I CTCTTC 5 cut(s) 1946, 2766, 2802, 3348, 3949
EarI CTCTTC 5 cut(s) 1946, 2766, 2802, 3348, 3949
EciI GGCGGA 1 cut(s) 107
Ecl136II GAGCTC 2 cut(s) 226, 3608
EclXI CGGCCG 1 cut(s) 68
Eco130I CCWWGG 4 cut(s) 2060, 3172, 4010, 4150
Eco24I GRGCYC 2 cut(s) 228, 3610
Eco31I GGTCTC 4 cut(s) 179, 1943, 3914, 4085
Eco32I GATATC 5 cut(s) 1722, 2085, 2154, 2674, 2682
Eco47I GGWCC 5 cut(s) 533, 1001, 1560, 3447, 4106
Eco52I CGGCCG 1 cut(s) 68
Eco53kI GAGCTC 2 cut(s) 226, 3608
Eco57I CTGAAG 4 cut(s) 1112, 1200, 3636, 4201
Eco88I CYCGRG 2 cut(s) 411, 1206
Eco91I GGTNACC 2 cut(s) 2687, 3271
EcoICRI GAGCTC 2 cut(s) 226, 3608
EcoNI CCTNNNNNAGG 1 cut(s) 2807
EcoO109I RGGNCCY 4 cut(s) 533, 1202, 1560, 3745
EcoO65I GGTNACC 2 cut(s) 2687, 3271
EcoRII CCWGG 9 cut(s) 1385, 1566, 1873, 2572, 2902, 3257, 3741, 3927, 4162
EcoRV GATATC 5 cut(s) 1722, 2085, 2154, 2674, 2682
EcoT14I CCWWGG 4 cut(s) 2060, 3172, 4010, 4150
EcoT22I ATGCAT 2 cut(s) 832, 2263
EcoT38I GRGCYC 2 cut(s) 228, 3610
ErhI CCWWGG 4 cut(s) 2060, 3172, 4010, 4150
Esp3I CGTCTC 2 cut(s) 50, 457
FalI AAGNNNNNCTT 6 cut(s) 505, 537, 2872, 2904, 4134, 4166
FaqI GGGAC 2 cut(s) 975, 3587
FauI CCCGC 2 cut(s) 3233, 4014
FauNDI CATATG 2 cut(s) 1426, 2098
FbaI TGATCA 3 cut(s) 1324, 1527, 3795
FblI GTMKAC 3 cut(s) 1851, 2521, 3786
FriOI GRGCYC 2 cut(s) 228, 3610
FspBI CTAG 9 cut(s) 438, 732, 1437, 1608, 2049, 2601, 2861, 3173, 3315
FspI TGCGCA 1 cut(s) 1805
GlaI GCGC 2 cut(s) 283, 1805
GsaI CCCAGC 1 cut(s) 1884
GsuI CTGGAG 4 cut(s) 574, 2430, 3287, 3366
HapII CCGG 6 cut(s) 199, 510, 1945, 3281, 3978, 4109
HhaI GCGC 2 cut(s) 284, 1806
Hin1I GRCGYC 2 cut(s) 195, 2631
Hin6I GCGC 2 cut(s) 282, 1804
HinP1I GCGC 2 cut(s) 282, 1804
HincII GTYRAC 2 cut(s) 2479, 2491
HindII GTYRAC 2 cut(s) 2479, 2491
HpaI GTTAAC 1 cut(s) 2479
HpaII CCGG 6 cut(s) 199, 510, 1945, 3281, 3978, 4109
Hpy166II GTNNAC 9 cut(s) 1681, 1852, 1921, 2479, 2491, 2522, 3290, 3488, 3787
Hpy8I GTNNAC 9 cut(s) 1681, 1852, 1921, 2479, 2491, 2522, 3290, 3488, 3787
Hpy99I CGWCG 4 cut(s) 76, 142, 197, 233
HpyF3I CTNAG 6 cut(s) 718, 1140, 1311, 2312, 2879, 4055
Hsp92I GRCGYC 2 cut(s) 195, 2631
HspAI GCGC 2 cut(s) 282, 1804
Kpn2I TCCGGA 2 cut(s) 198, 1944
Ksp22I TGATCA 3 cut(s) 1324, 1527, 3795
KspAI GTTAAC 1 cut(s) 2479
LguI GCTCTTC 1 cut(s) 2802
LmnI GCTCC 6 cut(s) 18, 106, 1426, 1489, 2606, 3605
LweI GCATC 6 cut(s) 621, 817, 851, 1964, 3001, 3252
MabI ACCWGGT 1 cut(s) 2572
MaeI CTAG 9 cut(s) 438, 732, 1437, 1608, 2049, 2601, 2861, 3173, 3315
MaeIII GTNAC 7 cut(s) 976, 1288, 1588, 2281, 2511, 2687, 3271
MfeI CAATTG 5 cut(s) 924, 2175, 2272, 3663, 3672
MflI RGATCY 2 cut(s) 814, 3756
MhlI GDGCHC 8 cut(s) 158, 228, 1683, 1923, 2113, 2248, 3610, 4124
MlsI TGGCCA 1 cut(s) 782
MluNI TGGCCA 1 cut(s) 782
MmeI TCCRAC 8 cut(s) 162, 198, 910, 979, 1200, 1843, 2529, 4084
Mox20I TGGCCA 1 cut(s) 782
Mph1103I ATGCAT 2 cut(s) 832, 2263
MroI TCCGGA 2 cut(s) 198, 1944
MroXI GAANNNNTTC 1 cut(s) 3715
MscI TGGCCA 1 cut(s) 782
MslI CAYNNNNRTG 3 cut(s) 1777, 2624, 3032
Msp20I TGGCCA 1 cut(s) 782
MspA1I CMGCKG 6 cut(s) 495, 2000, 3386, 3707, 3737, 3935
MspI CCGG 6 cut(s) 199, 510, 1945, 3281, 3978, 4109
MunI CAATTG 5 cut(s) 924, 2175, 2272, 3663, 3672
Mva1269I GAATGC 1 cut(s) 830
MvaI CCWGG 9 cut(s) 1387, 1568, 1875, 2574, 2904, 3259, 3743, 3929, 4164
MvnI CGCG 1 cut(s) 222
NciI CCSGG 1 cut(s) 510
NcoI CCATGG 2 cut(s) 2060, 4010
NdeI CATATG 2 cut(s) 1426, 2098
NheI GCTAGC 2 cut(s) 2048, 3314
NlaIV GGNNCC 2 cut(s) 48, 535
NmeAIII GCCGAG 3 cut(s) 46, 106, 1705
NmuCI GTSAC 3 cut(s) 1588, 2281, 2511
NsbI TGCGCA 1 cut(s) 1805
NsiI ATGCAT 2 cut(s) 832, 2263
NspI RCATGY 5 cut(s) 943, 1506, 1596, 2497, 2888
NspV TTCGAA 3 cut(s) 30, 322, 1331
PaeI GCATGC 2 cut(s) 1506, 2888
PaeR7I CTCGAG 1 cut(s) 1206
PagI TCATGA 1 cut(s) 834
PasI CCCWGGG 1 cut(s) 3742
PciI ACATGT 2 cut(s) 939, 1592
PciSI GCTCTTC 1 cut(s) 2802
PcsI WCGNNNNNNNCGW 2 cut(s) 252, 363
PctI GAATGC 1 cut(s) 830
PdmI GAANNNNTTC 1 cut(s) 3715
PfeI GAWTC 7 cut(s) 39, 2031, 2390, 2528, 3222, 3232, 3967
Pfl23II CGTACG 1 cut(s) 1338
PflMI CCANNNNNTGG 1 cut(s) 545
Ple19I CGATCG 1 cut(s) 211
Ppu21I YACGTR 2 cut(s) 1189, 3417
PpuMI RGGWCCY 2 cut(s) 533, 1560
PscI ACATGT 2 cut(s) 939, 1592
Psp124BI GAGCTC 2 cut(s) 228, 3610
Psp5II RGGWCCY 2 cut(s) 533, 1560
Psp6I CCWGG 9 cut(s) 1385, 1566, 1873, 2572, 2902, 3257, 3741, 3927, 4162
PspEI GGTNACC 2 cut(s) 2687, 3271
PspFI CCCAGC 1 cut(s) 1880
PspGI CCWGG 9 cut(s) 1385, 1566, 1873, 2572, 2902, 3257, 3741, 3927, 4162
PspLI CGTACG 1 cut(s) 1338
PspN4I GGNNCC 2 cut(s) 48, 535
PspPPI RGGWCCY 2 cut(s) 533, 1560
PspXI VCTCGAGB 1 cut(s) 1206
PsrI GAACNNNNNNTAC 2 cut(s) 2190, 2222
PstI CTGCAG 1 cut(s) 2368
PstNI CAGNNNCTG 1 cut(s) 4187
PsuI RGATCY 2 cut(s) 814, 3756
PvuI CGATCG 1 cut(s) 211
PvuII CAGCTG 4 cut(s) 2000, 3386, 3707, 3935
RseI CAYNNNNRTG 3 cut(s) 1777, 2624, 3032
Rsr2I CGGWCCG 1 cut(s) 4106
RsrII CGGWCCG 1 cut(s) 4106
SacI GAGCTC 2 cut(s) 228, 3610
SapI GCTCTTC 1 cut(s) 2802
ScaI AGTACT 3 cut(s) 26, 1113, 1316
SduI GDGCHC 8 cut(s) 158, 228, 1683, 1923, 2113, 2248, 3610, 4124
SexAI ACCWGGT 1 cut(s) 2572
SfaNI GCATC 6 cut(s) 621, 817, 851, 1964, 3001, 3252
SfcI CTRYAG 3 cut(s) 981, 1107, 2364
Sfr274I CTCGAG 1 cut(s) 1206
SfuI TTCGAA 3 cut(s) 30, 322, 1331
SinI GGWCC 5 cut(s) 533, 1001, 1560, 3447, 4106
SlaI CTCGAG 1 cut(s) 1206
SmiMI CAYNNNNRTG 3 cut(s) 1777, 2624, 3032
SmlI CTYRAG 3 cut(s) 167, 1206, 2834
SmoI CTYRAG 3 cut(s) 167, 1206, 2834
SpeI ACTAGT 2 cut(s) 437, 2860
SphI GCATGC 2 cut(s) 1506, 2888
SspMI CTAG 9 cut(s) 438, 732, 1437, 1608, 2049, 2601, 2861, 3173, 3315
SstI GAGCTC 2 cut(s) 228, 3610
StyI CCWWGG 4 cut(s) 2060, 3172, 4010, 4150
TaqII GACCGA 1 cut(s) 2402
TatI WGTACW 6 cut(s) 24, 1111, 1314, 1595, 3339, 3772
TauI GCSGC 6 cut(s) 8, 122, 133, 1454, 3740, 3902
TfiI GAWTC 7 cut(s) 39, 2031, 2390, 2528, 3222, 3232, 3967
TscAI CASTG 2 cut(s) 2334, 4129
TseFI GTSAC 3 cut(s) 1588, 2281, 2511
Tsp45I GTSAC 3 cut(s) 1588, 2281, 2511
TspRI CASTG 2 cut(s) 2334, 4129
Van91I CCANNNNNTGG 1 cut(s) 545
VneI GTGCAC 2 cut(s) 1679, 1919
VpaK11BI GGWCC 5 cut(s) 533, 1001, 1560, 3447, 4106
XagI CCTNNNNNAGG 1 cut(s) 2807
XapI RAATTY 4 cut(s) 1084, 2126, 3836, 4241
XbaI TCTAGA 1 cut(s) 1607
XceI RCATGY 5 cut(s) 943, 1506, 1596, 2497, 2888
XcmI CCANNNNNNNNNTGG 2 cut(s) 2067, 4017
XhoI CTCGAG 1 cut(s) 1206
XmaJI CCTAGG 1 cut(s) 3172
XmiI GTMKAC 3 cut(s) 1851, 2521, 3786
XmnI GAANNNNTTC 1 cut(s) 3715
XspI CTAG 9 cut(s) 438, 732, 1437, 1608, 2049, 2601, 2861, 3173, 3315
ZraI GACGTC 2 cut(s) 196, 2632
ZrmI AGTACT 3 cut(s) 26, 1113, 1316
Zsp2I ATGCAT 2 cut(s) 832, 2263
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.