MD11G1095500.v1.1

Pentatricopeptide repeat-containing protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr11
OG0008107
Physical Location & Seq
Reverse (-)
7956362 .. 7958092
1731 bp
Loading structure...
UTR
Exon/CDS
Intron
MD11G1095500.v1.1.491

Sequence Viewer

Length: 1731 bp
ATGGCAGTGACCCAGAAGCTTGCAAAGCCGTTTGGTTTGGGGCTGCTGGCTCAGTTGCAACAGGGTGGGTCTTCACTTTCCCCTCAAACCCTCAACAAAATCGTATCTTCTTGCGCAAAGTCGGCTTCTTTGGATCTGGGCATTAAGATTCACGCATTTTCTATCAAGTTGGGTTTTCGTTCTAACGTCTATATATCCAGTGCTCTCGTTGATATGTATGGCAAATGCGGTTCGTTGCTGAATGCTCAGATACTGTTTGATGAAATGCCCGAGAGAAATGTTGTGACTTGGAATTCTCTGATTTCTGGTTATTTGCAAGCTGAGTTGCCAAAAAGGGCAGTTGGGTTGTTTCTGGAGATGTTGAAAGTGGGGATAGTGCCAACGCCGTTCAGTCTTTCGGGGGTCTTGGTGGGTTGCTCACAGCTAGAAGCTGAAGAGCTTGGGGCTCAGGTGCATAGTTTGAGCATGAAAATGGGGATTTGCTGCAATGTTGTTGTGGGAACTGGATTGATAGATATGTATTCGAAGTGTTGTAGTGTTAACGAATCAAGGCGTGTGTTTAATGAAATGCCAGAAAAGAATGTTATAACTTGGACTTCCATGGTTACTGGTTATGCACAGAATGGACAATCCAACGAGGCGATGATTTTAGCGAGGGAAATGTTGCATTCGGGTCACAAACCAAATTATGTAACTTATAATAGCTTGTTGAGTTCATTTTCGAGCCCAGACTTTTGGAATCATTGCAGGCAAATCCATTGCCGGATAATGAAAGAAGGTTTTGAGTTTAATGTGTATATAGTGGTTACACTTATGACCGTATATTCAGAATGTTGCAATAGCTTGGAAGACTTTCAAAAGCTTTGCTCGTGCGTTGCAGTATGGGATCAAATCTCATGGAATGGAGTAATCGCTGGTTTTTCTAATATTGGAAACAGTGAGGAAGCTCTGCAGTGTTTCTCTGAAATGAGGCAGGCCGGAGTTTCTATGAACTTCTTCACATTTGCAAGCATTTTAAGCGCGGTAGGAACTCTTTCAGCCCTTGAAGAGGGACAGAAAATCCATGCTCTCATTTTCAAGAGTGGGCATGCTTCAAATTTATGTGTTCAGAATGGGCTTGTTTCAATGTACGCAAGATGCGGTGCCATCCATGATTCGAAGCGAGTGTTTAATTTGATGAACGAACATGATGTCATCTCCTGGAATTCATTATTATCAGGTTATGCTCATCATGGATTTGGGTTGGAGGCTGTTGAACTGTTTGAGGAAATGAGACGAACAGATGTCAAACCAGATAGTACCGCCTTCCTTATTGTACTAACTGCTTGTAGCCATGTTGGTTTAGTGGACAAAGGATTCGAGTATTTCAATCTGATGAGAAATGATGATTTTTGTGCACCCCCGAAAATGGAGCATTATGCAACAGTTGTTGACCTTTTCGGTCGAGCTGGAAATCTTCATGAAGCACAAGCCTTTGTCGATAACATGCCAATCGAGGCGGGACCATCAGTGTACAAAGCTCTACTTAGTGCTTGCCAAGTTCATGGAAATAAGGAAATCGCTCTTCGTTCGGCACAAAAGCTTCAGGAACTGTCCCCAAATGATCCCGCAACTTACATACTCCTGTCGAACATGTTGCTGACAAGAGGCTACTGGGATGATGCAGCAGGAGTAAGGAAGCTCATGTATGATAGAGGAATAAGAAAAACTCCCGGTTACAGTTGGATTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
PPR PPR_2 Pkinase_Tyr

Protein Analysis

577

Amino Acids

63.55

Weight (kDa)

6.25

Isoelectric Point (pI)

36.2

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PPR_2 PF13041 92 - 140 3.2e-09 PPR repeat family
PPR PF01535 95 - 125 3.7e-07 PPR repeat
PPR_2 PF13041 193 - 240 7.1e-12 PPR repeat family
PPR_3 PF13812 193 - 240 1.7e-06 Pentatricopeptide repeat domain
PPR_2 PF13041 296 - 341 1.6e-08 PPR repeat family
PPR_2 PF13041 397 - 444 1.4e-10 PPR repeat family
PPR PF01535 399 - 426 5.1e-08 PPR repeat
E_motif PF20431 515 - 576 2.4e-15 E motif
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 2 cut(s) 587, 699
Acc16I TGCGCA 1 cut(s) 115
AccB1I GGYRCC 1 cut(s) 1142
AccII CGCG 1 cut(s) 1022
AciI CCGC 6 cut(s) 228, 1022, 1140, 1302, 1499, 1608
AclWI GGATC 3 cut(s) 141, 894, 1598
AcsI RAATTY 3 cut(s) 292, 1096, 1204
AcuI CTGAAG 2 cut(s) 453, 1568
AfaI GTAC 4 cut(s) 1130, 1300, 1317, 1514
AfiI CCNNNNNNNGG 2 cut(s) 1048, 1408
AflIII ACRYGT 1 cut(s) 1632
AgsI TTSAA 8 cut(s) 364, 857, 1046, 1078, 1095, 1125, 1256, 1369
AjnI CCWGG 1 cut(s) 1199
Alw21I GWGCWC 2 cut(s) 205, 1399
Alw26I GTCTC 1 cut(s) 1267
Alw44I GTGCAC 1 cut(s) 1395
AlwI GGATC 3 cut(s) 141, 894, 1598
AlwNI CAGNNNCTG 2 cut(s) 253, 1591
Ama87I CYCGRG 1 cut(s) 269
AoxI GGCC 1 cut(s) 975
ApaLI GTGCAC 1 cut(s) 1395
ApeKI GCWGC 3 cut(s) 43, 483, 1664
ApoI RAATTY 3 cut(s) 292, 1096, 1204
ArsI GACNNNNNNTTYG 4 cut(s) 1176, 1208, 1340, 1372
Asp700I GAANNNNTTC 3 cut(s) 852, 995, 1033
AspLEI GCGC 2 cut(s) 116, 1022
AspS9I GGNCC 1 cut(s) 1502
AsuC2I CCSGG 1 cut(s) 1713
AsuII TTCGAA 2 cut(s) 524, 1157
AvaI CYCGRG 1 cut(s) 269
AvaII GGWCC 1 cut(s) 1502
BaeGI GKGCMC 1 cut(s) 1399
BanI GGYRCC 1 cut(s) 1142
BanII GRGCYC 2 cut(s) 448, 728
BauI CACGAG 1 cut(s) 868
BbsI GAAGAC 2 cut(s) 63, 855
Bbv12I GWGCWC 2 cut(s) 205, 1399
BbvI GCAGC 3 cut(s) 30, 470, 1676
BccI CCATC 2 cut(s) 1154, 1513
BceAI ACGGC 2 cut(s) 13, 370
BcgI CGANNNNNNTGC 2 cut(s) 1618, 1652
BciT130I CCWGG 1 cut(s) 1201
BcnI CCSGG 1 cut(s) 1713
BcoDI GTCTC 1 cut(s) 1267
BfaI CTAG 1 cut(s) 425
BfmI CTRYAG 1 cut(s) 950
BisI GCNGC 3 cut(s) 44, 484, 1665
BlsI GCNGC 3 cut(s) 45, 485, 1666
Bme1390I CCNGG 2 cut(s) 1201, 1713
Bme18I GGWCC 1 cut(s) 1502
BmeT110I CYCGRG 1 cut(s) 269
BmgT120I GGNCC 1 cut(s) 1502
BmiI GGNNCC 2 cut(s) 1144, 1503
BmrFI CCNGG 2 cut(s) 1201, 1713
BmrI ACTGGG 1 cut(s) 1663
BmsI GCATC 2 cut(s) 1127, 1651
BmuI ACTGGG 1 cut(s) 1663
BpiI GAAGAC 2 cut(s) 63, 855
BpmI CTGGAG 1 cut(s) 374
Bpu10I CCTNAGC 1 cut(s) 447
Bpu14I TTCGAA 2 cut(s) 524, 1157
BpuMI CCSGG 1 cut(s) 1713
BsaJI CCNNGG 1 cut(s) 600
Bsc4I CCNNNNNNNGG 2 cut(s) 1048, 1408
Bse1I ACTGG 4 cut(s) 198, 508, 613, 1658
Bse3DI GCAATG 3 cut(s) 493, 742, 757
BseBI CCWGG 1 cut(s) 1201
BseDI CCNNGG 1 cut(s) 600
BseGI GGATG 2 cut(s) 1146, 1663
BseLI CCNNNNNNNGG 2 cut(s) 1048, 1408
BseMI GCAATG 3 cut(s) 493, 742, 757
BseMII CTCAG 4 cut(s) 65, 260, 312, 461
BseNI ACTGG 4 cut(s) 198, 508, 613, 1658
BseSI GKGCMC 1 cut(s) 1399
BseXI GCAGC 3 cut(s) 30, 470, 1676
Bsh1236I CGCG 1 cut(s) 1022
Bsh1285I CGRYCG 1 cut(s) 1444
BshFI GGCC 1 cut(s) 977
BshNI GGYRCC 1 cut(s) 1142
BsiEI CGRYCG 1 cut(s) 1444
BsiHKAI GWGCWC 2 cut(s) 205, 1399
BsiHKCI CYCGRG 1 cut(s) 269
BsiSI CCGG 3 cut(s) 763, 978, 1713
BslFI GGGAC 3 cut(s) 1065, 1515, 1579
BslI CCNNNNNNNGG 2 cut(s) 1048, 1408
BsmAI GTCTC 1 cut(s) 1267
BsmBI CGTCTC 1 cut(s) 1267
BsmFI GGGAC 3 cut(s) 1065, 1515, 1579
BsmI GAATGC 2 cut(s) 247, 667
BsnI GGCC 1 cut(s) 977
BsoBI CYCGRG 1 cut(s) 269
Bsp119I TTCGAA 2 cut(s) 524, 1157
Bsp1286I GDGCHC 4 cut(s) 205, 448, 728, 1399
Bsp1407I TGTACA 1 cut(s) 1512
Bsp143I GATC 3 cut(s) 133, 886, 1603
Bsp19I CCATGG 1 cut(s) 600
BspACI CCGC 6 cut(s) 228, 1022, 1140, 1302, 1499, 1608
BspANI GGCC 1 cut(s) 977
BspCNI CTCAG 4 cut(s) 64, 259, 313, 460
BspFNI CGCG 1 cut(s) 1022
BspHI TCATGA 1 cut(s) 1459
BspLI GGNNCC 2 cut(s) 1144, 1503
BspMAI CTGCAG 1 cut(s) 954
BspPI GGATC 3 cut(s) 141, 894, 1598
BspQI GCTCTTC 2 cut(s) 429, 1569
BspT104I TTCGAA 2 cut(s) 524, 1157
BspT107I GGYRCC 1 cut(s) 1142
BsrDI GCAATG 3 cut(s) 493, 742, 757
BsrGI TGTACA 1 cut(s) 1512
BsrI ACTGG 4 cut(s) 198, 508, 613, 1658
BssECI CCNNGG 1 cut(s) 600
BssMI GATC 3 cut(s) 133, 886, 1603
BssSI CACGAG 1 cut(s) 868
BssT1I CCWWGG 1 cut(s) 600
Bst2BI CACGAG 1 cut(s) 868
Bst2UI CCWGG 1 cut(s) 1201
Bst4CI ACNGT 7 cut(s) 255, 820, 938, 1260, 1426, 1593, 1721
Bst6I CTCTTC 3 cut(s) 429, 1041, 1569
BstAUI TGTACA 1 cut(s) 1512
BstBI TTCGAA 2 cut(s) 524, 1157
BstC8I GCNNGC 8 cut(s) 21, 48, 318, 749, 975, 1009, 1089, 1534
BstDEI CTNAG 5 cut(s) 51, 246, 321, 447, 1526
BstDSI CCRYGG 1 cut(s) 600
BstENI CCTNNNNNAGG 1 cut(s) 1046
BstF5I GGATG 2 cut(s) 1146, 1663
BstFNI CGCG 1 cut(s) 1022
BstHHI GCGC 2 cut(s) 116, 1022
BstKTI GATC 3 cut(s) 136, 889, 1606
BstMAI GTCTC 1 cut(s) 1267
BstMBI GATC 3 cut(s) 133, 886, 1603
BstMCI CGRYCG 1 cut(s) 1444
BstMWI GCNNNNNNNGC 3 cut(s) 25, 122, 1017
BstNI CCWGG 1 cut(s) 1201
BstNSI RCATGY 3 cut(s) 1091, 1489, 1636
BstSCI CCNGG 2 cut(s) 1199, 1711
BstSFI CTRYAG 1 cut(s) 950
BstSLI GKGCMC 1 cut(s) 1399
BstUI CGCG 1 cut(s) 1022
BstV1I GCAGC 3 cut(s) 30, 470, 1676
BstV2I GAAGAC 2 cut(s) 63, 855
BstX2I RGATCY 1 cut(s) 133
BstXI CCANNNNNNTGG 2 cut(s) 735, 1544
BstYI RGATCY 1 cut(s) 133
BsuRI GGCC 1 cut(s) 977
BtgI CCRYGG 1 cut(s) 600
BtgZI GCGATG 1 cut(s) 656
BtsCI GGATG 2 cut(s) 1146, 1663
BtsI GCAGTG 2 cut(s) 12, 959
BtsIMutI CAGTG 5 cut(s) 12, 205, 943, 959, 1515
Cac8I GCNNGC 8 cut(s) 21, 48, 318, 749, 975, 1009, 1089, 1534
CaiI CAGNNNCTG 2 cut(s) 253, 1591
CciI TCATGA 1 cut(s) 1459
CfoI GCGC 2 cut(s) 116, 1022
Cfr13I GGNCC 1 cut(s) 1502
Csp6I GTAC 4 cut(s) 1129, 1299, 1316, 1513
CviQI GTAC 4 cut(s) 1129, 1299, 1316, 1513
DdeI CTNAG 5 cut(s) 51, 246, 321, 447, 1526
DpnI GATC 3 cut(s) 135, 888, 1605
DpnII GATC 3 cut(s) 133, 886, 1603
Eam1104I CTCTTC 3 cut(s) 429, 1041, 1569
EarI CTCTTC 3 cut(s) 429, 1041, 1569
Eco130I CCWWGG 1 cut(s) 600
Eco24I GRGCYC 2 cut(s) 448, 728
Eco47I GGWCC 1 cut(s) 1502
Eco57I CTGAAG 2 cut(s) 453, 1568
Eco88I CYCGRG 1 cut(s) 269
EcoNI CCTNNNNNAGG 1 cut(s) 1046
EcoRI GAATTC 2 cut(s) 292, 1204
EcoRII CCWGG 1 cut(s) 1199
EcoT14I CCWWGG 1 cut(s) 600
EcoT38I GRGCYC 2 cut(s) 448, 728
ErhI CCWWGG 1 cut(s) 600
Esp3I CGTCTC 1 cut(s) 1267
FalI AAGNNNNNCTT 2 cut(s) 1509, 1541
FaqI GGGAC 3 cut(s) 1065, 1515, 1579
FauI CCCGC 2 cut(s) 1492, 1615
Fnu4HI GCNGC 3 cut(s) 44, 484, 1665
FokI GGATG 2 cut(s) 1133, 1670
FriOI GRGCYC 2 cut(s) 448, 728
Fsp4HI GCNGC 3 cut(s) 44, 484, 1665
FspBI CTAG 1 cut(s) 425
FspI TGCGCA 1 cut(s) 115
GlaI GCGC 2 cut(s) 115, 1021
GluI GCNGC 3 cut(s) 44, 484, 1665
GsuI CTGGAG 1 cut(s) 374
HaeIII GGCC 1 cut(s) 977
HapII CCGG 3 cut(s) 763, 978, 1713
HhaI GCGC 2 cut(s) 116, 1022
Hin6I GCGC 2 cut(s) 114, 1020
HinP1I GCGC 2 cut(s) 114, 1020
HincII GTYRAC 2 cut(s) 541, 1432
HindII GTYRAC 2 cut(s) 541, 1432
HindIII AAGCTT 3 cut(s) 17, 860, 1580
HinfI GANTC 5 cut(s) 148, 545, 739, 1154, 1356
HpaI GTTAAC 1 cut(s) 541
HpaII CCGG 3 cut(s) 763, 978, 1713
Hpy166II GTNNAC 5 cut(s) 541, 1348, 1397, 1432, 1513
Hpy188I TCNGA 6 cut(s) 249, 300, 829, 964, 1110, 1374
Hpy188III TCNNGA 4 cut(s) 353, 1078, 1460, 1586
Hpy8I GTNNAC 5 cut(s) 541, 1348, 1397, 1432, 1513
HpyAV CCTTC 2 cut(s) 770, 1315
HpyCH4III ACNGT 7 cut(s) 255, 820, 938, 1260, 1426, 1593, 1721
HpyCH4IV ACGT 1 cut(s) 186
HpyF10VI GCNNNNNNNGC 3 cut(s) 25, 122, 1017
HpyF3I CTNAG 5 cut(s) 51, 246, 321, 447, 1526
HpySE526I ACGT 1 cut(s) 186
HspAI GCGC 2 cut(s) 114, 1020
KspAI GTTAAC 1 cut(s) 541
Kzo9I GATC 3 cut(s) 133, 886, 1603
LguI GCTCTTC 2 cut(s) 429, 1569
LmnI GCTCC 1 cut(s) 1411
Lsp1109I GCAGC 3 cut(s) 30, 470, 1676
LweI GCATC 2 cut(s) 1127, 1651
MaeI CTAG 1 cut(s) 425
MaeII ACGT 1 cut(s) 186
MaeIII GTNAC 7 cut(s) 7, 283, 604, 674, 691, 805, 1715
MalI GATC 3 cut(s) 135, 888, 1605
MboI GATC 3 cut(s) 133, 886, 1603
MboII GAAGA 8 cut(s) 63, 99, 446, 860, 988, 1058, 1448, 1556
MflI RGATCY 1 cut(s) 133
MhlI GDGCHC 4 cut(s) 205, 448, 728, 1399
MluCI AATT 5 cut(s) 292, 685, 1096, 1171, 1204
MmeI TCCRAC 3 cut(s) 657, 1224, 1703
MroXI GAANNNNTTC 3 cut(s) 852, 995, 1033
MseI TTAA 6 cut(s) 144, 540, 561, 789, 1016, 1170
MslI CAYNNNNRTG 1 cut(s) 470
MspI CCGG 3 cut(s) 763, 978, 1713
MspR9I CCNGG 2 cut(s) 1201, 1713
Mva1269I GAATGC 2 cut(s) 247, 667
MvaI CCWGG 1 cut(s) 1201
MvnI CGCG 1 cut(s) 1022
MwoI GCNNNNNNNGC 3 cut(s) 25, 122, 1017
NciI CCSGG 1 cut(s) 1713
NcoI CCATGG 1 cut(s) 600
NdeII GATC 3 cut(s) 133, 886, 1603
NlaIV GGNNCC 2 cut(s) 1144, 1503
NmuCI GTSAC 3 cut(s) 7, 283, 674
NsbI TGCGCA 1 cut(s) 115
NspI RCATGY 3 cut(s) 1091, 1489, 1636
NspV TTCGAA 2 cut(s) 524, 1157
PaeI GCATGC 1 cut(s) 1091
PagI TCATGA 1 cut(s) 1459
PciI ACATGT 1 cut(s) 1632
PciSI GCTCTTC 2 cut(s) 429, 1569
PctI GAATGC 2 cut(s) 247, 667
PdmI GAANNNNTTC 3 cut(s) 852, 995, 1033
PfeI GAWTC 5 cut(s) 148, 545, 739, 1154, 1356
PfoI TCCNGGA 1 cut(s) 1199
PkrI GCNGC 3 cut(s) 45, 485, 1666
PscI ACATGT 1 cut(s) 1632
PsiI TTATAA 2 cut(s) 587, 699
Psp6I CCWGG 1 cut(s) 1199
PspGI CCWGG 1 cut(s) 1199
PspN4I GGNNCC 2 cut(s) 1144, 1503
PspPI GGNCC 1 cut(s) 1502
PstI CTGCAG 1 cut(s) 954
PstNI CAGNNNCTG 2 cut(s) 253, 1591
PsuI RGATCY 1 cut(s) 133
RsaI GTAC 4 cut(s) 1130, 1300, 1317, 1514
RsaNI GTAC 4 cut(s) 1129, 1299, 1316, 1513
RseI CAYNNNNRTG 1 cut(s) 470
SapI GCTCTTC 2 cut(s) 429, 1569
SaqAI TTAA 6 cut(s) 144, 540, 561, 789, 1016, 1170
SatI GCNGC 3 cut(s) 44, 484, 1665
Sau3AI GATC 3 cut(s) 133, 886, 1603
Sau96I GGNCC 1 cut(s) 1502
ScrFI CCNGG 2 cut(s) 1201, 1713
SduI GDGCHC 4 cut(s) 205, 448, 728, 1399
SfaNI GCATC 2 cut(s) 1127, 1651
SfcI CTRYAG 1 cut(s) 950
SfuI TTCGAA 2 cut(s) 524, 1157
SinI GGWCC 1 cut(s) 1502
SmiMI CAYNNNNRTG 1 cut(s) 470
SphI GCATGC 1 cut(s) 1091
Sse9I AATT 5 cut(s) 292, 685, 1096, 1171, 1204
SsiI CCGC 6 cut(s) 228, 1022, 1140, 1302, 1499, 1608
SspI AATATT 1 cut(s) 928
SspMI CTAG 1 cut(s) 425
StyD4I CCNGG 2 cut(s) 1199, 1711
StyI CCWWGG 1 cut(s) 600
TaaI ACNGT 7 cut(s) 255, 820, 938, 1260, 1426, 1593, 1721
TaiI ACGT 1 cut(s) 189
TaqI TCGA 8 cut(s) 524, 722, 1157, 1359, 1444, 1479, 1494, 1628
TaqII GACCGA 1 cut(s) 1430
TasI AATT 5 cut(s) 292, 685, 1096, 1171, 1204
TatI WGTACW 2 cut(s) 1315, 1512
TfiI GAWTC 5 cut(s) 148, 545, 739, 1154, 1356
Tru1I TTAA 6 cut(s) 144, 540, 561, 789, 1016, 1170
Tru9I TTAA 6 cut(s) 144, 540, 561, 789, 1016, 1170
TscAI CASTG 5 cut(s) 12, 205, 943, 959, 1515
TseFI GTSAC 3 cut(s) 7, 283, 674
TseI GCWGC 3 cut(s) 43, 483, 1664
Tsp45I GTSAC 3 cut(s) 7, 283, 674
TspRI CASTG 5 cut(s) 12, 205, 943, 959, 1515
VneI GTGCAC 1 cut(s) 1395
VpaK11BI GGWCC 1 cut(s) 1502
XagI CCTNNNNNAGG 1 cut(s) 1046
XapI RAATTY 3 cut(s) 292, 1096, 1204
XceI RCATGY 3 cut(s) 1091, 1489, 1636
XmnI GAANNNNTTC 3 cut(s) 852, 995, 1033
XspI CTAG 1 cut(s) 425
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.