Rroxscaffold_2G00123910

Pentatricopeptide repeat-containing protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000002
OG0008107
Physical Location & Seq
Forward (+)
57854986 .. 57866373
11388 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_2G00123910.1

Sequence Viewer

Length: 468 bp
ATGAAAGAACATGATGTGATCTCCCGGAATTCATTATTATCAGGTTATGCACATCATGGATTTGGGAAGGAGACTGTTGAATTGTTTGAGCAAATGAGAAGAACAAAGGTCAAACCAAATAATACCACCTTCCCGATTGTATTAACCGGTTGTAGCCATGTTGGTTTGTTGGACAAAGGGCTCGAGTATTTCAATCTACCGTCATTGATCTTTTTGGTCGGGCTGGAAACCTTCACGAAGTGGAAGCCTTCATGGTTGTCGCCGAGACCTATTTCATCATTCATAAGGCAAGCTCTTCCTGGTGTTGCTCAACGATTTAGGTTTCAAGGTGGCGGTTGCAGATGGTGGGCATTGTCTTTTCATGTGGCCATTGATGATTGTAATTTAGTGCTCAGATATTCTGAAGTTTTCAAGACAAACCCAACTCATCAGGAAGTCAGTGGCAGCAACCACTCCAGGATCAATTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
PPR PPR_2 Pkinase_Tyr

Protein Analysis

155

Amino Acids

17.68

Weight (kDa)

9.51

Isoelectric Point (pI)

30.97

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PPR_2 PF13041 5 - 52 1.3e-10 PPR repeat family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AciI CCGC 1 cut(s) 333
AclWI GGATC 1 cut(s) 467
AcoI YGGCCR 1 cut(s) 366
AcsI RAATTY 1 cut(s) 28
AcuI CTGAAG 1 cut(s) 423
AdeI CACNNNGTG 1 cut(s) 240
AgeI ACCGGT 1 cut(s) 146
AgsI TTSAA 4 cut(s) 80, 193, 326, 412
AjnI CCWGG 2 cut(s) 298, 455
AluBI AGCT 1 cut(s) 293
AluI AGCT 1 cut(s) 293
Alw21I GWGCWC 1 cut(s) 393
Alw26I GTCTC 2 cut(s) 65, 259
AlwI GGATC 1 cut(s) 467
Ama87I CYCGRG 1 cut(s) 182
AoxI GGCC 1 cut(s) 366
ApeKI GCWGC 1 cut(s) 444
ApoI RAATTY 1 cut(s) 28
AsiGI ACCGGT 1 cut(s) 146
AsuC2I CCSGG 1 cut(s) 25
AvaI CYCGRG 1 cut(s) 182
BalI TGGCCA 1 cut(s) 368
BanII GRGCYC 1 cut(s) 183
Bbv12I GWGCWC 1 cut(s) 393
BbvI GCAGC 1 cut(s) 456
BccI CCATC 1 cut(s) 336
BciT130I CCWGG 2 cut(s) 300, 457
BcnI CCSGG 1 cut(s) 25
BcoDI GTCTC 2 cut(s) 65, 259
BisI GCNGC 1 cut(s) 445
BlsI GCNGC 1 cut(s) 446
Bme1390I CCNGG 3 cut(s) 25, 300, 457
BmeT110I CYCGRG 1 cut(s) 182
BmrFI CCNGG 3 cut(s) 25, 300, 457
BpmI CTGGAG 1 cut(s) 439
BpuMI CCSGG 1 cut(s) 25
BsaI GGTCTC 1 cut(s) 259
BsaWI WCCGGW 1 cut(s) 146
Bse118I RCCGGY 1 cut(s) 146
BseBI CCWGG 2 cut(s) 300, 457
BseMII CTCAG 1 cut(s) 406
BseXI GCAGC 1 cut(s) 456
BshFI GGCC 1 cut(s) 368
BshTI ACCGGT 1 cut(s) 146
BsiHKAI GWGCWC 1 cut(s) 393
BsiHKCI CYCGRG 1 cut(s) 182
BsiSI CCGG 2 cut(s) 25, 147
BsmAI GTCTC 2 cut(s) 65, 259
BsnI GGCC 1 cut(s) 368
Bso31I GGTCTC 1 cut(s) 259
BsoBI CYCGRG 1 cut(s) 182
Bsp1286I GDGCHC 2 cut(s) 183, 393
Bsp143I GATC 3 cut(s) 18, 207, 459
BspACI CCGC 1 cut(s) 333
BspANI GGCC 1 cut(s) 368
BspCNI CTCAG 1 cut(s) 405
BspPI GGATC 1 cut(s) 467
BspQI GCTCTTC 1 cut(s) 300
BspTNI GGTCTC 1 cut(s) 259
BsrFI RCCGGY 1 cut(s) 146
BssAI RCCGGY 1 cut(s) 146
BssMI GATC 3 cut(s) 18, 207, 459
Bst2UI CCWGG 2 cut(s) 300, 457
Bst4CI ACNGT 2 cut(s) 76, 201
Bst6I CTCTTC 1 cut(s) 300
BstC8I GCNNGC 1 cut(s) 291
BstDEI CTNAG 1 cut(s) 392
BstKTI GATC 3 cut(s) 21, 210, 462
BstMAI GTCTC 2 cut(s) 65, 259
BstMBI GATC 3 cut(s) 18, 207, 459
BstNI CCWGG 2 cut(s) 300, 457
BstSCI CCNGG 3 cut(s) 23, 298, 455
BstV1I GCAGC 1 cut(s) 456
BsuRI GGCC 1 cut(s) 368
BtsIMutI CAGTG 1 cut(s) 445
Cac8I GCNNGC 1 cut(s) 291
Cfr10I RCCGGY 1 cut(s) 146
CspAI ACCGGT 1 cut(s) 146
CviAII CATG 5 cut(s) 11, 56, 158, 252, 362
CviJI RGCY 6 cut(s) 156, 181, 223, 247, 293, 368
CviKI_1 RGCY 6 cut(s) 156, 181, 223, 247, 293, 368
DdeI CTNAG 1 cut(s) 392
DpnI GATC 3 cut(s) 20, 209, 461
DpnII GATC 3 cut(s) 18, 207, 459
DraIII CACNNNGTG 1 cut(s) 240
EaeI YGGCCR 1 cut(s) 366
Eam1104I CTCTTC 1 cut(s) 300
EarI CTCTTC 1 cut(s) 300
Eco24I GRGCYC 1 cut(s) 183
Eco31I GGTCTC 1 cut(s) 259
Eco57I CTGAAG 1 cut(s) 423
Eco88I CYCGRG 1 cut(s) 182
EcoRI GAATTC 1 cut(s) 28
EcoRII CCWGG 2 cut(s) 298, 455
EcoT38I GRGCYC 1 cut(s) 183
FaeI CATG 5 cut(s) 14, 59, 161, 255, 365
FaiI YATR 7 cut(s) 12, 48, 57, 159, 253, 284, 363
FatI CATG 5 cut(s) 10, 55, 157, 251, 361
Fnu4HI GCNGC 1 cut(s) 445
FriOI GRGCYC 1 cut(s) 183
Fsp4HI GCNGC 1 cut(s) 445
GluI GCNGC 1 cut(s) 445
GsuI CTGGAG 1 cut(s) 439
HaeIII GGCC 1 cut(s) 368
HapII CCGG 2 cut(s) 25, 147
Hin1II CATG 5 cut(s) 14, 59, 161, 255, 365
HpaII CCGG 2 cut(s) 25, 147
Hpy188I TCNGA 2 cut(s) 395, 403
Hpy188III TCNNGA 4 cut(s) 133, 235, 412, 431
HpyAV CCTTC 4 cut(s) 61, 139, 241, 258
HpyCH4III ACNGT 2 cut(s) 76, 201
HpyCH4V TGCA 2 cut(s) 50, 339
HpyF3I CTNAG 1 cut(s) 392
Hsp92II CATG 5 cut(s) 14, 59, 161, 255, 365
Kzo9I GATC 3 cut(s) 18, 207, 459
LguI GCTCTTC 1 cut(s) 300
LpnPI CCDG 8 cut(s) 27, 38, 160, 209, 285, 312, 416, 442
Lsp1109I GCAGC 1 cut(s) 456
MalI GATC 3 cut(s) 20, 209, 461
MboI GATC 3 cut(s) 18, 207, 459
MboII GAAGA 2 cut(s) 111, 287
MhlI GDGCHC 2 cut(s) 183, 393
MlsI TGGCCA 1 cut(s) 368
MluCI AATT 4 cut(s) 28, 80, 382, 463
MluNI TGGCCA 1 cut(s) 368
MmeI TCCRAC 1 cut(s) 150
Mox20I TGGCCA 1 cut(s) 368
MscI TGGCCA 1 cut(s) 368
MseI TTAA 1 cut(s) 143
Msp20I TGGCCA 1 cut(s) 368
MspI CCGG 2 cut(s) 25, 147
MspR9I CCNGG 3 cut(s) 25, 300, 457
MvaI CCWGG 2 cut(s) 300, 457
NciI CCSGG 1 cut(s) 25
NdeII GATC 3 cut(s) 18, 207, 459
NlaIII CATG 5 cut(s) 14, 59, 161, 255, 365
NmeAIII GCCGAG 1 cut(s) 288
PaeR7I CTCGAG 1 cut(s) 182
PciSI GCTCTTC 1 cut(s) 300
PfoI TCCNGGA 2 cut(s) 23, 455
PinAI ACCGGT 1 cut(s) 146
PkrI GCNGC 1 cut(s) 446
Psp6I CCWGG 2 cut(s) 298, 455
PspGI CCWGG 2 cut(s) 298, 455
PspXI VCTCGAGB 1 cut(s) 182
SapI GCTCTTC 1 cut(s) 300
SaqAI TTAA 1 cut(s) 143
SatI GCNGC 1 cut(s) 445
Sau3AI GATC 3 cut(s) 18, 207, 459
ScrFI CCNGG 3 cut(s) 25, 300, 457
SduI GDGCHC 2 cut(s) 183, 393
SetI ASST 8 cut(s) 46, 111, 131, 233, 271, 295, 323, 331
Sfr274I CTCGAG 1 cut(s) 182
SlaI CTCGAG 1 cut(s) 182
SmlI CTYRAG 1 cut(s) 182
SmoI CTYRAG 1 cut(s) 182
Sse9I AATT 4 cut(s) 28, 80, 382, 463
SsiI CCGC 1 cut(s) 333
StyD4I CCNGG 3 cut(s) 23, 298, 455
TaaI ACNGT 2 cut(s) 76, 201
TaqI TCGA 1 cut(s) 183
TasI AATT 4 cut(s) 28, 80, 382, 463
Tru1I TTAA 1 cut(s) 143
Tru9I TTAA 1 cut(s) 143
TscAI CASTG 1 cut(s) 445
TseI GCWGC 1 cut(s) 444
TspDTI ATGAA 6 cut(s) 17, 21, 240, 264, 271, 350
TspRI CASTG 1 cut(s) 445
XapI RAATTY 1 cut(s) 28
XhoI CTCGAG 1 cut(s) 182
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.