Rw2G022590

Pentatricopeptide repeat-containing protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr2
OG0008107
Physical Location & Seq
Reverse (-)
32294294 .. 32295893
1600 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw2G022590.1

Sequence Viewer

Length: 1548 bp
ATGTGGGTAGCGCCATTCACGCCATTGTTATCAAGTTGGGTTATCAAGTTGGGTTTTTGTTCGAATGTCTATATATGCAGTGCTCTCGTCGACGTGTACGGCAAATGTGGCAAGTCGTGGAATGCCCAGAAGGTGTTCGACGAAACGCCTCACAGAAATGTAGTGACTTGGAACTCTTTGATTTCTGGTTATTTGCAAGCTGGTTCGCCACTGAGAGCAACTCGTTTGTTTCTGGACATGTTAAGGGTCGGAACAGAGCCGCCGACGACGTTCAGCTTTTCGGGGGTGTTGGTGTGTTGCGCACAGTTAGAAGCTGAAGAGCTTGGAGCTCAGGTGCATGGTTTGAGCTTGAAAATGGGGCTTTGTGATGATGTTGTGGTGGGAACAGGATTGATTGATATGTTCTCCAAGTGTTGTAAGGTTAGAGAGTCATGGCGGGTGTTTAATCAAATGCGAGACAAGAATGTTGTGACATGGACTTCCATGGTTACTGGGTGTGCGCAGAGCGGACAGTCTGATGAGGCAATGACTTTAGTGAGAGAAATGCTGAGGTTGGGTCTCAAGCCAAATTATGTCACTTACAATAGTTTGTTGAGTTCATTTGCTAGAATGGACTTTTGGGATTGTTGCAGACAAATCCATTGCCGGATAGTGAAAGAAGGGTTTGATTCCAACGTGTATATTGTGGTTACCCTTTTGACCTTATATTCAGAGTCTAACAGTAGCTTGGAGGACTTCCGTGCGCTTTGCTCATGTGTTTCAGTGTGGGATCAAATCTCATGGAATGCAGTAATTGCAGGTTTTTGTAATATTGGGAGCAGTGAAGAAGCTCTGAAGTGTTTCTCTGAAATGAGGCAGGCCGGTGTCGCTATGGATTTTTTCACATTTACAAGCATTTTAAGAGCAGCAGGAACTCTTTCAGCACTTAATGGGCTTGTTTCAATGTATGGAAGATGCGGTGCCTTCCATGATGTAAAGTGGGTGTTTACTTTGATGAAAGAACATGATGTGGTCTCCTGGAATTCATTATTATCAGGTTATGCACATCATGGATTTGGGAAGGAGACTGTTGAATTGTTTGAGCAAATGAGAAGAACAGAGGTCAAACCAAATAATACCACCTTCCTGATTGTACTAACTGCCTGTAGCCATGTTGGTTTGTTGGACAAAGGGCTCGAGTATTTCAATCTAATGAGAAATGATGATTTGTCTGAACCACCTAAGGTGGAGCATTACGCTACCGTCGTTGATCTTTTTGGTCGGGCTGGAAACCTTCACGAAGTGGAAGCCTTCGTTAATAGCATGCCAATAGAGCCAGGTGCCTCAGTATACAAAGCTCTACTTAGTGCGTGCCAAGTTCATGGAAACAGAGAACTTGCTCTTCGTTCTGCAACAAAGCTTCAGCAACTGTGCCCAAATGATCCTGCAACTTACATACTGCTATCAAATGTGTTGTTGATAAGAGGCTCCTGGGATGATACAGCAGGAGTACGGAAGCTCATGTATGATAGAGGAATAAGAAAAACTCCTGGTTATAGTTGGATTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
PPR PPR_2 Pkinase_Tyr

Protein Analysis

515

Amino Acids

57.5

Weight (kDa)

6.43

Isoelectric Point (pI)

27.5

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PPR_2 PF13041 52 - 101 6.4e-08 PPR repeat family
PPR PF01535 55 - 84 4.9e-06 PPR repeat
PPR_long PF17177 141 - 223 3e-06 Pentacotripeptide-repeat region of PRORP
PPR_2 PF13041 154 - 203 5e-13 PPR repeat family
PPR_3 PF13812 155 - 201 4.3e-08 Pentatricopeptide repeat domain
PPR PF01535 157 - 187 1e-07 PPR repeat
PPR_2 PF13041 257 - 302 2.2e-11 PPR repeat family
PPR PF01535 259 - 289 3.1e-07 PPR repeat
PPR_2 PF13041 336 - 383 6.9e-12 PPR repeat family
PPR PF01535 338 - 365 1.7e-06 PPR repeat
E_motif PF20431 454 - 515 6.8e-13 E motif
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc16I TGCGCA 2 cut(s) 301, 501
Acc36I ACCTGC 1 cut(s) 788
AccB1I GGYRCC 2 cut(s) 959, 1319
AccBSI CCGCTC 1 cut(s) 507
AccI GTMKAC 2 cut(s) 90, 1329
AciI CCGC 4 cut(s) 260, 436, 507, 957
AclWI GGATC 2 cut(s) 777, 1415
AcsI RAATTY 1 cut(s) 1021
AcuI CTGAAG 3 cut(s) 336, 854, 1385
AdeI CACNNNGTG 1 cut(s) 1282
AfaI GTAC 3 cut(s) 98, 1134, 1491
AflIII ACRYGT 3 cut(s) 93, 237, 675
AgsI TTSAA 4 cut(s) 352, 942, 1073, 1186
AjiI CACGTC 1 cut(s) 94
AjnI CCWGG 4 cut(s) 1016, 1315, 1469, 1528
AjuI GAANNNNNNNTTGG 2 cut(s) 601, 633
Alw21I GWGCWC 2 cut(s) 85, 331
Alw26I GTCTC 4 cut(s) 450, 563, 1018, 1058
AlwI GGATC 2 cut(s) 777, 1415
AlwNI CAGNNNCTG 1 cut(s) 1408
Ama87I CYCGRG 1 cut(s) 1175
AoxI GGCC 1 cut(s) 858
ApeKI GCWGC 1 cut(s) 905
ApoI RAATTY 1 cut(s) 1021
Asp700I GAANNNNTTC 3 cut(s) 134, 839, 916
AspLEI GCGC 4 cut(s) 13, 302, 502, 745
AsuII TTCGAA 1 cut(s) 62
AvaI CYCGRG 1 cut(s) 1175
AxyI CCTNAGG 1 cut(s) 1221
BaeGI GKGCMC 1 cut(s) 1415
BanI GGYRCC 2 cut(s) 959, 1319
BanII GRGCYC 2 cut(s) 331, 1176
Bbv12I GWGCWC 2 cut(s) 85, 331
BbvCI CCTCAGC 1 cut(s) 548
BbvI GCAGC 1 cut(s) 917
BceAI ACGGC 1 cut(s) 115
BcgI CGANNNNNNTGC 2 cut(s) 67, 101
BciT130I CCWGG 4 cut(s) 1018, 1317, 1471, 1530
BcoDI GTCTC 4 cut(s) 450, 563, 1018, 1058
BfaI CTAG 1 cut(s) 606
BfmI CTRYAG 1 cut(s) 1144
BfoI RGCGCY 1 cut(s) 14
BfuAI ACCTGC 1 cut(s) 788
BisI GCNGC 2 cut(s) 260, 906
BlsI GCNGC 2 cut(s) 261, 907
Bme1390I CCNGG 4 cut(s) 1018, 1317, 1471, 1530
BmeT110I CYCGRG 1 cut(s) 1175
BmgBI CACGTC 1 cut(s) 94
BmiI GGNNCC 3 cut(s) 961, 1321, 1468
BmrFI CCNGG 4 cut(s) 1018, 1317, 1471, 1530
BmrI ACTGGG 1 cut(s) 501
BmsI GCATC 1 cut(s) 944
BmuI ACTGGG 1 cut(s) 501
BplI GAGNNNNNCTC 2 cut(s) 205, 237
Bpu10I CCTNAGC 2 cut(s) 330, 548
Bpu14I TTCGAA 1 cut(s) 62
BpuEI CTTGAG 1 cut(s) 545
BsaI GGTCTC 2 cut(s) 563, 1018
BsaJI CCNNGG 2 cut(s) 483, 1470
BsaXI ACNNNNNCTCC 2 cut(s) 318, 348
Bse118I RCCGGY 1 cut(s) 860
Bse1I ACTGG 1 cut(s) 496
Bse21I CCTNAGG 1 cut(s) 1221
Bse3DI GCAATG 2 cut(s) 531, 640
BseBI CCWGG 4 cut(s) 1018, 1317, 1471, 1530
BseDI CCNNGG 2 cut(s) 483, 1470
BseGI GGATG 1 cut(s) 1480
BseMI GCAATG 2 cut(s) 531, 640
BseMII CTCAG 4 cut(s) 203, 344, 539, 1338
BseNI ACTGG 1 cut(s) 496
BseSI GKGCMC 1 cut(s) 1415
BseXI GCAGC 1 cut(s) 917
BshFI GGCC 1 cut(s) 860
BshNI GGYRCC 2 cut(s) 959, 1319
BsiHKAI GWGCWC 2 cut(s) 85, 331
BsiHKCI CYCGRG 1 cut(s) 1175
BsiSI CCGG 2 cut(s) 646, 861
BsmAI GTCTC 4 cut(s) 450, 563, 1018, 1058
BsmI GAATGC 2 cut(s) 127, 790
BsnI GGCC 1 cut(s) 860
Bso31I GGTCTC 2 cut(s) 563, 1018
BsoBI CYCGRG 1 cut(s) 1175
Bsp119I TTCGAA 1 cut(s) 62
Bsp1286I GDGCHC 4 cut(s) 85, 331, 1176, 1415
Bsp143I GATC 3 cut(s) 769, 1249, 1420
Bsp19I CCATGG 1 cut(s) 483
BspACI CCGC 4 cut(s) 260, 436, 507, 957
BspANI GGCC 1 cut(s) 860
BspCNI CTCAG 4 cut(s) 204, 343, 540, 1337
BspLI GGNNCC 3 cut(s) 961, 1321, 1468
BspMI ACCTGC 1 cut(s) 788
BspPI GGATC 2 cut(s) 777, 1415
BspQI GCTCTTC 2 cut(s) 312, 1386
BspT104I TTCGAA 1 cut(s) 62
BspT107I GGYRCC 2 cut(s) 959, 1319
BspTNI GGTCTC 2 cut(s) 563, 1018
BsrBI CCGCTC 1 cut(s) 507
BsrDI GCAATG 2 cut(s) 531, 640
BsrFI RCCGGY 1 cut(s) 860
BsrI ACTGG 1 cut(s) 496
BssAI RCCGGY 1 cut(s) 860
BssECI CCNNGG 2 cut(s) 483, 1470
BssMI GATC 3 cut(s) 769, 1249, 1420
BssNAI GTATAC 1 cut(s) 1330
BssT1I CCWWGG 1 cut(s) 483
Bst1107I GTATAC 1 cut(s) 1330
Bst2UI CCWGG 4 cut(s) 1018, 1317, 1471, 1530
Bst4CI ACNGT 6 cut(s) 306, 513, 722, 1069, 1243, 1410
Bst6I CTCTTC 2 cut(s) 312, 1386
BstAPI GCANNNNNTGC 1 cut(s) 794
BstBI TTCGAA 1 cut(s) 62
BstC8I GCNNGC 4 cut(s) 198, 858, 1304, 1351
BstDEI CTNAG 6 cut(s) 212, 330, 548, 1221, 1324, 1343
BstDSI CCRYGG 1 cut(s) 483
BstEII GGTNACC 1 cut(s) 688
BstF5I GGATG 1 cut(s) 1480
BstH2I RGCGCY 1 cut(s) 14
BstHHI GCGC 4 cut(s) 13, 302, 502, 745
BstKTI GATC 3 cut(s) 772, 1252, 1423
BstMAI GTCTC 4 cut(s) 450, 563, 1018, 1058
BstMBI GATC 3 cut(s) 769, 1249, 1420
BstMWI GCNNNNNNNGC 5 cut(s) 19, 108, 794, 866, 1312
BstNI CCWGG 4 cut(s) 1018, 1317, 1471, 1530
BstNSI RCATGY 2 cut(s) 241, 1306
BstPI GGTNACC 1 cut(s) 688
BstSCI CCNGG 4 cut(s) 1016, 1315, 1469, 1528
BstSFI CTRYAG 1 cut(s) 1144
BstSLI GKGCMC 1 cut(s) 1415
BstV1I GCAGC 1 cut(s) 917
BstXI CCANNNNNNTGG 1 cut(s) 1361
BstZ17I GTATAC 1 cut(s) 1330
Bsu36I CCTNAGG 1 cut(s) 1221
BsuRI GGCC 1 cut(s) 860
BtgI CCRYGG 1 cut(s) 483
BtrI CACGTC 1 cut(s) 94
BtsCI GGATG 1 cut(s) 1480
BtsI GCAGTG 2 cut(s) 85, 826
BtsIMutI CAGTG 4 cut(s) 85, 209, 768, 826
BveI ACCTGC 1 cut(s) 788
Cac8I GCNNGC 4 cut(s) 198, 858, 1304, 1351
CaiI CAGNNNCTG 1 cut(s) 1408
CfoI GCGC 4 cut(s) 13, 302, 502, 745
Cfr10I RCCGGY 1 cut(s) 860
Csp6I GTAC 3 cut(s) 97, 1133, 1490
CviQI GTAC 3 cut(s) 97, 1133, 1490
DdeI CTNAG 6 cut(s) 212, 330, 548, 1221, 1324, 1343
DpnI GATC 3 cut(s) 771, 1251, 1422
DpnII GATC 3 cut(s) 769, 1249, 1420
DraIII CACNNNGTG 1 cut(s) 1282
Eam1104I CTCTTC 2 cut(s) 312, 1386
EarI CTCTTC 2 cut(s) 312, 1386
Ecl136II GAGCTC 1 cut(s) 329
Eco130I CCWWGG 1 cut(s) 483
Eco24I GRGCYC 2 cut(s) 331, 1176
Eco31I GGTCTC 2 cut(s) 563, 1018
Eco53kI GAGCTC 1 cut(s) 329
Eco57I CTGAAG 3 cut(s) 336, 854, 1385
Eco81I CCTNAGG 1 cut(s) 1221
Eco88I CYCGRG 1 cut(s) 1175
Eco91I GGTNACC 1 cut(s) 688
EcoICRI GAGCTC 1 cut(s) 329
EcoO65I GGTNACC 1 cut(s) 688
EcoRI GAATTC 1 cut(s) 1021
EcoRII CCWGG 4 cut(s) 1016, 1315, 1469, 1528
EcoT14I CCWWGG 1 cut(s) 483
EcoT38I GRGCYC 2 cut(s) 331, 1176
ErhI CCWWGG 1 cut(s) 483
FalI AAGNNNNNCTT 2 cut(s) 1326, 1358
FauI CCCGC 1 cut(s) 429
FblI GTMKAC 2 cut(s) 90, 1329
Fnu4HI GCNGC 2 cut(s) 260, 906
FokI GGATG 1 cut(s) 1487
FriOI GRGCYC 2 cut(s) 331, 1176
Fsp4HI GCNGC 2 cut(s) 260, 906
FspBI CTAG 1 cut(s) 606
FspI TGCGCA 2 cut(s) 301, 501
GlaI GCGC 4 cut(s) 12, 301, 501, 744
GluI GCNGC 2 cut(s) 260, 906
HaeII RGCGCY 1 cut(s) 14
HaeIII GGCC 1 cut(s) 860
HapII CCGG 2 cut(s) 646, 861
HhaI GCGC 4 cut(s) 13, 302, 502, 745
Hin6I GCGC 4 cut(s) 11, 300, 500, 743
HinP1I GCGC 4 cut(s) 11, 300, 500, 743
HincII GTYRAC 1 cut(s) 91
HindII GTYRAC 1 cut(s) 91
HindIII AAGCTT 1 cut(s) 1397
HinfI GANTC 3 cut(s) 428, 668, 713
HpaII CCGG 2 cut(s) 646, 861
Hpy166II GTNNAC 4 cut(s) 91, 97, 987, 1330
Hpy188I TCNGA 6 cut(s) 251, 517, 712, 834, 847, 1213
Hpy188III TCNNGA 3 cut(s) 233, 1126, 1277
Hpy8I GTNNAC 4 cut(s) 91, 97, 987, 1330
Hpy99I CGWCG 6 cut(s) 92, 95, 143, 268, 271, 1247
HpyAV CCTTC 7 cut(s) 124, 653, 973, 1054, 1132, 1283, 1300
HpyCH4III ACNGT 6 cut(s) 306, 513, 722, 1069, 1243, 1410
HpyCH4IV ACGT 3 cut(s) 93, 269, 675
HpyCH4V TGCA 9 cut(s) 78, 196, 337, 630, 788, 797, 1043, 1391, 1427
HpyF10VI GCNNNNNNNGC 5 cut(s) 19, 108, 794, 866, 1312
HpyF3I CTNAG 6 cut(s) 212, 330, 548, 1221, 1324, 1343
HpySE526I ACGT 3 cut(s) 93, 269, 675
HspAI GCGC 4 cut(s) 11, 300, 500, 743
Kzo9I GATC 3 cut(s) 769, 1249, 1420
LguI GCTCTTC 2 cut(s) 312, 1386
LmnI GCTCC 4 cut(s) 326, 816, 1228, 1472
Lsp1109I GCAGC 1 cut(s) 917
LweI GCATC 1 cut(s) 944
MaeI CTAG 1 cut(s) 606
MaeII ACGT 3 cut(s) 93, 269, 675
MaeIII GTNAC 5 cut(s) 163, 469, 487, 574, 688
MalI GATC 3 cut(s) 771, 1251, 1422
MbiI CCGCTC 1 cut(s) 507
MboI GATC 3 cut(s) 769, 1249, 1420
MboII GAAGA 5 cut(s) 329, 836, 963, 1104, 1373
MhlI GDGCHC 4 cut(s) 85, 331, 1176, 1415
MluCI AATT 4 cut(s) 568, 792, 1021, 1073
MlyI GAGTC 2 cut(s) 437, 722
MmeI TCCRAC 4 cut(s) 229, 696, 1143, 1520
MnlI CCTC 9 cut(s) 159, 514, 543, 724, 846, 1093, 1333, 1457, 1505
MroXI GAANNNNTTC 3 cut(s) 134, 839, 916
MseI TTAA 5 cut(s) 242, 444, 899, 927, 1296
MslI CAYNNNNRTG 1 cut(s) 156
MspI CCGG 2 cut(s) 646, 861
MspR9I CCNGG 4 cut(s) 1018, 1317, 1471, 1530
Mva1269I GAATGC 2 cut(s) 127, 790
MvaI CCWGG 4 cut(s) 1018, 1317, 1471, 1530
MwoI GCNNNNNNNGC 5 cut(s) 19, 108, 794, 866, 1312
NcoI CCATGG 1 cut(s) 483
NdeII GATC 3 cut(s) 769, 1249, 1420
NlaIV GGNNCC 3 cut(s) 961, 1321, 1468
NmuCI GTSAC 3 cut(s) 163, 469, 574
NsbI TGCGCA 2 cut(s) 301, 501
NspI RCATGY 2 cut(s) 241, 1306
NspV TTCGAA 1 cut(s) 62
PaeI GCATGC 1 cut(s) 1306
PaeR7I CTCGAG 1 cut(s) 1175
PciI ACATGT 1 cut(s) 237
PciSI GCTCTTC 2 cut(s) 312, 1386
PcsI WCGNNNNNNNCGW 1 cut(s) 1242
PctI GAATGC 2 cut(s) 127, 790
PdmI GAANNNNTTC 3 cut(s) 134, 839, 916
PfeI GAWTC 1 cut(s) 668
PfoI TCCNGGA 1 cut(s) 1016
PkrI GCNGC 2 cut(s) 261, 907
PleI GAGTC 2 cut(s) 436, 721
PpsI GAGTC 2 cut(s) 436, 721
PscI ACATGT 1 cut(s) 237
Psp124BI GAGCTC 1 cut(s) 331
Psp6I CCWGG 4 cut(s) 1016, 1315, 1469, 1528
PspEI GGTNACC 1 cut(s) 688
PspGI CCWGG 4 cut(s) 1016, 1315, 1469, 1528
PspN4I GGNNCC 3 cut(s) 961, 1321, 1468
PspXI VCTCGAGB 1 cut(s) 1175
PstNI CAGNNNCTG 1 cut(s) 1408
RsaI GTAC 3 cut(s) 98, 1134, 1491
RsaNI GTAC 3 cut(s) 97, 1133, 1490
RseI CAYNNNNRTG 1 cut(s) 156
SacI GAGCTC 1 cut(s) 331
SalI GTCGAC 1 cut(s) 89
SapI GCTCTTC 2 cut(s) 312, 1386
SaqAI TTAA 5 cut(s) 242, 444, 899, 927, 1296
SatI GCNGC 2 cut(s) 260, 906
Sau3AI GATC 3 cut(s) 769, 1249, 1420
SchI GAGTC 2 cut(s) 437, 722
ScrFI CCNGG 4 cut(s) 1018, 1317, 1471, 1530
SduI GDGCHC 4 cut(s) 85, 331, 1176, 1415
SfaNI GCATC 1 cut(s) 944
SfcI CTRYAG 1 cut(s) 1144
Sfr274I CTCGAG 1 cut(s) 1175
SfuI TTCGAA 1 cut(s) 62
SgrDI CGTCGACG 1 cut(s) 89
SlaI CTCGAG 1 cut(s) 1175
SmiMI CAYNNNNRTG 1 cut(s) 156
SmlI CTYRAG 2 cut(s) 560, 1175
SmoI CTYRAG 2 cut(s) 560, 1175
SphI GCATGC 1 cut(s) 1306
Sse9I AATT 4 cut(s) 568, 792, 1021, 1073
SsiI CCGC 4 cut(s) 260, 436, 507, 957
SspI AATATT 1 cut(s) 811
SspMI CTAG 1 cut(s) 606
SstI GAGCTC 1 cut(s) 331
StyD4I CCNGG 4 cut(s) 1016, 1315, 1469, 1528
StyI CCWWGG 1 cut(s) 483
TaaI ACNGT 6 cut(s) 306, 513, 722, 1069, 1243, 1410
TaiI ACGT 3 cut(s) 96, 272, 678
TaqI TCGA 4 cut(s) 62, 90, 138, 1176
TasI AATT 4 cut(s) 568, 792, 1021, 1073
TatI WGTACW 1 cut(s) 1132
TauI GCSGC 1 cut(s) 262
TfiI GAWTC 1 cut(s) 668
Tru1I TTAA 5 cut(s) 242, 444, 899, 927, 1296
Tru9I TTAA 5 cut(s) 242, 444, 899, 927, 1296
TscAI CASTG 4 cut(s) 85, 216, 768, 826
TseFI GTSAC 3 cut(s) 163, 469, 574
TseI GCWGC 1 cut(s) 905
Tsp45I GTSAC 3 cut(s) 163, 469, 574
TspDTI ATGAA 4 cut(s) 588, 1010, 1014, 1349
TspGWI ACGGA 2 cut(s) 728, 1507
TspRI CASTG 4 cut(s) 85, 216, 768, 826
XapI RAATTY 1 cut(s) 1021
XceI RCATGY 2 cut(s) 241, 1306
XhoI CTCGAG 1 cut(s) 1175
XmiI GTMKAC 2 cut(s) 90, 1329
XmnI GAANNNNTTC 3 cut(s) 134, 839, 916
XspI CTAG 1 cut(s) 606
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.