pycom11g08090

Pentatricopeptide repeat-containing protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr11
OG0008107
Physical Location & Seq
Reverse (-)
6208124 .. 6209854
1731 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom11g08090.1

Sequence Viewer

Length: 1731 bp
ATGACAGTGACCCAGAAGCTTGCAAAGCCATTTGGTTTGGAGCTGCTGGCTCAGTTGCAACAGGGTGGGTCTTCACTCTCCCCTCAAACCCTTAACAAAATCGTATCTTCTTGCGCAAAGTCGGCTTCTTTGGATTCGGGCATTAAGATTCACGCATTTGCTATCAAGTTGGGTTTTTGCTCAAATGTCTATATATCCAGTGCTCTCGTTGATTTGTATGGCAAATGCGGTTCGTTGCGGAATGCTCAGATACTGTTTGATGAAATGCCCGAGAGAAATGTTGTGACTTGGAATTCCCTGATTTCTGGTTATTTGCAAGCTGAGTTGCCAAAAAGGGCAGTTGGGTTGTTTCTGGAGATGTTGAAGGTGGGGATACTGCCAACGCCGTTCAGTCTTTCGGGCGTCTTGGTGGGTTGCTCACAGCTAGAAGCTGAAGAGCTTGGGGCTCAGGTGCATGGTTTGAGCATGAAAATGGGGATTTGCTGCAATGTTGTTTTGGGAACTGGATTGATAGATATGTATTCGAAGTGTTGTAGTGTTAACGAATCAAAGCGTGTGTTTAATGAAATGCCAGATAAGAATGTTATAACTTGGACTTCCATGGTTACTGGCTATGCGCAGAATGGACAATCCAACAAGGCGATGATTTTAGCGAGGGAAATGTTGCGTTTGGGTCACAAACCAAATTGTGTAACTTATAATAGCTTGTTGAGTTCATTTTCGAGCCCGGACTTTTGGAATCATTGCAGGCAAATCCATTGCCGGATAATGAAAGAAGGTTTTGAGTTTAATGCATATATTTTGGTTACACTTATGACGGTATATTCAGAATGTAGCAATAGCTTGGAAGACTTTCAAAAGCTTTGCTCGTGCGTTGCAGTATGGGATCAAATCTCATGGAATGCAGTAATCGCTGGTTTTTCTAATATTGGAAATAGTGAGGAAGCTCTGCAGTGTTTCTCTGAAATGAGGCAGGCTGGAGTTTCTATGAACTTCTTCACATTTGCAAGCATTTTAAGCGCGGTAGGAAGTCTTTCAGCCCTTGAAGAGGGACGGAAAATCCATGCTCTCGTTTTCAAGAGTGGGCATGCTTCAAATTTATGTGTTCAGAATGGGCTTGTTTCCATGTATGCAAGATGCGGTGCCATCCATGATTCAAAGCGAGTGTTTAATTTGATGAAAGAACATGATGACGTATCCTGGAATTCATTATTATCAGGTTATGCTCATCATGGGTTTGGGTTGGAGGCTGTTGAACTGTTTGAGGAAATGAGAAGGACAGAGGTCAAACCGGATAGTACCACCTTCCTCATTGTACTAACTGCTTGTAGTCATGTTGGTTTAGTGGACAAAGGATTTGAGTATTTCAATCTGATGAGAAATGATGATTTTTGTGCACCCCCGAAAATGGAGCATTATGCAACAGTTGTTGACCTTTTTGGTCGAGCTGGAAATCTTCATGAAGCACAAGCCTTTGTTGATAACATGGCAATCAAGGCGGGACCATCAGTGTACAAAGCCCTGCTCAGTGCTTGCCAAGTTCATGGAAATAAGGAAATCGCTCTTCGTTCTGCACACAAGCTTCAGGAACTGTCCCCAAATGATCCTGCAACTTACATACTCCTGTCGAATGTGTTGCTGACGAGAGGCTACTGGGATGATGCAGCAGGAGTAAGGAAGCTCATGTATGATAGAGGAGTAAGAAAAACTCCCGGTTACAGTTGGATTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
PPR PPR_2 Pkinase_Tyr

Protein Analysis

577

Amino Acids

63.51

Weight (kDa)

6.95

Isoelectric Point (pI)

35.84

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PPR_2 PF13041 92 - 140 3.5e-09 PPR repeat family
PPR PF01535 95 - 125 3.7e-07 PPR repeat
PPR_2 PF13041 193 - 240 2.4e-12 PPR repeat family
PPR_3 PF13812 194 - 240 3.8e-06 Pentatricopeptide repeat domain
PPR_2 PF13041 296 - 341 1.6e-09 PPR repeat family
PPR PF01535 298 - 328 1.6e-06 PPR repeat
PPR_2 PF13041 397 - 444 1.3e-11 PPR repeat family
PPR PF01535 399 - 426 2.7e-08 PPR repeat
E_motif PF20431 515 - 576 4.4e-15 E motif
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 2 cut(s) 587, 699
Acc16I TGCGCA 2 cut(s) 115, 618
AccB1I GGYRCC 1 cut(s) 1142
AccII CGCG 1 cut(s) 1022
AciI CCGC 5 cut(s) 228, 238, 1022, 1140, 1499
AclWI GGATC 2 cut(s) 894, 1598
AcsI RAATTY 3 cut(s) 292, 1096, 1204
AcuI CTGAAG 2 cut(s) 453, 1568
AcyI GRCGYC 1 cut(s) 402
AfaI GTAC 3 cut(s) 1300, 1317, 1514
AfiI CCNNNNNNNGG 2 cut(s) 1048, 1408
AgsI TTSAA 8 cut(s) 364, 857, 1046, 1078, 1095, 1158, 1256, 1369
AjnI CCWGG 1 cut(s) 1199
Alw21I GWGCWC 2 cut(s) 205, 1399
Alw44I GTGCAC 1 cut(s) 1395
AlwI GGATC 2 cut(s) 894, 1598
AlwNI CAGNNNCTG 2 cut(s) 253, 1591
Ama87I CYCGRG 1 cut(s) 269
ApaLI GTGCAC 1 cut(s) 1395
ApeKI GCWGC 3 cut(s) 43, 483, 1664
ApoI RAATTY 3 cut(s) 292, 1096, 1204
ArsI GACNNNNNNTTYG 2 cut(s) 1340, 1372
Asp700I GAANNNNTTC 3 cut(s) 852, 995, 1033
AspLEI GCGC 3 cut(s) 116, 619, 1022
AspS9I GGNCC 1 cut(s) 1502
AsuC2I CCSGG 2 cut(s) 728, 1713
AsuII TTCGAA 1 cut(s) 524
AvaI CYCGRG 1 cut(s) 269
AvaII GGWCC 1 cut(s) 1502
BaeGI GKGCMC 1 cut(s) 1399
BanI GGYRCC 1 cut(s) 1142
BanII GRGCYC 2 cut(s) 448, 728
BauI CACGAG 1 cut(s) 868
BbsI GAAGAC 2 cut(s) 63, 855
Bbv12I GWGCWC 2 cut(s) 205, 1399
BbvI GCAGC 3 cut(s) 30, 470, 1676
BccI CCATC 2 cut(s) 1154, 1513
BceAI ACGGC 1 cut(s) 370
BcgI CGANNNNNNTGC 2 cut(s) 1618, 1652
BciT130I CCWGG 1 cut(s) 1201
BciVI GTATCC 2 cut(s) 366, 1207
BcnI CCSGG 2 cut(s) 728, 1713
BfaI CTAG 1 cut(s) 425
BfmI CTRYAG 1 cut(s) 950
BfuI GTATCC 2 cut(s) 366, 1207
BisI GCNGC 3 cut(s) 44, 484, 1665
BlsI GCNGC 3 cut(s) 45, 485, 1666
Bme1390I CCNGG 3 cut(s) 728, 1201, 1713
Bme18I GGWCC 1 cut(s) 1502
BmeT110I CYCGRG 1 cut(s) 269
BmgT120I GGNCC 1 cut(s) 1502
BmiI GGNNCC 2 cut(s) 1144, 1503
BmrFI CCNGG 3 cut(s) 728, 1201, 1713
BmrI ACTGGG 1 cut(s) 1663
BmsI GCATC 2 cut(s) 1127, 1651
BmuI ACTGGG 1 cut(s) 1663
BoxI GACNNNNGTC 1 cut(s) 1283
BpiI GAAGAC 2 cut(s) 63, 855
BpmI CTGGAG 2 cut(s) 374, 999
Bpu10I CCTNAGC 1 cut(s) 447
Bpu14I TTCGAA 1 cut(s) 524
BpuMI CCSGG 2 cut(s) 728, 1713
BsaHI GRCGYC 1 cut(s) 402
BsaJI CCNNGG 1 cut(s) 600
BsaWI WCCGGW 1 cut(s) 1291
Bsc4I CCNNNNNNNGG 2 cut(s) 1048, 1408
Bse1I ACTGG 4 cut(s) 198, 508, 613, 1658
Bse3DI GCAATG 3 cut(s) 493, 742, 757
BseBI CCWGG 1 cut(s) 1201
BseDI CCNNGG 1 cut(s) 600
BseGI GGATG 2 cut(s) 1146, 1663
BseLI CCNNNNNNNGG 2 cut(s) 1048, 1408
BseMI GCAATG 3 cut(s) 493, 742, 757
BseMII CTCAG 5 cut(s) 65, 260, 312, 461, 1540
BseNI ACTGG 4 cut(s) 198, 508, 613, 1658
BseRI GAGGAG 1 cut(s) 1710
BseSI GKGCMC 1 cut(s) 1399
BseXI GCAGC 3 cut(s) 30, 470, 1676
BsgI GTGCAG 1 cut(s) 1557
Bsh1236I CGCG 1 cut(s) 1022
BshNI GGYRCC 1 cut(s) 1142
BsiHKAI GWGCWC 2 cut(s) 205, 1399
BsiHKCI CYCGRG 1 cut(s) 269
BsiSI CCGG 4 cut(s) 728, 763, 1292, 1713
BslFI GGGAC 3 cut(s) 1065, 1515, 1579
BslI CCNNNNNNNGG 2 cut(s) 1048, 1408
BsmFI GGGAC 3 cut(s) 1065, 1515, 1579
BsmI GAATGC 2 cut(s) 247, 907
BsoBI CYCGRG 1 cut(s) 269
Bsp119I TTCGAA 1 cut(s) 524
Bsp1286I GDGCHC 4 cut(s) 205, 448, 728, 1399
Bsp1407I TGTACA 1 cut(s) 1512
Bsp143I GATC 2 cut(s) 886, 1603
Bsp19I CCATGG 1 cut(s) 600
BspACI CCGC 5 cut(s) 228, 238, 1022, 1140, 1499
BspCNI CTCAG 5 cut(s) 64, 259, 313, 460, 1539
BspFNI CGCG 1 cut(s) 1022
BspHI TCATGA 1 cut(s) 1459
BspLI GGNNCC 2 cut(s) 1144, 1503
BspMAI CTGCAG 1 cut(s) 954
BspPI GGATC 2 cut(s) 894, 1598
BspQI GCTCTTC 2 cut(s) 429, 1569
BspT104I TTCGAA 1 cut(s) 524
BspT107I GGYRCC 1 cut(s) 1142
BsrDI GCAATG 3 cut(s) 493, 742, 757
BsrGI TGTACA 1 cut(s) 1512
BsrI ACTGG 4 cut(s) 198, 508, 613, 1658
BssECI CCNNGG 1 cut(s) 600
BssMI GATC 2 cut(s) 886, 1603
BssNI GRCGYC 1 cut(s) 402
BssSI CACGAG 1 cut(s) 868
BssT1I CCWWGG 1 cut(s) 600
Bst2BI CACGAG 1 cut(s) 868
Bst2UI CCWGG 1 cut(s) 1201
Bst4CI ACNGT 7 cut(s) 7, 255, 820, 1260, 1426, 1593, 1721
Bst6I CTCTTC 3 cut(s) 429, 1041, 1569
BstACI GRCGYC 1 cut(s) 402
BstAUI TGTACA 1 cut(s) 1512
BstBI TTCGAA 1 cut(s) 524
BstC8I GCNNGC 8 cut(s) 21, 48, 318, 749, 975, 1009, 1089, 1534
BstDEI CTNAG 5 cut(s) 51, 246, 321, 447, 1526
BstDSI CCRYGG 1 cut(s) 600
BstENI CCTNNNNNAGG 1 cut(s) 1046
BstF5I GGATG 2 cut(s) 1146, 1663
BstFNI CGCG 1 cut(s) 1022
BstHHI GCGC 3 cut(s) 116, 619, 1022
BstKTI GATC 2 cut(s) 889, 1606
BstMBI GATC 2 cut(s) 886, 1603
BstMWI GCNNNNNNNGC 5 cut(s) 25, 122, 911, 1017, 1496
BstNI CCWGG 1 cut(s) 1201
BstNSI RCATGY 1 cut(s) 1091
BstPAI GACNNNNGTC 1 cut(s) 1283
BstSCI CCNGG 3 cut(s) 726, 1199, 1711
BstSFI CTRYAG 1 cut(s) 950
BstSLI GKGCMC 1 cut(s) 1399
BstUI CGCG 1 cut(s) 1022
BstV1I GCAGC 3 cut(s) 30, 470, 1676
BstV2I GAAGAC 2 cut(s) 63, 855
BstXI CCANNNNNNTGG 1 cut(s) 1544
BsuI GTATCC 2 cut(s) 366, 1207
BtgI CCRYGG 1 cut(s) 600
BtgZI GCGATG 1 cut(s) 656
BtsCI GGATG 2 cut(s) 1146, 1663
BtsI GCAGTG 1 cut(s) 959
BtsIMutI CAGTG 5 cut(s) 12, 205, 959, 1515, 1534
Cac8I GCNNGC 8 cut(s) 21, 48, 318, 749, 975, 1009, 1089, 1534
CaiI CAGNNNCTG 2 cut(s) 253, 1591
CciI TCATGA 1 cut(s) 1459
CfoI GCGC 3 cut(s) 116, 619, 1022
Cfr13I GGNCC 1 cut(s) 1502
CseI GACGC 1 cut(s) 391
Csp6I GTAC 3 cut(s) 1299, 1316, 1513
CviQI GTAC 3 cut(s) 1299, 1316, 1513
DdeI CTNAG 5 cut(s) 51, 246, 321, 447, 1526
DpnI GATC 2 cut(s) 888, 1605
DpnII GATC 2 cut(s) 886, 1603
Eam1104I CTCTTC 3 cut(s) 429, 1041, 1569
EarI CTCTTC 3 cut(s) 429, 1041, 1569
Eco130I CCWWGG 1 cut(s) 600
Eco24I GRGCYC 2 cut(s) 448, 728
Eco47I GGWCC 1 cut(s) 1502
Eco57I CTGAAG 2 cut(s) 453, 1568
Eco88I CYCGRG 1 cut(s) 269
EcoNI CCTNNNNNAGG 1 cut(s) 1046
EcoRI GAATTC 2 cut(s) 292, 1204
EcoRII CCWGG 1 cut(s) 1199
EcoT14I CCWWGG 1 cut(s) 600
EcoT22I ATGCAT 1 cut(s) 796
EcoT38I GRGCYC 2 cut(s) 448, 728
ErhI CCWWGG 1 cut(s) 600
FaqI GGGAC 3 cut(s) 1065, 1515, 1579
FauI CCCGC 1 cut(s) 1492
Fnu4HI GCNGC 3 cut(s) 44, 484, 1665
FokI GGATG 2 cut(s) 1133, 1670
FriOI GRGCYC 2 cut(s) 448, 728
Fsp4HI GCNGC 3 cut(s) 44, 484, 1665
FspBI CTAG 1 cut(s) 425
FspI TGCGCA 2 cut(s) 115, 618
GlaI GCGC 3 cut(s) 115, 618, 1021
GluI GCNGC 3 cut(s) 44, 484, 1665
GsuI CTGGAG 2 cut(s) 374, 999
HapII CCGG 4 cut(s) 728, 763, 1292, 1713
HgaI GACGC 1 cut(s) 391
HhaI GCGC 3 cut(s) 116, 619, 1022
Hin1I GRCGYC 1 cut(s) 402
Hin6I GCGC 3 cut(s) 114, 617, 1020
HinP1I GCGC 3 cut(s) 114, 617, 1020
HincII GTYRAC 2 cut(s) 541, 1432
HindII GTYRAC 2 cut(s) 541, 1432
HindIII AAGCTT 3 cut(s) 17, 860, 1580
HinfI GANTC 5 cut(s) 134, 148, 545, 739, 1154
HpaI GTTAAC 1 cut(s) 541
HpaII CCGG 4 cut(s) 728, 763, 1292, 1713
Hpy166II GTNNAC 5 cut(s) 541, 1348, 1397, 1432, 1513
Hpy188I TCNGA 5 cut(s) 249, 829, 964, 1110, 1374
Hpy188III TCNNGA 4 cut(s) 353, 1078, 1460, 1586
Hpy8I GTNNAC 5 cut(s) 541, 1348, 1397, 1432, 1513
HpyAV CCTTC 4 cut(s) 358, 770, 1269, 1315
HpyCH4III ACNGT 7 cut(s) 7, 255, 820, 1260, 1426, 1593, 1721
HpyCH4IV ACGT 1 cut(s) 1194
HpyF10VI GCNNNNNNNGC 5 cut(s) 25, 122, 911, 1017, 1496
HpyF3I CTNAG 5 cut(s) 51, 246, 321, 447, 1526
HpySE526I ACGT 1 cut(s) 1194
Hsp92I GRCGYC 1 cut(s) 402
HspAI GCGC 3 cut(s) 114, 617, 1020
KspAI GTTAAC 1 cut(s) 541
Kzo9I GATC 2 cut(s) 886, 1603
LguI GCTCTTC 2 cut(s) 429, 1569
LmnI GCTCC 2 cut(s) 40, 1411
Lsp1109I GCAGC 3 cut(s) 30, 470, 1676
LweI GCATC 2 cut(s) 1127, 1651
MaeI CTAG 1 cut(s) 425
MaeII ACGT 1 cut(s) 1194
MaeIII GTNAC 7 cut(s) 7, 283, 604, 674, 691, 805, 1715
MalI GATC 2 cut(s) 888, 1605
MboI GATC 2 cut(s) 886, 1603
MboII GAAGA 8 cut(s) 63, 99, 446, 860, 988, 1058, 1448, 1556
MhlI GDGCHC 4 cut(s) 205, 448, 728, 1399
MluCI AATT 5 cut(s) 292, 685, 1096, 1171, 1204
MmeI TCCRAC 3 cut(s) 657, 1224, 1703
Mph1103I ATGCAT 1 cut(s) 796
MroXI GAANNNNTTC 3 cut(s) 852, 995, 1033
MseI TTAA 7 cut(s) 93, 144, 540, 561, 789, 1016, 1170
MslI CAYNNNNRTG 1 cut(s) 470
MspI CCGG 4 cut(s) 728, 763, 1292, 1713
MspR9I CCNGG 3 cut(s) 728, 1201, 1713
Mva1269I GAATGC 2 cut(s) 247, 907
MvaI CCWGG 1 cut(s) 1201
MvnI CGCG 1 cut(s) 1022
MwoI GCNNNNNNNGC 5 cut(s) 25, 122, 911, 1017, 1496
NciI CCSGG 2 cut(s) 728, 1713
NcoI CCATGG 1 cut(s) 600
NdeII GATC 2 cut(s) 886, 1603
NlaIV GGNNCC 2 cut(s) 1144, 1503
NmuCI GTSAC 3 cut(s) 7, 283, 674
NsbI TGCGCA 2 cut(s) 115, 618
NsiI ATGCAT 1 cut(s) 796
NspI RCATGY 1 cut(s) 1091
NspV TTCGAA 1 cut(s) 524
PaeI GCATGC 1 cut(s) 1091
PagI TCATGA 1 cut(s) 1459
PciSI GCTCTTC 2 cut(s) 429, 1569
PctI GAATGC 2 cut(s) 247, 907
PdmI GAANNNNTTC 3 cut(s) 852, 995, 1033
PfeI GAWTC 5 cut(s) 134, 148, 545, 739, 1154
PfoI TCCNGGA 1 cut(s) 1199
PkrI GCNGC 3 cut(s) 45, 485, 1666
PshAI GACNNNNGTC 1 cut(s) 1283
PsiI TTATAA 2 cut(s) 587, 699
Psp6I CCWGG 1 cut(s) 1199
PspGI CCWGG 1 cut(s) 1199
PspN4I GGNNCC 2 cut(s) 1144, 1503
PspPI GGNCC 1 cut(s) 1502
PstI CTGCAG 1 cut(s) 954
PstNI CAGNNNCTG 2 cut(s) 253, 1591
RsaI GTAC 3 cut(s) 1300, 1317, 1514
RsaNI GTAC 3 cut(s) 1299, 1316, 1513
RseI CAYNNNNRTG 1 cut(s) 470
SapI GCTCTTC 2 cut(s) 429, 1569
SaqAI TTAA 7 cut(s) 93, 144, 540, 561, 789, 1016, 1170
SatI GCNGC 3 cut(s) 44, 484, 1665
Sau3AI GATC 2 cut(s) 886, 1603
Sau96I GGNCC 1 cut(s) 1502
ScrFI CCNGG 3 cut(s) 728, 1201, 1713
SduI GDGCHC 4 cut(s) 205, 448, 728, 1399
SfaNI GCATC 2 cut(s) 1127, 1651
SfcI CTRYAG 1 cut(s) 950
SfuI TTCGAA 1 cut(s) 524
SinI GGWCC 1 cut(s) 1502
SmiMI CAYNNNNRTG 1 cut(s) 470
SphI GCATGC 1 cut(s) 1091
Sse9I AATT 5 cut(s) 292, 685, 1096, 1171, 1204
SsiI CCGC 5 cut(s) 228, 238, 1022, 1140, 1499
SspI AATATT 1 cut(s) 928
SspMI CTAG 1 cut(s) 425
StyD4I CCNGG 3 cut(s) 726, 1199, 1711
StyI CCWWGG 1 cut(s) 600
TaaI ACNGT 7 cut(s) 7, 255, 820, 1260, 1426, 1593, 1721
TaiI ACGT 1 cut(s) 1197
TaqI TCGA 4 cut(s) 524, 722, 1444, 1628
TasI AATT 5 cut(s) 292, 685, 1096, 1171, 1204
TatI WGTACW 2 cut(s) 1315, 1512
TfiI GAWTC 5 cut(s) 134, 148, 545, 739, 1154
Tru1I TTAA 7 cut(s) 93, 144, 540, 561, 789, 1016, 1170
Tru9I TTAA 7 cut(s) 93, 144, 540, 561, 789, 1016, 1170
TscAI CASTG 5 cut(s) 12, 205, 959, 1515, 1534
TseFI GTSAC 3 cut(s) 7, 283, 674
TseI GCWGC 3 cut(s) 43, 483, 1664
Tsp45I GTSAC 3 cut(s) 7, 283, 674
TspGWI ACGGA 1 cut(s) 1069
TspRI CASTG 5 cut(s) 12, 205, 959, 1515, 1534
VneI GTGCAC 1 cut(s) 1395
VpaK11BI GGWCC 1 cut(s) 1502
XagI CCTNNNNNAGG 1 cut(s) 1046
XapI RAATTY 3 cut(s) 292, 1096, 1204
XceI RCATGY 1 cut(s) 1091
XmnI GAANNNNTTC 3 cut(s) 852, 995, 1033
XspI CTAG 1 cut(s) 425
Zsp2I ATGCAT 1 cut(s) 796
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.