Rroxscaffold_5G00352300

Pentatricopeptide repeat-containing protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000005
OG0008107
Physical Location & Seq
Forward (+)
29300510 .. 29307400
6891 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_5G00352300.1

Sequence Viewer

Length: 609 bp
ATGTGCGCGAGTGGGCGATCCGATGAGGCAATGACTTTAGTGAGAGAAATGTCGCTCGGGCAAAGTTTTCGTGCGAAGGTCAAGATAGACCTCTTTCCTTCCCGGTTCGGCAGTCCCGGCCCGGCGTCCGGTGCAGCAGTCCGGTTCGGCTTCCGGTTCAGCGAGTCCGGTCCCGGCGTCCGGTGCAGCAGTTCGGTTCGGCGTCCGAGTTCAGCGGTTCAGTTCCGTAATACCAGTTCGAGCCCCTCCCCGCCTCTCTCACTCCCGACCAGGCCGGAAAGCAAAGCTTCTCCGAGTGGTTACCGGATGTGCGCAGAGTGGGCGATCGATGAGGCAATGACTTTAGTGAGAGAAATGCCGCCGTTGGGTCTCAAGCCGAATTATGCTACTTATAATAGTTTGTTGAGTTCATTTACTAGCATGGACTTTTGGGCTTGTTGCAGGCAAATCCATTGCCGGATAGTGAAAGAAGGGTTTGATTCCAATGTGTATATTGTGGTTACCCTTTTGACCTTATATTCGCAGTCCGATAGTAGCTTGAAGGACTTCGTGCGCTTTGCTCATGTGTTTCGGTGTGGGATCAAATCTCATGGAATAGCGAAGAGGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
PPR PPR_2 Pkinase_Tyr

Protein Analysis

202

Amino Acids

22.24

Weight (kDa)

9.67

Isoelectric Point (pI)

52.9

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 393
Acc16I TGCGCA 1 cut(s) 313
AccII CGCG 1 cut(s) 8
AciI CCGC 3 cut(s) 215, 251, 359
AclWI GGATC 2 cut(s) 12, 587
AcyI GRCGYC 3 cut(s) 125, 177, 202
AfiI CCNNNNNNNGG 3 cut(s) 128, 180, 365
AgsI TTSAA 1 cut(s) 541
AjnI CCWGG 1 cut(s) 269
AluBI AGCT 2 cut(s) 287, 537
AluI AGCT 2 cut(s) 287, 537
Alw26I GTCTC 1 cut(s) 374
AlwI GGATC 2 cut(s) 12, 587
Ama87I CYCGRG 1 cut(s) 56
AoxI GGCC 2 cut(s) 118, 272
ApeKI GCWGC 2 cut(s) 134, 186
ArsI GACNNNNNNTTYG 2 cut(s) 502, 534
Asp700I GAANNNNTTC 1 cut(s) 545
AspLEI GCGC 3 cut(s) 8, 314, 555
AspS9I GGNCC 2 cut(s) 119, 170
AsuC2I CCSGG 4 cut(s) 103, 117, 122, 174
AvaI CYCGRG 1 cut(s) 56
AvaII GGWCC 1 cut(s) 170
BanII GRGCYC 1 cut(s) 245
BbvI GCAGC 2 cut(s) 146, 198
BceAI ACGGC 1 cut(s) 346
BcgI CGANNNNNNTGC 2 cut(s) 50, 84
BciT130I CCWGG 1 cut(s) 271
BcnI CCSGG 4 cut(s) 103, 117, 122, 174
BcoDI GTCTC 1 cut(s) 374
BfaI CTAG 1 cut(s) 417
BisI GCNGC 3 cut(s) 135, 187, 359
BlsI GCNGC 3 cut(s) 136, 188, 360
Bme1390I CCNGG 5 cut(s) 103, 117, 122, 174, 271
Bme18I GGWCC 1 cut(s) 170
BmeT110I CYCGRG 1 cut(s) 56
BmgT120I GGNCC 2 cut(s) 119, 170
BmiI GGNNCC 1 cut(s) 172
BmrFI CCNGG 5 cut(s) 103, 117, 122, 174, 271
BpuEI CTTGAG 1 cut(s) 356
BpuMI CCSGG 4 cut(s) 103, 117, 122, 174
Bsa29I ATCGAT 1 cut(s) 327
BsaHI GRCGYC 3 cut(s) 125, 177, 202
BsaI GGTCTC 1 cut(s) 374
BsaWI WCCGGW 6 cut(s) 128, 141, 153, 167, 180, 303
Bsc4I CCNNNNNNNGG 3 cut(s) 128, 180, 365
Bse1I ACTGG 1 cut(s) 234
Bse3DI GCAATG 3 cut(s) 36, 342, 451
BseBI CCWGG 1 cut(s) 271
BseCI ATCGAT 1 cut(s) 327
BseGI GGATG 1 cut(s) 312
BseLI CCNNNNNNNGG 3 cut(s) 128, 180, 365
BseMI GCAATG 3 cut(s) 36, 342, 451
BseNI ACTGG 1 cut(s) 234
BseXI GCAGC 2 cut(s) 146, 198
BsgI GTGCAG 2 cut(s) 153, 205
Bsh1236I CGCG 1 cut(s) 8
Bsh1285I CGRYCG 1 cut(s) 327
BshFI GGCC 2 cut(s) 120, 274
BshVI ATCGAT 1 cut(s) 327
BsiEI CGRYCG 1 cut(s) 327
BsiHKCI CYCGRG 1 cut(s) 56
BslFI GGGAC 2 cut(s) 99, 156
BslI CCNNNNNNNGG 3 cut(s) 128, 180, 365
BsmAI GTCTC 1 cut(s) 374
BsmFI GGGAC 2 cut(s) 99, 156
BsnI GGCC 2 cut(s) 120, 274
Bso31I GGTCTC 1 cut(s) 374
BsoBI CYCGRG 1 cut(s) 56
Bsp1286I GDGCHC 1 cut(s) 245
Bsp143I GATC 3 cut(s) 17, 324, 579
BspACI CCGC 3 cut(s) 215, 251, 359
BspANI GGCC 2 cut(s) 120, 274
BspDI ATCGAT 1 cut(s) 327
BspFNI CGCG 1 cut(s) 8
BspLI GGNNCC 1 cut(s) 172
BspPI GGATC 2 cut(s) 12, 587
BspTNI GGTCTC 1 cut(s) 374
BsrDI GCAATG 3 cut(s) 36, 342, 451
BsrI ACTGG 1 cut(s) 234
BssMI GATC 3 cut(s) 17, 324, 579
BssNI GRCGYC 3 cut(s) 125, 177, 202
Bst2UI CCWGG 1 cut(s) 271
Bst6I CTCTTC 1 cut(s) 596
BstACI GRCGYC 3 cut(s) 125, 177, 202
BstC8I GCNNGC 1 cut(s) 443
BstEII GGTNACC 2 cut(s) 299, 499
BstF5I GGATG 1 cut(s) 312
BstFNI CGCG 1 cut(s) 8
BstHHI GCGC 3 cut(s) 8, 314, 555
BstKTI GATC 3 cut(s) 20, 327, 582
BstMAI GTCTC 1 cut(s) 374
BstMBI GATC 3 cut(s) 17, 324, 579
BstMCI CGRYCG 1 cut(s) 327
BstMWI GCNNNNNNNGC 4 cut(s) 117, 131, 183, 320
BstNI CCWGG 1 cut(s) 271
BstPI GGTNACC 2 cut(s) 299, 499
BstSCI CCNGG 5 cut(s) 101, 115, 120, 172, 269
BstUI CGCG 1 cut(s) 8
BstV1I GCAGC 2 cut(s) 146, 198
Bsu15I ATCGAT 1 cut(s) 327
BsuRI GGCC 2 cut(s) 120, 274
BsuTUI ATCGAT 1 cut(s) 327
BtsCI GGATG 1 cut(s) 312
Cac8I GCNNGC 1 cut(s) 443
CfoI GCGC 3 cut(s) 8, 314, 555
Cfr13I GGNCC 2 cut(s) 119, 170
ClaI ATCGAT 1 cut(s) 327
CseI GACGC 3 cut(s) 114, 166, 191
CviAII CATG 3 cut(s) 421, 563, 590
CviJI RGCY 8 cut(s) 120, 150, 243, 274, 287, 376, 434, 537
CviKI_1 RGCY 8 cut(s) 120, 150, 243, 274, 287, 376, 434, 537
DpnI GATC 3 cut(s) 19, 326, 581
DpnII GATC 3 cut(s) 17, 324, 579
Eam1104I CTCTTC 1 cut(s) 596
EarI CTCTTC 1 cut(s) 596
Eco24I GRGCYC 1 cut(s) 245
Eco31I GGTCTC 1 cut(s) 374
Eco47I GGWCC 1 cut(s) 170
Eco88I CYCGRG 1 cut(s) 56
Eco91I GGTNACC 2 cut(s) 299, 499
EcoO65I GGTNACC 2 cut(s) 299, 499
EcoRII CCWGG 1 cut(s) 269
EcoT38I GRGCYC 1 cut(s) 245
FaeI CATG 3 cut(s) 424, 566, 593
FaiI YATR 7 cut(s) 384, 393, 422, 492, 517, 564, 591
FalI AAGNNNNNCTT 2 cut(s) 271, 303
FaqI GGGAC 2 cut(s) 99, 156
FatI CATG 3 cut(s) 420, 562, 589
FauI CCCGC 1 cut(s) 258
Fnu4HI GCNGC 3 cut(s) 135, 187, 359
FokI GGATG 1 cut(s) 319
FriOI GRGCYC 1 cut(s) 245
Fsp4HI GCNGC 3 cut(s) 135, 187, 359
FspBI CTAG 1 cut(s) 417
FspI TGCGCA 1 cut(s) 313
GlaI GCGC 3 cut(s) 7, 313, 554
GluI GCNGC 3 cut(s) 135, 187, 359
HaeIII GGCC 2 cut(s) 120, 274
HgaI GACGC 3 cut(s) 114, 166, 191
HhaI GCGC 3 cut(s) 8, 314, 555
Hin1I GRCGYC 3 cut(s) 125, 177, 202
Hin1II CATG 3 cut(s) 424, 566, 593
Hin6I GCGC 3 cut(s) 6, 312, 553
HinP1I GCGC 3 cut(s) 6, 312, 553
HindIII AAGCTT 1 cut(s) 285
HinfI GANTC 2 cut(s) 164, 479
Hpy188I TCNGA 4 cut(s) 22, 207, 294, 529
Hpy188III TCNNGA 2 cut(s) 82, 265
HpyAV CCTTC 4 cut(s) 70, 108, 464, 535
HpyCH4V TGCA 3 cut(s) 134, 186, 441
HpyF10VI GCNNNNNNNGC 4 cut(s) 117, 131, 183, 320
Hsp92I GRCGYC 3 cut(s) 125, 177, 202
Hsp92II CATG 3 cut(s) 424, 566, 593
HspAI GCGC 3 cut(s) 6, 312, 553
Kzo9I GATC 3 cut(s) 17, 324, 579
Lsp1109I GCAGC 2 cut(s) 146, 198
MaeI CTAG 1 cut(s) 417
MaeIII GTNAC 2 cut(s) 299, 499
MalI GATC 3 cut(s) 19, 326, 581
MboI GATC 3 cut(s) 17, 324, 579
MhlI GDGCHC 1 cut(s) 245
MluCI AATT 1 cut(s) 379
MlyI GAGTC 1 cut(s) 173
MnlI CCTC 6 cut(s) 19, 101, 256, 264, 325, 597
MroXI GAANNNNTTC 1 cut(s) 545
MspA1I CMGCKG 1 cut(s) 215
MspR9I CCNGG 5 cut(s) 103, 117, 122, 174, 271
MvaI CCWGG 1 cut(s) 271
MvnI CGCG 1 cut(s) 8
MwoI GCNNNNNNNGC 4 cut(s) 117, 131, 183, 320
NciI CCSGG 4 cut(s) 103, 117, 122, 174
NdeII GATC 3 cut(s) 17, 324, 579
NlaIII CATG 3 cut(s) 424, 566, 593
NlaIV GGNNCC 1 cut(s) 172
NsbI TGCGCA 1 cut(s) 313
PdmI GAANNNNTTC 1 cut(s) 545
PfeI GAWTC 1 cut(s) 479
PkrI GCNGC 3 cut(s) 136, 188, 360
Ple19I CGATCG 1 cut(s) 327
PleI GAGTC 1 cut(s) 172
PpsI GAGTC 1 cut(s) 172
PsiI TTATAA 1 cut(s) 393
Psp6I CCWGG 1 cut(s) 269
PspEI GGTNACC 2 cut(s) 299, 499
PspGI CCWGG 1 cut(s) 269
PspN4I GGNNCC 1 cut(s) 172
PspPI GGNCC 2 cut(s) 119, 170
PsrI GAACNNNNNNTAC 2 cut(s) 220, 252
PvuI CGATCG 1 cut(s) 327
SatI GCNGC 3 cut(s) 135, 187, 359
Sau3AI GATC 3 cut(s) 17, 324, 579
Sau96I GGNCC 2 cut(s) 119, 170
SchI GAGTC 1 cut(s) 173
ScrFI CCNGG 5 cut(s) 103, 117, 122, 174, 271
SduI GDGCHC 1 cut(s) 245
SetI ASST 6 cut(s) 81, 93, 289, 515, 539, 608
SinI GGWCC 1 cut(s) 170
SmlI CTYRAG 1 cut(s) 371
SmoI CTYRAG 1 cut(s) 371
Sse9I AATT 1 cut(s) 379
SsiI CCGC 3 cut(s) 215, 251, 359
SspMI CTAG 1 cut(s) 417
StyD4I CCNGG 5 cut(s) 101, 115, 120, 172, 269
TaqI TCGA 2 cut(s) 239, 327
TasI AATT 1 cut(s) 379
TauI GCSGC 1 cut(s) 361
TfiI GAWTC 1 cut(s) 479
TseI GCWGC 2 cut(s) 134, 186
TspDTI ATGAA 1 cut(s) 399
TspGWI ACGGA 1 cut(s) 215
VpaK11BI GGWCC 1 cut(s) 170
XmnI GAANNNNTTC 1 cut(s) 545
XspI CTAG 1 cut(s) 417
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.