MD13G1136000.v1.1

Cation H( ) antiporter

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr13
OG0004570
Physical Location & Seq
Reverse (-)
10472151 .. 10475248
3098 bp
Loading structure...
UTR
Exon/CDS
Intron
MD13G1136000.v1.1.491

Sequence Viewer

Length: 2454 bp
ATGGCTCCGCCGCCACCGCTGGTTGCCCCCATGAAGGCAACATCCAATGGATCGTTTCAAGGCGAAAATCCCCTGGATTTTGCTCTTCCCTTACTCATTCTCCAGATTGTCTTGGTCGTTGTTTTCACAAGAACACTTGCATTCCTTCTTAGACCTCTCCGGCAACCTCGAGTCATAGCCGAAGTCATTGGAGGGATATTGCTTGGACCGTCGGCATTGGGGAGAAGCCAGAAGTTTCTACACACTGTTTTCCCGGCAAAAGCCATGACCGTTCTGGACACCGTGGCAAATATCGGACTCCTCTTCTTCTTGTTCCTCGTCGGCCTTGAACTCGACATCCGCGCCATTCGACGCACCGGCAAGAAATCCCTAGGCATCGCCGTCGCTGGAATCACCCTCCCTTTCGTCCTTGGCGTCTTTACCTCCTTCGCCCTCCGCGCCACTGTCTCCAAAGGCGTCAGCCAAGGCCCCTTCCTCGTCTTCATCGGCGTCGCCCTCTCCATCACAGCCTTCCCCGTCCTCGCCCGCATCCTCGCCGAGCTGAAGCTCCTCACCACCGACGTCGGCCGCATTGCCATGTCGGCCGCCGCTGTGAACGACGTCGCCGCCTGGATCCTCCTCGCCCTCGCTATCGCCCTCACCGGCACAAACACCTCCCCTCTCGTCTCCGTGTGGGTCCTACTCTCCGGGGCCTGCTTCGTCGGCTTCTGCATCTTCGCCCTAAGACCGCTCCTCGTCATGATGGCGAGGCGGTCACCCGACGGCGAGCCGGTGAAGGAGCTCTACATTTGCATCACGTTGTCCCTCGTCCTGGCGGCGAGCTTCGTGACGGACGTGATTGGGATCCACGCACTGTTTGGCGCGTTCGTGGTGGGGATAATAGTGCCGAAGGACGGCCCGTTCGCCGGGGTGCTGATAGAGAAGATAGAGGATCTGGTGTCGGGGCTTTTCTTGCCGCTGTATTTCGTGTCGAGCGGGCTGAAGACCAACGTGGCGACCATCAGCGGCGGGCAGTCGTGGGCGCTGCTGCTGCTGGTTATATTCACCGCGTCTTTTGGGAAGATTGTGGGGACGGTGAGTTTGTCGATGGCGTGTAAGGTGCCGTTCAGGGAGGCGTTGGCGCTTGGGTTTCTGATGAACACCAAGGGGTTGGTGGAGCTCATCGTCCTCAACATTGGCAAGGATAGAAAGGTGCTGAACGACCAAACCTTCGCCATATTCGTCCTAATGGCATTATTCACCACCTTCATCACCACCCCACTAGTGGTGGCCATCTACAAGCCAGCCCGCCGAGGCGCCCCCTACAAGCACCGCAGCGTCTTCCGCAAGAACCCCGACACGGAGCTCCGAATGCTTGCCTGCTTCCACAGCACCCGCAACATCCCCACCATGATCAACCTCATCGAGTCCTCCAGGGGGACCCGCAAGCGCGGCCGCCTCACCGTCTACGCCATGCACCTCATGGAGCTTTCGGAGCGCTCCTCCGCCATCTCCATGGTCCACAAAGCCCGCCACAACGGCCTCCCCTTCTGGAACAAAAAAACCGACGAAAACAACAAGGACCAAATGGTCATTGCATTCGAGGCCTACGAGCAGCTCAGCTCCGTCAAGGTCCGCCCCATGACCGCCATCTCCTCCCTCAGCGACATTCACGAGGACATCTGCGCCAGCGCCCACCAAAAGAACACAGCCGTGATTTTACTCCCGTTTCACAAACACCAGAGGCTCGACGGCACCATGGAGTCTTTAGGAAACGCACTTCGTTCGGTCAACGAGCGGGTGCTCAAACACGCTCCCTGTTCGGTTGGGATTTTGGTGGACAGGGGTCTTGGCGGGACAGCCCAGGTCTCGGCCAGCGATGTGTCGTATAATATTGTGGTGGCGTTTTTCGGAGGGCGGGACGACAGGGAGGCGTTGGCTTACGGAATGAGGCTGGCGGAGCATCCGGGGATTGTGATGACGTTGATTCGGTTTGTGGCCCCAGAAGGGAAGACGTTGAGATTTGGCGCGAAATTGGTCGGGATAACTTCCGACAAGAATATGAAGATTTTGGAAGAGGAAGACTGCGGAGGCGACGAGAAGGAGAACGACGAGTCGCTTTTGGCGGAGTTTATGAATGTGAGGAAGAATAGTGGGAACAAGGAGGGCGGCGGGGAGCCGTCGATGTTGTACGAGGAGAAGGTGGTGGATAGCAAGGCGGAGATTTGTGTCGTGTTGAAATCGATGGCTAAAAATGTCAATTTGTTTATTGTGGGGAGAATGCCTCCGACGGCGCCATTGGTGAACAGTTCGAGTGACTGCGCGGAGTTGGGGCCGGTGGGGAGCTTCTTGGCGTCGTCGGACTTTTCGTCCACGGCATCGGTTATGGTGCTTCAACAGTACAATCCCACGGCGTCACAGCCGCTTGTGGTGGAGGAAGCTGATTGTGAGGCGCCGGATACACCAATAGCTTGA

Protein Analysis

818

Amino Acids

88.14

Weight (kDa)

8.94

Isoelectric Point (pI)

38.69

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Na_H_Exchanger PF00999 41 - 421 2.3e-63 Sodium/hydrogen exchanger, transmembrane
CHX17_2nd PF23256 477 - 619 4.5e-54 Plant cation/H(+) antiporter domain
CHX17_C PF23259 624 - 664 2.6e-09 Plant cation/H(+) antiporter, C-terminal domain
CHX17_C PF23259 723 - 795 6.4e-06 Plant cation/H(+) antiporter, C-terminal domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 1568
AatII GACGTC 2 cut(s) 564, 603
AccB1I GGYRCC 5 cut(s) 1099, 1295, 1733, 2272, 2431
AccBSI CCGCTC 3 cut(s) 730, 975, 1777
AccI GTMKAC 1 cut(s) 1446
AccII CGCG 7 cut(s) 342, 438, 863, 1049, 1431, 2009, 2303
AclWI GGATC 6 cut(s) 58, 607, 620, 838, 851, 939
AcoI YGGCCR 5 cut(s) 565, 582, 1269, 1432, 1851
AcuI CTGAAG 2 cut(s) 563, 1001
AfaI GTAC 2 cut(s) 2171, 2381
AfeI AGCGCT 1 cut(s) 1478
AfiI CCNNNNNNNGG 9 cut(s) 34, 811, 893, 905, 1264, 1339, 1416, 1849, 1986
AgsI TTSAA 4 cut(s) 59, 329, 2218, 2375
AhdI GACNNNNNGTC 1 cut(s) 2347
AhlI ACTAGT 1 cut(s) 1261
AjiI CACGTC 1 cut(s) 835
AjnI CCWGG 5 cut(s) 72, 608, 810, 1412, 1842
AleI CACNNNNGTG 1 cut(s) 1691
AloI GAACNNNNNNTCC 4 cut(s) 321, 353, 884, 916
Alw21I GWGCWC 4 cut(s) 783, 1161, 1347, 1785
Alw26I GTCTC 3 cut(s) 451, 670, 1852
AlwI GGATC 6 cut(s) 58, 607, 620, 838, 851, 939
Ama87I CYCGRG 1 cut(s) 168
Aor51HI AGCGCT 1 cut(s) 1478
ApeKI GCWGC 5 cut(s) 1024, 1027, 1030, 1314, 1594
ArsI GACNNNNNNTTYG 6 cut(s) 884, 916, 1063, 1095, 1193, 1225
AspA2I CCTAGG 1 cut(s) 370
AsuC2I CCSGG 4 cut(s) 254, 688, 907, 1947
AvaI CYCGRG 1 cut(s) 168
AvaII GGWCC 6 cut(s) 206, 676, 1419, 1498, 1561, 1612
AvrII CCTAGG 1 cut(s) 370
BalI TGGCCA 1 cut(s) 1271
BamHI GGATCC 2 cut(s) 612, 843
BanI GGYRCC 5 cut(s) 1099, 1295, 1733, 2272, 2431
BanII GRGCYC 3 cut(s) 783, 1161, 1347
BarI GAAGNNNNNNTAC 2 cut(s) 767, 799
BauI CACGAG 1 cut(s) 1652
BbsI GAAGAC 5 cut(s) 472, 989, 1312, 1997, 2067
Bbv12I GWGCWC 4 cut(s) 783, 1161, 1347, 1785
BbvCI CCTCAGC 1 cut(s) 1640
BbvI GCAGC 5 cut(s) 1011, 1014, 1017, 1326, 1606
BccI CCATC 8 cut(s) 509, 736, 1007, 1081, 1280, 1496, 1637, 2218
BcgI CGANNNNNNTGC 4 cut(s) 364, 398, 1746, 1780
BciT130I CCWGG 5 cut(s) 74, 610, 812, 1414, 1844
BciVI GTATCC 1 cut(s) 2431
BclI TGATCA 1 cut(s) 1392
BcnI CCSGG 4 cut(s) 254, 688, 907, 1947
BcoDI GTCTC 3 cut(s) 451, 670, 1852
BcuI ACTAGT 1 cut(s) 1261
BfaI CTAG 2 cut(s) 371, 1262
BfoI RGCGCY 7 cut(s) 1025, 1124, 1299, 1480, 1674, 2276, 2435
BfuI GTATCC 1 cut(s) 2431
BglI GCCNNNNNGGC 2 cut(s) 581, 1518
BlnI CCTAGG 1 cut(s) 370
BlpI GCTNAGC 1 cut(s) 1598
Bme1390I CCNGG 9 cut(s) 74, 254, 610, 688, 812, 907, 1414, 1844, 1947
Bme18I GGWCC 6 cut(s) 206, 676, 1419, 1498, 1561, 1612
BmeRI GACNNNNNGTC 1 cut(s) 2347
BmeT110I CYCGRG 1 cut(s) 168
BmgBI CACGTC 1 cut(s) 835
BmrFI CCNGG 9 cut(s) 74, 254, 610, 688, 812, 907, 1414, 1844, 1947
BmsI GCATC 6 cut(s) 384, 537, 720, 801, 1951, 2366
BoxI GACNNNNGTC 1 cut(s) 1824
BpiI GAAGAC 5 cut(s) 472, 989, 1312, 1997, 2067
BplI GAGNNNNNCTC 4 cut(s) 1466, 1498, 2248, 2280
BpmI CTGGAG 2 cut(s) 86, 1396
Bpu10I CCTNAGC 1 cut(s) 1640
Bpu1102I GCTNAGC 1 cut(s) 1598
BpuMI CCSGG 4 cut(s) 254, 688, 907, 1947
Bsa29I ATCGAT 1 cut(s) 2222
BsaBI GATNNNNATC 1 cut(s) 842
BsaI GGTCTC 1 cut(s) 1852
BsaXI ACNNNNNCTCC 2 cut(s) 84, 114
Bsc4I CCNNNNNNNGG 9 cut(s) 34, 811, 893, 905, 1264, 1339, 1416, 1849, 1986
Bse118I RCCGGY 4 cut(s) 356, 641, 769, 2314
Bse3DI GCAATG 2 cut(s) 570, 1572
Bse8I GATNNNNATC 1 cut(s) 842
BseBI CCWGG 5 cut(s) 74, 610, 812, 1414, 1844
BseCI ATCGAT 1 cut(s) 2222
BseGI GGATG 5 cut(s) 41, 336, 528, 1380, 1942
BseJI GATNNNNATC 1 cut(s) 842
BseLI CCNNNNNNNGG 9 cut(s) 34, 811, 893, 905, 1264, 1339, 1416, 1849, 1986
BseMI GCAATG 2 cut(s) 570, 1572
BseMII CTCAG 2 cut(s) 1612, 1654
BseRI GAGGAG 7 cut(s) 290, 539, 608, 722, 1471, 1624, 2189
BseX3I CGGCCG 3 cut(s) 565, 582, 1432
BseXI GCAGC 5 cut(s) 1011, 1014, 1017, 1326, 1606
Bsh1236I CGCG 7 cut(s) 342, 438, 863, 1049, 1431, 2009, 2303
Bsh1285I CGRYCG 3 cut(s) 568, 585, 1435
BshNI GGYRCC 5 cut(s) 1099, 1295, 1733, 2272, 2431
BshVI ATCGAT 1 cut(s) 2222
BsiEI CGRYCG 3 cut(s) 568, 585, 1435
BsiHKAI GWGCWC 4 cut(s) 783, 1161, 1347, 1785
BsiHKCI CYCGRG 1 cut(s) 168
BslFI GGGAC 5 cut(s) 787, 1084, 1432, 1849, 1913
BslI CCNNNNNNNGG 9 cut(s) 34, 811, 893, 905, 1264, 1339, 1416, 1849, 1986
BsmAI GTCTC 3 cut(s) 451, 670, 1852
BsmBI CGTCTC 1 cut(s) 670
BsmFI GGGAC 5 cut(s) 787, 1084, 1432, 1849, 1913
BsmI GAATGC 4 cut(s) 140, 1356, 1577, 2265
Bso31I GGTCTC 1 cut(s) 1852
BsoBI CYCGRG 1 cut(s) 168
Bsp1286I GDGCHC 4 cut(s) 783, 1161, 1347, 1785
Bsp143I GATC 5 cut(s) 50, 612, 843, 931, 1392
Bsp1720I GCTNAGC 1 cut(s) 1598
Bsp19I CCATGG 2 cut(s) 1494, 1737
BspCNI CTCAG 2 cut(s) 1611, 1653
BspDI ATCGAT 1 cut(s) 2222
BspFNI CGCG 7 cut(s) 342, 438, 863, 1049, 1431, 2009, 2303
BspHI TCATGA 1 cut(s) 738
BspPI GGATC 6 cut(s) 58, 607, 620, 838, 851, 939
BspQI GCTCTTC 1 cut(s) 90
BspT107I GGYRCC 5 cut(s) 1099, 1295, 1733, 2272, 2431
BspTNI GGTCTC 1 cut(s) 1852
BsrBI CCGCTC 3 cut(s) 730, 975, 1777
BsrDI GCAATG 2 cut(s) 570, 1572
BsrFI RCCGGY 4 cut(s) 356, 641, 769, 2314
BssAI RCCGGY 4 cut(s) 356, 641, 769, 2314
BssMI GATC 5 cut(s) 50, 612, 843, 931, 1392
BssSI CACGAG 1 cut(s) 1652
BssT1I CCWWGG 6 cut(s) 370, 409, 463, 1143, 1494, 1737
Bst2BI CACGAG 1 cut(s) 1652
Bst2UI CCWGG 5 cut(s) 74, 610, 812, 1414, 1844
Bst6I CTCTTC 3 cut(s) 90, 308, 2049
BstDEI CTNAG 4 cut(s) 149, 722, 1598, 1640
BstDSI CCRYGG 5 cut(s) 282, 1494, 1737, 2352, 2388
BstEII GGTNACC 1 cut(s) 753
BstF5I GGATG 5 cut(s) 41, 336, 528, 1380, 1942
BstFNI CGCG 7 cut(s) 342, 438, 863, 1049, 1431, 2009, 2303
BstH2I RGCGCY 7 cut(s) 1025, 1124, 1299, 1480, 1674, 2276, 2435
BstKTI GATC 5 cut(s) 53, 615, 846, 934, 1395
BstMAI GTCTC 3 cut(s) 451, 670, 1852
BstMBI GATC 5 cut(s) 50, 612, 843, 931, 1392
BstMCI CGRYCG 3 cut(s) 568, 585, 1435
BstNI CCWGG 5 cut(s) 74, 610, 812, 1414, 1844
BstPAI GACNNNNGTC 1 cut(s) 1824
BstPI GGTNACC 1 cut(s) 753
BstSCI CCNGG 9 cut(s) 72, 252, 608, 686, 810, 905, 1412, 1842, 1945
BstUI CGCG 7 cut(s) 342, 438, 863, 1049, 1431, 2009, 2303
BstV1I GCAGC 5 cut(s) 1011, 1014, 1017, 1326, 1606
BstV2I GAAGAC 5 cut(s) 472, 989, 1312, 1997, 2067
BstX2I RGATCY 3 cut(s) 612, 843, 931
BstXI CCANNNNNNTGG 2 cut(s) 1150, 1495
BstYI RGATCY 3 cut(s) 612, 843, 931
BstZI CGGCCG 3 cut(s) 565, 582, 1432
Bsu15I ATCGAT 1 cut(s) 2222
BsuI GTATCC 1 cut(s) 2431
BsuTUI ATCGAT 1 cut(s) 2222
BtgI CCRYGG 5 cut(s) 282, 1494, 1737, 2352, 2388
BtgZI GCGATG 2 cut(s) 361, 1872
BtrI CACGTC 1 cut(s) 835
BtsCI GGATG 5 cut(s) 41, 336, 528, 1380, 1942
BtsIMutI CAGTG 3 cut(s) 243, 441, 851
CciI TCATGA 1 cut(s) 738
CciNI GCGGCCGC 1 cut(s) 1432
Cfr10I RCCGGY 4 cut(s) 356, 641, 769, 2314
ClaI ATCGAT 1 cut(s) 2222
CseI GACGC 8 cut(s) 360, 403, 445, 478, 1038, 1306, 2322, 2382
Csp6I GTAC 2 cut(s) 2170, 2380
CviQI GTAC 2 cut(s) 2170, 2380
DdeI CTNAG 4 cut(s) 149, 722, 1598, 1640
DinI GGCGCC 3 cut(s) 1297, 2274, 2433
DpnI GATC 5 cut(s) 52, 614, 845, 933, 1394
DpnII GATC 5 cut(s) 50, 612, 843, 931, 1392
DrdI GACNNNNNNGTC 1 cut(s) 1568
DriI GACNNNNNGTC 1 cut(s) 2347
DseDI GACNNNNNNGTC 1 cut(s) 1568
EaeI YGGCCR 5 cut(s) 565, 582, 1269, 1432, 1851
EagI CGGCCG 3 cut(s) 565, 582, 1432
Eam1104I CTCTTC 3 cut(s) 90, 308, 2049
Eam1105I GACNNNNNGTC 1 cut(s) 2347
EarI CTCTTC 3 cut(s) 90, 308, 2049
EciI GGCGGA 5 cut(s) 1474, 1604, 1952, 2120, 2213
Ecl136II GAGCTC 3 cut(s) 781, 1159, 1345
EclXI CGGCCG 3 cut(s) 565, 582, 1432
Eco130I CCWWGG 6 cut(s) 370, 409, 463, 1143, 1494, 1737
Eco147I AGGCCT 1 cut(s) 1586
Eco24I GRGCYC 3 cut(s) 783, 1161, 1347
Eco31I GGTCTC 1 cut(s) 1852
Eco47I GGWCC 6 cut(s) 206, 676, 1419, 1498, 1561, 1612
Eco47III AGCGCT 1 cut(s) 1478
Eco52I CGGCCG 3 cut(s) 565, 582, 1432
Eco53kI GAGCTC 3 cut(s) 781, 1159, 1345
Eco57I CTGAAG 2 cut(s) 563, 1001
Eco88I CYCGRG 1 cut(s) 168
Eco91I GGTNACC 1 cut(s) 753
EcoICRI GAGCTC 3 cut(s) 781, 1159, 1345
EcoO109I RGGNCCY 4 cut(s) 467, 676, 690, 1419
EcoO65I GGTNACC 1 cut(s) 753
EcoRII CCWGG 5 cut(s) 72, 608, 810, 1412, 1842
EcoT14I CCWWGG 6 cut(s) 370, 409, 463, 1143, 1494, 1737
EcoT38I GRGCYC 3 cut(s) 783, 1161, 1347
EgeI GGCGCC 3 cut(s) 1297, 2274, 2433
EheI GGCGCC 3 cut(s) 1297, 2274, 2433
ErhI CCWWGG 6 cut(s) 370, 409, 463, 1143, 1494, 1737
Esp3I CGTCTC 1 cut(s) 670
FaqI GGGAC 5 cut(s) 787, 1084, 1432, 1849, 1913
FbaI TGATCA 1 cut(s) 1392
FblI GTMKAC 1 cut(s) 1446
FokI GGATG 5 cut(s) 28, 323, 515, 1367, 1929
FriOI GRGCYC 3 cut(s) 783, 1161, 1347
FspBI CTAG 2 cut(s) 371, 1262
GsuI CTGGAG 2 cut(s) 86, 1396
HaeII RGCGCY 7 cut(s) 1025, 1124, 1299, 1480, 1674, 2276, 2435
HgaI GACGC 8 cut(s) 360, 403, 445, 478, 1038, 1306, 2322, 2382
HincII GTYRAC 1 cut(s) 1771
HindII GTYRAC 1 cut(s) 1771
HinfI GANTC 7 cut(s) 171, 297, 390, 1406, 1742, 1966, 2093
Hpy166II GTNNAC 7 cut(s) 595, 1447, 1501, 1771, 1819, 2284, 2352
Hpy188I TCNGA 8 cut(s) 296, 1134, 1349, 1474, 1892, 2032, 2268, 2341
Hpy188III TCNNGA 7 cut(s) 103, 275, 739, 826, 1531, 1652, 2020
Hpy8I GTNNAC 7 cut(s) 595, 1447, 1501, 1771, 1819, 2284, 2352
HpyCH4IV ACGT 7 cut(s) 561, 600, 797, 834, 990, 1961, 1994
HpyCH4V TGCA 5 cut(s) 140, 711, 792, 1456, 1577
HpyF3I CTNAG 4 cut(s) 149, 722, 1598, 1640
HpySE526I ACGT 7 cut(s) 561, 600, 797, 834, 990, 1961, 1994
KasI GGCGCC 3 cut(s) 1295, 2272, 2431
KflI GGGWCCC 1 cut(s) 1419
Ksp22I TGATCA 1 cut(s) 1392
Kzo9I GATC 5 cut(s) 50, 612, 843, 931, 1392
LguI GCTCTTC 1 cut(s) 90
Lsp1109I GCAGC 5 cut(s) 1011, 1014, 1017, 1326, 1606
LweI GCATC 6 cut(s) 384, 537, 720, 801, 1951, 2366
MaeI CTAG 2 cut(s) 371, 1262
MaeII ACGT 7 cut(s) 561, 600, 797, 834, 990, 1961, 1994
MaeIII GTNAC 4 cut(s) 753, 826, 2294, 2394
MalI GATC 5 cut(s) 52, 614, 845, 933, 1394
MbiI CCGCTC 3 cut(s) 730, 975, 1777
MboI GATC 5 cut(s) 50, 612, 843, 931, 1392
MflI RGATCY 3 cut(s) 612, 843, 931
MhlI GDGCHC 4 cut(s) 783, 1161, 1347, 1785
MlsI TGGCCA 1 cut(s) 1271
MluCI AATT 2 cut(s) 2012, 2239
MluNI TGGCCA 1 cut(s) 1271
Mly113I GGCGCC 3 cut(s) 1296, 2273, 2432
MlyI GAGTC 5 cut(s) 180, 291, 1415, 1751, 2102
MmeI TCCRAC 3 cut(s) 2055, 2291, 2319
Mox20I TGGCCA 1 cut(s) 1271
MscI TGGCCA 1 cut(s) 1271
MslI CAYNNNNRTG 3 cut(s) 575, 1493, 1691
Msp20I TGGCCA 1 cut(s) 1271
MspA1I CMGCKG 4 cut(s) 19, 590, 958, 1005
MspR9I CCNGG 9 cut(s) 74, 254, 610, 688, 812, 907, 1414, 1844, 1947
Mva1269I GAATGC 4 cut(s) 140, 1356, 1577, 2265
MvaI CCWGG 5 cut(s) 74, 610, 812, 1414, 1844
MvnI CGCG 7 cut(s) 342, 438, 863, 1049, 1431, 2009, 2303
NarI GGCGCC 3 cut(s) 1296, 2273, 2432
NciI CCSGG 4 cut(s) 254, 688, 907, 1947
NcoI CCATGG 2 cut(s) 1494, 1737
NdeII GATC 5 cut(s) 50, 612, 843, 931, 1392
NmeAIII GCCGAG 3 cut(s) 562, 1316, 1829
NmuCI GTSAC 4 cut(s) 753, 826, 2294, 2394
NotI GCGGCCGC 1 cut(s) 1432
OliI CACNNNNGTG 1 cut(s) 1691
PaeR7I CTCGAG 1 cut(s) 168
PagI TCATGA 1 cut(s) 738
PceI AGGCCT 1 cut(s) 1586
PciSI GCTCTTC 1 cut(s) 90
PcsI WCGNNNNNNNCGW 5 cut(s) 411, 831, 1218, 1587, 2345
PctI GAATGC 4 cut(s) 140, 1356, 1577, 2265
PfeI GAWTC 2 cut(s) 390, 1966
PleI GAGTC 5 cut(s) 179, 291, 1414, 1750, 2101
PluTI GGCGCC 3 cut(s) 1299, 2276, 2435
PpsI GAGTC 5 cut(s) 179, 291, 1414, 1750, 2101
PpuMI RGGWCCY 2 cut(s) 676, 1419
PshAI GACNNNNGTC 1 cut(s) 1824
Psp124BI GAGCTC 3 cut(s) 783, 1161, 1347
Psp5II RGGWCCY 2 cut(s) 676, 1419
Psp6I CCWGG 5 cut(s) 72, 608, 810, 1412, 1842
PspEI GGTNACC 1 cut(s) 753
PspGI CCWGG 5 cut(s) 72, 608, 810, 1412, 1842
PspPPI RGGWCCY 2 cut(s) 676, 1419
PspXI VCTCGAGB 1 cut(s) 168
PsuI RGATCY 3 cut(s) 612, 843, 931
RsaI GTAC 2 cut(s) 2171, 2381
RsaNI GTAC 2 cut(s) 2170, 2380
RseI CAYNNNNRTG 3 cut(s) 575, 1493, 1691
SacI GAGCTC 3 cut(s) 783, 1161, 1347
SapI GCTCTTC 1 cut(s) 90
Sau3AI GATC 5 cut(s) 50, 612, 843, 931, 1392
SchI GAGTC 5 cut(s) 180, 291, 1415, 1751, 2102
ScrFI CCNGG 9 cut(s) 74, 254, 610, 688, 812, 907, 1414, 1844, 1947
SduI GDGCHC 4 cut(s) 783, 1161, 1347, 1785
SfaNI GCATC 6 cut(s) 384, 537, 720, 801, 1951, 2366
SfoI GGCGCC 3 cut(s) 1297, 2274, 2433
Sfr274I CTCGAG 1 cut(s) 168
SinI GGWCC 6 cut(s) 206, 676, 1419, 1498, 1561, 1612
SlaI CTCGAG 1 cut(s) 168
SmiMI CAYNNNNRTG 3 cut(s) 575, 1493, 1691
SmlI CTYRAG 1 cut(s) 168
SmoI CTYRAG 1 cut(s) 168
SpeI ACTAGT 1 cut(s) 1261
Sse9I AATT 2 cut(s) 2012, 2239
SseBI AGGCCT 1 cut(s) 1586
SspDI GGCGCC 3 cut(s) 1295, 2272, 2431
SspI AATATT 1 cut(s) 1873
SspMI CTAG 2 cut(s) 371, 1262
SstI GAGCTC 3 cut(s) 783, 1161, 1347
StuI AGGCCT 1 cut(s) 1586
StyD4I CCNGG 9 cut(s) 72, 252, 608, 686, 810, 905, 1412, 1842, 1945
StyI CCWWGG 6 cut(s) 370, 409, 463, 1143, 1494, 1737
TaiI ACGT 7 cut(s) 564, 603, 800, 837, 993, 1964, 1997
TaqII GACCGA 1 cut(s) 1756
TasI AATT 2 cut(s) 2012, 2239
TatI WGTACW 1 cut(s) 2379
TfiI GAWTC 2 cut(s) 390, 1966
TscAI CASTG 3 cut(s) 250, 448, 858
TseFI GTSAC 4 cut(s) 753, 826, 2294, 2394
TseI GCWGC 5 cut(s) 1024, 1027, 1030, 1314, 1594
Tsp45I GTSAC 4 cut(s) 753, 826, 2294, 2394
TspDTI ATGAA 6 cut(s) 47, 472, 1151, 1237, 2057, 2129
TspGWI ACGGA 5 cut(s) 658, 845, 1355, 1594, 1938
TspRI CASTG 3 cut(s) 250, 448, 858
VpaK11BI GGWCC 6 cut(s) 206, 676, 1419, 1498, 1561, 1612
XcmI CCANNNNNNNNNTGG 5 cut(s) 271, 854, 1150, 1261, 1459
XhoI CTCGAG 1 cut(s) 168
XmaJI CCTAGG 1 cut(s) 370
XmiI GTMKAC 1 cut(s) 1446
XspI CTAG 2 cut(s) 371, 1262
ZraI GACGTC 2 cut(s) 562, 601
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.