Basic Information

Type: gene

Biological Identity

Species

rosa_wichuraiana

Chromosome

Chr3

Orthogroup ID

OG0004570

Physical Location & Seq

Genomic Coordinates Reverse (-)

29076691 .. 29080152

Gene Structure 3462 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

Rw3G023230.1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2433 bp

ATGGCACCTGCGCCGCTGCCGGCAACCACCCCACCCATGAAGGCCACTTCAAATGGATCATTTCAAGGTGAAAATCCACTTGATTACGCTCTTCCCTTACTGATCCTCCAAATCATCTTGGTCGTGGTGTTCACCAGGTTTCTTGCATACCTTCTAAAACCTCTCCGGCAACCCCGAGTCATAGCCGAAGTTATAGGCGGATGTCTGCTGGGCCCCTCCGCATTCGGGAGGAGCAAGACCTTTCTACACATGATTTTCCCCCCAAAAGGCATGACGGTGCTAGACACGGTAGCCAACATCGGGCTCTGCTTCTTCCTCTTCCTCGTCGGCCTTGAGCTCGACATCGGCGCCATCCGCCGCACAGGCAAGAAGTCCCTGGGCATCGCCTTCTGCGGGATCACCCTCCCCTTCGTCCTCGGCATCGGCACCTCCGAAGCCCTACGCGCCACTGTCTCCAAAGGCGTGAAACAAGGCCCCTTCCTCGTTTTCAACGGCGTCGCCCTCTCCATTACCGCCTTCCCCGTCCTCGCCCGCATCCTCGCTGAGCTCAAGCTCCTCACCACCGACGTCGGCCGCATCGCCATGTCCGCCGCCGCCGTCAACGACGTCGCCGCCTGGATCCTTCTAGCCCTCGCCATCGCCCTCACCGGCAACAACACCTCCCCGCTAGTCTCCGTCTGGGTCCTCCTCTGCGGTGCCGGCTTCGTCGGGTTCGCCGTCGTCGCCATCCGCCCCATACTGGTCATGATGGCGCGAAGGTCCCAGGAGGGCGAACCCGTGAAGGAGCTCTACATCTGCATCACTCTCTCGCTGGTTTTGGCCGCGGGGTTCGTGACGGACACGATTGGGATACACGCGCTGTTCGGCGCGTTCGTGGTGGGGATCATAATGCCGAAGGAGGGTCCGTTCGCTGGTGTGTTGATAGAGAAGATTGAGGACCTGGTTTCGGGGCTTCTATTGCCGCTTTATTTTGTATCCAGTGGGCTCAAGACTAATTTTTTGACTATAAGCGGGGGGGTGTCGTGGGGTATGCTGGTGCTGGTGATATGTACAGCGTCGTTTGGGAAGATCGTCGGGACTGTCACCGTGTCGATGCTGTTCAAGGTGCCTTTCAGGGAAGCCTTGGCGCTTGGCTTCCTTATGAATACAAAGGGCTTGGTGGAGCTCATCGTCCTCAATATTGGCAAGGATAGAAAGGTACTGAACGAACAGACTTTTGCCATCTTTGTTTTGATGGCGTTGTTCACCACCTTCATCACCACACCACTAGTGATGGCAGTGTACAAACCAGCAAGAAAGGGAGCCCCTTACAAACTACGCACCATTTTTCGCAAAAGCCCAGACACAGAACTTCGAATATTGGCATGCTTCCACACCACCCGCAACATCCCCACCATTATCAACCTCATCGAGTCGTCTCGCGGCACCCACAAGCGCGGGCGACTCACCATCTACGCCATGCACCTCATGGAGCTCTCGGAGCGGTCCTCTGCAATCTCCATGGTCCACAAAGCCCGCTATAATGGCCTCCCATTTTGGAACAAGAAGAGTGATGGAAATGACCAAATGGTTATTGCTTTTGAGGCCTACGAGCAGCTCAGCAGCGTCAAGATTCGGCCAGCCACCGCCATCTCTTCTTTAAACGACATGCACGAGGACATATGCGCCTGCGCGCACCAAAAAACTGTGGCAATGATTTTGTTGCCGTTTCACAAGCATCAGAAGCTGGACGGCACCATGGAGTCGGTGGGACACTCATTTCAAGTGGTGAACGAGCGTGTGCTCCGACATGCGCCTTGTTCGGTTGGGATTCTAGTTGATAGAGGACTCGGTGGCACCACGCAGGTCTCAGCTAGCGAAGTGTCGTATACGGCGGTGGTGGCGTTCTTCGGAGGAAGAGATGACCGGGAGGCACTGGCTTATGGTATGAGACTGGCTGAACATCCTGGGATTGCGTTGACTGTTTTTAGATTCGTTGCTTCACAAGGGAAGACATTGAGATTTGGAGCGAAATTAGTTGGGATAACAGCTGACAACAAGAAAATACTGAAAGAAGAAGATAGTAGCCAAGAGAATGAAGAGGACGAGAAGTTCTTGGAGGAGTACAAGAATGTAAGAAGTAGCAATAAGGAAAAGGAAGAGTCCATGTTTTACGAGGAGAAGGTGGTGGACAGCAAAGGGGAGATCGCGGTGGCATTAAAGACTCTGAGCAAGCACAACTTGTTTATAGTAGGGAGAATGTCGTCGACTGTCCCGTTGGTAGAGTCGAGTGACTGTGCAGAGCTTGGGCCAATCGGAAGCTTCTTAGCCTCGTCAGACTTCTCTTCCACAGCTTCAGTTGTGGTGATTCAACAATATGATGCTACAGCGTCGCGGCCGCTTGTGGTGGAGGAAGCTGACTATGAGTTGCCGGAGTCGTCGTCTATGGTATAA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005768 GO:0005770 GO:0006810 GO:0006811 GO:0006812 GO:0006885 GO:0008150 GO:0012505 GO:0015672 GO:0031410 GO:0031982 GO:0042592 GO:0043226 GO:0043227 GO:0043229 GO:0044424 GO:0044444 GO:0044464 GO:0048878 GO:0050801 GO:0051179 GO:0051234 GO:0055067 GO:0055080 GO:0065007 GO:0065008 GO:0097708 GO:0098771

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

Na_H_Exchanger

Protein Analysis

810

Amino Acids

87.83

Weight (kDa)

8.75

Isoelectric Point (pI)

41.17

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Na_H_Exchanger	PF00999	44 - 424	3.3e-59	Sodium/hydrogen exchanger, transmembrane
CHX17_2nd	PF23256	479 - 619	2.6e-51	Plant cation/H(+) antiporter domain
CHX17_C	PF23259	625 - 787	6.4e-19	Plant cation/H(+) antiporter, C-terminal domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0004570)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT3G17630 AT3G17630
fragaria_vesca	FvH4_4g21880
malus_domestica	MD13G1136000.v1.1 MD16G1131400.v1.1
prunus_persica	Prupe.1G209400_v2.0.a1
pyrus_communis	pycom16g11580
rosa_chinensis	RchiOBHm_Chr3g0483861
rosa_laevigata	RLG00000007178 RLG00000023257
rosa_multiflora	Rmu_co8183874.1_g000001 Rmu_co8462775.1_g000001 Rmu_sc0004940.1_g000012 Rmu_sc0005901.1_g000001 Rmu_sc0009599.1_g000012
rosa_roxburghii	Rroxscaffold_5G00369850 Rroxscaffold_6G00398090
rosa_rugosa	Rorug03G0208200.1 Rorug03G0208300 Rorug04G0222700 Rorug04G0222800
rosa_samantha	Rh3AG257000 Rh3BG293400 Rh3CG291700 Rh3DG286700 Rh4AG279900 Rh4AG280100 Rh4BG285300 Rh4BG285500 Rh4CG300900
rosa_wichuraiana	Rw3G023230

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AarI	CACCTGC	1 cut(s)	16
AatII	GACGTC	2 cut(s)	570, 609
Acc36I	ACCTGC	2 cut(s)	16, 1834
AccB1I	GGYRCC	8 cut(s)	4, 347, 425, 695, 1105, 1424, 1733, 1835
AccBSI	CCGCTC	1 cut(s)	1483
AccI	GTMKAC	2 cut(s)	1868, 2246
AclWI	GGATC	6 cut(s)	64, 97, 404, 613, 626, 890
AcoI	YGGCCR	4 cut(s)	571, 819, 1616, 2375
AcuI	CTGAAG	1 cut(s)	2319
AcyI	GRCGYC	4 cut(s)	348, 495, 567, 606
AfaI	GTAC	4 cut(s)	1051, 1200, 1283, 2105
AfiI	CCNNNNNNNGG	8 cut(s)	225, 266, 300, 393, 739, 899, 911, 946
AgsI	TTSAA	6 cut(s)	51, 65, 490, 1102, 1763, 2351
AhlI	ACTAGT	1 cut(s)	1267
AjnI	CCWGG	6 cut(s)	134, 375, 614, 762, 939, 1945
AloI	GAACNNNNNNTCC	4 cut(s)	890, 922, 2075, 2107
Alw21I	GWGCWC	6 cut(s)	339, 549, 789, 1167, 1476, 1785
Alw26I	GTCTC	5 cut(s)	457, 676, 1422, 1852, 1924
AlwI	GGATC	6 cut(s)	64, 97, 404, 613, 626, 890
AlwNI	CAGNNNCTG	1 cut(s)	1726
Ama87I	CYCGRG	1 cut(s)	174
ApaI	GGGCCC	1 cut(s)	215
ApeKI	GCWGC	3 cut(s)	16, 1594, 1602
ArsI	GACNNNNNNTTYG	2 cut(s)	982, 1014
AsuC2I	CCSGG	1 cut(s)	1907
AsuII	TTCGAA	1 cut(s)	1354
AsuNHI	GCTAGC	1 cut(s)	1853
AvaI	CYCGRG	1 cut(s)	174
AvaII	GGWCC	6 cut(s)	682, 759, 902, 937, 1485, 1504
BaeGI	GKGCMC	1 cut(s)	215
BamHI	GGATCC	1 cut(s)	618
BanI	GGYRCC	8 cut(s)	4, 347, 425, 695, 1105, 1424, 1733, 1835
BanII	GRGCYC	9 cut(s)	215, 306, 339, 549, 789, 987, 1167, 1306, 1476
BarI	GAAGNNNNNNTAC	2 cut(s)	773, 805
BauI	CACGAG	1 cut(s)	1652
BbsI	GAAGAC	1 cut(s)	1997
Bbv12I	GWGCWC	6 cut(s)	339, 549, 789, 1167, 1476, 1785
BbvI	GCAGC	3 cut(s)	3, 1606, 1614
BceAI	ACGGC	6 cut(s)	508, 581, 701, 1690, 1747, 1887
BciT130I	CCWGG	6 cut(s)	136, 377, 616, 764, 941, 1947
BciVI	GTATCC	2 cut(s)	843, 985
BcnI	CCSGG	1 cut(s)	1907
BcoDI	GTCTC	5 cut(s)	457, 676, 1422, 1852, 1924
BcuI	ACTAGT	1 cut(s)	1267
BfaI	CTAG	6 cut(s)	281, 626, 668, 1268, 1814, 1854
BfmI	CTRYAG	1 cut(s)	2364
BfoI	RGCGCY	2 cut(s)	351, 1130
BfuAI	ACCTGC	2 cut(s)	16, 1834
BfuI	GTATCC	2 cut(s)	843, 985
BglI	GCCNNNNNGGC	1 cut(s)	363
BlpI	GCTNAGC	2 cut(s)	543, 1598
Bme1390I	CCNGG	7 cut(s)	136, 377, 616, 764, 941, 1907, 1947
Bme18I	GGWCC	6 cut(s)	682, 759, 902, 937, 1485, 1504
BmeT110I	CYCGRG	1 cut(s)	174
BmrFI	CCNGG	7 cut(s)	136, 377, 616, 764, 941, 1907, 1947
BmsI	GCATC	8 cut(s)	390, 429, 543, 585, 807, 1083, 1726, 2350
BmtI	GCTAGC	1 cut(s)	1857
BpiI	GAAGAC	1 cut(s)	1997
BplI	GAGNNNNNCTC	2 cut(s)	1472, 1504
Bpu1102I	GCTNAGC	2 cut(s)	543, 1598
Bpu14I	TTCGAA	1 cut(s)	1354
BpuEI	CTTGAG	3 cut(s)	353, 533, 971
BpuMI	CCSGG	1 cut(s)	1907
BsaHI	GRCGYC	4 cut(s)	348, 495, 567, 606
BsaI	GGTCTC	1 cut(s)	1852
BsaJI	CCNNGG	9 cut(s)	375, 376, 415, 762, 822, 1122, 1500, 1737, 1946
BsaXI	ACNNNNNCTCC	7 cut(s)	90, 120, 644, 674, 890, 920, 2405
Bsc4I	CCNNNNNNNGG	8 cut(s)	225, 266, 300, 393, 739, 899, 911, 946
Bse118I	RCCGGY	3 cut(s)	19, 647, 698
Bse1I	ACTGG	4 cut(s)	744, 978, 1920, 1938
Bse3DI	GCAATG	1 cut(s)	1698
BseBI	CCWGG	6 cut(s)	136, 377, 616, 764, 941, 1947
BseDI	CCNNGG	9 cut(s)	375, 376, 415, 762, 822, 1122, 1500, 1737, 1946
BseGI	GGATG	6 cut(s)	206, 351, 534, 726, 1386, 1942
BseLI	CCNNNNNNNGG	8 cut(s)	225, 266, 300, 393, 739, 899, 911, 946
BseMI	GCAATG	1 cut(s)	1698
BseMII	CTCAG	4 cut(s)	534, 1612, 1863, 2198
BseNI	ACTGG	4 cut(s)	744, 978, 1920, 1938
BsePI	GCGCGC	1 cut(s)	1671
BseRI	GAGGAG	5 cut(s)	244, 545, 677, 2114, 2171
BseSI	GKGCMC	1 cut(s)	215
BseX3I	CGGCCG	2 cut(s)	571, 2375
BseXI	GCAGC	3 cut(s)	3, 1606, 1614
BseYI	CCCAGC	1 cut(s)	208
BsgI	GTGCAG	1 cut(s)	2298
Bsh1285I	CGRYCG	2 cut(s)	574, 2378
BshNI	GGYRCC	8 cut(s)	4, 347, 425, 695, 1105, 1424, 1733, 1835
BsiEI	CGRYCG	2 cut(s)	574, 2378
BsiHKAI	GWGCWC	6 cut(s)	339, 549, 789, 1167, 1476, 1785
BsiHKCI	CYCGRG	1 cut(s)	174
BsiSI	CCGG	6 cut(s)	20, 166, 648, 699, 1906, 2411
BslFI	GGGAC	5 cut(s)	358, 745, 1090, 1764, 2237
BslI	CCNNNNNNNGG	8 cut(s)	225, 266, 300, 393, 739, 899, 911, 946
BsmAI	GTCTC	5 cut(s)	457, 676, 1422, 1852, 1924
BsmBI	CGTCTC	1 cut(s)	1422
BsmFI	GGGAC	5 cut(s)	358, 745, 1090, 1764, 2237
BsmI	GAATGC	1 cut(s)	221
Bso31I	GGTCTC	1 cut(s)	1852
BsoBI	CYCGRG	1 cut(s)	174
Bsp119I	TTCGAA	1 cut(s)	1354
Bsp120I	GGGCCC	1 cut(s)	211
Bsp1407I	TGTACA	2 cut(s)	1049, 1281
Bsp143I	GATC	7 cut(s)	56, 102, 396, 618, 882, 1068, 2184
Bsp1720I	GCTNAGC	2 cut(s)	543, 1598
Bsp19I	CCATGG	2 cut(s)	1500, 1737
BspCNI	CTCAG	4 cut(s)	535, 1611, 1862, 2199
BspHI	TCATGA	1 cut(s)	744
BspMI	ACCTGC	2 cut(s)	16, 1834
BspOI	GCTAGC	1 cut(s)	1857
BspPI	GGATC	6 cut(s)	64, 97, 404, 613, 626, 890
BspQI	GCTCTTC	1 cut(s)	96
BspT104I	TTCGAA	1 cut(s)	1354
BspT107I	GGYRCC	8 cut(s)	4, 347, 425, 695, 1105, 1424, 1733, 1835
BspTNI	GGTCTC	1 cut(s)	1852
BsrBI	CCGCTC	1 cut(s)	1483
BsrDI	GCAATG	1 cut(s)	1698
BsrFI	RCCGGY	3 cut(s)	19, 647, 698
BsrGI	TGTACA	2 cut(s)	1049, 1281
BsrI	ACTGG	4 cut(s)	744, 978, 1920, 1938
BssAI	RCCGGY	3 cut(s)	19, 647, 698
BssECI	CCNNGG	9 cut(s)	375, 376, 415, 762, 822, 1122, 1500, 1737, 1946
BssHII	GCGCGC	1 cut(s)	1671
BssMI	GATC	7 cut(s)	56, 102, 396, 618, 882, 1068, 2184
BssNAI	GTATAC	1 cut(s)	1869
BssNI	GRCGYC	4 cut(s)	348, 495, 567, 606
BssSI	CACGAG	1 cut(s)	1652
BssT1I	CCWWGG	3 cut(s)	1122, 1500, 1737
Bst1107I	GTATAC	1 cut(s)	1869
Bst2BI	CACGAG	1 cut(s)	1652
Bst2UI	CCWGG	6 cut(s)	136, 377, 616, 764, 941, 1947
Bst4CI	ACNGT	9 cut(s)	277, 289, 451, 1081, 1087, 1687, 1963, 2251, 2276
Bst6I	CTCTTC	8 cut(s)	96, 323, 1541, 1639, 1891, 2073, 2133, 2329
BstACI	GRCGYC	4 cut(s)	348, 495, 567, 606
BstAUI	TGTACA	2 cut(s)	1049, 1281
BstBI	TTCGAA	1 cut(s)	1354
BstDEI	CTNAG	5 cut(s)	543, 1598, 1849, 2207, 2305
BstDSI	CCRYGG	3 cut(s)	822, 1500, 1737
BstF5I	GGATG	6 cut(s)	206, 351, 534, 726, 1386, 1942
BstH2I	RGCGCY	2 cut(s)	351, 1130
BstKTI	GATC	7 cut(s)	59, 105, 399, 621, 885, 1071, 2187
BstMAI	GTCTC	5 cut(s)	457, 676, 1422, 1852, 1924
BstMBI	GATC	7 cut(s)	56, 102, 396, 618, 882, 1068, 2184
BstMCI	CGRYCG	2 cut(s)	574, 2378
BstNI	CCWGG	6 cut(s)	136, 377, 616, 764, 941, 1947
BstNSI	RCATGY	3 cut(s)	1368, 1651, 1793
BstSCI	CCNGG	7 cut(s)	134, 375, 614, 762, 939, 1905, 1945
BstSFI	CTRYAG	1 cut(s)	2364
BstSLI	GKGCMC	1 cut(s)	215
BstV1I	GCAGC	3 cut(s)	3, 1606, 1614
BstV2I	GAAGAC	1 cut(s)	1997
BstX2I	RGATCY	1 cut(s)	618
BstYI	RGATCY	1 cut(s)	618
BstZ17I	GTATAC	1 cut(s)	1869
BstZI	CGGCCG	2 cut(s)	571, 2375
BsuI	GTATCC	2 cut(s)	843, 985
BtgI	CCRYGG	3 cut(s)	822, 1500, 1737
BtgZI	GCGATG	3 cut(s)	367, 562, 622
BtsCI	GGATG	6 cut(s)	206, 351, 534, 726, 1386, 1942
BtsI	GCAGTG	1 cut(s)	1284
BtsIMutI	CAGTG	4 cut(s)	447, 985, 1284, 1913
BveI	ACCTGC	2 cut(s)	16, 1834
CaiI	CAGNNNCTG	1 cut(s)	1726
CciI	TCATGA	1 cut(s)	744
CciNI	GCGGCCGC	1 cut(s)	2375
Cfr10I	RCCGGY	3 cut(s)	19, 647, 698
Cfr42I	CCGCGG	1 cut(s)	825
CseI	GACGC	4 cut(s)	484, 1044, 1594, 2358
CsiI	ACCWGGT	2 cut(s)	134, 939
Csp6I	GTAC	4 cut(s)	1050, 1199, 1282, 2104
CviQI	GTAC	4 cut(s)	1050, 1199, 1282, 2104
DdeI	CTNAG	5 cut(s)	543, 1598, 1849, 2207, 2305
DinI	GGCGCC	1 cut(s)	349
DpnI	GATC	7 cut(s)	58, 104, 398, 620, 884, 1070, 2186
DpnII	GATC	7 cut(s)	56, 102, 396, 618, 882, 1068, 2184
DraI	TTTAAA	1 cut(s)	1641
EaeI	YGGCCR	4 cut(s)	571, 819, 1616, 2375
EagI	CGGCCG	2 cut(s)	571, 2375
Eam1104I	CTCTTC	8 cut(s)	96, 323, 1541, 1639, 1891, 2073, 2133, 2329
EarI	CTCTTC	8 cut(s)	96, 323, 1541, 1639, 1891, 2073, 2133, 2329
EciI	GGCGGA	4 cut(s)	213, 344, 577, 719
Ecl136II	GAGCTC	5 cut(s)	337, 547, 787, 1165, 1474
EclXI	CGGCCG	2 cut(s)	571, 2375
Eco130I	CCWWGG	3 cut(s)	1122, 1500, 1737
Eco147I	AGGCCT	1 cut(s)	1586
Eco24I	GRGCYC	9 cut(s)	215, 306, 339, 549, 789, 987, 1167, 1306, 1476
Eco31I	GGTCTC	1 cut(s)	1852
Eco47I	GGWCC	6 cut(s)	682, 759, 902, 937, 1485, 1504
Eco52I	CGGCCG	2 cut(s)	571, 2375
Eco53kI	GAGCTC	5 cut(s)	337, 547, 787, 1165, 1474
Eco57I	CTGAAG	1 cut(s)	2319
Eco88I	CYCGRG	1 cut(s)	174
EcoICRI	GAGCTC	5 cut(s)	337, 547, 787, 1165, 1474
EcoO109I	RGGNCCY	5 cut(s)	212, 473, 682, 759, 937
EcoRII	CCWGG	6 cut(s)	134, 375, 614, 762, 939, 1945
EcoT14I	CCWWGG	3 cut(s)	1122, 1500, 1737
EcoT38I	GRGCYC	9 cut(s)	215, 306, 339, 549, 789, 987, 1167, 1306, 1476
EgeI	GGCGCC	1 cut(s)	349
EheI	GGCGCC	1 cut(s)	349
ErhI	CCWWGG	3 cut(s)	1122, 1500, 1737
Esp3I	CGTCTC	1 cut(s)	1422
FalI	AAGNNNNNCTT	2 cut(s)	2204, 2236
FaqI	GGGAC	5 cut(s)	358, 745, 1090, 1764, 2237
FauI	CCCGC	8 cut(s)	386, 539, 672, 817, 1004, 1388, 1430, 1523
FauNDI	CATATG	1 cut(s)	1661
FblI	GTMKAC	2 cut(s)	1868, 2246
FokI	GGATG	6 cut(s)	213, 338, 521, 713, 1373, 1929
FriOI	GRGCYC	9 cut(s)	215, 306, 339, 549, 789, 987, 1167, 1306, 1476
FspBI	CTAG	6 cut(s)	281, 626, 668, 1268, 1814, 1854
GsaI	CCCAGC	1 cut(s)	212
HaeII	RGCGCY	2 cut(s)	351, 1130
HapII	CCGG	6 cut(s)	20, 166, 648, 699, 1906, 2411
HgaI	GACGC	4 cut(s)	484, 1044, 1594, 2358
Hin1I	GRCGYC	4 cut(s)	348, 495, 567, 606
HincII	GTYRAC	3 cut(s)	601, 1959, 2247
HindII	GTYRAC	3 cut(s)	601, 1959, 2247
HindIII	AAGCTT	1 cut(s)	2299
HpaII	CCGG	6 cut(s)	20, 166, 648, 699, 1906, 2411
Hpy188I	TCNGA	8 cut(s)	433, 1480, 1722, 1787, 1892, 2208, 2297, 2317
Hpy188III	TCNNGA	6 cut(s)	226, 745, 832, 988, 1075, 1609
HpyCH4III	ACNGT	9 cut(s)	277, 289, 451, 1081, 1087, 1687, 1963, 2251, 2276
HpyCH4IV	ACGT	2 cut(s)	567, 606
HpyCH4V	TGCA	6 cut(s)	146, 798, 1462, 1493, 1651, 2279
HpyF3I	CTNAG	5 cut(s)	543, 1598, 1849, 2207, 2305
HpySE526I	ACGT	2 cut(s)	567, 606
Hsp92I	GRCGYC	4 cut(s)	348, 495, 567, 606
KasI	GGCGCC	1 cut(s)	347
KroI	GCCGGC	2 cut(s)	19, 698
KroNI	GCCGGC	2 cut(s)	21, 700
KspI	CCGCGG	1 cut(s)	825
Kzo9I	GATC	7 cut(s)	56, 102, 396, 618, 882, 1068, 2184
LguI	GCTCTTC	1 cut(s)	96
LmnI	GCTCC	9 cut(s)	231, 558, 784, 1162, 1301, 1471, 1480, 1788, 2006
Lsp1109I	GCAGC	3 cut(s)	3, 1606, 1614
LweI	GCATC	8 cut(s)	390, 429, 543, 585, 807, 1083, 1726, 2350
MabI	ACCWGGT	2 cut(s)	134, 939
MaeI	CTAG	6 cut(s)	281, 626, 668, 1268, 1814, 1854
MaeII	ACGT	2 cut(s)	567, 606
MaeIII	GTNAC	3 cut(s)	832, 1081, 2270
MalI	GATC	7 cut(s)	58, 104, 398, 620, 884, 1070, 2186
MbiI	CCGCTC	1 cut(s)	1483
MboI	GATC	7 cut(s)	56, 102, 396, 618, 882, 1068, 2184
MflI	RGATCY	1 cut(s)	618
MluCI	AATT	2 cut(s)	994, 2012
Mly113I	GGCGCC	1 cut(s)	348
MlyI	GAGTC	9 cut(s)	186, 1421, 1437, 1751, 1821, 2150, 2197, 2273, 2423
MmeI	TCCRAC	1 cut(s)	1810
MroNI	GCCGGC	2 cut(s)	19, 698
MseI	TTAA	2 cut(s)	1640, 2198
MslI	CAYNNNNRTG	2 cut(s)	275, 581
MspA1I	CMGCKG	3 cut(s)	16, 824, 2030
MspI	CCGG	6 cut(s)	20, 166, 648, 699, 1906, 2411
MspR9I	CCNGG	7 cut(s)	136, 377, 616, 764, 941, 1907, 1947
Mva1269I	GAATGC	1 cut(s)	221
MvaI	CCWGG	6 cut(s)	136, 377, 616, 764, 941, 1947
NaeI	GCCGGC	2 cut(s)	21, 700
NarI	GGCGCC	1 cut(s)	348
NciI	CCSGG	1 cut(s)	1907
NcoI	CCATGG	2 cut(s)	1500, 1737
NdeI	CATATG	1 cut(s)	1661
NdeII	GATC	7 cut(s)	56, 102, 396, 618, 882, 1068, 2184
NgoMIV	GCCGGC	2 cut(s)	19, 698
NheI	GCTAGC	1 cut(s)	1853
NmeAIII	GCCGAG	1 cut(s)	396
NmuCI	GTSAC	3 cut(s)	832, 1081, 2270
NotI	GCGGCCGC	1 cut(s)	2375
NspI	RCATGY	3 cut(s)	1368, 1651, 1793
NspV	TTCGAA	1 cut(s)	1354
PaeI	GCATGC	1 cut(s)	1368
PagI	TCATGA	1 cut(s)	744
PaqCI	CACCTGC	1 cut(s)	16
PasI	CCCWGGG	1 cut(s)	376
PauI	GCGCGC	1 cut(s)	1671
PceI	AGGCCT	1 cut(s)	1586
PciSI	GCTCTTC	1 cut(s)	96
PcsI	WCGNNNNNNNCGW	5 cut(s)	429, 714, 870, 1650, 2252
PctI	GAATGC	1 cut(s)	221
PdiI	GCCGGC	2 cut(s)	21, 700
PfeI	GAWTC	4 cut(s)	1612, 1810, 1971, 2347
PleI	GAGTC	9 cut(s)	185, 1420, 1437, 1750, 1821, 2149, 2197, 2272, 2422
PluTI	GGCGCC	1 cut(s)	351
PpsI	GAGTC	9 cut(s)	185, 1420, 1437, 1750, 1821, 2149, 2197, 2272, 2422
PpuMI	RGGWCCY	3 cut(s)	682, 759, 937
Psp124BI	GAGCTC	5 cut(s)	339, 549, 789, 1167, 1476
Psp5II	RGGWCCY	3 cut(s)	682, 759, 937
Psp6I	CCWGG	6 cut(s)	134, 375, 614, 762, 939, 1945
PspFI	CCCAGC	1 cut(s)	208
PspGI	CCWGG	6 cut(s)	134, 375, 614, 762, 939, 1945
PspOMI	GGGCCC	1 cut(s)	211
PspPPI	RGGWCCY	3 cut(s)	682, 759, 937
PstNI	CAGNNNCTG	1 cut(s)	1726
PsuI	RGATCY	1 cut(s)	618
PteI	GCGCGC	1 cut(s)	1671
PvuII	CAGCTG	1 cut(s)	2030
RsaI	GTAC	4 cut(s)	1051, 1200, 1283, 2105
RsaNI	GTAC	4 cut(s)	1050, 1199, 1282, 2104
RseI	CAYNNNNRTG	2 cut(s)	275, 581
SacI	GAGCTC	5 cut(s)	339, 549, 789, 1167, 1476
SacII	CCGCGG	1 cut(s)	825
SalI	GTCGAC	1 cut(s)	2245
SapI	GCTCTTC	1 cut(s)	96
SaqAI	TTAA	2 cut(s)	1640, 2198
Sau3AI	GATC	7 cut(s)	56, 102, 396, 618, 882, 1068, 2184
SchI	GAGTC	9 cut(s)	186, 1421, 1437, 1751, 1821, 2150, 2197, 2273, 2423
ScrFI	CCNGG	7 cut(s)	136, 377, 616, 764, 941, 1907, 1947
SexAI	ACCWGGT	2 cut(s)	134, 939
SfaNI	GCATC	8 cut(s)	390, 429, 543, 585, 807, 1083, 1726, 2350
SfcI	CTRYAG	1 cut(s)	2364
SfoI	GGCGCC	1 cut(s)	349
Sfr303I	CCGCGG	1 cut(s)	825
SfuI	TTCGAA	1 cut(s)	1354
SgrBI	CCGCGG	1 cut(s)	825
SinI	GGWCC	6 cut(s)	682, 759, 902, 937, 1485, 1504
SmiMI	CAYNNNNRTG	2 cut(s)	275, 581
SmlI	CTYRAG	3 cut(s)	332, 548, 986
SmoI	CTYRAG	3 cut(s)	332, 548, 986
SpeI	ACTAGT	1 cut(s)	1267
SphI	GCATGC	1 cut(s)	1368
Sse9I	AATT	2 cut(s)	994, 2012
SseBI	AGGCCT	1 cut(s)	1586
SspDI	GGCGCC	1 cut(s)	347
SspI	AATATT	2 cut(s)	1180, 1359
SspMI	CTAG	6 cut(s)	281, 626, 668, 1268, 1814, 1854
SstI	GAGCTC	5 cut(s)	339, 549, 789, 1167, 1476
StuI	AGGCCT	1 cut(s)	1586
StyD4I	CCNGG	7 cut(s)	134, 375, 614, 762, 939, 1905, 1945
StyI	CCWWGG	3 cut(s)	1122, 1500, 1737
TaaI	ACNGT	9 cut(s)	277, 289, 451, 1081, 1087, 1687, 1963, 2251, 2276
TaiI	ACGT	2 cut(s)	570, 609
TaqI	TCGA	6 cut(s)	339, 1091, 1354, 1410, 2246, 2267
TasI	AATT	2 cut(s)	994, 2012
TatI	WGTACW	3 cut(s)	1049, 1281, 2103
TfiI	GAWTC	4 cut(s)	1612, 1810, 1971, 2347
Tru1I	TTAA	2 cut(s)	1640, 2198
Tru9I	TTAA	2 cut(s)	1640, 2198
TscAI	CASTG	4 cut(s)	454, 985, 1284, 1920
TseFI	GTSAC	3 cut(s)	832, 1081, 2270
TseI	GCWGC	3 cut(s)	16, 1594, 1602
Tsp45I	GTSAC	3 cut(s)	832, 1081, 2270
TspDTI	ATGAA	4 cut(s)	53, 1157, 1243, 2091
TspGWI	ACGGA	3 cut(s)	664, 851, 894
TspRI	CASTG	4 cut(s)	454, 985, 1284, 1920
VpaK11BI	GGWCC	6 cut(s)	682, 759, 902, 937, 1485, 1504
XceI	RCATGY	3 cut(s)	1368, 1651, 1793
XcmI	CCANNNNNNNNNTGG	2 cut(s)	1465, 1744
XmiI	GTMKAC	2 cut(s)	1868, 2246
XspI	CTAG	6 cut(s)	281, 626, 668, 1268, 1814, 1854
ZraI	GACGTC	2 cut(s)	568, 607

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.