Basic Information

Type: gene

Biological Identity

Species

rosa_roxburghii

Chromosome

GWHEROQ00000006

Orthogroup ID

OG0004570

Physical Location & Seq

Genomic Coordinates Forward (+)

19936669 .. 19939514

Gene Structure 2846 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

Rroxscaffold_6G00398090.1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1995 bp

ATGACGGTGCTAGACACGGTAGCCAACATCGGGCTCTGCTTCTTCCTCTTCCTCGTCGGCCTTGAGCTCGACATCGGCGCCATCCGCCGCACAGGCAAGAAGTCCCTCGGCATCGCCTTCTGCGGGATCACCCTCCCCTTCGTCCTCGGCATTGGCACCTCCGAAGCCCTACGCGCCACCGTCTCGAAAGGCGTGAAACAAGGCCCCTTCCTCGTTTTCAACGGCGTCGCCCTCTCCATCACCGCCTTCCCAGTCCTTGCCCGCATCCTCGCTGAGCTCAAGCTCCTCACCACCGACGTCGGCCGCATCGCCATGTCCGCTGCCGCCGTCAACGACGTCGCCGCCTGGATCCTTCTAGCCCTCGCCATCGCCCTCACCGGCAACAACACCTCCCCGCTCGTCTCCGTCTGGGTCCTCCTCTGCGGTGCCGGCTTCGTCGGGTTCGCCATCGTCGCCATCCGCCCCATACTGGTCATGATGGCGCGAAGGTCCCAGGAGGGCGAACCCGTGAAGGAGCTCTACATCTGCATCACTCTCTCACTGGTTTTGGCCGCGGGGTTCGTGACGGACACGATTGGGATACACGCGCTGTTCGGCGCGTTCGTGGTGGGGATCATAATGCCGAAGGAGGGTCCGTTCGCGGCATCGTTTGGGAAGATCGTCGGGACTGTCACCGTGTCGATGCTGTTCAAGGTGCCTTTCAGGGAAGCCTTGGCGCTTGGCTTCCTTATGAATACAAAGGGCTTGGTGGAGCTCATCGTACTGAACGAACAGACTTTTGCCATCTTTATTTTGATGGCGTTGTTCACCACCTTCATCACCACACCACTAGTGATGGCAGTGTACAAACCAGCAAGAAAGGGAGCCCCTTACAAACTACGCACCATTTTCCGCAAAAGCCCAGACACAGAACTTCGAATATTGGCATGCTTCCACACCACCCGCAACATCCCCACCATGATCAACCTCATCGAGTCATCTCGCGGCACCCACAAGCGCGGACGACTCACCGTCTACGCCATGCACCTCATGGAGCTCTCGGAGCGGTCCTCTGCGATCTCCATGGTCCACAAAGCCCGCTATAATGGCCTCCCATTTTGGAACAAGAAGAGTGATGGAAATGACCAAATGGTTATTGCTTTCGAGGCCTACGAGCAGCTCAGCAGCGTCAAGATTCGGCCAGCCACCGCCATCTCTTCTTTAAACGACATGCACGAGGACATATGCGCCTGCGCGCACCAAAAAACCGTGGCAATGATTTTGTTGCCGTTTCACAAGCATCAGAAGCTGGACGGCACCATGGAGTCGGTGGGACACTCATTTCAAGTGGTGAATGAGCGTGTGCTCCGACATGCGCCTTGTTCGGTTGGGATTCTAGTTGATAGAGGACTCGGTGGCACCACGCAGGTCTCAGCCAGCGACGTGTCGTATACGGCGGTGGTGGCTTTCTTCGGAGGAAGAGATGACCGGGAGGCACTGGCTTATGGTATGAGACTGGCTGAACATCCTGGGATTGCGTTGACTGTTTTTAGATTCGCTGCTCCACAAGGGAAGACATTGAGATTTGGAGCGAAATTAGTTGGGATAACAGCTGACAACAAGAAAATACTGAAAGAAGAAGATAGTAGCCAAGAGAATGAAGAGGACGAGAAGTTCTTGGAGGAGTACAAGAATGTAAGAAGTAGCAGTAAGGAAAAGGAAGAGTCCATGTTTTACGAGGAGAAGGTGGTGGACAACAAAGGGGAGATCGCGGTGGCGTTAAAGACTCTGAGCAAGCACAACTTGTTTATAGTAGGGAGAATGTCGCCGACTGTCCCGTTGGTAGAGTCGAGTGACTGTGCGGAGCTTGGGCCAATCGGAAGCTTCTTAGCCTCGTCAGACTTCTCTTCCACAGCTTCAGTTGTGGTGATTCAACAGTATGATGCTACAGAGTCGCGGCCGCTTGTGGTGGAGGAAGCTGACTATGAGTTGCCGGAGTCGTCGTCTATGGTTTAA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005768 GO:0005770 GO:0006810 GO:0006811 GO:0006812 GO:0006885 GO:0008150 GO:0012505 GO:0015672 GO:0031410 GO:0031982 GO:0042592 GO:0043226 GO:0043227 GO:0043229 GO:0044424 GO:0044444 GO:0044464 GO:0048878 GO:0050801 GO:0051179 GO:0051234 GO:0055067 GO:0055080 GO:0065007 GO:0065008 GO:0097708 GO:0098771

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

Na_H_Exchanger

Protein Analysis

664

Amino Acids

72.18

Weight (kDa)

7.6

Isoelectric Point (pI)

41.0

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Na_H_Exchanger	PF00999	3 - 212	6.7e-31	Sodium/hydrogen exchanger, transmembrane
CHX17_2nd	PF23256	333 - 473	2.2e-51	Plant cation/H(+) antiporter domain
CHX17_C	PF23259	479 - 641	3.6e-18	Plant cation/H(+) antiporter, C-terminal domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

Style Settings

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0004570)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT3G17630 AT3G17630
fragaria_vesca	FvH4_4g21880
malus_domestica	MD13G1136000.v1.1 MD16G1131400.v1.1
prunus_persica	Prupe.1G209400_v2.0.a1
pyrus_communis	pycom16g11580
rosa_chinensis	RchiOBHm_Chr3g0483861
rosa_laevigata	RLG00000007178 RLG00000023257
rosa_multiflora	Rmu_co8183874.1_g000001 Rmu_co8462775.1_g000001 Rmu_sc0004940.1_g000012 Rmu_sc0005901.1_g000001 Rmu_sc0009599.1_g000012
rosa_roxburghii	Rroxscaffold_5G00369850 Rroxscaffold_6G00398090
rosa_rugosa	Rorug03G0208200.1 Rorug03G0208300 Rorug04G0222700 Rorug04G0222800
rosa_samantha	Rh3AG257000 Rh3BG293400 Rh3CG291700 Rh3DG286700 Rh4AG279900 Rh4AG280100 Rh4BG285300 Rh4BG285500 Rh4CG300900
rosa_wichuraiana	Rw3G023230

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AatII	GACGTC	2 cut(s)	300, 339
Acc36I	ACCTGC	1 cut(s)	1396
AccB1I	GGYRCC	7 cut(s)	77, 155, 425, 694, 986, 1295, 1397
AccBSI	CCGCTC	2 cut(s)	397, 1045
AccI	GTMKAC	2 cut(s)	1014, 1430
AclWI	GGATC	4 cut(s)	134, 343, 356, 620
AcoI	YGGCCR	4 cut(s)	301, 549, 1178, 1937
AcuI	CTGAAG	1 cut(s)	1881
AcyI	GRCGYC	4 cut(s)	78, 225, 297, 336
AfaI	GTAC	3 cut(s)	762, 845, 1667
AfiI	CCNNNNNNNGG	4 cut(s)	30, 123, 469, 629
AflIII	ACRYGT	1 cut(s)	1422
AgsI	TTSAA	4 cut(s)	220, 691, 1325, 1913
AhdI	GACNNNNNGTC	1 cut(s)	1010
AhlI	ACTAGT	1 cut(s)	829
AjiI	CACGTC	1 cut(s)	1423
AjnI	CCWGG	3 cut(s)	344, 492, 1507
AloI	GAACNNNNNNTCC	4 cut(s)	620, 652, 1637, 1669
Alw21I	GWGCWC	6 cut(s)	69, 279, 519, 756, 1038, 1347
Alw26I	GTCTC	4 cut(s)	187, 406, 1414, 1486
AlwI	GGATC	4 cut(s)	134, 343, 356, 620
AlwNI	CAGNNNCTG	1 cut(s)	1288
AoxI	GGCC	9 cut(s)	58, 202, 301, 549, 1087, 1146, 1178, 1850, 1937
ApeKI	GCWGC	4 cut(s)	320, 1156, 1164, 1538
AspS9I	GGNCC	7 cut(s)	203, 412, 489, 632, 1047, 1066, 1850
AsuC2I	CCSGG	1 cut(s)	1469
AsuII	TTCGAA	1 cut(s)	916
AvaII	GGWCC	5 cut(s)	412, 489, 632, 1047, 1066
BamHI	GGATCC	1 cut(s)	348
BanI	GGYRCC	7 cut(s)	77, 155, 425, 694, 986, 1295, 1397
BanII	GRGCYC	7 cut(s)	36, 69, 279, 519, 756, 868, 1038
BarI	GAAGNNNNNNTAC	2 cut(s)	503, 535
BauI	CACGAG	1 cut(s)	1214
BbsI	GAAGAC	1 cut(s)	1559
Bbv12I	GWGCWC	6 cut(s)	69, 279, 519, 756, 1038, 1347
BbvI	GCAGC	4 cut(s)	307, 1168, 1176, 1525
BceAI	ACGGC	5 cut(s)	238, 311, 1252, 1309, 1449
BciT130I	CCWGG	3 cut(s)	346, 494, 1509
BciVI	GTATCC	1 cut(s)	573
BclI	TGATCA	1 cut(s)	960
BcnI	CCSGG	1 cut(s)	1469
BcoDI	GTCTC	4 cut(s)	187, 406, 1414, 1486
BcuI	ACTAGT	1 cut(s)	829
BfaI	CTAG	4 cut(s)	11, 356, 830, 1376
BfmI	CTRYAG	1 cut(s)	1926
BfoI	RGCGCY	2 cut(s)	81, 719
BfuAI	ACCTGC	1 cut(s)	1396
BfuI	GTATCC	1 cut(s)	573
BglI	GCCNNNNNGGC	1 cut(s)	93
BlpI	GCTNAGC	2 cut(s)	273, 1160
Bme1390I	CCNGG	4 cut(s)	346, 494, 1469, 1509
Bme18I	GGWCC	5 cut(s)	412, 489, 632, 1047, 1066
BmeRI	GACNNNNNGTC	1 cut(s)	1010
BmgBI	CACGTC	1 cut(s)	1423
BmgT120I	GGNCC	7 cut(s)	203, 412, 489, 632, 1047, 1066, 1850
BmrFI	CCNGG	4 cut(s)	346, 494, 1469, 1509
BmrI	ACTGGG	1 cut(s)	245
BmsI	GCATC	8 cut(s)	120, 273, 315, 537, 653, 672, 1288, 1912
BmuI	ACTGGG	1 cut(s)	245
BpiI	GAAGAC	1 cut(s)	1559
BplI	GAGNNNNNCTC	2 cut(s)	1034, 1066
Bpu1102I	GCTNAGC	2 cut(s)	273, 1160
Bpu14I	TTCGAA	1 cut(s)	916
BpuEI	CTTGAG	2 cut(s)	83, 263
BpuMI	CCSGG	1 cut(s)	1469
BsaHI	GRCGYC	4 cut(s)	78, 225, 297, 336
BsaI	GGTCTC	1 cut(s)	1414
BsaJI	CCNNGG	9 cut(s)	106, 145, 492, 552, 711, 1062, 1248, 1299, 1508
BsaXI	ACNNNNNCTCC	5 cut(s)	374, 404, 620, 650, 1967
Bsc4I	CCNNNNNNNGG	4 cut(s)	30, 123, 469, 629
Bse118I	RCCGGY	2 cut(s)	377, 428
Bse1I	ACTGG	5 cut(s)	251, 474, 546, 1482, 1500
Bse3DI	GCAATG	1 cut(s)	1260
BseBI	CCWGG	3 cut(s)	346, 494, 1509
BseDI	CCNNGG	9 cut(s)	106, 145, 492, 552, 711, 1062, 1248, 1299, 1508
BseGI	GGATG	5 cut(s)	81, 264, 456, 948, 1504
BseLI	CCNNNNNNNGG	4 cut(s)	30, 123, 469, 629
BseMI	GCAATG	1 cut(s)	1260
BseMII	CTCAG	4 cut(s)	264, 1174, 1425, 1760
BseNI	ACTGG	5 cut(s)	251, 474, 546, 1482, 1500
BsePI	GCGCGC	1 cut(s)	1233
BseRI	GAGGAG	4 cut(s)	275, 407, 1676, 1733
BseX3I	CGGCCG	2 cut(s)	301, 1937
BseXI	GCAGC	4 cut(s)	307, 1168, 1176, 1525
Bsh1285I	CGRYCG	2 cut(s)	304, 1940
BshFI	GGCC	9 cut(s)	60, 204, 303, 551, 1089, 1148, 1180, 1852, 1939
BshNI	GGYRCC	7 cut(s)	77, 155, 425, 694, 986, 1295, 1397
BsiEI	CGRYCG	2 cut(s)	304, 1940
BsiHKAI	GWGCWC	6 cut(s)	69, 279, 519, 756, 1038, 1347
BsiSI	CCGG	4 cut(s)	378, 429, 1468, 1973
BslFI	GGGAC	5 cut(s)	88, 475, 679, 1326, 1799
BslI	CCNNNNNNNGG	4 cut(s)	30, 123, 469, 629
BsmAI	GTCTC	4 cut(s)	187, 406, 1414, 1486
BsmBI	CGTCTC	2 cut(s)	187, 406
BsmFI	GGGAC	5 cut(s)	88, 475, 679, 1326, 1799
BsnI	GGCC	9 cut(s)	60, 204, 303, 551, 1089, 1148, 1180, 1852, 1939
Bso31I	GGTCTC	1 cut(s)	1414
Bsp119I	TTCGAA	1 cut(s)	916
Bsp1286I	GDGCHC	8 cut(s)	36, 69, 279, 519, 756, 868, 1038, 1347
Bsp1407I	TGTACA	1 cut(s)	843
Bsp143I	GATC	7 cut(s)	126, 348, 612, 657, 960, 1056, 1746
Bsp1720I	GCTNAGC	2 cut(s)	273, 1160
Bsp19I	CCATGG	2 cut(s)	1062, 1299
BspANI	GGCC	9 cut(s)	60, 204, 303, 551, 1089, 1148, 1180, 1852, 1939
BspCNI	CTCAG	4 cut(s)	265, 1173, 1424, 1761
BspHI	TCATGA	1 cut(s)	474
BspMI	ACCTGC	1 cut(s)	1396
BspPI	GGATC	4 cut(s)	134, 343, 356, 620
BspT104I	TTCGAA	1 cut(s)	916
BspT107I	GGYRCC	7 cut(s)	77, 155, 425, 694, 986, 1295, 1397
BspTNI	GGTCTC	1 cut(s)	1414
BsrBI	CCGCTC	2 cut(s)	397, 1045
BsrDI	GCAATG	1 cut(s)	1260
BsrFI	RCCGGY	2 cut(s)	377, 428
BsrGI	TGTACA	1 cut(s)	843
BsrI	ACTGG	5 cut(s)	251, 474, 546, 1482, 1500
BssAI	RCCGGY	2 cut(s)	377, 428
BssECI	CCNNGG	9 cut(s)	106, 145, 492, 552, 711, 1062, 1248, 1299, 1508
BssHII	GCGCGC	1 cut(s)	1233
BssMI	GATC	7 cut(s)	126, 348, 612, 657, 960, 1056, 1746
BssNAI	GTATAC	1 cut(s)	1431
BssNI	GRCGYC	4 cut(s)	78, 225, 297, 336
BssSI	CACGAG	1 cut(s)	1214
BssT1I	CCWWGG	3 cut(s)	711, 1062, 1299
Bst1107I	GTATAC	1 cut(s)	1431
Bst2BI	CACGAG	1 cut(s)	1214
Bst2UI	CCWGG	3 cut(s)	346, 494, 1509
Bst6I	CTCTTC	7 cut(s)	53, 1103, 1201, 1453, 1635, 1695, 1891
BstACI	GRCGYC	4 cut(s)	78, 225, 297, 336
BstAUI	TGTACA	1 cut(s)	843
BstBI	TTCGAA	1 cut(s)	916
BstC8I	GCNNGC	9 cut(s)	262, 430, 928, 1078, 1182, 1231, 1235, 1417, 1775
BstDEI	CTNAG	5 cut(s)	273, 1160, 1411, 1769, 1867
BstDSI	CCRYGG	4 cut(s)	552, 1062, 1248, 1299
BstF5I	GGATG	5 cut(s)	81, 264, 456, 948, 1504
BstH2I	RGCGCY	2 cut(s)	81, 719
BstKTI	GATC	7 cut(s)	129, 351, 615, 660, 963, 1059, 1749
BstMAI	GTCTC	4 cut(s)	187, 406, 1414, 1486
BstMBI	GATC	7 cut(s)	126, 348, 612, 657, 960, 1056, 1746
BstMCI	CGRYCG	2 cut(s)	304, 1940
BstNI	CCWGG	3 cut(s)	346, 494, 1509
BstNSI	RCATGY	3 cut(s)	930, 1213, 1355
BstSCI	CCNGG	4 cut(s)	344, 492, 1467, 1507
BstSFI	CTRYAG	1 cut(s)	1926
BstV1I	GCAGC	4 cut(s)	307, 1168, 1176, 1525
BstV2I	GAAGAC	1 cut(s)	1559
BstX2I	RGATCY	1 cut(s)	348
BstYI	RGATCY	1 cut(s)	348
BstZ17I	GTATAC	1 cut(s)	1431
BstZI	CGGCCG	2 cut(s)	301, 1937
BsuI	GTATCC	1 cut(s)	573
BsuRI	GGCC	9 cut(s)	60, 204, 303, 551, 1089, 1148, 1180, 1852, 1939
BtgI	CCRYGG	4 cut(s)	552, 1062, 1248, 1299
BtgZI	GCGATG	3 cut(s)	97, 292, 352
BtrI	CACGTC	1 cut(s)	1423
BtsCI	GGATG	5 cut(s)	81, 264, 456, 948, 1504
BtsI	GCAGTG	1 cut(s)	846
BtsIMutI	CAGTG	3 cut(s)	539, 846, 1475
BveI	ACCTGC	1 cut(s)	1396
Cac8I	GCNNGC	9 cut(s)	262, 430, 928, 1078, 1182, 1231, 1235, 1417, 1775
CaiI	CAGNNNCTG	1 cut(s)	1288
CciI	TCATGA	1 cut(s)	474
CciNI	GCGGCCGC	1 cut(s)	1937
Cfr10I	RCCGGY	2 cut(s)	377, 428
Cfr13I	GGNCC	7 cut(s)	203, 412, 489, 632, 1047, 1066, 1850
Cfr42I	CCGCGG	1 cut(s)	555
CseI	GACGC	2 cut(s)	214, 1156
Csp6I	GTAC	3 cut(s)	761, 844, 1666
CviQI	GTAC	3 cut(s)	761, 844, 1666
DdeI	CTNAG	5 cut(s)	273, 1160, 1411, 1769, 1867
DinI	GGCGCC	1 cut(s)	79
DpnI	GATC	7 cut(s)	128, 350, 614, 659, 962, 1058, 1748
DpnII	GATC	7 cut(s)	126, 348, 612, 657, 960, 1056, 1746
DraI	TTTAAA	1 cut(s)	1203
DriI	GACNNNNNGTC	1 cut(s)	1010
EaeI	YGGCCR	4 cut(s)	301, 549, 1178, 1937
EagI	CGGCCG	2 cut(s)	301, 1937
Eam1104I	CTCTTC	7 cut(s)	53, 1103, 1201, 1453, 1635, 1695, 1891
Eam1105I	GACNNNNNGTC	1 cut(s)	1010
EarI	CTCTTC	7 cut(s)	53, 1103, 1201, 1453, 1635, 1695, 1891
EciI	GGCGGA	2 cut(s)	74, 449
Ecl136II	GAGCTC	5 cut(s)	67, 277, 517, 754, 1036
EclXI	CGGCCG	2 cut(s)	301, 1937
Eco130I	CCWWGG	3 cut(s)	711, 1062, 1299
Eco147I	AGGCCT	1 cut(s)	1148
Eco24I	GRGCYC	7 cut(s)	36, 69, 279, 519, 756, 868, 1038
Eco31I	GGTCTC	1 cut(s)	1414
Eco47I	GGWCC	5 cut(s)	412, 489, 632, 1047, 1066
Eco52I	CGGCCG	2 cut(s)	301, 1937
Eco53kI	GAGCTC	5 cut(s)	67, 277, 517, 754, 1036
Eco57I	CTGAAG	1 cut(s)	1881
EcoICRI	GAGCTC	5 cut(s)	67, 277, 517, 754, 1036
EcoO109I	RGGNCCY	3 cut(s)	203, 412, 489
EcoRII	CCWGG	3 cut(s)	344, 492, 1507
EcoT14I	CCWWGG	3 cut(s)	711, 1062, 1299
EcoT38I	GRGCYC	7 cut(s)	36, 69, 279, 519, 756, 868, 1038
EgeI	GGCGCC	1 cut(s)	79
EheI	GGCGCC	1 cut(s)	79
ErhI	CCWWGG	3 cut(s)	711, 1062, 1299
Esp3I	CGTCTC	2 cut(s)	187, 406
FalI	AAGNNNNNCTT	2 cut(s)	1766, 1798
FaqI	GGGAC	5 cut(s)	88, 475, 679, 1326, 1799
FauI	CCCGC	6 cut(s)	116, 269, 402, 547, 950, 1085
FauNDI	CATATG	1 cut(s)	1223
FbaI	TGATCA	1 cut(s)	960
FblI	GTMKAC	2 cut(s)	1014, 1430
FokI	GGATG	5 cut(s)	68, 251, 443, 935, 1491
FriOI	GRGCYC	7 cut(s)	36, 69, 279, 519, 756, 868, 1038
FspBI	CTAG	4 cut(s)	11, 356, 830, 1376
HaeII	RGCGCY	2 cut(s)	81, 719
HaeIII	GGCC	9 cut(s)	60, 204, 303, 551, 1089, 1148, 1180, 1852, 1939
HapII	CCGG	4 cut(s)	378, 429, 1468, 1973
HgaI	GACGC	2 cut(s)	214, 1156
Hin1I	GRCGYC	4 cut(s)	78, 225, 297, 336
HincII	GTYRAC	2 cut(s)	331, 1521
HindII	GTYRAC	2 cut(s)	331, 1521
HindIII	AAGCTT	1 cut(s)	1861
HpaII	CCGG	4 cut(s)	378, 429, 1468, 1973
Hpy166II	GTNNAC	8 cut(s)	331, 807, 844, 1015, 1069, 1431, 1521, 1732
Hpy188I	TCNGA	8 cut(s)	163, 1042, 1284, 1349, 1454, 1770, 1859, 1879
Hpy188III	TCNNGA	5 cut(s)	184, 475, 562, 664, 1171
Hpy8I	GTNNAC	8 cut(s)	331, 807, 844, 1015, 1069, 1431, 1521, 1732
HpyCH4IV	ACGT	3 cut(s)	297, 336, 1422
HpyCH4V	TGCA	3 cut(s)	528, 1024, 1213
HpyF3I	CTNAG	5 cut(s)	273, 1160, 1411, 1769, 1867
HpySE526I	ACGT	3 cut(s)	297, 336, 1422
Hsp92I	GRCGYC	4 cut(s)	78, 225, 297, 336
KasI	GGCGCC	1 cut(s)	77
KroI	GCCGGC	1 cut(s)	428
KroNI	GCCGGC	1 cut(s)	430
Ksp22I	TGATCA	1 cut(s)	960
KspI	CCGCGG	1 cut(s)	555
Kzo9I	GATC	7 cut(s)	126, 348, 612, 657, 960, 1056, 1746
Lsp1109I	GCAGC	4 cut(s)	307, 1168, 1176, 1525
LweI	GCATC	8 cut(s)	120, 273, 315, 537, 653, 672, 1288, 1912
MaeI	CTAG	4 cut(s)	11, 356, 830, 1376
MaeII	ACGT	3 cut(s)	297, 336, 1422
MaeIII	GTNAC	3 cut(s)	562, 670, 1832
MalI	GATC	7 cut(s)	128, 350, 614, 659, 962, 1058, 1748
MbiI	CCGCTC	2 cut(s)	397, 1045
MboI	GATC	7 cut(s)	126, 348, 612, 657, 960, 1056, 1746
MflI	RGATCY	1 cut(s)	348
MhlI	GDGCHC	8 cut(s)	36, 69, 279, 519, 756, 868, 1038, 1347
MluCI	AATT	1 cut(s)	1574
Mly113I	GGCGCC	1 cut(s)	78
MlyI	GAGTC	9 cut(s)	983, 999, 1313, 1383, 1712, 1759, 1835, 1940, 1985
MmeI	TCCRAC	1 cut(s)	1372
MroNI	GCCGGC	1 cut(s)	428
MseI	TTAA	3 cut(s)	1202, 1760, 1993
MslI	CAYNNNNRTG	1 cut(s)	311
MspA1I	CMGCKG	3 cut(s)	320, 554, 1592
MspI	CCGG	4 cut(s)	378, 429, 1468, 1973
MspR9I	CCNGG	4 cut(s)	346, 494, 1469, 1509
MvaI	CCWGG	3 cut(s)	346, 494, 1509
NaeI	GCCGGC	1 cut(s)	430
NarI	GGCGCC	1 cut(s)	78
NciI	CCSGG	1 cut(s)	1469
NcoI	CCATGG	2 cut(s)	1062, 1299
NdeI	CATATG	1 cut(s)	1223
NdeII	GATC	7 cut(s)	126, 348, 612, 657, 960, 1056, 1746
NgoMIV	GCCGGC	1 cut(s)	428
NmeAIII	GCCGAG	2 cut(s)	87, 126
NmuCI	GTSAC	3 cut(s)	562, 670, 1832
NotI	GCGGCCGC	1 cut(s)	1937
NspI	RCATGY	3 cut(s)	930, 1213, 1355
NspV	TTCGAA	1 cut(s)	916
PaeI	GCATGC	1 cut(s)	930
PagI	TCATGA	1 cut(s)	474
PauI	GCGCGC	1 cut(s)	1233
PceI	AGGCCT	1 cut(s)	1148
PcsI	WCGNNNNNNNCGW	4 cut(s)	600, 765, 1149, 1212
PdiI	GCCGGC	1 cut(s)	430
PfeI	GAWTC	4 cut(s)	1174, 1372, 1533, 1909
PleI	GAGTC	9 cut(s)	982, 999, 1312, 1383, 1711, 1759, 1834, 1939, 1984
PluTI	GGCGCC	1 cut(s)	81
PpsI	GAGTC	9 cut(s)	982, 999, 1312, 1383, 1711, 1759, 1834, 1939, 1984
PpuMI	RGGWCCY	2 cut(s)	412, 489
Psp124BI	GAGCTC	5 cut(s)	69, 279, 519, 756, 1038
Psp5II	RGGWCCY	2 cut(s)	412, 489
Psp6I	CCWGG	3 cut(s)	344, 492, 1507
PspGI	CCWGG	3 cut(s)	344, 492, 1507
PspPI	GGNCC	7 cut(s)	203, 412, 489, 632, 1047, 1066, 1850
PspPPI	RGGWCCY	2 cut(s)	412, 489
PstNI	CAGNNNCTG	1 cut(s)	1288
PsuI	RGATCY	1 cut(s)	348
PteI	GCGCGC	1 cut(s)	1233
PvuII	CAGCTG	1 cut(s)	1592
RsaI	GTAC	3 cut(s)	762, 845, 1667
RsaNI	GTAC	3 cut(s)	761, 844, 1666
RseI	CAYNNNNRTG	1 cut(s)	311
SacI	GAGCTC	5 cut(s)	69, 279, 519, 756, 1038
SacII	CCGCGG	1 cut(s)	555
SaqAI	TTAA	3 cut(s)	1202, 1760, 1993
Sau3AI	GATC	7 cut(s)	126, 348, 612, 657, 960, 1056, 1746
Sau96I	GGNCC	7 cut(s)	203, 412, 489, 632, 1047, 1066, 1850
SchI	GAGTC	9 cut(s)	983, 999, 1313, 1383, 1712, 1759, 1835, 1940, 1985
ScrFI	CCNGG	4 cut(s)	346, 494, 1469, 1509
SduI	GDGCHC	8 cut(s)	36, 69, 279, 519, 756, 868, 1038, 1347
SfaNI	GCATC	8 cut(s)	120, 273, 315, 537, 653, 672, 1288, 1912
SfcI	CTRYAG	1 cut(s)	1926
SfoI	GGCGCC	1 cut(s)	79
Sfr303I	CCGCGG	1 cut(s)	555
SfuI	TTCGAA	1 cut(s)	916
SgrBI	CCGCGG	1 cut(s)	555
SinI	GGWCC	5 cut(s)	412, 489, 632, 1047, 1066
SmiMI	CAYNNNNRTG	1 cut(s)	311
SmlI	CTYRAG	2 cut(s)	62, 278
SmoI	CTYRAG	2 cut(s)	62, 278
SpeI	ACTAGT	1 cut(s)	829
SphI	GCATGC	1 cut(s)	930
Sse9I	AATT	1 cut(s)	1574
SseBI	AGGCCT	1 cut(s)	1148
SspDI	GGCGCC	1 cut(s)	77
SspI	AATATT	1 cut(s)	921
SspMI	CTAG	4 cut(s)	11, 356, 830, 1376
SstI	GAGCTC	5 cut(s)	69, 279, 519, 756, 1038
StuI	AGGCCT	1 cut(s)	1148
StyD4I	CCNGG	4 cut(s)	344, 492, 1467, 1507
StyI	CCWWGG	3 cut(s)	711, 1062, 1299
TaiI	ACGT	3 cut(s)	300, 339, 1425
TaqI	TCGA	7 cut(s)	69, 185, 680, 916, 972, 1143, 1829
TasI	AATT	1 cut(s)	1574
TatI	WGTACW	2 cut(s)	843, 1665
TauI	GCSGC	9 cut(s)	90, 306, 326, 344, 554, 644, 987, 1939, 1942
TfiI	GAWTC	4 cut(s)	1174, 1372, 1533, 1909
Tru1I	TTAA	3 cut(s)	1202, 1760, 1993
Tru9I	TTAA	3 cut(s)	1202, 1760, 1993
TscAI	CASTG	3 cut(s)	546, 846, 1482
TseFI	GTSAC	3 cut(s)	562, 670, 1832
TseI	GCWGC	4 cut(s)	320, 1156, 1164, 1538
Tsp45I	GTSAC	3 cut(s)	562, 670, 1832
TspDTI	ATGAA	3 cut(s)	746, 805, 1653
TspGWI	ACGGA	3 cut(s)	394, 581, 624
TspRI	CASTG	3 cut(s)	546, 846, 1482
VpaK11BI	GGWCC	5 cut(s)	412, 489, 632, 1047, 1066
XceI	RCATGY	3 cut(s)	930, 1213, 1355
XcmI	CCANNNNNNNNNTGG	2 cut(s)	1027, 1306
XmiI	GTMKAC	2 cut(s)	1014, 1430
XspI	CTAG	4 cut(s)	11, 356, 830, 1376
ZraI	GACGTC	2 cut(s)	298, 337

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.