Rh4CG300900

Cation H( ) antiporter

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr4C
OG0004570
Physical Location & Seq
Forward (+)
56123821 .. 56124640
820 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh4CG300900.1

Sequence Viewer

Length: 627 bp
ATGGCGCCCGCCCCGCAGCGGGCGACCACCCCACCCATGAAGGCCACTTCAAATGGATCATTTCAGGGCGAAAATCCACTTGATTACATTCTTCCCTTACTCGTCGTCCAGATCATCTTGGTCGTGGTGTTCACCAGGTTTCTTGCATATCTTCTAAAACCTCTCCGGCAACCCCGAGTCATAGCCGAAGTTATATTTTTGAATCAATTTTTTTATGCATCCGTGCATCATTTGATAAACAAATTCAATGAATGCAGGGCGGATGTCTGCTGGGGCCCTCCGCATTCAGGAGGAGCAAGACCTTTCTACACACGATTTTCCCGCCAACATCTCTATTTCTTCTTCTTCCTCGTCGGCCTCGAGCTCGACATTGACGCCATCCGCCACACCGGAAAGAAGTCCCTCGGCATCGCCCTCTGCGAGATCACCCTCCCCTTCGTCCTCGGCATCGGTACCTCCGAAGCCCTACGCGCCACCGGCTCGAAAGGCATGAAACAAGGCCCCTTCCTCGTCTTCAACGGCGTCGCCCTCTCCATCACTGCCTTCCCCGTCCTGGCCCGCATGAGCTCAAGCTCCTCACCACCGATGTCAGCCGCATTGCCATGTTCATACATCGACCAGAATTAG

Protein Analysis

208

Amino Acids

22.94

Weight (kDa)

9.47

Isoelectric Point (pI)

45.74

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Na_H_Exchanger PF00999 45 - 188 4.5e-06 Sodium/hydrogen exchanger, transmembrane
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc65I GGTACC 1 cut(s) 452
AccB1I GGYRCC 2 cut(s) 4, 452
AccII CGCG 1 cut(s) 471
AciI CCGC 9 cut(s) 9, 14, 19, 260, 281, 322, 382, 559, 594
AclWI GGATC 1 cut(s) 64
AcsI RAATTY 1 cut(s) 242
AcyI GRCGYC 3 cut(s) 5, 375, 522
AfaI GTAC 1 cut(s) 454
AfiI CCNNNNNNNGG 4 cut(s) 18, 19, 287, 553
AgsI TTSAA 4 cut(s) 51, 202, 247, 517
AjnI CCWGG 2 cut(s) 134, 552
AluBI AGCT 3 cut(s) 364, 567, 573
AluI AGCT 3 cut(s) 364, 567, 573
Alw21I GWGCWC 2 cut(s) 366, 569
AlwI GGATC 1 cut(s) 64
Ama87I CYCGRG 2 cut(s) 174, 359
AoxI GGCC 5 cut(s) 42, 274, 355, 499, 555
ApaI GGGCCC 1 cut(s) 278
ApeKI GCWGC 1 cut(s) 16
ApoI RAATTY 1 cut(s) 242
Asp718I GGTACC 1 cut(s) 452
AspLEI GCGC 2 cut(s) 7, 473
AspS9I GGNCC 4 cut(s) 274, 275, 500, 556
AsuHPI GGTGA 3 cut(s) 124, 418, 570
AvaI CYCGRG 2 cut(s) 174, 359
BaeGI GKGCMC 1 cut(s) 278
BanI GGYRCC 2 cut(s) 4, 452
BanII GRGCYC 3 cut(s) 278, 366, 569
BbsI GAAGAC 1 cut(s) 505
Bbv12I GWGCWC 2 cut(s) 366, 569
BbvI GCAGC 1 cut(s) 28
BccI CCATC 2 cut(s) 386, 542
BceAI ACGGC 1 cut(s) 535
BciT130I CCWGG 2 cut(s) 136, 554
BfoI RGCGCY 1 cut(s) 8
BisI GCNGC 2 cut(s) 17, 594
BlsI GCNGC 2 cut(s) 18, 595
Bme1390I CCNGG 2 cut(s) 136, 554
BmeT110I CYCGRG 2 cut(s) 174, 359
BmgT120I GGNCC 4 cut(s) 274, 275, 500, 556
BmiI GGNNCC 5 cut(s) 6, 275, 276, 454, 502
BmrFI CCNGG 2 cut(s) 136, 554
BmsI GCATC 4 cut(s) 227, 235, 417, 456
BpiI GAAGAC 1 cut(s) 505
BpuEI CTTGAG 1 cut(s) 553
BsaHI GRCGYC 3 cut(s) 5, 375, 522
BsaJI CCNNGG 2 cut(s) 403, 442
BsaWI WCCGGW 1 cut(s) 389
Bsc4I CCNNNNNNNGG 4 cut(s) 18, 19, 287, 553
Bse118I RCCGGY 1 cut(s) 476
Bse3DI GCAATG 1 cut(s) 596
BseBI CCWGG 2 cut(s) 136, 554
BseDI CCNNGG 2 cut(s) 403, 442
BseGI GGATG 3 cut(s) 218, 268, 378
BseLI CCNNNNNNNGG 4 cut(s) 18, 19, 287, 553
BseMI GCAATG 1 cut(s) 596
BseRI GAGGAG 2 cut(s) 306, 565
BseSI GKGCMC 1 cut(s) 278
BseXI GCAGC 1 cut(s) 28
BseYI CCCAGC 1 cut(s) 270
Bsh1236I CGCG 1 cut(s) 471
BshFI GGCC 5 cut(s) 44, 276, 357, 501, 557
BshNI GGYRCC 2 cut(s) 4, 452
BsiHKAI GWGCWC 2 cut(s) 366, 569
BsiHKCI CYCGRG 2 cut(s) 174, 359
BsiSI CCGG 3 cut(s) 166, 390, 477
BslFI GGGAC 1 cut(s) 385
BslI CCNNNNNNNGG 4 cut(s) 18, 19, 287, 553
BsmFI GGGAC 1 cut(s) 385
BsmI GAATGC 2 cut(s) 257, 283
BsnI GGCC 5 cut(s) 44, 276, 357, 501, 557
BsoBI CYCGRG 2 cut(s) 174, 359
Bsp120I GGGCCC 1 cut(s) 274
Bsp1286I GDGCHC 3 cut(s) 278, 366, 569
Bsp143I GATC 3 cut(s) 56, 111, 423
BspACI CCGC 9 cut(s) 9, 14, 19, 260, 281, 322, 382, 559, 594
BspANI GGCC 5 cut(s) 44, 276, 357, 501, 557
BspFNI CGCG 1 cut(s) 471
BspLI GGNNCC 5 cut(s) 6, 275, 276, 454, 502
BspPI GGATC 1 cut(s) 64
BspT107I GGYRCC 2 cut(s) 4, 452
BsrDI GCAATG 1 cut(s) 596
BsrFI RCCGGY 1 cut(s) 476
BssAI RCCGGY 1 cut(s) 476
BssECI CCNNGG 2 cut(s) 403, 442
BssMI GATC 3 cut(s) 56, 111, 423
BssNI GRCGYC 3 cut(s) 5, 375, 522
Bst2UI CCWGG 2 cut(s) 136, 554
BstACI GRCGYC 3 cut(s) 5, 375, 522
BstC8I GCNNGC 3 cut(s) 9, 21, 559
BstF5I GGATG 3 cut(s) 218, 268, 378
BstFNI CGCG 1 cut(s) 471
BstH2I RGCGCY 1 cut(s) 8
BstHHI GCGC 2 cut(s) 7, 473
BstKTI GATC 3 cut(s) 59, 114, 426
BstMBI GATC 3 cut(s) 56, 111, 423
BstMWI GCNNNNNNNGC 4 cut(s) 13, 470, 477, 486
BstNI CCWGG 2 cut(s) 136, 554
BstSCI CCNGG 2 cut(s) 134, 552
BstSLI GKGCMC 1 cut(s) 278
BstUI CGCG 1 cut(s) 471
BstV1I GCAGC 1 cut(s) 28
BstV2I GAAGAC 1 cut(s) 505
BsuRI GGCC 5 cut(s) 44, 276, 357, 501, 557
BtgZI GCGATG 1 cut(s) 394
BtsCI GGATG 3 cut(s) 218, 268, 378
BtsI GCAGTG 1 cut(s) 537
BtsIMutI CAGTG 1 cut(s) 537
Cac8I GCNNGC 3 cut(s) 9, 21, 559
CfoI GCGC 2 cut(s) 7, 473
Cfr10I RCCGGY 1 cut(s) 476
Cfr13I GGNCC 4 cut(s) 274, 275, 500, 556
CseI GACGC 2 cut(s) 383, 511
CsiI ACCWGGT 1 cut(s) 134
Csp6I GTAC 1 cut(s) 453
CviAII CATG 4 cut(s) 37, 490, 562, 603
CviQI GTAC 1 cut(s) 453
DinI GGCGCC 1 cut(s) 6
DpnI GATC 3 cut(s) 58, 113, 425
DpnII GATC 3 cut(s) 56, 111, 423
EciI GGCGGA 2 cut(s) 275, 371
Ecl136II GAGCTC 2 cut(s) 364, 567
Eco24I GRGCYC 3 cut(s) 278, 366, 569
Eco53kI GAGCTC 2 cut(s) 364, 567
Eco88I CYCGRG 2 cut(s) 174, 359
EcoICRI GAGCTC 2 cut(s) 364, 567
EcoO109I RGGNCCY 3 cut(s) 274, 275, 500
EcoRII CCWGG 2 cut(s) 134, 552
EcoT22I ATGCAT 1 cut(s) 220
EcoT38I GRGCYC 3 cut(s) 278, 366, 569
EgeI GGCGCC 1 cut(s) 6
EheI GGCGCC 1 cut(s) 6
FaeI CATG 4 cut(s) 40, 493, 565, 606
FaiI YATR 9 cut(s) 38, 148, 182, 194, 216, 491, 563, 604, 610
FaqI GGGAC 1 cut(s) 385
FatI CATG 4 cut(s) 36, 489, 561, 602
FauI CCCGC 5 cut(s) 12, 16, 21, 329, 566
Fnu4HI GCNGC 2 cut(s) 17, 594
FokI GGATG 3 cut(s) 205, 275, 365
FriOI GRGCYC 3 cut(s) 278, 366, 569
Fsp4HI GCNGC 2 cut(s) 17, 594
GlaI GCGC 2 cut(s) 6, 472
GluI GCNGC 2 cut(s) 17, 594
GsaI CCCAGC 1 cut(s) 274
HaeII RGCGCY 1 cut(s) 8
HaeIII GGCC 5 cut(s) 44, 276, 357, 501, 557
HapII CCGG 3 cut(s) 166, 390, 477
HgaI GACGC 2 cut(s) 383, 511
HhaI GCGC 2 cut(s) 7, 473
Hin1I GRCGYC 3 cut(s) 5, 375, 522
Hin1II CATG 4 cut(s) 40, 493, 565, 606
Hin6I GCGC 2 cut(s) 5, 471
HinP1I GCGC 2 cut(s) 5, 471
HinfI GANTC 2 cut(s) 177, 202
HpaII CCGG 3 cut(s) 166, 390, 477
HphI GGTGA 3 cut(s) 124, 418, 570
Hpy166II GTNNAC 1 cut(s) 132
Hpy188I TCNGA 1 cut(s) 460
Hpy188III TCNNGA 2 cut(s) 109, 288
Hpy8I GTNNAC 1 cut(s) 132
Hpy99I CGWCG 3 cut(s) 107, 356, 527
HpyAV CCTTC 4 cut(s) 34, 445, 514, 553
HpyCH4V TGCA 4 cut(s) 146, 218, 226, 255
HpyF10VI GCNNNNNNNGC 4 cut(s) 13, 470, 477, 486
Hsp92I GRCGYC 3 cut(s) 5, 375, 522
Hsp92II CATG 4 cut(s) 40, 493, 565, 606
HspAI GCGC 2 cut(s) 5, 471
KasI GGCGCC 1 cut(s) 4
KpnI GGTACC 1 cut(s) 456
Kzo9I GATC 3 cut(s) 56, 111, 423
LmnI GCTCC 2 cut(s) 293, 578
Lsp1109I GCAGC 1 cut(s) 28
LweI GCATC 4 cut(s) 227, 235, 417, 456
MabI ACCWGGT 1 cut(s) 134
MalI GATC 3 cut(s) 58, 113, 425
MboI GATC 3 cut(s) 56, 111, 423
MboII GAAGA 6 cut(s) 83, 143, 331, 334, 337, 505
MhlI GDGCHC 3 cut(s) 278, 366, 569
MluCI AATT 3 cut(s) 206, 242, 622
Mly113I GGCGCC 1 cut(s) 5
MlyI GAGTC 1 cut(s) 186
Mph1103I ATGCAT 1 cut(s) 220
MslI CAYNNNNRTG 1 cut(s) 601
MspA1I CMGCKG 1 cut(s) 19
MspI CCGG 3 cut(s) 166, 390, 477
MspR9I CCNGG 2 cut(s) 136, 554
Mva1269I GAATGC 2 cut(s) 257, 283
MvaI CCWGG 2 cut(s) 136, 554
MvnI CGCG 1 cut(s) 471
MwoI GCNNNNNNNGC 4 cut(s) 13, 470, 477, 486
NarI GGCGCC 1 cut(s) 5
NdeII GATC 3 cut(s) 56, 111, 423
NlaIII CATG 4 cut(s) 40, 493, 565, 606
NlaIV GGNNCC 5 cut(s) 6, 275, 276, 454, 502
NmeAIII GCCGAG 2 cut(s) 384, 423
NsiI ATGCAT 1 cut(s) 220
PaeR7I CTCGAG 1 cut(s) 359
PcsI WCGNNNNNNNCGW 3 cut(s) 357, 417, 456
PctI GAATGC 2 cut(s) 257, 283
PfeI GAWTC 1 cut(s) 202
PkrI GCNGC 2 cut(s) 18, 595
PleI GAGTC 1 cut(s) 185
PluTI GGCGCC 1 cut(s) 8
PpsI GAGTC 1 cut(s) 185
Psp124BI GAGCTC 2 cut(s) 366, 569
Psp6I CCWGG 2 cut(s) 134, 552
PspFI CCCAGC 1 cut(s) 270
PspGI CCWGG 2 cut(s) 134, 552
PspN4I GGNNCC 5 cut(s) 6, 275, 276, 454, 502
PspOMI GGGCCC 1 cut(s) 274
PspPI GGNCC 4 cut(s) 274, 275, 500, 556
PspXI VCTCGAGB 1 cut(s) 359
RsaI GTAC 1 cut(s) 454
RsaNI GTAC 1 cut(s) 453
RseI CAYNNNNRTG 1 cut(s) 601
SacI GAGCTC 2 cut(s) 366, 569
SatI GCNGC 2 cut(s) 17, 594
Sau3AI GATC 3 cut(s) 56, 111, 423
Sau96I GGNCC 4 cut(s) 274, 275, 500, 556
SchI GAGTC 1 cut(s) 186
ScrFI CCNGG 2 cut(s) 136, 554
SduI GDGCHC 3 cut(s) 278, 366, 569
SetI ASST 7 cut(s) 140, 163, 304, 366, 458, 569, 575
SexAI ACCWGGT 1 cut(s) 134
SfaNI GCATC 4 cut(s) 227, 235, 417, 456
SfoI GGCGCC 1 cut(s) 6
Sfr274I CTCGAG 1 cut(s) 359
SlaI CTCGAG 1 cut(s) 359
SmiMI CAYNNNNRTG 1 cut(s) 601
SmlI CTYRAG 2 cut(s) 359, 568
SmoI CTYRAG 2 cut(s) 359, 568
Sse9I AATT 3 cut(s) 206, 242, 622
SsiI CCGC 9 cut(s) 9, 14, 19, 260, 281, 322, 382, 559, 594
SspDI GGCGCC 1 cut(s) 4
SstI GAGCTC 2 cut(s) 366, 569
StyD4I CCNGG 2 cut(s) 134, 552
TaqI TCGA 4 cut(s) 360, 366, 482, 615
TasI AATT 3 cut(s) 206, 242, 622
TauI GCSGC 1 cut(s) 596
TfiI GAWTC 1 cut(s) 202
TscAI CASTG 1 cut(s) 544
TseI GCWGC 1 cut(s) 16
TspDTI ATGAA 4 cut(s) 53, 264, 506, 597
TspGWI ACGGA 1 cut(s) 211
TspRI CASTG 1 cut(s) 544
XapI RAATTY 1 cut(s) 242
XhoI CTCGAG 1 cut(s) 359
Zsp2I ATGCAT 1 cut(s) 220
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.