Basic Information

Type: gene

Biological Identity

Species

rosa_samantha

Chromosome

Chr3A

Orthogroup ID

OG0004570

Physical Location & Seq

Genomic Coordinates Reverse (-)

28390420 .. 28394481

Gene Structure 4062 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

Rh3AG257000.1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2184 bp

ATGATTTTCCCCCCAAAAGGCATGACGGTGCTAGACACGGTAGCCAACATCGGGCTCTGCTTCTTCCTCTTCCTCGTCGGCCTTGAGCTCGACATCGGCGCCATCCGCCGCACAGGCAAGAAGTCCCTCGGCATCGCCTTCTGCGGGATCACCCTCCCCTTCGTCCTCGGCATCGGCACCTCCGAAGCCCTACGCGCCACTGTCTCGAAAGGTGTGAAACAAGGCCCCTTCCTCGTTTTCAACGGCGTCGCCCTCTCCATCACCGCCTTCCCCGTCCTCGCCCGCATCCTCGCTGAGCTCAAGCTCCTCACCACCGACGTCGGCCGCATCGCCATGTCCGCCGCCGCCGTCAACGACGTCGCCGCCTGGATCCTTCTAGCCCTCGCCATCGCCCTCACTGGCAACAACACCTCCCCGCTCGTCTCCGTCTGGGTCCTCCTCTGCGGTGCCGGCTTCGTCGGGTTCGCCGTCGTCGCCATCCGCCCCATACTGGTCATGATGGCGCGAAGGTCCCCAGAGGGCGAACCCGTGAAGGAGCTGTACATCTGCATCACTCTCTCGCTGGTTTTGGCTGCGGGGTTCGTGACGGACACGATTGGGATACACGCGCTGTTCGGCGCATTCGTGGTGGGGATCATAATGCCGAAGGAGGGTCCGTTCGCTGGTGTGTTGATAGAGAAGATTGAGGACCTGGTTTCGGGGCTTCTATTGCCGCTTTATTTTGTATCCAGTGGGCTCAAGACTAATTTTTTGACTATAAGCGGGGGGGTGTCGTGGGGTATGCTGGTGCTGGTGATATGTACAGCGTCGTTTGGGAAGATCGTCGGGACTGTCACTGTGTCGATGCTGTTCAAGGTGCCTTTCAGGGAAGCCTTGGCGCTTGGCTTCCTTATGAATACAAAGGGCTTGGTGGAGCTCATCGTCCTCAATATTGGCAAGGATAGAAAGGTACTGAACGAACAGACTTTTGCCATCTTTGTTTTGATGGCGTTGTTCACCACCTTCATCACCACACCACTAGTGATGGCAGTGTACAAACCAGCAAGAAAGGGAGCCCCTTACAAACTACGCACCATTTTTCGCAAAAGCCCAGACACAGAACTTCGAATATTGGCATGCTTCCACACCACCCGCAACATCCCCACCATTATCAACCTCATCGAGTCGTCTCGCGGCACCCACAAGCGCGGACGACTCACCATCTACGCCATGCACCTCATGGAGCTCTCGGAGCGGTCCTCTGCAATCTCCATGGTCCACAAAGCCCGCTATAATGGCCTCCCATTTTGGAACAAGAAGAGTGATGGAAATGACCAAATGGTTATTGCTTTTGAGGCCTACGAGCAGCTCAGCAGCGTCAAGATTCGGCCAGCCACCGCCATCTCTTCTTTAAACGACATGCACGAGGACATATGCGCCTGCGCGCACCAAAAAACTGTGGCAATGATTTTGTTGCCGTTTCACAAGCATCAGAAGCTGGACGGCACCATGGAGTCGGTCGGACACTCATTTCAAGTGGTGAACGAGCGTGTGCTCCGACATGCGCCTTGTTCGGTTGGGATTCTAGTTGATAGAGGACTCGGTGGCACCACGCAGGTCTCAGCTAGCGAAGTGTCGTTTACGGCGGTGGTGGCGTTCTTCGGAGGAAGAGATGACCGGGAGGCACTGGCTTATGGTATGAGACTGGCTGAACATCCTGGGATTGCGTTGACTGTTTTTAGATTCGTTGCTCCACAAGGGAAGACATTGAGATTTGGAGCGAAATTAGTTGGGATAACAGCTGACAACAAGAAAATACTGAAAGAAGAAGATAGTAGCCAAGAGAATGAAGAGGACGAGAAGTTCTTGGAGGAGTACAAGAATGTAAGAAGTAGCAATAAGGAAAAGGAAGAGTCCATGTTTTACGAGGAGAAGGTGGTGGACAGCAAAGGGGAGATCGCGGTGGCATTAAAGACTCTGAGCAAGCACAACTTGTTTATAGTAGGGAGAATGTCGTCGACTGTCCCGTTGGTAGAGTCGAGTGACTGTGCAGAGCTTGGGCCAATCGGAAGCTTCTTAGCCTCGTCAGACTTCTCTTCCACAGCTTCAGTTGTGGTGATTCAACAATATGATGCTACAGCGTCGCGGCCGCTTGTGGTGGAGGAAGCTGACTATGAGTTGCCGGAGTCGTCGTCTATGGTATAA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005768 GO:0005770 GO:0006810 GO:0006811 GO:0006812 GO:0006885 GO:0008150 GO:0012505 GO:0015672 GO:0031410 GO:0031982 GO:0042592 GO:0043226 GO:0043227 GO:0043229 GO:0044424 GO:0044444 GO:0044464 GO:0048878 GO:0050801 GO:0051179 GO:0051234 GO:0055067 GO:0055080 GO:0065007 GO:0065008 GO:0097708 GO:0098771

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

Na_H_Exchanger

Protein Analysis

727

Amino Acids

78.85

Weight (kDa)

8.37

Isoelectric Point (pI)

40.61

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Na_H_Exchanger	PF00999	8 - 341	8.6e-53	Sodium/hydrogen exchanger, transmembrane
CHX17_2nd	PF23256	396 - 536	2.2e-51	Plant cation/H(+) antiporter domain
CHX17_C	PF23259	541 - 704	5.1e-19	Plant cation/H(+) antiporter, C-terminal domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0004570)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT3G17630 AT3G17630
fragaria_vesca	FvH4_4g21880
malus_domestica	MD13G1136000.v1.1 MD16G1131400.v1.1
prunus_persica	Prupe.1G209400_v2.0.a1
pyrus_communis	pycom16g11580
rosa_chinensis	RchiOBHm_Chr3g0483861
rosa_laevigata	RLG00000007178 RLG00000023257
rosa_multiflora	Rmu_co8183874.1_g000001 Rmu_co8462775.1_g000001 Rmu_sc0004940.1_g000012 Rmu_sc0005901.1_g000001 Rmu_sc0009599.1_g000012
rosa_roxburghii	Rroxscaffold_5G00369850 Rroxscaffold_6G00398090
rosa_rugosa	Rorug03G0208200.1 Rorug03G0208300 Rorug04G0222700 Rorug04G0222800
rosa_samantha	Rh3AG257000 Rh3BG293400 Rh3CG291700 Rh3DG286700 Rh4AG279900 Rh4AG280100 Rh4BG285300 Rh4BG285500 Rh4CG300900
rosa_wichuraiana	Rw3G023230

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AatII	GACGTC	2 cut(s)	321, 360
Acc36I	ACCTGC	1 cut(s)	1585
AccB1I	GGYRCC	7 cut(s)	98, 176, 446, 856, 1175, 1484, 1586
AccBSI	CCGCTC	2 cut(s)	418, 1234
AccI	GTMKAC	1 cut(s)	1997
AccII	CGCG	8 cut(s)	195, 505, 608, 1173, 1188, 1424, 1940, 2125
AclWI	GGATC	4 cut(s)	155, 364, 377, 641
AcoI	YGGCCR	3 cut(s)	322, 1367, 2126
AcuI	CTGAAG	1 cut(s)	2070
AcyI	GRCGYC	4 cut(s)	99, 246, 318, 357
AfaI	GTAC	5 cut(s)	542, 802, 951, 1034, 1856
AfiI	CCNNNNNNNGG	7 cut(s)	17, 51, 144, 490, 650, 662, 697
AgsI	TTSAA	4 cut(s)	241, 853, 1514, 2102
AhlI	ACTAGT	1 cut(s)	1018
AjnI	CCWGG	3 cut(s)	365, 690, 1696
AloI	GAACNNNNNNTCC	4 cut(s)	641, 673, 1826, 1858
Alw21I	GWGCWC	5 cut(s)	90, 300, 918, 1227, 1536
Alw26I	GTCTC	5 cut(s)	208, 427, 1173, 1603, 1675
AlwI	GGATC	4 cut(s)	155, 364, 377, 641
AlwNI	CAGNNNCTG	1 cut(s)	1477
AoxI	GGCC	8 cut(s)	79, 223, 322, 1276, 1335, 1367, 2039, 2126
ApeKI	GCWGC	3 cut(s)	572, 1345, 1353
ArsI	GACNNNNNNTTYG	2 cut(s)	733, 765
AspS9I	GGNCC	8 cut(s)	224, 433, 510, 653, 688, 1236, 1255, 2039
AsuC2I	CCSGG	1 cut(s)	1658
AsuHPI	GGTGA	9 cut(s)	142, 253, 301, 805, 988, 1000, 1189, 1531, 2107
AsuII	TTCGAA	1 cut(s)	1105
AsuNHI	GCTAGC	1 cut(s)	1604
AvaII	GGWCC	6 cut(s)	433, 510, 653, 688, 1236, 1255
BamHI	GGATCC	1 cut(s)	369
BanI	GGYRCC	7 cut(s)	98, 176, 446, 856, 1175, 1484, 1586
BanII	GRGCYC	7 cut(s)	57, 90, 300, 738, 918, 1057, 1227
BarI	GAAGNNNNNNTAC	2 cut(s)	524, 556
BauI	CACGAG	1 cut(s)	1403
BbsI	GAAGAC	1 cut(s)	1748
Bbv12I	GWGCWC	5 cut(s)	90, 300, 918, 1227, 1536
BbvI	GCAGC	3 cut(s)	559, 1357, 1365
BceAI	ACGGC	6 cut(s)	259, 332, 452, 1441, 1498, 1638
BciT130I	CCWGG	3 cut(s)	367, 692, 1698
BciVI	GTATCC	2 cut(s)	594, 736
BcnI	CCSGG	1 cut(s)	1658
BcoDI	GTCTC	5 cut(s)	208, 427, 1173, 1603, 1675
BcuI	ACTAGT	1 cut(s)	1018
BfaI	CTAG	5 cut(s)	32, 377, 1019, 1565, 1605
BfmI	CTRYAG	1 cut(s)	2115
BfoI	RGCGCY	2 cut(s)	102, 881
BfuAI	ACCTGC	1 cut(s)	1585
BfuI	GTATCC	2 cut(s)	594, 736
BglI	GCCNNNNNGGC	1 cut(s)	114
BlpI	GCTNAGC	2 cut(s)	294, 1349
Bme1390I	CCNGG	4 cut(s)	367, 692, 1658, 1698
Bme18I	GGWCC	6 cut(s)	433, 510, 653, 688, 1236, 1255
BmgT120I	GGNCC	8 cut(s)	224, 433, 510, 653, 688, 1236, 1255, 2039
BmrFI	CCNGG	4 cut(s)	367, 692, 1658, 1698
BmsI	GCATC	8 cut(s)	141, 180, 294, 336, 558, 834, 1477, 2101
BmtI	GCTAGC	1 cut(s)	1608
BpiI	GAAGAC	1 cut(s)	1748
BplI	GAGNNNNNCTC	2 cut(s)	1223, 1255
Bpu1102I	GCTNAGC	2 cut(s)	294, 1349
Bpu14I	TTCGAA	1 cut(s)	1105
BpuEI	CTTGAG	3 cut(s)	104, 284, 722
BpuMI	CCSGG	1 cut(s)	1658
BsaHI	GRCGYC	4 cut(s)	99, 246, 318, 357
BsaI	GGTCTC	1 cut(s)	1603
BsaJI	CCNNGG	6 cut(s)	127, 166, 873, 1251, 1488, 1697
BsaXI	ACNNNNNCTCC	5 cut(s)	395, 425, 641, 671, 2156
Bsc4I	CCNNNNNNNGG	7 cut(s)	17, 51, 144, 490, 650, 662, 697
Bse118I	RCCGGY	1 cut(s)	449
Bse1I	ACTGG	5 cut(s)	403, 495, 729, 1671, 1689
Bse3DI	GCAATG	1 cut(s)	1449
BseBI	CCWGG	3 cut(s)	367, 692, 1698
BseDI	CCNNGG	6 cut(s)	127, 166, 873, 1251, 1488, 1697
BseGI	GGATG	5 cut(s)	102, 285, 477, 1137, 1693
BseLI	CCNNNNNNNGG	7 cut(s)	17, 51, 144, 490, 650, 662, 697
BseMI	GCAATG	1 cut(s)	1449
BseMII	CTCAG	4 cut(s)	285, 1363, 1614, 1949
BseNI	ACTGG	5 cut(s)	403, 495, 729, 1671, 1689
BsePI	GCGCGC	1 cut(s)	1422
BseRI	GAGGAG	4 cut(s)	296, 428, 1865, 1922
BseX3I	CGGCCG	2 cut(s)	322, 2126
BseXI	GCAGC	3 cut(s)	559, 1357, 1365
BsgI	GTGCAG	1 cut(s)	2049
Bsh1236I	CGCG	8 cut(s)	195, 505, 608, 1173, 1188, 1424, 1940, 2125
Bsh1285I	CGRYCG	3 cut(s)	325, 1500, 2129
BshFI	GGCC	8 cut(s)	81, 225, 324, 1278, 1337, 1369, 2041, 2128
BshNI	GGYRCC	7 cut(s)	98, 176, 446, 856, 1175, 1484, 1586
BsiEI	CGRYCG	3 cut(s)	325, 1500, 2129
BsiHKAI	GWGCWC	5 cut(s)	90, 300, 918, 1227, 1536
BsiSI	CCGG	3 cut(s)	450, 1657, 2162
BslFI	GGGAC	4 cut(s)	109, 496, 841, 1988
BslI	CCNNNNNNNGG	7 cut(s)	17, 51, 144, 490, 650, 662, 697
BsmAI	GTCTC	5 cut(s)	208, 427, 1173, 1603, 1675
BsmBI	CGTCTC	2 cut(s)	427, 1173
BsmFI	GGGAC	4 cut(s)	109, 496, 841, 1988
BsmI	GAATGC	1 cut(s)	620
BsnI	GGCC	8 cut(s)	81, 225, 324, 1278, 1337, 1369, 2041, 2128
Bso31I	GGTCTC	1 cut(s)	1603
Bsp119I	TTCGAA	1 cut(s)	1105
Bsp1286I	GDGCHC	8 cut(s)	57, 90, 300, 738, 918, 1057, 1227, 1536
Bsp1407I	TGTACA	3 cut(s)	540, 800, 1032
Bsp143I	GATC	5 cut(s)	147, 369, 633, 819, 1935
Bsp1720I	GCTNAGC	2 cut(s)	294, 1349
Bsp19I	CCATGG	2 cut(s)	1251, 1488
BspANI	GGCC	8 cut(s)	81, 225, 324, 1278, 1337, 1369, 2041, 2128
BspCNI	CTCAG	4 cut(s)	286, 1362, 1613, 1950
BspFNI	CGCG	8 cut(s)	195, 505, 608, 1173, 1188, 1424, 1940, 2125
BspHI	TCATGA	1 cut(s)	495
BspMI	ACCTGC	1 cut(s)	1585
BspOI	GCTAGC	1 cut(s)	1608
BspPI	GGATC	4 cut(s)	155, 364, 377, 641
BspT104I	TTCGAA	1 cut(s)	1105
BspT107I	GGYRCC	7 cut(s)	98, 176, 446, 856, 1175, 1484, 1586
BspTNI	GGTCTC	1 cut(s)	1603
BsrBI	CCGCTC	2 cut(s)	418, 1234
BsrDI	GCAATG	1 cut(s)	1449
BsrFI	RCCGGY	1 cut(s)	449
BsrGI	TGTACA	3 cut(s)	540, 800, 1032
BsrI	ACTGG	5 cut(s)	403, 495, 729, 1671, 1689
BssAI	RCCGGY	1 cut(s)	449
BssECI	CCNNGG	6 cut(s)	127, 166, 873, 1251, 1488, 1697
BssHII	GCGCGC	1 cut(s)	1422
BssMI	GATC	5 cut(s)	147, 369, 633, 819, 1935
BssNI	GRCGYC	4 cut(s)	99, 246, 318, 357
BssSI	CACGAG	1 cut(s)	1403
BssT1I	CCWWGG	3 cut(s)	873, 1251, 1488
Bst2BI	CACGAG	1 cut(s)	1403
Bst2UI	CCWGG	3 cut(s)	367, 692, 1698
Bst4CI	ACNGT	9 cut(s)	28, 40, 202, 832, 838, 1438, 1714, 2002, 2027
Bst6I	CTCTTC	7 cut(s)	74, 1292, 1390, 1642, 1824, 1884, 2080
BstACI	GRCGYC	4 cut(s)	99, 246, 318, 357
BstAUI	TGTACA	3 cut(s)	540, 800, 1032
BstBI	TTCGAA	1 cut(s)	1105
BstC8I	GCNNGC	9 cut(s)	283, 451, 1117, 1267, 1371, 1420, 1424, 1606, 1964
BstDEI	CTNAG	5 cut(s)	294, 1349, 1600, 1958, 2056
BstDSI	CCRYGG	2 cut(s)	1251, 1488
BstF5I	GGATG	5 cut(s)	102, 285, 477, 1137, 1693
BstFNI	CGCG	8 cut(s)	195, 505, 608, 1173, 1188, 1424, 1940, 2125
BstH2I	RGCGCY	2 cut(s)	102, 881
BstKTI	GATC	5 cut(s)	150, 372, 636, 822, 1938
BstMAI	GTCTC	5 cut(s)	208, 427, 1173, 1603, 1675
BstMBI	GATC	5 cut(s)	147, 369, 633, 819, 1935
BstMCI	CGRYCG	3 cut(s)	325, 1500, 2129
BstNI	CCWGG	3 cut(s)	367, 692, 1698
BstNSI	RCATGY	3 cut(s)	1119, 1402, 1544
BstSCI	CCNGG	4 cut(s)	365, 690, 1656, 1696
BstSFI	CTRYAG	1 cut(s)	2115
BstUI	CGCG	8 cut(s)	195, 505, 608, 1173, 1188, 1424, 1940, 2125
BstV1I	GCAGC	3 cut(s)	559, 1357, 1365
BstV2I	GAAGAC	1 cut(s)	1748
BstX2I	RGATCY	1 cut(s)	369
BstYI	RGATCY	1 cut(s)	369
BstZI	CGGCCG	2 cut(s)	322, 2126
BsuI	GTATCC	2 cut(s)	594, 736
BsuRI	GGCC	8 cut(s)	81, 225, 324, 1278, 1337, 1369, 2041, 2128
BtgI	CCRYGG	2 cut(s)	1251, 1488
BtgZI	GCGATG	3 cut(s)	118, 313, 373
BtsCI	GGATG	5 cut(s)	102, 285, 477, 1137, 1693
BtsI	GCAGTG	1 cut(s)	1035
BtsIMutI	CAGTG	6 cut(s)	198, 396, 736, 834, 1035, 1664
BveI	ACCTGC	1 cut(s)	1585
Cac8I	GCNNGC	9 cut(s)	283, 451, 1117, 1267, 1371, 1420, 1424, 1606, 1964
CaiI	CAGNNNCTG	1 cut(s)	1477
CciI	TCATGA	1 cut(s)	495
CciNI	GCGGCCGC	1 cut(s)	2126
Cfr10I	RCCGGY	1 cut(s)	449
Cfr13I	GGNCC	8 cut(s)	224, 433, 510, 653, 688, 1236, 1255, 2039
CseI	GACGC	4 cut(s)	235, 795, 1345, 2109
CsiI	ACCWGGT	1 cut(s)	690
Csp6I	GTAC	5 cut(s)	541, 801, 950, 1033, 1855
CviQI	GTAC	5 cut(s)	541, 801, 950, 1033, 1855
DdeI	CTNAG	5 cut(s)	294, 1349, 1600, 1958, 2056
DinI	GGCGCC	1 cut(s)	100
DpnI	GATC	5 cut(s)	149, 371, 635, 821, 1937
DpnII	GATC	5 cut(s)	147, 369, 633, 819, 1935
DraI	TTTAAA	1 cut(s)	1392
EaeI	YGGCCR	3 cut(s)	322, 1367, 2126
EagI	CGGCCG	2 cut(s)	322, 2126
Eam1104I	CTCTTC	7 cut(s)	74, 1292, 1390, 1642, 1824, 1884, 2080
EarI	CTCTTC	7 cut(s)	74, 1292, 1390, 1642, 1824, 1884, 2080
EciI	GGCGGA	3 cut(s)	95, 328, 470
Ecl136II	GAGCTC	4 cut(s)	88, 298, 916, 1225
EclXI	CGGCCG	2 cut(s)	322, 2126
Eco130I	CCWWGG	3 cut(s)	873, 1251, 1488
Eco147I	AGGCCT	1 cut(s)	1337
Eco24I	GRGCYC	7 cut(s)	57, 90, 300, 738, 918, 1057, 1227
Eco31I	GGTCTC	1 cut(s)	1603
Eco47I	GGWCC	6 cut(s)	433, 510, 653, 688, 1236, 1255
Eco52I	CGGCCG	2 cut(s)	322, 2126
Eco53kI	GAGCTC	4 cut(s)	88, 298, 916, 1225
Eco57I	CTGAAG	1 cut(s)	2070
EcoICRI	GAGCTC	4 cut(s)	88, 298, 916, 1225
EcoO109I	RGGNCCY	4 cut(s)	224, 433, 510, 688
EcoRII	CCWGG	3 cut(s)	365, 690, 1696
EcoT14I	CCWWGG	3 cut(s)	873, 1251, 1488
EcoT38I	GRGCYC	7 cut(s)	57, 90, 300, 738, 918, 1057, 1227
EgeI	GGCGCC	1 cut(s)	100
EheI	GGCGCC	1 cut(s)	100
ErhI	CCWWGG	3 cut(s)	873, 1251, 1488
Esp3I	CGTCTC	2 cut(s)	427, 1173
FalI	AAGNNNNNCTT	2 cut(s)	1955, 1987
FaqI	GGGAC	4 cut(s)	109, 496, 841, 1988
FauI	CCCGC	7 cut(s)	137, 290, 423, 568, 755, 1139, 1274
FauNDI	CATATG	1 cut(s)	1412
FblI	GTMKAC	1 cut(s)	1997
FokI	GGATG	5 cut(s)	89, 272, 464, 1124, 1680
FriOI	GRGCYC	7 cut(s)	57, 90, 300, 738, 918, 1057, 1227
FspBI	CTAG	5 cut(s)	32, 377, 1019, 1565, 1605
HaeII	RGCGCY	2 cut(s)	102, 881
HaeIII	GGCC	8 cut(s)	81, 225, 324, 1278, 1337, 1369, 2041, 2128
HapII	CCGG	3 cut(s)	450, 1657, 2162
HgaI	GACGC	4 cut(s)	235, 795, 1345, 2109
Hin1I	GRCGYC	4 cut(s)	99, 246, 318, 357
HincII	GTYRAC	3 cut(s)	352, 1710, 1998
HindII	GTYRAC	3 cut(s)	352, 1710, 1998
HindIII	AAGCTT	1 cut(s)	2050
HpaII	CCGG	3 cut(s)	450, 1657, 2162
HphI	GGTGA	9 cut(s)	142, 253, 301, 805, 988, 1000, 1189, 1531, 2107
Hpy166II	GTNNAC	9 cut(s)	352, 996, 1033, 1258, 1522, 1620, 1710, 1921, 1998
Hpy188I	TCNGA	9 cut(s)	184, 1231, 1473, 1502, 1538, 1643, 1959, 2048, 2068
Hpy188III	TCNNGA	6 cut(s)	205, 496, 583, 739, 826, 1360
Hpy8I	GTNNAC	9 cut(s)	352, 996, 1033, 1258, 1522, 1620, 1710, 1921, 1998
HpyCH4III	ACNGT	9 cut(s)	28, 40, 202, 832, 838, 1438, 1714, 2002, 2027
HpyCH4IV	ACGT	2 cut(s)	318, 357
HpyCH4V	TGCA	5 cut(s)	549, 1213, 1244, 1402, 2030
HpyF3I	CTNAG	5 cut(s)	294, 1349, 1600, 1958, 2056
HpySE526I	ACGT	2 cut(s)	318, 357
Hsp92I	GRCGYC	4 cut(s)	99, 246, 318, 357
KasI	GGCGCC	1 cut(s)	98
KroI	GCCGGC	1 cut(s)	449
KroNI	GCCGGC	1 cut(s)	451
Kzo9I	GATC	5 cut(s)	147, 369, 633, 819, 1935
LmnI	GCTCC	9 cut(s)	309, 535, 913, 1052, 1222, 1231, 1539, 1735, 1757
Lsp1109I	GCAGC	3 cut(s)	559, 1357, 1365
LweI	GCATC	8 cut(s)	141, 180, 294, 336, 558, 834, 1477, 2101
MabI	ACCWGGT	1 cut(s)	690
MaeI	CTAG	5 cut(s)	32, 377, 1019, 1565, 1605
MaeII	ACGT	2 cut(s)	318, 357
MaeIII	GTNAC	3 cut(s)	583, 832, 2021
MalI	GATC	5 cut(s)	149, 371, 635, 821, 1937
MbiI	CCGCTC	2 cut(s)	418, 1234
MboI	GATC	5 cut(s)	147, 369, 633, 819, 1935
MflI	RGATCY	1 cut(s)	369
MhlI	GDGCHC	8 cut(s)	57, 90, 300, 738, 918, 1057, 1227, 1536
MluCI	AATT	2 cut(s)	745, 1763
Mly113I	GGCGCC	1 cut(s)	99
MlyI	GAGTC	8 cut(s)	1172, 1188, 1502, 1572, 1901, 1948, 2024, 2174
MmeI	TCCRAC	2 cut(s)	1480, 1561
MroNI	GCCGGC	1 cut(s)	449
MseI	TTAA	2 cut(s)	1391, 1949
MslI	CAYNNNNRTG	2 cut(s)	26, 332
MspA1I	CMGCKG	1 cut(s)	1781
MspI	CCGG	3 cut(s)	450, 1657, 2162
MspR9I	CCNGG	4 cut(s)	367, 692, 1658, 1698
Mva1269I	GAATGC	1 cut(s)	620
MvaI	CCWGG	3 cut(s)	367, 692, 1698
MvnI	CGCG	8 cut(s)	195, 505, 608, 1173, 1188, 1424, 1940, 2125
NaeI	GCCGGC	1 cut(s)	451
NarI	GGCGCC	1 cut(s)	99
NciI	CCSGG	1 cut(s)	1658
NcoI	CCATGG	2 cut(s)	1251, 1488
NdeI	CATATG	1 cut(s)	1412
NdeII	GATC	5 cut(s)	147, 369, 633, 819, 1935
NgoMIV	GCCGGC	1 cut(s)	449
NheI	GCTAGC	1 cut(s)	1604
NmeAIII	GCCGAG	2 cut(s)	108, 147
NmuCI	GTSAC	3 cut(s)	583, 832, 2021
NotI	GCGGCCGC	1 cut(s)	2126
NspI	RCATGY	3 cut(s)	1119, 1402, 1544
NspV	TTCGAA	1 cut(s)	1105
PaeI	GCATGC	1 cut(s)	1119
PagI	TCATGA	1 cut(s)	495
PauI	GCGCGC	1 cut(s)	1422
PceI	AGGCCT	1 cut(s)	1337
PcsI	WCGNNNNNNNCGW	5 cut(s)	180, 465, 621, 1401, 2003
PctI	GAATGC	1 cut(s)	620
PdiI	GCCGGC	1 cut(s)	451
PfeI	GAWTC	4 cut(s)	1363, 1561, 1722, 2098
PleI	GAGTC	8 cut(s)	1171, 1188, 1501, 1572, 1900, 1948, 2023, 2173
PluTI	GGCGCC	1 cut(s)	102
PpsI	GAGTC	8 cut(s)	1171, 1188, 1501, 1572, 1900, 1948, 2023, 2173
PpuMI	RGGWCCY	3 cut(s)	433, 510, 688
Psp124BI	GAGCTC	4 cut(s)	90, 300, 918, 1227
Psp5II	RGGWCCY	3 cut(s)	433, 510, 688
Psp6I	CCWGG	3 cut(s)	365, 690, 1696
PspGI	CCWGG	3 cut(s)	365, 690, 1696
PspPI	GGNCC	8 cut(s)	224, 433, 510, 653, 688, 1236, 1255, 2039
PspPPI	RGGWCCY	3 cut(s)	433, 510, 688
PstNI	CAGNNNCTG	1 cut(s)	1477
PsuI	RGATCY	1 cut(s)	369
PteI	GCGCGC	1 cut(s)	1422
PvuII	CAGCTG	1 cut(s)	1781
RsaI	GTAC	5 cut(s)	542, 802, 951, 1034, 1856
RsaNI	GTAC	5 cut(s)	541, 801, 950, 1033, 1855
RseI	CAYNNNNRTG	2 cut(s)	26, 332
SacI	GAGCTC	4 cut(s)	90, 300, 918, 1227
SalI	GTCGAC	1 cut(s)	1996
SaqAI	TTAA	2 cut(s)	1391, 1949
Sau3AI	GATC	5 cut(s)	147, 369, 633, 819, 1935
Sau96I	GGNCC	8 cut(s)	224, 433, 510, 653, 688, 1236, 1255, 2039
SchI	GAGTC	8 cut(s)	1172, 1188, 1502, 1572, 1901, 1948, 2024, 2174
ScrFI	CCNGG	4 cut(s)	367, 692, 1658, 1698
SduI	GDGCHC	8 cut(s)	57, 90, 300, 738, 918, 1057, 1227, 1536
SexAI	ACCWGGT	1 cut(s)	690
SfaNI	GCATC	8 cut(s)	141, 180, 294, 336, 558, 834, 1477, 2101
SfcI	CTRYAG	1 cut(s)	2115
SfoI	GGCGCC	1 cut(s)	100
SfuI	TTCGAA	1 cut(s)	1105
SinI	GGWCC	6 cut(s)	433, 510, 653, 688, 1236, 1255
SmiMI	CAYNNNNRTG	2 cut(s)	26, 332
SmlI	CTYRAG	3 cut(s)	83, 299, 737
SmoI	CTYRAG	3 cut(s)	83, 299, 737
SpeI	ACTAGT	1 cut(s)	1018
SphI	GCATGC	1 cut(s)	1119
Sse9I	AATT	2 cut(s)	745, 1763
SseBI	AGGCCT	1 cut(s)	1337
SspDI	GGCGCC	1 cut(s)	98
SspI	AATATT	2 cut(s)	931, 1110
SspMI	CTAG	5 cut(s)	32, 377, 1019, 1565, 1605
SstI	GAGCTC	4 cut(s)	90, 300, 918, 1227
StuI	AGGCCT	1 cut(s)	1337
StyD4I	CCNGG	4 cut(s)	365, 690, 1656, 1696
StyI	CCWWGG	3 cut(s)	873, 1251, 1488
TaaI	ACNGT	9 cut(s)	28, 40, 202, 832, 838, 1438, 1714, 2002, 2027
TaiI	ACGT	2 cut(s)	321, 360
TaqI	TCGA	7 cut(s)	90, 206, 842, 1105, 1161, 1997, 2018
TaqII	GACCGA	1 cut(s)	1486
TasI	AATT	2 cut(s)	745, 1763
TatI	WGTACW	4 cut(s)	540, 800, 1032, 1854
TauI	GCSGC	9 cut(s)	111, 327, 344, 347, 365, 715, 1176, 2128, 2131
TfiI	GAWTC	4 cut(s)	1363, 1561, 1722, 2098
Tru1I	TTAA	2 cut(s)	1391, 1949
Tru9I	TTAA	2 cut(s)	1391, 1949
TscAI	CASTG	6 cut(s)	205, 403, 736, 841, 1035, 1671
TseFI	GTSAC	3 cut(s)	583, 832, 2021
TseI	GCWGC	3 cut(s)	572, 1345, 1353
Tsp45I	GTSAC	3 cut(s)	583, 832, 2021
TspDTI	ATGAA	3 cut(s)	908, 994, 1842
TspGWI	ACGGA	3 cut(s)	415, 602, 645
TspRI	CASTG	6 cut(s)	205, 403, 736, 841, 1035, 1671
VpaK11BI	GGWCC	6 cut(s)	433, 510, 653, 688, 1236, 1255
XceI	RCATGY	3 cut(s)	1119, 1402, 1544
XcmI	CCANNNNNNNNNTGG	1 cut(s)	1216
XmiI	GTMKAC	1 cut(s)	1997
XspI	CTAG	5 cut(s)	32, 377, 1019, 1565, 1605
ZraI	GACGTC	2 cut(s)	319, 358

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.