Basic Information

Type: gene

Biological Identity

Species

rosa_laevigata

Chromosome

Chr5

Orthogroup ID

OG0004570

Physical Location & Seq

Genomic Coordinates Forward (+)

20693907 .. 20697207

Gene Structure 3301 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

RLM00000023257

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2406 bp

ATGGCACCTGCGCCGCTGCCGGCGACCACCCCGCCCATGAAGGCCACTTCAAATGGATCATTTCAAGGTGAAAATCCACTTGATTACGCTCTTCCCTTACTCATCCTCCAAATCATCTTGGTCGTGGTGTTCACCAGGTTTCTCGCATATCTTCTAAAACCTCTCCGGCAACCCCGAGTCATAGCCGAAGTTATAGGCGGATGTCTGCTGGGCCCCTCCGCATTCGGGAGGAGCAAGACCTTTCTACACATGATTTTCCCGCCAAAAGGCATGACGGTGCTGGACACGGTAGCCAACATAGGGCTCTGCTTCTTCCTCTTCCTCGTCGGCCTTGAGCTCGACATCAGCGCCATCCGCCGCACAGGCAAGAAGTCCCTCGGCATCGCCTTCTGCGGGATCACCCTCCCCTTCGTCCTCGGCATCGGCACCTCTGAAGCCCTACGCGCCACCGTCTCGAAAGGCGTGAAACAAGGCCCCTTCCTCGTTTTCAACGGCGTCGCCCTCTCCATCACCGCCTTCCCAGTCCTCGCCCGCATCCTCGCTGAGCTCAAGCTCCTCACCACCGACGTCGGCCGCATCGCCATGTCCGCTGCCGCCGTCAACGACGTCGCCGCCTGGATCCTTCTAGCCCTCGCCATCGCCCTCACCGGCAACAACACCTCCCCGCTCGTCTCCGTCTGGGTCCTCCTCTGCGGTGCCGGATTCGTCGGGTTCGCCGTCTTCGCCATCCGCCCCATACTAGTCATGATGGCGAGAAGATCCCAGGAGGGCGAACCCGTGAAGGAGCTCTACATTTGCGTCACTCTCTCGCTAGTTTTGGCCGCGGGGTTCGTGACGGACACGATTGGGATACACGCGCTGTTCGGCGCGTTTGTGGTGGGGATCATAATGCCGAAGGAGGGTCCGTTCGCTGGTGTGTTGATAGAGAAGATTGAGGACCTGGTTTCGGGGCTTCTATTGCCGCTTTATTTTGTATCCAGTGGGCTCAAGACTAATTTTTTGACTATAAGCGGGGGGGTGTCGTGGGGTATGCTGGTGCTGGTGATATGCACAGCGTCGTTTGGGAAGATCGTCGGGACTGTCACCGTGTCGATGTTGTTCAAGGTGCCTTTCAGGGAAGCCTTGGCGCTTGGCTTCCTTATGAATACAAAGGGCTTGGTGGAGCTCATCGTACTGAATGAACAGACTTTTGCCATCTTTGTTTTGATGGCGTTGTTCACCACCTTCATCACCACACCACTAGTGATGGCAGTGTACAAACCAGCAAGAAAGGGAGCCCCTTACAAACTACGCACCATTTTCCGCAAAAGCCCAGACACAGAACTTCGAATATTGGCATGCTTCCACACCACCCGCAACATCCCCACCATGATCAACCTCATCGAGTCGTCTCGCGGCACCCACAAGCGCGGAAGACTCACCGTCTACGCCATGCACCTCATGGAGCTCTCGGAGCGGTCCTCTGCCATCTCCATGGTCCATAAAGCCCGCTATAATGGCCTCCCATTTTGGAACAAGAAGAGTGATGGAAATGACCAAATGGTTATTGCTTTCGAGGCCTACGAGCAGCTCAGCAGCGTCAAGATTCGGCCAGCCACCGCCATCTCTTCTTTAAACGACATGCACGAGGACATATGCGCCTGCGCGCACCAAAAAACCGTGGCAATGATTTTGTTGCCATTTCACAAGCATCAGAAGCTGGACGGCACCATGGAGTCAGTGGGACACTCATTTCAAGTGGTGAACGAGCGTGTGCTTCGACATGCGCCTTGTTCGGTTGGGATTCTAGTTGATAGAGGACTCGGTGGCACCACGCAAGTCTCAGCCAGCGACGTGTCATATACGGCGGTGGTGGCGTTCTTCGGAGGAAGAGATGATCGGGAGGCACTGGCTTATGGTATGAGACTGGCTGAGCATCCTGGGATTGCGTTGACTGTTTTTAGATTCGTTGCTCCACAAGGGAAGACATTGAGATTTGGAGCAAAATTAATTGGGATAACTGCTGATAATAAGAAAATACTGAAAGAAGAAGATAGCAGCCAAGAGAATGAAGAGGACGAGAAGTTTTTGGAGGAGTACAAGAATGTAAGAAGTAGCAGTAAGGAAAAGGAAGAGTCCATGTTTTACGAGGAGAAGGTGGTGGACAGCAAAGGGGAGATCGCGGTGGCGTTAAAGACTCTGAGCAAGCACAACTTGTTTATAGTAGGGAGAATGTCGCCGACTGTCCCGTTGGTAGAGTCGAGTGACTGTGCGGAGCTTGGGCCAATCGGAAGCTTCTTAGCCTCGTCAGACTTCTCTTCCACAGCTTCAGTTGTGGTGATTCAACAGTATGATGCTACAGCGTCGCGGCCGCTTGTGGTGGAGGAAGTTGACTATGAGTTGCCGGAGTCGTCGTCTATGGTTTAA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005768 GO:0005770 GO:0006810 GO:0006811 GO:0006812 GO:0006885 GO:0008150 GO:0012505 GO:0015672 GO:0031410 GO:0031982 GO:0042592 GO:0043226 GO:0043227 GO:0043229 GO:0044424 GO:0044444 GO:0044464 GO:0048878 GO:0050801 GO:0051179 GO:0051234 GO:0055067 GO:0055080 GO:0065007 GO:0065008 GO:0097708 GO:0098771

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

Na_H_Exchanger

Protein Analysis

802

Amino Acids

86.9

Weight (kDa)

8.58

Isoelectric Point (pI)

41.37

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Na_H_Exchanger	PF00999	44 - 414	8.5e-53	Sodium/hydrogen exchanger, transmembrane
CHX17_2nd	PF23256	470 - 610	2.9e-51	Plant cation/H(+) antiporter domain
CHX17_C	PF23259	616 - 778	2.1e-18	Plant cation/H(+) antiporter, C-terminal domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0004570)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT3G17630 AT3G17630
fragaria_vesca	FvH4_4g21880
malus_domestica	MD13G1136000.v1.1 MD16G1131400.v1.1
prunus_persica	Prupe.1G209400_v2.0.a1
pyrus_communis	pycom16g11580
rosa_chinensis	RchiOBHm_Chr3g0483861
rosa_laevigata	RLG00000007178 RLG00000023257
rosa_multiflora	Rmu_co8183874.1_g000001 Rmu_co8462775.1_g000001 Rmu_sc0004940.1_g000012 Rmu_sc0005901.1_g000001 Rmu_sc0009599.1_g000012
rosa_roxburghii	Rroxscaffold_5G00369850 Rroxscaffold_6G00398090
rosa_rugosa	Rorug03G0208200.1 Rorug03G0208300 Rorug04G0222700 Rorug04G0222800
rosa_samantha	Rh3AG257000 Rh3BG293400 Rh3CG291700 Rh3DG286700 Rh4AG279900 Rh4AG280100 Rh4BG285300 Rh4BG285500 Rh4CG300900
rosa_wichuraiana	Rw3G023230

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AarI	CACCTGC	1 cut(s)	16
AatII	GACGTC	2 cut(s)	570, 609
Acc36I	ACCTGC	1 cut(s)	16
AccB1I	GGYRCC	7 cut(s)	4, 425, 695, 1105, 1397, 1706, 1808
AccBSI	CCGCTC	2 cut(s)	667, 1456
AccI	GTMKAC	1 cut(s)	1425
AccII	CGCG	9 cut(s)	444, 824, 857, 869, 1395, 1410, 1646, 2162, 2347
AclWI	GGATC	6 cut(s)	64, 404, 613, 626, 753, 890
AcoI	YGGCCR	4 cut(s)	571, 819, 1589, 2348
AcuI	CTGAAG	2 cut(s)	453, 2292
AcyI	GRCGYC	3 cut(s)	495, 567, 606
AfaI	GTAC	3 cut(s)	1173, 1256, 2078
AfiI	CCNNNNNNNGG	7 cut(s)	225, 266, 300, 393, 899, 911, 946
AflIII	ACRYGT	1 cut(s)	1833
AgsI	TTSAA	6 cut(s)	51, 65, 490, 1102, 1736, 2324
AhdI	GACNNNNNGTC	1 cut(s)	1421
AhlI	ACTAGT	2 cut(s)	739, 1240
AjiI	CACGTC	1 cut(s)	1834
AjnI	CCWGG	5 cut(s)	134, 614, 762, 939, 1918
AloI	GAACNNNNNNTCC	2 cut(s)	890, 922
Alw21I	GWGCWC	5 cut(s)	339, 549, 789, 1167, 1449
Alw26I	GTCTC	5 cut(s)	457, 676, 1395, 1825, 1897
AlwI	GGATC	6 cut(s)	64, 404, 613, 626, 753, 890
AlwNI	CAGNNNCTG	1 cut(s)	1699
Ama87I	CYCGRG	1 cut(s)	174
ApaI	GGGCCC	1 cut(s)	215
ApeKI	GCWGC	5 cut(s)	16, 590, 1567, 1575, 2037
ArsI	GACNNNNNNTTYG	2 cut(s)	982, 1014
AseI	ATTAAT	1 cut(s)	1988
AspS9I	GGNCC	9 cut(s)	211, 212, 473, 682, 902, 937, 1458, 1477, 2261
AsuII	TTCGAA	1 cut(s)	1327
AvaI	CYCGRG	1 cut(s)	174
AvaII	GGWCC	5 cut(s)	682, 902, 937, 1458, 1477
BaeGI	GKGCMC	1 cut(s)	215
BamHI	GGATCC	1 cut(s)	618
BanI	GGYRCC	7 cut(s)	4, 425, 695, 1105, 1397, 1706, 1808
BanII	GRGCYC	9 cut(s)	215, 306, 339, 549, 789, 987, 1167, 1279, 1449
BarI	GAAGNNNNNNTAC	2 cut(s)	773, 805
BauI	CACGAG	1 cut(s)	1625
BbsI	GAAGAC	3 cut(s)	712, 1420, 1970
Bbv12I	GWGCWC	5 cut(s)	339, 549, 789, 1167, 1449
BbvI	GCAGC	5 cut(s)	3, 577, 1579, 1587, 2049
BceAI	ACGGC	5 cut(s)	508, 581, 701, 1720, 1860
BciT130I	CCWGG	5 cut(s)	136, 616, 764, 941, 1920
BciVI	GTATCC	2 cut(s)	843, 985
BclI	TGATCA	1 cut(s)	1371
BcoDI	GTCTC	5 cut(s)	457, 676, 1395, 1825, 1897
BcuI	ACTAGT	2 cut(s)	739, 1240
BfaI	CTAG	5 cut(s)	626, 740, 812, 1241, 1787
BfmI	CTRYAG	1 cut(s)	2337
BfoI	RGCGCY	2 cut(s)	351, 1130
BfuAI	ACCTGC	1 cut(s)	16
BfuI	GTATCC	2 cut(s)	843, 985
BglI	GCCNNNNNGGC	1 cut(s)	363
BlpI	GCTNAGC	3 cut(s)	543, 1571, 1911
Bme1390I	CCNGG	5 cut(s)	136, 616, 764, 941, 1920
Bme18I	GGWCC	5 cut(s)	682, 902, 937, 1458, 1477
BmeRI	GACNNNNNGTC	1 cut(s)	1421
BmeT110I	CYCGRG	1 cut(s)	174
BmgBI	CACGTC	1 cut(s)	1834
BmgT120I	GGNCC	9 cut(s)	211, 212, 473, 682, 902, 937, 1458, 1477, 2261
BmrFI	CCNGG	5 cut(s)	136, 616, 764, 941, 1920
BmrI	ACTGGG	1 cut(s)	515
BmsI	GCATC	7 cut(s)	390, 429, 543, 585, 1699, 1924, 2323
BmuI	ACTGGG	1 cut(s)	515
BpiI	GAAGAC	3 cut(s)	712, 1420, 1970
BplI	GAGNNNNNCTC	2 cut(s)	1445, 1477
Bpu1102I	GCTNAGC	3 cut(s)	543, 1571, 1911
Bpu14I	TTCGAA	1 cut(s)	1327
BpuEI	CTTGAG	3 cut(s)	353, 533, 971
BsaHI	GRCGYC	3 cut(s)	495, 567, 606
BsaJI	CCNNGG	9 cut(s)	376, 415, 762, 822, 1122, 1473, 1659, 1710, 1919
BsaXI	ACNNNNNCTCC	7 cut(s)	90, 120, 644, 674, 890, 920, 2378
Bsc4I	CCNNNNNNNGG	7 cut(s)	225, 266, 300, 393, 899, 911, 946
Bse118I	RCCGGY	2 cut(s)	19, 647
Bse1I	ACTGG	4 cut(s)	521, 978, 1893, 1911
Bse3DI	GCAATG	1 cut(s)	1671
BseBI	CCWGG	5 cut(s)	136, 616, 764, 941, 1920
BseDI	CCNNGG	9 cut(s)	376, 415, 762, 822, 1122, 1473, 1659, 1710, 1919
BseGI	GGATG	7 cut(s)	102, 206, 351, 534, 726, 1359, 1915
BseLI	CCNNNNNNNGG	7 cut(s)	225, 266, 300, 393, 899, 911, 946
BseMI	GCAATG	1 cut(s)	1671
BseMII	CTCAG	5 cut(s)	534, 1585, 1836, 1902, 2171
BseNI	ACTGG	4 cut(s)	521, 978, 1893, 1911
BsePI	GCGCGC	1 cut(s)	1644
BseRI	GAGGAG	5 cut(s)	244, 545, 677, 2087, 2144
BseSI	GKGCMC	1 cut(s)	215
BseX3I	CGGCCG	2 cut(s)	571, 2348
BseXI	GCAGC	5 cut(s)	3, 577, 1579, 1587, 2049
BseYI	CCCAGC	1 cut(s)	208
Bsh1236I	CGCG	9 cut(s)	444, 824, 857, 869, 1395, 1410, 1646, 2162, 2347
Bsh1285I	CGRYCG	2 cut(s)	574, 2351
BshNI	GGYRCC	7 cut(s)	4, 425, 695, 1105, 1397, 1706, 1808
BsiEI	CGRYCG	2 cut(s)	574, 2351
BsiHKAI	GWGCWC	5 cut(s)	339, 549, 789, 1167, 1449
BsiHKCI	CYCGRG	1 cut(s)	174
BsiSI	CCGG	5 cut(s)	20, 166, 648, 699, 2384
BslFI	GGGAC	4 cut(s)	358, 1090, 1737, 2210
BslI	CCNNNNNNNGG	7 cut(s)	225, 266, 300, 393, 899, 911, 946
BsmAI	GTCTC	5 cut(s)	457, 676, 1395, 1825, 1897
BsmBI	CGTCTC	3 cut(s)	457, 676, 1395
BsmFI	GGGAC	4 cut(s)	358, 1090, 1737, 2210
BsmI	GAATGC	1 cut(s)	221
BsoBI	CYCGRG	1 cut(s)	174
Bsp119I	TTCGAA	1 cut(s)	1327
Bsp120I	GGGCCC	1 cut(s)	211
Bsp1286I	GDGCHC	9 cut(s)	215, 306, 339, 549, 789, 987, 1167, 1279, 1449
Bsp1407I	TGTACA	1 cut(s)	1254
Bsp143I	GATC	9 cut(s)	56, 396, 618, 758, 882, 1068, 1371, 1876, 2157
Bsp1720I	GCTNAGC	3 cut(s)	543, 1571, 1911
Bsp19I	CCATGG	2 cut(s)	1473, 1710
BspCNI	CTCAG	5 cut(s)	535, 1584, 1835, 1903, 2172
BspFNI	CGCG	9 cut(s)	444, 824, 857, 869, 1395, 1410, 1646, 2162, 2347
BspHI	TCATGA	1 cut(s)	744
BspMI	ACCTGC	1 cut(s)	16
BspPI	GGATC	6 cut(s)	64, 404, 613, 626, 753, 890
BspQI	GCTCTTC	1 cut(s)	96
BspT104I	TTCGAA	1 cut(s)	1327
BspT107I	GGYRCC	7 cut(s)	4, 425, 695, 1105, 1397, 1706, 1808
BsrBI	CCGCTC	2 cut(s)	667, 1456
BsrDI	GCAATG	1 cut(s)	1671
BsrFI	RCCGGY	2 cut(s)	19, 647
BsrGI	TGTACA	1 cut(s)	1254
BsrI	ACTGG	4 cut(s)	521, 978, 1893, 1911
BssAI	RCCGGY	2 cut(s)	19, 647
BssECI	CCNNGG	9 cut(s)	376, 415, 762, 822, 1122, 1473, 1659, 1710, 1919
BssHII	GCGCGC	1 cut(s)	1644
BssMI	GATC	9 cut(s)	56, 396, 618, 758, 882, 1068, 1371, 1876, 2157
BssNI	GRCGYC	3 cut(s)	495, 567, 606
BssSI	CACGAG	1 cut(s)	1625
BssT1I	CCWWGG	3 cut(s)	1122, 1473, 1710
Bst2BI	CACGAG	1 cut(s)	1625
Bst2UI	CCWGG	5 cut(s)	136, 616, 764, 941, 1920
Bst6I	CTCTTC	8 cut(s)	96, 323, 1514, 1612, 1864, 2046, 2106, 2302
BstACI	GRCGYC	3 cut(s)	495, 567, 606
BstAUI	TGTACA	1 cut(s)	1254
BstBI	TTCGAA	1 cut(s)	1327
BstC8I	GCNNGC	9 cut(s)	21, 532, 1339, 1489, 1593, 1642, 1646, 1828, 2186
BstDEI	CTNAG	6 cut(s)	543, 1571, 1822, 1911, 2180, 2278
BstDSI	CCRYGG	4 cut(s)	822, 1473, 1659, 1710
BstF5I	GGATG	7 cut(s)	102, 206, 351, 534, 726, 1359, 1915
BstFNI	CGCG	9 cut(s)	444, 824, 857, 869, 1395, 1410, 1646, 2162, 2347
BstH2I	RGCGCY	2 cut(s)	351, 1130
BstKTI	GATC	9 cut(s)	59, 399, 621, 761, 885, 1071, 1374, 1879, 2160
BstMAI	GTCTC	5 cut(s)	457, 676, 1395, 1825, 1897
BstMBI	GATC	9 cut(s)	56, 396, 618, 758, 882, 1068, 1371, 1876, 2157
BstMCI	CGRYCG	2 cut(s)	574, 2351
BstNI	CCWGG	5 cut(s)	136, 616, 764, 941, 1920
BstNSI	RCATGY	3 cut(s)	1341, 1624, 1766
BstSCI	CCNGG	5 cut(s)	134, 614, 762, 939, 1918
BstSFI	CTRYAG	1 cut(s)	2337
BstSLI	GKGCMC	1 cut(s)	215
BstUI	CGCG	9 cut(s)	444, 824, 857, 869, 1395, 1410, 1646, 2162, 2347
BstV1I	GCAGC	5 cut(s)	3, 577, 1579, 1587, 2049
BstV2I	GAAGAC	3 cut(s)	712, 1420, 1970
BstX2I	RGATCY	2 cut(s)	618, 758
BstXI	CCANNNNNNTGG	1 cut(s)	1474
BstYI	RGATCY	2 cut(s)	618, 758
BstZI	CGGCCG	2 cut(s)	571, 2348
BsuI	GTATCC	2 cut(s)	843, 985
BtgI	CCRYGG	4 cut(s)	822, 1473, 1659, 1710
BtgZI	GCGATG	3 cut(s)	367, 562, 622
BtrI	CACGTC	1 cut(s)	1834
BtsCI	GGATG	7 cut(s)	102, 206, 351, 534, 726, 1359, 1915
BtsI	GCAGTG	1 cut(s)	1257
BtsIMutI	CAGTG	4 cut(s)	985, 1257, 1725, 1886
BveI	ACCTGC	1 cut(s)	16
Cac8I	GCNNGC	9 cut(s)	21, 532, 1339, 1489, 1593, 1642, 1646, 1828, 2186
CaiI	CAGNNNCTG	1 cut(s)	1699
CciI	TCATGA	1 cut(s)	744
CciNI	GCGGCCGC	1 cut(s)	2348
Cfr10I	RCCGGY	2 cut(s)	19, 647
Cfr13I	GGNCC	9 cut(s)	211, 212, 473, 682, 902, 937, 1458, 1477, 2261
Cfr42I	CCGCGG	1 cut(s)	825
CseI	GACGC	5 cut(s)	484, 787, 1044, 1567, 2331
CsiI	ACCWGGT	2 cut(s)	134, 939
Csp6I	GTAC	3 cut(s)	1172, 1255, 2077
CviQI	GTAC	3 cut(s)	1172, 1255, 2077
DdeI	CTNAG	6 cut(s)	543, 1571, 1822, 1911, 2180, 2278
DpnI	GATC	9 cut(s)	58, 398, 620, 760, 884, 1070, 1373, 1878, 2159
DpnII	GATC	9 cut(s)	56, 396, 618, 758, 882, 1068, 1371, 1876, 2157
DraI	TTTAAA	1 cut(s)	1614
DriI	GACNNNNNGTC	1 cut(s)	1421
EaeI	YGGCCR	4 cut(s)	571, 819, 1589, 2348
EagI	CGGCCG	2 cut(s)	571, 2348
Eam1104I	CTCTTC	8 cut(s)	96, 323, 1514, 1612, 1864, 2046, 2106, 2302
Eam1105I	GACNNNNNGTC	1 cut(s)	1421
EarI	CTCTTC	8 cut(s)	96, 323, 1514, 1612, 1864, 2046, 2106, 2302
EciI	GGCGGA	3 cut(s)	213, 344, 719
Ecl136II	GAGCTC	5 cut(s)	337, 547, 787, 1165, 1447
EclXI	CGGCCG	2 cut(s)	571, 2348
Eco130I	CCWWGG	3 cut(s)	1122, 1473, 1710
Eco147I	AGGCCT	1 cut(s)	1559
Eco24I	GRGCYC	9 cut(s)	215, 306, 339, 549, 789, 987, 1167, 1279, 1449
Eco47I	GGWCC	5 cut(s)	682, 902, 937, 1458, 1477
Eco52I	CGGCCG	2 cut(s)	571, 2348
Eco53kI	GAGCTC	5 cut(s)	337, 547, 787, 1165, 1447
Eco57I	CTGAAG	2 cut(s)	453, 2292
Eco88I	CYCGRG	1 cut(s)	174
EcoICRI	GAGCTC	5 cut(s)	337, 547, 787, 1165, 1447
EcoO109I	RGGNCCY	4 cut(s)	212, 473, 682, 937
EcoRII	CCWGG	5 cut(s)	134, 614, 762, 939, 1918
EcoT14I	CCWWGG	3 cut(s)	1122, 1473, 1710
EcoT38I	GRGCYC	9 cut(s)	215, 306, 339, 549, 789, 987, 1167, 1279, 1449
ErhI	CCWWGG	3 cut(s)	1122, 1473, 1710
Esp3I	CGTCTC	3 cut(s)	457, 676, 1395
FalI	AAGNNNNNCTT	2 cut(s)	2177, 2209
FaqI	GGGAC	4 cut(s)	358, 1090, 1737, 2210
FauI	CCCGC	9 cut(s)	39, 267, 386, 539, 672, 817, 1004, 1361, 1496
FauNDI	CATATG	1 cut(s)	1634
FbaI	TGATCA	1 cut(s)	1371
FblI	GTMKAC	1 cut(s)	1425
FokI	GGATG	7 cut(s)	89, 213, 338, 521, 713, 1346, 1902
FriOI	GRGCYC	9 cut(s)	215, 306, 339, 549, 789, 987, 1167, 1279, 1449
FspBI	CTAG	5 cut(s)	626, 740, 812, 1241, 1787
GsaI	CCCAGC	1 cut(s)	212
HaeII	RGCGCY	2 cut(s)	351, 1130
HapII	CCGG	5 cut(s)	20, 166, 648, 699, 2384
HgaI	GACGC	5 cut(s)	484, 787, 1044, 1567, 2331
Hin1I	GRCGYC	3 cut(s)	495, 567, 606
HincII	GTYRAC	3 cut(s)	601, 1932, 2371
HindII	GTYRAC	3 cut(s)	601, 1932, 2371
HindIII	AAGCTT	1 cut(s)	2272
HpaII	CCGG	5 cut(s)	20, 166, 648, 699, 2384
Hpy166II	GTNNAC	9 cut(s)	132, 601, 1218, 1255, 1426, 1744, 1932, 2143, 2371
Hpy188I	TCNGA	7 cut(s)	433, 1453, 1695, 1865, 2181, 2270, 2290
Hpy188III	TCNNGA	8 cut(s)	226, 454, 745, 832, 988, 1075, 1582, 1880
Hpy8I	GTNNAC	9 cut(s)	132, 601, 1218, 1255, 1426, 1744, 1932, 2143, 2371
HpyCH4IV	ACGT	3 cut(s)	567, 606, 1833
HpyCH4V	TGCA	3 cut(s)	1050, 1435, 1624
HpyF3I	CTNAG	6 cut(s)	543, 1571, 1822, 1911, 2180, 2278
HpySE526I	ACGT	3 cut(s)	567, 606, 1833
Hsp92I	GRCGYC	3 cut(s)	495, 567, 606
KroI	GCCGGC	1 cut(s)	19
KroNI	GCCGGC	1 cut(s)	21
Ksp22I	TGATCA	1 cut(s)	1371
KspI	CCGCGG	1 cut(s)	825
Kzo9I	GATC	9 cut(s)	56, 396, 618, 758, 882, 1068, 1371, 1876, 2157
LguI	GCTCTTC	1 cut(s)	96
Lsp1109I	GCAGC	5 cut(s)	3, 577, 1579, 1587, 2049
LweI	GCATC	7 cut(s)	390, 429, 543, 585, 1699, 1924, 2323
MabI	ACCWGGT	2 cut(s)	134, 939
MaeI	CTAG	5 cut(s)	626, 740, 812, 1241, 1787
MaeII	ACGT	3 cut(s)	567, 606, 1833
MaeIII	GTNAC	4 cut(s)	799, 832, 1081, 2243
MalI	GATC	9 cut(s)	58, 398, 620, 760, 884, 1070, 1373, 1878, 2159
MbiI	CCGCTC	2 cut(s)	667, 1456
MboI	GATC	9 cut(s)	56, 396, 618, 758, 882, 1068, 1371, 1876, 2157
MflI	RGATCY	2 cut(s)	618, 758
MhlI	GDGCHC	9 cut(s)	215, 306, 339, 549, 789, 987, 1167, 1279, 1449
MluCI	AATT	3 cut(s)	994, 1985, 1989
MlyI	GAGTC	9 cut(s)	186, 1394, 1410, 1724, 1794, 2123, 2170, 2246, 2396
MroNI	GCCGGC	1 cut(s)	19
MseI	TTAA	4 cut(s)	1613, 1988, 2171, 2404
MslI	CAYNNNNRTG	3 cut(s)	275, 581, 1472
MspA1I	CMGCKG	3 cut(s)	16, 590, 824
MspI	CCGG	5 cut(s)	20, 166, 648, 699, 2384
MspR9I	CCNGG	5 cut(s)	136, 616, 764, 941, 1920
Mva1269I	GAATGC	1 cut(s)	221
MvaI	CCWGG	5 cut(s)	136, 616, 764, 941, 1920
MvnI	CGCG	9 cut(s)	444, 824, 857, 869, 1395, 1410, 1646, 2162, 2347
NaeI	GCCGGC	1 cut(s)	21
NcoI	CCATGG	2 cut(s)	1473, 1710
NdeI	CATATG	1 cut(s)	1634
NdeII	GATC	9 cut(s)	56, 396, 618, 758, 882, 1068, 1371, 1876, 2157
NgoMIV	GCCGGC	1 cut(s)	19
NmeAIII	GCCGAG	2 cut(s)	357, 396
NmuCI	GTSAC	4 cut(s)	799, 832, 1081, 2243
NotI	GCGGCCGC	1 cut(s)	2348
NspI	RCATGY	3 cut(s)	1341, 1624, 1766
NspV	TTCGAA	1 cut(s)	1327
PaeI	GCATGC	1 cut(s)	1341
PagI	TCATGA	1 cut(s)	744
PaqCI	CACCTGC	1 cut(s)	16
PauI	GCGCGC	1 cut(s)	1644
PceI	AGGCCT	1 cut(s)	1559
PciSI	GCTCTTC	1 cut(s)	96
PcsI	WCGNNNNNNNCGW	3 cut(s)	714, 1560, 1623
PctI	GAATGC	1 cut(s)	221
PdiI	GCCGGC	1 cut(s)	21
PfeI	GAWTC	5 cut(s)	702, 1585, 1783, 1944, 2320
PleI	GAGTC	9 cut(s)	185, 1393, 1410, 1723, 1794, 2122, 2170, 2245, 2395
PpsI	GAGTC	9 cut(s)	185, 1393, 1410, 1723, 1794, 2122, 2170, 2245, 2395
PpuMI	RGGWCCY	2 cut(s)	682, 937
PshBI	ATTAAT	1 cut(s)	1988
Psp124BI	GAGCTC	5 cut(s)	339, 549, 789, 1167, 1449
Psp5II	RGGWCCY	2 cut(s)	682, 937
Psp6I	CCWGG	5 cut(s)	134, 614, 762, 939, 1918
PspFI	CCCAGC	1 cut(s)	208
PspGI	CCWGG	5 cut(s)	134, 614, 762, 939, 1918
PspOMI	GGGCCC	1 cut(s)	211
PspPI	GGNCC	9 cut(s)	211, 212, 473, 682, 902, 937, 1458, 1477, 2261
PspPPI	RGGWCCY	2 cut(s)	682, 937
PstNI	CAGNNNCTG	1 cut(s)	1699
PsuI	RGATCY	2 cut(s)	618, 758
PteI	GCGCGC	1 cut(s)	1644
RsaI	GTAC	3 cut(s)	1173, 1256, 2078
RsaNI	GTAC	3 cut(s)	1172, 1255, 2077
RseI	CAYNNNNRTG	3 cut(s)	275, 581, 1472
SacI	GAGCTC	5 cut(s)	339, 549, 789, 1167, 1449
SacII	CCGCGG	1 cut(s)	825
SapI	GCTCTTC	1 cut(s)	96
SaqAI	TTAA	4 cut(s)	1613, 1988, 2171, 2404
Sau3AI	GATC	9 cut(s)	56, 396, 618, 758, 882, 1068, 1371, 1876, 2157
Sau96I	GGNCC	9 cut(s)	211, 212, 473, 682, 902, 937, 1458, 1477, 2261
SchI	GAGTC	9 cut(s)	186, 1394, 1410, 1724, 1794, 2123, 2170, 2246, 2396
ScrFI	CCNGG	5 cut(s)	136, 616, 764, 941, 1920
SduI	GDGCHC	9 cut(s)	215, 306, 339, 549, 789, 987, 1167, 1279, 1449
SexAI	ACCWGGT	2 cut(s)	134, 939
SfaNI	GCATC	7 cut(s)	390, 429, 543, 585, 1699, 1924, 2323
SfcI	CTRYAG	1 cut(s)	2337
Sfr303I	CCGCGG	1 cut(s)	825
SfuI	TTCGAA	1 cut(s)	1327
SgrBI	CCGCGG	1 cut(s)	825
SinI	GGWCC	5 cut(s)	682, 902, 937, 1458, 1477
SmiMI	CAYNNNNRTG	3 cut(s)	275, 581, 1472
SmlI	CTYRAG	3 cut(s)	332, 548, 986
SmoI	CTYRAG	3 cut(s)	332, 548, 986
SpeI	ACTAGT	2 cut(s)	739, 1240
SphI	GCATGC	1 cut(s)	1341
Sse9I	AATT	3 cut(s)	994, 1985, 1989
SseBI	AGGCCT	1 cut(s)	1559
SspI	AATATT	1 cut(s)	1332
SspMI	CTAG	5 cut(s)	626, 740, 812, 1241, 1787
SstI	GAGCTC	5 cut(s)	339, 549, 789, 1167, 1449
StuI	AGGCCT	1 cut(s)	1559
StyD4I	CCNGG	5 cut(s)	134, 614, 762, 939, 1918
StyI	CCWWGG	3 cut(s)	1122, 1473, 1710
TaiI	ACGT	3 cut(s)	570, 609, 1836
TaqI	TCGA	8 cut(s)	339, 455, 1091, 1327, 1383, 1554, 1759, 2240
TasI	AATT	3 cut(s)	994, 1985, 1989
TatI	WGTACW	2 cut(s)	1254, 2076
TfiI	GAWTC	5 cut(s)	702, 1585, 1783, 1944, 2320
Tru1I	TTAA	4 cut(s)	1613, 1988, 2171, 2404
Tru9I	TTAA	4 cut(s)	1613, 1988, 2171, 2404
TscAI	CASTG	4 cut(s)	985, 1257, 1725, 1893
TseFI	GTSAC	4 cut(s)	799, 832, 1081, 2243
TseI	GCWGC	5 cut(s)	16, 590, 1567, 1575, 2037
Tsp45I	GTSAC	4 cut(s)	799, 832, 1081, 2243
TspDTI	ATGAA	5 cut(s)	53, 1157, 1194, 1216, 2064
TspGWI	ACGGA	3 cut(s)	664, 851, 894
TspRI	CASTG	4 cut(s)	985, 1257, 1725, 1893
VpaK11BI	GGWCC	5 cut(s)	682, 902, 937, 1458, 1477
VspI	ATTAAT	1 cut(s)	1988
XceI	RCATGY	3 cut(s)	1341, 1624, 1766
XcmI	CCANNNNNNNNNTGG	2 cut(s)	1438, 1717
XmiI	GTMKAC	1 cut(s)	1425
XspI	CTAG	5 cut(s)	626, 740, 812, 1241, 1787
ZraI	GACGTC	2 cut(s)	568, 607

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.