Basic Information

Type: gene

Biological Identity

Species

rosa_samantha

Chromosome

Chr3B

Orthogroup ID

OG0004570

Physical Location & Seq

Genomic Coordinates Reverse (-)

29434770 .. 29438878

Gene Structure 4109 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

Rh3BG293400.1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2163 bp

ATGACGGTGCTAGACACGGTAGCCAACATCGGGCTCTGCTTCTTCCTCTTCCTCGTCGGCCTTGAGCTTGACATCAGCGCCATCCGCCGCACAGGCAAGAAGTCCCTCGGCATCGCCTTCTGTGGGATCACCCTCCCCTTCGTCCTCGGCATCGGCACCTCTGAAGCCCTACGCGCCACTGTCTCCAAAGGCGTGAAACAAGGCCCCTTCCTCGTCTTCAACGGTGTTGCCCTCTCCATCACCGCCTTCCCCGTCCTCGCCCGCATCCTCGCTGAGCTCAAGCTCCTCACCACCGATGTCGGCCGCATCGCCATGTCCGCCGCCGCCGTCAACGACGTCGCCGCCTGGATCCTTCTAGCCCTCGCCATCGCCCTCACCGGCAACAACACCTCACCGCTCGTCTCCGTCTGGGTCCTCCTCTGCGGTGCTGGGTTCGTTGGGTTCGCCGTCGTCGCCATTCGCCCCATACTGGTCATGATGGCGCGAAGGTCCCCGGAGGGCGAACCTGTGAAGGAGCTCTACATCTGCATCACTCTCTCAGTGGTTTTGGCCGCGGGGTTCGTGACTGACACGATTGGGATACACGCATTGTTTGGCGCGTTCGTGGTGGGGATCATAATGCCGAAAGAGGGTCCGTTCGCGGGTGTGTTGATAGAGAAGATTGAGGACCTGGTTTCTGGGCTTCTATTGCCGCTTTATTTTGTATCCAGTGGGCTCAAGACTAATTTTTTGACTATAAGCGGGGGGGTGTCGTGGGGTATGCTGGTGTTGGTGATATGTACAGCGTCGTTTGGGAAGATTGTCGGGACTGTCACCGTGTCGATGCTGTTCAAGGTGCCTTTCAGGGAAGCCTTGGCGCTTGGCTTCCTTATGAATACAAAGGGCTTGGTGGAGCTCATCGTCCTCAATATTGGCAAGGATAGAAAGGTACTGAACGAACAGACTTTTGCCATCTTTATTTTGATGGCGTTGTTCACCACCTTCATCACCACACCACTAGTGATGGCAGTGTACAAACCAGCAAGAAAGGGAGCCCCTTACAAACTACGTACCATTTTCCGCAAAAACCCAGACACAGAACTTCGAATATTGGCATGCTTCCACACCACCCGCAACATCCCCACCATGATCAACCTCATCGAGTCGTCTCGCGGCACCCACAAGCGCGGACGACTCACCGTCTACGCCATGCACCTCATGGAGCTCTCGGAGCGGTCCTCTGCAATCTCCATGGTCCACAAAGCCCGCTATAATGGCCTCCCATTTTGGAACAAGAAGAGTGATGGAAATGACCAAATGGTTATTGCTTTTGAGGCCTACGAGCAGCTCAGCAGCGTCAAGATTCGGCCAGCCACTGCCATCTCTTCTTTAAATGACATGCACGAGGACATATGCGCCTGCGCGCACCAAAAAACCGTGGCAATGATTTTATTGCCATTTCACAAGCATCAGAAGCTGGACGGCACCATGGAGTCGGTGGGACACTCATTTCAAGTGGTGAACGAGCGTGTGCTCCGACATGCGCCTTGTTCGGTTGGGATTCTAGTTGATAGAGGACTAGGTGGCACCACGCAGGTCTCAGCCAGCGAAGTGTCGTATACGGCGGTGGTGGCGTTCTTCGGAGGAAGAGATGACCGGGAGGCACTGGCTTATGGTATGAGATTGGCTGAACATCCTGGGATTGCGTTGACTGTTTTTAGATTCGTTGCTCCACAAGGGAAGACATTGAGATTTGGAGCGAAATTAGTTGGCATAACAGCTGACAACAAGAAAATACTGAAAGAAGAAGATAATAGCCAAGAGAATGAAGAGGACGAGAAGTTCTTGGAGGAGTACAAGAATGTAAGAAGTAGCAGTAAGGAAAAGGAAGAGTCCATGTTTTACGAGGAGAAGGTGGTGGACAGCAAAGGGGAGATCGCGGTGGCGTTAAAGACTCTGAGCAAGCACAACTTGTTTATAGTAGGGAGAATGTCGCCGACTGTCCCGTTGGTAGAGTTGAGTGACTGTGCGGAGCTTGGGCCAATCGGAAGCTTCTTAGCCTCGTCAGACTTCTCTTCCACAGCTTCAGTTGTGGTGATTCAACAGTATGATGCTACAGCGTCGCGGCCGCTTGTGGTGGAGGAAGCTGACTATGAGTTGCCGGAGTCATCATCTATGGTTTAA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005768 GO:0005770 GO:0006810 GO:0006811 GO:0006812 GO:0006885 GO:0008150 GO:0012505 GO:0015672 GO:0031410 GO:0031982 GO:0042592 GO:0043226 GO:0043227 GO:0043229 GO:0044424 GO:0044444 GO:0044464 GO:0048878 GO:0050801 GO:0051179 GO:0051234 GO:0055067 GO:0055080 GO:0065007 GO:0065008 GO:0097708 GO:0098771

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

Na_H_Exchanger

Protein Analysis

720

Amino Acids

78.2

Weight (kDa)

8.19

Isoelectric Point (pI)

39.21

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Na_H_Exchanger	PF00999	3 - 334	7.6e-53	Sodium/hydrogen exchanger, transmembrane
CHX17_2nd	PF23256	389 - 529	2.5e-51	Plant cation/H(+) antiporter domain
CHX17_C	PF23259	535 - 697	1.8e-18	Plant cation/H(+) antiporter, C-terminal domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0004570)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT3G17630 AT3G17630
fragaria_vesca	FvH4_4g21880
malus_domestica	MD13G1136000.v1.1 MD16G1131400.v1.1
prunus_persica	Prupe.1G209400_v2.0.a1
pyrus_communis	pycom16g11580
rosa_chinensis	RchiOBHm_Chr3g0483861
rosa_laevigata	RLG00000007178 RLG00000023257
rosa_multiflora	Rmu_co8183874.1_g000001 Rmu_co8462775.1_g000001 Rmu_sc0004940.1_g000012 Rmu_sc0005901.1_g000001 Rmu_sc0009599.1_g000012
rosa_roxburghii	Rroxscaffold_5G00369850 Rroxscaffold_6G00398090
rosa_rugosa	Rorug03G0208200.1 Rorug03G0208300 Rorug04G0222700 Rorug04G0222800
rosa_samantha	Rh3AG257000 Rh3BG293400 Rh3CG291700 Rh3DG286700 Rh4AG279900 Rh4AG280100 Rh4BG285300 Rh4BG285500 Rh4CG300900
rosa_wichuraiana	Rw3G023230

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AatII	GACGTC	1 cut(s)	339
Acc36I	ACCTGC	1 cut(s)	1564
AccB1I	GGYRCC	5 cut(s)	155, 835, 1154, 1463, 1565
AccBSI	CCGCTC	2 cut(s)	397, 1213
AccI	GTMKAC	2 cut(s)	1182, 1598
AclWI	GGATC	4 cut(s)	134, 343, 356, 620
AcoI	YGGCCR	4 cut(s)	301, 549, 1346, 2105
AcuI	CTGAAG	2 cut(s)	183, 2049
AcyI	GRCGYC	1 cut(s)	336
AfaI	GTAC	5 cut(s)	781, 930, 1013, 1051, 1835
AfiI	CCNNNNNNNGG	5 cut(s)	30, 123, 469, 629, 641
AgsI	TTSAA	4 cut(s)	220, 832, 1493, 2081
AhdI	GACNNNNNGTC	1 cut(s)	1178
AhlI	ACTAGT	1 cut(s)	997
AjnI	CCWGG	3 cut(s)	344, 669, 1675
AloI	GAACNNNNNNTCC	2 cut(s)	1805, 1837
Alw21I	GWGCWC	5 cut(s)	279, 519, 897, 1206, 1515
Alw26I	GTCTC	4 cut(s)	187, 406, 1152, 1582
AlwI	GGATC	4 cut(s)	134, 343, 356, 620
AlwNI	CAGNNNCTG	2 cut(s)	1355, 1456
AoxI	GGCC	9 cut(s)	58, 202, 301, 549, 1255, 1314, 1346, 2018, 2105
ApeKI	GCWGC	2 cut(s)	1324, 1332
ArsI	GACNNNNNNTTYG	2 cut(s)	712, 744
AspS9I	GGNCC	8 cut(s)	203, 412, 489, 632, 667, 1215, 1234, 2018
AsuC2I	CCSGG	2 cut(s)	494, 1637
AsuII	TTCGAA	1 cut(s)	1084
AvaII	GGWCC	6 cut(s)	412, 489, 632, 667, 1215, 1234
BamHI	GGATCC	1 cut(s)	348
BanI	GGYRCC	5 cut(s)	155, 835, 1154, 1463, 1565
BanII	GRGCYC	7 cut(s)	36, 279, 519, 717, 897, 1036, 1206
BarI	GAAGNNNNNNTAC	2 cut(s)	503, 535
BauI	CACGAG	1 cut(s)	1382
BbsI	GAAGAC	2 cut(s)	208, 1727
Bbv12I	GWGCWC	5 cut(s)	279, 519, 897, 1206, 1515
BbvI	GCAGC	2 cut(s)	1336, 1344
BccI	CCATC	9 cut(s)	89, 245, 374, 472, 958, 959, 997, 1277, 1367
BceAI	ACGGC	4 cut(s)	311, 431, 1477, 1617
BciT130I	CCWGG	3 cut(s)	346, 671, 1677
BciVI	GTATCC	2 cut(s)	573, 715
BclI	TGATCA	1 cut(s)	1128
BcnI	CCSGG	2 cut(s)	494, 1637
BcoDI	GTCTC	4 cut(s)	187, 406, 1152, 1582
BcuI	ACTAGT	1 cut(s)	997
BfaI	CTAG	5 cut(s)	11, 356, 998, 1544, 1559
BfmI	CTRYAG	1 cut(s)	2094
BfoI	RGCGCY	2 cut(s)	81, 860
BfuAI	ACCTGC	1 cut(s)	1564
BfuI	GTATCC	2 cut(s)	573, 715
BglI	GCCNNNNNGGC	1 cut(s)	93
BlpI	GCTNAGC	2 cut(s)	273, 1328
Bme1390I	CCNGG	5 cut(s)	346, 494, 671, 1637, 1677
Bme18I	GGWCC	6 cut(s)	412, 489, 632, 667, 1215, 1234
BmeRI	GACNNNNNGTC	1 cut(s)	1178
BmgT120I	GGNCC	8 cut(s)	203, 412, 489, 632, 667, 1215, 1234, 2018
BmrFI	CCNGG	5 cut(s)	346, 494, 671, 1637, 1677
BmsI	GCATC	8 cut(s)	120, 159, 273, 315, 537, 813, 1456, 2080
BpiI	GAAGAC	2 cut(s)	208, 1727
BplI	GAGNNNNNCTC	2 cut(s)	1202, 1234
Bpu1102I	GCTNAGC	2 cut(s)	273, 1328
Bpu14I	TTCGAA	1 cut(s)	1084
BpuEI	CTTGAG	3 cut(s)	83, 263, 701
BpuMI	CCSGG	2 cut(s)	494, 1637
BsaAI	YACGTR	1 cut(s)	1049
BsaHI	GRCGYC	1 cut(s)	336
BsaI	GGTCTC	1 cut(s)	1582
BsaJI	CCNNGG	9 cut(s)	106, 145, 492, 552, 852, 1230, 1416, 1467, 1676
Bsc4I	CCNNNNNNNGG	5 cut(s)	30, 123, 469, 629, 641
Bse118I	RCCGGY	1 cut(s)	377
Bse1I	ACTGG	3 cut(s)	474, 708, 1650
Bse3DI	GCAATG	1 cut(s)	1428
BseBI	CCWGG	3 cut(s)	346, 671, 1677
BseDI	CCNNGG	9 cut(s)	106, 145, 492, 552, 852, 1230, 1416, 1467, 1676
BseGI	GGATG	4 cut(s)	81, 264, 1116, 1672
BseLI	CCNNNNNNNGG	5 cut(s)	30, 123, 469, 629, 641
BseMI	GCAATG	1 cut(s)	1428
BseMII	CTCAG	5 cut(s)	264, 552, 1342, 1593, 1928
BseNI	ACTGG	3 cut(s)	474, 708, 1650
BsePI	GCGCGC	1 cut(s)	1401
BseRI	GAGGAG	4 cut(s)	275, 407, 1844, 1901
BseX3I	CGGCCG	2 cut(s)	301, 2105
BseXI	GCAGC	2 cut(s)	1336, 1344
BseYI	CCCAGC	1 cut(s)	428
Bsh1285I	CGRYCG	2 cut(s)	304, 2108
BshFI	GGCC	9 cut(s)	60, 204, 303, 551, 1257, 1316, 1348, 2020, 2107
BshNI	GGYRCC	5 cut(s)	155, 835, 1154, 1463, 1565
BsiEI	CGRYCG	2 cut(s)	304, 2108
BsiHKAI	GWGCWC	5 cut(s)	279, 519, 897, 1206, 1515
BsiSI	CCGG	4 cut(s)	378, 494, 1636, 2141
BslFI	GGGAC	5 cut(s)	88, 475, 820, 1494, 1967
BslI	CCNNNNNNNGG	5 cut(s)	30, 123, 469, 629, 641
BsmAI	GTCTC	4 cut(s)	187, 406, 1152, 1582
BsmBI	CGTCTC	2 cut(s)	406, 1152
BsmFI	GGGAC	5 cut(s)	88, 475, 820, 1494, 1967
BsnI	GGCC	9 cut(s)	60, 204, 303, 551, 1257, 1316, 1348, 2020, 2107
Bso31I	GGTCTC	1 cut(s)	1582
Bsp119I	TTCGAA	1 cut(s)	1084
Bsp1286I	GDGCHC	8 cut(s)	36, 279, 519, 717, 897, 1036, 1206, 1515
Bsp1407I	TGTACA	2 cut(s)	779, 1011
Bsp143I	GATC	5 cut(s)	126, 348, 612, 1128, 1914
Bsp1720I	GCTNAGC	2 cut(s)	273, 1328
Bsp19I	CCATGG	2 cut(s)	1230, 1467
BspANI	GGCC	9 cut(s)	60, 204, 303, 551, 1257, 1316, 1348, 2020, 2107
BspCNI	CTCAG	5 cut(s)	265, 551, 1341, 1592, 1929
BspHI	TCATGA	1 cut(s)	474
BspMI	ACCTGC	1 cut(s)	1564
BspPI	GGATC	4 cut(s)	134, 343, 356, 620
BspT104I	TTCGAA	1 cut(s)	1084
BspT107I	GGYRCC	5 cut(s)	155, 835, 1154, 1463, 1565
BspTNI	GGTCTC	1 cut(s)	1582
BsrBI	CCGCTC	2 cut(s)	397, 1213
BsrDI	GCAATG	1 cut(s)	1428
BsrFI	RCCGGY	1 cut(s)	377
BsrGI	TGTACA	2 cut(s)	779, 1011
BsrI	ACTGG	3 cut(s)	474, 708, 1650
BssAI	RCCGGY	1 cut(s)	377
BssECI	CCNNGG	9 cut(s)	106, 145, 492, 552, 852, 1230, 1416, 1467, 1676
BssHII	GCGCGC	1 cut(s)	1401
BssMI	GATC	5 cut(s)	126, 348, 612, 1128, 1914
BssNAI	GTATAC	1 cut(s)	1599
BssNI	GRCGYC	1 cut(s)	336
BssSI	CACGAG	1 cut(s)	1382
BssT1I	CCWWGG	3 cut(s)	852, 1230, 1467
Bst1107I	GTATAC	1 cut(s)	1599
Bst2BI	CACGAG	1 cut(s)	1382
Bst2UI	CCWGG	3 cut(s)	346, 671, 1677
Bst6I	CTCTTC	7 cut(s)	53, 1271, 1369, 1621, 1803, 1863, 2059
BstACI	GRCGYC	1 cut(s)	336
BstAUI	TGTACA	2 cut(s)	779, 1011
BstBAI	YACGTR	1 cut(s)	1049
BstBI	TTCGAA	1 cut(s)	1084
BstC8I	GCNNGC	8 cut(s)	262, 1096, 1246, 1350, 1399, 1403, 1585, 1943
BstDEI	CTNAG	6 cut(s)	273, 538, 1328, 1579, 1937, 2035
BstDSI	CCRYGG	4 cut(s)	552, 1230, 1416, 1467
BstF5I	GGATG	4 cut(s)	81, 264, 1116, 1672
BstH2I	RGCGCY	2 cut(s)	81, 860
BstKTI	GATC	5 cut(s)	129, 351, 615, 1131, 1917
BstMAI	GTCTC	4 cut(s)	187, 406, 1152, 1582
BstMBI	GATC	5 cut(s)	126, 348, 612, 1128, 1914
BstMCI	CGRYCG	2 cut(s)	304, 2108
BstNI	CCWGG	3 cut(s)	346, 671, 1677
BstNSI	RCATGY	3 cut(s)	1098, 1381, 1523
BstSCI	CCNGG	5 cut(s)	344, 492, 669, 1635, 1675
BstSFI	CTRYAG	1 cut(s)	2094
BstSNI	TACGTA	1 cut(s)	1049
BstV1I	GCAGC	2 cut(s)	1336, 1344
BstV2I	GAAGAC	2 cut(s)	208, 1727
BstX2I	RGATCY	1 cut(s)	348
BstYI	RGATCY	1 cut(s)	348
BstZ17I	GTATAC	1 cut(s)	1599
BstZI	CGGCCG	2 cut(s)	301, 2105
BsuI	GTATCC	2 cut(s)	573, 715
BsuRI	GGCC	9 cut(s)	60, 204, 303, 551, 1257, 1316, 1348, 2020, 2107
BtgI	CCRYGG	4 cut(s)	552, 1230, 1416, 1467
BtgZI	GCGATG	3 cut(s)	97, 292, 352
BtsCI	GGATG	4 cut(s)	81, 264, 1116, 1672
BtsI	GCAGTG	2 cut(s)	1014, 1353
BtsIMutI	CAGTG	6 cut(s)	177, 546, 715, 1014, 1353, 1643
BveI	ACCTGC	1 cut(s)	1564
Cac8I	GCNNGC	8 cut(s)	262, 1096, 1246, 1350, 1399, 1403, 1585, 1943
CaiI	CAGNNNCTG	2 cut(s)	1355, 1456
CciI	TCATGA	1 cut(s)	474
CciNI	GCGGCCGC	1 cut(s)	2105
Cfr10I	RCCGGY	1 cut(s)	377
Cfr13I	GGNCC	8 cut(s)	203, 412, 489, 632, 667, 1215, 1234, 2018
Cfr42I	CCGCGG	1 cut(s)	555
CseI	GACGC	3 cut(s)	774, 1324, 2088
CsiI	ACCWGGT	1 cut(s)	669
Csp6I	GTAC	5 cut(s)	780, 929, 1012, 1050, 1834
CviQI	GTAC	5 cut(s)	780, 929, 1012, 1050, 1834
DdeI	CTNAG	6 cut(s)	273, 538, 1328, 1579, 1937, 2035
DpnI	GATC	5 cut(s)	128, 350, 614, 1130, 1916
DpnII	GATC	5 cut(s)	126, 348, 612, 1128, 1914
DraI	TTTAAA	1 cut(s)	1371
DriI	GACNNNNNGTC	1 cut(s)	1178
EaeI	YGGCCR	4 cut(s)	301, 549, 1346, 2105
EagI	CGGCCG	2 cut(s)	301, 2105
Eam1104I	CTCTTC	7 cut(s)	53, 1271, 1369, 1621, 1803, 1863, 2059
Eam1105I	GACNNNNNGTC	1 cut(s)	1178
EarI	CTCTTC	7 cut(s)	53, 1271, 1369, 1621, 1803, 1863, 2059
EciI	GGCGGA	2 cut(s)	74, 307
Ecl136II	GAGCTC	4 cut(s)	277, 517, 895, 1204
EclXI	CGGCCG	2 cut(s)	301, 2105
Eco105I	TACGTA	1 cut(s)	1049
Eco130I	CCWWGG	3 cut(s)	852, 1230, 1467
Eco147I	AGGCCT	1 cut(s)	1316
Eco24I	GRGCYC	7 cut(s)	36, 279, 519, 717, 897, 1036, 1206
Eco31I	GGTCTC	1 cut(s)	1582
Eco47I	GGWCC	6 cut(s)	412, 489, 632, 667, 1215, 1234
Eco52I	CGGCCG	2 cut(s)	301, 2105
Eco53kI	GAGCTC	4 cut(s)	277, 517, 895, 1204
Eco57I	CTGAAG	2 cut(s)	183, 2049
EcoICRI	GAGCTC	4 cut(s)	277, 517, 895, 1204
EcoO109I	RGGNCCY	4 cut(s)	203, 412, 489, 667
EcoRII	CCWGG	3 cut(s)	344, 669, 1675
EcoT14I	CCWWGG	3 cut(s)	852, 1230, 1467
EcoT38I	GRGCYC	7 cut(s)	36, 279, 519, 717, 897, 1036, 1206
ErhI	CCWWGG	3 cut(s)	852, 1230, 1467
Esp3I	CGTCTC	2 cut(s)	406, 1152
FalI	AAGNNNNNCTT	2 cut(s)	1934, 1966
FaqI	GGGAC	5 cut(s)	88, 475, 820, 1494, 1967
FauI	CCCGC	6 cut(s)	269, 547, 634, 734, 1118, 1253
FauNDI	CATATG	1 cut(s)	1391
FbaI	TGATCA	1 cut(s)	1128
FblI	GTMKAC	2 cut(s)	1182, 1598
FokI	GGATG	4 cut(s)	68, 251, 1103, 1659
FriOI	GRGCYC	7 cut(s)	36, 279, 519, 717, 897, 1036, 1206
FspBI	CTAG	5 cut(s)	11, 356, 998, 1544, 1559
GsaI	CCCAGC	1 cut(s)	432
HaeII	RGCGCY	2 cut(s)	81, 860
HaeIII	GGCC	9 cut(s)	60, 204, 303, 551, 1257, 1316, 1348, 2020, 2107
HapII	CCGG	4 cut(s)	378, 494, 1636, 2141
HgaI	GACGC	3 cut(s)	774, 1324, 2088
Hin1I	GRCGYC	1 cut(s)	336
HincII	GTYRAC	2 cut(s)	331, 1689
HindII	GTYRAC	2 cut(s)	331, 1689
HindIII	AAGCTT	1 cut(s)	2029
HpaII	CCGG	4 cut(s)	378, 494, 1636, 2141
Hpy166II	GTNNAC	9 cut(s)	331, 975, 1012, 1183, 1237, 1501, 1599, 1689, 1900
Hpy188I	TCNGA	8 cut(s)	163, 1210, 1452, 1517, 1622, 1938, 2027, 2047
Hpy188III	TCNNGA	5 cut(s)	475, 562, 718, 805, 1339
Hpy8I	GTNNAC	9 cut(s)	331, 975, 1012, 1183, 1237, 1501, 1599, 1689, 1900
Hpy99I	CGWCG	7 cut(s)	59, 338, 341, 452, 455, 790, 2104
HpyAV	CCTTC	9 cut(s)	127, 148, 217, 256, 362, 480, 505, 991, 1885
HpyCH4IV	ACGT	2 cut(s)	336, 1048
HpyCH4V	TGCA	4 cut(s)	528, 1192, 1223, 1381
HpyF3I	CTNAG	6 cut(s)	273, 538, 1328, 1579, 1937, 2035
HpySE526I	ACGT	2 cut(s)	336, 1048
Hsp92I	GRCGYC	1 cut(s)	336
Ksp22I	TGATCA	1 cut(s)	1128
KspI	CCGCGG	1 cut(s)	555
Kzo9I	GATC	5 cut(s)	126, 348, 612, 1128, 1914
Lsp1109I	GCAGC	2 cut(s)	1336, 1344
LweI	GCATC	8 cut(s)	120, 159, 273, 315, 537, 813, 1456, 2080
MabI	ACCWGGT	1 cut(s)	669
MaeI	CTAG	5 cut(s)	11, 356, 998, 1544, 1559
MaeII	ACGT	2 cut(s)	336, 1048
MaeIII	GTNAC	3 cut(s)	562, 811, 2000
MalI	GATC	5 cut(s)	128, 350, 614, 1130, 1916
MbiI	CCGCTC	2 cut(s)	397, 1213
MboI	GATC	5 cut(s)	126, 348, 612, 1128, 1914
MflI	RGATCY	1 cut(s)	348
MhlI	GDGCHC	8 cut(s)	36, 279, 519, 717, 897, 1036, 1206, 1515
MluCI	AATT	2 cut(s)	724, 1742
MlyI	GAGTC	6 cut(s)	1151, 1167, 1481, 1880, 1927, 2153
MmeI	TCCRAC	1 cut(s)	1540
MseI	TTAA	3 cut(s)	1370, 1928, 2161
MslI	CAYNNNNRTG	1 cut(s)	311
MspA1I	CMGCKG	2 cut(s)	554, 1760
MspI	CCGG	4 cut(s)	378, 494, 1636, 2141
MspR9I	CCNGG	5 cut(s)	346, 494, 671, 1637, 1677
MvaI	CCWGG	3 cut(s)	346, 671, 1677
NciI	CCSGG	2 cut(s)	494, 1637
NcoI	CCATGG	2 cut(s)	1230, 1467
NdeI	CATATG	1 cut(s)	1391
NdeII	GATC	5 cut(s)	126, 348, 612, 1128, 1914
NmeAIII	GCCGAG	2 cut(s)	87, 126
NmuCI	GTSAC	3 cut(s)	562, 811, 2000
NotI	GCGGCCGC	1 cut(s)	2105
NspI	RCATGY	3 cut(s)	1098, 1381, 1523
NspV	TTCGAA	1 cut(s)	1084
PaeI	GCATGC	1 cut(s)	1098
PagI	TCATGA	1 cut(s)	474
PauI	GCGCGC	1 cut(s)	1401
PceI	AGGCCT	1 cut(s)	1316
PfeI	GAWTC	4 cut(s)	1342, 1540, 1701, 2077
PleI	GAGTC	6 cut(s)	1150, 1167, 1480, 1879, 1927, 2152
PpsI	GAGTC	6 cut(s)	1150, 1167, 1480, 1879, 1927, 2152
Ppu21I	YACGTR	1 cut(s)	1049
PpuMI	RGGWCCY	3 cut(s)	412, 489, 667
Psp124BI	GAGCTC	4 cut(s)	279, 519, 897, 1206
Psp5II	RGGWCCY	3 cut(s)	412, 489, 667
Psp6I	CCWGG	3 cut(s)	344, 669, 1675
PspFI	CCCAGC	1 cut(s)	428
PspGI	CCWGG	3 cut(s)	344, 669, 1675
PspPI	GGNCC	8 cut(s)	203, 412, 489, 632, 667, 1215, 1234, 2018
PspPPI	RGGWCCY	3 cut(s)	412, 489, 667
PstNI	CAGNNNCTG	2 cut(s)	1355, 1456
PsuI	RGATCY	1 cut(s)	348
PteI	GCGCGC	1 cut(s)	1401
PvuII	CAGCTG	1 cut(s)	1760
RsaI	GTAC	5 cut(s)	781, 930, 1013, 1051, 1835
RsaNI	GTAC	5 cut(s)	780, 929, 1012, 1050, 1834
RseI	CAYNNNNRTG	1 cut(s)	311
SacI	GAGCTC	4 cut(s)	279, 519, 897, 1206
SacII	CCGCGG	1 cut(s)	555
SaqAI	TTAA	3 cut(s)	1370, 1928, 2161
Sau3AI	GATC	5 cut(s)	126, 348, 612, 1128, 1914
Sau96I	GGNCC	8 cut(s)	203, 412, 489, 632, 667, 1215, 1234, 2018
SchI	GAGTC	6 cut(s)	1151, 1167, 1481, 1880, 1927, 2153
ScrFI	CCNGG	5 cut(s)	346, 494, 671, 1637, 1677
SduI	GDGCHC	8 cut(s)	36, 279, 519, 717, 897, 1036, 1206, 1515
SexAI	ACCWGGT	1 cut(s)	669
SfaNI	GCATC	8 cut(s)	120, 159, 273, 315, 537, 813, 1456, 2080
SfcI	CTRYAG	1 cut(s)	2094
Sfr303I	CCGCGG	1 cut(s)	555
SfuI	TTCGAA	1 cut(s)	1084
SgrBI	CCGCGG	1 cut(s)	555
SinI	GGWCC	6 cut(s)	412, 489, 632, 667, 1215, 1234
SmiMI	CAYNNNNRTG	1 cut(s)	311
SmlI	CTYRAG	3 cut(s)	62, 278, 716
SmoI	CTYRAG	3 cut(s)	62, 278, 716
SnaBI	TACGTA	1 cut(s)	1049
SpeI	ACTAGT	1 cut(s)	997
SphI	GCATGC	1 cut(s)	1098
Sse9I	AATT	2 cut(s)	724, 1742
SseBI	AGGCCT	1 cut(s)	1316
SspI	AATATT	2 cut(s)	910, 1089
SspMI	CTAG	5 cut(s)	11, 356, 998, 1544, 1559
SstI	GAGCTC	4 cut(s)	279, 519, 897, 1206
StuI	AGGCCT	1 cut(s)	1316
StyD4I	CCNGG	5 cut(s)	344, 492, 669, 1635, 1675
StyI	CCWWGG	3 cut(s)	852, 1230, 1467
TaiI	ACGT	2 cut(s)	339, 1051
TaqI	TCGA	3 cut(s)	821, 1084, 1140
TasI	AATT	2 cut(s)	724, 1742
TatI	WGTACW	3 cut(s)	779, 1011, 1833
TfiI	GAWTC	4 cut(s)	1342, 1540, 1701, 2077
Tru1I	TTAA	3 cut(s)	1370, 1928, 2161
Tru9I	TTAA	3 cut(s)	1370, 1928, 2161
TscAI	CASTG	6 cut(s)	184, 546, 715, 1014, 1360, 1650
TseFI	GTSAC	3 cut(s)	562, 811, 2000
TseI	GCWGC	2 cut(s)	1324, 1332
Tsp45I	GTSAC	3 cut(s)	562, 811, 2000
TspDTI	ATGAA	3 cut(s)	887, 973, 1821
TspGWI	ACGGA	2 cut(s)	394, 624
TspRI	CASTG	6 cut(s)	184, 546, 715, 1014, 1360, 1650
VpaK11BI	GGWCC	6 cut(s)	412, 489, 632, 667, 1215, 1234
XceI	RCATGY	3 cut(s)	1098, 1381, 1523
XcmI	CCANNNNNNNNNTGG	2 cut(s)	1195, 1474
XmiI	GTMKAC	2 cut(s)	1182, 1598
XspI	CTAG	5 cut(s)	11, 356, 998, 1544, 1559
ZraI	GACGTC	1 cut(s)	337

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.