Rh4AG279900

Cation H( ) antiporter

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr4A
OG0004570
Physical Location & Seq
Forward (+)
59557364 .. 59558262
899 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh4AG279900.1

Sequence Viewer

Length: 645 bp
ATGAAGGCCACTTCGAATGGATCATTTCAGGGCGAAAATCCACTTGATTACACTCTTCCCTTACTCATCGTCCAGATCATCTTGGTCGTGGTGTTCACCAGGTTTCTTGCATATCTTCTAAAACCTCTCCGGCAACCCCGAGTCATAGCCGAAGTTATAGGCGGATGTCTGCTAGGGCCCTCCGCATTCAGGAGGAGCAAGACCTTTCTACACACGATTTTCCCCCCAAAAGGCATGACCATGCTAGATACAGTAGCCAACATCAGGCTCTATTTCTTCTTCTTCCTCGTCGGCCTCGAGCTCGACATTGACGCCATCCGCAGCACCGGAAAGAAGTCCCTCGGCATCGCCCTCTGCGAGATCACCCTCCCCTTCGTCCTCGGCATCGGCACCTCCGAAGCCCTACGCACCACCGTCTCGAAAGGCTTGAAACAAGGCCCCTTCCTCGTCTTCAACGGCGTCGCCCTCTCCATCACTGCCTTCCCCGTCCTGGCCCGCATCCTCGTTGAGCTCAAGCTCCTCACCACCGATGTCGGCTGCATTGCCATGTCCGTCGCCGGCGTCAACGACGCCGCCGCCTGGATCCTTCTCGCCCTCACCGGCAACAACACCTCACCGCTTGTCTCTGTCTGGGTCCTCCTCTGA

Protein Analysis

214

Amino Acids

23.12

Weight (kDa)

9.47

Isoelectric Point (pI)

30.93

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Na_H_Exchanger PF00999 32 - 200 1.9e-21 Sodium/hydrogen exchanger, transmembrane
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 389
AciI CCGC 7 cut(s) 162, 183, 319, 496, 573, 576, 617
AclWI GGATC 3 cut(s) 28, 577, 590
AcyI GRCGYC 4 cut(s) 312, 459, 561, 570
AfiI CCNNNNNNNGG 5 cut(s) 189, 230, 264, 490, 579
AgsI TTSAA 2 cut(s) 430, 454
AjnI CCWGG 3 cut(s) 98, 489, 578
AluBI AGCT 3 cut(s) 301, 511, 517
AluI AGCT 3 cut(s) 301, 511, 517
Alw21I GWGCWC 2 cut(s) 303, 513
Alw26I GTCTC 2 cut(s) 421, 628
AlwI GGATC 3 cut(s) 28, 577, 590
Ama87I CYCGRG 2 cut(s) 138, 296
AoxI GGCC 5 cut(s) 6, 176, 292, 436, 492
ApaI GGGCCC 1 cut(s) 180
ApeKI GCWGC 2 cut(s) 321, 537
AspS9I GGNCC 5 cut(s) 176, 177, 437, 493, 634
AsuHPI GGTGA 5 cut(s) 88, 355, 514, 589, 606
AsuII TTCGAA 1 cut(s) 14
AvaI CYCGRG 2 cut(s) 138, 296
AvaII GGWCC 1 cut(s) 634
BaeGI GKGCMC 1 cut(s) 180
BamHI GGATCC 1 cut(s) 582
BanI GGYRCC 1 cut(s) 389
BanII GRGCYC 3 cut(s) 180, 303, 513
BbsI GAAGAC 1 cut(s) 442
Bbv12I GWGCWC 2 cut(s) 303, 513
BbvI GCAGC 2 cut(s) 333, 524
BccI CCATC 2 cut(s) 323, 479
BceAI ACGGC 1 cut(s) 472
BciT130I CCWGG 3 cut(s) 100, 491, 580
BcoDI GTCTC 2 cut(s) 421, 628
BfaI CTAG 2 cut(s) 173, 245
BisI GCNGC 4 cut(s) 322, 538, 573, 576
BlsI GCNGC 4 cut(s) 323, 539, 574, 577
Bme1390I CCNGG 3 cut(s) 100, 491, 580
Bme18I GGWCC 1 cut(s) 634
BmeT110I CYCGRG 2 cut(s) 138, 296
BmgT120I GGNCC 5 cut(s) 176, 177, 437, 493, 634
BmiI GGNNCC 5 cut(s) 178, 391, 439, 584, 635
BmrFI CCNGG 3 cut(s) 100, 491, 580
BmsI GCATC 3 cut(s) 354, 393, 507
BpiI GAAGAC 1 cut(s) 442
Bpu14I TTCGAA 1 cut(s) 14
BpuEI CTTGAG 1 cut(s) 497
BsaHI GRCGYC 4 cut(s) 312, 459, 561, 570
BsaJI CCNNGG 2 cut(s) 340, 379
BsaWI WCCGGW 1 cut(s) 326
Bsc4I CCNNNNNNNGG 5 cut(s) 189, 230, 264, 490, 579
Bse118I RCCGGY 2 cut(s) 557, 599
Bse3DI GCAATG 1 cut(s) 540
BseBI CCWGG 3 cut(s) 100, 491, 580
BseDI CCNNGG 2 cut(s) 340, 379
BseGI GGATG 3 cut(s) 170, 315, 498
BseLI CCNNNNNNNGG 5 cut(s) 189, 230, 264, 490, 579
BseMI GCAATG 1 cut(s) 540
BseRI GAGGAG 3 cut(s) 208, 509, 629
BseSI GKGCMC 1 cut(s) 180
BseXI GCAGC 2 cut(s) 333, 524
BshFI GGCC 5 cut(s) 8, 178, 294, 438, 494
BshNI GGYRCC 1 cut(s) 389
BsiHKAI GWGCWC 2 cut(s) 303, 513
BsiHKCI CYCGRG 2 cut(s) 138, 296
BsiSI CCGG 4 cut(s) 130, 327, 558, 600
BslFI GGGAC 1 cut(s) 322
BslI CCNNNNNNNGG 5 cut(s) 189, 230, 264, 490, 579
BsmAI GTCTC 2 cut(s) 421, 628
BsmBI CGTCTC 1 cut(s) 421
BsmFI GGGAC 1 cut(s) 322
BsmI GAATGC 1 cut(s) 185
BsnI GGCC 5 cut(s) 8, 178, 294, 438, 494
BsoBI CYCGRG 2 cut(s) 138, 296
Bsp119I TTCGAA 1 cut(s) 14
Bsp120I GGGCCC 1 cut(s) 176
Bsp1286I GDGCHC 3 cut(s) 180, 303, 513
Bsp143I GATC 4 cut(s) 20, 75, 360, 582
BspACI CCGC 7 cut(s) 162, 183, 319, 496, 573, 576, 617
BspANI GGCC 5 cut(s) 8, 178, 294, 438, 494
BspLI GGNNCC 5 cut(s) 178, 391, 439, 584, 635
BspPI GGATC 3 cut(s) 28, 577, 590
BspT104I TTCGAA 1 cut(s) 14
BspT107I GGYRCC 1 cut(s) 389
BsrDI GCAATG 1 cut(s) 540
BsrFI RCCGGY 2 cut(s) 557, 599
BssAI RCCGGY 2 cut(s) 557, 599
BssECI CCNNGG 2 cut(s) 340, 379
BssMI GATC 4 cut(s) 20, 75, 360, 582
BssNI GRCGYC 4 cut(s) 312, 459, 561, 570
Bst2UI CCWGG 3 cut(s) 100, 491, 580
Bst4CI ACNGT 2 cut(s) 253, 415
Bst6I CTCTTC 1 cut(s) 60
BstACI GRCGYC 4 cut(s) 312, 459, 561, 570
BstBI TTCGAA 1 cut(s) 14
BstC8I GCNNGC 2 cut(s) 496, 559
BstF5I GGATG 3 cut(s) 170, 315, 498
BstKTI GATC 4 cut(s) 23, 78, 363, 585
BstMAI GTCTC 2 cut(s) 421, 628
BstMBI GATC 4 cut(s) 20, 75, 360, 582
BstNI CCWGG 3 cut(s) 100, 491, 580
BstSCI CCNGG 3 cut(s) 98, 489, 578
BstSLI GKGCMC 1 cut(s) 180
BstV1I GCAGC 2 cut(s) 333, 524
BstV2I GAAGAC 1 cut(s) 442
BstX2I RGATCY 1 cut(s) 582
BstYI RGATCY 1 cut(s) 582
BsuRI GGCC 5 cut(s) 8, 178, 294, 438, 494
BtgZI GCGATG 1 cut(s) 331
BtsCI GGATG 3 cut(s) 170, 315, 498
BtsI GCAGTG 1 cut(s) 474
BtsIMutI CAGTG 1 cut(s) 474
Cac8I GCNNGC 2 cut(s) 496, 559
Cfr10I RCCGGY 2 cut(s) 557, 599
Cfr13I GGNCC 5 cut(s) 176, 177, 437, 493, 634
CseI GACGC 4 cut(s) 320, 448, 550, 578
CsiI ACCWGGT 1 cut(s) 98
CviAII CATG 3 cut(s) 235, 241, 547
DpnI GATC 4 cut(s) 22, 77, 362, 584
DpnII GATC 4 cut(s) 20, 75, 360, 582
Eam1104I CTCTTC 1 cut(s) 60
EarI CTCTTC 1 cut(s) 60
EciI GGCGGA 1 cut(s) 177
Ecl136II GAGCTC 2 cut(s) 301, 511
Eco24I GRGCYC 3 cut(s) 180, 303, 513
Eco47I GGWCC 1 cut(s) 634
Eco53kI GAGCTC 2 cut(s) 301, 511
Eco88I CYCGRG 2 cut(s) 138, 296
EcoICRI GAGCTC 2 cut(s) 301, 511
EcoO109I RGGNCCY 4 cut(s) 176, 177, 437, 634
EcoRII CCWGG 3 cut(s) 98, 489, 578
EcoT38I GRGCYC 3 cut(s) 180, 303, 513
Esp3I CGTCTC 1 cut(s) 421
FaeI CATG 3 cut(s) 238, 244, 550
FaiI YATR 6 cut(s) 112, 146, 158, 236, 242, 548
FaqI GGGAC 1 cut(s) 322
FatI CATG 3 cut(s) 234, 240, 546
FauI CCCGC 1 cut(s) 503
Fnu4HI GCNGC 4 cut(s) 322, 538, 573, 576
FokI GGATG 3 cut(s) 177, 302, 485
FriOI GRGCYC 3 cut(s) 180, 303, 513
Fsp4HI GCNGC 4 cut(s) 322, 538, 573, 576
FspBI CTAG 2 cut(s) 173, 245
GluI GCNGC 4 cut(s) 322, 538, 573, 576
HaeIII GGCC 5 cut(s) 8, 178, 294, 438, 494
HapII CCGG 4 cut(s) 130, 327, 558, 600
HgaI GACGC 4 cut(s) 320, 448, 550, 578
Hin1I GRCGYC 4 cut(s) 312, 459, 561, 570
Hin1II CATG 3 cut(s) 238, 244, 550
HincII GTYRAC 1 cut(s) 565
HindII GTYRAC 1 cut(s) 565
HinfI GANTC 1 cut(s) 141
HpaII CCGG 4 cut(s) 130, 327, 558, 600
HphI GGTGA 5 cut(s) 88, 355, 514, 589, 606
Hpy166II GTNNAC 2 cut(s) 96, 565
Hpy188I TCNGA 2 cut(s) 397, 644
Hpy188III TCNNGA 3 cut(s) 73, 190, 418
Hpy8I GTNNAC 2 cut(s) 96, 565
Hpy99I CGWCG 4 cut(s) 293, 464, 557, 572
HpyAV CCTTC 4 cut(s) 382, 451, 490, 596
HpyCH4III ACNGT 2 cut(s) 253, 415
HpyCH4V TGCA 2 cut(s) 110, 540
Hsp92I GRCGYC 4 cut(s) 312, 459, 561, 570
Hsp92II CATG 3 cut(s) 238, 244, 550
KroI GCCGGC 1 cut(s) 557
KroNI GCCGGC 1 cut(s) 559
Kzo9I GATC 4 cut(s) 20, 75, 360, 582
LmnI GCTCC 2 cut(s) 195, 522
Lsp1109I GCAGC 2 cut(s) 333, 524
LweI GCATC 3 cut(s) 354, 393, 507
MabI ACCWGGT 1 cut(s) 98
MaeI CTAG 2 cut(s) 173, 245
MalI GATC 4 cut(s) 22, 77, 362, 584
MboI GATC 4 cut(s) 20, 75, 360, 582
MboII GAAGA 6 cut(s) 47, 107, 268, 271, 274, 442
MflI RGATCY 1 cut(s) 582
MhlI GDGCHC 3 cut(s) 180, 303, 513
MlyI GAGTC 1 cut(s) 150
MreI CGCCGGCG 1 cut(s) 557
MroNI GCCGGC 1 cut(s) 557
MslI CAYNNNNRTG 2 cut(s) 239, 545
MspI CCGG 4 cut(s) 130, 327, 558, 600
MspR9I CCNGG 3 cut(s) 100, 491, 580
Mva1269I GAATGC 1 cut(s) 185
MvaI CCWGG 3 cut(s) 100, 491, 580
NaeI GCCGGC 1 cut(s) 559
NdeII GATC 4 cut(s) 20, 75, 360, 582
NgoMIV GCCGGC 1 cut(s) 557
NlaIII CATG 3 cut(s) 238, 244, 550
NlaIV GGNNCC 5 cut(s) 178, 391, 439, 584, 635
NmeAIII GCCGAG 2 cut(s) 321, 360
NspV TTCGAA 1 cut(s) 14
PaeR7I CTCGAG 1 cut(s) 296
PcsI WCGNNNNNNNCGW 3 cut(s) 294, 354, 393
PctI GAATGC 1 cut(s) 185
PdiI GCCGGC 1 cut(s) 559
PkrI GCNGC 4 cut(s) 323, 539, 574, 577
PleI GAGTC 1 cut(s) 149
PpsI GAGTC 1 cut(s) 149
PpuMI RGGWCCY 1 cut(s) 634
Psp124BI GAGCTC 2 cut(s) 303, 513
Psp5II RGGWCCY 1 cut(s) 634
Psp6I CCWGG 3 cut(s) 98, 489, 578
PspGI CCWGG 3 cut(s) 98, 489, 578
PspN4I GGNNCC 5 cut(s) 178, 391, 439, 584, 635
PspOMI GGGCCC 1 cut(s) 176
PspPI GGNCC 5 cut(s) 176, 177, 437, 493, 634
PspPPI RGGWCCY 1 cut(s) 634
PspXI VCTCGAGB 1 cut(s) 296
PsuI RGATCY 1 cut(s) 582
RseI CAYNNNNRTG 2 cut(s) 239, 545
SacI GAGCTC 2 cut(s) 303, 513
SatI GCNGC 4 cut(s) 322, 538, 573, 576
Sau3AI GATC 4 cut(s) 20, 75, 360, 582
Sau96I GGNCC 5 cut(s) 176, 177, 437, 493, 634
SchI GAGTC 1 cut(s) 150
ScrFI CCNGG 3 cut(s) 100, 491, 580
SduI GDGCHC 3 cut(s) 180, 303, 513
SetI ASST 8 cut(s) 104, 127, 206, 303, 395, 513, 519, 614
SexAI ACCWGGT 1 cut(s) 98
SfaNI GCATC 3 cut(s) 354, 393, 507
Sfr274I CTCGAG 1 cut(s) 296
SfuI TTCGAA 1 cut(s) 14
SgrAI CRCCGGYG 1 cut(s) 557
SinI GGWCC 1 cut(s) 634
SlaI CTCGAG 1 cut(s) 296
SmiMI CAYNNNNRTG 2 cut(s) 239, 545
SmlI CTYRAG 2 cut(s) 296, 512
SmoI CTYRAG 2 cut(s) 296, 512
SsiI CCGC 7 cut(s) 162, 183, 319, 496, 573, 576, 617
SspMI CTAG 2 cut(s) 173, 245
SstI GAGCTC 2 cut(s) 303, 513
StyD4I CCNGG 3 cut(s) 98, 489, 578
TaaI ACNGT 2 cut(s) 253, 415
TaqI TCGA 4 cut(s) 14, 297, 303, 419
TauI GCSGC 2 cut(s) 575, 578
TscAI CASTG 1 cut(s) 481
TseI GCWGC 2 cut(s) 321, 537
TspDTI ATGAA 1 cut(s) 17
TspGWI ACGGA 1 cut(s) 541
TspRI CASTG 1 cut(s) 481
VpaK11BI GGWCC 1 cut(s) 634
XhoI CTCGAG 1 cut(s) 296
XspI CTAG 2 cut(s) 173, 245
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.