Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr13

Orthogroup ID

OG0001572

Physical Location & Seq

Genomic Coordinates Forward (+)

10942469 .. 10948637

Gene Structure 6169 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

MD13G1141300.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 3066 bp

ATGGAGGCTTTTGGAGGCAGAGCTCGGAATTTCTATGGTACGATGGTGCCGGATTTGAAGGGGGCTGGGAAAAAGAGTTTGGAATGGGATTTGAATGATTGGAAATGGGATGGTGATCTTTTTACCGCTAGTCCTTTGAATGCTGTACCATCTGATTGTAGGAGCAGGCAGTTGTTTCCGCTTGGGCTGCCGGAAACTCCCTCCACTGCTGGTTTGTCCAACAGTTCTTCTTCTGGTTCGGACGGTATCTGTCCAGGGAATGAGAAAGGTAAAAGAGAGCTGGAGAAAAGAAGAAGGGCTAGTTTTGTAGAAAATGAAGGGCTAACTGATGAAGTTGGGTCTCTTAATCTCAAGCTTGGCGGGCAAGCTTACCCAATCATGGAAGGAGAGGTACAACATGGGAAGAAGACGAAGATAGTTGGGACTACTTTAAACCGAGCAGTTTGTCAGGTGGAGGACTGTAAGGCTGATCTTAGCAATGCCAAGGATTACCACCGACGACATAAGGTCTGTGATATGCATTCTAAGGCAACTAAAGCGGTGGTGGGAAATGTTTTGCAGCGGTTCTGTCAGCAGTGTAGCAGGTTTCATGGCCTTCAAGAGTTTGATGAAGGAAAGAGAAGTTGTCGTAGACGTTTGGCAGGCCACAATAGGAGGAGAAGAAAAACACATCCTGATACTGTAGTTAATGGAGGCTCATTGAATGATGAAAGAGGAAGCAGTTATCTATTGATTAGTTTGCTGAGAATACTCTCCAACATGCACTCTAATAGTTCAGATCAAACAAAGGATCAGGATTTGCTATCTCATCTTTTGAAGAATCTAGCCAATCTTTCTGGTACAGTTGATGGAAGAAACATGTCTGCATTGCTTACGGCATCTCAAGGTTTAATAAATGGCGGGGCATCAATTCAGACTGCACAAAAGGTCCCAGATACAGTTTCCAACGGCTGTGAACCAAGTAAGCCTTCTGTTTCAGCCTCTAAAATGGATGATTATATTAATGGTGAGGACCCTTCAAGACCTATACGACAGTGTTCTACGGTACCTGCATCAGATTTCAGGAGAATATCTTCAGTTGATGCAGATCATGGAGGTCTACAAGTTGTTTCAGGTCTAAATGCCACGAAGCCATTTCCTTCAAGAGACAGTGTTCCATCCACATCAGTTGCACCAGAAGCTACAATGGGGAGGATGCAGTTAAATGGCATTGATTTAAATAATACATATGACGATTCCCAGGACTATTTGGACAACCTAGGGAATTCGCATGCCCCTGTAAATTCAGGGACAGTGGCTCATGGTTTTCCCTTTTGGATGCGTCAGGATTCGCAAAAGTCTAGCCCACCTCAGACAAGTGGGACTTCATGCTCAACTTCATCAAGTTCTAGTGGAGATGCTCAGAGTCGCACAGACCGTATTGTTTTTAAACTCTTTGGAAAAGATCCAAATGATCTTCCATTTGTTCTACGAGCACAGATTCTCAACTGGTTGTCCCACAGTCCTACTGACATTGAAAGCTACATAAGGCCGGGATGTATCATTTTGACAGTTTACCTCCGTCTAGAAAAATCCACGTGGGAGGAGCTCTGCTGTAATCTGGGATCCATTATAAAACAACTTTTACATGCTGCCAATGATCCTTTTTGGACAACAGGATGGGTGTATACTAGGGTGCAACATTCTGTAGCATTTACGTACAATGGTCAAGTTGTCTTAGACACACCCTTACCTCTGAAAAGCCATAAAAATTGCAAGATATCATGCATCAAACCAATTGCAGTTTCTTTATCCGAAAGAGCTGAATTTGTAGTAAAGGGGTTTAATCTTTCTCGTGCCACCACAAGGTTGCTCTGTGCTCTGGAAGGGAAGTATCTAGTCCAAGAAACTTGTTATGACTTGATGGATGGTGCTGATACAACCTTTGCGAATGATCAGCTACAGTGCCTCAGATTCTCCTGCTCTATACCCAATGTTACTGGCCGAGGACTCATTGAGGTTGAAGACCATGGTCTCAGCGGCTGCTTCTTTCCATTTATAGTTGCCGAGCAGGAAGTATGTTCAGAGATTTGTACCCTGGAGGGTGCGATTGAGGTGGCTGAGACTGCAGATAACATCCAGACAGAACCTGAAAAGTTGGAAGCCAAGAACCAAGCGTTGGACTTTGTACATGAATTGGGTTGGCTCCTTCACAGATGTCACACTAAATTTAGACTTGGTCACGGGGATCCCAACTTAGAACTATTTTCTTTTAGACGGTTCAGGTTGCTTATGGAGTTTTCCATGGACCGTGATTGGTGTGCTGTAGTGAAGAAACTCCTGGGCATTCTGCTTGAGGGTACCGTTGACACTGGAGAACATCCTTCTATTGAGCTTGCGTTATTGGATATGAGCCTCCTCCACGGAGCCGTGCAAAGAAAGTGCAGGCCTATGGTGGAACTCTTGTTAAGATTTGTCCTAGACAAAGGGTGGCAACAAGTTGACGGGGACGGCAGCAACTTCTTATTTAAACCCGATGCAGTTGGGCCCATGGGATTGACTCCTCTTCATGTTGCAGCCAGTACTGATGGATGTGAGAATATATTGGATGCCTTAACTGATGATCCTGGAAAGGTGGGAATTGAAGCGTGGAAAAATGCTCGAGACAGTACAGGATTGACACCAAATGATTATGCATGCCTGCGAGGCCGCTACACCTATGTCCAAATTGTCCAGCGGAAAATCAACAAGAAACATGAAAGCGGGCACGTGGTGCTTGATATCCCTGGCGTTATCTTAGACAGCAGCAGCAAGCAGAAACAATTAGATGGGCATAAGTCCTCAAAAGTCTCCATCTTGGAGACCGAAAGGATCGATATGAAAGCAATGCAGGCACATTGCAAGCAATGTGAAATGAAACTAGCATATGGAAACACAAGGTCGTTTGTGTACAGGCCGGCAATGCTGTCAATGGTAGCCATTGCTGCTGTCTGTGTTTGCGTGGCCTTGCTCTTCAAAAGCTCGCCTGAAGTCCTTTATGTCTTCCAGCCCTTCAGGTGGGAACTCTTGGAGTATGGACCGAGCTAA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003700 GO:0006355 GO:0008150 GO:0009889 GO:0010468 GO:0010556 GO:0019219 GO:0019222 GO:0031323 GO:0031326 GO:0050789 GO:0050794 GO:0051171 GO:0051252 GO:0060255 GO:0065007 GO:0080090 GO:0140110 GO:1903506 GO:2000112 GO:2001141

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

SBP

Protein Analysis

1022

Amino Acids

112.72

Weight (kDa)

6.74

Isoelectric Point (pI)

42.42

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
SBP	PF03110	149 - 222	4e-30	SBP domain
Ig_SPL7	PF26102	582 - 692	1e-42	SPL7-like, immunoglobulin-like domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0001572)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT2G47070 AT2G47070 AT3G60030
fragaria_vesca	FvH4_4g21420 FvH4_4g21420 FvH4_4g21420 FvH4_4g21420 FvH4_4g21420 FvH4_4g21420 FvH4_4g21420 FvH4_4g21420 FvH4_4g21420 FvH4_4g21420 FvH4_4g21420 FvH4_4g21420 FvH4_4g21420 FvH4_4g21420 FvH4_4g21420 FvH4_4g21420
malus_domestica	MD13G1141300.v1.1 MD13G1141400.v1.1 MD16G1137600.v1.1 MD16G1137700.v1.1
prunus_persica	Prupe.1G106500_v2.0.a1 Prupe.1G106500_v2.0.a1 Prupe.1G106600_v2.0.a1
pyrus_communis	pycom13g12280 pycom13g12290 pycom16g12140 pycom16g12150 pycom16g12160
rosa_chinensis	RchiOBHm_Chr4g0427341 RchiOBHm_Chr4g0427351
rosa_laevigata	RLG00000007250 RLG00000007251
rosa_multiflora	Rmu_sc0003813.1_g000017 Rmu_sc0003813.1_g000018
rosa_roxburghii	Rroxscaffold_5G00369020 Rroxscaffold_5G00369030
rosa_rugosa	Rorug04G0216800
rosa_samantha	Rh4AG273100 Rh4AG273300 Rh4BG278800 Rh4BG278900 Rh4CG294100 Rh4CG294200 Rh4DG276200 Rh4DG276300
rosa_wichuraiana	Rw4G023700 Rw4G023710

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	1 cut(s)	1613
Acc36I	ACCTGC	2 cut(s)	573, 1057
Acc65I	GGTACC	2 cut(s)	1045, 2339
AccB1I	GGYRCC	3 cut(s)	46, 1045, 2339
AccB7I	CCANNNNNTGG	1 cut(s)	2158
AccI	GTMKAC	3 cut(s)	631, 1099, 1667
AclWI	GGATC	9 cut(s)	798, 1439, 1599, 1612, 1634, 2222, 2235, 2597, 2858
AcoI	YGGCCR	1 cut(s)	1981
AcsI	RAATTY	5 cut(s)	28, 1264, 1282, 1805, 2207
AcuI	CTGAAG	3 cut(s)	1059, 3016, 3027
AcvI	CACGTG	2 cut(s)	1578, 2749
AdeI	CACNNNGTG	1 cut(s)	2752
AfiI	CCNNNNNNNGG	4 cut(s)	379, 1845, 2158, 3036
AflIII	ACRYGT	1 cut(s)	858
AhdI	GACNNNNNGTC	1 cut(s)	506
AjnI	CCWGG	6 cut(s)	253, 1239, 2076, 2319, 2605, 2764
AjuI	GAANNNNNNNTTGG	8 cut(s)	62, 94, 952, 984, 1768, 1800, 2141, 2173
Alw21I	GWGCWC	4 cut(s)	25, 1477, 1590, 1861
Alw26I	GTCTC	7 cut(s)	345, 1140, 2018, 2096, 2637, 2833, 2834
AlwI	GGATC	9 cut(s)	798, 1439, 1599, 1612, 1634, 2222, 2235, 2597, 2858
AlwNI	CAGNNNCTG	2 cut(s)	2022, 2129
Ama87I	CYCGRG	1 cut(s)	2640
AoxI	GGCC	9 cut(s)	592, 643, 1529, 1981, 2426, 2525, 2686, 2933, 2982
ApaI	GGGCCC	1 cut(s)	2529
ApeKI	GCWGC	9 cut(s)	187, 559, 1631, 2022, 2493, 2555, 2784, 2787, 2963
ApoI	RAATTY	5 cut(s)	28, 1264, 1282, 1805, 2207
AseI	ATTAAT	1 cut(s)	1002
Asp700I	GAANNNNTTC	1 cut(s)	1072
Asp718I	GGTACC	2 cut(s)	1045, 2339
AspA2I	CCTAGG	1 cut(s)	1258
AspS9I	GGNCC	6 cut(s)	928, 1012, 2287, 2525, 2526, 3056
AsuC2I	CCSGG	1 cut(s)	1533
AsuHPI	GGTGA	2 cut(s)	125, 1019
AvaI	CYCGRG	1 cut(s)	2640
AvaII	GGWCC	4 cut(s)	928, 1012, 2287, 3056
AvrII	CCTAGG	1 cut(s)	1258
BaeGI	GKGCMC	2 cut(s)	2529, 2748
BaeI	ACNNNNGTAYC	4 cut(s)	30, 63, 669, 702
BamHI	GGATCC	2 cut(s)	1604, 2227
BanI	GGYRCC	3 cut(s)	46, 1045, 2339
BanII	GRGCYC	3 cut(s)	25, 1590, 2529
BarI	GAAGNNNNNNTAC	2 cut(s)	375, 407
BauI	CACGAG	1 cut(s)	1833
BbrPI	CACGTG	2 cut(s)	1578, 2749
BbsI	GAAGAC	3 cut(s)	413, 2010, 3013
Bbv12I	GWGCWC	4 cut(s)	25, 1477, 1590, 1861
BbvI	GCAGC	9 cut(s)	174, 571, 1618, 2009, 2505, 2567, 2796, 2799, 2950
BceAI	ACGGC	4 cut(s)	891, 964, 2393, 2506
BciT130I	CCWGG	6 cut(s)	255, 1241, 2078, 2321, 2607, 2766
BclI	TGATCA	1 cut(s)	1933
BcnI	CCSGG	1 cut(s)	1533
BcoDI	GTCTC	7 cut(s)	345, 1140, 2018, 2096, 2637, 2833, 2834
BfmI	CTRYAG	5 cut(s)	681, 1686, 1940, 2106, 2304
BfuAI	ACCTGC	2 cut(s)	573, 1057
BglI	GCCNNNNNGGC	1 cut(s)	2685
BlnI	CCTAGG	1 cut(s)	1258
BmcAI	AGTACT	1 cut(s)	2563
Bme1390I	CCNGG	7 cut(s)	255, 1241, 1533, 2078, 2321, 2607, 2766
Bme18I	GGWCC	4 cut(s)	928, 1012, 2287, 3056
BmeRI	GACNNNNNGTC	1 cut(s)	506
BmeT110I	CYCGRG	1 cut(s)	2640
BmgT120I	GGNCC	6 cut(s)	928, 1012, 2287, 2525, 2526, 3056
BmrFI	CCNGG	7 cut(s)	255, 1241, 1533, 2078, 2321, 2607, 2766
BoxI	GACNNNNGTC	1 cut(s)	2010
BpiI	GAAGAC	3 cut(s)	413, 2010, 3013
BpmI	CTGGAG	3 cut(s)	302, 2099, 2373
BpuEI	CTTGAG	3 cut(s)	335, 867, 2354
BpuMI	CCSGG	1 cut(s)	1533
Bsa29I	ATCGAT	1 cut(s)	2853
BsaAI	YACGTR	3 cut(s)	1578, 1698, 2749
BsaBI	GATNNNNATC	2 cut(s)	114, 1086
BsaI	GGTCTC	3 cut(s)	345, 2018, 2834
Bsc4I	CCNNNNNNNGG	4 cut(s)	379, 1845, 2158, 3036
Bse118I	RCCGGY	1 cut(s)	2935
Bse1I	ACTGG	4 cut(s)	1493, 1984, 2356, 2559
Bse3DI	GCAATG	7 cut(s)	484, 866, 2871, 2875, 2891, 2946, 2958
Bse8I	GATNNNNATC	2 cut(s)	114, 1086
BseBI	CCWGG	6 cut(s)	255, 1241, 2078, 2321, 2607, 2766
BseCI	ATCGAT	1 cut(s)	2853
BseJI	GATNNNNATC	2 cut(s)	114, 1086
BseLI	CCNNNNNNNGG	4 cut(s)	379, 1845, 2158, 3036
BseMI	GCAATG	7 cut(s)	484, 866, 2871, 2875, 2891, 2946, 2958
BseMII	CTCAG	6 cut(s)	734, 1364, 1415, 1963, 2029, 2091
BseNI	ACTGG	4 cut(s)	1493, 1984, 2356, 2559
BseRI	GAGGAG	4 cut(s)	670, 1598, 2387, 2532
BseSI	GKGCMC	2 cut(s)	2529, 2748
BseXI	GCAGC	9 cut(s)	174, 571, 1618, 2009, 2505, 2567, 2796, 2799, 2950
BseYI	CCCAGC	1 cut(s)	65
BsgI	GTGCAG	2 cut(s)	903, 2443
BshFI	GGCC	9 cut(s)	594, 645, 1531, 1983, 2428, 2527, 2688, 2935, 2984
BshNI	GGYRCC	3 cut(s)	46, 1045, 2339
BshVI	ATCGAT	1 cut(s)	2853
BsiHKAI	GWGCWC	4 cut(s)	25, 1477, 1590, 1861
BsiHKCI	CYCGRG	1 cut(s)	2640
BsiSI	CCGG	4 cut(s)	50, 191, 1532, 2936
BslFI	GGGAC	6 cut(s)	436, 914, 1303, 1375, 1480, 2501
BslI	CCNNNNNNNGG	4 cut(s)	379, 1845, 2158, 3036
BsmAI	GTCTC	7 cut(s)	345, 1140, 2018, 2096, 2637, 2833, 2834
BsmFI	GGGAC	6 cut(s)	436, 914, 1303, 1375, 1480, 2501
BsmI	GAATGC	3 cut(s)	145, 520, 2325
BsnI	GGCC	9 cut(s)	594, 645, 1531, 1983, 2428, 2527, 2688, 2935, 2984
Bso31I	GGTCTC	3 cut(s)	345, 2018, 2834
BsoBI	CYCGRG	1 cut(s)	2640
Bsp120I	GGGCCC	1 cut(s)	2525
Bsp1286I	GDGCHC	6 cut(s)	25, 1477, 1590, 1861, 2529, 2748
Bsp1407I	TGTACA	2 cut(s)	2167, 2928
Bsp19I	CCATGG	3 cut(s)	2008, 2283, 2529
BspANI	GGCC	9 cut(s)	594, 645, 1531, 1983, 2428, 2527, 2688, 2935, 2984
BspCNI	CTCAG	6 cut(s)	735, 1363, 1414, 1962, 2028, 2092
BspDI	ATCGAT	1 cut(s)	2853
BspMAI	CTGCAG	1 cut(s)	2110
BspMI	ACCTGC	2 cut(s)	573, 1057
BspPI	GGATC	9 cut(s)	798, 1439, 1599, 1612, 1634, 2222, 2235, 2597, 2858
BspQI	GCTCTTC	1 cut(s)	2996
BspT107I	GGYRCC	3 cut(s)	46, 1045, 2339
BspTNI	GGTCTC	3 cut(s)	345, 2018, 2834
BsrDI	GCAATG	7 cut(s)	484, 866, 2871, 2875, 2891, 2946, 2958
BsrFI	RCCGGY	1 cut(s)	2935
BsrGI	TGTACA	2 cut(s)	2167, 2928
BsrI	ACTGG	4 cut(s)	1493, 1984, 2356, 2559
BssAI	RCCGGY	1 cut(s)	2935
BssNAI	GTATAC	1 cut(s)	1668
BssSI	CACGAG	1 cut(s)	1833
BssT1I	CCWWGG	5 cut(s)	483, 1258, 2008, 2283, 2529
Bst1107I	GTATAC	1 cut(s)	1668
Bst2BI	CACGAG	1 cut(s)	1833
Bst2UI	CCWGG	6 cut(s)	255, 1241, 2078, 2321, 2607, 2766
Bst6I	CTCTTC	2 cut(s)	2550, 2996
BstAPI	GCANNNNNTGC	1 cut(s)	2752
BstAUI	TGTACA	2 cut(s)	2167, 2928
BstBAI	YACGTR	3 cut(s)	1578, 1698, 2749
BstDSI	CCRYGG	4 cut(s)	2008, 2283, 2401, 2529
BstMAI	GTCTC	7 cut(s)	345, 1140, 2018, 2096, 2637, 2833, 2834
BstNI	CCWGG	6 cut(s)	255, 1241, 2078, 2321, 2607, 2766
BstNSI	RCATGY	5 cut(s)	763, 862, 1274, 1631, 2679
BstPAI	GACNNNNGTC	1 cut(s)	2010
BstSCI	CCNGG	7 cut(s)	253, 1239, 1531, 2076, 2319, 2605, 2764
BstSFI	CTRYAG	5 cut(s)	681, 1686, 1940, 2106, 2304
BstSLI	GKGCMC	2 cut(s)	2529, 2748
BstSNI	TACGTA	1 cut(s)	1698
BstV1I	GCAGC	9 cut(s)	174, 571, 1618, 2009, 2505, 2567, 2796, 2799, 2950
BstV2I	GAAGAC	3 cut(s)	413, 2010, 3013
BstX2I	RGATCY	3 cut(s)	1444, 1604, 2227
BstYI	RGATCY	3 cut(s)	1444, 1604, 2227
BstZ17I	GTATAC	1 cut(s)	1668
Bsu15I	ATCGAT	1 cut(s)	2853
BsuRI	GGCC	9 cut(s)	594, 645, 1531, 1983, 2428, 2527, 2688, 2935, 2984
BsuTUI	ATCGAT	1 cut(s)	2853
BtgI	CCRYGG	4 cut(s)	2008, 2283, 2401, 2529
BtsI	GCAGTG	2 cut(s)	204, 581
BtsIMutI	CAGTG	7 cut(s)	204, 581, 1040, 1156, 1299, 1949, 2349
BveI	ACCTGC	2 cut(s)	573, 1057
CaiI	CAGNNNCTG	2 cut(s)	2022, 2129
Cfr10I	RCCGGY	1 cut(s)	2935
Cfr13I	GGNCC	6 cut(s)	928, 1012, 2287, 2525, 2526, 3056
ClaI	ATCGAT	1 cut(s)	2853
CseI	GACGC	1 cut(s)	1310
CspCI	CAANNNNNGTGG	2 cut(s)	1150, 1185
DraI	TTTAAA	4 cut(s)	432, 1218, 1429, 2509
DraIII	CACNNNGTG	1 cut(s)	2752
DriI	GACNNNNNGTC	1 cut(s)	506
EaeI	YGGCCR	1 cut(s)	1981
Eam1104I	CTCTTC	2 cut(s)	2550, 2996
Eam1105I	GACNNNNNGTC	1 cut(s)	506
EarI	CTCTTC	2 cut(s)	2550, 2996
Ecl136II	GAGCTC	2 cut(s)	23, 1588
Eco105I	TACGTA	1 cut(s)	1698
Eco130I	CCWWGG	5 cut(s)	483, 1258, 2008, 2283, 2529
Eco147I	AGGCCT	1 cut(s)	2428
Eco24I	GRGCYC	3 cut(s)	25, 1590, 2529
Eco31I	GGTCTC	3 cut(s)	345, 2018, 2834
Eco32I	GATATC	2 cut(s)	1761, 2761
Eco47I	GGWCC	4 cut(s)	928, 1012, 2287, 3056
Eco53kI	GAGCTC	2 cut(s)	23, 1588
Eco57I	CTGAAG	3 cut(s)	1059, 3016, 3027
Eco72I	CACGTG	2 cut(s)	1578, 2749
Eco88I	CYCGRG	1 cut(s)	2640
EcoICRI	GAGCTC	2 cut(s)	23, 1588
EcoO109I	RGGNCCY	2 cut(s)	928, 1012
EcoRI	GAATTC	1 cut(s)	1264
EcoRII	CCWGG	6 cut(s)	253, 1239, 2076, 2319, 2605, 2764
EcoRV	GATATC	2 cut(s)	1761, 2761
EcoT14I	CCWWGG	5 cut(s)	483, 1258, 2008, 2283, 2529
EcoT22I	ATGCAT	3 cut(s)	522, 1769, 2677
EcoT38I	GRGCYC	3 cut(s)	25, 1590, 2529
ErhI	CCWWGG	5 cut(s)	483, 1258, 2008, 2283, 2529
FalI	AAGNNNNNCTT	4 cut(s)	952, 984, 1348, 1380
FaqI	GGGAC	6 cut(s)	436, 914, 1303, 1375, 1480, 2501
FauI	CCCGC	3 cut(s)	353, 893, 2735
FauNDI	CATATG	2 cut(s)	1228, 2905
FbaI	TGATCA	1 cut(s)	1933
FblI	GTMKAC	3 cut(s)	631, 1099, 1667
FriOI	GRGCYC	3 cut(s)	25, 1590, 2529
GsaI	CCCAGC	1 cut(s)	69
GsuI	CTGGAG	3 cut(s)	302, 2099, 2373
HaeIII	GGCC	9 cut(s)	594, 645, 1531, 1983, 2428, 2527, 2688, 2935, 2984
HapII	CCGG	4 cut(s)	50, 191, 1532, 2936
HgaI	GACGC	1 cut(s)	1310
HincII	GTYRAC	2 cut(s)	2347, 2482
HindII	GTYRAC	2 cut(s)	2347, 2482
HindIII	AAGCTT	2 cut(s)	353, 366
HinfI	GANTC	8 cut(s)	820, 1235, 1328, 1405, 1480, 1953, 1989, 2539
HpaII	CCGG	4 cut(s)	50, 191, 1532, 2936
HphI	GGTGA	2 cut(s)	125, 1019
Hpy166II	GTNNAC	8 cut(s)	632, 956, 1100, 1555, 1668, 2347, 2482, 2929
Hpy8I	GTNNAC	8 cut(s)	632, 956, 1100, 1555, 1668, 2347, 2482, 2929
Hpy99I	CGWCG	1 cut(s)	501
HpyCH4IV	ACGT	4 cut(s)	634, 1577, 1697, 2748
HpySE526I	ACGT	4 cut(s)	634, 1577, 1697, 2748
KpnI	GGTACC	2 cut(s)	1049, 2343
KroI	GCCGGC	1 cut(s)	2935
KroNI	GCCGGC	1 cut(s)	2937
Ksp22I	TGATCA	1 cut(s)	1933
LguI	GCTCTTC	1 cut(s)	2996
LmnI	GCTCC	4 cut(s)	162, 1585, 2190, 2405
Lsp1109I	GCAGC	9 cut(s)	174, 571, 1618, 2009, 2505, 2567, 2796, 2799, 2950
MaeII	ACGT	4 cut(s)	634, 1577, 1697, 2748
MaeIII	GTNAC	3 cut(s)	1975, 2198, 2219
MfeI	CAATTG	1 cut(s)	1776
MflI	RGATCY	3 cut(s)	1444, 1604, 2227
MhlI	GDGCHC	6 cut(s)	25, 1477, 1590, 1861, 2529, 2748
MlyI	GAGTC	3 cut(s)	1414, 1983, 2533
MmeI	TCCRAC	5 cut(s)	243, 780, 969, 2118, 2139
Mph1103I	ATGCAT	3 cut(s)	522, 1769, 2677
MroNI	GCCGGC	1 cut(s)	2935
MroXI	GAANNNNTTC	1 cut(s)	1072
MspA1I	CMGCKG	3 cut(s)	562, 2019, 2716
MspI	CCGG	4 cut(s)	50, 191, 1532, 2936
MspR9I	CCNGG	7 cut(s)	255, 1241, 1533, 2078, 2321, 2607, 2766
MunI	CAATTG	1 cut(s)	1776
Mva1269I	GAATGC	3 cut(s)	145, 520, 2325
MvaI	CCWGG	6 cut(s)	255, 1241, 2078, 2321, 2607, 2766
NaeI	GCCGGC	1 cut(s)	2937
NciI	CCSGG	1 cut(s)	1533
NcoI	CCATGG	3 cut(s)	2008, 2283, 2529
NdeI	CATATG	2 cut(s)	1228, 2905
NgoMIV	GCCGGC	1 cut(s)	2935
NmeAIII	GCCGAG	2 cut(s)	2009, 2071
NmuCI	GTSAC	2 cut(s)	2198, 2219
NsiI	ATGCAT	3 cut(s)	522, 1769, 2677
NspI	RCATGY	5 cut(s)	763, 862, 1274, 1631, 2679
PaeI	GCATGC	2 cut(s)	1274, 2679
PaeR7I	CTCGAG	1 cut(s)	2640
PceI	AGGCCT	1 cut(s)	2428
PciI	ACATGT	1 cut(s)	858
PciSI	GCTCTTC	1 cut(s)	2996
PcsI	WCGNNNNNNNCGW	1 cut(s)	1414
PctI	GAATGC	3 cut(s)	145, 520, 2325
PdiI	GCCGGC	1 cut(s)	2937
PdmI	GAANNNNTTC	1 cut(s)	1072
PfeI	GAWTC	5 cut(s)	820, 1235, 1328, 1480, 1953
PflFI	GACNNNGTC	1 cut(s)	2217
PflMI	CCANNNNNTGG	1 cut(s)	2158
PfoI	TCCNGGA	1 cut(s)	2605
PleI	GAGTC	3 cut(s)	1413, 1983, 2533
PmaCI	CACGTG	2 cut(s)	1578, 2749
PmlI	CACGTG	2 cut(s)	1578, 2749
PpsI	GAGTC	3 cut(s)	1413, 1983, 2533
Ppu21I	YACGTR	3 cut(s)	1578, 1698, 2749
PpuMI	RGGWCCY	2 cut(s)	928, 1012
PscI	ACATGT	1 cut(s)	858
PshAI	GACNNNNGTC	1 cut(s)	2010
PshBI	ATTAAT	1 cut(s)	1002
PsiI	TTATAA	1 cut(s)	1613
Psp124BI	GAGCTC	2 cut(s)	25, 1590
Psp5II	RGGWCCY	2 cut(s)	928, 1012
Psp6I	CCWGG	6 cut(s)	253, 1239, 2076, 2319, 2605, 2764
PspCI	CACGTG	2 cut(s)	1578, 2749
PspFI	CCCAGC	1 cut(s)	65
PspGI	CCWGG	6 cut(s)	253, 1239, 2076, 2319, 2605, 2764
PspOMI	GGGCCC	1 cut(s)	2525
PspPI	GGNCC	6 cut(s)	928, 1012, 2287, 2525, 2526, 3056
PspPPI	RGGWCCY	2 cut(s)	928, 1012
PstI	CTGCAG	1 cut(s)	2110
PstNI	CAGNNNCTG	2 cut(s)	2022, 2129
PsuI	RGATCY	3 cut(s)	1444, 1604, 2227
PsyI	GACNNNGTC	1 cut(s)	2217
SacI	GAGCTC	2 cut(s)	25, 1590
SapI	GCTCTTC	1 cut(s)	2996
Sau96I	GGNCC	6 cut(s)	928, 1012, 2287, 2525, 2526, 3056
ScaI	AGTACT	1 cut(s)	2563
SchI	GAGTC	3 cut(s)	1414, 1983, 2533
ScrFI	CCNGG	7 cut(s)	255, 1241, 1533, 2078, 2321, 2607, 2766
SduI	GDGCHC	6 cut(s)	25, 1477, 1590, 1861, 2529, 2748
SfcI	CTRYAG	5 cut(s)	681, 1686, 1940, 2106, 2304
Sfr274I	CTCGAG	1 cut(s)	2640
SinI	GGWCC	4 cut(s)	928, 1012, 2287, 3056
SlaI	CTCGAG	1 cut(s)	2640
SmiI	ATTTAAAT	1 cut(s)	1218
SmlI	CTYRAG	4 cut(s)	350, 882, 2333, 2640
SmoI	CTYRAG	4 cut(s)	350, 882, 2333, 2640
SnaBI	TACGTA	1 cut(s)	1698
SphI	GCATGC	2 cut(s)	1274, 2679
SseBI	AGGCCT	1 cut(s)	2428
SstI	GAGCTC	2 cut(s)	25, 1590
StuI	AGGCCT	1 cut(s)	2428
StyD4I	CCNGG	7 cut(s)	253, 1239, 1531, 2076, 2319, 2605, 2764
StyI	CCWWGG	5 cut(s)	483, 1258, 2008, 2283, 2529
SwaI	ATTTAAAT	1 cut(s)	1218
TaiI	ACGT	4 cut(s)	637, 1580, 1700, 2751
TaqI	TCGA	2 cut(s)	2641, 2853
TaqII	GACCGA	1 cut(s)	2858
TatI	WGTACW	4 cut(s)	2167, 2561, 2648, 2928
TauI	GCSGC	2 cut(s)	2022, 2691
TfiI	GAWTC	5 cut(s)	820, 1235, 1328, 1480, 1953
TscAI	CASTG	7 cut(s)	211, 581, 1040, 1156, 1299, 1949, 2356
TseFI	GTSAC	2 cut(s)	2198, 2219
TseI	GCWGC	9 cut(s)	187, 559, 1631, 2022, 2493, 2555, 2784, 2787, 2963
Tsp45I	GTSAC	2 cut(s)	2198, 2219
TspGWI	ACGGA	2 cut(s)	1550, 2418
TspRI	CASTG	7 cut(s)	211, 581, 1040, 1156, 1299, 1949, 2356
Tth111I	GACNNNGTC	1 cut(s)	2217
Van91I	CCANNNNNTGG	1 cut(s)	2158
VpaK11BI	GGWCC	4 cut(s)	928, 1012, 2287, 3056
VspI	ATTAAT	1 cut(s)	1002
XapI	RAATTY	5 cut(s)	28, 1264, 1282, 1805, 2207
XbaI	TCTAGA	1 cut(s)	1564
XceI	RCATGY	5 cut(s)	763, 862, 1274, 1631, 2679
XhoI	CTCGAG	1 cut(s)	2640
XmaJI	CCTAGG	1 cut(s)	1258
XmiI	GTMKAC	3 cut(s)	631, 1099, 1667
XmnI	GAANNNNTTC	1 cut(s)	1072
ZrmI	AGTACT	1 cut(s)	2563
Zsp2I	ATGCAT	3 cut(s)	522, 1769, 2677

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.