pycom16g12150

Squamosa promoter-binding-like protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr16
OG0001572
Physical Location & Seq
Forward (+)
8482945 .. 8484693
1749 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom16g12150.1

Sequence Viewer

Length: 1173 bp
ATGCCTTGGTGGATGGTGCTGATACAACCATTGAAAATGATCGAGCTACAGTGCCTCAGATTCTCCTGCTCTATACCAAATGTTACTGGCCGAGGATTCATTGAGGTTGAAGATCATGGTCTCAGCAGCAGCTTCTTTCCATTTATAGTTGCCGAGCAGGAAGTATGTTCAGAGATTTGTATGCTGGAGGGTGCGATTGAGGGGGCTGAGACTGCCGATGACATCCTAGCAGAACCTGAAAAATTGGAAGCCAAGAACCAAGCGATGGACTTTATAAATGAATTGGGTTGGCTCCTTCATAGAGGTCACACTAAATTTAGACTCGGTCACATGGATCCCAACCTAGATCTATTTCCTTTTAGACGGTTCAGGCTGCTTTTGGAGTTCTCCATGGACCATGATTGGTGTGCTGTAGTGAAGAAACTCTTAGGCATTCTGCTTGAGGGTACCGTTGACGCTGGAGAACATCCTTCTATTGAGCTTGCATTGTTGGATATGAGCCTCCTTCACAGAGCTGTGCGAAGAAAGTGCAGGCCTATGGTGGAACTCTTGTTAAGTTTTGTCCTAGATAAAGAGTTAGGCAAAACAGGATCGGACGATGGGCAACAGGTTGGTGGGGACGGCGATAAATTCTTATTTAAGCCTGATGCAGTTGGGCTGATGGGATTGACTCCACTTCATGTTGCTGCCAGTACTGATGGATGTGAGAACATATTGGATGCCTTAACTGATGATCCTGGAAAGGTGGGAATTAAAGCTTGGGGAAATGCTCGAGACAGTACAGGATTGACACCAAATGATTATGCATGTCAACGAAGCTGCTCCACCTACATCCAAATTGTCCAGAGGAAAATCAGCAAGAAACTTGAAAGCAGGCACTTGGTGCTTGATATCCCTGGCGTTATCTTAGACAGCAGCAGCAAGAAGAAACAATTAGACGGGCATAAGTCTTCAAAAGTCTCCAGCTTGGAGACCGACAGGATTGATATGAAATCAATGCAGGCACATTGCAAGCTATGTGAAATGAAACTAGCATATGGCAACACTAGGTCGCTTGTGTACAGGCCGGCAATGCTGTCAATGGTAGCCATTGCTGCTGTCTGTGTTTGCGCGGCCTTGCTATTCAAAAGCTCACCTGTCACGTGGGAACGCTTGAAGTATGGAACTAGCTAA

Protein Analysis

391

Amino Acids

43.35

Weight (kDa)

6.03

Isoelectric Point (pI)

36.11

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Ig_SPL7 PF26102 14 - 61 2.2e-20 SPL7-like, immunoglobulin-like domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
Loading...
Style Settings
Image
Tree File
Tip: Beautify your tree with professional tools

Download the Full Tree (.nwk) file, then upload it to any of the following tools to customize colors, fonts, annotations, clades, and branch support.

Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 275
Acc65I GGTACC 1 cut(s) 446
AccB1I GGYRCC 1 cut(s) 446
AccB7I CCANNNNNTGG 1 cut(s) 265
AccII CGCG 1 cut(s) 1112
AciI CCGC 1 cut(s) 1112
AclWI GGATC 4 cut(s) 329, 342, 598, 728
AcoI YGGCCR 1 cut(s) 88
AcsI RAATTY 2 cut(s) 314, 629
AcvI CACGTG 1 cut(s) 1143
AdeI CACNNNGTG 1 cut(s) 883
AfaI GTAC 4 cut(s) 448, 694, 781, 1061
AfiI CCNNNNNNNGG 1 cut(s) 265
AgsI TTSAA 6 cut(s) 34, 110, 869, 954, 1126, 1156
AjnI CCWGG 2 cut(s) 736, 895
AjuI GAANNNNNNNTTGG 2 cut(s) 742, 774
Alw26I GTCTC 5 cut(s) 125, 203, 768, 964, 965
AlwI GGATC 4 cut(s) 329, 342, 598, 728
AlwNI CAGNNNCTG 1 cut(s) 236
Ama87I CYCGRG 1 cut(s) 771
AoxI GGCC 4 cut(s) 88, 533, 1064, 1113
ApeKI GCWGC 8 cut(s) 126, 129, 373, 686, 819, 915, 918, 1094
ApoI RAATTY 2 cut(s) 314, 629
Asp718I GGTACC 1 cut(s) 446
AspLEI GCGC 1 cut(s) 1112
AspS9I GGNCC 1 cut(s) 394
AsuHPI GGTGA 1 cut(s) 1125
AvaI CYCGRG 1 cut(s) 771
AvaII GGWCC 1 cut(s) 394
BamHI GGATCC 1 cut(s) 334
BanI GGYRCC 1 cut(s) 446
BbrPI CACGTG 1 cut(s) 1143
BbsI GAAGAC 1 cut(s) 942
BbvI GCAGC 8 cut(s) 138, 141, 360, 673, 806, 927, 930, 1081
BccI CCATC 5 cut(s) 7, 259, 593, 655, 692
BceAI ACGGC 1 cut(s) 637
BcgI CGANNNNNNTGC 2 cut(s) 510, 544
BciT130I CCWGG 2 cut(s) 738, 897
BcoDI GTCTC 5 cut(s) 125, 203, 768, 964, 965
BfaI CTAG 6 cut(s) 227, 344, 566, 1031, 1047, 1167
BfmI CTRYAG 2 cut(s) 47, 411
BglII AGATCT 1 cut(s) 346
BisI GCNGC 9 cut(s) 127, 130, 374, 687, 820, 916, 919, 1095, 1113
BlsI GCNGC 9 cut(s) 128, 131, 375, 688, 821, 917, 920, 1096, 1114
BmcAI AGTACT 1 cut(s) 694
Bme1390I CCNGG 2 cut(s) 738, 897
Bme18I GGWCC 1 cut(s) 394
BmeT110I CYCGRG 1 cut(s) 771
BmgT120I GGNCC 1 cut(s) 394
BmiI GGNNCC 3 cut(s) 293, 336, 448
BmrFI CCNGG 2 cut(s) 738, 897
BmsI GCATC 2 cut(s) 637, 709
BpiI GAAGAC 1 cut(s) 942
BpmI CTGGAG 3 cut(s) 206, 480, 946
BpuEI CTTGAG 1 cut(s) 461
BsaAI YACGTR 1 cut(s) 1143
BsaI GGTCTC 2 cut(s) 125, 965
BsaJI CCNNGG 4 cut(s) 5, 91, 390, 895
Bsc4I CCNNNNNNNGG 1 cut(s) 265
Bse118I RCCGGY 1 cut(s) 1066
Bse1I ACTGG 2 cut(s) 91, 690
Bse3DI GCAATG 3 cut(s) 1006, 1077, 1089
BseBI CCWGG 2 cut(s) 738, 897
BseDI CCNNGG 4 cut(s) 5, 91, 390, 895
BseGI GGATG 6 cut(s) 18, 222, 466, 707, 724, 831
BseLI CCNNNNNNNGG 1 cut(s) 265
BseMI GCAATG 3 cut(s) 1006, 1077, 1089
BseMII CTCAG 3 cut(s) 70, 136, 198
BseNI ACTGG 2 cut(s) 91, 690
BseXI GCAGC 8 cut(s) 138, 141, 360, 673, 806, 927, 930, 1081
BsgI GTGCAG 1 cut(s) 550
Bsh1236I CGCG 1 cut(s) 1112
BshFI GGCC 4 cut(s) 90, 535, 1066, 1115
BshNI GGYRCC 1 cut(s) 446
BsiHKCI CYCGRG 1 cut(s) 771
BsiSI CCGG 1 cut(s) 1067
BslFI GGGAC 1 cut(s) 632
BslI CCNNNNNNNGG 1 cut(s) 265
BsmAI GTCTC 5 cut(s) 125, 203, 768, 964, 965
BsmFI GGGAC 1 cut(s) 632
BsmI GAATGC 1 cut(s) 432
BsnI GGCC 4 cut(s) 90, 535, 1066, 1115
Bso31I GGTCTC 2 cut(s) 125, 965
BsoBI CYCGRG 1 cut(s) 771
Bsp1407I TGTACA 1 cut(s) 1059
Bsp143I GATC 6 cut(s) 39, 112, 334, 346, 590, 733
Bsp19I CCATGG 1 cut(s) 390
BspACI CCGC 1 cut(s) 1112
BspANI GGCC 4 cut(s) 90, 535, 1066, 1115
BspCNI CTCAG 3 cut(s) 69, 135, 199
BspFNI CGCG 1 cut(s) 1112
BspLI GGNNCC 3 cut(s) 293, 336, 448
BspPI GGATC 4 cut(s) 329, 342, 598, 728
BspT107I GGYRCC 1 cut(s) 446
BspTNI GGTCTC 2 cut(s) 125, 965
BsrDI GCAATG 3 cut(s) 1006, 1077, 1089
BsrFI RCCGGY 1 cut(s) 1066
BsrGI TGTACA 1 cut(s) 1059
BsrI ACTGG 2 cut(s) 91, 690
BssAI RCCGGY 1 cut(s) 1066
BssECI CCNNGG 4 cut(s) 5, 91, 390, 895
BssMI GATC 6 cut(s) 39, 112, 334, 346, 590, 733
BssT1I CCWWGG 2 cut(s) 5, 390
Bst2UI CCWGG 2 cut(s) 738, 897
Bst4CI ACNGT 4 cut(s) 51, 366, 451, 779
BstAPI GCANNNNNTGC 1 cut(s) 883
BstAUI TGTACA 1 cut(s) 1059
BstBAI YACGTR 1 cut(s) 1143
BstC8I GCNNGC 6 cut(s) 483, 533, 875, 1002, 1013, 1068
BstDEI CTNAG 5 cut(s) 56, 122, 207, 427, 907
BstDSI CCRYGG 1 cut(s) 390
BstF5I GGATG 6 cut(s) 18, 222, 466, 707, 724, 831
BstFNI CGCG 1 cut(s) 1112
BstHHI GCGC 1 cut(s) 1112
BstKTI GATC 6 cut(s) 42, 115, 337, 349, 593, 736
BstMAI GTCTC 5 cut(s) 125, 203, 768, 964, 965
BstMBI GATC 6 cut(s) 39, 112, 334, 346, 590, 733
BstMWI GCNNNNNNNGC 4 cut(s) 212, 883, 1072, 1094
BstNI CCWGG 2 cut(s) 738, 897
BstNSI RCATGY 1 cut(s) 810
BstSCI CCNGG 2 cut(s) 736, 895
BstSFI CTRYAG 2 cut(s) 47, 411
BstUI CGCG 1 cut(s) 1112
BstV1I GCAGC 8 cut(s) 138, 141, 360, 673, 806, 927, 930, 1081
BstV2I GAAGAC 1 cut(s) 942
BstX2I RGATCY 2 cut(s) 334, 346
BstYI RGATCY 2 cut(s) 334, 346
BsuRI GGCC 4 cut(s) 90, 535, 1066, 1115
BtgI CCRYGG 1 cut(s) 390
BtgZI GCGATG 1 cut(s) 278
BtsCI GGATG 6 cut(s) 18, 222, 466, 707, 724, 831
BtsIMutI CAGTG 1 cut(s) 56
Cac8I GCNNGC 6 cut(s) 483, 533, 875, 1002, 1013, 1068
CaiI CAGNNNCTG 1 cut(s) 236
CfoI GCGC 1 cut(s) 1112
Cfr10I RCCGGY 1 cut(s) 1066
Cfr13I GGNCC 1 cut(s) 394
CseI GACGC 1 cut(s) 464
Csp6I GTAC 4 cut(s) 447, 693, 780, 1060
CviAII CATG 6 cut(s) 116, 331, 391, 398, 680, 807
CviQI GTAC 4 cut(s) 447, 693, 780, 1060
DdeI CTNAG 5 cut(s) 56, 122, 207, 427, 907
DpnI GATC 6 cut(s) 41, 114, 336, 348, 592, 735
DpnII GATC 6 cut(s) 39, 112, 334, 346, 590, 733
DraIII CACNNNGTG 1 cut(s) 883
EaeI YGGCCR 1 cut(s) 88
Eco130I CCWWGG 2 cut(s) 5, 390
Eco147I AGGCCT 1 cut(s) 535
Eco31I GGTCTC 2 cut(s) 125, 965
Eco32I GATATC 1 cut(s) 892
Eco47I GGWCC 1 cut(s) 394
Eco72I CACGTG 1 cut(s) 1143
Eco88I CYCGRG 1 cut(s) 771
EcoRII CCWGG 2 cut(s) 736, 895
EcoRV GATATC 1 cut(s) 892
EcoT14I CCWWGG 2 cut(s) 5, 390
EcoT22I ATGCAT 1 cut(s) 808
ErhI CCWWGG 2 cut(s) 5, 390
FaeI CATG 6 cut(s) 119, 334, 394, 401, 683, 810
FalI AAGNNNNNCTT 2 cut(s) 410, 442
FaqI GGGAC 1 cut(s) 632
FatI CATG 6 cut(s) 115, 330, 390, 397, 679, 806
FauNDI CATATG 1 cut(s) 1036
Fnu4HI GCNGC 9 cut(s) 127, 130, 374, 687, 820, 916, 919, 1095, 1113
FokI GGATG 6 cut(s) 25, 209, 453, 714, 731, 818
Fsp4HI GCNGC 9 cut(s) 127, 130, 374, 687, 820, 916, 919, 1095, 1113
FspBI CTAG 6 cut(s) 227, 344, 566, 1031, 1047, 1167
GlaI GCGC 1 cut(s) 1111
GluI GCNGC 9 cut(s) 127, 130, 374, 687, 820, 916, 919, 1095, 1113
GsuI CTGGAG 3 cut(s) 206, 480, 946
HaeIII GGCC 4 cut(s) 90, 535, 1066, 1115
HapII CCGG 1 cut(s) 1067
HgaI GACGC 1 cut(s) 464
HhaI GCGC 1 cut(s) 1112
Hin1II CATG 6 cut(s) 119, 334, 394, 401, 683, 810
Hin6I GCGC 1 cut(s) 1110
HinP1I GCGC 1 cut(s) 1110
HincII GTYRAC 2 cut(s) 454, 812
HindII GTYRAC 2 cut(s) 454, 812
HindIII AAGCTT 1 cut(s) 756
HinfI GANTC 4 cut(s) 60, 96, 321, 670
HpaII CCGG 1 cut(s) 1067
HphI GGTGA 1 cut(s) 1125
Hpy166II GTNNAC 3 cut(s) 454, 812, 1060
Hpy188I TCNGA 3 cut(s) 59, 172, 595
Hpy188III TCNNGA 2 cut(s) 773, 844
Hpy8I GTNNAC 3 cut(s) 454, 812, 1060
HpyAV CCTTC 3 cut(s) 305, 480, 515
HpyCH4III ACNGT 4 cut(s) 51, 366, 451, 779
HpyCH4IV ACGT 1 cut(s) 1142
HpyCH4V TGCA 6 cut(s) 485, 531, 650, 806, 1000, 1011
HpyF10VI GCNNNNNNNGC 4 cut(s) 212, 883, 1072, 1094
HpyF3I CTNAG 5 cut(s) 56, 122, 207, 427, 907
HpySE526I ACGT 1 cut(s) 1142
Hsp92II CATG 6 cut(s) 119, 334, 394, 401, 683, 810
HspAI GCGC 1 cut(s) 1110
KpnI GGTACC 1 cut(s) 450
KroI GCCGGC 1 cut(s) 1066
KroNI GCCGGC 1 cut(s) 1068
Kzo9I GATC 6 cut(s) 39, 112, 334, 346, 590, 733
LmnI GCTCC 2 cut(s) 297, 827
Lsp1109I GCAGC 8 cut(s) 138, 141, 360, 673, 806, 927, 930, 1081
LweI GCATC 2 cut(s) 637, 709
MaeI CTAG 6 cut(s) 227, 344, 566, 1031, 1047, 1167
MaeII ACGT 1 cut(s) 1142
MaeIII GTNAC 4 cut(s) 82, 305, 326, 1138
MalI GATC 6 cut(s) 41, 114, 336, 348, 592, 735
MboI GATC 6 cut(s) 39, 112, 334, 346, 590, 733
MboII GAAGA 5 cut(s) 122, 430, 534, 937, 942
MflI RGATCY 2 cut(s) 334, 346
MluCI AATT 7 cut(s) 242, 281, 314, 629, 750, 837, 932
MlyI GAGTC 2 cut(s) 315, 664
MmeI TCCRAC 1 cut(s) 471
MnlI CCTC 9 cut(s) 65, 86, 97, 181, 193, 296, 436, 512, 840
Mph1103I ATGCAT 1 cut(s) 808
MroNI GCCGGC 1 cut(s) 1066
MseI TTAA 4 cut(s) 554, 639, 725, 753
MspI CCGG 1 cut(s) 1067
MspR9I CCNGG 2 cut(s) 738, 897
Mva1269I GAATGC 1 cut(s) 432
MvaI CCWGG 2 cut(s) 738, 897
MvnI CGCG 1 cut(s) 1112
MwoI GCNNNNNNNGC 4 cut(s) 212, 883, 1072, 1094
NaeI GCCGGC 1 cut(s) 1068
NcoI CCATGG 1 cut(s) 390
NdeI CATATG 1 cut(s) 1036
NdeII GATC 6 cut(s) 39, 112, 334, 346, 590, 733
NgoMIV GCCGGC 1 cut(s) 1066
NlaIII CATG 6 cut(s) 119, 334, 394, 401, 683, 810
NlaIV GGNNCC 3 cut(s) 293, 336, 448
NmeAIII GCCGAG 2 cut(s) 116, 178
NmuCI GTSAC 3 cut(s) 305, 326, 1138
NsiI ATGCAT 1 cut(s) 808
NspI RCATGY 1 cut(s) 810
PaeR7I CTCGAG 1 cut(s) 771
PceI AGGCCT 1 cut(s) 535
PctI GAATGC 1 cut(s) 432
PdiI GCCGGC 1 cut(s) 1068
PfeI GAWTC 2 cut(s) 60, 96
PflFI GACNNNGTC 1 cut(s) 324
PflMI CCANNNNNTGG 1 cut(s) 265
PfoI TCCNGGA 1 cut(s) 736
PkrI GCNGC 9 cut(s) 128, 131, 375, 688, 821, 917, 920, 1096, 1114
PleI GAGTC 2 cut(s) 315, 664
PmaCI CACGTG 1 cut(s) 1143
PmlI CACGTG 1 cut(s) 1143
PpsI GAGTC 2 cut(s) 315, 664
Ppu21I YACGTR 1 cut(s) 1143
PsiI TTATAA 1 cut(s) 275
Psp6I CCWGG 2 cut(s) 736, 895
PspCI CACGTG 1 cut(s) 1143
PspGI CCWGG 2 cut(s) 736, 895
PspN4I GGNNCC 3 cut(s) 293, 336, 448
PspPI GGNCC 1 cut(s) 394
PstNI CAGNNNCTG 1 cut(s) 236
PsuI RGATCY 2 cut(s) 334, 346
PsyI GACNNNGTC 1 cut(s) 324
RsaI GTAC 4 cut(s) 448, 694, 781, 1061
RsaNI GTAC 4 cut(s) 447, 693, 780, 1060
SaqAI TTAA 4 cut(s) 554, 639, 725, 753
SatI GCNGC 9 cut(s) 127, 130, 374, 687, 820, 916, 919, 1095, 1113
Sau3AI GATC 6 cut(s) 39, 112, 334, 346, 590, 733
Sau96I GGNCC 1 cut(s) 394
ScaI AGTACT 1 cut(s) 694
SchI GAGTC 2 cut(s) 315, 664
ScrFI CCNGG 2 cut(s) 738, 897
SfaNI GCATC 2 cut(s) 637, 709
SfcI CTRYAG 2 cut(s) 47, 411
Sfr274I CTCGAG 1 cut(s) 771
SinI GGWCC 1 cut(s) 394
SlaI CTCGAG 1 cut(s) 771
SmlI CTYRAG 2 cut(s) 440, 771
SmoI CTYRAG 2 cut(s) 440, 771
Sse9I AATT 7 cut(s) 242, 281, 314, 629, 750, 837, 932
SseBI AGGCCT 1 cut(s) 535
SsiI CCGC 1 cut(s) 1112
SspMI CTAG 6 cut(s) 227, 344, 566, 1031, 1047, 1167
StuI AGGCCT 1 cut(s) 535
StyD4I CCNGG 2 cut(s) 736, 895
StyI CCWWGG 2 cut(s) 5, 390
TaaI ACNGT 4 cut(s) 51, 366, 451, 779
TaiI ACGT 1 cut(s) 1145
TaqI TCGA 2 cut(s) 42, 772
TaqII GACCGA 2 cut(s) 314, 989
TasI AATT 7 cut(s) 242, 281, 314, 629, 750, 837, 932
TatI WGTACW 3 cut(s) 692, 779, 1059
TauI GCSGC 1 cut(s) 1115
TfiI GAWTC 2 cut(s) 60, 96
Tru1I TTAA 4 cut(s) 554, 639, 725, 753
Tru9I TTAA 4 cut(s) 554, 639, 725, 753
TscAI CASTG 1 cut(s) 56
TseFI GTSAC 3 cut(s) 305, 326, 1138
TseI GCWGC 8 cut(s) 126, 129, 373, 686, 819, 915, 918, 1094
Tsp45I GTSAC 3 cut(s) 305, 326, 1138
TspDTI ATGAA 6 cut(s) 88, 287, 294, 668, 1004, 1040
TspRI CASTG 1 cut(s) 56
Tth111I GACNNNGTC 1 cut(s) 324
Van91I CCANNNNNTGG 1 cut(s) 265
VpaK11BI GGWCC 1 cut(s) 394
XapI RAATTY 2 cut(s) 314, 629
XceI RCATGY 1 cut(s) 810
XhoI CTCGAG 1 cut(s) 771
XspI CTAG 6 cut(s) 227, 344, 566, 1031, 1047, 1167
ZrmI AGTACT 1 cut(s) 694
Zsp2I ATGCAT 1 cut(s) 808
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.