Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr16

Orthogroup ID

OG0001572

Physical Location & Seq

Genomic Coordinates Forward (+)

10533886 .. 10540527

Gene Structure 6642 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

MD16G1137600.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 3090 bp

ATGGAGGCTTTCGGAGCCGGATCTCGGAAGTTCTCTAGTCCGACGGTGTCGGATTTGAAGGCGGTTGGGAAAAAGAGTGTGGAGTGGGATTTAAATGATTGGAAATGGGATGGTGATCTTTTTACTGCTAGTCCTTTGAATGCTGCACCATCTGATTGTAGGAGTAGGCAGTTGTTTCCGCTTGGGCCGCCGGAAACTCCCTCCACTGCTGGTTTGTCCAACACTTCTTCTTCTGGTTCGGATGACATCTGTCCGGGGAATGAGAAAGGTAAGAGAGAGCTGGAGAAAAGAAGAAGGGATAGTATTGTAGAAAATGTAGACTTAAACAATGAAGTTGGGTCTCTTAATCTCAAGCTTGGCGAGCAAGCTTACCCAATCATGGAAGGAGAGGTACAAACTGGGAAGAAGACGAAGATAGTTGGGACTACTTTAAACCGGGCAGTTTGTCAGGTGGAGGACTGTAAGGCTGATCTTAGCAATGCCAAGGATTACCACCGACGACATAAGGTCTGTGATATGCATTCTAAGGCAACTAAAGCGCCGGTGGGAAATGTTTTGCAGCGGTTCTGTCAGCAGTGTAGCAGGTTTCATGTTCTTCAAGAGTTTGATGAAGGAAGGAGAAGTTGTCGTAGGCGTTTGGCTGGCCACAATAGGAGGAGAAGAAAAACACATCCCGATACTGTAGTTAATGAAGGCTCATTGAACGATGAAAGAGGAAGCAGTTATCTATTGATTAGTTTGCTGAGAATACTCTCCAACATGCACTCTAATAGTTCAGATCAAACAAAGGATCAGGATTTGCTTTCTCATCTTTTGAAGAATCTAGCCAATCTTTCTGGTACAGTTGATGGAAGGAACATGTCTGCATTGCTTACGGCATCTCAAGGTTTACTAAATGGTGGGGCATCAATTCAGACTGCACAAAAGGTCCTAGATACAGTTTCCAATGGCTGTGAACCAAGTAAGCCTTCTGTTTCAGCCTCTAAGATGGATGACTATGGTAATCGTGAGGACCCTTCAAGACCTACACAACAGTGTTCTACAGTACCTGCATCAGATTTCAGGATAATATCTTCAGTTGATGCCGATCATGGAGGTCTGCAAGTTGTTTCAGGTGTAAATGCCACCAAGCCATTTCCCTCAAGAGACCGAGTTCCATCCACATCAGTTGCACCAGAAGCTACAACGGGGAGGATCCAGTTAAATGGCATTGATTTAAATAATACATATGACGATTCCCAGGACTATTTGGCCAGCCTAGGGAATTCTCAAGCCCCTGTAAATTCAGGGACTGTGTCTCATGGTTTTCCCTTACGGATGCGGCAGGATTTGCAAAAGTCTAGCCCACCTCAGACAAGTGGGACTTCTTACTCAACTTCATCAAGTTCTAGTGGAGAGGTTCAGAGTCGCACGGATCGTATTGTTTTTAAACTCTTTGGAAAAGATCCAAATGATCTTCCATTTGTTCTACGATCACAGATTCTCGACTGGCTGTCCCACAGTCCCACAGACATTGAAAGTTACATAAGGCCGGGATGTATCATTTTGACAGTTTACCTCCGTCTAGAAAAATCCACGTGGGAGGAGCTGTGCTGTAATCTGGGATCCATTTTGAAACGACTTTTGCATGCTGCCAACGATCCTTTCTGGACAACAGGATGGGTGTATACTAGGGTGCAACATTTTGTAGCATTTACGTACAATGGTCACGTTGTCTTAGACACACCCTTACCTCTGAAAAGCAATAAAAACTGCAGGATATCATGCATCAAACCAATTGCTGTTTCTTTATCACAAAGAGCTGACTTTGCAGTAAAGGGGTTTAACCTTTCTCATGCCACCACAAGGTTGCTCTGTGCTCTGGAAGGGAAGTATCTAGTCCAAGAAACTTGTTATGACTTGGTGGATGGTGCTGATACAACCATTGAAAATGATCAGCTACAGTGCCTCAGATTTTCCTGCTCTATACCAAATGTTACTGGCCGAGGATTCATTGAGGTTGAAGATCATGGTCTCAGCAGCAGCTTCTTTCCATTTATAGTTGCCGAGCAGGAAGTATGTTCAGAGATTTGTATGCTGGAGGGTGCAATTGAGGGGGCTGAGACTGCCGATGACATCCAAGCAGAACCTGAAAAATTGGAAGCCAAGAACCAAGCGATGGACTTTATAAATGAATTGGGTTGGCTCCTTCATAGAGGTCACACTAAATTTAGACTCGGTCACATGGATCCCAAACTAGATCTATTTCCTTTTAGACGGTTCAGGCTGCTTTTGGAGTTCTCCATGGACCATGATTGGTGTGCTGTAGTGAAGAAACTCTTAGGCATTCTGCTTGAGGGTACCGTTGACGCCGGAGAACATCCTTCTATTGAGCTTGCATTGTTGGACATGAGCCTCCTTCACAGAGCTGTGCGAAGAAAGTGCAGGCCTATGGTGGAACTCTTGTTAAGTTTTGTCCTAGATAAAGGGTTAGGCAAAACAGGATCAGAGGACGGCCAACAAGTTGGCGGGGATGGCAATAAATTCTTATTTAAACCTGACGCAGTTGGGCTGATGGGATTGACTCCTCTTCATGTTGCAGCCAGTACTGATGGATGTGAGAACATATTGGATGCCTTAACTGATGATCCCGGAATGGTGGGAATTAAAGCTTGGGGAAATGCTCGAGACAGTACAGGATTGACACCAAATGATTATGCATGTCTGCGAAGCTGCTACACCTACATCCAAATTGTCCAGAGGAAAATCAGCAAGAAACATGAAAGCAGGCACGTGGTGCTTGATATCCCTGGCGTTATCTTAGACAGCAGCAGCAAGAAGAAACAATTAGACAGGCATAGGTCCTCAAAAGTCTCCAGCTTGGAGACCGAAAGGATTGATATGAAAGCAATGCAGGCACATTGCAAGCTATGTGAAATGAAACTAGCTTATGGCAACACTAGGTCGCTTGTGTACAGGCCGGCAATGCTGTCAATGGTAGCCATTGCTGCTGTCTGTGTTTGCGCTGCCTTGCTATTCAAAAGCTCACCTGAAGTCCTTTATATCTTCCAGCCCTTCACGTGGGAACGCTTGAAGTATGGAACCAGCTAA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003700 GO:0006355 GO:0008150 GO:0009889 GO:0010468 GO:0010556 GO:0019219 GO:0019222 GO:0031323 GO:0031326 GO:0050789 GO:0050794 GO:0051171 GO:0051252 GO:0060255 GO:0065007 GO:0080090 GO:0140110 GO:1903506 GO:2000112 GO:2001141

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

SBP

Protein Analysis

1030

Amino Acids

113.61

Weight (kDa)

7.11

Isoelectric Point (pI)

43.26

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
SBP	PF03110	149 - 222	1e-29	SBP domain
Ig_SPL7	PF26102	582 - 692	4.1e-44	SPL7-like, immunoglobulin-like domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

Style Settings

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0001572)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT2G47070 AT2G47070 AT3G60030
fragaria_vesca	FvH4_4g21420 FvH4_4g21420 FvH4_4g21420 FvH4_4g21420 FvH4_4g21420 FvH4_4g21420 FvH4_4g21420 FvH4_4g21420 FvH4_4g21420 FvH4_4g21420 FvH4_4g21420 FvH4_4g21420 FvH4_4g21420 FvH4_4g21420 FvH4_4g21420 FvH4_4g21420
malus_domestica	MD13G1141300.v1.1 MD13G1141400.v1.1 MD16G1137600.v1.1 MD16G1137700.v1.1
prunus_persica	Prupe.1G106500_v2.0.a1 Prupe.1G106500_v2.0.a1 Prupe.1G106600_v2.0.a1
pyrus_communis	pycom13g12280 pycom13g12290 pycom16g12140 pycom16g12150 pycom16g12160
rosa_chinensis	RchiOBHm_Chr4g0427341 RchiOBHm_Chr4g0427351
rosa_laevigata	RLG00000007250 RLG00000007251
rosa_multiflora	Rmu_sc0003813.1_g000017 Rmu_sc0003813.1_g000018
rosa_roxburghii	Rroxscaffold_5G00369020 Rroxscaffold_5G00369030
rosa_rugosa	Rorug04G0216800
rosa_samantha	Rh4AG273100 Rh4AG273300 Rh4BG278800 Rh4BG278900 Rh4CG294100 Rh4CG294200 Rh4DG276200 Rh4DG276300
rosa_wichuraiana	Rw4G023700 Rw4G023710

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	1 cut(s)	2168
Acc36I	ACCTGC	2 cut(s)	573, 1057
Acc65I	GGTACC	1 cut(s)	2339
AccB1I	GGYRCC	1 cut(s)	2339
AccB7I	CCANNNNNTGG	1 cut(s)	2158
AccI	GTMKAC	2 cut(s)	318, 1667
AciI	CCGC	6 cut(s)	62, 179, 188, 562, 1321, 2508
AcoI	YGGCCR	4 cut(s)	643, 1251, 1981, 2494
AcsI	RAATTY	4 cut(s)	1264, 1282, 2207, 2522
AcuI	CTGAAG	2 cut(s)	1059, 3051
AcvI	CACGTG	3 cut(s)	1578, 2773, 3060
AcyI	GRCGYC	1 cut(s)	2349
AdeI	CACNNNGTG	1 cut(s)	2776
AfaI	GTAC	8 cut(s)	393, 841, 1047, 1700, 2341, 2587, 2674, 2954
AfiI	CCNNNNNNNGG	6 cut(s)	24, 379, 1260, 1845, 2158, 3060
AflIII	ACRYGT	1 cut(s)	858
AhdI	GACNNNNNGTC	2 cut(s)	506, 1492
AjnI	CCWGG	2 cut(s)	1239, 2788
AjuI	GAANNNNNNNTTGG	8 cut(s)	952, 984, 1628, 1660, 1768, 1800, 2635, 2667
AleI	CACNNNNGTG	1 cut(s)	1033
Alw21I	GWGCWC	1 cut(s)	1861
Alw26I	GTCTC	8 cut(s)	345, 1140, 1302, 2018, 2096, 2661, 2857, 2858
AlwNI	CAGNNNCTG	3 cut(s)	1049, 1292, 2129
Ama87I	CYCGRG	1 cut(s)	2664
AoxI	GGCC	8 cut(s)	185, 643, 1251, 1529, 1981, 2426, 2494, 2957
ApoI	RAATTY	4 cut(s)	1264, 1282, 2207, 2522
Asp718I	GGTACC	1 cut(s)	2339
AspA2I	CCTAGG	1 cut(s)	1258
AspLEI	GCGC	2 cut(s)	541, 3005
AspS9I	GGNCC	5 cut(s)	185, 928, 1012, 2287, 2841
AsuC2I	CCSGG	4 cut(s)	255, 437, 1533, 2631
AsuHPI	GGTGA	2 cut(s)	125, 3018
AvaI	CYCGRG	1 cut(s)	2664
AvaII	GGWCC	4 cut(s)	928, 1012, 2287, 2841
AvrII	CCTAGG	1 cut(s)	1258
BaeI	ACNNNNGTAYC	2 cut(s)	669, 702
BalI	TGGCCA	2 cut(s)	645, 1253
BamHI	GGATCC	3 cut(s)	1194, 1604, 2227
BanI	GGYRCC	1 cut(s)	2339
BarI	GAAGNNNNNNTAC	2 cut(s)	375, 407
BbrPI	CACGTG	3 cut(s)	1578, 2773, 3060
BbsI	GAAGAC	1 cut(s)	413
Bbv12I	GWGCWC	1 cut(s)	1861
BceAI	ACGGC	2 cut(s)	891, 2509
BcgI	CGANNNNNNTGC	2 cut(s)	2403, 2437
BciT130I	CCWGG	2 cut(s)	1241, 2790
BclI	TGATCA	1 cut(s)	1933
BcnI	CCSGG	4 cut(s)	255, 437, 1533, 2631
BcoDI	GTCTC	8 cut(s)	345, 1140, 1302, 2018, 2096, 2661, 2857, 2858
BfmI	CTRYAG	5 cut(s)	681, 1041, 1753, 1940, 2304
BfoI	RGCGCY	1 cut(s)	542
BfuAI	ACCTGC	2 cut(s)	573, 1057
BglII	AGATCT	1 cut(s)	2239
BlnI	CCTAGG	1 cut(s)	1258
BmcAI	AGTACT	1 cut(s)	2587
Bme1390I	CCNGG	6 cut(s)	255, 437, 1241, 1533, 2631, 2790
Bme18I	GGWCC	4 cut(s)	928, 1012, 2287, 2841
BmeRI	GACNNNNNGTC	2 cut(s)	506, 1492
BmeT110I	CYCGRG	1 cut(s)	2664
BmgT120I	GGNCC	5 cut(s)	185, 928, 1012, 2287, 2841
BmiI	GGNNCC	8 cut(s)	16, 1014, 1196, 1606, 2186, 2229, 2341, 3082
BmrFI	CCNGG	6 cut(s)	255, 437, 1241, 1533, 2631, 2790
BmrI	ACTGGG	1 cut(s)	408
BmsI	GCATC	7 cut(s)	887, 914, 1061, 1072, 1308, 1776, 2602
BmuI	ACTGGG	1 cut(s)	408
BoxI	GACNNNNGTC	1 cut(s)	249
BpiI	GAAGAC	1 cut(s)	413
BpmI	CTGGAG	3 cut(s)	302, 2099, 2839
BpuEI	CTTGAG	5 cut(s)	335, 867, 1126, 1254, 2354
BpuMI	CCSGG	4 cut(s)	255, 437, 1533, 2631
BsaAI	YACGTR	4 cut(s)	1578, 1698, 2773, 3060
BsaBI	GATNNNNATC	2 cut(s)	114, 1086
BsaHI	GRCGYC	1 cut(s)	2349
BsaI	GGTCTC	4 cut(s)	345, 1140, 2018, 2858
BsaJI	CCNNGG	7 cut(s)	254, 483, 1239, 1258, 1984, 2283, 2788
BsaXI	ACNNNNNCTCC	4 cut(s)	610, 640, 1574, 1604
Bsc4I	CCNNNNNNNGG	6 cut(s)	24, 379, 1260, 1845, 2158, 3060
Bse118I	RCCGGY	2 cut(s)	541, 2959
Bse1I	ACTGG	5 cut(s)	403, 1198, 1493, 1984, 2583
Bse3DI	GCAATG	6 cut(s)	484, 866, 2895, 2899, 2970, 2982
Bse8I	GATNNNNATC	2 cut(s)	114, 1086
BseBI	CCWGG	2 cut(s)	1241, 2790
BseDI	CCNNGG	7 cut(s)	254, 483, 1239, 1258, 1984, 2283, 2788
BseJI	GATNNNNATC	2 cut(s)	114, 1086
BseLI	CCNNNNNNNGG	6 cut(s)	24, 379, 1260, 1845, 2158, 3060
BseMI	GCAATG	6 cut(s)	484, 866, 2895, 2899, 2970, 2982
BseMII	CTCAG	5 cut(s)	734, 1364, 1963, 2029, 2091
BseNI	ACTGG	5 cut(s)	403, 1198, 1493, 1984, 2583
BseRI	GAGGAG	3 cut(s)	670, 1598, 2556
BsgI	GTGCAG	3 cut(s)	129, 903, 2443
BshFI	GGCC	8 cut(s)	187, 645, 1253, 1531, 1983, 2428, 2496, 2959
BshNI	GGYRCC	1 cut(s)	2339
BsiHKAI	GWGCWC	1 cut(s)	1861
BsiHKCI	CYCGRG	1 cut(s)	2664
BsiSI	CCGG	9 cut(s)	18, 191, 254, 436, 542, 1532, 2352, 2631, 2960
BslFI	GGGAC	5 cut(s)	436, 1303, 1375, 1480, 1488
BslI	CCNNNNNNNGG	6 cut(s)	24, 379, 1260, 1845, 2158, 3060
BsmAI	GTCTC	8 cut(s)	345, 1140, 1302, 2018, 2096, 2661, 2857, 2858
BsmFI	GGGAC	5 cut(s)	436, 1303, 1375, 1480, 1488
BsmI	GAATGC	3 cut(s)	145, 520, 2325
BsnI	GGCC	8 cut(s)	187, 645, 1253, 1531, 1983, 2428, 2496, 2959
Bso31I	GGTCTC	4 cut(s)	345, 1140, 2018, 2858
BsoBI	CYCGRG	1 cut(s)	2664
Bsp1286I	GDGCHC	1 cut(s)	1861
Bsp1407I	TGTACA	1 cut(s)	2952
Bsp19I	CCATGG	1 cut(s)	2283
BspACI	CCGC	6 cut(s)	62, 179, 188, 562, 1321, 2508
BspANI	GGCC	8 cut(s)	187, 645, 1253, 1531, 1983, 2428, 2496, 2959
BspCNI	CTCAG	5 cut(s)	735, 1363, 1962, 2028, 2092
BspLI	GGNNCC	8 cut(s)	16, 1014, 1196, 1606, 2186, 2229, 2341, 3082
BspMAI	CTGCAG	1 cut(s)	1757
BspMI	ACCTGC	2 cut(s)	573, 1057
BspT107I	GGYRCC	1 cut(s)	2339
BspTNI	GGTCTC	4 cut(s)	345, 1140, 2018, 2858
BsrDI	GCAATG	6 cut(s)	484, 866, 2895, 2899, 2970, 2982
BsrFI	RCCGGY	2 cut(s)	541, 2959
BsrGI	TGTACA	1 cut(s)	2952
BsrI	ACTGG	5 cut(s)	403, 1198, 1493, 1984, 2583
BssAI	RCCGGY	2 cut(s)	541, 2959
BssECI	CCNNGG	7 cut(s)	254, 483, 1239, 1258, 1984, 2283, 2788
BssNAI	GTATAC	1 cut(s)	1668
BssNI	GRCGYC	1 cut(s)	2349
BssT1I	CCWWGG	3 cut(s)	483, 1258, 2283
Bst1107I	GTATAC	1 cut(s)	1668
Bst2UI	CCWGG	2 cut(s)	1241, 2790
Bst6I	CTCTTC	1 cut(s)	2574
BstACI	GRCGYC	1 cut(s)	2349
BstAPI	GCANNNNNTGC	2 cut(s)	1330, 2776
BstAUI	TGTACA	1 cut(s)	2952
BstBAI	YACGTR	4 cut(s)	1578, 1698, 2773, 3060
BstDSI	CCRYGG	1 cut(s)	2283
BstH2I	RGCGCY	1 cut(s)	542
BstHHI	GCGC	2 cut(s)	541, 3005
BstMAI	GTCTC	8 cut(s)	345, 1140, 1302, 2018, 2096, 2661, 2857, 2858
BstNI	CCWGG	2 cut(s)	1241, 2790
BstNSI	RCATGY	4 cut(s)	763, 862, 1631, 2703
BstPAI	GACNNNNGTC	1 cut(s)	249
BstSCI	CCNGG	6 cut(s)	253, 435, 1239, 1531, 2629, 2788
BstSFI	CTRYAG	5 cut(s)	681, 1041, 1753, 1940, 2304
BstSNI	TACGTA	1 cut(s)	1698
BstV2I	GAAGAC	1 cut(s)	413
BstX2I	RGATCY	6 cut(s)	20, 1194, 1444, 1604, 2227, 2239
BstXI	CCANNNNNNTGG	2 cut(s)	1205, 2504
BstYI	RGATCY	6 cut(s)	20, 1194, 1444, 1604, 2227, 2239
BstZ17I	GTATAC	1 cut(s)	1668
BsuRI	GGCC	8 cut(s)	187, 645, 1253, 1531, 1983, 2428, 2496, 2959
BtgI	CCRYGG	1 cut(s)	2283
BtgZI	GCGATG	1 cut(s)	2171
BtsI	GCAGTG	2 cut(s)	204, 581
BtsIMutI	CAGTG	4 cut(s)	204, 581, 1040, 1949
BveI	ACCTGC	2 cut(s)	573, 1057
CaiI	CAGNNNCTG	3 cut(s)	1049, 1292, 2129
CfoI	GCGC	2 cut(s)	541, 3005
Cfr10I	RCCGGY	2 cut(s)	541, 2959
Cfr13I	GGNCC	5 cut(s)	185, 928, 1012, 2287, 2841
CseI	GACGC	2 cut(s)	2357, 2549
Csp6I	GTAC	8 cut(s)	392, 840, 1046, 1699, 2340, 2586, 2673, 2953
CspCI	CAANNNNNGTGG	4 cut(s)	1150, 1185, 1495, 1530
CviQI	GTAC	8 cut(s)	392, 840, 1046, 1699, 2340, 2586, 2673, 2953
DraI	TTTAAA	5 cut(s)	93, 432, 1218, 1429, 2533
DraIII	CACNNNGTG	1 cut(s)	2776
DriI	GACNNNNNGTC	2 cut(s)	506, 1492
EaeI	YGGCCR	4 cut(s)	643, 1251, 1981, 2494
Eam1104I	CTCTTC	1 cut(s)	2574
Eam1105I	GACNNNNNGTC	2 cut(s)	506, 1492
EarI	CTCTTC	1 cut(s)	2574
Eco105I	TACGTA	1 cut(s)	1698
Eco130I	CCWWGG	3 cut(s)	483, 1258, 2283
Eco147I	AGGCCT	1 cut(s)	2428
Eco31I	GGTCTC	4 cut(s)	345, 1140, 2018, 2858
Eco32I	GATATC	2 cut(s)	1761, 2785
Eco47I	GGWCC	4 cut(s)	928, 1012, 2287, 2841
Eco57I	CTGAAG	2 cut(s)	1059, 3051
Eco72I	CACGTG	3 cut(s)	1578, 2773, 3060
Eco88I	CYCGRG	1 cut(s)	2664
EcoO109I	RGGNCCY	3 cut(s)	928, 1012, 2841
EcoRI	GAATTC	1 cut(s)	1264
EcoRII	CCWGG	2 cut(s)	1239, 2788
EcoRV	GATATC	2 cut(s)	1761, 2785
EcoT14I	CCWWGG	3 cut(s)	483, 1258, 2283
EcoT22I	ATGCAT	3 cut(s)	522, 1769, 2701
ErhI	CCWWGG	3 cut(s)	483, 1258, 2283
FalI	AAGNNNNNCTT	6 cut(s)	952, 984, 1348, 1380, 2303, 2335
FaqI	GGGAC	5 cut(s)	436, 1303, 1375, 1480, 1488
FauI	CCCGC	1 cut(s)	2501
FauNDI	CATATG	1 cut(s)	1228
FbaI	TGATCA	1 cut(s)	1933
FblI	GTMKAC	2 cut(s)	318, 1667
GlaI	GCGC	2 cut(s)	540, 3004
GsuI	CTGGAG	3 cut(s)	302, 2099, 2839
HaeII	RGCGCY	1 cut(s)	542
HaeIII	GGCC	8 cut(s)	187, 645, 1253, 1531, 1983, 2428, 2496, 2959
HapII	CCGG	9 cut(s)	18, 191, 254, 436, 542, 1532, 2352, 2631, 2960
HgaI	GACGC	2 cut(s)	2357, 2549
HhaI	GCGC	2 cut(s)	541, 3005
Hin1I	GRCGYC	1 cut(s)	2349
Hin6I	GCGC	2 cut(s)	539, 3003
HinP1I	GCGC	2 cut(s)	539, 3003
HincII	GTYRAC	1 cut(s)	2347
HindII	GTYRAC	1 cut(s)	2347
HindIII	AAGCTT	3 cut(s)	353, 366, 2649
HinfI	GANTC	7 cut(s)	820, 1235, 1405, 1480, 1989, 2214, 2563
HpaII	CCGG	9 cut(s)	18, 191, 254, 436, 542, 1532, 2352, 2631, 2960
HphI	GGTGA	2 cut(s)	125, 3018
Hpy166II	GTNNAC	7 cut(s)	319, 890, 956, 1555, 1668, 2347, 2953
Hpy8I	GTNNAC	7 cut(s)	319, 890, 956, 1555, 1668, 2347, 2953
Hpy99I	CGWCG	2 cut(s)	46, 501
HpyCH4IV	ACGT	5 cut(s)	1577, 1697, 1710, 2772, 3059
HpySE526I	ACGT	5 cut(s)	1577, 1697, 1710, 2772, 3059
Hsp92I	GRCGYC	1 cut(s)	2349
HspAI	GCGC	2 cut(s)	539, 3003
KpnI	GGTACC	1 cut(s)	2343
KroI	GCCGGC	1 cut(s)	2959
KroNI	GCCGGC	1 cut(s)	2961
Ksp22I	TGATCA	1 cut(s)	1933
LmnI	GCTCC	3 cut(s)	14, 1585, 2190
LweI	GCATC	7 cut(s)	887, 914, 1061, 1072, 1308, 1776, 2602
MaeII	ACGT	5 cut(s)	1577, 1697, 1710, 2772, 3059
MaeIII	GTNAC	5 cut(s)	1520, 1706, 1975, 2198, 2219
MfeI	CAATTG	2 cut(s)	1776, 2088
MflI	RGATCY	6 cut(s)	20, 1194, 1444, 1604, 2227, 2239
MhlI	GDGCHC	1 cut(s)	1861
MlsI	TGGCCA	2 cut(s)	645, 1253
MluNI	TGGCCA	2 cut(s)	645, 1253
MlyI	GAGTC	3 cut(s)	1414, 2208, 2557
MmeI	TCCRAC	5 cut(s)	30, 65, 243, 780, 2364
Mox20I	TGGCCA	2 cut(s)	645, 1253
Mph1103I	ATGCAT	3 cut(s)	522, 1769, 2701
MroNI	GCCGGC	1 cut(s)	2959
MscI	TGGCCA	2 cut(s)	645, 1253
MslI	CAYNNNNRTG	1 cut(s)	1033
Msp20I	TGGCCA	2 cut(s)	645, 1253
MspA1I	CMGCKG	1 cut(s)	562
MspI	CCGG	9 cut(s)	18, 191, 254, 436, 542, 1532, 2352, 2631, 2960
MspR9I	CCNGG	6 cut(s)	255, 437, 1241, 1533, 2631, 2790
MunI	CAATTG	2 cut(s)	1776, 2088
Mva1269I	GAATGC	3 cut(s)	145, 520, 2325
MvaI	CCWGG	2 cut(s)	1241, 2790
NaeI	GCCGGC	1 cut(s)	2961
NciI	CCSGG	4 cut(s)	255, 437, 1533, 2631
NcoI	CCATGG	1 cut(s)	2283
NdeI	CATATG	1 cut(s)	1228
NgoMIV	GCCGGC	1 cut(s)	2959
NlaIV	GGNNCC	8 cut(s)	16, 1014, 1196, 1606, 2186, 2229, 2341, 3082
NmeAIII	GCCGAG	2 cut(s)	2009, 2071
NmuCI	GTSAC	3 cut(s)	1706, 2198, 2219
NsiI	ATGCAT	3 cut(s)	522, 1769, 2701
NspI	RCATGY	4 cut(s)	763, 862, 1631, 2703
OliI	CACNNNNGTG	1 cut(s)	1033
PaeI	GCATGC	1 cut(s)	1631
PaeR7I	CTCGAG	1 cut(s)	2664
PceI	AGGCCT	1 cut(s)	2428
PciI	ACATGT	1 cut(s)	858
PcsI	WCGNNNNNNNCGW	1 cut(s)	1414
PctI	GAATGC	3 cut(s)	145, 520, 2325
PdiI	GCCGGC	1 cut(s)	2961
PfeI	GAWTC	4 cut(s)	820, 1235, 1480, 1989
PflFI	GACNNNGTC	3 cut(s)	46, 1294, 2217
PflMI	CCANNNNNTGG	1 cut(s)	2158
PfoI	TCCNGGA	1 cut(s)	2629
PleI	GAGTC	3 cut(s)	1413, 2208, 2557
PmaCI	CACGTG	3 cut(s)	1578, 2773, 3060
PmlI	CACGTG	3 cut(s)	1578, 2773, 3060
PpsI	GAGTC	3 cut(s)	1413, 2208, 2557
Ppu21I	YACGTR	4 cut(s)	1578, 1698, 2773, 3060
PpuMI	RGGWCCY	3 cut(s)	928, 1012, 2841
PscI	ACATGT	1 cut(s)	858
PshAI	GACNNNNGTC	1 cut(s)	249
PsiI	TTATAA	1 cut(s)	2168
Psp5II	RGGWCCY	3 cut(s)	928, 1012, 2841
Psp6I	CCWGG	2 cut(s)	1239, 2788
PspCI	CACGTG	3 cut(s)	1578, 2773, 3060
PspGI	CCWGG	2 cut(s)	1239, 2788
PspN4I	GGNNCC	8 cut(s)	16, 1014, 1196, 1606, 2186, 2229, 2341, 3082
PspPI	GGNCC	5 cut(s)	185, 928, 1012, 2287, 2841
PspPPI	RGGWCCY	3 cut(s)	928, 1012, 2841
PstI	CTGCAG	1 cut(s)	1757
PstNI	CAGNNNCTG	3 cut(s)	1049, 1292, 2129
PsuI	RGATCY	6 cut(s)	20, 1194, 1444, 1604, 2227, 2239
PsyI	GACNNNGTC	3 cut(s)	46, 1294, 2217
RsaI	GTAC	8 cut(s)	393, 841, 1047, 1700, 2341, 2587, 2674, 2954
RsaNI	GTAC	8 cut(s)	392, 840, 1046, 1699, 2340, 2586, 2673, 2953
RseI	CAYNNNNRTG	1 cut(s)	1033
Sau96I	GGNCC	5 cut(s)	185, 928, 1012, 2287, 2841
ScaI	AGTACT	1 cut(s)	2587
SchI	GAGTC	3 cut(s)	1414, 2208, 2557
ScrFI	CCNGG	6 cut(s)	255, 437, 1241, 1533, 2631, 2790
SduI	GDGCHC	1 cut(s)	1861
SfaNI	GCATC	7 cut(s)	887, 914, 1061, 1072, 1308, 1776, 2602
SfcI	CTRYAG	5 cut(s)	681, 1041, 1753, 1940, 2304
Sfr274I	CTCGAG	1 cut(s)	2664
SgrAI	CRCCGGYG	1 cut(s)	541
SinI	GGWCC	4 cut(s)	928, 1012, 2287, 2841
SlaI	CTCGAG	1 cut(s)	2664
SmiI	ATTTAAAT	2 cut(s)	93, 1218
SmiMI	CAYNNNNRTG	1 cut(s)	1033
SmlI	CTYRAG	6 cut(s)	350, 882, 1141, 1269, 2333, 2664
SmoI	CTYRAG	6 cut(s)	350, 882, 1141, 1269, 2333, 2664
SnaBI	TACGTA	1 cut(s)	1698
SphI	GCATGC	1 cut(s)	1631
SseBI	AGGCCT	1 cut(s)	2428
SsiI	CCGC	6 cut(s)	62, 179, 188, 562, 1321, 2508
StuI	AGGCCT	1 cut(s)	2428
StyD4I	CCNGG	6 cut(s)	253, 435, 1239, 1531, 2629, 2788
StyI	CCWWGG	3 cut(s)	483, 1258, 2283
SwaI	ATTTAAAT	2 cut(s)	93, 1218
TaiI	ACGT	5 cut(s)	1580, 1700, 1713, 2775, 3062
TaqI	TCGA	2 cut(s)	1485, 2665
TaqII	GACCGA	3 cut(s)	1164, 2207, 2882
TatI	WGTACW	3 cut(s)	2585, 2672, 2952
TauI	GCSGC	2 cut(s)	190, 1324
TfiI	GAWTC	4 cut(s)	820, 1235, 1480, 1989
TscAI	CASTG	4 cut(s)	211, 581, 1040, 1949
TseFI	GTSAC	3 cut(s)	1706, 2198, 2219
Tsp45I	GTSAC	3 cut(s)	1706, 2198, 2219
TspGWI	ACGGA	3 cut(s)	1330, 1427, 1550
TspRI	CASTG	4 cut(s)	211, 581, 1040, 1949
Tth111I	GACNNNGTC	3 cut(s)	46, 1294, 2217
Van91I	CCANNNNNTGG	1 cut(s)	2158
VpaK11BI	GGWCC	4 cut(s)	928, 1012, 2287, 2841
XapI	RAATTY	4 cut(s)	1264, 1282, 2207, 2522
XbaI	TCTAGA	1 cut(s)	1564
XceI	RCATGY	4 cut(s)	763, 862, 1631, 2703
XhoI	CTCGAG	1 cut(s)	2664
XmaJI	CCTAGG	1 cut(s)	1258
XmiI	GTMKAC	2 cut(s)	318, 1667
ZrmI	AGTACT	1 cut(s)	2587
Zsp2I	ATGCAT	3 cut(s)	522, 1769, 2701

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.