Rorug04G0216800

Squamosa promoter-binding-like protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000004
OG0001572
Physical Location & Seq
Forward (+)
37488883 .. 37490289
1407 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug04G0216800.1

Sequence Viewer

Length: 369 bp
ATGGAGATTGAGATGGTCAAGTGCGAGTGTTGTGGACTAAAAGAGGATTGCACGCAAGACTACATCACTCAAGTGAAAGCGAAATTCGACGGCAAATGGCTCTGTGGGTTGTGCTCGGAAGCAGTAAGAGACGAGGTTAGCAAAGGCAAGAAGCCATTCGGCATGGAAGAAGCTGTGAGAGCTCACATGTCATTTTGTGCAAAATTCAAATCCAACCCTGCAGTTCGAGTTGCAGATGGGATGAGGCAGATGCTTAGAAGAAGGTCAGACATGTCCTCATCATCGTCATCTTCGTCTTCATCGAAGAAGTATACAAGATCATCAAGCACATCCCAAGTGGGTGATTCGTCTTCTTTCTCATTGTACTAA

Protein Analysis

122

Amino Acids

13.54

Weight (kDa)

8.87

Isoelectric Point (pI)

73.38

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF1677 PF07911 3 - 90 1.3e-38 Protein of unknown function (DUF1677)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccI GTMKAC 1 cut(s) 311
AcsI RAATTY 2 cut(s) 83, 203
AfaI GTAC 1 cut(s) 365
AflIII ACRYGT 2 cut(s) 186, 270
AgsI TTSAA 1 cut(s) 208
AleI CACNNNNGTG 1 cut(s) 71
AluBI AGCT 2 cut(s) 173, 182
AluI AGCT 2 cut(s) 173, 182
Alw21I GWGCWC 2 cut(s) 116, 184
Alw26I GTCTC 1 cut(s) 123
ApoI RAATTY 2 cut(s) 83, 203
Asp700I GAANNNNTTC 1 cut(s) 155
AsuHPI GGTGA 1 cut(s) 353
BanII GRGCYC 1 cut(s) 184
BbsI GAAGAC 2 cut(s) 288, 342
Bbv12I GWGCWC 2 cut(s) 116, 184
BccI CCATC 2 cut(s) 7, 230
BceAI ACGGC 1 cut(s) 106
BcoDI GTCTC 1 cut(s) 123
BfmI CTRYAG 1 cut(s) 219
BmsI GCATC 1 cut(s) 240
BpiI GAAGAC 2 cut(s) 288, 342
BpuEI CTTGAG 1 cut(s) 54
BseGI GGATG 2 cut(s) 246, 329
BsiHKAI GWGCWC 2 cut(s) 116, 184
BsmAI GTCTC 1 cut(s) 123
BsmBI CGTCTC 1 cut(s) 123
Bsp1286I GDGCHC 2 cut(s) 116, 184
Bsp143I GATC 1 cut(s) 317
BspMAI CTGCAG 1 cut(s) 223
BssMI GATC 1 cut(s) 317
BssNAI GTATAC 1 cut(s) 312
Bst1107I GTATAC 1 cut(s) 312
BstC8I GCNNGC 1 cut(s) 53
BstDEI CTNAG 1 cut(s) 254
BstF5I GGATG 2 cut(s) 246, 329
BstKTI GATC 1 cut(s) 320
BstMAI GTCTC 1 cut(s) 123
BstMBI GATC 1 cut(s) 317
BstMWI GCNNNNNNNGC 1 cut(s) 179
BstNSI RCATGY 2 cut(s) 190, 274
BstSFI CTRYAG 1 cut(s) 219
BstV2I GAAGAC 2 cut(s) 288, 342
BstZ17I GTATAC 1 cut(s) 312
BtsCI GGATG 2 cut(s) 246, 329
Cac8I GCNNGC 1 cut(s) 53
Csp6I GTAC 1 cut(s) 364
CviAII CATG 3 cut(s) 163, 187, 271
CviJI RGCY 4 cut(s) 100, 154, 173, 182
CviKI_1 RGCY 4 cut(s) 100, 154, 173, 182
CviQI GTAC 1 cut(s) 364
DdeI CTNAG 1 cut(s) 254
DpnI GATC 1 cut(s) 319
DpnII GATC 1 cut(s) 317
Ecl136II GAGCTC 1 cut(s) 182
Eco24I GRGCYC 1 cut(s) 184
Eco53kI GAGCTC 1 cut(s) 182
EcoICRI GAGCTC 1 cut(s) 182
EcoT38I GRGCYC 1 cut(s) 184
Esp3I CGTCTC 1 cut(s) 123
FaeI CATG 3 cut(s) 166, 190, 274
FaiI YATR 4 cut(s) 164, 188, 272, 312
FatI CATG 3 cut(s) 162, 186, 270
FblI GTMKAC 1 cut(s) 311
FokI GGATG 2 cut(s) 253, 316
FriOI GRGCYC 1 cut(s) 184
Hin1II CATG 3 cut(s) 166, 190, 274
HinfI GANTC 1 cut(s) 344
HphI GGTGA 1 cut(s) 353
Hpy166II GTNNAC 2 cut(s) 35, 312
Hpy188I TCNGA 2 cut(s) 118, 268
Hpy8I GTNNAC 2 cut(s) 35, 312
Hpy99I CGWCG 1 cut(s) 92
HpyAV CCTTC 1 cut(s) 255
HpyCH4V TGCA 4 cut(s) 51, 200, 221, 233
HpyF10VI GCNNNNNNNGC 1 cut(s) 179
HpyF3I CTNAG 1 cut(s) 254
Hsp92II CATG 3 cut(s) 166, 190, 274
Kzo9I GATC 1 cut(s) 317
LpnPI CCDG 1 cut(s) 231
LweI GCATC 1 cut(s) 240
MalI GATC 1 cut(s) 319
MboI GATC 1 cut(s) 317
MboII GAAGA 6 cut(s) 179, 270, 282, 288, 316, 342
MhlI GDGCHC 2 cut(s) 116, 184
MluCI AATT 2 cut(s) 83, 203
MmeI TCCRAC 1 cut(s) 237
MnlI CCTC 4 cut(s) 37, 127, 237, 286
MroXI GAANNNNTTC 1 cut(s) 155
MslI CAYNNNNRTG 1 cut(s) 71
MwoI GCNNNNNNNGC 1 cut(s) 179
NdeII GATC 1 cut(s) 317
NlaIII CATG 3 cut(s) 166, 190, 274
NspI RCATGY 2 cut(s) 190, 274
OliI CACNNNNGTG 1 cut(s) 71
PciI ACATGT 2 cut(s) 186, 270
PcsI WCGNNNNNNNCGW 2 cut(s) 290, 299
PdmI GAANNNNTTC 1 cut(s) 155
PfeI GAWTC 1 cut(s) 344
PscI ACATGT 2 cut(s) 186, 270
Psp124BI GAGCTC 1 cut(s) 184
PstI CTGCAG 1 cut(s) 223
RsaI GTAC 1 cut(s) 365
RsaNI GTAC 1 cut(s) 364
RseI CAYNNNNRTG 1 cut(s) 71
SacI GAGCTC 1 cut(s) 184
Sau3AI GATC 1 cut(s) 317
SduI GDGCHC 2 cut(s) 116, 184
SetI ASST 4 cut(s) 138, 175, 184, 266
SfaNI GCATC 1 cut(s) 240
SfcI CTRYAG 1 cut(s) 219
SmiMI CAYNNNNRTG 1 cut(s) 71
SmlI CTYRAG 1 cut(s) 69
SmoI CTYRAG 1 cut(s) 69
Sse9I AATT 2 cut(s) 83, 203
SstI GAGCTC 1 cut(s) 184
TaqI TCGA 3 cut(s) 87, 226, 302
TasI AATT 2 cut(s) 83, 203
TatI WGTACW 1 cut(s) 363
TfiI GAWTC 1 cut(s) 344
TspDTI ATGAA 1 cut(s) 288
XapI RAATTY 2 cut(s) 83, 203
XceI RCATGY 2 cut(s) 190, 274
XmiI GTMKAC 1 cut(s) 311
XmnI GAANNNNTTC 1 cut(s) 155
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.