Basic Information

Type: gene

Biological Identity

Species

rosa_samantha

Chromosome

Chr4C

Orthogroup ID

OG0001572

Physical Location & Seq

Genomic Coordinates Reverse (-)

55334831 .. 55343022

Gene Structure 8192 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

Rh4CG294100.1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2340 bp

ATGATGTCATTACCCTTTCCTGAGGCTGTTCTTTTTTATCATTTTTTCTTTTCAGCTAATAGTTCTGATCAAACGAAGGATCAGGATCTGCTATCTCACCTGTTGAAGAGCGTAGCCAATCTTGCTGGTACAGTTGATGGGAGAAACATATCTGCATTACTTCAGGCATCTCAAGGTTTACCTAACACTGGGTCTTCCATCAAGACTGCACAACAGGTTCCAGATACGGTTTCTAACATCTTTGAACCCAGTAGGCCTTCTGTTTCTGCCTCAAGTATGGATGATTGTGTTATTATTGAGGAAACTTTAAGGCCCATAGGACAGTGCCCGATATTACCTGCATCAGATATGCAAAAGAGAAGATCTTCAGTTGATGGCGATCTTGGAAGTCAAATTACTTCAGGTCTACAGGCCAACAAGCCACTTCCTTCAAGAGAAAGTGCTCCAACCAATTCAGTTACACCGATCCAGTTACTTGAGATTGATTTAAATAGTCCATATGATGATTCACAGGACTATTTAGAGAACCTAGGGACTTGTCATGTCACTATAAATCCAGGGATACAACAAGATTCTCACAAGTCAAGCCCACCTCAGACCAGTGGGAATTCAGACTCAACTTTTACTCAGTCACCATCAAGTTCTAGTGGAGAATCTCAGAATCGCACAGACCGCATTGTTTTTAAACTCTTTGGAAAAGACCCGAATGAACTTCCATTTGTTCTGCGATCACAGATTATCGACTGGTTATCCCATAGTCCTACAGAGATTGAAAGCTATATAAGGCCAGGATGCATCATTTTGACAATTTACCTCCGTCTAGAAAAATCCACGTGGGAGGAGCTCTGCTGTCATCTGGGATCCAGTTTGCAAAGACTTTTAGATGCCGCCAATGATCCTTTCTGGAGAACAGGATGGGTGTATACTAGGATGCAACATTTTGTTGCGTTTATGTACAATGGTCAAGTTGTCTTAGACACAACCTTACCTCTTAAAAGTCAAAAAAGTTGCAGGATATCAAGCATTAAACCAATTGCAGTCTCTTTATCTGAGAGAGCCCAGTTTGTTGTAAAAGGCTTTAACCTTCCTCATTCCACCACAAGGTTACTATGTGCTCTAGAAGGGAAGTATCTGGCGCAAGAAGCTTGTGATGACGTGATGGATGGTGCTGATACAACTGTTGAGCATGATGAGCTACAGTGCATCAGATTCTCCTGCTCTATACCAAATGTTACTGGGCGAGGATTCATTGAGGTTGAAGATCTTGGTCTCAGTAGCAGCTTCTTTCCATTTATAGTTGCAGAGCAGGAAGTGTGTTCAGAGATTTGTATGTTGGAGGATGCGATAGAGGCAGCTGAGACTGTTGATGACATCCAGGCAGATCCTGTAATATTAGAAGCCAAGAACCAGGCTATGGACTTTATACATGAATTGGGTTGGCTTCTTCATAGAAGTCATGTTAAGTTTAGACTTGGTCACCTGGATCCCAACCTAGATCTATTTCCTTTTAGACGGTTCAAATTACTTATGGAGTTTTCCGTGGATCATGACTGGTGTGCTGTAGTGAAGAAACTCTTGAAGCTTCTGTTTGACAGTACTGTTGATGCCGGAGAACATCCTTCCGTTCAGCTTGCATTATTGGATATGGCCCTCCTCCACAGAGCTGTTCGAAGAAATTGCAGACCTATGGTAGAACTGTTGTTAAGATTTGTTCCAGATAAAGGTTTAGAATCTGAACAGAAGAAATACGTTGAAGGGGAAGACAACAGTTTCTTATTTAAACCTGATGGTGTTGGTCCCCTGGGGTTGACTCCTCTTCATGTTGCAGCTAGTATTGATGGTTGTGAGCACGTATTGGATGCTTTAACTGATGATCCTGGGAAGGTGGGAATTGAAGCTTGGAAAAATGCTCGAGACAGTACAGGAATGACACCATATGATTATGCATCCATGCAAGGCCGCTACTCCTATATCAATCTTGTTCAGAGGAAAATCAGCAAGAAACTTGAGAGTGGGCATGTGGTTCTTGATATCCCCGGCACTGTGTTAGAGAGCAATAGCAAGCAGAAGCAATCAGATGGGCATAGATCATCAAAAGTAGCTAGCCTTGACACCGAAAAGTTTGAAATAAATGCATTAATACGAAGAGATTGCAAGTTATGTAGCCAGAAACTAGCTTATGGCAGCACAAGGTCACTGGTGTACAGGCCGGCAATGTTGTCAATGGTGGCCATTGCTGCCGTCTGTGTCTGTGTGGCCTTGCTCTTTAAGAGCACCCCTGAAGTTGTTTTCATCTTCCACCCCTTCCGGTGGGAGCATTTGAAGTTTGGCTCAAGCTAA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003700 GO:0006355 GO:0008150 GO:0009889 GO:0010468 GO:0010556 GO:0019219 GO:0019222 GO:0031323 GO:0031326 GO:0050789 GO:0050794 GO:0051171 GO:0051252 GO:0060255 GO:0065007 GO:0080090 GO:0140110 GO:1903506 GO:2000112 GO:2001141

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

SBP

Protein Analysis

779

Amino Acids

86.56

Weight (kDa)

5.45

Isoelectric Point (pI)

47.28

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Ig_SPL7	PF26102	333 - 444	1.1e-44	SPL7-like, immunoglobulin-like domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0001572)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT2G47070 AT2G47070 AT3G60030
fragaria_vesca	FvH4_4g21420 FvH4_4g21420 FvH4_4g21420 FvH4_4g21420 FvH4_4g21420 FvH4_4g21420 FvH4_4g21420 FvH4_4g21420 FvH4_4g21420 FvH4_4g21420 FvH4_4g21420 FvH4_4g21420 FvH4_4g21420 FvH4_4g21420 FvH4_4g21420 FvH4_4g21420
malus_domestica	MD13G1141300.v1.1 MD13G1141400.v1.1 MD16G1137600.v1.1 MD16G1137700.v1.1
prunus_persica	Prupe.1G106500_v2.0.a1 Prupe.1G106500_v2.0.a1 Prupe.1G106600_v2.0.a1
pyrus_communis	pycom13g12280 pycom13g12290 pycom16g12140 pycom16g12150 pycom16g12160
rosa_chinensis	RchiOBHm_Chr4g0427341 RchiOBHm_Chr4g0427351
rosa_laevigata	RLG00000007250 RLG00000007251
rosa_multiflora	Rmu_sc0003813.1_g000017 Rmu_sc0003813.1_g000018
rosa_roxburghii	Rroxscaffold_5G00369020 Rroxscaffold_5G00369030
rosa_rugosa	Rorug04G0216800
rosa_samantha	Rh4AG273100 Rh4AG273300 Rh4BG278800 Rh4BG278900 Rh4CG294100 Rh4CG294200 Rh4DG276200 Rh4DG276300
rosa_wichuraiana	Rw4G023700 Rw4G023710

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
Acc36I	ACCTGC	1 cut(s)	346
AccB7I	CCANNNNNTGG	1 cut(s)	1414
AccI	GTMKAC	2 cut(s)	406, 923
AciI	CCGC	3 cut(s)	673, 888, 1960
AcoI	YGGCCR	1 cut(s)	2229
AcsI	RAATTY	1 cut(s)	607
AcuI	CTGAAG	4 cut(s)	146, 351, 384, 2301
AcvI	CACGTG	1 cut(s)	834
AfaI	GTAC	5 cut(s)	130, 956, 1597, 1921, 2204
AfiI	CCNNNNNNNGG	5 cut(s)	188, 1101, 1414, 1721, 2310
AjiI	CACGTC	1 cut(s)	1156
AjnI	CCWGG	7 cut(s)	556, 787, 1374, 1407, 1479, 1800, 1876
AjuI	GAANNNNNNNTTGG	4 cut(s)	884, 916, 1882, 1914
Alw21I	GWGCWC	5 cut(s)	445, 846, 1117, 1851, 2276
Alw26I	GTCTC	4 cut(s)	1045, 1274, 1352, 1908
AlwNI	CAGNNNCTG	2 cut(s)	88, 1385
Ama87I	CYCGRG	1 cut(s)	1911
AoxI	GGCC	9 cut(s)	254, 311, 411, 785, 1647, 1957, 2207, 2229, 2256
ApeKI	GCWGC	5 cut(s)	1278, 1352, 1826, 2184, 2237
ApoI	RAATTY	1 cut(s)	607
AseI	ATTAAT	1 cut(s)	2138
Asp700I	GAANNNNTTC	1 cut(s)	364
AspA2I	CCTAGG	1 cut(s)	529
AspLEI	GCGC	1 cut(s)	1138
AspS9I	GGNCC	3 cut(s)	312, 1648, 1796
AsuC2I	CCSGG	1 cut(s)	2037
AsuHPI	GGTGA	3 cut(s)	89, 624, 1469
AsuII	TTCGAA	1 cut(s)	1669
AsuNHI	GCTAGC	1 cut(s)	2102
AvaI	CYCGRG	1 cut(s)	1911
AvaII	GGWCC	1 cut(s)	1796
AvrII	CCTAGG	1 cut(s)	529
AxyI	CCTNAGG	1 cut(s)	21
BaeGI	GKGCMC	1 cut(s)	329
BaeI	ACNNNNGTAYC	2 cut(s)	1164, 1197
BalI	TGGCCA	1 cut(s)	2231
BamHI	GGATCC	2 cut(s)	860, 1483
BanII	GRGCYC	2 cut(s)	846, 1060
BarI	GAAGNNNNNNTAC	2 cut(s)	379, 411
BbrPI	CACGTG	1 cut(s)	834
BbsI	GAAGAC	2 cut(s)	186, 1767
Bbv12I	GWGCWC	5 cut(s)	445, 846, 1117, 1851, 2276
BbvI	GCAGC	5 cut(s)	1290, 1364, 1838, 2196, 2224
BceAI	ACGGC	1 cut(s)	2225
BcgI	CGANNNNNNTGC	4 cut(s)	1659, 1693, 2133, 2167
BciT130I	CCWGG	7 cut(s)	558, 789, 1376, 1409, 1481, 1802, 1878
BciVI	GTATCC	1 cut(s)	555
BclI	TGATCA	1 cut(s)	67
BcnI	CCSGG	1 cut(s)	2037
BcoDI	GTCTC	4 cut(s)	1045, 1274, 1352, 1908
BfaI	CTAG	9 cut(s)	530, 645, 821, 927, 1118, 1493, 1830, 2103, 2174
BfmI	CTRYAG	4 cut(s)	407, 762, 1196, 1560
BfuAI	ACCTGC	1 cut(s)	346
BfuI	GTATCC	1 cut(s)	555
BglII	AGATCT	3 cut(s)	362, 1261, 1495
BisI	GCNGC	7 cut(s)	888, 1279, 1353, 1827, 1960, 2185, 2238
BlnI	CCTAGG	1 cut(s)	529
BlsI	GCNGC	7 cut(s)	889, 1280, 1354, 1828, 1961, 2186, 2239
BmcAI	AGTACT	1 cut(s)	1597
Bme1390I	CCNGG	8 cut(s)	558, 789, 1376, 1409, 1481, 1802, 1878, 2037
Bme18I	GGWCC	1 cut(s)	1796
BmeT110I	CYCGRG	1 cut(s)	1911
BmgBI	CACGTC	1 cut(s)	1156
BmgT120I	GGNCC	3 cut(s)	312, 1648, 1796
BmiI	GGNNCC	4 cut(s)	219, 862, 1485, 1798
BmrFI	CCNGG	8 cut(s)	558, 789, 1376, 1409, 1481, 1802, 1878, 2037
BmrI	ACTGGG	4 cut(s)	198, 243, 1054, 1245
BmtI	GCTAGC	1 cut(s)	2106
BmuI	ACTGGG	4 cut(s)	198, 243, 1054, 1245
BpiI	GAAGAC	2 cut(s)	186, 1767
BpmI	CTGGAG	1 cut(s)	925
Bpu14I	TTCGAA	1 cut(s)	1669
BpuEI	CTTGAG	5 cut(s)	156, 256, 497, 2027, 2317
BpuMI	CCSGG	1 cut(s)	2037
BsaAI	YACGTR	2 cut(s)	834, 1852
BsaBI	GATNNNNATC	2 cut(s)	84, 378
BsaI	GGTCTC	1 cut(s)	1274
BsaJI	CCNNGG	7 cut(s)	529, 557, 1539, 1800, 1801, 1877, 2035
BsaWI	WCCGGW	1 cut(s)	2307
Bsc4I	CCNNNNNNNGG	5 cut(s)	188, 1101, 1414, 1721, 2310
Bse118I	RCCGGY	1 cut(s)	2209
Bse21I	CCTNAGG	1 cut(s)	21
Bse3DI	GCAATG	2 cut(s)	2220, 2232
Bse8I	GATNNNNATC	2 cut(s)	84, 378
BseBI	CCWGG	7 cut(s)	558, 789, 1376, 1409, 1481, 1802, 1878
BseDI	CCNNGG	7 cut(s)	529, 557, 1539, 1800, 1801, 1877, 2035
BseJI	GATNNNNATC	2 cut(s)	84, 378
BseLI	CCNNNNNNNGG	5 cut(s)	188, 1101, 1414, 1721, 2310
BseMI	GCAATG	2 cut(s)	2220, 2232
BseMII	CTCAG	7 cut(s)	12, 608, 641, 671, 1041, 1285, 1347
BseRI	GAGGAG	3 cut(s)	854, 1643, 1803
BseSI	GKGCMC	1 cut(s)	329
BseXI	GCAGC	5 cut(s)	1290, 1364, 1838, 2196, 2224
BsgI	GTGCAG	1 cut(s)	192
BshFI	GGCC	9 cut(s)	256, 313, 413, 787, 1649, 1959, 2209, 2231, 2258
BsiHKAI	GWGCWC	5 cut(s)	445, 846, 1117, 1851, 2276
BsiHKCI	CYCGRG	1 cut(s)	1911
BsiSI	CCGG	4 cut(s)	1608, 2037, 2210, 2308
BslFI	GGGAC	2 cut(s)	547, 1782
BslI	CCNNNNNNNGG	5 cut(s)	188, 1101, 1414, 1721, 2310
BsmAI	GTCTC	4 cut(s)	1045, 1274, 1352, 1908
BsmFI	GGGAC	2 cut(s)	547, 1782
BsnI	GGCC	9 cut(s)	256, 313, 413, 787, 1649, 1959, 2209, 2231, 2258
Bso31I	GGTCTC	1 cut(s)	1274
BsoBI	CYCGRG	1 cut(s)	1911
Bsp119I	TTCGAA	1 cut(s)	1669
Bsp1286I	GDGCHC	7 cut(s)	329, 445, 846, 1060, 1117, 1851, 2276
Bsp1407I	TGTACA	2 cut(s)	954, 2202
BspACI	CCGC	3 cut(s)	673, 888, 1960
BspANI	GGCC	9 cut(s)	256, 313, 413, 787, 1649, 1959, 2209, 2231, 2258
BspCNI	CTCAG	7 cut(s)	13, 607, 640, 670, 1042, 1284, 1348
BspHI	TCATGA	1 cut(s)	1546
BspLI	GGNNCC	4 cut(s)	219, 862, 1485, 1798
BspMI	ACCTGC	1 cut(s)	346
BspOI	GCTAGC	1 cut(s)	2106
BspQI	GCTCTTC	1 cut(s)	101
BspT104I	TTCGAA	1 cut(s)	1669
BspTNI	GGTCTC	1 cut(s)	1274
BsrDI	GCAATG	2 cut(s)	2220, 2232
BsrFI	RCCGGY	1 cut(s)	2209
BsrGI	TGTACA	2 cut(s)	954, 2202
BssAI	RCCGGY	1 cut(s)	2209
BssECI	CCNNGG	7 cut(s)	529, 557, 1539, 1800, 1801, 1877, 2035
BssNAI	GTATAC	1 cut(s)	924
BssT1I	CCWWGG	1 cut(s)	529
Bst1107I	GTATAC	1 cut(s)	924
Bst2UI	CCWGG	7 cut(s)	558, 789, 1376, 1409, 1481, 1802, 1878
Bst6I	CTCTTC	3 cut(s)	101, 1821, 2140
BstAUI	TGTACA	2 cut(s)	954, 2202
BstBAI	YACGTR	2 cut(s)	834, 1852
BstBI	TTCGAA	1 cut(s)	1669
BstC8I	GCNNGC	4 cut(s)	1632, 2063, 2104, 2211
BstDEI	CTNAG	8 cut(s)	21, 594, 627, 657, 973, 1050, 1271, 1356
BstDSI	CCRYGG	1 cut(s)	1539
BstEII	GGTNACC	1 cut(s)	1475
BstHHI	GCGC	1 cut(s)	1138
BstMAI	GTCTC	4 cut(s)	1045, 1274, 1352, 1908
BstMWI	GCNNNNNNNGC	6 cut(s)	122, 672, 1142, 1192, 1349, 2237
BstNI	CCWGG	7 cut(s)	558, 789, 1376, 1409, 1481, 1802, 1878
BstNSI	RCATGY	1 cut(s)	2021
BstPI	GGTNACC	1 cut(s)	1475
BstSCI	CCNGG	8 cut(s)	556, 787, 1374, 1407, 1479, 1800, 1876, 2035
BstSFI	CTRYAG	4 cut(s)	407, 762, 1196, 1560
BstSLI	GKGCMC	1 cut(s)	329
BstV1I	GCAGC	5 cut(s)	1290, 1364, 1838, 2196, 2224
BstV2I	GAAGAC	2 cut(s)	186, 1767
BstX2I	RGATCY	7 cut(s)	85, 362, 860, 1261, 1381, 1483, 1495
BstYI	RGATCY	7 cut(s)	85, 362, 860, 1261, 1381, 1483, 1495
BstZ17I	GTATAC	1 cut(s)	924
Bsu36I	CCTNAGG	1 cut(s)	21
BsuI	GTATCC	1 cut(s)	555
BsuRI	GGCC	9 cut(s)	256, 313, 413, 787, 1649, 1959, 2209, 2231, 2258
BtgI	CCRYGG	1 cut(s)	1539
BtrI	CACGTC	1 cut(s)	1156
BtsIMutI	CAGTG	6 cut(s)	186, 329, 607, 1205, 2040, 2195
BveI	ACCTGC	1 cut(s)	346
Cac8I	GCNNGC	4 cut(s)	1632, 2063, 2104, 2211
CaiI	CAGNNNCTG	2 cut(s)	88, 1385
CciI	TCATGA	1 cut(s)	1546
CfoI	GCGC	1 cut(s)	1138
Cfr10I	RCCGGY	1 cut(s)	2209
Cfr13I	GGNCC	3 cut(s)	312, 1648, 1796
Csp6I	GTAC	5 cut(s)	129, 955, 1596, 1920, 2203
CviAII	CATG	8 cut(s)	542, 1187, 1427, 1457, 1547, 1820, 1951, 2018
CviQI	GTAC	5 cut(s)	129, 955, 1596, 1920, 2203
DdeI	CTNAG	8 cut(s)	21, 594, 627, 657, 973, 1050, 1271, 1356
DraI	TTTAAA	3 cut(s)	489, 685, 1780
EaeI	YGGCCR	1 cut(s)	2229
Eam1104I	CTCTTC	3 cut(s)	101, 1821, 2140
EarI	CTCTTC	3 cut(s)	101, 1821, 2140
Ecl136II	GAGCTC	1 cut(s)	844
Eco130I	CCWWGG	1 cut(s)	529
Eco147I	AGGCCT	1 cut(s)	256
Eco24I	GRGCYC	2 cut(s)	846, 1060
Eco31I	GGTCTC	1 cut(s)	1274
Eco32I	GATATC	2 cut(s)	1017, 2032
Eco47I	GGWCC	1 cut(s)	1796
Eco53kI	GAGCTC	1 cut(s)	844
Eco57I	CTGAAG	4 cut(s)	146, 351, 384, 2301
Eco72I	CACGTG	1 cut(s)	834
Eco81I	CCTNAGG	1 cut(s)	21
Eco88I	CYCGRG	1 cut(s)	1911
Eco91I	GGTNACC	1 cut(s)	1475
EcoICRI	GAGCTC	1 cut(s)	844
EcoO65I	GGTNACC	1 cut(s)	1475
EcoRI	GAATTC	1 cut(s)	607
EcoRII	CCWGG	7 cut(s)	556, 787, 1374, 1407, 1479, 1800, 1876
EcoRV	GATATC	2 cut(s)	1017, 2032
EcoT14I	CCWWGG	1 cut(s)	529
EcoT22I	ATGCAT	3 cut(s)	797, 1948, 2137
EcoT38I	GRGCYC	2 cut(s)	846, 1060
ErhI	CCWWGG	1 cut(s)	529
FaeI	CATG	8 cut(s)	545, 1190, 1430, 1460, 1550, 1823, 1954, 2021
FalI	AAGNNNNNCTT	2 cut(s)	1559, 1591
FaqI	GGGAC	2 cut(s)	547, 1782
FatI	CATG	8 cut(s)	541, 1186, 1426, 1456, 1546, 1819, 1950, 2017
FauNDI	CATATG	2 cut(s)	499, 1936
FbaI	TGATCA	1 cut(s)	67
FblI	GTMKAC	2 cut(s)	406, 923
Fnu4HI	GCNGC	7 cut(s)	888, 1279, 1353, 1827, 1960, 2185, 2238
FriOI	GRGCYC	2 cut(s)	846, 1060
Fsp4HI	GCNGC	7 cut(s)	888, 1279, 1353, 1827, 1960, 2185, 2238
FspBI	CTAG	9 cut(s)	530, 645, 821, 927, 1118, 1493, 1830, 2103, 2174
GlaI	GCGC	1 cut(s)	1137
GluI	GCNGC	7 cut(s)	888, 1279, 1353, 1827, 1960, 2185, 2238
GsuI	CTGGAG	1 cut(s)	925
HaeIII	GGCC	9 cut(s)	256, 313, 413, 787, 1649, 1959, 2209, 2231, 2258
HapII	CCGG	4 cut(s)	1608, 2037, 2210, 2308
HhaI	GCGC	1 cut(s)	1138
Hin1II	CATG	8 cut(s)	545, 1190, 1430, 1460, 1550, 1823, 1954, 2021
Hin6I	GCGC	1 cut(s)	1136
HinP1I	GCGC	1 cut(s)	1136
HincII	GTYRAC	1 cut(s)	1809
HindII	GTYRAC	1 cut(s)	1809
HindIII	AAGCTT	3 cut(s)	1143, 1580, 1896
HinfI	GANTC	9 cut(s)	506, 572, 614, 653, 661, 1209, 1245, 1730, 1810
HpaII	CCGG	4 cut(s)	1608, 2037, 2210, 2308
HphI	GGTGA	3 cut(s)	89, 624, 1469
Hpy166II	GTNNAC	5 cut(s)	179, 407, 924, 1809, 2203
Hpy8I	GTNNAC	5 cut(s)	179, 407, 924, 1809, 2203
HpyAV	CCTTC	9 cut(s)	70, 267, 438, 1094, 1115, 1629, 1748, 1876, 2314
HpyCH4IV	ACGT	4 cut(s)	833, 1155, 1749, 1851
HpyF10VI	GCNNNNNNNGC	6 cut(s)	122, 672, 1142, 1192, 1349, 2237
HpyF3I	CTNAG	8 cut(s)	21, 594, 627, 657, 973, 1050, 1271, 1356
HpySE526I	ACGT	4 cut(s)	833, 1155, 1749, 1851
Hsp92II	CATG	8 cut(s)	545, 1190, 1430, 1460, 1550, 1823, 1954, 2021
HspAI	GCGC	1 cut(s)	1136
KroI	GCCGGC	1 cut(s)	2209
KroNI	GCCGGC	1 cut(s)	2211
Ksp22I	TGATCA	1 cut(s)	67
LguI	GCTCTTC	1 cut(s)	101
LmnI	GCTCC	3 cut(s)	448, 841, 2314
Lsp1109I	GCAGC	5 cut(s)	1290, 1364, 1838, 2196, 2224
MaeI	CTAG	9 cut(s)	530, 645, 821, 927, 1118, 1493, 1830, 2103, 2174
MaeII	ACGT	4 cut(s)	833, 1155, 1749, 1851
MaeIII	GTNAC	8 cut(s)	457, 471, 544, 630, 1104, 1231, 1475, 2193
MfeI	CAATTG	1 cut(s)	1032
MflI	RGATCY	7 cut(s)	85, 362, 860, 1261, 1381, 1483, 1495
MhlI	GDGCHC	7 cut(s)	329, 445, 846, 1060, 1117, 1851, 2276
MlsI	TGGCCA	1 cut(s)	2231
MluCI	AATT	9 cut(s)	393, 451, 607, 807, 1032, 1430, 1520, 1675, 1890
MluNI	TGGCCA	1 cut(s)	2231
MlyI	GAGTC	2 cut(s)	608, 1804
MmeI	TCCRAC	2 cut(s)	470, 1314
Mox20I	TGGCCA	1 cut(s)	2231
Mph1103I	ATGCAT	3 cut(s)	797, 1948, 2137
MroNI	GCCGGC	1 cut(s)	2209
MroXI	GAANNNNTTC	1 cut(s)	364
MscI	TGGCCA	1 cut(s)	2231
Msp20I	TGGCCA	1 cut(s)	2231
MspA1I	CMGCKG	1 cut(s)	1355
MspI	CCGG	4 cut(s)	1608, 2037, 2210, 2308
MspR9I	CCNGG	8 cut(s)	558, 789, 1376, 1409, 1481, 1802, 1878, 2037
MunI	CAATTG	1 cut(s)	1032
MvaI	CCWGG	7 cut(s)	558, 789, 1376, 1409, 1481, 1802, 1878
MwoI	GCNNNNNNNGC	6 cut(s)	122, 672, 1142, 1192, 1349, 2237
NaeI	GCCGGC	1 cut(s)	2211
NciI	CCSGG	1 cut(s)	2037
NdeI	CATATG	2 cut(s)	499, 1936
NgoMIV	GCCGGC	1 cut(s)	2209
NheI	GCTAGC	1 cut(s)	2102
NlaIII	CATG	8 cut(s)	545, 1190, 1430, 1460, 1550, 1823, 1954, 2021
NlaIV	GGNNCC	4 cut(s)	219, 862, 1485, 1798
NmuCI	GTSAC	4 cut(s)	544, 630, 1475, 2193
NsiI	ATGCAT	3 cut(s)	797, 1948, 2137
NspI	RCATGY	1 cut(s)	2021
NspV	TTCGAA	1 cut(s)	1669
PaeR7I	CTCGAG	1 cut(s)	1911
PagI	TCATGA	1 cut(s)	1546
PasI	CCCWGGG	1 cut(s)	1801
PceI	AGGCCT	1 cut(s)	256
PciSI	GCTCTTC	1 cut(s)	101
PdiI	GCCGGC	1 cut(s)	2211
PdmI	GAANNNNTTC	1 cut(s)	364
PfeI	GAWTC	7 cut(s)	506, 572, 653, 661, 1209, 1245, 1730
PflFI	GACNNNGTC	1 cut(s)	1473
PflMI	CCANNNNNTGG	1 cut(s)	1414
PkrI	GCNGC	7 cut(s)	889, 1280, 1354, 1828, 1961, 2186, 2239
PleI	GAGTC	2 cut(s)	608, 1804
PmaCI	CACGTG	1 cut(s)	834
PmlI	CACGTG	1 cut(s)	834
PpsI	GAGTC	2 cut(s)	608, 1804
Ppu21I	YACGTR	2 cut(s)	834, 1852
PshBI	ATTAAT	1 cut(s)	2138
Psp124BI	GAGCTC	1 cut(s)	846
Psp6I	CCWGG	7 cut(s)	556, 787, 1374, 1407, 1479, 1800, 1876
PspCI	CACGTG	1 cut(s)	834
PspEI	GGTNACC	1 cut(s)	1475
PspGI	CCWGG	7 cut(s)	556, 787, 1374, 1407, 1479, 1800, 1876
PspN4I	GGNNCC	4 cut(s)	219, 862, 1485, 1798
PspPI	GGNCC	3 cut(s)	312, 1648, 1796
PstNI	CAGNNNCTG	2 cut(s)	88, 1385
PsuI	RGATCY	7 cut(s)	85, 362, 860, 1261, 1381, 1483, 1495
PsyI	GACNNNGTC	1 cut(s)	1473
PvuII	CAGCTG	1 cut(s)	1355
RsaI	GTAC	5 cut(s)	130, 956, 1597, 1921, 2204
RsaNI	GTAC	5 cut(s)	129, 955, 1596, 1920, 2203
SacI	GAGCTC	1 cut(s)	846
SapI	GCTCTTC	1 cut(s)	101
SatI	GCNGC	7 cut(s)	888, 1279, 1353, 1827, 1960, 2185, 2238
Sau96I	GGNCC	3 cut(s)	312, 1648, 1796
ScaI	AGTACT	1 cut(s)	1597
SchI	GAGTC	2 cut(s)	608, 1804
ScrFI	CCNGG	8 cut(s)	558, 789, 1376, 1409, 1481, 1802, 1878, 2037
SduI	GDGCHC	7 cut(s)	329, 445, 846, 1060, 1117, 1851, 2276
SfcI	CTRYAG	4 cut(s)	407, 762, 1196, 1560
Sfr274I	CTCGAG	1 cut(s)	1911
SfuI	TTCGAA	1 cut(s)	1669
SinI	GGWCC	1 cut(s)	1796
SlaI	CTCGAG	1 cut(s)	1911
SmiI	ATTTAAAT	1 cut(s)	489
SmlI	CTYRAG	6 cut(s)	171, 271, 476, 1911, 2006, 2332
SmoI	CTYRAG	6 cut(s)	171, 271, 476, 1911, 2006, 2332
Sse9I	AATT	9 cut(s)	393, 451, 607, 807, 1032, 1430, 1520, 1675, 1890
SseBI	AGGCCT	1 cut(s)	256
SsiI	CCGC	3 cut(s)	673, 888, 1960
SspI	AATATT	1 cut(s)	1392
SspMI	CTAG	9 cut(s)	530, 645, 821, 927, 1118, 1493, 1830, 2103, 2174
SstI	GAGCTC	1 cut(s)	846
StuI	AGGCCT	1 cut(s)	256
StyD4I	CCNGG	8 cut(s)	556, 787, 1374, 1407, 1479, 1800, 1876, 2035
StyI	CCWWGG	1 cut(s)	529
SwaI	ATTTAAAT	1 cut(s)	489
TaiI	ACGT	4 cut(s)	836, 1158, 1752, 1854
TaqI	TCGA	3 cut(s)	741, 1669, 1912
TasI	AATT	9 cut(s)	393, 451, 607, 807, 1032, 1430, 1520, 1675, 1890
TatI	WGTACW	4 cut(s)	954, 1595, 1919, 2202
TauI	GCSGC	2 cut(s)	890, 1962
TfiI	GAWTC	7 cut(s)	506, 572, 653, 661, 1209, 1245, 1730
TscAI	CASTG	6 cut(s)	193, 329, 607, 1205, 2047, 2202
TseFI	GTSAC	4 cut(s)	544, 630, 1475, 2193
TseI	GCWGC	5 cut(s)	1278, 1352, 1826, 2184, 2237
Tsp45I	GTSAC	4 cut(s)	544, 630, 1475, 2193
TspDTI	ATGAA	6 cut(s)	723, 1237, 1436, 1443, 1808, 2281
TspGWI	ACGGA	3 cut(s)	806, 1528, 1612
TspRI	CASTG	6 cut(s)	193, 329, 607, 1205, 2047, 2202
Tth111I	GACNNNGTC	1 cut(s)	1473
Van91I	CCANNNNNTGG	1 cut(s)	1414
VpaK11BI	GGWCC	1 cut(s)	1796
VspI	ATTAAT	1 cut(s)	2138
XapI	RAATTY	1 cut(s)	607
XbaI	TCTAGA	2 cut(s)	820, 1117
XceI	RCATGY	1 cut(s)	2021
XhoI	CTCGAG	1 cut(s)	1911
XmaJI	CCTAGG	1 cut(s)	529
XmiI	GTMKAC	2 cut(s)	406, 923
XmnI	GAANNNNTTC	1 cut(s)	364
XspI	CTAG	9 cut(s)	530, 645, 821, 927, 1118, 1493, 1830, 2103, 2174
ZrmI	AGTACT	1 cut(s)	1597
Zsp2I	ATGCAT	3 cut(s)	797, 1948, 2137

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.