Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr13

Orthogroup ID

OG0002791

Physical Location & Seq

Genomic Coordinates Forward (+)

25668283 .. 25672881

Gene Structure 4599 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

MD13G1245000.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 996 bp

ATGCGAAATTTAACAAAAGGAAAACAAACCGCTTCAAATACTCAAATCAAAACTGACTTTTGTGTTGCCCGCAATCTCTGCTTGCCTGCTCACTTTCTCTGTTTCTCAGTCGATCAAGATACAGTTATGGCCTTGAGAACAGTGACGAGGAAGTTAGGGAGTAAACTATTACACAGGTTTTCTCCTGCTGCTCTATTGCATTCACATGCTACAAGCTTTGGATTTAAAGAAGTAAGGGAAGAGGAAAAAAGTCGAATGGTTGGTAATGTCTTCAGCAATGTTGCTTCGAACTATGATCTAATGAATGATGTGATGAGTGCAGGATTACACAGATTATGGAAGGATAGATTAGTTTCCAAACTGAATCCTTTCCCTGGAATGAAGCATCTTGATGTGGCTGGTGGGACAGGTGATGTTGCCTTCAGAATACTAGACTCAGTAAACAGTGTCAGACGTAGAGCTATGCAAGATGTTCTCGAAGATGATTTACAAGAAGAAACTAAGATTTATGTATGTGACATAAACCCTGACATGTTGAATGTCGGTAAAAAGCAGGCGTTGGAGAGAGGTATTGGAGAAGACAAATCCCTTCTATGGGTGGAGGGAGACGCAGAAGCCTTGTCTTTTGGAGATAATACAATGGATGGTTACACAATTGCATTTGGAATTAGGAATGTTACACACATAGAGAAAGTGTTGTCTGAAGCCTATCGAGTGCTAAAGCGTGGAGGAAGGTTCCTGTGCCTTGAACTGAGCCATGTAGACATTCCTGTATTCAAAGAATTCTATGATTTTTACTCATTCTCAGTCATTCCAGCCCTGGGAGAGCTCGTTGCAGGGGATAGAAAATCTTACCAGTACTTGGTAGAGAGTATTCGTCGTTTCCCCCCACAGGAGAAGTTTGCGTCGATGATTGCGGATGCAGGATTCCAGCAGGTTGAATATGAGAACCTTGTTGGAGGTGTAGTTGCTATTCATTCGGGGTTGAAAGTTTAG

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005739 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044424 GO:0044444 GO:0044464

KEGG Pathways

Metabolic & Signaling

ko00130 ko01100 ko01110 map00130 map01100 map01110

Pfam Domains

Protein Families

Ubie_methyltran

Protein Analysis

332

Amino Acids

37.14

Weight (kDa)

6.76

Isoelectric Point (pI)

34.93

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Ubie_methyltran	PF01209	82 - 330	2.5e-84	ubiE/COQ5 methyltransferase family
Methyltransf_31	PF13847	125 - 252	1.1e-06	Methyltransferase domain
Methyltransf_12	PF08242	130 - 246	1.6e-06	Methyltransferase domain
Methyltransf_25	PF13649	166 - 244	1.2e-10	Methyltransferase domain
Methyltransf_11	PF08241	167 - 248	1.5e-12	Methyltransferase domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0002791)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT5G57300 AT5G57300 AT5G57300 AT5G57300
fragaria_vesca	FvH4_4g07660 FvH4_4g07660 FvH4_4g07660 FvH4_4g07660 FvH4_4g07660 FvH4_4g07660 FvH4_4g07660 FvH4_4g07660
malus_domestica	MD03G1035800.v1.1 MD03G1036000.v1.1 MD13G1245000.v1.1 MD16G1253300.v1.1
prunus_persica	Prupe.1G079700_v2.0.a1 Prupe.1G079700_v2.0.a1
pyrus_communis	pycom13g21600
rosa_chinensis	RchiOBHm_Chr4g0400331 RchiOBHm_Chr4g0400411 RchiOBHm_Chr4g0400471 RchiOBHm_Chr4g0400591 RchiOBHm_Chr4g0400601 RchiOBHm_Chr4g0400651 RchiOBHm_Chr4g0400661
rosa_laevigata	RLG00000009189
rosa_multiflora	Rmu_sc0003957.1_g000001
rosa_roxburghii	Rroxscaffold_5G00345430
rosa_rugosa	Rorug04G0024900 Rorug04G0025000
rosa_samantha	Rh4AG101200 Rh4AG101300 Rh4AG102600 Rh4BG097900 Rh4CG110900 Rh4DG094600 Rh7BG256500
rosa_wichuraiana	Rw4G008310

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
Acc36I	ACCTGC	1 cut(s)	925
AccB7I	CCANNNNNTGG	1 cut(s)	862
AccI	GTMKAC	1 cut(s)	762
AciI	CCGC	3 cut(s)	30, 70, 917
AcsI	RAATTY	2 cut(s)	7, 782
AcuI	CTGAAG	3 cut(s)	256, 406, 723
AfaI	GTAC	1 cut(s)	860
AfiI	CCNNNNNNNGG	6 cut(s)	374, 594, 595, 821, 862, 892
AflIII	ACRYGT	1 cut(s)	531
AgsI	TTSAA	6 cut(s)	36, 538, 749, 778, 941, 988
AjnI	CCWGG	2 cut(s)	373, 819
AluBI	AGCT	3 cut(s)	216, 461, 829
AluI	AGCT	3 cut(s)	216, 461, 829
Alw21I	GWGCWC	1 cut(s)	831
Alw26I	GTCTC	1 cut(s)	600
AoxI	GGCC	1 cut(s)	129
ApeKI	GCWGC	1 cut(s)	188
ApoI	RAATTY	2 cut(s)	7, 782
Asp700I	GAANNNNTTC	1 cut(s)	368
AsuHPI	GGTGA	1 cut(s)	422
AsuII	TTCGAA	1 cut(s)	287
BanII	GRGCYC	1 cut(s)	831
BarI	GAAGNNNNNNTAC	2 cut(s)	471, 503
BbsI	GAAGAC	2 cut(s)	262, 585
Bbv12I	GWGCWC	1 cut(s)	831
BbvI	GCAGC	1 cut(s)	175
BccI	CCATC	1 cut(s)	638
BciT130I	CCWGG	2 cut(s)	375, 821
BcoDI	GTCTC	1 cut(s)	600
BfaI	CTAG	1 cut(s)	431
BfuAI	ACCTGC	1 cut(s)	925
BisI	GCNGC	1 cut(s)	189
BlsI	GCNGC	1 cut(s)	190
BmcAI	AGTACT	1 cut(s)	860
Bme1390I	CCNGG	2 cut(s)	375, 821
BmiI	GGNNCC	1 cut(s)	737
BmrFI	CCNGG	2 cut(s)	375, 821
BmsI	GCATC	2 cut(s)	394, 910
BpiI	GAAGAC	2 cut(s)	262, 585
Bpu14I	TTCGAA	1 cut(s)	287
BpuEI	CTTGAG	1 cut(s)	154
BsaJI	CCNNGG	3 cut(s)	373, 819, 820
BsaXI	ACNNNNNCTCC	4 cut(s)	816, 846, 951, 981
Bsc4I	CCNNNNNNNGG	6 cut(s)	374, 594, 595, 821, 862, 892
Bse1I	ACTGG	1 cut(s)	856
Bse3DI	GCAATG	1 cut(s)	283
BseBI	CCWGG	2 cut(s)	375, 821
BseDI	CCNNGG	3 cut(s)	373, 819, 820
BseGI	GGATG	2 cut(s)	649, 925
BseLI	CCNNNNNNNGG	6 cut(s)	374, 594, 595, 821, 862, 892
BseMI	GCAATG	1 cut(s)	283
BseMII	CTCAG	4 cut(s)	120, 450, 743, 819
BseNI	ACTGG	1 cut(s)	856
BseXI	GCAGC	1 cut(s)	175
BsgI	GTGCAG	1 cut(s)	339
BshFI	GGCC	1 cut(s)	131
BsiHKAI	GWGCWC	1 cut(s)	831
BslFI	GGGAC	1 cut(s)	418
BslI	CCNNNNNNNGG	6 cut(s)	374, 594, 595, 821, 862, 892
BsmAI	GTCTC	1 cut(s)	600
BsmBI	CGTCTC	1 cut(s)	600
BsmFI	GGGAC	1 cut(s)	418
BsmI	GAATGC	1 cut(s)	199
BsnI	GGCC	1 cut(s)	131
Bsp119I	TTCGAA	1 cut(s)	287
Bsp1286I	GDGCHC	1 cut(s)	831
Bsp143I	GATC	2 cut(s)	112, 295
BspACI	CCGC	3 cut(s)	30, 70, 917
BspANI	GGCC	1 cut(s)	131
BspCNI	CTCAG	4 cut(s)	119, 449, 744, 818
BspLI	GGNNCC	1 cut(s)	737
BspMI	ACCTGC	1 cut(s)	925
BspT104I	TTCGAA	1 cut(s)	287
BsrDI	GCAATG	1 cut(s)	283
BsrI	ACTGG	1 cut(s)	856
BssECI	CCNNGG	3 cut(s)	373, 819, 820
BssMI	GATC	2 cut(s)	112, 295
Bst2UI	CCWGG	2 cut(s)	375, 821
Bst4CI	ACNGT	3 cut(s)	124, 142, 446
Bst6I	CTCTTC	1 cut(s)	234
BstAPI	GCANNNNNTGC	1 cut(s)	78
BstBI	TTCGAA	1 cut(s)	287
BstC8I	GCNNGC	4 cut(s)	70, 83, 87, 555
BstDEI	CTNAG	5 cut(s)	106, 436, 501, 752, 805
BstF5I	GGATG	2 cut(s)	649, 925
BstKTI	GATC	2 cut(s)	115, 298
BstMAI	GTCTC	1 cut(s)	600
BstMBI	GATC	2 cut(s)	112, 295
BstMWI	GCNNNNNNNGC	1 cut(s)	78
BstNI	CCWGG	2 cut(s)	375, 821
BstNSI	RCATGY	2 cut(s)	209, 535
BstSCI	CCNGG	2 cut(s)	373, 819
BstV1I	GCAGC	1 cut(s)	175
BstV2I	GAAGAC	2 cut(s)	262, 585
BsuRI	GGCC	1 cut(s)	131
BtsCI	GGATG	2 cut(s)	649, 925
BtsIMutI	CAGTG	2 cut(s)	147, 451
BveI	ACCTGC	1 cut(s)	925
Cac8I	GCNNGC	4 cut(s)	70, 83, 87, 555
CseI	GACGC	2 cut(s)	617, 894
Csp6I	GTAC	1 cut(s)	859
CviAII	CATG	3 cut(s)	206, 532, 758
CviJI	RGCY	9 cut(s)	131, 216, 398, 461, 617, 707, 756, 818, 829
CviKI_1	RGCY	9 cut(s)	131, 216, 398, 461, 617, 707, 756, 818, 829
CviQI	GTAC	1 cut(s)	859
DdeI	CTNAG	5 cut(s)	106, 436, 501, 752, 805
DpnI	GATC	2 cut(s)	114, 297
DpnII	GATC	2 cut(s)	112, 295
DraI	TTTAAA	1 cut(s)	226
Eam1104I	CTCTTC	1 cut(s)	234
EarI	CTCTTC	1 cut(s)	234
Ecl136II	GAGCTC	1 cut(s)	829
Eco24I	GRGCYC	1 cut(s)	831
Eco53kI	GAGCTC	1 cut(s)	829
Eco57I	CTGAAG	3 cut(s)	256, 406, 723
EcoICRI	GAGCTC	1 cut(s)	829
EcoRI	GAATTC	1 cut(s)	782
EcoRII	CCWGG	2 cut(s)	373, 819
EcoT38I	GRGCYC	1 cut(s)	831
Esp3I	CGTCTC	1 cut(s)	600
FaeI	CATG	3 cut(s)	209, 535, 761
FaqI	GGGAC	1 cut(s)	418
FatI	CATG	3 cut(s)	205, 531, 757
FauI	CCCGC	1 cut(s)	77
FblI	GTMKAC	1 cut(s)	762
Fnu4HI	GCNGC	1 cut(s)	189
FokI	GGATG	2 cut(s)	656, 932
FriOI	GRGCYC	1 cut(s)	831
Fsp4HI	GCNGC	1 cut(s)	189
FspBI	CTAG	1 cut(s)	431
GluI	GCNGC	1 cut(s)	189
HaeIII	GGCC	1 cut(s)	131
HgaI	GACGC	2 cut(s)	617, 894
Hin1II	CATG	3 cut(s)	209, 535, 761
HindIII	AAGCTT	1 cut(s)	214
HinfI	GANTC	3 cut(s)	364, 434, 927
HphI	GGTGA	1 cut(s)	422
Hpy166II	GTNNAC	3 cut(s)	164, 442, 763
Hpy188I	TCNGA	3 cut(s)	425, 452, 703
Hpy188III	TCNNGA	3 cut(s)	116, 389, 476
Hpy8I	GTNNAC	3 cut(s)	164, 442, 763
Hpy99I	CGWCG	2 cut(s)	882, 910
HpyAV	CCTTC	4 cut(s)	334, 430, 599, 726
HpyCH4III	ACNGT	3 cut(s)	124, 142, 446
HpyCH4IV	ACGT	1 cut(s)	454
HpyCH4V	TGCA	6 cut(s)	199, 320, 466, 659, 836, 923
HpyF10VI	GCNNNNNNNGC	1 cut(s)	78
HpyF3I	CTNAG	5 cut(s)	106, 436, 501, 752, 805
HpySE526I	ACGT	1 cut(s)	454
Hsp92II	CATG	3 cut(s)	209, 535, 761
Kzo9I	GATC	2 cut(s)	112, 295
Lsp1109I	GCAGC	1 cut(s)	175
LweI	GCATC	2 cut(s)	394, 910
MaeI	CTAG	1 cut(s)	431
MaeII	ACGT	1 cut(s)	454
MaeIII	GTNAC	4 cut(s)	142, 515, 647, 676
MalI	GATC	2 cut(s)	114, 297
MboI	GATC	2 cut(s)	112, 295
MboII	GAAGA	5 cut(s)	251, 262, 491, 506, 590
MfeI	CAATTG	1 cut(s)	654
MhlI	GDGCHC	1 cut(s)	831
MluCI	AATT	4 cut(s)	7, 654, 666, 782
MlyI	GAGTC	1 cut(s)	428
MmeI	TCCRAC	2 cut(s)	540, 937
MnlI	CCTC	6 cut(s)	141, 235, 560, 595, 722, 953
MroXI	GAANNNNTTC	1 cut(s)	368
MseI	TTAA	2 cut(s)	11, 225
MslI	CAYNNNNRTG	2 cut(s)	204, 390
MspR9I	CCNGG	2 cut(s)	375, 821
MunI	CAATTG	1 cut(s)	654
Mva1269I	GAATGC	1 cut(s)	199
MvaI	CCWGG	2 cut(s)	375, 821
MwoI	GCNNNNNNNGC	1 cut(s)	78
NdeII	GATC	2 cut(s)	112, 295
NlaIII	CATG	3 cut(s)	209, 535, 761
NlaIV	GGNNCC	1 cut(s)	737
NmuCI	GTSAC	2 cut(s)	142, 515
NspI	RCATGY	2 cut(s)	209, 535
NspV	TTCGAA	1 cut(s)	287
PasI	CCCWGGG	1 cut(s)	820
PciI	ACATGT	1 cut(s)	531
PctI	GAATGC	1 cut(s)	199
PdmI	GAANNNNTTC	1 cut(s)	368
PfeI	GAWTC	2 cut(s)	364, 927
PflMI	CCANNNNNTGG	1 cut(s)	862
PkrI	GCNGC	1 cut(s)	190
PleI	GAGTC	1 cut(s)	428
PpsI	GAGTC	1 cut(s)	428
PscI	ACATGT	1 cut(s)	531
Psp124BI	GAGCTC	1 cut(s)	831
Psp6I	CCWGG	2 cut(s)	373, 819
PspGI	CCWGG	2 cut(s)	373, 819
PspN4I	GGNNCC	1 cut(s)	737
RsaI	GTAC	1 cut(s)	860
RsaNI	GTAC	1 cut(s)	859
RseI	CAYNNNNRTG	2 cut(s)	204, 390
SacI	GAGCTC	1 cut(s)	831
SaqAI	TTAA	2 cut(s)	11, 225
SatI	GCNGC	1 cut(s)	189
Sau3AI	GATC	2 cut(s)	112, 295
ScaI	AGTACT	1 cut(s)	860
SchI	GAGTC	1 cut(s)	428
ScrFI	CCNGG	2 cut(s)	375, 821
SduI	GDGCHC	1 cut(s)	831
SfaNI	GCATC	2 cut(s)	394, 910
SfuI	TTCGAA	1 cut(s)	287
SmiMI	CAYNNNNRTG	2 cut(s)	204, 390
SmlI	CTYRAG	1 cut(s)	133
SmoI	CTYRAG	1 cut(s)	133
Sse9I	AATT	4 cut(s)	7, 654, 666, 782
SsiI	CCGC	3 cut(s)	30, 70, 917
SspMI	CTAG	1 cut(s)	431
SstI	GAGCTC	1 cut(s)	831
StyD4I	CCNGG	2 cut(s)	373, 819
TaaI	ACNGT	3 cut(s)	124, 142, 446
TaiI	ACGT	1 cut(s)	457
TaqI	TCGA	6 cut(s)	111, 253, 287, 477, 712, 908
TasI	AATT	4 cut(s)	7, 654, 666, 782
TatI	WGTACW	1 cut(s)	858
TfiI	GAWTC	2 cut(s)	364, 927
Tru1I	TTAA	2 cut(s)	11, 225
Tru9I	TTAA	2 cut(s)	11, 225
TscAI	CASTG	2 cut(s)	147, 451
TseFI	GTSAC	2 cut(s)	142, 515
TseI	GCWGC	1 cut(s)	188
Tsp45I	GTSAC	2 cut(s)	142, 515
TspDTI	ATGAA	3 cut(s)	317, 395, 965
TspRI	CASTG	2 cut(s)	147, 451
Van91I	CCANNNNNTGG	1 cut(s)	862
XapI	RAATTY	2 cut(s)	7, 782
XceI	RCATGY	2 cut(s)	209, 535
XmiI	GTMKAC	1 cut(s)	762
XmnI	GAANNNNTTC	1 cut(s)	368
XspI	CTAG	1 cut(s)	431
ZrmI	AGTACT	1 cut(s)	860

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.