RchiOBHm_Chr4g0400601

Methyltransferase required for the conversion of 2- polyprenyl-6-methoxy-1,4-benzoquinol (DDMQH2) to 2-polyprenyl-3- methyl-6-methoxy-1,4-benzoquinol (DMQH2)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
4
OG0002791
Physical Location & Seq
Reverse (-)
18311285 .. 18313608
2324 bp
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UTR
Exon/CDS
Intron
PRQ37259

Sequence Viewer

Length: 534 bp
ATGGTTGGTAATGTCTTCAGCAATGTTGCTTCAAACTATGATACAATGAACATTTTGATGAGTGCAGGATTACACAGATTATGGAAGGATAGATTGGTTTCAAAACTGAATCCTTTTCCTGGAATGAACCATCTTGACGTGGCTGGTGGAACAGGTGATGTTGCTTTCAGAATACTAGACAGTATAAACAGCATCAAACGTAGAGCTTTGCTGAATGTTCTTGAAGATGATTTACAGGAAGAAACTAAGGTGTATGTATGTGACACCAATCCCAACATGTTGAGTGTTGGTAAAAATCGGGCCTTGCAGAGAGGTCGTGGAGAAGATAAATCCCTTCTTTGGGTGGAGGGAGATGCAGAAGCCCTGAGTTTCGGAGATAATACAATGGATGGTTACAAAATTGCATTTGGGATCCGGAATGTCACACACATAGAGAAAGCACTTTCTGAAGCTTATAGGGTACTAAAACGGGGAGGAAGGTTCCTGTGCCTTGAACTCAGCCATGTAGATGTACCTGTATTCAAAGACTTGTAA

Protein Analysis

177

Amino Acids

19.74

Weight (kDa)

6.42

Isoelectric Point (pI)

36.17

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Ubie_methyltran PF01209 2 - 175 2.6e-48 ubiE/COQ5 methyltransferase family
Methyltransf_12 PF08242 45 - 161 3.4e-06 Methyltransferase domain
Methyltransf_25 PF13649 78 - 159 9e-10 Methyltransferase domain
Methyltransf_11 PF08241 82 - 163 3.3e-12 Methyltransferase domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccIII TCCGGA 1 cut(s) 414
AclWI GGATC 2 cut(s) 406, 419
AcuI CTGAAG 1 cut(s) 468
AfaI GTAC 2 cut(s) 462, 513
AfiI CCNNNNNNNGG 3 cut(s) 119, 339, 340
AflIII ACRYGT 1 cut(s) 276
AgsI TTSAA 5 cut(s) 33, 102, 224, 494, 523
AjiI CACGTC 1 cut(s) 139
AjnI CCWGG 1 cut(s) 118
AjuI GAANNNNNNNTTGG 2 cut(s) 77, 109
AluBI AGCT 2 cut(s) 206, 452
AluI AGCT 2 cut(s) 206, 452
AlwI GGATC 2 cut(s) 406, 419
Aor13HI TCCGGA 1 cut(s) 414
AoxI GGCC 1 cut(s) 300
AspS9I GGNCC 1 cut(s) 300
AsuHPI GGTGA 1 cut(s) 167
BamHI GGATCC 1 cut(s) 411
BarI GAAGNNNNNNTAC 2 cut(s) 216, 248
BbsI GAAGAC 1 cut(s) 7
BccI CCATC 2 cut(s) 138, 383
BcgI CGANNNNNNTGC 2 cut(s) 296, 330
BciT130I CCWGG 1 cut(s) 120
BfaI CTAG 1 cut(s) 176
Bme1390I CCNGG 1 cut(s) 120
BmgBI CACGTC 1 cut(s) 139
BmgT120I GGNCC 1 cut(s) 300
BmiI GGNNCC 2 cut(s) 413, 482
BmrFI CCNGG 1 cut(s) 120
BmsI GCATC 2 cut(s) 201, 343
BpiI GAAGAC 1 cut(s) 7
BsaWI WCCGGW 1 cut(s) 414
Bsc4I CCNNNNNNNGG 3 cut(s) 119, 339, 340
Bse3DI GCAATG 1 cut(s) 28
BseAI TCCGGA 1 cut(s) 414
BseBI CCWGG 1 cut(s) 120
BseGI GGATG 1 cut(s) 394
BseLI CCNNNNNNNGG 3 cut(s) 119, 339, 340
BseMI GCAATG 1 cut(s) 28
BseMII CTCAG 2 cut(s) 356, 511
BsgI GTGCAG 1 cut(s) 84
BshFI GGCC 1 cut(s) 302
BsiSI CCGG 1 cut(s) 415
BslI CCNNNNNNNGG 3 cut(s) 119, 339, 340
BsnI GGCC 1 cut(s) 302
Bsp13I TCCGGA 1 cut(s) 414
Bsp143I GATC 1 cut(s) 411
BspANI GGCC 1 cut(s) 302
BspCNI CTCAG 2 cut(s) 357, 510
BspEI TCCGGA 1 cut(s) 414
BspLI GGNNCC 2 cut(s) 413, 482
BspPI GGATC 2 cut(s) 406, 419
BsrDI GCAATG 1 cut(s) 28
BssMI GATC 1 cut(s) 411
Bst2UI CCWGG 1 cut(s) 120
Bst4CI ACNGT 1 cut(s) 182
BstDEI CTNAG 3 cut(s) 246, 365, 497
BstF5I GGATG 1 cut(s) 394
BstKTI GATC 1 cut(s) 414
BstMBI GATC 1 cut(s) 411
BstNI CCWGG 1 cut(s) 120
BstNSI RCATGY 1 cut(s) 280
BstSCI CCNGG 1 cut(s) 118
BstV2I GAAGAC 1 cut(s) 7
BstX2I RGATCY 1 cut(s) 411
BstYI RGATCY 1 cut(s) 411
BsuRI GGCC 1 cut(s) 302
BtrI CACGTC 1 cut(s) 139
BtsCI GGATG 1 cut(s) 394
Cfr13I GGNCC 1 cut(s) 300
Csp6I GTAC 2 cut(s) 461, 512
CviAII CATG 2 cut(s) 277, 503
CviJI RGCY 6 cut(s) 143, 206, 302, 362, 452, 501
CviKI_1 RGCY 6 cut(s) 143, 206, 302, 362, 452, 501
CviQI GTAC 2 cut(s) 461, 512
DdeI CTNAG 3 cut(s) 246, 365, 497
DpnI GATC 1 cut(s) 413
DpnII GATC 1 cut(s) 411
Eco57I CTGAAG 1 cut(s) 468
EcoRII CCWGG 1 cut(s) 118
FaeI CATG 2 cut(s) 280, 506
FaiI YATR 9 cut(s) 39, 82, 185, 255, 259, 278, 431, 456, 504
FatI CATG 2 cut(s) 276, 502
FokI GGATG 1 cut(s) 401
FspBI CTAG 1 cut(s) 176
HaeIII GGCC 1 cut(s) 302
HapII CCGG 1 cut(s) 415
Hin1II CATG 2 cut(s) 280, 506
HindIII AAGCTT 1 cut(s) 450
HinfI GANTC 1 cut(s) 109
HpaII CCGG 1 cut(s) 415
HphI GGTGA 1 cut(s) 167
Hpy188I TCNGA 3 cut(s) 170, 374, 448
Hpy188III TCNNGA 3 cut(s) 134, 221, 415
HpyAV CCTTC 3 cut(s) 79, 344, 471
HpyCH4III ACNGT 1 cut(s) 182
HpyCH4IV ACGT 2 cut(s) 138, 199
HpyCH4V TGCA 4 cut(s) 65, 307, 356, 404
HpyF3I CTNAG 3 cut(s) 246, 365, 497
HpySE526I ACGT 2 cut(s) 138, 199
Hsp92II CATG 2 cut(s) 280, 506
Kpn2I TCCGGA 1 cut(s) 414
Kzo9I GATC 1 cut(s) 411
LweI GCATC 2 cut(s) 201, 343
MaeI CTAG 1 cut(s) 176
MaeII ACGT 2 cut(s) 138, 199
MaeIII GTNAC 3 cut(s) 260, 392, 421
MalI GATC 1 cut(s) 413
MboI GATC 1 cut(s) 411
MboII GAAGA 4 cut(s) 7, 236, 251, 335
MflI RGATCY 1 cut(s) 411
MluCI AATT 1 cut(s) 399
MnlI CCTC 3 cut(s) 305, 340, 467
MroI TCCGGA 1 cut(s) 414
MslI CAYNNNNRTG 2 cut(s) 56, 507
MspI CCGG 1 cut(s) 415
MspR9I CCNGG 1 cut(s) 120
MvaI CCWGG 1 cut(s) 120
NdeII GATC 1 cut(s) 411
NlaIII CATG 2 cut(s) 280, 506
NlaIV GGNNCC 2 cut(s) 413, 482
NmuCI GTSAC 2 cut(s) 260, 421
NspI RCATGY 1 cut(s) 280
PciI ACATGT 1 cut(s) 276
PfeI GAWTC 1 cut(s) 109
PfoI TCCNGGA 1 cut(s) 118
PscI ACATGT 1 cut(s) 276
Psp6I CCWGG 1 cut(s) 118
PspGI CCWGG 1 cut(s) 118
PspN4I GGNNCC 2 cut(s) 413, 482
PspPI GGNCC 1 cut(s) 300
PsuI RGATCY 1 cut(s) 411
RsaI GTAC 2 cut(s) 462, 513
RsaNI GTAC 2 cut(s) 461, 512
RseI CAYNNNNRTG 2 cut(s) 56, 507
Sau3AI GATC 1 cut(s) 411
Sau96I GGNCC 1 cut(s) 300
ScrFI CCNGG 1 cut(s) 120
SetI ASST 9 cut(s) 141, 157, 202, 208, 252, 316, 454, 482, 517
SfaNI GCATC 2 cut(s) 201, 343
SmiMI CAYNNNNRTG 2 cut(s) 56, 507
Sse9I AATT 1 cut(s) 399
SspMI CTAG 1 cut(s) 176
StyD4I CCNGG 1 cut(s) 118
TaaI ACNGT 1 cut(s) 182
TaiI ACGT 2 cut(s) 141, 202
TasI AATT 1 cut(s) 399
TfiI GAWTC 1 cut(s) 109
TseFI GTSAC 2 cut(s) 260, 421
Tsp45I GTSAC 2 cut(s) 260, 421
TspDTI ATGAA 2 cut(s) 62, 140
XceI RCATGY 1 cut(s) 280
XspI CTAG 1 cut(s) 176
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.