RchiOBHm_Chr4g0400471

Methyltransferase required for the conversion of 2- polyprenyl-6-methoxy-1,4-benzoquinol (DDMQH2) to 2-polyprenyl-3- methyl-6-methoxy-1,4-benzoquinol (DMQH2)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
4
OG0002791
Physical Location & Seq
Reverse (-)
18048983 .. 18054237
5255 bp
Loading structure...
UTR
Exon/CDS
Intron
PRQ37249

Sequence Viewer

Length: 870 bp
ATGGCCTTGAGAACAGTGACGAGGAAGTTAGGGAGTAAACTGTCACCCAGGTTTTGTCCTGCATCTCTATTGCATTCTCATGCCACAAGCTTTGGATTCAAGGAAGTACGGGAAGATGAAAAAAGTCAAATGGTTGGTAATGTCTTCAGCAATGTTGCTTCAAACTATGATACAATGAATGATTTGATGAGCGCAGGATTACACAGATTATGGAAGGATAGATTGGTTTCCAAATTGAATCCTTTTCCTGGAATGAACCATCTTGATGTGGCTGGTGGAACAGGTGATGTTGCTTTCAGAATACTAGACAGTATAAACAGCATCAAACGTAGAGCTTTGCTGAATGTTCTTGAAGATGATTTTCAGGAAGAAACTAAGGTGTATGTATGTGACATCAATCCCAACATGTTGAATGTTGGTAAAAAGCGGGCCTTGCTGAGAGGTCTTGGAGAAGACAAATCCCTTCTATGGGTGGAGGGAGATGCAGAAGCCCTGAGTTTCGGAGATAATACAATGGATGGTTACACAATTGCATTTGGGATCCGGAATGTCACACACATAGAGAAAGCACTTTCTGAAGCTTATAGGGTACTGAAACGGGGAGGAAGGTTCCTGTGCCTTGAACTCAGCCATGTAGAGGTACCTGTATTCAAAGACTTCTATGACTTTTATTCATTCTCAGTCATTCCCACCCTAGGAGAGCTAGTTGCAGGGGATAGAGAATCTTATCAGTACTTGGTAGAGAGTATTCGTCGTTTTCCACCACAGGAGACATTTGCTTCAATGATTGCAGATGCAGGTTTCCAGAAGGTTGAGTATGAAAATCTAGTTGGAGGAGTGGTAGCGATTCATTCAGGATTGAAAATTTAG

Protein Analysis

289

Amino Acids

32.33

Weight (kDa)

6.19

Isoelectric Point (pI)

35.79

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Ubie_methyltran PF01209 39 - 288 1.3e-84 ubiE/COQ5 methyltransferase family
Methyltransf_31 PF13847 83 - 210 2.9e-06 Methyltransferase domain
Methyltransf_12 PF08242 88 - 204 1.8e-06 Methyltransferase domain
Methyltransf_25 PF13649 118 - 202 5.5e-11 Methyltransferase domain
Methyltransf_11 PF08241 125 - 206 2.1e-13 Methyltransferase domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 788
Acc65I GGTACC 1 cut(s) 640
AccB1I GGYRCC 1 cut(s) 640
AccIII TCCGGA 1 cut(s) 543
AciI CCGC 1 cut(s) 427
AclWI GGATC 2 cut(s) 535, 548
AcsI RAATTY 1 cut(s) 864
AcuI CTGAAG 2 cut(s) 130, 597
AfaI GTAC 4 cut(s) 108, 591, 642, 734
AfiI CCNNNNNNNGG 5 cut(s) 248, 468, 469, 637, 695
AflIII ACRYGT 1 cut(s) 405
AgsI TTSAA 9 cut(s) 100, 162, 238, 353, 412, 623, 652, 783, 862
AjnI CCWGG 2 cut(s) 47, 247
AjuI GAANNNNNNNTTGG 4 cut(s) 206, 238, 813, 845
AluBI AGCT 4 cut(s) 90, 335, 581, 703
AluI AGCT 4 cut(s) 90, 335, 581, 703
Alw26I GTCTC 1 cut(s) 764
AlwI GGATC 2 cut(s) 535, 548
Aor13HI TCCGGA 1 cut(s) 543
AoxI GGCC 2 cut(s) 3, 429
ApoI RAATTY 1 cut(s) 864
Asp718I GGTACC 1 cut(s) 640
AspA2I CCTAGG 1 cut(s) 694
AspLEI GCGC 1 cut(s) 194
AspS9I GGNCC 1 cut(s) 429
AsuHPI GGTGA 2 cut(s) 36, 296
AvrII CCTAGG 1 cut(s) 694
BamHI GGATCC 1 cut(s) 540
BanI GGYRCC 1 cut(s) 640
BbsI GAAGAC 2 cut(s) 136, 459
BccI CCATC 2 cut(s) 267, 512
BciT130I CCWGG 2 cut(s) 49, 249
BcoDI GTCTC 1 cut(s) 764
BfaI CTAG 4 cut(s) 305, 695, 704, 827
BfuAI ACCTGC 1 cut(s) 788
BlnI CCTAGG 1 cut(s) 694
BmcAI AGTACT 1 cut(s) 734
Bme1390I CCNGG 2 cut(s) 49, 249
BmgT120I GGNCC 1 cut(s) 429
BmiI GGNNCC 3 cut(s) 542, 611, 642
BmrFI CCNGG 2 cut(s) 49, 249
BmsI GCATC 4 cut(s) 71, 330, 472, 784
BpiI GAAGAC 2 cut(s) 136, 459
BpuEI CTTGAG 1 cut(s) 28
BsaJI CCNNGG 2 cut(s) 47, 694
BsaWI WCCGGW 1 cut(s) 543
BsaXI ACNNNNNCTCC 4 cut(s) 690, 720, 825, 855
Bsc4I CCNNNNNNNGG 5 cut(s) 248, 468, 469, 637, 695
Bse3DI GCAATG 1 cut(s) 157
BseAI TCCGGA 1 cut(s) 543
BseBI CCWGG 2 cut(s) 49, 249
BseDI CCNNGG 2 cut(s) 47, 694
BseGI GGATG 1 cut(s) 523
BseLI CCNNNNNNNGG 5 cut(s) 248, 468, 469, 637, 695
BseMI GCAATG 1 cut(s) 157
BseMII CTCAG 4 cut(s) 428, 485, 640, 693
BseRI GAGGAG 1 cut(s) 849
BshFI GGCC 2 cut(s) 5, 431
BshNI GGYRCC 1 cut(s) 640
BsiSI CCGG 1 cut(s) 544
BslI CCNNNNNNNGG 5 cut(s) 248, 468, 469, 637, 695
BsmAI GTCTC 1 cut(s) 764
BsmI GAATGC 1 cut(s) 73
BsnI GGCC 2 cut(s) 5, 431
Bsp13I TCCGGA 1 cut(s) 543
Bsp143I GATC 1 cut(s) 540
BspACI CCGC 1 cut(s) 427
BspANI GGCC 2 cut(s) 5, 431
BspCNI CTCAG 4 cut(s) 429, 486, 639, 692
BspEI TCCGGA 1 cut(s) 543
BspLI GGNNCC 3 cut(s) 542, 611, 642
BspMI ACCTGC 1 cut(s) 788
BspPI GGATC 2 cut(s) 535, 548
BspT107I GGYRCC 1 cut(s) 640
BsrDI GCAATG 1 cut(s) 157
BssECI CCNNGG 2 cut(s) 47, 694
BssMI GATC 1 cut(s) 540
BssT1I CCWWGG 1 cut(s) 694
Bst2UI CCWGG 2 cut(s) 49, 249
Bst4CI ACNGT 3 cut(s) 16, 42, 311
BstC8I GCNNGC 1 cut(s) 429
BstDEI CTNAG 5 cut(s) 375, 437, 494, 626, 679
BstF5I GGATG 1 cut(s) 523
BstHHI GCGC 1 cut(s) 194
BstKTI GATC 1 cut(s) 543
BstMAI GTCTC 1 cut(s) 764
BstMBI GATC 1 cut(s) 540
BstMWI GCNNNNNNNGC 1 cut(s) 433
BstNI CCWGG 2 cut(s) 49, 249
BstNSI RCATGY 1 cut(s) 409
BstSCI CCNGG 2 cut(s) 47, 247
BstV2I GAAGAC 2 cut(s) 136, 459
BstX2I RGATCY 1 cut(s) 540
BstYI RGATCY 1 cut(s) 540
BsuRI GGCC 2 cut(s) 5, 431
BtsCI GGATG 1 cut(s) 523
BtsIMutI CAGTG 1 cut(s) 21
BveI ACCTGC 1 cut(s) 788
Cac8I GCNNGC 1 cut(s) 429
CfoI GCGC 1 cut(s) 194
Cfr13I GGNCC 1 cut(s) 429
Csp6I GTAC 4 cut(s) 107, 590, 641, 733
CspCI CAANNNNNGTGG 2 cut(s) 73, 108
CviAII CATG 3 cut(s) 80, 406, 632
CviJI RGCY 9 cut(s) 5, 90, 272, 335, 431, 491, 581, 630, 703
CviKI_1 RGCY 9 cut(s) 5, 90, 272, 335, 431, 491, 581, 630, 703
CviQI GTAC 4 cut(s) 107, 590, 641, 733
DdeI CTNAG 5 cut(s) 375, 437, 494, 626, 679
DpnI GATC 1 cut(s) 542
DpnII GATC 1 cut(s) 540
Eco130I CCWWGG 1 cut(s) 694
Eco57I CTGAAG 2 cut(s) 130, 597
EcoRII CCWGG 2 cut(s) 47, 247
EcoT14I CCWWGG 1 cut(s) 694
ErhI CCWWGG 1 cut(s) 694
FaeI CATG 3 cut(s) 83, 409, 635
FalI AAGNNNNNCTT 2 cut(s) 416, 448
FatI CATG 3 cut(s) 79, 405, 631
FauI CCCGC 1 cut(s) 420
FokI GGATG 1 cut(s) 530
FspBI CTAG 4 cut(s) 305, 695, 704, 827
GlaI GCGC 1 cut(s) 193
HaeIII GGCC 2 cut(s) 5, 431
HapII CCGG 1 cut(s) 544
HhaI GCGC 1 cut(s) 194
Hin1II CATG 3 cut(s) 83, 409, 635
Hin6I GCGC 1 cut(s) 192
HinP1I GCGC 1 cut(s) 192
HindIII AAGCTT 2 cut(s) 88, 579
HinfI GANTC 4 cut(s) 96, 238, 722, 847
HpaII CCGG 1 cut(s) 544
HphI GGTGA 2 cut(s) 36, 296
Hpy166II GTNNAC 1 cut(s) 38
Hpy188I TCNGA 3 cut(s) 299, 503, 577
Hpy188III TCNNGA 6 cut(s) 263, 350, 365, 544, 805, 855
Hpy8I GTNNAC 1 cut(s) 38
Hpy99I CGWCG 1 cut(s) 756
HpyAV CCTTC 4 cut(s) 208, 473, 600, 802
HpyCH4III ACNGT 3 cut(s) 16, 42, 311
HpyCH4IV ACGT 1 cut(s) 328
HpyCH4V TGCA 7 cut(s) 62, 73, 485, 533, 710, 791, 797
HpyF10VI GCNNNNNNNGC 1 cut(s) 433
HpyF3I CTNAG 5 cut(s) 375, 437, 494, 626, 679
HpySE526I ACGT 1 cut(s) 328
Hsp92II CATG 3 cut(s) 83, 409, 635
HspAI GCGC 1 cut(s) 192
Kpn2I TCCGGA 1 cut(s) 543
KpnI GGTACC 1 cut(s) 644
Kzo9I GATC 1 cut(s) 540
LweI GCATC 4 cut(s) 71, 330, 472, 784
MaeI CTAG 4 cut(s) 305, 695, 704, 827
MaeII ACGT 1 cut(s) 328
MaeIII GTNAC 5 cut(s) 16, 42, 389, 521, 550
MalI GATC 1 cut(s) 542
MboI GATC 1 cut(s) 540
MboII GAAGA 5 cut(s) 125, 136, 365, 380, 464
MfeI CAATTG 1 cut(s) 528
MflI RGATCY 1 cut(s) 540
MluCI AATT 3 cut(s) 233, 528, 864
MmeI TCCRAC 1 cut(s) 811
MnlI CCTC 6 cut(s) 15, 434, 469, 596, 631, 827
MroI TCCGGA 1 cut(s) 543
MslI CAYNNNNRTG 2 cut(s) 78, 264
MspI CCGG 1 cut(s) 544
MspR9I CCNGG 2 cut(s) 49, 249
MunI CAATTG 1 cut(s) 528
Mva1269I GAATGC 1 cut(s) 73
MvaI CCWGG 2 cut(s) 49, 249
MwoI GCNNNNNNNGC 1 cut(s) 433
NdeII GATC 1 cut(s) 540
NlaIII CATG 3 cut(s) 83, 409, 635
NlaIV GGNNCC 3 cut(s) 542, 611, 642
NmuCI GTSAC 4 cut(s) 16, 42, 389, 550
NspI RCATGY 1 cut(s) 409
PciI ACATGT 1 cut(s) 405
PctI GAATGC 1 cut(s) 73
PfeI GAWTC 4 cut(s) 96, 238, 722, 847
PfoI TCCNGGA 1 cut(s) 247
PscI ACATGT 1 cut(s) 405
Psp6I CCWGG 2 cut(s) 47, 247
PspGI CCWGG 2 cut(s) 47, 247
PspN4I GGNNCC 3 cut(s) 542, 611, 642
PspPI GGNCC 1 cut(s) 429
PsuI RGATCY 1 cut(s) 540
RsaI GTAC 4 cut(s) 108, 591, 642, 734
RsaNI GTAC 4 cut(s) 107, 590, 641, 733
RseI CAYNNNNRTG 2 cut(s) 78, 264
Sau3AI GATC 1 cut(s) 540
Sau96I GGNCC 1 cut(s) 429
ScaI AGTACT 1 cut(s) 734
ScrFI CCNGG 2 cut(s) 49, 249
SfaNI GCATC 4 cut(s) 71, 330, 472, 784
SmiMI CAYNNNNRTG 2 cut(s) 78, 264
SmlI CTYRAG 1 cut(s) 7
SmoI CTYRAG 1 cut(s) 7
Sse9I AATT 3 cut(s) 233, 528, 864
SsiI CCGC 1 cut(s) 427
SspMI CTAG 4 cut(s) 305, 695, 704, 827
StyD4I CCNGG 2 cut(s) 47, 247
StyI CCWWGG 1 cut(s) 694
TaaI ACNGT 3 cut(s) 16, 42, 311
TaiI ACGT 1 cut(s) 331
TasI AATT 3 cut(s) 233, 528, 864
TatI WGTACW 1 cut(s) 732
TfiI GAWTC 4 cut(s) 96, 238, 722, 847
TscAI CASTG 1 cut(s) 21
TseFI GTSAC 4 cut(s) 16, 42, 389, 550
Tsp45I GTSAC 4 cut(s) 16, 42, 389, 550
TspDTI ATGAA 6 cut(s) 132, 191, 269, 663, 834, 839
TspRI CASTG 1 cut(s) 21
XapI RAATTY 1 cut(s) 864
XceI RCATGY 1 cut(s) 409
XmaJI CCTAGG 1 cut(s) 694
XspI CTAG 4 cut(s) 305, 695, 704, 827
ZrmI AGTACT 1 cut(s) 734
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.