RchiOBHm_Chr4g0400651

Methyltransferase required for the conversion of 2- polyprenyl-6-methoxy-1,4-benzoquinol (DDMQH2) to 2-polyprenyl-3- methyl-6-methoxy-1,4-benzoquinol (DMQH2)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
4
OG0002791
Physical Location & Seq
Reverse (-)
18384085 .. 18387075
2991 bp
Loading structure...
UTR
Exon/CDS
Intron
PRQ37264

Sequence Viewer

Length: 357 bp
ATGGATGGTTACACAATTGCATTTGGGATCCGGAATGTCACACACATAGAGAAAGCACTTTCTGAAGCTTATAGGGTACTAAAACGGGGAGGAAGGTTCCTGTGCCTCGAACTCAGCCATGTAGAGGTACCTGTATTCAAAGGCTTCTATGACTTTTATTCATTCTCAATCATTCCCACCCTAGGAGAGCTAGTTGCAGGGGATAGAGAATCTTATCAGTACTTGGTAGAGAGTATTCGTCGTTTTCCGCCACAGGAGACATTTGCGTCAATGATTGCAGACGCAGGTTTCCAGAAGGTTGAGTATGAAAATCTAGTTGGAGGAGTGGTAGCGATTCATTCAGGGTTGAAAATTTAG

Protein Analysis

118

Amino Acids

13.29

Weight (kDa)

5.89

Isoelectric Point (pI)

39.28

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Ubie_methyltran PF01209 2 - 117 8.9e-48 ubiE/COQ5 methyltransferase family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 265
Acc36I ACCTGC 1 cut(s) 275
Acc65I GGTACC 1 cut(s) 127
AccB1I GGYRCC 1 cut(s) 127
AccIII TCCGGA 1 cut(s) 30
AciI CCGC 1 cut(s) 248
AclWI GGATC 2 cut(s) 22, 35
AcsI RAATTY 1 cut(s) 351
AcuI CTGAAG 1 cut(s) 84
AfaI GTAC 3 cut(s) 78, 129, 221
AfiI CCNNNNNNNGG 2 cut(s) 124, 182
AgsI TTSAA 2 cut(s) 139, 349
AjuI GAANNNNNNNTTGG 2 cut(s) 300, 332
AluBI AGCT 2 cut(s) 68, 190
AluI AGCT 2 cut(s) 68, 190
Alw26I GTCTC 1 cut(s) 251
AlwI GGATC 2 cut(s) 22, 35
Aor13HI TCCGGA 1 cut(s) 30
ApoI RAATTY 1 cut(s) 351
Asp718I GGTACC 1 cut(s) 127
AspA2I CCTAGG 1 cut(s) 181
AvrII CCTAGG 1 cut(s) 181
BamHI GGATCC 1 cut(s) 27
BanI GGYRCC 1 cut(s) 127
BcoDI GTCTC 1 cut(s) 251
BfaI CTAG 3 cut(s) 182, 191, 314
BfuAI ACCTGC 1 cut(s) 275
BlnI CCTAGG 1 cut(s) 181
BmcAI AGTACT 1 cut(s) 221
BmiI GGNNCC 3 cut(s) 29, 98, 129
BsaJI CCNNGG 1 cut(s) 181
BsaWI WCCGGW 1 cut(s) 30
BsaXI ACNNNNNCTCC 4 cut(s) 177, 207, 312, 342
Bsc4I CCNNNNNNNGG 2 cut(s) 124, 182
BseAI TCCGGA 1 cut(s) 30
BseDI CCNNGG 1 cut(s) 181
BseGI GGATG 1 cut(s) 10
BseLI CCNNNNNNNGG 2 cut(s) 124, 182
BseMII CTCAG 1 cut(s) 127
BseRI GAGGAG 1 cut(s) 336
BshNI GGYRCC 1 cut(s) 127
BsiSI CCGG 1 cut(s) 31
BslI CCNNNNNNNGG 2 cut(s) 124, 182
BsmAI GTCTC 1 cut(s) 251
Bsp13I TCCGGA 1 cut(s) 30
Bsp143I GATC 1 cut(s) 27
BspACI CCGC 1 cut(s) 248
BspCNI CTCAG 1 cut(s) 126
BspEI TCCGGA 1 cut(s) 30
BspLI GGNNCC 3 cut(s) 29, 98, 129
BspMI ACCTGC 1 cut(s) 275
BspPI GGATC 2 cut(s) 22, 35
BspT107I GGYRCC 1 cut(s) 127
BssECI CCNNGG 1 cut(s) 181
BssMI GATC 1 cut(s) 27
BssT1I CCWWGG 1 cut(s) 181
BstDEI CTNAG 1 cut(s) 113
BstF5I GGATG 1 cut(s) 10
BstKTI GATC 1 cut(s) 30
BstMAI GTCTC 1 cut(s) 251
BstMBI GATC 1 cut(s) 27
BstX2I RGATCY 1 cut(s) 27
BstYI RGATCY 1 cut(s) 27
BtsCI GGATG 1 cut(s) 10
BveI ACCTGC 1 cut(s) 275
CseI GACGC 2 cut(s) 255, 290
Csp6I GTAC 3 cut(s) 77, 128, 220
CviAII CATG 1 cut(s) 119
CviJI RGCY 4 cut(s) 68, 117, 144, 190
CviKI_1 RGCY 4 cut(s) 68, 117, 144, 190
CviQI GTAC 3 cut(s) 77, 128, 220
DdeI CTNAG 1 cut(s) 113
DpnI GATC 1 cut(s) 29
DpnII GATC 1 cut(s) 27
DrdI GACNNNNNNGTC 1 cut(s) 265
DseDI GACNNNNNNGTC 1 cut(s) 265
EciI GGCGGA 1 cut(s) 237
Eco130I CCWWGG 1 cut(s) 181
Eco57I CTGAAG 1 cut(s) 84
EcoT14I CCWWGG 1 cut(s) 181
ErhI CCWWGG 1 cut(s) 181
FaeI CATG 1 cut(s) 122
FaiI YATR 5 cut(s) 47, 72, 120, 150, 306
FatI CATG 1 cut(s) 118
FokI GGATG 1 cut(s) 17
FspBI CTAG 3 cut(s) 182, 191, 314
HapII CCGG 1 cut(s) 31
HgaI GACGC 2 cut(s) 255, 290
Hin1II CATG 1 cut(s) 122
HindIII AAGCTT 1 cut(s) 66
HinfI GANTC 2 cut(s) 209, 334
HpaII CCGG 1 cut(s) 31
Hpy188I TCNGA 1 cut(s) 64
Hpy188III TCNNGA 2 cut(s) 31, 292
Hpy99I CGWCG 1 cut(s) 243
HpyAV CCTTC 2 cut(s) 87, 289
HpyCH4V TGCA 3 cut(s) 20, 197, 278
HpyF3I CTNAG 1 cut(s) 113
Hsp92II CATG 1 cut(s) 122
Kpn2I TCCGGA 1 cut(s) 30
KpnI GGTACC 1 cut(s) 131
Kzo9I GATC 1 cut(s) 27
LpnPI CCDG 8 cut(s) 44, 113, 144, 183, 239, 270, 305, 327
MaeI CTAG 3 cut(s) 182, 191, 314
MaeIII GTNAC 2 cut(s) 8, 37
MalI GATC 1 cut(s) 29
MboI GATC 1 cut(s) 27
MfeI CAATTG 1 cut(s) 15
MflI RGATCY 1 cut(s) 27
MluCI AATT 2 cut(s) 15, 351
MmeI TCCRAC 1 cut(s) 298
MnlI CCTC 4 cut(s) 83, 116, 118, 314
MroI TCCGGA 1 cut(s) 30
MspI CCGG 1 cut(s) 31
MunI CAATTG 1 cut(s) 15
NdeII GATC 1 cut(s) 27
NlaIII CATG 1 cut(s) 122
NlaIV GGNNCC 3 cut(s) 29, 98, 129
NmuCI GTSAC 1 cut(s) 37
PfeI GAWTC 2 cut(s) 209, 334
PspN4I GGNNCC 3 cut(s) 29, 98, 129
PsuI RGATCY 1 cut(s) 27
RsaI GTAC 3 cut(s) 78, 129, 221
RsaNI GTAC 3 cut(s) 77, 128, 220
Sau3AI GATC 1 cut(s) 27
ScaI AGTACT 1 cut(s) 221
SetI ASST 7 cut(s) 70, 98, 129, 133, 192, 289, 300
Sse9I AATT 2 cut(s) 15, 351
SsiI CCGC 1 cut(s) 248
SspMI CTAG 3 cut(s) 182, 191, 314
StyI CCWWGG 1 cut(s) 181
TaqI TCGA 1 cut(s) 108
TasI AATT 2 cut(s) 15, 351
TatI WGTACW 1 cut(s) 219
TfiI GAWTC 2 cut(s) 209, 334
TseFI GTSAC 1 cut(s) 37
Tsp45I GTSAC 1 cut(s) 37
TspDTI ATGAA 3 cut(s) 150, 321, 326
XapI RAATTY 1 cut(s) 351
XmaJI CCTAGG 1 cut(s) 181
XspI CTAG 3 cut(s) 182, 191, 314
ZrmI AGTACT 1 cut(s) 221
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.