Rh7BG256500

Methyltransferase required for the conversion of 2- polyprenyl-6-methoxy-1,4-benzoquinol (DDMQH2) to 2-polyprenyl-3- methyl-6-methoxy-1,4-benzoquinol (DMQH2)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr7B
OG0002791
Physical Location & Seq
Reverse (-)
23329999 .. 23336577
6579 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh7BG256500.1

Sequence Viewer

Length: 663 bp
ATGGCCTTGAGAACAGTGACGAGGAAGATAGGGAGTAAACTGTTACCCAGGTTTTGTCCTGCATCTCTATTGCATTCTCATGCCACAAGCTTTGGATTCAAGGAAGTACGGGAAGATGAAAAAAGTCAAATGGTTGGTAATGTCTTCAGCAATGTTGCTTCAAACTATGATACAATGAATGATTTGATGAGTGCAGGATTACACAGATTATGGAAGGATAGATTGGTTTCAAAACTGAATCCTTTCCCTGGAATGAACCATCTTGATGTGGCTGGTGGGACAGGTGATGTTGCTTTCAGAATACTAGACAGTATAAACAGCATCAAACGTAGAGCTTTGCTGAATGTTCTTGAAGATGATTTACAGGAAGAAACTAAGGTGTATGTATGTGACATCAATCCCAACATGTTGAGTGTTGGTAAAAAGCGGGCCTTGCAGAGAGGTCTTGGAGAAGACAAATCCCTTCTATGGGTGGAGGGAGATGCAGAAGCCTTGAGTTTCGGAGATAATACAATGGATGGTTACACAATTGCATTTGGGATCCGGAATGTCACACACATAGAGAAAGCACTTTCTGAAGCTTATAGGGTACTAAAACGGGGAGGAAGGTTCCTGTGCCTTGAACTCAGCCATGTAGAGGTACCTGTATTCAAAGACTTGTAA

Protein Analysis

220

Amino Acids

24.57

Weight (kDa)

7.75

Isoelectric Point (pI)

35.73

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Ubie_methyltran PF01209 39 - 218 3.9e-53 ubiE/COQ5 methyltransferase family
Methyltransf_31 PF13847 83 - 210 2.6e-07 Methyltransferase domain
Methyltransf_12 PF08242 88 - 204 1.9e-07 Methyltransferase domain
Methyltransf_25 PF13649 121 - 202 7.5e-12 Methyltransferase domain
Methyltransf_11 PF08241 125 - 206 4.1e-14 Methyltransferase domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc65I GGTACC 1 cut(s) 640
AccB1I GGYRCC 1 cut(s) 640
AccIII TCCGGA 1 cut(s) 543
AciI CCGC 1 cut(s) 427
AclWI GGATC 2 cut(s) 535, 548
AcuI CTGAAG 2 cut(s) 130, 597
AfaI GTAC 3 cut(s) 108, 591, 642
AfiI CCNNNNNNNGG 4 cut(s) 248, 468, 469, 637
AflIII ACRYGT 1 cut(s) 405
AgsI TTSAA 6 cut(s) 100, 162, 231, 353, 623, 652
AjnI CCWGG 2 cut(s) 47, 247
AjuI GAANNNNNNNTTGG 2 cut(s) 206, 238
AluBI AGCT 3 cut(s) 90, 335, 581
AluI AGCT 3 cut(s) 90, 335, 581
AlwI GGATC 2 cut(s) 535, 548
Aor13HI TCCGGA 1 cut(s) 543
AoxI GGCC 2 cut(s) 3, 429
Asp700I GAANNNNTTC 1 cut(s) 242
Asp718I GGTACC 1 cut(s) 640
AspS9I GGNCC 1 cut(s) 429
AsuHPI GGTGA 1 cut(s) 296
BamHI GGATCC 1 cut(s) 540
BanI GGYRCC 1 cut(s) 640
BarI GAAGNNNNNNTAC 2 cut(s) 345, 377
BbsI GAAGAC 2 cut(s) 136, 459
BccI CCATC 2 cut(s) 267, 512
BciT130I CCWGG 2 cut(s) 49, 249
BfaI CTAG 1 cut(s) 305
Bme1390I CCNGG 2 cut(s) 49, 249
BmgT120I GGNCC 1 cut(s) 429
BmiI GGNNCC 3 cut(s) 542, 611, 642
BmrFI CCNGG 2 cut(s) 49, 249
BmsI GCATC 3 cut(s) 71, 330, 472
BpiI GAAGAC 2 cut(s) 136, 459
BpuEI CTTGAG 2 cut(s) 28, 514
BsaJI CCNNGG 2 cut(s) 47, 247
BsaWI WCCGGW 1 cut(s) 543
Bsc4I CCNNNNNNNGG 4 cut(s) 248, 468, 469, 637
Bse3DI GCAATG 1 cut(s) 157
BseAI TCCGGA 1 cut(s) 543
BseBI CCWGG 2 cut(s) 49, 249
BseDI CCNNGG 2 cut(s) 47, 247
BseGI GGATG 1 cut(s) 523
BseLI CCNNNNNNNGG 4 cut(s) 248, 468, 469, 637
BseMI GCAATG 1 cut(s) 157
BseMII CTCAG 1 cut(s) 640
BsgI GTGCAG 1 cut(s) 213
BshFI GGCC 2 cut(s) 5, 431
BshNI GGYRCC 1 cut(s) 640
BsiSI CCGG 1 cut(s) 544
BslFI GGGAC 1 cut(s) 292
BslI CCNNNNNNNGG 4 cut(s) 248, 468, 469, 637
BsmFI GGGAC 1 cut(s) 292
BsmI GAATGC 1 cut(s) 73
BsnI GGCC 2 cut(s) 5, 431
Bsp13I TCCGGA 1 cut(s) 543
Bsp143I GATC 1 cut(s) 540
BspACI CCGC 1 cut(s) 427
BspANI GGCC 2 cut(s) 5, 431
BspCNI CTCAG 1 cut(s) 639
BspEI TCCGGA 1 cut(s) 543
BspLI GGNNCC 3 cut(s) 542, 611, 642
BspPI GGATC 2 cut(s) 535, 548
BspT107I GGYRCC 1 cut(s) 640
BsrDI GCAATG 1 cut(s) 157
BssECI CCNNGG 2 cut(s) 47, 247
BssMI GATC 1 cut(s) 540
Bst2UI CCWGG 2 cut(s) 49, 249
Bst4CI ACNGT 3 cut(s) 16, 42, 311
BstC8I GCNNGC 1 cut(s) 429
BstDEI CTNAG 2 cut(s) 375, 626
BstF5I GGATG 1 cut(s) 523
BstKTI GATC 1 cut(s) 543
BstMBI GATC 1 cut(s) 540
BstMWI GCNNNNNNNGC 1 cut(s) 433
BstNI CCWGG 2 cut(s) 49, 249
BstNSI RCATGY 1 cut(s) 409
BstSCI CCNGG 2 cut(s) 47, 247
BstV2I GAAGAC 2 cut(s) 136, 459
BstX2I RGATCY 1 cut(s) 540
BstYI RGATCY 1 cut(s) 540
BsuRI GGCC 2 cut(s) 5, 431
BtsCI GGATG 1 cut(s) 523
BtsIMutI CAGTG 1 cut(s) 21
Cac8I GCNNGC 1 cut(s) 429
Cfr13I GGNCC 1 cut(s) 429
Csp6I GTAC 3 cut(s) 107, 590, 641
CspCI CAANNNNNGTGG 2 cut(s) 73, 108
CviAII CATG 3 cut(s) 80, 406, 632
CviJI RGCY 8 cut(s) 5, 90, 272, 335, 431, 491, 581, 630
CviKI_1 RGCY 8 cut(s) 5, 90, 272, 335, 431, 491, 581, 630
CviQI GTAC 3 cut(s) 107, 590, 641
DdeI CTNAG 2 cut(s) 375, 626
DpnI GATC 1 cut(s) 542
DpnII GATC 1 cut(s) 540
Eco57I CTGAAG 2 cut(s) 130, 597
EcoRII CCWGG 2 cut(s) 47, 247
FaeI CATG 3 cut(s) 83, 409, 635
FalI AAGNNNNNCTT 2 cut(s) 416, 448
FaqI GGGAC 1 cut(s) 292
FatI CATG 3 cut(s) 79, 405, 631
FauI CCCGC 1 cut(s) 420
FokI GGATG 1 cut(s) 530
FspBI CTAG 1 cut(s) 305
HaeIII GGCC 2 cut(s) 5, 431
HapII CCGG 1 cut(s) 544
Hin1II CATG 3 cut(s) 83, 409, 635
HindIII AAGCTT 2 cut(s) 88, 579
HinfI GANTC 2 cut(s) 96, 238
HpaII CCGG 1 cut(s) 544
HphI GGTGA 1 cut(s) 296
Hpy166II GTNNAC 1 cut(s) 38
Hpy188I TCNGA 3 cut(s) 299, 503, 577
Hpy188III TCNNGA 3 cut(s) 263, 350, 544
Hpy8I GTNNAC 1 cut(s) 38
HpyAV CCTTC 3 cut(s) 208, 473, 600
HpyCH4III ACNGT 3 cut(s) 16, 42, 311
HpyCH4IV ACGT 1 cut(s) 328
HpyCH4V TGCA 6 cut(s) 62, 73, 194, 436, 485, 533
HpyF10VI GCNNNNNNNGC 1 cut(s) 433
HpyF3I CTNAG 2 cut(s) 375, 626
HpySE526I ACGT 1 cut(s) 328
Hsp92II CATG 3 cut(s) 83, 409, 635
Kpn2I TCCGGA 1 cut(s) 543
KpnI GGTACC 1 cut(s) 644
Kzo9I GATC 1 cut(s) 540
LweI GCATC 3 cut(s) 71, 330, 472
MaeI CTAG 1 cut(s) 305
MaeII ACGT 1 cut(s) 328
MaeIII GTNAC 5 cut(s) 16, 42, 389, 521, 550
MalI GATC 1 cut(s) 542
MboI GATC 1 cut(s) 540
MboII GAAGA 6 cut(s) 37, 125, 136, 365, 380, 464
MfeI CAATTG 1 cut(s) 528
MflI RGATCY 1 cut(s) 540
MluCI AATT 1 cut(s) 528
MnlI CCTC 5 cut(s) 15, 434, 469, 596, 631
MroI TCCGGA 1 cut(s) 543
MroXI GAANNNNTTC 1 cut(s) 242
MslI CAYNNNNRTG 2 cut(s) 78, 264
MspI CCGG 1 cut(s) 544
MspR9I CCNGG 2 cut(s) 49, 249
MunI CAATTG 1 cut(s) 528
Mva1269I GAATGC 1 cut(s) 73
MvaI CCWGG 2 cut(s) 49, 249
MwoI GCNNNNNNNGC 1 cut(s) 433
NdeII GATC 1 cut(s) 540
NlaIII CATG 3 cut(s) 83, 409, 635
NlaIV GGNNCC 3 cut(s) 542, 611, 642
NmuCI GTSAC 3 cut(s) 16, 389, 550
NspI RCATGY 1 cut(s) 409
PciI ACATGT 1 cut(s) 405
PctI GAATGC 1 cut(s) 73
PdmI GAANNNNTTC 1 cut(s) 242
PfeI GAWTC 2 cut(s) 96, 238
PscI ACATGT 1 cut(s) 405
Psp6I CCWGG 2 cut(s) 47, 247
PspGI CCWGG 2 cut(s) 47, 247
PspN4I GGNNCC 3 cut(s) 542, 611, 642
PspPI GGNCC 1 cut(s) 429
PsuI RGATCY 1 cut(s) 540
RsaI GTAC 3 cut(s) 108, 591, 642
RsaNI GTAC 3 cut(s) 107, 590, 641
RseI CAYNNNNRTG 2 cut(s) 78, 264
Sau3AI GATC 1 cut(s) 540
Sau96I GGNCC 1 cut(s) 429
ScrFI CCNGG 2 cut(s) 49, 249
SfaNI GCATC 3 cut(s) 71, 330, 472
SmiMI CAYNNNNRTG 2 cut(s) 78, 264
SmlI CTYRAG 2 cut(s) 7, 493
SmoI CTYRAG 2 cut(s) 7, 493
Sse9I AATT 1 cut(s) 528
SsiI CCGC 1 cut(s) 427
SspMI CTAG 1 cut(s) 305
StyD4I CCNGG 2 cut(s) 47, 247
TaaI ACNGT 3 cut(s) 16, 42, 311
TaiI ACGT 1 cut(s) 331
TasI AATT 1 cut(s) 528
TfiI GAWTC 2 cut(s) 96, 238
TscAI CASTG 1 cut(s) 21
TseFI GTSAC 3 cut(s) 16, 389, 550
Tsp45I GTSAC 3 cut(s) 16, 389, 550
TspDTI ATGAA 3 cut(s) 132, 191, 269
TspRI CASTG 1 cut(s) 21
XceI RCATGY 1 cut(s) 409
XmnI GAANNNNTTC 1 cut(s) 242
XspI CTAG 1 cut(s) 305
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.