Rh4AG101300

Methyltransferase required for the conversion of 2- polyprenyl-6-methoxy-1,4-benzoquinol (DDMQH2) to 2-polyprenyl-3- methyl-6-methoxy-1,4-benzoquinol (DMQH2)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr4A
OG0002791
Physical Location & Seq
Reverse (-)
21514146 .. 21515582
1437 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh4AG101300.1

Sequence Viewer

Length: 342 bp
ATGGTCTTTTTTTTTTTTTTTTTTTTTTACAGGTATACAGTTATGGCCTTGAGAACAGTGACGAGGAAGTTAGGGAGTAGACTGTTACCCAGGTTGTCTCCTGCATCTCTATTTCATTCTCATGCCACAAGCTTTGGATTCAAGGAAGTACGGGAAGATGAAAAAAGTCAAATGGTTGGTAATGTCTTCAGCAATGTTGCTTCAAACTATGATACAATGAATGATTTGATGAGTGCGGGATTACAAAGATTATGGAAGGATAGATTGGTTTCAAAACTGAATCCTTTTCCTGGAATGAACCATCTTGATGTGGCTGGTGGGACAGGTGATGTTGCTTTCTGA

Protein Analysis

113

Amino Acids

12.88

Weight (kDa)

9.77

Isoelectric Point (pI)

31.05

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Ubie_methyltran PF01209 53 - 113 2e-16 ubiE/COQ5 methyltransferase family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccI GTMKAC 2 cut(s) 35, 79
AciI CCGC 1 cut(s) 236
AcuI CTGAAG 1 cut(s) 172
AfaI GTAC 1 cut(s) 150
AfiI CCNNNNNNNGG 1 cut(s) 290
AgsI TTSAA 3 cut(s) 142, 204, 273
AjnI CCWGG 2 cut(s) 89, 289
AjuI GAANNNNNNNTTGG 2 cut(s) 248, 280
AluBI AGCT 1 cut(s) 132
AluI AGCT 1 cut(s) 132
Alw26I GTCTC 1 cut(s) 102
AoxI GGCC 1 cut(s) 45
AsuHPI GGTGA 1 cut(s) 338
BbsI GAAGAC 1 cut(s) 178
BccI CCATC 1 cut(s) 309
BciT130I CCWGG 2 cut(s) 91, 291
BcoDI GTCTC 1 cut(s) 102
Bme1390I CCNGG 2 cut(s) 91, 291
BmrFI CCNGG 2 cut(s) 91, 291
BmsI GCATC 1 cut(s) 113
BpiI GAAGAC 1 cut(s) 178
BpuEI CTTGAG 1 cut(s) 70
BsaJI CCNNGG 1 cut(s) 89
Bsc4I CCNNNNNNNGG 1 cut(s) 290
Bse3DI GCAATG 1 cut(s) 199
BseBI CCWGG 2 cut(s) 91, 291
BseDI CCNNGG 1 cut(s) 89
BseLI CCNNNNNNNGG 1 cut(s) 290
BseMI GCAATG 1 cut(s) 199
BshFI GGCC 1 cut(s) 47
BslFI GGGAC 1 cut(s) 334
BslI CCNNNNNNNGG 1 cut(s) 290
BsmAI GTCTC 1 cut(s) 102
BsmFI GGGAC 1 cut(s) 334
BsnI GGCC 1 cut(s) 47
BspACI CCGC 1 cut(s) 236
BspANI GGCC 1 cut(s) 47
BsrDI GCAATG 1 cut(s) 199
BssECI CCNNGG 1 cut(s) 89
BssNAI GTATAC 1 cut(s) 36
Bst1107I GTATAC 1 cut(s) 36
Bst2UI CCWGG 2 cut(s) 91, 291
Bst4CI ACNGT 3 cut(s) 40, 58, 84
BstMAI GTCTC 1 cut(s) 102
BstNI CCWGG 2 cut(s) 91, 291
BstSCI CCNGG 2 cut(s) 89, 289
BstV2I GAAGAC 1 cut(s) 178
BstZ17I GTATAC 1 cut(s) 36
BsuRI GGCC 1 cut(s) 47
BtsIMutI CAGTG 1 cut(s) 63
Csp6I GTAC 1 cut(s) 149
CspCI CAANNNNNGTGG 2 cut(s) 115, 150
CviAII CATG 1 cut(s) 122
CviJI RGCY 3 cut(s) 47, 132, 314
CviKI_1 RGCY 3 cut(s) 47, 132, 314
CviQI GTAC 1 cut(s) 149
Eco57I CTGAAG 1 cut(s) 172
EcoRII CCWGG 2 cut(s) 89, 289
FaeI CATG 1 cut(s) 125
FaiI YATR 5 cut(s) 36, 44, 123, 210, 253
FaqI GGGAC 1 cut(s) 334
FatI CATG 1 cut(s) 121
FauI CCCGC 1 cut(s) 229
FblI GTMKAC 2 cut(s) 35, 79
HaeIII GGCC 1 cut(s) 47
Hin1II CATG 1 cut(s) 125
HindIII AAGCTT 1 cut(s) 130
HinfI GANTC 2 cut(s) 138, 280
HphI GGTGA 1 cut(s) 338
Hpy166II GTNNAC 2 cut(s) 36, 80
Hpy188I TCNGA 1 cut(s) 341
Hpy188III TCNNGA 1 cut(s) 305
Hpy8I GTNNAC 2 cut(s) 36, 80
HpyAV CCTTC 1 cut(s) 250
HpyCH4III ACNGT 3 cut(s) 40, 58, 84
HpyCH4V TGCA 1 cut(s) 104
Hsp92II CATG 1 cut(s) 125
LpnPI CCDG 8 cut(s) 16, 76, 103, 114, 276, 300, 303, 309
LweI GCATC 1 cut(s) 113
MaeIII GTNAC 2 cut(s) 58, 84
MboII GAAGA 2 cut(s) 167, 178
MnlI CCTC 1 cut(s) 57
MslI CAYNNNNRTG 2 cut(s) 120, 306
MspR9I CCNGG 2 cut(s) 91, 291
MvaI CCWGG 2 cut(s) 91, 291
NlaIII CATG 1 cut(s) 125
NmuCI GTSAC 1 cut(s) 58
PfeI GAWTC 2 cut(s) 138, 280
PfoI TCCNGGA 1 cut(s) 289
Psp6I CCWGG 2 cut(s) 89, 289
PspGI CCWGG 2 cut(s) 89, 289
RsaI GTAC 1 cut(s) 150
RsaNI GTAC 1 cut(s) 149
RseI CAYNNNNRTG 2 cut(s) 120, 306
ScrFI CCNGG 2 cut(s) 91, 291
SetI ASST 4 cut(s) 35, 95, 134, 328
SfaNI GCATC 1 cut(s) 113
SmiMI CAYNNNNRTG 2 cut(s) 120, 306
SmlI CTYRAG 1 cut(s) 49
SmoI CTYRAG 1 cut(s) 49
SsiI CCGC 1 cut(s) 236
StyD4I CCNGG 2 cut(s) 89, 289
TaaI ACNGT 3 cut(s) 40, 58, 84
TfiI GAWTC 2 cut(s) 138, 280
TscAI CASTG 1 cut(s) 63
TseFI GTSAC 1 cut(s) 58
Tsp45I GTSAC 1 cut(s) 58
TspDTI ATGAA 4 cut(s) 104, 174, 233, 311
TspRI CASTG 1 cut(s) 63
XmiI GTMKAC 2 cut(s) 35, 79
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.