RchiOBHm_Chr4g0400411

Methyltransferase required for the conversion of 2- polyprenyl-6-methoxy-1,4-benzoquinol (DDMQH2) to 2-polyprenyl-3- methyl-6-methoxy-1,4-benzoquinol (DMQH2)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
4
OG0002791
Physical Location & Seq
Reverse (-)
17989753 .. 17994700
4948 bp
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UTR
Exon/CDS
Intron
PRQ37245

Sequence Viewer

Length: 912 bp
ATGGTTTTGCACAGGTTTACAGTTATGGCCTTGAGAACAGTGAAGAGGAAGATAGGGAGTAAACTGTTACCCAGGTTTTGCCCTGCATCTCTATTGCATTCTCATGCCACAAGCTTTGGATTCAAGGAAGTACGGGAAGAGGAAAAAAGTCAAATGGTTGGTAATGTCTTCAGCAATGTTGCTTCAAACTATGATACAATGAACGATTTGATGAGTATTGGTTTCCAAACTGAATCTTTTCCTGGAATGAACCATCTTGATGTGGCTGGTGGAACAGGTGATGTTGCTTTCAGAATACTAGACAGTATAAACAGCATCAAACGTAGAGCTTTGCTGAATGTTCTTGAAGATGATTTACAGGAAGAAACTAAGGTGTATGTATGTGACATCAATCCCAACATGTTGAATGTTGGTAAAAAGCGAGCCTTGCAGAGAGGTCTTGGAGAAGATAAATCCCTTCTATGGGTGGAGGGAGATGCAGAAGTCCTGAGTTTCGGAGATAATACAATGGATGGTTACACAATTGCATTTGGGATCCGGAATGTCACACACATAGAGAAAGCACTTTCTGAAGCTTATAGGGTACTAAAACGGGGAGGAAGGTTCCTGTGCCTTGAACTCAGCCATGTAAATTTACCTGTATTCAAAGACTTAAGCTGTGTTTTGATATATATGGTTTGTCTATTTTTTTGGTCTTGCCGCTATGACTTTTATTCATTCTCAATCATTCCCACCCTAGGAGAGCTAGTTGCAGGAGATAGAGAATCTTATCAGTACTTGGTAGAGAGTATTCGTCGTTTTCCACCACAGGAGACATTTGCTTCAATGATTGCAGATGCAGGTTTCCAGAAGGTTGAGTATGAAAATCTAGTTGGAGGAGTGGTAGCGATTCATTCAGGGTTGAAAATTTAG

Protein Analysis

303

Amino Acids

34.1

Weight (kDa)

6.19

Isoelectric Point (pI)

41.72

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Ubie_methyltran PF01209 48 - 302 4.6e-68 ubiE/COQ5 methyltransferase family
Methyltransf_31 PF13847 81 - 208 1.7e-06 Methyltransferase domain
Methyltransf_12 PF08242 86 - 202 5.2e-07 Methyltransferase domain
Methyltransf_25 PF13649 119 - 200 5.4e-11 Methyltransferase domain
Methyltransf_11 PF08241 123 - 204 3e-13 Methyltransferase domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 830
AccIII TCCGGA 1 cut(s) 537
AciI CCGC 1 cut(s) 700
AclWI GGATC 2 cut(s) 529, 542
AcsI RAATTY 2 cut(s) 631, 906
AcuI CTGAAG 2 cut(s) 154, 591
AfaI GTAC 3 cut(s) 132, 585, 776
AfiI CCNNNNNNNGG 3 cut(s) 462, 463, 737
AflII CTTAAG 1 cut(s) 652
AflIII ACRYGT 1 cut(s) 399
AgsI TTSAA 8 cut(s) 124, 186, 347, 406, 617, 646, 825, 904
AjnI CCWGG 2 cut(s) 71, 241
AjuI GAANNNNNNNTTGG 2 cut(s) 855, 887
AluBI AGCT 5 cut(s) 114, 329, 575, 657, 745
AluI AGCT 5 cut(s) 114, 329, 575, 657, 745
Alw26I GTCTC 1 cut(s) 806
AlwI GGATC 2 cut(s) 529, 542
Aor13HI TCCGGA 1 cut(s) 537
AoxI GGCC 1 cut(s) 27
ApoI RAATTY 2 cut(s) 631, 906
Asp700I GAANNNNTTC 1 cut(s) 237
AspA2I CCTAGG 1 cut(s) 736
AsuHPI GGTGA 1 cut(s) 290
AvrII CCTAGG 1 cut(s) 736
BamHI GGATCC 1 cut(s) 534
BarI GAAGNNNNNNTAC 2 cut(s) 339, 371
BbsI GAAGAC 1 cut(s) 160
BccI CCATC 2 cut(s) 261, 506
BciT130I CCWGG 2 cut(s) 73, 243
BcoDI GTCTC 1 cut(s) 806
BfaI CTAG 4 cut(s) 299, 737, 746, 869
BfrI CTTAAG 1 cut(s) 652
BfuAI ACCTGC 1 cut(s) 830
BisI GCNGC 1 cut(s) 700
BlnI CCTAGG 1 cut(s) 736
BlsI GCNGC 1 cut(s) 701
BmcAI AGTACT 1 cut(s) 776
Bme1390I CCNGG 2 cut(s) 73, 243
BmiI GGNNCC 2 cut(s) 536, 605
BmrFI CCNGG 2 cut(s) 73, 243
BmsI GCATC 4 cut(s) 95, 324, 466, 826
BpiI GAAGAC 1 cut(s) 160
BpuEI CTTGAG 1 cut(s) 52
BsaJI CCNNGG 2 cut(s) 71, 736
BsaWI WCCGGW 1 cut(s) 537
BsaXI ACNNNNNCTCC 4 cut(s) 732, 762, 867, 897
Bsc4I CCNNNNNNNGG 3 cut(s) 462, 463, 737
Bse3DI GCAATG 1 cut(s) 181
BseAI TCCGGA 1 cut(s) 537
BseBI CCWGG 2 cut(s) 73, 243
BseDI CCNNGG 2 cut(s) 71, 736
BseGI GGATG 1 cut(s) 517
BseLI CCNNNNNNNGG 3 cut(s) 462, 463, 737
BseMI GCAATG 1 cut(s) 181
BseMII CTCAG 2 cut(s) 479, 634
BseRI GAGGAG 1 cut(s) 891
BshFI GGCC 1 cut(s) 29
BsiSI CCGG 1 cut(s) 538
BslI CCNNNNNNNGG 3 cut(s) 462, 463, 737
BsmAI GTCTC 1 cut(s) 806
BsmI GAATGC 1 cut(s) 97
BsnI GGCC 1 cut(s) 29
Bsp13I TCCGGA 1 cut(s) 537
Bsp143I GATC 1 cut(s) 534
BspACI CCGC 1 cut(s) 700
BspANI GGCC 1 cut(s) 29
BspCNI CTCAG 2 cut(s) 480, 633
BspEI TCCGGA 1 cut(s) 537
BspLI GGNNCC 2 cut(s) 536, 605
BspMI ACCTGC 1 cut(s) 830
BspPI GGATC 2 cut(s) 529, 542
BspTI CTTAAG 1 cut(s) 652
BsrDI GCAATG 1 cut(s) 181
BssECI CCNNGG 2 cut(s) 71, 736
BssMI GATC 1 cut(s) 534
BssT1I CCWWGG 1 cut(s) 736
Bst2UI CCWGG 2 cut(s) 73, 243
Bst4CI ACNGT 4 cut(s) 22, 40, 66, 305
Bst6I CTCTTC 2 cut(s) 38, 132
BstAFI CTTAAG 1 cut(s) 652
BstC8I GCNNGC 1 cut(s) 423
BstDEI CTNAG 3 cut(s) 369, 488, 620
BstF5I GGATG 1 cut(s) 517
BstKTI GATC 1 cut(s) 537
BstMAI GTCTC 1 cut(s) 806
BstMBI GATC 1 cut(s) 534
BstMWI GCNNNNNNNGC 1 cut(s) 427
BstNI CCWGG 2 cut(s) 73, 243
BstNSI RCATGY 1 cut(s) 403
BstSCI CCNGG 2 cut(s) 71, 241
BstV2I GAAGAC 1 cut(s) 160
BstX2I RGATCY 1 cut(s) 534
BstYI RGATCY 1 cut(s) 534
BsuRI GGCC 1 cut(s) 29
BtsCI GGATG 1 cut(s) 517
BtsIMutI CAGTG 1 cut(s) 45
BveI ACCTGC 1 cut(s) 830
Cac8I GCNNGC 1 cut(s) 423
Csp6I GTAC 3 cut(s) 131, 584, 775
CspCI CAANNNNNGTGG 2 cut(s) 97, 132
CviAII CATG 3 cut(s) 104, 400, 626
CviJI RGCY 9 cut(s) 29, 114, 266, 329, 425, 575, 624, 657, 745
CviKI_1 RGCY 9 cut(s) 29, 114, 266, 329, 425, 575, 624, 657, 745
CviQI GTAC 3 cut(s) 131, 584, 775
DdeI CTNAG 3 cut(s) 369, 488, 620
DpnI GATC 1 cut(s) 536
DpnII GATC 1 cut(s) 534
Eam1104I CTCTTC 2 cut(s) 38, 132
EarI CTCTTC 2 cut(s) 38, 132
Eco130I CCWWGG 1 cut(s) 736
Eco57I CTGAAG 2 cut(s) 154, 591
EcoRII CCWGG 2 cut(s) 71, 241
EcoT14I CCWWGG 1 cut(s) 736
ErhI CCWWGG 1 cut(s) 736
FaeI CATG 3 cut(s) 107, 403, 629
FalI AAGNNNNNCTT 2 cut(s) 410, 442
FatI CATG 3 cut(s) 103, 399, 625
Fnu4HI GCNGC 1 cut(s) 700
FokI GGATG 1 cut(s) 524
Fsp4HI GCNGC 1 cut(s) 700
FspBI CTAG 4 cut(s) 299, 737, 746, 869
GluI GCNGC 1 cut(s) 700
HaeIII GGCC 1 cut(s) 29
HapII CCGG 1 cut(s) 538
Hin1II CATG 3 cut(s) 107, 403, 629
HindIII AAGCTT 2 cut(s) 112, 573
HinfI GANTC 4 cut(s) 120, 233, 764, 889
HpaII CCGG 1 cut(s) 538
HphI GGTGA 1 cut(s) 290
Hpy166II GTNNAC 2 cut(s) 18, 62
Hpy188I TCNGA 3 cut(s) 293, 497, 571
Hpy188III TCNNGA 5 cut(s) 257, 344, 487, 538, 847
Hpy8I GTNNAC 2 cut(s) 18, 62
Hpy99I CGWCG 1 cut(s) 798
HpyAV CCTTC 3 cut(s) 467, 594, 844
HpyCH4III ACNGT 4 cut(s) 22, 40, 66, 305
HpyCH4IV ACGT 1 cut(s) 322
HpyCH4V TGCA 9 cut(s) 10, 86, 97, 430, 479, 527, 752, 833, 839
HpyF10VI GCNNNNNNNGC 1 cut(s) 427
HpyF3I CTNAG 3 cut(s) 369, 488, 620
HpySE526I ACGT 1 cut(s) 322
Hsp92II CATG 3 cut(s) 107, 403, 629
Kpn2I TCCGGA 1 cut(s) 537
Kzo9I GATC 1 cut(s) 534
LweI GCATC 4 cut(s) 95, 324, 466, 826
MaeI CTAG 4 cut(s) 299, 737, 746, 869
MaeII ACGT 1 cut(s) 322
MaeIII GTNAC 4 cut(s) 66, 383, 515, 544
MalI GATC 1 cut(s) 536
MboI GATC 1 cut(s) 534
MboII GAAGA 7 cut(s) 55, 61, 149, 160, 359, 374, 458
MfeI CAATTG 1 cut(s) 522
MflI RGATCY 1 cut(s) 534
MluCI AATT 3 cut(s) 522, 631, 906
MmeI TCCRAC 1 cut(s) 853
MnlI CCTC 6 cut(s) 39, 133, 428, 463, 590, 869
MroI TCCGGA 1 cut(s) 537
MroXI GAANNNNTTC 1 cut(s) 237
MseI TTAA 1 cut(s) 653
MslI CAYNNNNRTG 2 cut(s) 102, 258
MspCI CTTAAG 1 cut(s) 652
MspI CCGG 1 cut(s) 538
MspR9I CCNGG 2 cut(s) 73, 243
MunI CAATTG 1 cut(s) 522
Mva1269I GAATGC 1 cut(s) 97
MvaI CCWGG 2 cut(s) 73, 243
MwoI GCNNNNNNNGC 1 cut(s) 427
NdeII GATC 1 cut(s) 534
NlaIII CATG 3 cut(s) 107, 403, 629
NlaIV GGNNCC 2 cut(s) 536, 605
NmuCI GTSAC 2 cut(s) 383, 544
NspI RCATGY 1 cut(s) 403
PciI ACATGT 1 cut(s) 399
PctI GAATGC 1 cut(s) 97
PdmI GAANNNNTTC 1 cut(s) 237
PfeI GAWTC 4 cut(s) 120, 233, 764, 889
PfoI TCCNGGA 1 cut(s) 241
PkrI GCNGC 1 cut(s) 701
PscI ACATGT 1 cut(s) 399
Psp6I CCWGG 2 cut(s) 71, 241
PspGI CCWGG 2 cut(s) 71, 241
PspN4I GGNNCC 2 cut(s) 536, 605
PsuI RGATCY 1 cut(s) 534
RsaI GTAC 3 cut(s) 132, 585, 776
RsaNI GTAC 3 cut(s) 131, 584, 775
RseI CAYNNNNRTG 2 cut(s) 102, 258
SaqAI TTAA 1 cut(s) 653
SatI GCNGC 1 cut(s) 700
Sau3AI GATC 1 cut(s) 534
ScaI AGTACT 1 cut(s) 776
ScrFI CCNGG 2 cut(s) 73, 243
SfaNI GCATC 4 cut(s) 95, 324, 466, 826
SmiMI CAYNNNNRTG 2 cut(s) 102, 258
SmlI CTYRAG 2 cut(s) 31, 652
SmoI CTYRAG 2 cut(s) 31, 652
Sse9I AATT 3 cut(s) 522, 631, 906
SsiI CCGC 1 cut(s) 700
SspMI CTAG 4 cut(s) 299, 737, 746, 869
StyD4I CCNGG 2 cut(s) 71, 241
StyI CCWWGG 1 cut(s) 736
TaaI ACNGT 4 cut(s) 22, 40, 66, 305
TaiI ACGT 1 cut(s) 325
TasI AATT 3 cut(s) 522, 631, 906
TatI WGTACW 1 cut(s) 774
TauI GCSGC 1 cut(s) 702
TfiI GAWTC 4 cut(s) 120, 233, 764, 889
Tru1I TTAA 1 cut(s) 653
Tru9I TTAA 1 cut(s) 653
TscAI CASTG 1 cut(s) 45
TseFI GTSAC 2 cut(s) 383, 544
Tsp45I GTSAC 2 cut(s) 383, 544
TspDTI ATGAA 5 cut(s) 215, 263, 705, 876, 881
TspRI CASTG 1 cut(s) 45
Vha464I CTTAAG 1 cut(s) 652
XapI RAATTY 2 cut(s) 631, 906
XceI RCATGY 1 cut(s) 403
XmaJI CCTAGG 1 cut(s) 736
XmnI GAANNNNTTC 1 cut(s) 237
XspI CTAG 4 cut(s) 299, 737, 746, 869
ZrmI AGTACT 1 cut(s) 776
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.