MD14G1018900.v1.1

UPF0481 protein At3g47200-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr14
OG0002694
Physical Location & Seq
Forward (+)
1771964 .. 1773403
1440 bp
Loading structure...
UTR
Exon/CDS
Intron
MD14G1018900.v1.1.491

Sequence Viewer

Length: 1440 bp
ATGGGGGATCATGCTATCACAGTAGTAGATTCAAGTAGTAGTAGTAGTAGCGAGGAAGAAGATGAAGTGATGAATTACGAAGAAACTGTGGAAGCTATGCGAAGTGCTCTAGACAAGCACAGCAACGAAATTCAAACGAGTAGTAGAAGCTCATCATCAGTGTGCATATATAAAGTTCCTGCCAGCCTCGCTGGAATCCGTCCACAAGCCATTGAACCTGAAATGATCTCCATTGGACCTTACCACCGCAGCAAGGACGGGTTAATGGAACTTGAATCCTACAAATGGCGGTTCCTTGCTTCAATCCTTTCCCGAGAAAGGTATAAAAGAGGGCGTGATCTGCTCGTAGAGTACTACAAAGCCATGAGAAAGTTGGAGGAGCGTACGAGAAGCTGCTACTCCGAGTTGATGCCTGTGTCAAGCCCTGACTTTGTTCAAATGATGGTGCTTGATGGTTGCTTTATCATCGAGCTTTTTCGACAGAGGAATACAGATGAAGAGGACACCATTTTAAAAAGGCCGTTTTTTATTCAAATTTTAACTAGGGACCTTCTGAAGCTTCAAAACCAACTCCCTTTCTTCGTCCTTGAAAAACTATTTGAGATTTCCGACTTTGGAACTCAACATTCTCTATCCTTGCTTGCGCTTGAGTTCTTCAATCATTCGTTGCCTAGGCCTAGCGAAGTCCTCAAGAGGACGAGTGAGCTTACTGGGGGGGCCAACCACTTGCTCGACTTGTTTCGCTTAAGCTTCCTTCCTCCCGCACCGCACGATCCTCCAAGGAACAATCCTCCTCCCTCGAAGTCAATATTCCAACGAAAGGCAAATGCTCTACTGCAGATATTGATTTGCCTGTGTATGCTAGCAACTTCGATTGTAGTCGATGATGATCGTCACTCTAACCTAAAGTACCGCCCAACTGAGAAATCAATCCAATGTACCACACAACTAAGACTCTCTTGGGTAAAATTTAGGCCGCGCAAAAATGCGGAGAGCTTCTTAGACGTTGGTTACCAGAAAAGGGTACTTCGCATCCCACCTATAACCATCAATGAGCTCACCATTGCTATCTTTATCAATTGTATGGCCTTTGAACTCTGTCACCCACACACAGCTCCGCTCTTCACCGCTTATATTGCCTTCATGCGCTGTCTCATCAATTCGAATAGGGACGTCCAAGTTCTTTGTGCTGAAGGGATTATCGCCAGCTTCTCACAGAACGATCACAACGTAACTGAGCTGTTCACAAACTTGGGGGAAAAAGTTGTGCTCGACATAGGAGATTGCTACCTCTCCGAGCAATTCAGAGATGTGGAGGCCTATTACAGCAGCTGTAGGGGTACCTTCCTGCGAACTTGTTTTGGCGAGCCATGGTCGATCGCCTCATTTGTCTATGCCTTAATATTGTTTGCGTTCACTATGGTCACAGCTTCCAGATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF247

Protein Analysis

480

Amino Acids

54.88

Weight (kDa)

6.17

Isoelectric Point (pI)

50.12

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF247 PF03140 56 - 471 3.2e-102 Plant protein of unknown function
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 1176
Acc65I GGTACC 1 cut(s) 1340
AccB1I GGYRCC 1 cut(s) 1340
AccBSI CCGCTC 1 cut(s) 1120
AccII CGCG 1 cut(s) 979
AciI CCGC 9 cut(s) 247, 289, 762, 767, 913, 977, 989, 1118, 1128
AclWI GGATC 2 cut(s) 15, 767
AcsI RAATTY 3 cut(s) 129, 534, 968
AcuI CTGAAG 2 cut(s) 575, 1212
AcyI GRCGYC 1 cut(s) 1173
AfaI GTAC 6 cut(s) 353, 385, 911, 940, 1026, 1342
AfiI CCNNNNNNNGG 5 cut(s) 253, 285, 318, 820, 1021
AflII CTTAAG 1 cut(s) 745
Alw21I GWGCWC 3 cut(s) 109, 1059, 1272
Alw26I GTCTC 1 cut(s) 1157
AlwI GGATC 2 cut(s) 15, 767
AlwNI CAGNNNCTG 1 cut(s) 1332
Ama87I CYCGRG 1 cut(s) 312
AoxI GGCC 6 cut(s) 518, 674, 717, 974, 1086, 1317
ApeKI GCWGC 3 cut(s) 249, 393, 1329
ApoI RAATTY 3 cut(s) 129, 534, 968
Asp718I GGTACC 1 cut(s) 1340
AspA2I CCTAGG 1 cut(s) 671
AspLEI GCGC 3 cut(s) 646, 981, 1149
AspS9I GGNCC 3 cut(s) 236, 547, 717
AsuHPI GGTGA 3 cut(s) 1051, 1094, 1117
AsuII TTCGAA 1 cut(s) 1163
AsuNHI GCTAGC 1 cut(s) 862
AvaI CYCGRG 1 cut(s) 312
AvaII GGWCC 2 cut(s) 236, 547
AvrII CCTAGG 1 cut(s) 671
BanI GGYRCC 1 cut(s) 1340
BanII GRGCYC 1 cut(s) 1059
Bbv12I GWGCWC 3 cut(s) 109, 1059, 1272
BbvI GCAGC 3 cut(s) 261, 380, 1341
BccI CCATC 3 cut(s) 436, 446, 1055
BceAI ACGGC 1 cut(s) 505
BcoDI GTCTC 1 cut(s) 1157
BfaI CTAG 5 cut(s) 110, 543, 672, 678, 863
BfmI CTRYAG 2 cut(s) 836, 1333
BfrI CTTAAG 1 cut(s) 745
BisI GCNGC 4 cut(s) 250, 394, 977, 1330
BlnI CCTAGG 1 cut(s) 671
BlsI GCNGC 4 cut(s) 251, 395, 978, 1331
BmcAI AGTACT 1 cut(s) 353
Bme18I GGWCC 2 cut(s) 236, 547
BmeT110I CYCGRG 1 cut(s) 312
BmgT120I GGNCC 3 cut(s) 236, 547, 717
BmiI GGNNCC 4 cut(s) 293, 548, 718, 1342
BmrI ACTGGG 1 cut(s) 720
BmsI GCATC 2 cut(s) 399, 1041
BmtI GCTAGC 1 cut(s) 866
BmuI ACTGGG 1 cut(s) 720
Bpu14I TTCGAA 1 cut(s) 1163
BpuEI CTTGAG 2 cut(s) 668, 674
BsaBI GATNNNNATC 1 cut(s) 888
BsaHI GRCGYC 1 cut(s) 1173
BsaJI CCNNGG 3 cut(s) 671, 779, 1370
Bsc4I CCNNNNNNNGG 5 cut(s) 253, 285, 318, 820, 1021
Bse1I ACTGG 1 cut(s) 715
Bse3DI GCAATG 1 cut(s) 1062
Bse8I GATNNNNATC 1 cut(s) 888
BseDI CCNNGG 3 cut(s) 671, 779, 1370
BseGI GGATG 1 cut(s) 1032
BseJI GATNNNNATC 1 cut(s) 888
BseLI CCNNNNNNNGG 5 cut(s) 253, 285, 318, 820, 1021
BseMI GCAATG 1 cut(s) 1062
BseMII CTCAG 2 cut(s) 912, 1227
BseNI ACTGG 1 cut(s) 715
BseRI GAGGAG 2 cut(s) 392, 783
BseXI GCAGC 3 cut(s) 261, 380, 1341
Bsh1236I CGCG 1 cut(s) 979
Bsh1285I CGRYCG 1 cut(s) 1380
BshFI GGCC 6 cut(s) 520, 676, 719, 976, 1088, 1319
BshNI GGYRCC 1 cut(s) 1340
BsiEI CGRYCG 1 cut(s) 1380
BsiHKAI GWGCWC 3 cut(s) 109, 1059, 1272
BsiHKCI CYCGRG 1 cut(s) 312
BsiWI CGTACG 1 cut(s) 383
BslFI GGGAC 2 cut(s) 560, 1184
BslI CCNNNNNNNGG 5 cut(s) 253, 285, 318, 820, 1021
BsmAI GTCTC 1 cut(s) 1157
BsmFI GGGAC 2 cut(s) 560, 1184
BsnI GGCC 6 cut(s) 520, 676, 719, 976, 1088, 1319
BsoBI CYCGRG 1 cut(s) 312
Bsp119I TTCGAA 1 cut(s) 1163
Bsp1286I GDGCHC 3 cut(s) 109, 1059, 1272
Bsp143I GATC 7 cut(s) 7, 225, 337, 772, 889, 1222, 1377
Bsp19I CCATGG 1 cut(s) 1370
BspACI CCGC 9 cut(s) 247, 289, 762, 767, 913, 977, 989, 1118, 1128
BspANI GGCC 6 cut(s) 520, 676, 719, 976, 1088, 1319
BspCNI CTCAG 2 cut(s) 913, 1228
BspFNI CGCG 1 cut(s) 979
BspLI GGNNCC 4 cut(s) 293, 548, 718, 1342
BspMAI CTGCAG 1 cut(s) 840
BspOI GCTAGC 1 cut(s) 866
BspPI GGATC 2 cut(s) 15, 767
BspQI GCTCTTC 1 cut(s) 1127
BspT104I TTCGAA 1 cut(s) 1163
BspT107I GGYRCC 1 cut(s) 1340
BspTI CTTAAG 1 cut(s) 745
BsrBI CCGCTC 1 cut(s) 1120
BsrDI GCAATG 1 cut(s) 1062
BsrI ACTGG 1 cut(s) 715
BssECI CCNNGG 3 cut(s) 671, 779, 1370
BssMI GATC 7 cut(s) 7, 225, 337, 772, 889, 1222, 1377
BssNI GRCGYC 1 cut(s) 1173
BssT1I CCWWGG 3 cut(s) 671, 779, 1370
Bst4CI ACNGT 2 cut(s) 22, 88
Bst6I CTCTTC 2 cut(s) 492, 1127
BstACI GRCGYC 1 cut(s) 1173
BstAFI CTTAAG 1 cut(s) 745
BstBI TTCGAA 1 cut(s) 1163
BstC8I GCNNGC 5 cut(s) 184, 642, 864, 1207, 1367
BstDEI CTNAG 4 cut(s) 921, 950, 1000, 1236
BstDSI CCRYGG 1 cut(s) 1370
BstEII GGTNACC 1 cut(s) 1010
BstF5I GGATG 1 cut(s) 1032
BstFNI CGCG 1 cut(s) 979
BstHHI GCGC 3 cut(s) 646, 981, 1149
BstKTI GATC 7 cut(s) 10, 228, 340, 775, 892, 1225, 1380
BstMAI GTCTC 1 cut(s) 1157
BstMBI GATC 7 cut(s) 7, 225, 337, 772, 889, 1222, 1377
BstMCI CGRYCG 1 cut(s) 1380
BstMWI GCNNNNNNNGC 3 cut(s) 188, 340, 1136
BstPI GGTNACC 1 cut(s) 1010
BstSFI CTRYAG 2 cut(s) 836, 1333
BstUI CGCG 1 cut(s) 979
BstV1I GCAGC 3 cut(s) 261, 380, 1341
BsuRI GGCC 6 cut(s) 520, 676, 719, 976, 1088, 1319
BtgI CCRYGG 1 cut(s) 1370
BtsCI GGATG 1 cut(s) 1032
BtsIMutI CAGTG 1 cut(s) 165
Cac8I GCNNGC 5 cut(s) 184, 642, 864, 1207, 1367
CaiI CAGNNNCTG 1 cut(s) 1332
CfoI GCGC 3 cut(s) 646, 981, 1149
Cfr13I GGNCC 3 cut(s) 236, 547, 717
Csp6I GTAC 6 cut(s) 352, 384, 910, 939, 1025, 1341
CviAII CATG 4 cut(s) 11, 364, 1144, 1371
CviQI GTAC 6 cut(s) 352, 384, 910, 939, 1025, 1341
DdeI CTNAG 4 cut(s) 921, 950, 1000, 1236
DpnI GATC 7 cut(s) 9, 227, 339, 774, 891, 1224, 1379
DpnII GATC 7 cut(s) 7, 225, 337, 772, 889, 1222, 1377
DraI TTTAAA 1 cut(s) 513
Eam1104I CTCTTC 2 cut(s) 492, 1127
EarI CTCTTC 2 cut(s) 492, 1127
Ecl136II GAGCTC 1 cut(s) 1057
Eco130I CCWWGG 3 cut(s) 671, 779, 1370
Eco147I AGGCCT 2 cut(s) 676, 1319
Eco24I GRGCYC 1 cut(s) 1059
Eco47I GGWCC 2 cut(s) 236, 547
Eco53kI GAGCTC 1 cut(s) 1057
Eco57I CTGAAG 2 cut(s) 575, 1212
Eco88I CYCGRG 1 cut(s) 312
Eco91I GGTNACC 1 cut(s) 1010
EcoICRI GAGCTC 1 cut(s) 1057
EcoO109I RGGNCCY 1 cut(s) 547
EcoO65I GGTNACC 1 cut(s) 1010
EcoT14I CCWWGG 3 cut(s) 671, 779, 1370
EcoT38I GRGCYC 1 cut(s) 1059
ErhI CCWWGG 3 cut(s) 671, 779, 1370
FaeI CATG 4 cut(s) 14, 367, 1147, 1374
FalI AAGNNNNNCTT 2 cut(s) 943, 975
FaqI GGGAC 2 cut(s) 560, 1184
FatI CATG 4 cut(s) 10, 363, 1143, 1370
FauI CCCGC 1 cut(s) 769
Fnu4HI GCNGC 4 cut(s) 250, 394, 977, 1330
FokI GGATG 1 cut(s) 1019
FriOI GRGCYC 1 cut(s) 1059
Fsp4HI GCNGC 4 cut(s) 250, 394, 977, 1330
FspBI CTAG 5 cut(s) 110, 543, 672, 678, 863
GlaI GCGC 3 cut(s) 645, 980, 1148
GluI GCNGC 4 cut(s) 250, 394, 977, 1330
HaeIII GGCC 6 cut(s) 520, 676, 719, 976, 1088, 1319
HhaI GCGC 3 cut(s) 646, 981, 1149
Hin1I GRCGYC 1 cut(s) 1173
Hin1II CATG 4 cut(s) 14, 367, 1147, 1374
Hin6I GCGC 3 cut(s) 644, 979, 1147
HinP1I GCGC 3 cut(s) 644, 979, 1147
HindIII AAGCTT 2 cut(s) 557, 748
HinfI GANTC 4 cut(s) 29, 195, 275, 954
HphI GGTGA 3 cut(s) 1051, 1094, 1117
Hpy166II GTNNAC 3 cut(s) 203, 1245, 1416
Hpy188I TCNGA 5 cut(s) 403, 555, 610, 1297, 1307
Hpy188III TCNNGA 4 cut(s) 110, 312, 691, 1434
Hpy8I GTNNAC 3 cut(s) 203, 1245, 1416
HpyAV CCTTC 5 cut(s) 560, 764, 1150, 1187, 1354
HpyCH4III ACNGT 2 cut(s) 22, 88
HpyCH4IV ACGT 3 cut(s) 1005, 1173, 1230
HpyCH4V TGCA 2 cut(s) 165, 838
HpyF10VI GCNNNNNNNGC 3 cut(s) 188, 340, 1136
HpyF3I CTNAG 4 cut(s) 921, 950, 1000, 1236
HpySE526I ACGT 3 cut(s) 1005, 1173, 1230
Hsp92I GRCGYC 1 cut(s) 1173
Hsp92II CATG 4 cut(s) 14, 367, 1147, 1374
HspAI GCGC 3 cut(s) 644, 979, 1147
KpnI GGTACC 1 cut(s) 1344
Kzo9I GATC 7 cut(s) 7, 225, 337, 772, 889, 1222, 1377
LguI GCTCTTC 1 cut(s) 1127
LmnI GCTCC 2 cut(s) 379, 1120
Lsp1109I GCAGC 3 cut(s) 261, 380, 1341
LweI GCATC 2 cut(s) 399, 1041
MaeI CTAG 5 cut(s) 110, 543, 672, 678, 863
MaeII ACGT 3 cut(s) 1005, 1173, 1230
MaeIII GTNAC 5 cut(s) 893, 1010, 1100, 1231, 1423
MalI GATC 7 cut(s) 9, 227, 339, 774, 891, 1224, 1379
MbiI CCGCTC 1 cut(s) 1120
MboI GATC 7 cut(s) 7, 225, 337, 772, 889, 1222, 1377
MboII GAAGA 7 cut(s) 68, 71, 92, 509, 571, 646, 1114
MfeI CAATTG 1 cut(s) 1078
MhlI GDGCHC 3 cut(s) 109, 1059, 1272
MluCI AATT 7 cut(s) 73, 129, 534, 968, 1078, 1159, 1301
MlyI GAGTC 1 cut(s) 948
MmeI TCCRAC 3 cut(s) 354, 633, 838
MseI TTAA 5 cut(s) 263, 512, 539, 746, 1400
MslI CAYNNNNRTG 1 cut(s) 160
MspA1I CMGCKG 1 cut(s) 1332
MspCI CTTAAG 1 cut(s) 745
MunI CAATTG 1 cut(s) 1078
MvnI CGCG 1 cut(s) 979
MwoI GCNNNNNNNGC 3 cut(s) 188, 340, 1136
NcoI CCATGG 1 cut(s) 1370
NdeII GATC 7 cut(s) 7, 225, 337, 772, 889, 1222, 1377
NheI GCTAGC 1 cut(s) 862
NlaIII CATG 4 cut(s) 14, 367, 1147, 1374
NlaIV GGNNCC 4 cut(s) 293, 548, 718, 1342
NmuCI GTSAC 3 cut(s) 893, 1100, 1423
NspV TTCGAA 1 cut(s) 1163
PceI AGGCCT 2 cut(s) 676, 1319
PciSI GCTCTTC 1 cut(s) 1127
PcsI WCGNNNNNNNCGW 1 cut(s) 1227
PfeI GAWTC 3 cut(s) 29, 195, 275
Pfl23II CGTACG 1 cut(s) 383
PkrI GCNGC 4 cut(s) 251, 395, 978, 1331
Ple19I CGATCG 1 cut(s) 1380
PleI GAGTC 1 cut(s) 948
PpsI GAGTC 1 cut(s) 948
PpuMI RGGWCCY 1 cut(s) 547
Psp124BI GAGCTC 1 cut(s) 1059
Psp5II RGGWCCY 1 cut(s) 547
PspEI GGTNACC 1 cut(s) 1010
PspLI CGTACG 1 cut(s) 383
PspN4I GGNNCC 4 cut(s) 293, 548, 718, 1342
PspPI GGNCC 3 cut(s) 236, 547, 717
PspPPI RGGWCCY 1 cut(s) 547
PstI CTGCAG 1 cut(s) 840
PstNI CAGNNNCTG 1 cut(s) 1332
PvuI CGATCG 1 cut(s) 1380
PvuII CAGCTG 1 cut(s) 1332
RsaI GTAC 6 cut(s) 353, 385, 911, 940, 1026, 1342
RsaNI GTAC 6 cut(s) 352, 384, 910, 939, 1025, 1341
RseI CAYNNNNRTG 1 cut(s) 160
SacI GAGCTC 1 cut(s) 1059
SapI GCTCTTC 1 cut(s) 1127
SaqAI TTAA 5 cut(s) 263, 512, 539, 746, 1400
SatI GCNGC 4 cut(s) 250, 394, 977, 1330
Sau3AI GATC 7 cut(s) 7, 225, 337, 772, 889, 1222, 1377
Sau96I GGNCC 3 cut(s) 236, 547, 717
ScaI AGTACT 1 cut(s) 353
SchI GAGTC 1 cut(s) 948
SduI GDGCHC 3 cut(s) 109, 1059, 1272
SfaNI GCATC 2 cut(s) 399, 1041
SfcI CTRYAG 2 cut(s) 836, 1333
SfuI TTCGAA 1 cut(s) 1163
SinI GGWCC 2 cut(s) 236, 547
SmiMI CAYNNNNRTG 1 cut(s) 160
SmlI CTYRAG 3 cut(s) 647, 689, 745
SmoI CTYRAG 3 cut(s) 647, 689, 745
Sse9I AATT 7 cut(s) 73, 129, 534, 968, 1078, 1159, 1301
SseBI AGGCCT 2 cut(s) 676, 1319
SsiI CCGC 9 cut(s) 247, 289, 762, 767, 913, 977, 989, 1118, 1128
SspI AATATT 2 cut(s) 810, 1404
SspMI CTAG 5 cut(s) 110, 543, 672, 678, 863
SstI GAGCTC 1 cut(s) 1059
StuI AGGCCT 2 cut(s) 676, 1319
StyI CCWWGG 3 cut(s) 671, 779, 1370
TaaI ACNGT 2 cut(s) 22, 88
TaiI ACGT 3 cut(s) 1008, 1176, 1233
TaqI TCGA 9 cut(s) 468, 478, 732, 800, 872, 882, 1163, 1272, 1376
TasI AATT 7 cut(s) 73, 129, 534, 968, 1078, 1159, 1301
TatI WGTACW 1 cut(s) 351
TauI GCSGC 1 cut(s) 979
TfiI GAWTC 3 cut(s) 29, 195, 275
Tru1I TTAA 5 cut(s) 263, 512, 539, 746, 1400
Tru9I TTAA 5 cut(s) 263, 512, 539, 746, 1400
TscAI CASTG 1 cut(s) 165
TseFI GTSAC 3 cut(s) 893, 1100, 1423
TseI GCWGC 3 cut(s) 249, 393, 1329
Tsp45I GTSAC 3 cut(s) 893, 1100, 1423
TspDTI ATGAA 4 cut(s) 78, 86, 510, 1132
TspGWI ACGGA 1 cut(s) 188
TspRI CASTG 1 cut(s) 165
Vha464I CTTAAG 1 cut(s) 745
VpaK11BI GGWCC 2 cut(s) 236, 547
XapI RAATTY 3 cut(s) 129, 534, 968
XbaI TCTAGA 1 cut(s) 109
XcmI CCANNNNNNNNNTGG 1 cut(s) 370
XmaJI CCTAGG 1 cut(s) 671
XspI CTAG 5 cut(s) 110, 543, 672, 678, 863
ZraI GACGTC 1 cut(s) 1174
ZrmI AGTACT 1 cut(s) 353
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.