pycom12g02520

UPF0481 protein At3g47200-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr12
OG0002694
Physical Location & Seq
Forward (+)
2197411 .. 2198712
1302 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom12g02520.1

Sequence Viewer

Length: 1302 bp
ATGGATGCTGATCTTGAAAAAGGGCATGGATTAAGCCCTCCAGCTTCTGCGGGAAAGCACAAAGAAATAGAAGCAGCTCCCGATGATAGTAATATAATAGATCACCCTGGCAATGAAAAAGAAGAAGATGGGACCTTGGTGTCCATATACAAAGTTCCTGCAAGCCTTGCCGGACTCAATTCCAAAGCCGTTGAGCCAGAAAAGGTCTTCATGGGGCCATGCCACGGCGACAAGAGAACGTCGTCGAAATTTGAGAGCTGCAAGCGTTGGTATCTTCGTGCACTCCTTACTCGATCAAACCGTCCTGAAGCAGATTACTCCGAAGCCATGAGAAGGTTGGAGGGAAATACAAGAAGTTGCTACTCTGAGGATCATCGTGAGGTCATCTCCAAGTTGAAGGCAGATGAATTTATAGATATGATGATGCTTGATGGGTGCTTTATCATTGAGCTTTTTCATCGTATAAATACGGAAGATCTCACGGACGAAAACGATCCCATCTTAAGCAAGCCATGGTTGATTCCAATTGTCACCGGGGACCTCCTCAAGCTTGAAAATCAGATCCCTTTCTCCGTCCTCCAAGAATTACACAACATCTCCACGAATCAACGTCAAGAAGCCTACTCTCTAGTCCTTCTTGCGCTGAATTTGTTCAATAATTCGTTGCATCGGCCTATTGAAGTCCTTAAAAAGTCACAACCGTCAGAGGACCCCAAGCACTTGCTTGACTTGTTTTACTCAAGCCTTATTCCGCAAAAACATACTGTCCCGAATGGTCCATCGACCCGAAAAAACAAAAAAAAAGAACCAAAGTACTGTCCATCCTCCGAGTCAATCCAGTGTACCGTACAACTAAGATCCTCCGGTATAAAGTTCAAGCCACAAAGAGCGGACAGCTTCTTGGAGATCAATTTCCAAGGTAGCGTTATTAGCATGTGTAAGGAACTACAAATCCCACCTATAACAATCAACGATTTCACCACTACCCTCTTCATCAACTGCATGGCCTTGGAACAATGCCGCCAAAACAGTCCTACGTGGTTCACCACCTATATTGCTTTCATGAGCAGTCTCATCACATCACCTAGAGACGTCACATTCCTTTGTTCTGATGGGATCGTCACTACTGGCTTCTCATACAACGACCAAAACGTGGCAGAGCTGTTCAAAAATTTGGGGGAAAAAGCTGGCTTCAACAGAAGAGAATGCTACCTCTCCAAGCAATTCACAGATGTGGAAGCATATTACAGCAGCCGTTGGGCTACTTTCATCCGAAATTATTTTAGCAGGGCATGGTCCTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF247

Protein Analysis

434

Amino Acids

49.29

Weight (kDa)

6.11

Isoelectric Point (pI)

52.5

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF247 PF03140 49 - 433 6.2e-96 Plant protein of unknown function
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 139
AatII GACGTC 1 cut(s) 1095
AccB7I CCANNNNNTGG 1 cut(s) 1153
AccBSI CCGCTC 1 cut(s) 890
AciI CCGC 4 cut(s) 50, 752, 890, 1021
AclWI GGATC 5 cut(s) 378, 488, 556, 852, 1124
AcsI RAATTY 4 cut(s) 248, 407, 646, 1171
AcuI CTGAAG 1 cut(s) 327
AcyI GRCGYC 1 cut(s) 1092
AfaI GTAC 3 cut(s) 815, 844, 849
AfiI CCNNNNNNNGG 3 cut(s) 224, 333, 1153
AflII CTTAAG 1 cut(s) 502
AgsI TTSAA 8 cut(s) 17, 397, 554, 655, 680, 877, 1168, 1195
AjnI CCWGG 1 cut(s) 106
AleI CACNNNNGTG 1 cut(s) 1232
AloI GAACNNNNNNTCC 2 cut(s) 936, 968
AluBI AGCT 8 cut(s) 44, 77, 258, 451, 550, 897, 1162, 1187
AluI AGCT 8 cut(s) 44, 77, 258, 451, 550, 897, 1162, 1187
Alw21I GWGCWC 1 cut(s) 283
Alw26I GTCTC 2 cut(s) 1076, 1083
Alw44I GTGCAC 1 cut(s) 279
AlwI GGATC 5 cut(s) 378, 488, 556, 852, 1124
AlwNI CAGNNNCTG 1 cut(s) 47
AoxI GGCC 3 cut(s) 215, 671, 1005
ApaLI GTGCAC 1 cut(s) 279
ApeKI GCWGC 3 cut(s) 74, 258, 1251
ApoI RAATTY 4 cut(s) 248, 407, 646, 1171
Asp700I GAANNNNTTC 1 cut(s) 650
AspLEI GCGC 1 cut(s) 643
AspS9I GGNCC 6 cut(s) 132, 215, 538, 709, 776, 1296
AsuC2I CCSGG 1 cut(s) 535
AsuHPI GGTGA 5 cut(s) 95, 523, 970, 1036, 1074
AvaII GGWCC 5 cut(s) 132, 538, 709, 776, 1296
BaeGI GKGCMC 1 cut(s) 283
BbsI GAAGAC 1 cut(s) 199
Bbv12I GWGCWC 1 cut(s) 283
BbvI GCAGC 3 cut(s) 86, 245, 1263
BccI CCATC 6 cut(s) 122, 425, 506, 787, 829, 1106
BceAI ACGGC 3 cut(s) 173, 241, 1239
BciT130I CCWGG 1 cut(s) 108
BcnI CCSGG 1 cut(s) 535
BcoDI GTCTC 2 cut(s) 1076, 1083
BfaI CTAG 2 cut(s) 629, 1086
BfrI CTTAAG 1 cut(s) 502
BglII AGATCT 1 cut(s) 475
BisI GCNGC 4 cut(s) 75, 259, 1021, 1252
BlsI GCNGC 4 cut(s) 76, 260, 1022, 1253
BmcAI AGTACT 1 cut(s) 815
Bme1390I CCNGG 2 cut(s) 108, 535
Bme18I GGWCC 5 cut(s) 132, 538, 709, 776, 1296
BmgT120I GGNCC 6 cut(s) 132, 215, 538, 709, 776, 1296
BmiI GGNNCC 4 cut(s) 133, 216, 539, 711
BmrFI CCNGG 2 cut(s) 108, 535
BmsI GCATC 2 cut(s) 414, 676
BpiI GAAGAC 1 cut(s) 199
BplI GAGNNNNNCTC 2 cut(s) 371, 403
BpmI CTGGAG 1 cut(s) 24
BpuEI CTTGAG 2 cut(s) 530, 724
BpuMI CCSGG 1 cut(s) 535
BsaAI YACGTR 1 cut(s) 1038
BsaBI GATNNNNATC 1 cut(s) 9
BsaHI GRCGYC 1 cut(s) 1092
BsaJI CCNNGG 7 cut(s) 106, 135, 223, 512, 534, 916, 1008
BsaWI WCCGGW 1 cut(s) 863
BsaXI ACNNNNNCTCC 2 cut(s) 581, 611
Bsc4I CCNNNNNNNGG 3 cut(s) 224, 333, 1153
Bse1I ACTGG 2 cut(s) 838, 1132
Bse3DI GCAATG 1 cut(s) 118
Bse8I GATNNNNATC 1 cut(s) 9
BseBI CCWGG 1 cut(s) 108
BseDI CCNNGG 7 cut(s) 106, 135, 223, 512, 534, 916, 1008
BseGI GGATG 3 cut(s) 10, 821, 1269
BseJI GATNNNNATC 1 cut(s) 9
BseLI CCNNNNNNNGG 3 cut(s) 224, 333, 1153
BseMI GCAATG 1 cut(s) 118
BseMII CTCAG 1 cut(s) 357
BseNI ACTGG 2 cut(s) 838, 1132
BseRI GAGGAG 1 cut(s) 533
BseSI GKGCMC 1 cut(s) 283
BseXI GCAGC 3 cut(s) 86, 245, 1263
BshFI GGCC 3 cut(s) 217, 673, 1007
BsiHKAI GWGCWC 1 cut(s) 283
BsiSI CCGG 3 cut(s) 171, 534, 864
BslFI GGGAC 3 cut(s) 145, 551, 752
BslI CCNNNNNNNGG 3 cut(s) 224, 333, 1153
BsmAI GTCTC 2 cut(s) 1076, 1083
BsmBI CGTCTC 1 cut(s) 1083
BsmFI GGGAC 3 cut(s) 145, 551, 752
BsmI GAATGC 1 cut(s) 1211
BsnI GGCC 3 cut(s) 217, 673, 1007
Bsp1286I GDGCHC 1 cut(s) 283
Bsp19I CCATGG 1 cut(s) 512
BspACI CCGC 4 cut(s) 50, 752, 890, 1021
BspANI GGCC 3 cut(s) 217, 673, 1007
BspCNI CTCAG 1 cut(s) 358
BspHI TCATGA 1 cut(s) 1062
BspLI GGNNCC 4 cut(s) 133, 216, 539, 711
BspPI GGATC 5 cut(s) 378, 488, 556, 852, 1124
BspTI CTTAAG 1 cut(s) 502
BsrBI CCGCTC 1 cut(s) 890
BsrDI GCAATG 1 cut(s) 118
BsrI ACTGG 2 cut(s) 838, 1132
BssECI CCNNGG 7 cut(s) 106, 135, 223, 512, 534, 916, 1008
BssNI GRCGYC 1 cut(s) 1092
BssT1I CCWWGG 4 cut(s) 135, 512, 916, 1008
Bst2UI CCWGG 1 cut(s) 108
Bst4CI ACNGT 6 cut(s) 302, 702, 766, 818, 847, 1031
Bst6I CTCTTC 2 cut(s) 995, 1195
BstACI GRCGYC 1 cut(s) 1092
BstAFI CTTAAG 1 cut(s) 502
BstAPI GCANNNNNTGC 1 cut(s) 167
BstBAI YACGTR 1 cut(s) 1038
BstC8I GCNNGC 4 cut(s) 163, 263, 509, 1189
BstDEI CTNAG 2 cut(s) 366, 854
BstDSI CCRYGG 2 cut(s) 223, 512
BstF5I GGATG 3 cut(s) 10, 821, 1269
BstHHI GCGC 1 cut(s) 643
BstMAI GTCTC 2 cut(s) 1076, 1083
BstMWI GCNNNNNNNGC 2 cut(s) 167, 930
BstNI CCWGG 1 cut(s) 108
BstNSI RCATGY 1 cut(s) 937
BstSCI CCNGG 2 cut(s) 106, 533
BstSLI GKGCMC 1 cut(s) 283
BstV1I GCAGC 3 cut(s) 86, 245, 1263
BstV2I GAAGAC 1 cut(s) 199
BstX2I RGATCY 3 cut(s) 475, 561, 857
BstYI RGATCY 3 cut(s) 475, 561, 857
BsuRI GGCC 3 cut(s) 217, 673, 1007
BtgI CCRYGG 2 cut(s) 223, 512
BtsCI GGATG 3 cut(s) 10, 821, 1269
BtsIMutI CAGTG 1 cut(s) 845
Cac8I GCNNGC 4 cut(s) 163, 263, 509, 1189
CaiI CAGNNNCTG 1 cut(s) 47
CciI TCATGA 1 cut(s) 1062
CfoI GCGC 1 cut(s) 643
Cfr13I GGNCC 6 cut(s) 132, 215, 538, 709, 776, 1296
Csp6I GTAC 3 cut(s) 814, 843, 848
CspCI CAANNNNNGTGG 2 cut(s) 1036, 1071
CviAII CATG 9 cut(s) 26, 211, 219, 328, 513, 934, 1003, 1063, 1293
CviQI GTAC 3 cut(s) 814, 843, 848
DdeI CTNAG 2 cut(s) 366, 854
DrdI GACNNNNNNGTC 1 cut(s) 139
DseDI GACNNNNNNGTC 1 cut(s) 139
Eam1104I CTCTTC 2 cut(s) 995, 1195
EarI CTCTTC 2 cut(s) 995, 1195
Eco130I CCWWGG 4 cut(s) 135, 512, 916, 1008
Eco47I GGWCC 5 cut(s) 132, 538, 709, 776, 1296
Eco57I CTGAAG 1 cut(s) 327
EcoO109I RGGNCCY 3 cut(s) 132, 538, 709
EcoRII CCWGG 1 cut(s) 106
EcoT14I CCWWGG 4 cut(s) 135, 512, 916, 1008
ErhI CCWWGG 4 cut(s) 135, 512, 916, 1008
Esp3I CGTCTC 1 cut(s) 1083
FaeI CATG 9 cut(s) 29, 214, 222, 331, 516, 937, 1006, 1066, 1296
FaqI GGGAC 3 cut(s) 145, 551, 752
FatI CATG 9 cut(s) 25, 210, 218, 327, 512, 933, 1002, 1062, 1292
FauI CCCGC 1 cut(s) 43
Fnu4HI GCNGC 4 cut(s) 75, 259, 1021, 1252
FokI GGATG 3 cut(s) 17, 808, 1256
Fsp4HI GCNGC 4 cut(s) 75, 259, 1021, 1252
FspBI CTAG 2 cut(s) 629, 1086
GlaI GCGC 1 cut(s) 642
GluI GCNGC 4 cut(s) 75, 259, 1021, 1252
GsuI CTGGAG 1 cut(s) 24
HaeIII GGCC 3 cut(s) 217, 673, 1007
HapII CCGG 3 cut(s) 171, 534, 864
HhaI GCGC 1 cut(s) 643
Hin1I GRCGYC 1 cut(s) 1092
Hin1II CATG 9 cut(s) 29, 214, 222, 331, 516, 937, 1006, 1066, 1296
Hin6I GCGC 1 cut(s) 641
HinP1I GCGC 1 cut(s) 641
HindIII AAGCTT 1 cut(s) 548
HinfI GANTC 4 cut(s) 174, 520, 604, 830
HpaII CCGG 3 cut(s) 171, 534, 864
HphI GGTGA 5 cut(s) 95, 523, 970, 1036, 1074
Hpy166II GTNNAC 3 cut(s) 281, 843, 1044
Hpy188I TCNGA 7 cut(s) 322, 367, 561, 706, 829, 1111, 1274
Hpy188III TCNNGA 7 cut(s) 14, 80, 305, 377, 614, 769, 1063
Hpy8I GTNNAC 3 cut(s) 281, 843, 1044
Hpy99I CGWCG 2 cut(s) 244, 247
HpyAV CCTTC 3 cut(s) 327, 391, 644
HpyCH4III ACNGT 6 cut(s) 302, 702, 766, 818, 847, 1031
HpyCH4IV ACGT 5 cut(s) 239, 610, 1037, 1092, 1152
HpyCH4V TGCA 5 cut(s) 161, 261, 281, 667, 1002
HpyF10VI GCNNNNNNNGC 2 cut(s) 167, 930
HpyF3I CTNAG 2 cut(s) 366, 854
HpySE526I ACGT 5 cut(s) 239, 610, 1037, 1092, 1152
Hsp92I GRCGYC 1 cut(s) 1092
Hsp92II CATG 9 cut(s) 29, 214, 222, 331, 516, 937, 1006, 1066, 1296
HspAI GCGC 1 cut(s) 641
LmnI GCTCC 1 cut(s) 82
Lsp1109I GCAGC 3 cut(s) 86, 245, 1263
LweI GCATC 2 cut(s) 414, 676
MaeI CTAG 2 cut(s) 629, 1086
MaeII ACGT 5 cut(s) 239, 610, 1037, 1092, 1152
MaeIII GTNAC 4 cut(s) 529, 693, 1093, 1120
MbiI CCGCTC 1 cut(s) 890
MboII GAAGA 7 cut(s) 134, 137, 199, 266, 485, 982, 1212
MfeI CAATTG 1 cut(s) 525
MflI RGATCY 3 cut(s) 475, 561, 857
MhlI GDGCHC 1 cut(s) 283
MlyI GAGTC 2 cut(s) 168, 839
MmeI TCCRAC 1 cut(s) 318
MroXI GAANNNNTTC 1 cut(s) 650
MseI TTAA 3 cut(s) 32, 503, 687
MslI CAYNNNNRTG 1 cut(s) 1232
MspCI CTTAAG 1 cut(s) 502
MspI CCGG 3 cut(s) 171, 534, 864
MspR9I CCNGG 2 cut(s) 108, 535
MunI CAATTG 1 cut(s) 525
Mva1269I GAATGC 1 cut(s) 1211
MvaI CCWGG 1 cut(s) 108
MwoI GCNNNNNNNGC 2 cut(s) 167, 930
NciI CCSGG 1 cut(s) 535
NcoI CCATGG 1 cut(s) 512
NlaIII CATG 9 cut(s) 29, 214, 222, 331, 516, 937, 1006, 1066, 1296
NlaIV GGNNCC 4 cut(s) 133, 216, 539, 711
NmuCI GTSAC 4 cut(s) 529, 693, 1093, 1120
NspI RCATGY 1 cut(s) 937
OliI CACNNNNGTG 1 cut(s) 1232
PagI TCATGA 1 cut(s) 1062
PcsI WCGNNNNNNNCGW 2 cut(s) 298, 1149
PctI GAATGC 1 cut(s) 1211
PdmI GAANNNNTTC 1 cut(s) 650
PfeI GAWTC 2 cut(s) 520, 604
PflMI CCANNNNNTGG 1 cut(s) 1153
PkrI GCNGC 4 cut(s) 76, 260, 1022, 1253
PleI GAGTC 2 cut(s) 168, 838
PpsI GAGTC 2 cut(s) 168, 838
Ppu21I YACGTR 1 cut(s) 1038
PpuMI RGGWCCY 3 cut(s) 132, 538, 709
Psp5II RGGWCCY 3 cut(s) 132, 538, 709
Psp6I CCWGG 1 cut(s) 106
PspGI CCWGG 1 cut(s) 106
PspN4I GGNNCC 4 cut(s) 133, 216, 539, 711
PspPI GGNCC 6 cut(s) 132, 215, 538, 709, 776, 1296
PspPPI RGGWCCY 3 cut(s) 132, 538, 709
PstNI CAGNNNCTG 1 cut(s) 47
PsuI RGATCY 3 cut(s) 475, 561, 857
RsaI GTAC 3 cut(s) 815, 844, 849
RsaNI GTAC 3 cut(s) 814, 843, 848
RseI CAYNNNNRTG 1 cut(s) 1232
SaqAI TTAA 3 cut(s) 32, 503, 687
SatI GCNGC 4 cut(s) 75, 259, 1021, 1252
Sau96I GGNCC 6 cut(s) 132, 215, 538, 709, 776, 1296
ScaI AGTACT 1 cut(s) 815
SchI GAGTC 2 cut(s) 168, 839
ScrFI CCNGG 2 cut(s) 108, 535
SduI GDGCHC 1 cut(s) 283
SfaNI GCATC 2 cut(s) 414, 676
SinI GGWCC 5 cut(s) 132, 538, 709, 776, 1296
SmiMI CAYNNNNRTG 1 cut(s) 1232
SmlI CTYRAG 3 cut(s) 502, 545, 739
SmoI CTYRAG 3 cut(s) 502, 545, 739
SsiI CCGC 4 cut(s) 50, 752, 890, 1021
SspMI CTAG 2 cut(s) 629, 1086
StyD4I CCNGG 2 cut(s) 106, 533
StyI CCWWGG 4 cut(s) 135, 512, 916, 1008
TaaI ACNGT 6 cut(s) 302, 702, 766, 818, 847, 1031
TaiI ACGT 5 cut(s) 242, 613, 1040, 1095, 1155
TaqI TCGA 3 cut(s) 245, 292, 782
TatI WGTACW 1 cut(s) 813
TauI GCSGC 1 cut(s) 1023
TfiI GAWTC 2 cut(s) 520, 604
Tru1I TTAA 3 cut(s) 32, 503, 687
Tru9I TTAA 3 cut(s) 32, 503, 687
TscAI CASTG 1 cut(s) 845
TseFI GTSAC 4 cut(s) 529, 693, 1093, 1120
TseI GCWGC 3 cut(s) 74, 258, 1251
Tsp45I GTSAC 4 cut(s) 529, 693, 1093, 1120
TspDTI ATGAA 7 cut(s) 129, 199, 420, 446, 982, 1051, 1258
TspGWI ACGGA 3 cut(s) 485, 497, 562
TspRI CASTG 1 cut(s) 845
Van91I CCANNNNNTGG 1 cut(s) 1153
Vha464I CTTAAG 1 cut(s) 502
VneI GTGCAC 1 cut(s) 279
VpaK11BI GGWCC 5 cut(s) 132, 538, 709, 776, 1296
XapI RAATTY 4 cut(s) 248, 407, 646, 1171
XceI RCATGY 1 cut(s) 937
XcmI CCANNNNNNNNNTGG 1 cut(s) 334
XmnI GAANNNNTTC 1 cut(s) 650
XspI CTAG 2 cut(s) 629, 1086
ZraI GACGTC 1 cut(s) 1093
ZrmI AGTACT 1 cut(s) 815
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.