RLG00000024068

UPF0481 protein At3g47200-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr5
OG0002694
Physical Location & Seq
Reverse (-)
31452266 .. 31454294
2029 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000024068

Sequence Viewer

Length: 1569 bp
ATGGTAGAGCTAACTGATGCCGATCAGGAACGAGTGGTGATCGATATTGACGAATCTGTAGCTGCCATGCAAAGGCGGCTACAGGAAAGCAGATGCGGAAGTCGGAGAGAATCAATATATATGGACAACTTTAGTTCAGTTGTCTGCATATACAGAGTTCCTGAAAGCCTCGTGGGAATCAGTCCAAAAGCTATAAGGCCTGAAAAGGTCTCCATCGGACCTTATCACCACAGCAAGAGCAAGGAATCTGAAGAGACCAAAATGAAGTATTTTGAAGCTTTTCTCTCTAGAAAAAAGGGAGATGGTAGTGCAGGACCTGATCTAAGTACCTTGTTTAAAGCCGCGAGAAATATGGAGGCAGAAGCAAGAAGATGCTATGATGATCAGGCCATCGCTATGTCAAGCGCGGAGTTTGTTGCAATGATGGTACTTGATGGGTGCTTTATCATTGAGCTTTTCCTACAAGACCAAGAGGACAAACTCAAAGATGTTGAAGAGAAGGACCGCCTGGTTATGGACCTCCTCAAGCTTGAAAACCAACTGCCTTTATTCGTCCTTGAAAGACTACTCAGCCTGTCCAGTGATTCATCTGTACCAGGTTCTCTAGCCTTGCTTGCCTTGAAGTTCTTCAATGTTTATGATCACAAAGTCCTGGATGATGTTAAACACTTGTTACACTTGTTTCACTCAAGCTTCCGTACCCCTTTATTATTAGAACGAGATAATACTCGATATCAGCTCTCTAATATTAGAATGCGCTGCGTAGACTTACTTGGGGAAGCTGATGATGTAATGATTGCATATGTAACGACATGCCTATCAATCGACAGCCCAGCGACCGCAATGAGGTGCTTCCTTGCAACCCCTTTTCTATTTATTTGGGGGATCCTTGCATTCCTTTATCGTCTTCTAAGGTTAGTCGGTTCAATCCTTTTCTTCTTTGTTTGGTGGAACGTGGATAACGTTCAAAGATTCCTTCACCCCACATGTCAGGAGCATCGTCCTTTCTCCGATTCAATCCAATGTGCCACACAACTAAGACCCTCCGGGATCATTTTCAAGCCACTCCTAGCAGAAAGCTTCTTAACTATCGATTACCACAAACGGGTACTGCAAATACCACCCCTAACCATCAATGATGTCACTATTACTATCTTCATCAACAGCATAGCCTTTGAACAATGTTGCCCACACAAGACTGGTTCTTTATTTACCACTTACATTGCCTTCATGAGCTCTCTCATCAATACGCCTAGAGACGTCGCATTGCTTTCTGGTAACGGGATTATCATTACCAGCTTCTCACACAACGACCATAGCATAGCTGACCTGTTCAACAAGTTGGGGAAAACCGTTTATTTCGACAAGGATTGCTACCTCTCCAAGCAATTCAGAGACGTGGAGGCTTATTACAACAGCCATTGGGGTACTTTCCTACGAACTTATTTTAGCGCCCCATGGTCCTTCATTTCAGTTGTCTCAGCCTGCATTCTTCTTTTGCTGGCCGCAGCTCAAACTGTTGTGTCTATCTTGAGCTATATCAAACAAATGTCGGACAAGGGAAAATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF247

Protein Analysis

523

Amino Acids

59.47

Weight (kDa)

6.14

Isoelectric Point (pI)

37.91

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF247 PF03140 50 - 501 1.9e-94 Plant protein of unknown function
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 1263
AccI GTMKAC 1 cut(s) 765
AccII CGCG 2 cut(s) 344, 407
AciI CCGC 7 cut(s) 76, 96, 342, 407, 505, 840, 1506
AclI AACGTT 1 cut(s) 963
AclWI GGATC 3 cut(s) 880, 893, 1058
AcoI YGGCCR 1 cut(s) 1503
AcuI CTGAAG 1 cut(s) 270
AcyI GRCGYC 1 cut(s) 1260
AfaI GTAC 6 cut(s) 328, 429, 594, 700, 1110, 1429
AfiI CCNNNNNNNGG 4 cut(s) 72, 514, 846, 1105
AflIII ACRYGT 1 cut(s) 986
AjiI CACGTC 1 cut(s) 1399
AjnI CCWGG 3 cut(s) 507, 595, 651
Alw21I GWGCWC 1 cut(s) 1238
Alw26I GTCTC 5 cut(s) 214, 248, 1251, 1389, 1483
AlwI GGATC 3 cut(s) 880, 893, 1058
AlwNI CAGNNNCTG 1 cut(s) 317
AoxI GGCC 3 cut(s) 197, 387, 1503
ApeKI GCWGC 3 cut(s) 62, 759, 1508
Asp700I GAANNNNTTC 2 cut(s) 279, 626
AspLEI GCGC 3 cut(s) 407, 759, 1454
AspS9I GGNCC 5 cut(s) 218, 314, 502, 517, 1461
AsuC2I CCSGG 1 cut(s) 1048
AsuHPI GGTGA 3 cut(s) 49, 218, 971
AvaII GGWCC 5 cut(s) 218, 314, 502, 517, 1461
BamHI GGATCC 1 cut(s) 885
BanII GRGCYC 1 cut(s) 1238
BauI CACGAG 1 cut(s) 170
BbsI GAAGAC 1 cut(s) 899
Bbv12I GWGCWC 1 cut(s) 1238
BbvI GCAGC 3 cut(s) 49, 746, 1520
BccI CCATC 6 cut(s) 221, 296, 398, 418, 428, 1139
BciT130I CCWGG 3 cut(s) 509, 597, 653
BclI TGATCA 2 cut(s) 382, 640
BcnI CCSGG 1 cut(s) 1048
BcoDI GTCTC 5 cut(s) 214, 248, 1251, 1389, 1483
BfaI CTAG 4 cut(s) 288, 605, 1070, 1254
BfmI CTRYAG 2 cut(s) 57, 80
BfoI RGCGCY 1 cut(s) 1455
BisI GCNGC 6 cut(s) 63, 77, 342, 760, 1506, 1509
BlsI GCNGC 6 cut(s) 64, 78, 343, 761, 1507, 1510
Bme1390I CCNGG 4 cut(s) 509, 597, 653, 1048
Bme18I GGWCC 5 cut(s) 218, 314, 502, 517, 1461
BmgBI CACGTC 1 cut(s) 1399
BmgT120I GGNCC 5 cut(s) 218, 314, 502, 517, 1461
BmiI GGNNCC 1 cut(s) 887
BmrFI CCNGG 4 cut(s) 509, 597, 653, 1048
BmsI GCATC 4 cut(s) 7, 83, 362, 1006
BpiI GAAGAC 1 cut(s) 899
BpuEI CTTGAG 3 cut(s) 509, 673, 1552
BpuMI CCSGG 1 cut(s) 1048
Bsa29I ATCGAT 2 cut(s) 42, 1092
BsaBI GATNNNNATC 1 cut(s) 21
BsaHI GRCGYC 1 cut(s) 1260
BsaI GGTCTC 2 cut(s) 214, 248
BsaJI CCNNGG 1 cut(s) 1457
Bsc4I CCNNNNNNNGG 4 cut(s) 72, 514, 846, 1105
Bse1I ACTGG 2 cut(s) 579, 1204
Bse3DI GCAATG 4 cut(s) 426, 849, 1221, 1265
Bse8I GATNNNNATC 1 cut(s) 21
BseBI CCWGG 3 cut(s) 509, 597, 653
BseCI ATCGAT 2 cut(s) 42, 1092
BseDI CCNNGG 1 cut(s) 1457
BseGI GGATG 1 cut(s) 661
BseJI GATNNNNATC 1 cut(s) 21
BseLI CCNNNNNNNGG 4 cut(s) 72, 514, 846, 1105
BseMI GCAATG 4 cut(s) 426, 849, 1221, 1265
BseMII CTCAG 2 cut(s) 583, 1494
BseNI ACTGG 2 cut(s) 579, 1204
BseRI GAGGAG 1 cut(s) 512
BseXI GCAGC 3 cut(s) 49, 746, 1520
BseYI CCCAGC 1 cut(s) 832
BsgI GTGCAG 1 cut(s) 330
Bsh1236I CGCG 2 cut(s) 344, 407
Bsh1285I CGRYCG 1 cut(s) 840
BshFI GGCC 3 cut(s) 199, 389, 1505
BshVI ATCGAT 2 cut(s) 42, 1092
BsiEI CGRYCG 1 cut(s) 840
BsiHKAI GWGCWC 1 cut(s) 1238
BsiSI CCGG 1 cut(s) 1047
BslI CCNNNNNNNGG 4 cut(s) 72, 514, 846, 1105
BsmAI GTCTC 5 cut(s) 214, 248, 1251, 1389, 1483
BsmBI CGTCTC 2 cut(s) 1251, 1389
BsmI GAATGC 3 cut(s) 759, 893, 1488
BsnI GGCC 3 cut(s) 199, 389, 1505
Bso31I GGTCTC 2 cut(s) 214, 248
Bsp1286I GDGCHC 1 cut(s) 1238
Bsp143I GATC 7 cut(s) 22, 39, 319, 382, 640, 885, 1050
Bsp19I CCATGG 1 cut(s) 1457
BspACI CCGC 7 cut(s) 76, 96, 342, 407, 505, 840, 1506
BspANI GGCC 3 cut(s) 199, 389, 1505
BspCNI CTCAG 2 cut(s) 582, 1493
BspDI ATCGAT 2 cut(s) 42, 1092
BspFNI CGCG 2 cut(s) 344, 407
BspHI TCATGA 1 cut(s) 1230
BspLI GGNNCC 1 cut(s) 887
BspPI GGATC 3 cut(s) 880, 893, 1058
BspTNI GGTCTC 2 cut(s) 214, 248
BsrDI GCAATG 4 cut(s) 426, 849, 1221, 1265
BsrI ACTGG 2 cut(s) 579, 1204
BssECI CCNNGG 1 cut(s) 1457
BssMI GATC 7 cut(s) 22, 39, 319, 382, 640, 885, 1050
BssNI GRCGYC 1 cut(s) 1260
BssSI CACGAG 1 cut(s) 170
BssT1I CCWWGG 1 cut(s) 1457
Bst2BI CACGAG 1 cut(s) 170
Bst2UI CCWGG 3 cut(s) 509, 597, 653
Bst4CI ACNGT 2 cut(s) 1354, 1519
Bst6I CTCTTC 2 cut(s) 246, 489
BstACI GRCGYC 1 cut(s) 1260
BstC8I GCNNGC 3 cut(s) 615, 1486, 1503
BstDEI CTNAG 5 cut(s) 323, 569, 911, 1037, 1480
BstDSI CCRYGG 1 cut(s) 1457
BstF5I GGATG 1 cut(s) 661
BstFNI CGCG 2 cut(s) 344, 407
BstH2I RGCGCY 1 cut(s) 1455
BstHHI GCGC 3 cut(s) 407, 759, 1454
BstKTI GATC 7 cut(s) 25, 42, 322, 385, 643, 888, 1053
BstMAI GTCTC 5 cut(s) 214, 248, 1251, 1389, 1483
BstMBI GATC 7 cut(s) 22, 39, 319, 382, 640, 885, 1050
BstMCI CGRYCG 1 cut(s) 840
BstMWI GCNNNNNNNGC 2 cut(s) 76, 614
BstNI CCWGG 3 cut(s) 509, 597, 653
BstNSI RCATGY 2 cut(s) 816, 990
BstSCI CCNGG 4 cut(s) 507, 595, 651, 1046
BstSFI CTRYAG 2 cut(s) 57, 80
BstUI CGCG 2 cut(s) 344, 407
BstV1I GCAGC 3 cut(s) 49, 746, 1520
BstV2I GAAGAC 1 cut(s) 899
BstX2I RGATCY 1 cut(s) 885
BstYI RGATCY 1 cut(s) 885
Bsu15I ATCGAT 2 cut(s) 42, 1092
BsuRI GGCC 3 cut(s) 199, 389, 1505
BsuTUI ATCGAT 2 cut(s) 42, 1092
BtgI CCRYGG 1 cut(s) 1457
BtgZI GCGATG 1 cut(s) 376
BtrI CACGTC 1 cut(s) 1399
BtsCI GGATG 1 cut(s) 661
BtsIMutI CAGTG 1 cut(s) 586
Cac8I GCNNGC 3 cut(s) 615, 1486, 1503
CaiI CAGNNNCTG 1 cut(s) 317
CciI TCATGA 1 cut(s) 1230
CfoI GCGC 3 cut(s) 407, 759, 1454
Cfr13I GGNCC 5 cut(s) 218, 314, 502, 517, 1461
ClaI ATCGAT 2 cut(s) 42, 1092
CsiI ACCWGGT 1 cut(s) 595
Csp6I GTAC 6 cut(s) 327, 428, 593, 699, 1109, 1428
CspCI CAANNNNNGTGG 2 cut(s) 1204, 1239
CviAII CATG 5 cut(s) 67, 813, 987, 1231, 1458
CviQI GTAC 6 cut(s) 327, 428, 593, 699, 1109, 1428
DdeI CTNAG 5 cut(s) 323, 569, 911, 1037, 1480
DpnI GATC 7 cut(s) 24, 41, 321, 384, 642, 887, 1052
DpnII GATC 7 cut(s) 22, 39, 319, 382, 640, 885, 1050
DraI TTTAAA 1 cut(s) 337
EaeI YGGCCR 1 cut(s) 1503
Eam1104I CTCTTC 2 cut(s) 246, 489
EarI CTCTTC 2 cut(s) 246, 489
Ecl136II GAGCTC 1 cut(s) 1236
Eco130I CCWWGG 1 cut(s) 1457
Eco147I AGGCCT 1 cut(s) 199
Eco24I GRGCYC 1 cut(s) 1238
Eco31I GGTCTC 2 cut(s) 214, 248
Eco32I GATATC 1 cut(s) 734
Eco47I GGWCC 5 cut(s) 218, 314, 502, 517, 1461
Eco53kI GAGCTC 1 cut(s) 1236
Eco57I CTGAAG 1 cut(s) 270
EcoICRI GAGCTC 1 cut(s) 1236
EcoO109I RGGNCCY 1 cut(s) 314
EcoRII CCWGG 3 cut(s) 507, 595, 651
EcoRV GATATC 1 cut(s) 734
EcoT14I CCWWGG 1 cut(s) 1457
EcoT38I GRGCYC 1 cut(s) 1238
ErhI CCWWGG 1 cut(s) 1457
Esp3I CGTCTC 2 cut(s) 1251, 1389
FaeI CATG 5 cut(s) 70, 816, 990, 1234, 1461
FatI CATG 5 cut(s) 66, 812, 986, 1230, 1457
FauNDI CATATG 1 cut(s) 802
FbaI TGATCA 2 cut(s) 382, 640
FblI GTMKAC 1 cut(s) 765
Fnu4HI GCNGC 6 cut(s) 63, 77, 342, 760, 1506, 1509
FokI GGATG 1 cut(s) 668
FriOI GRGCYC 1 cut(s) 1238
Fsp4HI GCNGC 6 cut(s) 63, 77, 342, 760, 1506, 1509
FspBI CTAG 4 cut(s) 288, 605, 1070, 1254
GlaI GCGC 3 cut(s) 406, 758, 1453
GluI GCNGC 6 cut(s) 63, 77, 342, 760, 1506, 1509
GsaI CCCAGC 1 cut(s) 836
HaeII RGCGCY 1 cut(s) 1455
HaeIII GGCC 3 cut(s) 199, 389, 1505
HapII CCGG 1 cut(s) 1047
HhaI GCGC 3 cut(s) 407, 759, 1454
Hin1I GRCGYC 1 cut(s) 1260
Hin1II CATG 5 cut(s) 70, 816, 990, 1234, 1461
Hin6I GCGC 3 cut(s) 405, 757, 1452
HinP1I GCGC 3 cut(s) 405, 757, 1452
HindIII AAGCTT 4 cut(s) 276, 527, 691, 1078
HinfI GANTC 7 cut(s) 53, 110, 177, 245, 584, 972, 1013
HpaII CCGG 1 cut(s) 1047
HphI GGTGA 3 cut(s) 49, 218, 971
Hpy166II GTNNAC 1 cut(s) 766
Hpy188I TCNGA 6 cut(s) 105, 218, 250, 1012, 1394, 1555
Hpy188III TCNNGA 6 cut(s) 26, 161, 288, 992, 1231, 1531
Hpy8I GTNNAC 1 cut(s) 766
Hpy99I CGWCG 1 cut(s) 1265
HpyAV CCTTC 4 cut(s) 493, 986, 1237, 1474
HpyCH4III ACNGT 2 cut(s) 1354, 1519
HpyCH4IV ACGT 4 cut(s) 954, 963, 1260, 1398
HpyCH4V TGCA 9 cut(s) 70, 147, 311, 419, 800, 860, 893, 1114, 1488
HpyF10VI GCNNNNNNNGC 2 cut(s) 76, 614
HpyF3I CTNAG 5 cut(s) 323, 569, 911, 1037, 1480
HpySE526I ACGT 4 cut(s) 954, 963, 1260, 1398
Hsp92I GRCGYC 1 cut(s) 1260
Hsp92II CATG 5 cut(s) 70, 816, 990, 1234, 1461
HspAI GCGC 3 cut(s) 405, 757, 1452
Ksp22I TGATCA 2 cut(s) 382, 640
Kzo9I GATC 7 cut(s) 22, 39, 319, 382, 640, 885, 1050
LmnI GCTCC 1 cut(s) 994
Lsp1109I GCAGC 3 cut(s) 49, 746, 1520
LweI GCATC 4 cut(s) 7, 83, 362, 1006
MabI ACCWGGT 1 cut(s) 595
MaeI CTAG 4 cut(s) 288, 605, 1070, 1254
MaeII ACGT 4 cut(s) 954, 963, 1260, 1398
MaeIII GTNAC 4 cut(s) 672, 805, 1141, 1277
MalI GATC 7 cut(s) 24, 41, 321, 384, 642, 887, 1052
MboI GATC 7 cut(s) 22, 39, 319, 382, 640, 885, 1050
MboII GAAGA 8 cut(s) 263, 381, 506, 619, 899, 928, 1147, 1484
MflI RGATCY 1 cut(s) 885
MhlI GDGCHC 1 cut(s) 1238
MluCI AATT 1 cut(s) 1388
MmeI TCCRAC 2 cut(s) 83, 1533
MnlI CCTC 9 cut(s) 179, 349, 466, 530, 533, 840, 1054, 1388, 1396
MroXI GAANNNNTTC 2 cut(s) 279, 626
MseI TTAA 3 cut(s) 336, 663, 1085
MslI CAYNNNNRTG 1 cut(s) 395
MspI CCGG 1 cut(s) 1047
MspR9I CCNGG 4 cut(s) 509, 597, 653, 1048
Mva1269I GAATGC 3 cut(s) 759, 893, 1488
MvaI CCWGG 3 cut(s) 509, 597, 653
MvnI CGCG 2 cut(s) 344, 407
MwoI GCNNNNNNNGC 2 cut(s) 76, 614
NciI CCSGG 1 cut(s) 1048
NcoI CCATGG 1 cut(s) 1457
NdeI CATATG 1 cut(s) 802
NdeII GATC 7 cut(s) 22, 39, 319, 382, 640, 885, 1050
NlaIII CATG 5 cut(s) 70, 816, 990, 1234, 1461
NlaIV GGNNCC 1 cut(s) 887
NmuCI GTSAC 1 cut(s) 1141
NspI RCATGY 2 cut(s) 816, 990
PagI TCATGA 1 cut(s) 1230
PceI AGGCCT 1 cut(s) 199
PciI ACATGT 1 cut(s) 986
PcsI WCGNNNNNNNCGW 2 cut(s) 48, 960
PctI GAATGC 3 cut(s) 759, 893, 1488
PdmI GAANNNNTTC 2 cut(s) 279, 626
PfeI GAWTC 7 cut(s) 53, 110, 177, 245, 584, 972, 1013
PfoI TCCNGGA 2 cut(s) 651, 1046
PkrI GCNGC 6 cut(s) 64, 78, 343, 761, 1507, 1510
PpuMI RGGWCCY 1 cut(s) 314
PscI ACATGT 1 cut(s) 986
Psp124BI GAGCTC 1 cut(s) 1238
Psp1406I AACGTT 1 cut(s) 963
Psp5II RGGWCCY 1 cut(s) 314
Psp6I CCWGG 3 cut(s) 507, 595, 651
PspFI CCCAGC 1 cut(s) 832
PspGI CCWGG 3 cut(s) 507, 595, 651
PspN4I GGNNCC 1 cut(s) 887
PspPI GGNCC 5 cut(s) 218, 314, 502, 517, 1461
PspPPI RGGWCCY 1 cut(s) 314
PstNI CAGNNNCTG 1 cut(s) 317
PsuI RGATCY 1 cut(s) 885
RsaI GTAC 6 cut(s) 328, 429, 594, 700, 1110, 1429
RsaNI GTAC 6 cut(s) 327, 428, 593, 699, 1109, 1428
RseI CAYNNNNRTG 1 cut(s) 395
SacI GAGCTC 1 cut(s) 1238
SaqAI TTAA 3 cut(s) 336, 663, 1085
SatI GCNGC 6 cut(s) 63, 77, 342, 760, 1506, 1509
Sau3AI GATC 7 cut(s) 22, 39, 319, 382, 640, 885, 1050
Sau96I GGNCC 5 cut(s) 218, 314, 502, 517, 1461
ScrFI CCNGG 4 cut(s) 509, 597, 653, 1048
SduI GDGCHC 1 cut(s) 1238
SexAI ACCWGGT 1 cut(s) 595
SfaNI GCATC 4 cut(s) 7, 83, 362, 1006
SfcI CTRYAG 2 cut(s) 57, 80
SinI GGWCC 5 cut(s) 218, 314, 502, 517, 1461
SmiMI CAYNNNNRTG 1 cut(s) 395
SmlI CTYRAG 3 cut(s) 524, 688, 1531
SmoI CTYRAG 3 cut(s) 524, 688, 1531
Sse9I AATT 1 cut(s) 1388
SseBI AGGCCT 1 cut(s) 199
SsiI CCGC 7 cut(s) 76, 96, 342, 407, 505, 840, 1506
SspI AATATT 1 cut(s) 748
SspMI CTAG 4 cut(s) 288, 605, 1070, 1254
SstI GAGCTC 1 cut(s) 1238
StuI AGGCCT 1 cut(s) 199
StyD4I CCNGG 4 cut(s) 507, 595, 651, 1046
StyI CCWWGG 1 cut(s) 1457
TaaI ACNGT 2 cut(s) 1354, 1519
TaiI ACGT 4 cut(s) 957, 966, 1263, 1401
TaqI TCGA 5 cut(s) 42, 730, 825, 1092, 1362
TasI AATT 1 cut(s) 1388
TauI GCSGC 3 cut(s) 79, 344, 1508
TfiI GAWTC 7 cut(s) 53, 110, 177, 245, 584, 972, 1013
Tru1I TTAA 3 cut(s) 336, 663, 1085
Tru9I TTAA 3 cut(s) 336, 663, 1085
TscAI CASTG 1 cut(s) 586
TseFI GTSAC 1 cut(s) 1141
TseI GCWGC 3 cut(s) 62, 759, 1508
Tsp45I GTSAC 1 cut(s) 1141
TspDTI ATGAA 5 cut(s) 278, 576, 1147, 1219, 1456
TspGWI ACGGA 1 cut(s) 686
TspRI CASTG 1 cut(s) 586
VpaK11BI GGWCC 5 cut(s) 218, 314, 502, 517, 1461
XbaI TCTAGA 1 cut(s) 287
XceI RCATGY 2 cut(s) 816, 990
XmiI GTMKAC 1 cut(s) 765
XmnI GAANNNNTTC 2 cut(s) 279, 626
XspI CTAG 4 cut(s) 288, 605, 1070, 1254
ZraI GACGTC 1 cut(s) 1261
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.