Rroxscaffold_6G00408790

UPF0481 protein At3g47200-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000006
OG0002694
Physical Location & Seq
Reverse (-)
31287223 .. 31288809
1587 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_6G00408790.1

Sequence Viewer

Length: 1587 bp
ATGGAGAATGGTATCACAATGGTAGAGCTAACTGATGCCGATCAGGAACGAGTGGTGATCGATATTGACGAATCTGTAGCTGCCATGCAAAGGCGCCTACAGGAAAGGAGATGCGGAAGTCGGAGAGCATCAATATATATGGACAACTTTAGTTCAGTTGTCTGCATGTACAGAGTTCCTGAAAGCCTCGTGGGAATTAGTCCAAAAGCTATTAGGCCTGAAAAGGTCTCCATCGGACCTTATCACCACAGCAAGAGCAAGGAATCTGAAGAGACCAAAATGAAGTATTTTGAAGCTTTTCTCTCTAGAAAAAAGAGAGATGGTAGTGCTGGACCTGATCTAAGTACCTTGTTTAAAGCCGCGAGAAATATGGAGGCTGAAGCAAGAAGATGCTATGACGATCAGGCCATCGCTATGTCAAGCGCGGAGTTTGTTGCAATGATGGTACTTGATGGGTGCTTTATCATTGAGCTTTTCCTACAAGACCAAGAGGACAAACTCAAAGATGTTGAAGAGAAGGACCGCCTGGTTATGGACCTCCTCAAGCTTGAAAACCAATTGCCTTTCTTCGTCCTTGAAAGACTATTCAGCCAGTCCAGTGATTCATCTGTACCAGGTTCTCTAGCCTTGCTTGCCTTGAAGTTCTTCAATGTTTATGATCACAAAGTCCTGGATGATGTTAAACACTTGTTACACTTGTTTCACTCAAGCTTCCGTACCCCTATATTACTAGAACGAGATAATACTCGATATCAGCTCTCTAATATTAGAATGCGCTGCGTAGACTTACTTGGGGAAGCTGATGATGTAATGATTGCATATGTAACGACATGCCTATCAATCGACAGCCCAGCGACCGCAATGAGGTGCTTCCTTGCAACCCCTTTTCTATTTGTTTGGGGGATCCTTGCATTCCTTTATCGTCTTCTAAGGTTAGTCGGTTCAATCCTTTTCTTCTTTCTTTGGTGGAACGTGGATAACGTTCAAAGATTCCTTCACCCCACAGGTCAGGAGCATCGTCCTTTCTCCGATTCAATCCAATGTGCCACACAACTAAGACCCTCCGGGATCATTTTCAAGCCACTCCTAGCAAAAAGCTTCTTAACTATCGATTACCACAAACGGGTACTGCAAATACCACCCATAACCATCAACGATGTCTCTATTACTATCTTCATCAACAGCATAGCCTTAGAACAATGTTGCCCACACAAGACTGGTTCTTTATTTACCACTTACATTGCCTTCATGAGCTCTCTCATCAATACGCCTAGAGACGTCGCACTGCTTTCTGGTAACGGGATTATCATTACCAGCTTCTCACACAACGACCATAGCATTGCTGACCAGTTCAACAAGTTGGGGAAAACAGTTTATTTCGACAAGGATTGCTACCTCTCCAAGCAATTCAGAGACGTGGAGGCTTATTACAACAGCCATTGGGGTACTTTTCTACGAACTTATTTCAGCGCCCTATGGTCCTTCATTTCAGTTGTCTCAGCCTGCATTCTTCTTTTGCTGGCCGCAGCTCAAACTATTGTGTCTATCTTGAGCTATATCAAACAAATGTCAGACAAGGGAAAATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF247

Protein Analysis

528

Amino Acids

60.27

Weight (kDa)

6.68

Isoelectric Point (pI)

40.97

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF247 PF03140 57 - 507 1.2e-91 Plant protein of unknown function
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 1281
AccB1I GGYRCC 1 cut(s) 93
AccI GTMKAC 1 cut(s) 783
AccII CGCG 2 cut(s) 362, 425
AciI CCGC 6 cut(s) 114, 360, 425, 523, 858, 1524
AclI AACGTT 1 cut(s) 981
AclWI GGATC 3 cut(s) 898, 911, 1076
AcoI YGGCCR 1 cut(s) 1521
AcuI CTGAAG 2 cut(s) 288, 399
AcyI GRCGYC 2 cut(s) 94, 1278
AfaI GTAC 7 cut(s) 170, 346, 447, 612, 718, 1128, 1447
AfiI CCNNNNNNNGG 4 cut(s) 90, 532, 864, 1123
AjiI CACGTC 1 cut(s) 1417
AjnI CCWGG 3 cut(s) 525, 613, 669
Alw21I GWGCWC 1 cut(s) 1256
Alw26I GTCTC 6 cut(s) 232, 266, 1165, 1269, 1407, 1501
AlwI GGATC 3 cut(s) 898, 911, 1076
AoxI GGCC 3 cut(s) 215, 405, 1521
ApeKI GCWGC 3 cut(s) 80, 777, 1526
Asp700I GAANNNNTTC 2 cut(s) 297, 644
AspLEI GCGC 4 cut(s) 96, 425, 777, 1472
AspS9I GGNCC 5 cut(s) 236, 332, 520, 535, 1479
AsuC2I CCSGG 1 cut(s) 1066
AsuHPI GGTGA 3 cut(s) 67, 236, 989
AvaII GGWCC 5 cut(s) 236, 332, 520, 535, 1479
BamHI GGATCC 1 cut(s) 903
BanI GGYRCC 1 cut(s) 93
BanII GRGCYC 1 cut(s) 1256
BauI CACGAG 1 cut(s) 188
BbsI GAAGAC 1 cut(s) 917
Bbv12I GWGCWC 1 cut(s) 1256
BbvI GCAGC 3 cut(s) 67, 764, 1538
BccI CCATC 6 cut(s) 239, 314, 416, 436, 446, 1157
BciT130I CCWGG 3 cut(s) 527, 615, 671
BclI TGATCA 1 cut(s) 658
BcnI CCSGG 1 cut(s) 1066
BcoDI GTCTC 6 cut(s) 232, 266, 1165, 1269, 1407, 1501
BfaI CTAG 5 cut(s) 306, 623, 731, 1088, 1272
BfmI CTRYAG 2 cut(s) 75, 98
BfoI RGCGCY 2 cut(s) 97, 1473
BisI GCNGC 5 cut(s) 81, 360, 778, 1524, 1527
BlsI GCNGC 5 cut(s) 82, 361, 779, 1525, 1528
Bme1390I CCNGG 4 cut(s) 527, 615, 671, 1066
Bme18I GGWCC 5 cut(s) 236, 332, 520, 535, 1479
BmgBI CACGTC 1 cut(s) 1417
BmgT120I GGNCC 5 cut(s) 236, 332, 520, 535, 1479
BmiI GGNNCC 2 cut(s) 95, 905
BmrFI CCNGG 4 cut(s) 527, 615, 671, 1066
BmsI GCATC 5 cut(s) 25, 101, 137, 380, 1024
BpiI GAAGAC 1 cut(s) 917
BpuEI CTTGAG 3 cut(s) 527, 691, 1570
BpuMI CCSGG 1 cut(s) 1066
Bsa29I ATCGAT 2 cut(s) 60, 1110
BsaBI GATNNNNATC 1 cut(s) 39
BsaHI GRCGYC 2 cut(s) 94, 1278
BsaI GGTCTC 2 cut(s) 232, 266
Bsc4I CCNNNNNNNGG 4 cut(s) 90, 532, 864, 1123
Bse1I ACTGG 4 cut(s) 592, 597, 1222, 1348
Bse3DI GCAATG 4 cut(s) 444, 867, 1239, 1338
Bse8I GATNNNNATC 1 cut(s) 39
BseBI CCWGG 3 cut(s) 527, 615, 671
BseCI ATCGAT 2 cut(s) 60, 1110
BseGI GGATG 1 cut(s) 679
BseJI GATNNNNATC 1 cut(s) 39
BseLI CCNNNNNNNGG 4 cut(s) 90, 532, 864, 1123
BseMI GCAATG 4 cut(s) 444, 867, 1239, 1338
BseMII CTCAG 1 cut(s) 1512
BseNI ACTGG 4 cut(s) 592, 597, 1222, 1348
BseRI GAGGAG 1 cut(s) 530
BseXI GCAGC 3 cut(s) 67, 764, 1538
BseYI CCCAGC 1 cut(s) 850
Bsh1236I CGCG 2 cut(s) 362, 425
Bsh1285I CGRYCG 1 cut(s) 858
BshFI GGCC 3 cut(s) 217, 407, 1523
BshNI GGYRCC 1 cut(s) 93
BshVI ATCGAT 2 cut(s) 60, 1110
BsiEI CGRYCG 1 cut(s) 858
BsiHKAI GWGCWC 1 cut(s) 1256
BsiSI CCGG 1 cut(s) 1065
BslI CCNNNNNNNGG 4 cut(s) 90, 532, 864, 1123
BsmAI GTCTC 6 cut(s) 232, 266, 1165, 1269, 1407, 1501
BsmBI CGTCTC 2 cut(s) 1269, 1407
BsmI GAATGC 3 cut(s) 777, 911, 1506
BsnI GGCC 3 cut(s) 217, 407, 1523
Bso31I GGTCTC 2 cut(s) 232, 266
Bsp1286I GDGCHC 1 cut(s) 1256
Bsp1407I TGTACA 1 cut(s) 168
Bsp143I GATC 7 cut(s) 40, 57, 337, 400, 658, 903, 1068
BspACI CCGC 6 cut(s) 114, 360, 425, 523, 858, 1524
BspANI GGCC 3 cut(s) 217, 407, 1523
BspCNI CTCAG 1 cut(s) 1511
BspDI ATCGAT 2 cut(s) 60, 1110
BspFNI CGCG 2 cut(s) 362, 425
BspHI TCATGA 1 cut(s) 1248
BspLI GGNNCC 2 cut(s) 95, 905
BspPI GGATC 3 cut(s) 898, 911, 1076
BspT107I GGYRCC 1 cut(s) 93
BspTNI GGTCTC 2 cut(s) 232, 266
BsrDI GCAATG 4 cut(s) 444, 867, 1239, 1338
BsrGI TGTACA 1 cut(s) 168
BsrI ACTGG 4 cut(s) 592, 597, 1222, 1348
BssMI GATC 7 cut(s) 40, 57, 337, 400, 658, 903, 1068
BssNI GRCGYC 2 cut(s) 94, 1278
BssSI CACGAG 1 cut(s) 188
Bst2BI CACGAG 1 cut(s) 188
Bst2UI CCWGG 3 cut(s) 527, 615, 671
Bst4CI ACNGT 1 cut(s) 1372
Bst6I CTCTTC 2 cut(s) 264, 507
BstACI GRCGYC 2 cut(s) 94, 1278
BstAUI TGTACA 1 cut(s) 168
BstC8I GCNNGC 3 cut(s) 633, 1504, 1521
BstDEI CTNAG 5 cut(s) 341, 929, 1055, 1192, 1498
BstF5I GGATG 1 cut(s) 679
BstFNI CGCG 2 cut(s) 362, 425
BstH2I RGCGCY 2 cut(s) 97, 1473
BstHHI GCGC 4 cut(s) 96, 425, 777, 1472
BstKTI GATC 7 cut(s) 43, 60, 340, 403, 661, 906, 1071
BstMAI GTCTC 6 cut(s) 232, 266, 1165, 1269, 1407, 1501
BstMBI GATC 7 cut(s) 40, 57, 337, 400, 658, 903, 1068
BstMCI CGRYCG 1 cut(s) 858
BstMWI GCNNNNNNNGC 1 cut(s) 632
BstNI CCWGG 3 cut(s) 527, 615, 671
BstNSI RCATGY 2 cut(s) 169, 834
BstSCI CCNGG 4 cut(s) 525, 613, 669, 1064
BstSFI CTRYAG 2 cut(s) 75, 98
BstUI CGCG 2 cut(s) 362, 425
BstV1I GCAGC 3 cut(s) 67, 764, 1538
BstV2I GAAGAC 1 cut(s) 917
BstX2I RGATCY 1 cut(s) 903
BstYI RGATCY 1 cut(s) 903
Bsu15I ATCGAT 2 cut(s) 60, 1110
BsuRI GGCC 3 cut(s) 217, 407, 1523
BsuTUI ATCGAT 2 cut(s) 60, 1110
BtgZI GCGATG 1 cut(s) 394
BtrI CACGTC 1 cut(s) 1417
BtsCI GGATG 1 cut(s) 679
BtsI GCAGTG 1 cut(s) 1283
BtsIMutI CAGTG 2 cut(s) 604, 1283
Cac8I GCNNGC 3 cut(s) 633, 1504, 1521
CciI TCATGA 1 cut(s) 1248
CfoI GCGC 4 cut(s) 96, 425, 777, 1472
Cfr13I GGNCC 5 cut(s) 236, 332, 520, 535, 1479
ClaI ATCGAT 2 cut(s) 60, 1110
CsiI ACCWGGT 1 cut(s) 613
Csp6I GTAC 7 cut(s) 169, 345, 446, 611, 717, 1127, 1446
CspCI CAANNNNNGTGG 2 cut(s) 1222, 1257
CviAII CATG 4 cut(s) 85, 166, 831, 1249
CviQI GTAC 7 cut(s) 169, 345, 446, 611, 717, 1127, 1446
DdeI CTNAG 5 cut(s) 341, 929, 1055, 1192, 1498
DinI GGCGCC 1 cut(s) 95
DpnI GATC 7 cut(s) 42, 59, 339, 402, 660, 905, 1070
DpnII GATC 7 cut(s) 40, 57, 337, 400, 658, 903, 1068
DraI TTTAAA 1 cut(s) 355
EaeI YGGCCR 1 cut(s) 1521
Eam1104I CTCTTC 2 cut(s) 264, 507
EarI CTCTTC 2 cut(s) 264, 507
Ecl136II GAGCTC 1 cut(s) 1254
Eco147I AGGCCT 1 cut(s) 217
Eco24I GRGCYC 1 cut(s) 1256
Eco31I GGTCTC 2 cut(s) 232, 266
Eco32I GATATC 1 cut(s) 752
Eco47I GGWCC 5 cut(s) 236, 332, 520, 535, 1479
Eco53kI GAGCTC 1 cut(s) 1254
Eco57I CTGAAG 2 cut(s) 288, 399
EcoICRI GAGCTC 1 cut(s) 1254
EcoRII CCWGG 3 cut(s) 525, 613, 669
EcoRV GATATC 1 cut(s) 752
EcoT38I GRGCYC 1 cut(s) 1256
EgeI GGCGCC 1 cut(s) 95
EheI GGCGCC 1 cut(s) 95
Esp3I CGTCTC 2 cut(s) 1269, 1407
FaeI CATG 4 cut(s) 88, 169, 834, 1252
FatI CATG 4 cut(s) 84, 165, 830, 1248
FauNDI CATATG 1 cut(s) 820
FbaI TGATCA 1 cut(s) 658
FblI GTMKAC 1 cut(s) 783
Fnu4HI GCNGC 5 cut(s) 81, 360, 778, 1524, 1527
FokI GGATG 1 cut(s) 686
FriOI GRGCYC 1 cut(s) 1256
Fsp4HI GCNGC 5 cut(s) 81, 360, 778, 1524, 1527
FspBI CTAG 5 cut(s) 306, 623, 731, 1088, 1272
GlaI GCGC 4 cut(s) 95, 424, 776, 1471
GluI GCNGC 5 cut(s) 81, 360, 778, 1524, 1527
GsaI CCCAGC 1 cut(s) 854
HaeII RGCGCY 2 cut(s) 97, 1473
HaeIII GGCC 3 cut(s) 217, 407, 1523
HapII CCGG 1 cut(s) 1065
HhaI GCGC 4 cut(s) 96, 425, 777, 1472
Hin1I GRCGYC 2 cut(s) 94, 1278
Hin1II CATG 4 cut(s) 88, 169, 834, 1252
Hin6I GCGC 4 cut(s) 94, 423, 775, 1470
HinP1I GCGC 4 cut(s) 94, 423, 775, 1470
HindIII AAGCTT 4 cut(s) 294, 545, 709, 1096
HinfI GANTC 5 cut(s) 71, 263, 602, 990, 1031
HpaII CCGG 1 cut(s) 1065
HphI GGTGA 3 cut(s) 67, 236, 989
Hpy166II GTNNAC 1 cut(s) 784
Hpy188I TCNGA 6 cut(s) 123, 236, 268, 1030, 1412, 1573
Hpy188III TCNNGA 6 cut(s) 44, 179, 306, 1010, 1249, 1549
Hpy8I GTNNAC 1 cut(s) 784
Hpy99I CGWCG 1 cut(s) 1283
HpyAV CCTTC 4 cut(s) 511, 1004, 1255, 1492
HpyCH4III ACNGT 1 cut(s) 1372
HpyCH4IV ACGT 4 cut(s) 972, 981, 1278, 1416
HpyCH4V TGCA 8 cut(s) 88, 165, 437, 818, 878, 911, 1132, 1506
HpyF10VI GCNNNNNNNGC 1 cut(s) 632
HpyF3I CTNAG 5 cut(s) 341, 929, 1055, 1192, 1498
HpySE526I ACGT 4 cut(s) 972, 981, 1278, 1416
Hsp92I GRCGYC 2 cut(s) 94, 1278
Hsp92II CATG 4 cut(s) 88, 169, 834, 1252
HspAI GCGC 4 cut(s) 94, 423, 775, 1470
KasI GGCGCC 1 cut(s) 93
Ksp22I TGATCA 1 cut(s) 658
Kzo9I GATC 7 cut(s) 40, 57, 337, 400, 658, 903, 1068
LmnI GCTCC 1 cut(s) 1012
Lsp1109I GCAGC 3 cut(s) 67, 764, 1538
LweI GCATC 5 cut(s) 25, 101, 137, 380, 1024
MabI ACCWGGT 1 cut(s) 613
MaeI CTAG 5 cut(s) 306, 623, 731, 1088, 1272
MaeII ACGT 4 cut(s) 972, 981, 1278, 1416
MaeIII GTNAC 3 cut(s) 690, 823, 1295
MalI GATC 7 cut(s) 42, 59, 339, 402, 660, 905, 1070
MboI GATC 7 cut(s) 40, 57, 337, 400, 658, 903, 1068
MboII GAAGA 9 cut(s) 281, 399, 524, 559, 637, 917, 946, 1165, 1502
MfeI CAATTG 1 cut(s) 557
MflI RGATCY 1 cut(s) 903
MhlI GDGCHC 1 cut(s) 1256
MluCI AATT 3 cut(s) 195, 557, 1406
Mly113I GGCGCC 1 cut(s) 94
MmeI TCCRAC 1 cut(s) 101
MnlI CCTC 9 cut(s) 197, 367, 484, 548, 551, 858, 1072, 1406, 1414
MroXI GAANNNNTTC 2 cut(s) 297, 644
MseI TTAA 3 cut(s) 354, 681, 1103
MslI CAYNNNNRTG 1 cut(s) 413
MspI CCGG 1 cut(s) 1065
MspR9I CCNGG 4 cut(s) 527, 615, 671, 1066
MunI CAATTG 1 cut(s) 557
Mva1269I GAATGC 3 cut(s) 777, 911, 1506
MvaI CCWGG 3 cut(s) 527, 615, 671
MvnI CGCG 2 cut(s) 362, 425
MwoI GCNNNNNNNGC 1 cut(s) 632
NarI GGCGCC 1 cut(s) 94
NciI CCSGG 1 cut(s) 1066
NdeI CATATG 1 cut(s) 820
NdeII GATC 7 cut(s) 40, 57, 337, 400, 658, 903, 1068
NlaIII CATG 4 cut(s) 88, 169, 834, 1252
NlaIV GGNNCC 2 cut(s) 95, 905
NspI RCATGY 2 cut(s) 169, 834
PagI TCATGA 1 cut(s) 1248
PceI AGGCCT 1 cut(s) 217
PcsI WCGNNNNNNNCGW 2 cut(s) 66, 978
PctI GAATGC 3 cut(s) 777, 911, 1506
PdmI GAANNNNTTC 2 cut(s) 297, 644
PfeI GAWTC 5 cut(s) 71, 263, 602, 990, 1031
PfoI TCCNGGA 2 cut(s) 669, 1064
PkrI GCNGC 5 cut(s) 82, 361, 779, 1525, 1528
PluTI GGCGCC 1 cut(s) 97
Psp124BI GAGCTC 1 cut(s) 1256
Psp1406I AACGTT 1 cut(s) 981
Psp6I CCWGG 3 cut(s) 525, 613, 669
PspFI CCCAGC 1 cut(s) 850
PspGI CCWGG 3 cut(s) 525, 613, 669
PspN4I GGNNCC 2 cut(s) 95, 905
PspPI GGNCC 5 cut(s) 236, 332, 520, 535, 1479
PsuI RGATCY 1 cut(s) 903
RsaI GTAC 7 cut(s) 170, 346, 447, 612, 718, 1128, 1447
RsaNI GTAC 7 cut(s) 169, 345, 446, 611, 717, 1127, 1446
RseI CAYNNNNRTG 1 cut(s) 413
SacI GAGCTC 1 cut(s) 1256
SaqAI TTAA 3 cut(s) 354, 681, 1103
SatI GCNGC 5 cut(s) 81, 360, 778, 1524, 1527
Sau3AI GATC 7 cut(s) 40, 57, 337, 400, 658, 903, 1068
Sau96I GGNCC 5 cut(s) 236, 332, 520, 535, 1479
ScrFI CCNGG 4 cut(s) 527, 615, 671, 1066
SduI GDGCHC 1 cut(s) 1256
SexAI ACCWGGT 1 cut(s) 613
SfaNI GCATC 5 cut(s) 25, 101, 137, 380, 1024
SfcI CTRYAG 2 cut(s) 75, 98
SfoI GGCGCC 1 cut(s) 95
SinI GGWCC 5 cut(s) 236, 332, 520, 535, 1479
SmiMI CAYNNNNRTG 1 cut(s) 413
SmlI CTYRAG 3 cut(s) 542, 706, 1549
SmoI CTYRAG 3 cut(s) 542, 706, 1549
Sse9I AATT 3 cut(s) 195, 557, 1406
SseBI AGGCCT 1 cut(s) 217
SsiI CCGC 6 cut(s) 114, 360, 425, 523, 858, 1524
SspDI GGCGCC 1 cut(s) 93
SspI AATATT 1 cut(s) 766
SspMI CTAG 5 cut(s) 306, 623, 731, 1088, 1272
SstI GAGCTC 1 cut(s) 1256
StuI AGGCCT 1 cut(s) 217
StyD4I CCNGG 4 cut(s) 525, 613, 669, 1064
TaaI ACNGT 1 cut(s) 1372
TaiI ACGT 4 cut(s) 975, 984, 1281, 1419
TaqI TCGA 5 cut(s) 60, 748, 843, 1110, 1380
TasI AATT 3 cut(s) 195, 557, 1406
TatI WGTACW 1 cut(s) 168
TauI GCSGC 2 cut(s) 362, 1526
TfiI GAWTC 5 cut(s) 71, 263, 602, 990, 1031
Tru1I TTAA 3 cut(s) 354, 681, 1103
Tru9I TTAA 3 cut(s) 354, 681, 1103
TscAI CASTG 2 cut(s) 604, 1290
TseI GCWGC 3 cut(s) 80, 777, 1526
TspDTI ATGAA 5 cut(s) 296, 594, 1165, 1237, 1474
TspGWI ACGGA 1 cut(s) 704
TspRI CASTG 2 cut(s) 604, 1290
VpaK11BI GGWCC 5 cut(s) 236, 332, 520, 535, 1479
XbaI TCTAGA 1 cut(s) 305
XceI RCATGY 2 cut(s) 169, 834
XmiI GTMKAC 1 cut(s) 783
XmnI GAANNNNTTC 2 cut(s) 297, 644
XspI CTAG 5 cut(s) 306, 623, 731, 1088, 1272
ZraI GACGTC 1 cut(s) 1279
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.